Base de dados : MEDLINE
Pesquisa : G02.111.375 [Categoria DeCS]
Referências encontradas : 890 [refinar]
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[PMID]:29458459
[Au] Autor:Klotz F; Brinkhoff T; Freese HM; Wietz M; Teske A; Simon M; Giebel HA
[Ad] Endereço:1​Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, D-26111 Oldenburg, Germany.
[Ti] Título:Tritonibacter horizontis gen. nov., sp. nov., a member of the Rhodobacteraceae, isolated from the Deepwater Horizon oil spill.
[So] Source:Int J Syst Evol Microbiol;68(3):736-744, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A heterotrophic, Gram-stain-negative, aerobic, sodium-requiring and motile bacterium was isolated from oil-contaminated surface water of the Gulf of Mexico during the Deepwater Horizon oil spill. Strain O3.65 showed highest 16S rRNA gene sequence similarity to Phaeobacter gallaeciensis BS107 and Phaeobacter inhibens T5 , both with 98.3 %, respectively. Based on complete genome analysis, highest similarity was observed to species of the genus Ruegeria. Strain O3.65 exhibited a broad salinity, temperature and pH range of 0.5-10 % NaCl, 4-45 °C and 5.5-9.0, respectively. The DNA G+C content of strain O3.65 was 61.5 mol%. The major respiratory lipoquinone was ubiquinone-10 (Q-10), the most dominant fatty acids (>1 %) comprised 18 : 1ω7c and 18 : 1ω7c 11-methyl, 10 : 0 3OH, 12 : 1 3OH, 14 : 1 3OH/3-oxo-14 : 0, 16 : 0, 16 : 0 2OH, 18 : 1 2OH and 12 : 1. The polar lipid pattern indicated presence of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids and seven unidentified lipids. On Difco marine broth agar, strain O3.65 formed smooth, shiny white to beige and convex colonies with regular edges. Phylogenetic, phylogenomic and phenotypic differences revealed that strain O3.65 represents a new species of a novel genus within the family Rhodobacteraceae, for which we propose the name Tritonibacter horizontis gen. nov., sp. nov. The type strain of the type species is O3.65 (=DSM 101689 =LMG 29740 ).
[Mh] Termos MeSH primário: Poluição por Petróleo
Filogenia
Rhodobacteraceae/classificação
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Golfo do México
Processos Heterotróficos
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Rhodobacteraceae/genética
Rhodobacteraceae/isolamento & purificação
Análise de Sequência de DNA
Ubiquinona/química
Poluentes Químicos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Water Pollutants, Chemical); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002573


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[PMID]:29117591
[Au] Autor:Zhai S; Ji M; Zhao Y; Pavlostathis SG; Zhao Q
[Ad] Endereço:School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China; School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0512, United States.
[Ti] Título:Effects of salinity and COD/N on denitrification and bacterial community in dicyclic-type electrode based biofilm reactor.
[So] Source:Chemosphere;192:328-336, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A dicyclic-type electrode based biofilm electrode reactor (BER) was developed for advanced nitrate removal from saline municipal wastewater. The denitrification efficiency was evaluated with a synthetic feed (NO -N, 20 mg L ) under different salinity and COD to nitrogen ratios (COD/N). As the salinity increased from 0% to 1.0%, the denitrification performance of both the traditional biofilm reactor (BR) and BER was inhibited; however, the BER showed better adaptation and ability to recover. The BER achieved a high nitrate removal efficiency (≥90%) at a salinity of 1.0% and a low COD/N of 2.5 (theoretical stoichiometric 2.86 ignoring microbial growth). The abundance of Methylotenera mobilis in BR and Clostridium sticklandii in BER was higher than in the initial sludge sample used as inoculum. Likewise, the abundance of napA, nirS and nosZ genes increased as the COD/N further decreased. Under high salinity stress, the BER had a higher denitrification efficiency and the consumption of the organic carbon source (i.e., methanol) was reduced compared to BR. The cooperation between heterotrophic and autotrophic denitrifiers in the BER system provides a more efficient and feasible solution for nitrate removal from saline municipal wastewater.
[Mh] Termos MeSH primário: Biofilmes
Reatores Biológicos/microbiologia
Desnitrificação
Salinidade
[Mh] Termos MeSH secundário: Processos Autotróficos
Reatores Biológicos/normas
Processos Heterotróficos
Nitratos/isolamento & purificação
Nitrogênio/análise
Esgotos/microbiologia
Águas Residuais/química
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrates); 0 (Sewage); 0 (Waste Water); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171109
[St] Status:MEDLINE


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[PMID]:29364604
[Au] Autor:Galach'yants AD; Bel'kova NL; Sukhanova EV; Romanovskaya VA; Gladka GV; Bedoshvili ED; Parfenova VV
[Ti] Título:[Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria. Isolated from Lake Baikal Neuston.]
[So] Source:Mikrobiologiia;85(5):568-579, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For heterotrophic microorganisms (44 strains) isolated-from the surface film of Lake Baikal, iden- tification was carried out and their. physiological and biochemical characteristics were determined. Com- pared to the water column, diversity of cultured heterotrophs was low, indicating formation of stable micro- bial communities at the air-water interphase interface. Heterotrophic bacteria isolated from the surface mi- crolayer exhibited the enzymatic activity comparable to that for strains form other biofilm associations. Deinococcusfi6us strain NA202 'vas the most active component of the community, capable of utilization of the broadest spectrum of mono- and disaccharides,'sugars, and amino acids. This strain possessed the highest diversity of extracellular enzymes and was the most resistant to UV radiation. The physiological and bio- chemical properties of this strain may-be responsible for its adaptation to survival in extreme conditions of the surface microlayer. Our results improve our understanding of occurrence of UV-resistant strains in freshwater ecosystems.
[Mh] Termos MeSH primário: Deinococcus/metabolismo
Processos Heterotróficos/fisiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/efeitos da radiação
Aminoácidos/metabolismo
Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Bacteroidetes/efeitos da radiação
Betaproteobacteria/classificação
Betaproteobacteria/genética
Betaproteobacteria/isolamento & purificação
Betaproteobacteria/efeitos da radiação
Biodiversidade
Deinococcus/classificação
Deinococcus/isolamento & purificação
Deinococcus/efeitos da radiação
Deltaproteobacteria/classificação
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Deltaproteobacteria/efeitos da radiação
Dissacarídeos/metabolismo
Ecossistema
Firmicutes/classificação
Firmicutes/genética
Firmicutes/isolamento & purificação
Firmicutes/efeitos da radiação
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/efeitos da radiação
Processos Heterotróficos/efeitos da radiação
Consórcios Microbianos/efeitos da radiação
Monossacarídeos/metabolismo
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Proteobactérias/efeitos da radiação
Sibéria
Propriedades de Superfície
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Disaccharides); 0 (Monosaccharides); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29364606
[Au] Autor:Rumyantseva KV; Kosolapova NG; Kosolapov DB
[Ti] Título:[Relations between Bacterioplankton, Heterotrophic Nanoflagellates, and Virioplankton in the Littoral Zone of a LarRe Plain Reservoir:. ImDact of Bird Colonies.]
[So] Source:Mikrobiologiia;85(5):588-597, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Interactions of the main components of microbial planktonic food web (bacteria, heterotrophic nanoflagellates, and viruses) were studied in a protected overgrown littoral zone of the Rybinsk Reservoir (Upper Volga).. The effect of bird colonial, settlements (the Laridae family) on these processes was deter- mined. The following systems exhibited significant negative correlations: "heterotrophic nanoflagellates- large rod-shaped bacteria" ("predator-prey"), "viruses-bacteriophages-bacterial products" ("parasite-. host") and "heterotrophic nanoflagellates-viruses-bacteriophages." Relations between biotic factors con- trolling bacterial development were more pronounced outside the zone affected by colonial bird settlements. Near the bird colony the role of viruses in mortality of planktonic bacteria increased. Reproduction of bacte- rial cells accelerated in response to the increase in feeding activity of heterotrophic nanoflagellates. Viruses- bacteriophages and heterotrophic nanoflagellates probably eliminate different targets until medium-sized cells become predominant in the bacterial community. Then heterotrophic nanoflagellates consume bacterial cells infected with viruses.
[Mh] Termos MeSH primário: Bactérias/crescimento & desenvolvimento
Charadriiformes/fisiologia
Dinoflagelados/crescimento & desenvolvimento
Processos Heterotróficos/fisiologia
Plâncton/crescimento & desenvolvimento
Vírus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Bactérias/virologia
Carga Bacteriana
Contagem de Células
Dinoflagelados/microbiologia
Dinoflagelados/virologia
Ecossistema
Cadeia Alimentar
Plâncton/microbiologia
Plâncton/virologia
Tanques/microbiologia
Tanques/virologia
Federação Russa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29324779
[Au] Autor:Price MN; Zane GM; Kuehl JV; Melnyk RA; Wall JD; Deutschbauer AM; Arkin AP
[Ad] Endereço:Environmental Genomics & Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America.
[Ti] Título:Filling gaps in bacterial amino acid biosynthesis pathways with high-throughput genetics.
[So] Source:PLoS Genet;14(1):e1007147, 2018 01.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For many bacteria with sequenced genomes, we do not understand how they synthesize some amino acids. This makes it challenging to reconstruct their metabolism, and has led to speculation that bacteria might be cross-feeding amino acids. We studied heterotrophic bacteria from 10 different genera that grow without added amino acids even though an automated tool predicts that the bacteria have gaps in their amino acid synthesis pathways. Across these bacteria, there were 11 gaps in their amino acid biosynthesis pathways that we could not fill using current knowledge. Using genome-wide mutant fitness data, we identified novel enzymes that fill 9 of the 11 gaps and hence explain the biosynthesis of methionine, threonine, serine, or histidine by bacteria from six genera. We also found that the sulfate-reducing bacterium Desulfovibrio vulgaris synthesizes homocysteine (which is a precursor to methionine) by using DUF39, NIL/ferredoxin, and COG2122 proteins, and that homoserine is not an intermediate in this pathway. Our results suggest that most free-living bacteria can likely make all 20 amino acids and illustrate how high-throughput genetics can uncover previously-unknown amino acid biosynthesis genes.
[Mh] Termos MeSH primário: Aminoácidos/biossíntese
Aminoácidos/genética
Bactérias/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Processos Heterotróficos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Histidina/biossíntese
Metionina/biossíntese
Análise de Sequência de DNA/métodos
Serina/biossíntese
Treonina/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 0 (Bacterial Proteins); 2ZD004190S (Threonine); 452VLY9402 (Serine); 4QD397987E (Histidine); AE28F7PNPL (Methionine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007147


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[PMID]:28470424
[Au] Autor:Hong X; Chen Z; Zhao C; Yang S
[Ad] Endereço:Department of Bioengineering and Biotechnology, Huaqiao University, No. 668 Jimei Ave, Xiamen, 361021, People's Republic of China.
[Ti] Título:Nitrogen transformation under different dissolved oxygen levels by the anoxygenic phototrophic bacterium Marichromatium gracile.
[So] Source:World J Microbiol Biotechnol;33(6):113, 2017 Jun.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Marichromatium gracile: YL28 (M. gracile YL28) is an anoxygenic phototrophic bacterial strain that utilizes ammonia, nitrate, or nitrite as its sole nitrogen source during growth. In this study, we investigated the removal and transformation of ammonium, nitrate, and nitrite by M. gracile YL28 grown in a combinatorial culture system of sodium acetate-ammonium, sodium acetate-nitrate and sodium acetate-nitrite in response to different initial dissolved oxygen (DO) levels. In the sodium acetate-ammonium system under aerobic conditions (initial DO = 7.20-7.25 mg/L), we detected a continuous accumulation of nitrate and nitrite. However, under semi-anaerobic conditions (initial DO = 4.08-4.26 mg/L), we observed a temporary accumulation of nitrate and nitrite. Interestingly, under anaerobic conditions (initial DO = 0.36-0.67 mg/L), there was little accumulation of nitrate and nitrite, but an increase in nitrous oxide production. In the sodium acetate-nitrite system, nitrite levels declined slightly under aerobic conditions, and nitrite was completely removed under semi-anaerobic and anaerobic conditions. In addition, M. gracile YL28 was able to grow using nitrite as the sole nitrogen source in situations when nitrogen gas produced by denitrification was eliminated. Taken together, the data indicate that M. gracile YL28 performs simultaneous heterotrophic nitrification and denitrification at low-DO levels and uses nitrite as the sole nitrogen source for growth. Our study is the first to demonstrate that anoxygenic phototrophic bacteria perform heterotrophic ammonia-oxidization and denitrification under anaerobic conditions.
[Mh] Termos MeSH primário: Anaerobiose/fisiologia
Chromatiaceae/metabolismo
Nitrogênio/metabolismo
Oxigênio/metabolismo
Processos Fototróficos/fisiologia
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Aerobiose/fisiologia
Amônia/metabolismo
Compostos de Amônio/metabolismo
Bactérias
Chromatiaceae/crescimento & desenvolvimento
Desnitrificação
Processos Heterotróficos/fisiologia
Cinética
Nitratos/metabolismo
Nitrificação
Nitritos/metabolismo
Óxido Nitroso/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Ammonium Compounds); 0 (Nitrates); 0 (Nitrites); 7664-41-7 (Ammonia); K50XQU1029 (Nitrous Oxide); N762921K75 (Nitrogen); RRE756S6Q2 (ammonium acetate); S88TT14065 (Oxygen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2280-z


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[PMID]:28838232
[Au] Autor:Chen HH; Jiang JG
[Ad] Endereço:College of Food Science and Engineering, South China University of Technology , Guangzhou, Guangdong 510640, People's Republic of China.
[Ti] Título:Lipid Accumulation Mechanisms in Auto- and Heterotrophic Microalgae.
[So] Source:J Agric Food Chem;65(37):8099-8110, 2017 Sep 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microalgae lipids have attracted great attention in the world as a result of their potential use for biodiesel productions. Microalgae are cultivated in photoautotrophic conditions in most cases, but several species are able to grow under heterotrophic conditions, in which microalgae are cultivated in the dark where the cell growth and reproduction are supported by organic carbons. This perspective is covering the related studies concerning the difference between hetero- and autotrophic cultivation of microalgae. The auto- and heterotrophic central carbon metabolic pathways in microalgae are described, and the catalyzing reactions of several key metabolic enzymes and their corresponding changes in the protein level are summarized. Under adverse environmental conditions, such as nutrient deprivation, microalgae have the ability to highly store energy by forming triacylglycerol (TAG), the reason for which is analyzed. In addition, the biosynthesis of fatty acids and TAGs and their difference between auto- and heterotrophic conditions are compared at the molecular level. The positive regulatory enzymes, such as glucose transporter protein, fructose-1,6-bisphosphate aldolase, and glycerol-3-phosphate dehydrogenase, and the negative regulation enzymes, such as triose phosphate isomerase, played a crucial role in the lipid accumulation auto- and heterotrophic conditions.
[Mh] Termos MeSH primário: Lipídeos/biossíntese
Microalgas/metabolismo
[Mh] Termos MeSH secundário: Processos Autotróficos
Ácidos Graxos/biossíntese
Processos Heterotróficos
Microalgas/crescimento & desenvolvimento
Triglicerídeos/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Lipids); 0 (Triglycerides)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03495


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[PMID]:28803098
[Au] Autor:Rout PR; Bhunia P; Dash RR
[Ad] Endereço:School of Infrastructure, Indian Institute of Technology Bhubaneswar, Odisha 751 013, India.
[Ti] Título:Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal.
[So] Source:Bioresour Technol;244(Pt 1):484-495, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A newly isolated GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal was identified as Bacillus cereus GS-5 based on its phenotypic and phylogenetic characteristics. The isolate had exhibited efficient NH -N, NO -N, NO -N and PO -P removal from nutrient spiked real domestic wastewater with average rates of 2.62, 2.69, 1.16 and 0.42mgL h , respectively under aerobic condition. Metabolic inhibitor based mass balance analysis indicated that dinitrogen gas (41%), intracellular nitrogen (29%) and intracellular phosphorous (60%) were the major fates of the initial NH -N and PO -P. The successfully expression of hydroxylamine oxidase (hao), nitrate reductase (nar), nitrite reductase (nir) and poly phosphate kinase (ppk) enzyme in the cell free extracts and PCR amplification of nar, nir and ppk genes in the isolated strain provided further evidences for the nutrient removal possibility. A possible pathway of for nitrogen removal by GS-5 is suggested.
[Mh] Termos MeSH primário: Desnitrificação
Águas Residuais
[Mh] Termos MeSH secundário: Aerobiose
Bacillus cereus
Processos Heterotróficos
Nitrificação
Nitrogênio
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); N762921K75 (Nitrogen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170814
[St] Status:MEDLINE


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[PMID]:28792373
[Au] Autor:Beurmann S; Ushijima B; Svoboda CM; Videau P; Smith AM; Donachie SP; Aeby GS; Callahan SM
[Ad] Endereço:†â€‹Present address: Institute for GenomeSciences, School of Medicine, University of Maryland, Baltimore, MD 21201, USA. 2​Hawai'i Institute of Marine Biology, 46-007 Lilipuna Road, Kane'ohe, HI 96744, USA 1​Department of Microbiology, University of Hawai'i at Manoa, 2538 McCarthy Mall, Snyder H
[Ti] Título:Pseudoalteromonas piratica sp. nov., a budding, prosthecate bacterium from diseased Montipora capitata, and emended description of the genus Pseudoalteromonas.
[So] Source:Int J Syst Evol Microbiol;67(8):2683-2688, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, motile, rod-shaped bacterium designated OCN003T was cultivated from mucus taken from a diseased colony of the coral Montipora capitata in Kane'ohe Bay, O'ahu, Hawai'i. Colonies of OCN003T were pale yellow, 1-3 mm in diameter, convex, smooth and entire. The strain was heterotrophic, strictly aerobic and strictly halophilic. Cells of OCN003T produced buds on peritrichous prosthecae. Growth occurred within the pH range of 5.5 to 10, and the temperature range of 14 to 39 °C. Major fatty acids were 16 : 1ω7c, 16 : 0, 18 : 1ω7c, 17 : 1ω8c, 12 : 0 3-OH and 17 : 0. Phylogenetic analysis of 1399 nucleotides of the 16S rRNA gene nucleotide sequence and a multi-locus sequence analysis of three genes placed OCN003T in the genus Pseudoalteromonas and indicated that the nearest relatives described are Pseudoalteromonas spongiae, P. luteoviolacea, P. ruthenica and P. phenolica(97-99 % sequence identity). The DNA G+C content of the strain's genome was 40.0 mol%. Based on in silico DNA-DNA hybridization and phenotypic differences from related type strains, we propose that OCN003T represents the type strain of a novel species in the genus Pseudoalteromonas, proposed as Pseudoalteromonas piratica sp. nov. OCN003T (=CCOS1042T=CIP 111189T). An emended description of the genus Pseudoalteromonas is presented.
[Mh] Termos MeSH primário: Antozoários/microbiologia
Filogenia
Pseudoalteromonas/classificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hawaii
Processos Heterotróficos
Hibridização de Ácido Nucleico
Pigmentação
Pseudoalteromonas/genética
Pseudoalteromonas/isolamento & purificação
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001995


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[PMID]:28704687
[Au] Autor:Chakraborty K; Thilakan B; Raola VK
[Ad] Endereço:Marine Bioprospecting Section of Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, 682018, Kerala, India. Electronic address: kajal_cmfri@yahoo.com.
[Ti] Título:Antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium Bacillus subtilis MTCC 10403.
[So] Source:Phytochemistry;142:112-125, 2017 Oct.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Brown seaweed Anthophycus longifolius (Turner) Kützing (family Sargassaceae) associated heterotrophic bacterium Bacillus subtilis MTCC 10403 was found to be a potent isolate with broad range of antibacterial activity against important perceptive food pathogens Vibrio parahaemolyticus, V. vulnificus, and Aeromonas hydrophila. This bacterium was positive for polyketide synthetase gene (KC589397), and therefore, was selected to bioprospect specialized metabolites bearing polyketide backbone. Bioactivity-guided chromatographic fractionation of the ethyl acetate extract of the seaweed-associated bacterium segregated four homologous polyketide furanoterpenoids with potential antibacterial activities against clinically important pathogens. The minimum inhibitory concentration (MIC) assay showed that the referral antibiotics tetracycline and ampicillin were active at 25 µg/mL against the test pathogens, whereas the previously undescribed (4E)-methyl 13-((16-(furan-2-yl) ethyl)-octahydro-7-hydroxy-4-((E)-23-methylbut-21-enyl)-2H-chromen-6-yl)-4-methylpent-4-enoate (compound 1) and methyl 3-(hexahydro-9-((E)-3-methylpent-1-enyl)-4H-furo[3,2-g]isochromen-6-yl) propanoate (compound 3) displayed antibacterial activities against the test pathogens at a lesser concentration (MIC < 7 µg/mL). The title compounds were characterized by comprehensive nuclear magnetic resonance and mass spectroscopic experiments. Polyketide synthase catalyzed putative biosynthetic mechanism additionally corroborated the structural ascriptions of the hitherto undescribed furanoterpenoids from seaweed-associated bacterial symbiont. The electronic and hydrophobic parameters appeared to hold a conspicuous part in directing the antibacterial properties of the compounds. Seaweed-associated B. subtilis MTCC 10403 demonstrated to represent a potential source of antimicrobial polyketides for pharmaceutical applications.
[Mh] Termos MeSH primário: Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Policetídeos/farmacologia
Terpenos/isolamento & purificação
Terpenos/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Anti-Infecciosos/química
Bacillus subtilis/metabolismo
Processos Heterotróficos
Testes de Sensibilidade Microbiana
Feófitas/química
Policetídeo Sintases/metabolismo
Policetídeos/química
Policetídeos/isolamento & purificação
Alga Marinha/metabolismo
Terpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Polyketides); 0 (Terpenes); 79956-01-7 (Polyketide Synthases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE



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