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Pesquisa : G02.111.158 [Categoria DeCS]
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[PMID]:29431947
[Au] Autor:Lapko IV; Kiryakov VA; Pavlovskaya NA; Oshkoderov OA; Klimkina KV
[Ti] Título:[Choice of informative laboratory biomarkers for the early identification of changes in neurohumoral regulation and carbohydrate exchange in workers of the mining and mechanical engineering industry].
[So] Source:Gig Sanit;95(11):1061-5, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The diagnostic significance of hormones and integral indices of pituitary-adrenal, pituitary-thyroid and pituitary-gonadal system and carbohydrate metabolism (ACTH (corticotropin), aldosterone, cortisol, TSH (thyroid-stimulating hormone), free triiodothyronine (fT3), free thyroxine (fT4), luteinizing hormone (LH), follicle-stimulating hormone (FSH), total and free testosterone, insulin, integral pituitary-adrenal index (IPAI), the pituitary-thyroid index (PTI), indices of carbohydrate metabolism (Caro and HOMA-IR) was studied for the early diagnostics of disorders of neurohumoral regulation in workers of mining and mechanical engineering industries. The most informative indices, permitting to identify disorders of carbohydrate metabolism are established to be indices of insulin resistance (index Caro and index NOMA-IR) and the determination of insulin in serum. For the identification of changes in pituitary adrenal, pituitary-thyroid and pituitary-gonadal system in patients with vibration disease, sensory-neural hearing loss, comorbidity indexes IGNI, ITI, concentrations of LH and total testosterone are of the most diagnostically significance.
[Mh] Termos MeSH primário: Hormônio Adrenocorticotrópico/sangue
Hormônio Foliculoestimulante/sangue
Insulina/sangue
Doenças Profissionais
Tireotropina/sangue
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/sangue
Metabolismo dos Carboidratos/fisiologia
Indústrias Extrativas e de Processamento/métodos
Indústrias Extrativas e de Processamento/normas
Feminino
Seres Humanos
Sistema Hipotálamo-Hipofisário/metabolismo
Masculino
Meia-Idade
Doenças Profissionais/sangue
Doenças Profissionais/diagnóstico
Doenças Profissionais/etiologia
Doenças Profissionais/prevenção & controle
Saúde do Trabalhador
Sistema Hipófise-Suprarrenal/metabolismo
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Insulin); 9002-60-2 (Adrenocorticotropic Hormone); 9002-68-0 (Follicle Stimulating Hormone); 9002-71-5 (Thyrotropin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  2 / 20333 MEDLINE  
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[PMID]:29270996
[Au] Autor:Maldonado MR; Bracht L; de Sá-Nakanishi AB; Corrêa RCG; Comar JF; Peralta RM; Bracht A
[Ad] Endereço:Department of Biochemistry, University of Maringá, Maringá, Brazil.
[Ti] Título:Actions of p-synephrine on hepatic enzyme activities linked to carbohydrate metabolism and ATP levels in vivo and in the perfused rat liver.
[So] Source:Cell Biochem Funct;36(1):4-12, 2018 Jan.
[Is] ISSN:1099-0844
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:p-Synephrine is one of the main active components of the fruit of Citrus aurantium (bitter orange). Extracts of the bitter orange and other preparations containing p-synephrine have been used worldwide to promote weight loss and for sports performance. The purpose of the study was to measure the action of p-synephrine on hepatic enzyme activities linked to carbohydrate and energy metabolism and the levels of adenine mononucleotides. Enzymes and adenine mononucleotides were measured in the isolated perfused rat liver and in vivo after oral administration of the drug (50 and 300 mg/kg) by using standard techniques. p-Synephrine increased the activity of glycogen phosphorylase in vivo and in the perfused liver. It decreased, however, the activities of pyruvate kinase and pyruvate dehydrogenase also in vivo and in the perfused liver. p-Synephrine increased the hepatic pools of adenosine diphosphate and adenosine triphosphate. Stimulation of glycogen phosphorylase is consistent with the reported increased glycogenolysis in the perfused liver and increased glycemia in rats. The decrease in the pyruvate dehydrogenase activity indicates that p-synephrine is potentially capable of inhibiting the transformation of carbohydrates into lipids. The capability of increasing the adenosine triphosphate-adenosine diphosphate pool indicates a beneficial effect of p-synephrine on the cellular energetics.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Metabolismo dos Carboidratos/efeitos dos fármacos
Fígado/efeitos dos fármacos
Fígado/enzimologia
Sinefrina/farmacologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Citrus/química
Glicogênio Fosforilase/metabolismo
Fígado/irrigação sanguínea
Fígado/cirurgia
Masculino
Complexo Piruvato Desidrogenase/antagonistas & inibidores
Complexo Piruvato Desidrogenase/metabolismo
Piruvato Quinase/antagonistas & inibidores
Piruvato Quinase/metabolismo
Ratos
Ratos Wistar
Sinefrina/administração & dosagem
Sinefrina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pyruvate Dehydrogenase Complex); 8L70Q75FXE (Adenosine Triphosphate); EC 2.4.1.- (Glycogen Phosphorylase); EC 2.7.1.40 (Pyruvate Kinase); PEG5DP7434 (Synephrine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1002/cbf.3311


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[PMID]:28453664
[Au] Autor:Krag TO; Ruiz-Ruiz C; Vissing J
[Ad] Endereço:Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark.
[Ti] Título:Glycogen Synthesis in Glycogenin 1-Deficient Patients: A Role for Glycogenin 2 in Muscle.
[So] Source:J Clin Endocrinol Metab;102(8):2690-2700, 2017 Aug 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: Glycogen storage disease (GSD) type XV is a rare disease caused by mutations in the GYG1 gene that codes for the core molecule of muscle glycogen, glycogenin 1. Nonetheless, glycogen is present in muscles of glycogenin 1-deficient patients, suggesting an alternative for glycogen buildup. A likely candidate is glycogenin 2, an isoform expressed in the liver and heart but not in healthy skeletal muscle. Objective: We wanted to investigate the formation of glycogen and changes in glycogen metabolism in patients with GSD type XV. Design, Setting, and Patients: Two patients with mutations in the GYG1 gene were investigated for histopathology, ultrastructure, and expression of proteins involved in glycogen synthesis and metabolism. Results: Apart from occurrence of polyglucosan (PG) bodies in few fibers, glycogen appeared normal in most cells, and the concentration was normal in patients with GSD type XV. We found that glycogenin 1 was absent, but glycogenin 2 was present in the patients, whereas the opposite was the case in healthy controls. Electron microscopy revealed that glycogen was present between and not inside myofibrils in type II fibers, compromising the ultrastructure of these fibers, and only type I fibers contained PG bodies. We also found significant changes to the expression levels of several enzymes directly involved in glycogen and glucose metabolism. Conclusions: To our knowledge, this is the first report demonstrating expression of glycogenin 2 in glycogenin 1-deficient patients, suggesting that glycogenin 2 rescues the formation of glycogen in patients with glycogenin 1 deficiency.
[Mh] Termos MeSH primário: Glucosiltransferases/genética
Glucosiltransferases/metabolismo
Glicogênio/biossíntese
Glicoproteínas/genética
Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Idoso
Metabolismo dos Carboidratos
Estudos de Casos e Controles
Feminino
Glucanos/metabolismo
Glucose/metabolismo
Glicogênio/metabolismo
Glicogênio/ultraestrutura
Doença de Depósito de Glicogênio/genética
Glicoproteínas/metabolismo
Seres Humanos
Microscopia Eletrônica
Meia-Idade
Fibras Musculares de Contração Rápida/ultraestrutura
Músculo Esquelético/patologia
Músculo Esquelético/ultraestrutura
Miofibrilas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Glycoproteins); 0 (glycogenin); 9005-79-2 (Glycogen); 9012-72-0 (polyglucosan); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.186 (GYG2 protein, human); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00399


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[PMID]:29486760
[Au] Autor:Ji Y; Rong X; Lu Y
[Ad] Endereço:Department of Ophthalmology and Eye Institute, Eye and ENT Hospital of Fudan University, Key Laboratory of Myopia of State Health Ministry, and Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China.
[Ti] Título:Metabolic characterization of human aqueous humor in the cataract progression after pars plana vitrectomy.
[So] Source:BMC Ophthalmol;18(1):63, 2018 Feb 27.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: While pars plana vitrectomy (PPV) has become the third most commonly performed surgery in the world, it can also induce multiple post complications easily. Among them, cataract progression is the most frequent one that can lead to blindness eventually. METHODS: To understand the underlying mechanisms of post PPV cataract progression, we performed comprehensive metabolic characterization of aqueous humor (AH) samples from 20 cataract patients (10 post PPV complication and 10 none PPV cataract) by a non-targeted metabolomic analysis using gas chromatography combined with time-of-flight mass spectrometer (GC/TOF MS). RESULTS: A total of 263 metabolites were identified and eight of them are determined to be significantly different (VIP ≥ 1 and p ≤ 0.05) between post PPV group and none PPV control group. The significantly changed metabolites included glutaric acid and pelargonic acid that play key roles in the regulation of oxidative stress and inflammatory responses. Furthermore, we constructed a metabolic regulatory network in each group based on metabolite-metabolite correlations, which reveals key metabolic pathways and regulatory elements including amino acids and lipids metabolisms that are related to cataract progression. CONCLUSIONS: Altogether, this work discovered some potential metabolite biomarkers for post PPV cataract diagnostics, as well as casted some novel insights into the underlying mechanisms of cataract progression after PPV.
[Mh] Termos MeSH primário: Humor Aquoso/metabolismo
Catarata/metabolismo
Vitrectomia/efeitos adversos
[Mh] Termos MeSH secundário: Idoso
Aminoácidos/metabolismo
Metabolismo dos Carboidratos/fisiologia
Carboidratos
Estudos de Casos e Controles
Catarata/etiologia
Progressão da Doença
Feminino
Seres Humanos
Metabolismo dos Lipídeos/fisiologia
Masculino
Meia-Idade
Análise de Regressão
Acuidade Visual
Vitrectomia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Carbohydrates)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180301
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0729-y


  5 / 20333 MEDLINE  
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[PMID]:29350287
[Au] Autor:Luan W; Zhou Z; Ni X; Xia Y; Wang J; Yan Y; Xu B
[Ad] Endereço:Department of Plastic Surgery, Affiliated People's Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, Jiangsu, China.
[Ti] Título:Long non-coding RNA H19 promotes glucose metabolism and cell growth in malignant melanoma via miR-106a-5p/E2F3 axis.
[So] Source:J Cancer Res Clin Oncol;144(3):531-542, 2018 Mar.
[Is] ISSN:1432-1335
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: lncRNA H19 has been considered as an oncogenic lncRNA in many human tumours. In the present study, we identify the role and molecular mechanism of lncRNA H19 in melanoma. METHOD: QRT-PCR was used to detect the expression of lncRNA H19 and E2F3 was detected in melanoma tissues. Cell counting kit-8 (CCK8), representative metabolites analysis was used to explore the biological function of lncRNA H19, miR-106a-5p and E2F3 in melanoma cells. Bioinformatics, luciferase reporter assays, MS2-RIP and RNA pull-down assay was used to demonstrate the molecular mechanism of lncRNA H19 in melanoma. We further test the function of lncRNA H19 in vivo though Xenograft tumour assay. RESULTS: We found that lncRNA H19 was increased in melanoma tissue, and lncRNA H19 was correlated with poor prognosis of melanoma patients. miR-106a-5p acts as a tumour suppressor in melanoma by targeting E2F3. E2F3 affects the melanoma cell glucose metabolism and growth. We also demonstrated that lncRNA H19 may function as the sponge of miR-106a-5p to up-regulate E2F3 expression, and consequently promote the glucose metabolism and growth of melanoma. CONCLUSIONS: This result elucidates a new mechanism for lncRNA H19 in melanoma development and provides a survival indicator and potential therapeutic target for melanoma patients.
[Mh] Termos MeSH primário: Proliferação Celular/genética
Fator de Transcrição E2F3/fisiologia
Glucose/metabolismo
Melanoma
MicroRNAs/fisiologia
RNA Longo não Codificante/fisiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Metabolismo dos Carboidratos/genética
Linhagem Celular Tumoral
Fator de Transcrição E2F3/genética
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Masculino
Melanoma/genética
Melanoma/metabolismo
Melanoma/mortalidade
Melanoma/patologia
Camundongos
Camundongos Nus
MicroRNAs/genética
Meia-Idade
Transdução de Sinais/genética
Neoplasias Cutâneas/genética
Neoplasias Cutâneas/metabolismo
Neoplasias Cutâneas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (E2F3 Transcription Factor); 0 (E2F3 protein, human); 0 (H19 long non-coding RNA); 0 (MIRN106 microRNA, human); 0 (MicroRNAs); 0 (RNA, Long Noncoding); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1007/s00432-018-2582-z


  6 / 20333 MEDLINE  
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[PMID]:28449056
[Au] Autor:Aguilera-Alvarado GP; Sánchez-Nieto S
[Ad] Endereço:Departamento de Bioquímica, Facultad de Química, Conjunto E. Universidad Nacional Autónoma de México, Cd. Universitaria, Coyoacán, México 04510, DF, México.
[Ti] Título:Plant Hexokinases are Multifaceted Proteins.
[So] Source:Plant Cell Physiol;58(7):1151-1160, 2017 Jul 01.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Sugars are the main carbon and energy source in cells, but they can also act as signaling molecules that affect the whole plant life cycle. Certain tissues can produce sugars and supply them to others, and this plant tissue heterogeneity makes sugar signaling a highly complex process that requires elements capable of perceiving changes in sugar concentrations among different tissues, cell compartments and developmental stages. In plants, the regulatory effects of glucose (Glc) have been the most studied to date. The first Glc sensor identified in plants was hexokinase (HXK), which is currently recognized as a dual-function protein. In addition to its catalytic activity, this enzyme can also repress the expression of some photosynthetic genes in response to high internal Glc concentrations. Additionally, the catalytic activity of HXKs has a profound impact on cell metabolism and other sugar signaling pathways that depend on phosphorylated hexoses and intermediate glycolytic products. HXKs are the only proteins that are able to phosphorylate Glc in plants, since no evidence has been provided to date concerning the existence of a glucokinase. Moreover, the intracellular localization of HXKs seems to be crucial to their activity and sensor functions. Recently, two new and surprising functions have been described for HXKs. In this review, we discuss the versatility of HXKs in regard to their catalytic and glucose sensor activities, intracellular location, protein-protein and hormone interactions, as well as how these HXK characteristics influence plant growth and development, in an effort to understand this enzyme's role in improving plant productivity.
[Mh] Termos MeSH primário: Hexoquinase/metabolismo
Desenvolvimento Vegetal
Plantas/enzimologia
Transdução de Sinais
[Mh] Termos MeSH secundário: Metabolismo dos Carboidratos
Glucose/metabolismo
Hexoquinase/genética
Fosforilação
Fotossíntese
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Plant Proteins); EC 2.7.1.1 (Hexokinase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/pcp/pcx062


  7 / 20333 MEDLINE  
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[PMID]:29377659
[Au] Autor:Sharafetdinov KK; Plotnikova OA; Churicheva AM; Pilipenko VV; Alekseeva RI; Sentsova TB; Maltsev GY; Kochetkova AA; Vorobyova VM; Vorobyova IS
[Ti] Título:Assessment of efficacy of specialized food products with modified carbohydrate profile in patients with type 2 diabetes.
[So] Source:Vopr Pitan;85(6):103-9, 2016.
[Is] ISSN:0042-8833
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Objective - To evaluate the effect of diet therapy with the inclusion of specialized food product with a modified carbohydrate profile (fruit jelly) on glycemic and metabolic parameters in patients with type 2 diabetes mellitus. The study included 45 patients (aged 35 to 69 years) with type 2 diabetes with concomitant obesity I-III degree. The study was conducted in two stages. The first phase evaluated the effect of jelly intake on postprandial glycemia with determination of blood glucose levels on an empty stomach and 30, 60, 120 and 180 minutes after consumption of fruit jelly with maltitol, pectin and sublimated raspberry juice and traditional marmalade with fructose (control), containing 25 g of digestible carbohydrates. In the second phase the effectiveness of combination therapy during 2 weeks of the inclusion of fruit jelly with a modified carbohydrate profile in a standard hypocaloric diet (1550 kcal/day) has been assessed. It has been shown that the dynamics of postprandial glycemia in patients with type 2 diabetes after the consumption of fruit jelly with a modified carbohydrate profile was comparable to the dynamics of blood glucose after eating traditional marmalade with fructose. Inclusion of fruit jelly with a modified carbohydrate profile in the standard diet therapy of patients with type 2 diabetes during 2 weeks was accompanied by positive dynamics of carbohydrate and lipid metabolism, as well as a decrease in the content of lipid peroxidation products in blood plasma: reducing the level of conjugated dienes and malondialdehyde averaged 15.2 and 17.4% from baseline (p<0.05), respectively, while content of lipid peroxidation products in blood plasma did not changed in patients from the control group.
[Mh] Termos MeSH primário: Metabolismo dos Carboidratos/efeitos dos fármacos
Carboidratos da Dieta/administração & dosagem
Alimentos Especializados
Metabolismo dos Lipídeos/efeitos dos fármacos
Obesidade
[Mh] Termos MeSH secundário: Adulto
Idoso
Diabetes Mellitus Tipo 2/dietoterapia
Diabetes Mellitus Tipo 2/metabolismo
Diabetes Mellitus Tipo 2/patologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Obesidade/dietoterapia
Obesidade/metabolismo
Obesidade/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Carbohydrates)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE


  8 / 20333 MEDLINE  
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[PMID]:29351296
[Au] Autor:Passera A; Marcolungo L; Casati P; Brasca M; Quaglino F; Cantaloni C; Delledonne M
[Ad] Endereço:Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Milan, Italy.
[Ti] Título:Hybrid genome assembly and annotation of Paenibacillus pasadenensis strain R16 reveals insights on endophytic life style and antifungal activity.
[So] Source:PLoS One;13(1):e0189993, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria of the Paenibacillus genus are becoming important in many fields of science, including agriculture, for their positive effects on the health of plants. However, there are little information available on this genus compared to other bacteria (such as Bacillus or Pseudomonas), especially when considering genomic information. Sequencing the genomes of plant-beneficial bacteria is a crucial step to identify the genetic elements underlying the adaptation to life inside a plant host and, in particular, which of these features determine the differences between a helpful microorganism and a pathogenic one. In this study, we have characterized the genome of Paenibacillus pasadenensis, strain R16, recently investigated for its antifungal activities and plant-associated features. An hybrid assembly approach was used integrating the very precise reads obtained by Illumina technology and long fragments acquired with Oxford Nanopore Technology (ONT) sequencing. De novo genome assembly based solely on Illumina reads generated a relatively fragmented assembly of 5.72 Mbp in 99 ungapped sequences with an N50 length of 544 Kbp; hybrid assembly, integrating Illumina and ONT reads, improved the assembly quality, generating a genome of 5.75 Mbp, organized in 6 contigs with an N50 length of 3.4 Mbp. Annotation of the latter genome identified 4987 coding sequences, of which 1610 are hypothetical proteins. Enrichment analysis identified pathways of particular interest for the endophyte biology, including the chitin-utilization pathway and the incomplete siderophore pathway which hints at siderophore parasitism. In addition the analysis led to the identification of genes for the production of terpenes, as for example farnesol, that was hypothesized as the main antifungal molecule produced by the strain. The functional analysis on the genome confirmed several plant-associated, plant-growth promotion, and biocontrol traits of strain R16, thus adding insights in the genetic bases of these complex features, and of the Paenibacillus genus in general.
[Mh] Termos MeSH primário: Endófitos/genética
Fungos/patogenicidade
Genoma Bacteriano
Paenibacillus/genética
Plantas/microbiologia
[Mh] Termos MeSH secundário: Amino Açúcares/metabolismo
Metabolismo dos Carboidratos
DNA Bacteriano/genética
DNA Bacteriano/isolamento & purificação
Endófitos/metabolismo
Endófitos/fisiologia
Sequenciamento de Nucleotídeos em Larga Escala
Compostos Orgânicos/metabolismo
Paenibacillus/metabolismo
Paenibacillus/fisiologia
Desenvolvimento Vegetal
Sideróforos/biossíntese
Esporos Bacterianos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Sugars); 0 (DNA, Bacterial); 0 (Organic Chemicals); 0 (Siderophores)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189993


  9 / 20333 MEDLINE  
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[PMID]:29324867
[Au] Autor:Gao L; Zhao S; Lu X; He N; Zhu H; Dou J; Liu W
[Ad] Endereço:Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, China.
[Ti] Título:Comparative transcriptome analysis reveals key genes potentially related to soluble sugar and organic acid accumulation in watermelon.
[So] Source:PLoS One;13(1):e0190096, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Soluble sugars and organic acids are important components of fruit flavor and have a strong impact on the overall organoleptic quality of watermelon (Citrullus lanatus) fruit. Several studies have analyzed the expression levels of the genes related to soluble sugar accumulation and the dynamic changes in their content during watermelon fruit development and ripening. Nevertheless, to date, there have been no reports on the organic acid content in watermelon or the genes regulating their synthesis. In this study, the soluble sugars and organic acids in watermelon were measured and a comparative transcriptome analysis was performed to identify the key genes involved in the accumulation of these substances during fruit development and ripening. The watermelon cultivar '203Z' and its near-isogenic line (NIL) 'SW' (in the '203Z' background) were used as experimental materials. The results suggested that soluble sugar consist of fructose, glucose and sucrose while malic-, citric-, and oxalic acids are the primary organic acids in watermelon fruit. Several differentially expressed genes (DEGs) related to soluble sugar- and organic acid accumulation and metabolism were identified. These include the DEGs encoding raffinose synthase, sucrose synthase (SuSy), sucrose-phosphate synthase (SPSs), insoluble acid invertases (IAI), NAD-dependent malate dehydrogenase (NAD-cyt MDH), aluminum-activated malate transporter (ALMT), and citrate synthase (CS). This is the first report addressing comparative transcriptome analysis via NILs materials in watermelon fruit. These findings provide an important basis for understanding the molecular mechanism that leads to soluble sugar and organic acid accumulation and metabolism during watermelon fruit development and ripening.
[Mh] Termos MeSH primário: Ácidos/metabolismo
Metabolismo dos Carboidratos
Citrullus/genética
Compostos Orgânicos/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Citrullus/metabolismo
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de RNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acids); 0 (Organic Chemicals)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190096


  10 / 20333 MEDLINE  
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[PMID]:29232716
[Au] Autor:Hu Y; Zhong X; Liu X; Lou B; Zhou C; Wang X
[Ad] Endereço:National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, P. R. China.
[Ti] Título:Comparative transcriptome analysis unveils the tolerance mechanisms of Citrus hystrix in response to 'Candidatus Liberibacter asiaticus' infection.
[So] Source:PLoS One;12(12):e0189229, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Citrus Huanglongbing (HLB), a highly devastating citrus disease, is associated with 'Candidatus Liberibacter asiacitus' (CLas), a member of phloem-inhabiting α-proteobacteria. HLB can affect all cultivated citrus and no cure is currently available. Previous studies showed that Kaffir lime (Citrus hystrix), primarily grown in South Asia and Southeast Asia, was tolerant to HLB but the molecular mechanism remains unknown. In this study, gene expression profiling experiments were performed on HLB-tolerant C. hystrix and HLB-susceptible C. sinensis three months after inoculation with CLas using RNA-seq data. Differentially expressed genes (DEGs) in the two citrus cultivars were mainly involved in diverse cellular functions including carbohydrate metabolism, photosynthesis, cell wall metabolism, secondary metabolism, hormone metabolism and oxidation/reduction processes. Notably, starch synthesis and photosynthesis process were not disturbed in CLas-infected C. hystrix. Most of the DEGs involved in cell wall metabolism and secondary metabolism were up-regulated in C. hystrix. In addition, the activation of peroxidases, Cu/Zn-SOD and POD4, may also enhance the tolerance of C. hystrix to CLas. This study provides an insight into the host response of HLB-tolerant citrus cultivar to CLas. C. hystrix is potentially useful for HLB-tolerant/resistant citrus breeding in the future.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Citrus/fisiologia
Doenças das Plantas/microbiologia
Rhizobiaceae/patogenicidade
Transcriptoma
[Mh] Termos MeSH secundário: Metabolismo dos Carboidratos
Citrus/metabolismo
Citrus/microbiologia
Fotossíntese
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189229



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