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Pesquisa : G02.111.570.080.689.675.850 [Categoria DeCS]
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[PMID]:29176831
[Au] Autor:Watanabe K; Kokubo T
[Ad] Endereço:Molecular and Cellular Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, Japan.
[Ti] Título:SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae.
[So] Source:PLoS One;12(11):e0188435, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Saccharomyces cerevisiae, core promoters of class II genes contain a TATA element, either a TATA box (TATA[A/T]A[A/T][A/G]) or TATA-like element (1 or 2 bp mismatched version of the TATA box). The TATA element directs the assembly of the preinitiation complex (PIC) to ensure accurate transcriptional initiation. It has been proposed the PIC is assembled by two distinct pathways in which TBP is delivered by TFIID or SAGA, leading to the widely accepted model that these complexes mediate transcription mainly from TATA-like element- or TATA box-containing promoters, respectively. Although both complexes are involved in transcription of nearly all class II genes, it remains unclear how efficiently SAGA mediates transcription from TATA-like element-containing promoters independently of TFIID. We found that transcription from the TATA box-containing AGP1 promoter was greatly stimulated in a Spt3p-dependent manner after inactivation of Taf1p/TFIID. Thus, this promoter provides a novel experimental system in which to evaluate SAGA-mediated transcription from TATA-like element(s). We quantitatively measured transcription from various TATA-like elements in the Taf1p-dependent CYC1 promoter and Taf1p-independent AGP1 promoter. The results revealed that SAGA could mediate transcription from at least some TATA-like elements independently of Taf1p/TFIID, and that Taf1p-dependence or -independence is highly robust with respect to variation of the TATA sequence. Furthermore, chimeric promoter mapping revealed that Taf1p-dependence or independence was conferred by the upstream activating sequence (UAS), whereas Spt3p-dependent transcriptional stimulation after inactivation of Taf1p/TFIID was specific to the AGP1 promoter and dependent on core promoter regions other than the TATA box. These results suggest that TFIID and/or SAGA are regulated in two steps: the UAS first specifies TFIID or SAGA as the predominant factor on a given promoter, and then the core promoter structure guides the pertinent factor to conduct transcription in an appropriate manner.
[Mh] Termos MeSH primário: Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/genética
TATA Box/genética
Fatores Associados à Proteína de Ligação a TATA/metabolismo
Transativadores/metabolismo
Fator de Transcrição TFIID/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Sequência de Bases
Regulação Fúngica da Expressão Gênica
Genes Reporter
Cinética
Mutação/genética
Proteínas de Saccharomyces cerevisiae/genética
Fatores Associados à Proteína de Ligação a TATA/genética
Temperatura Ambiente
Fator de Transcrição TFIID/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (SAGA complex, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 0 (TAF1 protein, S cerevisiae); 0 (TATA-Binding Protein Associated Factors); 0 (Trans-Activators); 0 (Transcription Factor TFIID)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188435


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[PMID]:28735898
[Au] Autor:Krebs AR; Imanci D; Hoerner L; Gaidatzis D; Burger L; Schübeler D
[Ad] Endereço:Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland. Electronic address: arnaud.krebs@fmi.ch.
[Ti] Título:Genome-wide Single-Molecule Footprinting Reveals High RNA Polymerase II Turnover at Paused Promoters.
[So] Source:Mol Cell;67(3):411-422.e4, 2017 Aug 03.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transcription initiation entails chromatin opening followed by pre-initiation complex formation and RNA polymerase II recruitment. Subsequent polymerase elongation requires additional signals, resulting in increased residence time downstream of the start site, a phenomenon referred to as pausing. Here, we harnessed single-molecule footprinting to quantify distinct steps of initiation in vivo throughout the Drosophila genome. This identifies the impact of promoter structure on initiation dynamics in relation to nucleosomal occupancy. Additionally, perturbation of transcriptional initiation reveals an unexpectedly high turnover of polymerases at paused promoters-an observation confirmed at the level of nascent RNAs. These observations argue that absence of elongation is largely caused by premature termination rather than by stable polymerase stalling. In support of this non-processive model, we observe that induction of the paused heat shock promoter depends on continuous initiation. Our study provides a framework to quantify protein binding at single-molecule resolution and refines concepts of transcriptional pausing.
[Mh] Termos MeSH primário: DNA/metabolismo
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/enzimologia
Regiões Promotoras Genéticas
RNA Polimerase II/metabolismo
RNA/biossíntese
Imagem Individual de Molécula
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
DNA/genética
Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Estudo de Associação Genômica Ampla
Proteínas de Choque Térmico HSP70/genética
Proteínas de Choque Térmico HSP70/metabolismo
Meia-Vida
Cinética
Ligação Proteica
Estabilidade Proteica
Proteólise
RNA/genética
RNA Polimerase II/genética
TATA Box
Sítio de Iniciação de Transcrição
Iniciação da Transcrição Genética
Terminação da Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (HSP70 Heat-Shock Proteins); 63231-63-0 (RNA); 9007-49-2 (DNA); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE


  3 / 3147 MEDLINE  
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[PMID]:28539359
[Au] Autor:Wang J; Zhao S; He W; Wei Y; Zhang Y; Pegg H; Shore P; Roberts SGE; Deng W
[Ad] Endereço:From the Institute of Biology and Medicine, Wuhan University of Science and Technology, Wuhan City, Hubei Province 430065, China.
[Ti] Título:A transcription factor IIA-binding site differentially regulates RNA polymerase II-mediated transcription in a promoter context-dependent manner.
[So] Source:J Biol Chem;292(28):11873-11885, 2017 Jul 14.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA polymerase II (pol II) is required for the transcription of all protein-coding genes and as such represents a major enzyme whose activity is tightly regulated. Transcriptional initiation therefore requires numerous general transcriptional factors and cofactors that associate with pol II at the core promoter to form a pre-initiation complex. Transcription factor IIA (TFIIA) is a general cofactor that binds TFIID and stabilizes the TFIID-DNA complex during transcription initiation. Previous studies showed that TFIIA can make contact with the DNA sequence upstream or downstream of the TATA box, and that the region bound by TFIIA could overlap with the elements recognized by another factor, TFIIB, at adenovirus major late core promoter. Whether core promoters contain a DNA motif recognized by TFIIA remains unknown. Here we have identified a core promoter element upstream of the TATA box that is recognized by TFIIA. A search of the human promoter database revealed that many natural promoters contain a TFIIA recognition element (IIARE). We show that the IIARE enhances TFIIA-promoter binding and enhances the activity of TATA-containing promoters, but represses or activates promoters that lack a TATA box. Chromatin immunoprecipitation assays revealed that the IIARE activates transcription by increasing the recruitment of pol II, TFIIA, TAF4, and P300 at TATA-dependent promoters. These findings extend our understanding of the role of TFIIA in transcription, and provide new insights into the regulatory mechanism of core promoter elements in gene transcription by pol II.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica
Regiões Promotoras Genéticas
RNA Polimerase II/metabolismo
Elementos de Resposta
TATA Box
Fatores Associados à Proteína de Ligação a TATA/metabolismo
Fator de Transcrição TFIIA/metabolismo
Fator de Transcrição TFIID/metabolismo
[Mh] Termos MeSH secundário: Sítios de Ligação
Imunoprecipitação da Cromatina
DNA Recombinante
Proteína p300 Associada a E1A/química
Proteína p300 Associada a E1A/metabolismo
Genes Reporter
Células HEK293
Seres Humanos
Mutagênese Sítio-Dirigida
Mutação
Motivos de Nucleotídeos
Subunidades Proteicas/química
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
RNA Polimerase II/química
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Fatores Associados à Proteína de Ligação a TATA/química
Proteína de Ligação a TATA-Box/química
Proteína de Ligação a TATA-Box/genética
Proteína de Ligação a TATA-Box/metabolismo
Fator de Transcrição TFIIA/química
Fator de Transcrição TFIIA/genética
Fator de Transcrição TFIID/química
Fatores Estimuladores Upstream/química
Fatores Estimuladores Upstream/genética
Fatores Estimuladores Upstream/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Recombinant); 0 (Protein Subunits); 0 (Recombinant Proteins); 0 (TAF4 protein, human); 0 (TATA-Binding Protein Associated Factors); 0 (TATA-Box Binding Protein); 0 (TBP protein, human); 0 (Transcription Factor TFIIA); 0 (Transcription Factor TFIID); 0 (USF1 protein, human); 0 (Upstream Stimulatory Factors); EC 2.3.1.48 (E1A-Associated p300 Protein); EC 2.3.1.48 (EP300 protein, human); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.770412


  4 / 3147 MEDLINE  
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[PMID]:28411686
[Au] Autor:Jiang JF; Lei F; Yuan ZY; Wang YG; Wang XP; Yan XJ; Yu X; Xing DM; DU LJ
[Ad] Endereço:MOE (Ministry of Education) Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
[Ti] Título:Mechanism underlying berberine's effects on HSP70/TNFα under heat stress: Correlation with the TATA boxes.
[So] Source:Chin J Nat Med;15(3):178-191, 2017 Mar.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Heat stress can stimulate an increase in body temperature, which is correlated with increased expression of heat shock protein 70 (HSP70) and tumor necrosis factor α (TNFα). The exact mechanism underlying the HSP70 and TNFα induction is unclear. Berberine (BBR) can significantly inhibit the temperature rise caused by heat stress, but the mechanism responsible for the BBR effect on HSP70 and TNFα signaling has not been investigated. The aim of the present study was to explore the relationship between the expression of HSP70 and TNFα and the effects of BBR under heat conditions, using in vivo and in vitro models. The expression levels of HSP70 and TNFα were determined using RT-PCR and Western blotting analyses. The results showed that the levels of HSP70 and TNFα were up-regulated under heat conditions (40 °C). HSP70 acted as a chaperone to maintain TNFα homeostasis with rising the temperature, but knockdown of HSP70 could not down-regulate the level of TNFα. Furthermore, TNFα could not influence the expression of HSP70 under normal and heat conditions. BBR targeted both HSP70 and TNFα by suppressing their gene transcription, thereby decreasing body temperature under heat conditions. In conclusion, BBR has a potential to be developed as a therapeutic strategy for suppressing the thermal effects in hot environments.
[Mh] Termos MeSH primário: Berberina/farmacologia
Proteínas de Choque Térmico HSP70/genética
Transtornos de Estresse por Calor/tratamento farmacológico
TATA Box/efeitos dos fármacos
Fator de Necrose Tumoral alfa/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Choque Térmico HSP70/metabolismo
Transtornos de Estresse por Calor/genética
Transtornos de Estresse por Calor/metabolismo
Temperatura Alta
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos ICR
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HSP70 Heat-Shock Proteins); 0 (Tumor Necrosis Factor-alpha); 0I8Y3P32UF (Berberine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170417
[St] Status:MEDLINE


  5 / 3147 MEDLINE  
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[PMID]:28388414
[Au] Autor:Slobodin B; Han R; Calderone V; Vrielink JA; Loayza-Puch F; Elkon R; Agami R
[Ad] Endereço:Division of Oncogenomics, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, the Netherlands. Electronic address: boris.slobodin@gmail.com.
[Ti] Título:Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation.
[So] Source:Cell;169(2):326-337.e12, 2017 Apr 06.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection between transcription and translation. Employing an unbiased screen of multiple human promoters, we identified a positive effect of TATA box on translation and a general coupling between mRNA expression and translational efficiency. Using a CRISPR-Cas9-mediated approach, genome-wide analyses, and in vitro experiments, we show that the rate of transcription regulates the efficiency of translation. Furthermore, we demonstrate that m A modification of mRNAs is co-transcriptional and depends upon the dynamics of the transcribing RNAPII. Suboptimal transcription rates lead to elevated m A content, which may result in reduced translation. This study uncovers a general and widespread link between transcription and translation that is governed by epigenetic modification of mRNAs.
[Mh] Termos MeSH primário: Adenosina/análogos & derivados
Regulação da Expressão Gênica
Biossíntese de Proteínas
Processamento Pós-Transcricional do RNA
RNA Mensageiro/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Adenosina/metabolismo
Seres Humanos
Metilação
Iniciação Traducional da Cadeia Peptídica
RNA Polimerase II/metabolismo
TATA Box
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 1867-73-8 (N(6)-methyladenosine); EC 2.7.7.- (RNA Polymerase II); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE


  6 / 3147 MEDLINE  
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[PMID]:28141828
[Au] Autor:Costa S; Nicosia A; Cuttitta A; Gianguzza F; Ragusa MA
[Ad] Endereço:Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy.
[Ti] Título:An Intronic cis-Regulatory Element Is Crucial for the Alpha Tubulin Pl-Tuba1a Gene Activation in the Ciliary Band and Animal Pole Neurogenic Domains during Sea Urchin Development.
[So] Source:PLoS One;12(1):e0170969, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In sea urchin development, structures derived from neurogenic territory control the swimming and feeding responses of the pluteus as well as the process of metamorphosis. We have previously isolated an alpha tubulin family member of Paracentrotus lividus (Pl-Tuba1a, formerly known as Pl-Talpha2) that is specifically expressed in the ciliary band and animal pole neurogenic domains of the sea urchin embryo. In order to identify cis-regulatory elements controlling its spatio-temporal expression, we conducted gene transfer experiments, transgene deletions and site specific mutagenesis. Thus, a genomic region of about 2.6 Kb of Pl-Tuba1a, containing four Interspecifically Conserved Regions (ICRs), was identified as responsible for proper gene expression. An enhancer role was ascribed to ICR1 and ICR2, while ICR3 exerted a pivotal role in basal expression, restricting Tuba1a expression to the proper territories of the embryo. Additionally, the mutation of the forkhead box consensus sequence binding site in ICR3 prevented Pl-Tuba1a expression.
[Mh] Termos MeSH primário: Cílios/metabolismo
Desenvolvimento Embrionário/genética
Íntrons/genética
Neurogênese/genética
Paracentrotus/genética
Regiões Promotoras Genéticas/genética
Ativação Transcricional/genética
Tubulina (Proteína)/genética
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação/genética
Sequência Conservada/genética
Elementos Facilitadores Genéticos/genética
Regulação da Expressão Gênica no Desenvolvimento
Proteínas de Fluorescência Verde/metabolismo
Modelos Genéticos
Mutagênese Sítio-Dirigida
Paracentrotus/embriologia
Deleção de Sequência/genética
Especificidade da Espécie
TATA Box/genética
Fatores de Transcrição/metabolismo
Sítio de Iniciação de Transcrição
Transgenes
Tubulina (Proteína)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transcription Factors); 0 (Tubulin); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170969


  7 / 3147 MEDLINE  
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[PMID]:28130343
[Au] Autor:Kugel JF; Goodrich JA
[Ad] Endereço:Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA.
[Ti] Título:Finding the start site: redefining the human initiator element.
[So] Source:Genes Dev;31(1):1-2, 2017 01 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transcription by RNA polymerase II (Pol II) is dictated in part by core promoter elements, which are DNA sequences flanking the transcription start site (TSS) that help direct the proper initiation of transcription. Taking advantage of recent advances in genome-wide sequencing approaches, Vo ngoc and colleagues (pp. 6-11) identified transcripts with focused sites of initiation and found that many were transcribed from promoters containing a new consensus sequence for the human initiator (Inr) core promoter element.
[Mh] Termos MeSH primário: Regiões Promotoras Genéticas
Sítio de Iniciação de Transcrição
[Mh] Termos MeSH secundário: Sequência de Bases
Sequência Consenso
Seres Humanos
RNA Polimerase II/genética
TATA Box
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE
[do] DOI:10.1101/gad.295980.117


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[PMID]:28108474
[Au] Autor:Vo Ngoc L; Cassidy CJ; Huang CY; Duttke SH; Kadonaga JT
[Ad] Endereço:Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA.
[Ti] Título:The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters.
[So] Source:Genes Dev;31(1):6-11, 2017 Jan 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analysis of 7678 focused transcription start sites revealed 40% with a perfect match to the Inr and 16% with a single mismatch outside of the CA core. TATA-like sequences are underrepresented in Inr promoters. This consensus is a key component of the DNA sequence rules that specify transcription initiation in humans.
[Mh] Termos MeSH primário: Regiões Promotoras Genéticas/genética
Sítio de Iniciação de Transcrição
[Mh] Termos MeSH secundário: Sequência Conservada/genética
Análise Mutacional de DNA
Seres Humanos
Células MCF-7
Mutação
Homologia de Sequência do Ácido Nucleico
TATA Box/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170122
[St] Status:MEDLINE
[do] DOI:10.1101/gad.293837.116


  9 / 3147 MEDLINE  
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[PMID]:28060464
[Au] Autor:Montes M; Moreira-Ramos S; Rojas DA; Urbina F; Käufer NF; Maldonado E
[Ad] Endereço:Programa Biología Celular y Molecular, Facultad de Medicina, Instituto de Ciencias Biomédicas, Universidad de Chile, Santiago, Chile.
[Ti] Título:RNA polymerase II components and Rrn7 form a preinitiation complex on the HomolD box to promote ribosomal protein gene expression in Schizosaccharomyces pombe.
[So] Source:FEBS J;284(4):615-633, 2017 Feb.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In Schizosaccharomyces pombe, ribosomal protein gene (RPG) promoters contain a TATA box analog, the HomolD box, which is bound by the Rrn7 protein. Despite the importance of ribosome biogenesis for cell survival, the mechanisms underlying RPG transcription remain unknown. In this study, we found that components of the RNA polymerase II (RNAPII) system, consisting of the initiation or general transcription factors (GTFs) TFIIA, IIB, IIE, TATA-binding protein (TBP) and the RNAPII holoenzyme, interacted directly with Rrn7 in vitro, and were able to form a preinitiation complex (PIC) on the HomolD box. PIC complex formation follows an ordered pathway on these promoters. The GTFs and RNAPII can also be cross-linked to HomolD-containing promoters in vivo. In an in vitro reconstituted transcription system, RNAPII components and Rrn7 were necessary for HomolD-directed transcription. The Mediator complex was required for basal transcription from those promoters in whole cell extract (WCE). The Med17 subunit of Mediator also can be cross-linked to the promoter region of HomolD-containing promoters in vivo, suggesting the presence of the Mediator complex on HomolD box-containing promoters. Together, these data show that components of the RNAPII machinery and Rrn7 participate in the PIC assembly on the HomolD box, thereby directing RPG transcription.
[Mh] Termos MeSH primário: Proteínas Fúngicas/genética
Regulação Fúngica da Expressão Gênica
Proteínas Pol1 do Complexo de Iniciação de Transcrição/genética
Proteínas Ribossômicas/genética
Schizosaccharomyces/genética
TATA Box
[Mh] Termos MeSH secundário: Sítios de Ligação
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas Fúngicas/metabolismo
Expressão Gênica
Complexo Mediador/genética
Complexo Mediador/metabolismo
Proteínas Pol1 do Complexo de Iniciação de Transcrição/metabolismo
Regiões Promotoras Genéticas
Ligação Proteica
RNA Polimerase II/genética
RNA Polimerase II/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Ribossômicas/metabolismo
Schizosaccharomyces/metabolismo
Proteína de Ligação a TATA-Box/genética
Proteína de Ligação a TATA-Box/metabolismo
Fator de Transcrição TFIIA/genética
Fator de Transcrição TFIIA/metabolismo
Fator de Transcrição TFIIB/genética
Fator de Transcrição TFIIB/metabolismo
Fatores de Transcrição TFII/genética
Fatores de Transcrição TFII/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Mediator Complex); 0 (Pol1 Transcription Initiation Complex Proteins); 0 (Recombinant Proteins); 0 (Ribosomal Proteins); 0 (TATA-Box Binding Protein); 0 (Transcription Factor TFIIA); 0 (Transcription Factor TFIIB); 0 (Transcription Factors, TFII); 0 (transcription factor TFIIE); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14006


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[PMID]:28049897
[Au] Autor:Kobayashi T
[Ad] Endereço:Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University.
[Ti] Título:Expression and Regulation of Tal2 during Neuronal Differentiation in P19 Cells.
[So] Source:Yakugaku Zasshi;137(1):61-71, 2017.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:T-cell acute lymphocytic leukemia 2 (Tal2) is a gene encoding a member of the basic helix-loop-helix transcription factor family, which is essential for the normal development of the mouse brain. We found that Tal2 was induced during neural differentiation in P19 cells, which are pluripotent mouse embryonal carcinoma cells that differentiate into the neural lineage upon both exposure to all-trans retinoic acid (atRA) and the formation of cell aggregation. Tal2 expression during neural differentiation in P19 cells was detected within 3 h after induction with atRA and retinoic acid receptor α (RARα). The atRA-RARα complex is known to bind to a characteristic retinoic acid response element (RARE) located in the promoter of target genes. We found a RARE-like element in the intron of Tal2. We also found a TATA-box-like element in the 5' region. The TATA-box-like element functioned as a core promoter, and TATA- box binding protein bound to this element upstream of Tal2 in P19 cells. The RARE-like element responded to atRA signaling that activated the transcription, and RARα was bound to this element in the intron of Tal2 in P19 cells. Furthermore, the interaction between these elements on Tal2 was confirmed in a chromatin immunoprecipitation assay. Because the neural differentiation of P19 cells mimics in part the development of the nervous system, P19 cells are useful for studying the mechanism underlying the role of Tal2 in neural differentiation. Further work is underway to clarify the function of Tal2 in neural differentiation using the differentiation system of P19 cells.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/fisiologia
Diferenciação Celular/genética
Regulação da Expressão Gênica no Desenvolvimento/genética
Expressão Gênica/genética
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/fisiologia
Neurônios/citologia
[Mh] Termos MeSH secundário: Animais
Camundongos
Regiões Promotoras Genéticas
Receptor alfa de Ácido Retinoico
TATA Box
Tretinoína/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Neoplasm Proteins); 0 (Retinoic Acid Receptor alpha); 0 (Tal2 protein, mouse); 5688UTC01R (Tretinoin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.16-00176



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