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[PMID]: 29274339 [Au] Autor: Alniss H; Zamiri B; Khalaj M; Pearson CE; Macgregor RB [Ad] Endereço: College of Pharmacy, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirates. [Ti] Título: Thermodynamic and spectroscopic investigations of TMPyP4 association with guanine- and cytosine-rich DNA and RNA repeats of C9orf72. [So] Source: Biochem Biophys Res Commun;495(4):2410-2417, 2018 01 22. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: An expansion of the hexanucleotide repeat (GGGGCC)n·(GGCCCC)n in the C9orf72 promoter has been shown to be the cause of Amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD). The C9orf72 repeat can form four-stranded structures; the cationic porphyrin (TMPyP4) binds and distorts these structures. METHODS: Isothermal titration calorimetry (ITC), and circular dichroism (CD) were used to study the binding of TMPyP4 to the C-rich and G-rich DNA and RNA oligos containing the hexanucleotide repeat at pH 7.5 and 0.1 M K . RESULTS: The CD spectra of G-rich DNA and RNA TMPyP4 complexes showed features of antiparallel and parallel G-quadruplexes, respectively. The shoulder at 260 nm in the CD spectrum becomes more intense upon formation of complexes between TMPyP4 and the C-rich DNA. The peak at 290 nm becomes more intense in the c-rich RNA molecules, suggesting induction of an i-motif structure. The ITC data showed that TMPyP4 binds at two independent sites for all DNA and RNA molecules. CONCLUSIONS: For DNA, the data are consistent with TMPyP4 stacking on the terminal tetrads and intercalation. For RNA, the thermodynamics of the two binding modes are consistent with groove binding and intercalation. In both cases, intercalation is the weaker binding mode. These findings are considered with respect to the structural differences of the folded DNA and RNA molecules and the energetics of the processes that drive site-specific recognition by TMPyP4; these data will be helpful in efforts to optimize the specificity and affinity of the binding of porphyrin-like molecules. [Mh] Termos MeSH primário: Proteína C9orf72/química Proteína C9orf72/genética Citosina/química DNA/química Guanina/química RNA/química Sequências Repetitivas de Ácido Nucleico [Mh] Termos MeSH secundário: Composição de Bases Sítios de Ligação Calorimetria Dicroísmo Circular DNA/genética Ligação Proteica RNA/genética Relação Estrutura-Atividade Termodinâmica [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (C9orf72 Protein); 5Z93L87A1R (Guanine); 63231-63-0 (RNA); 8J337D1HZY (Cytosine); 9007-49-2 (DNA) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171224 [St] Status: MEDLINE

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[PMID]: 29186711 [Au] Autor: Viana PF; Ezaz T; Marajó L; Ferreira M; Zuanon J; Cioffi MB; Bertollo LAC; Gross MC; Feldberg E [Ad] Endereço: Instituto Nacional de Pesquisas da Amazônia, Coordenação de Biodiversidade, Universidade Federal do Amazonas, Manaus, Brazil. [Ti] Título: Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes). [So] Source: Cytogenet Genome Res;153(2):96-104, 2017. [Is] ISSN: 1424-859X [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family. [Mh] Termos MeSH primário: Cromossomos Sexuais/genética Processos de Determinação Sexual Tetraodontiformes/genética [Mh] Termos MeSH secundário: Animais Antígenos Nucleares/genética Brasil Bandeamento Cromossômico DNA Ribossômico/genética Feminino Amplificação de Genes Hibridização in Situ Fluorescente Masculino RNA Ribossômico 18S/genética Sequências Repetitivas de Ácido Nucleico Telômero/genética Telômero/ultraestrutura Tropomiosina/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antigens, Nuclear); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 18S); 0 (Tropomyosin); 0 (nucleolar organizer region associated proteins) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180220 [Lr] Data última revisão: 180220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171130 [St] Status: MEDLINE [do] DOI: 10.1159/000484423

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[PMID]: 28745717 [Au] Autor: Utsunomia R; Scacchetti PC; Hermida M; Fernández-Cebrián R; Taboada X; Fernández C; Bekaert M; Mendes NJ; Robledo D; Mank JE; Taggart JB; Oliveira C; Foresti F; Martínez P [Ad] Endereço: Departamento de Morfologia, Instituto de Biociências, UNESP, Botucatu, Brazil. [Ti] Título: Evolution and conservation of Characidium sex chromosomes. [So] Source: Heredity (Edinb);119(4):237-244, 2017 10. [Is] ISSN: 1365-2540 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Fish species exhibit substantial variation in the degree of genetic differentiation between sex chromosome pairs, and therefore offer the opportunity to study the full range of sex chromosome evolution. We used restriction-site associated DNA sequencing (RAD-seq) to study the sex chromosomes of Characidium gomesi, a species with conspicuous heteromorphic ZW/ZZ sex chromosomes. We screened 9863 single-nucleotide polymorphisms (SNPs), corresponding to ~1 marker/100 kb distributed across the genome for sex-linked variation. With this data set, we identified 26 female-specific RAD loci, putatively located on the W chromosome, as well as 148 sex-associated SNPs showing significant differentiation (average F =0.144) between males and females, and therefore in regions of more recent divergence between the Z and W chromosomes. In addition, we detected 25 RAD loci showing extreme heterozygote deficiency in females but which were in Hardy-Weinberg equilibrium in males, consistent with degeneration of the W chromosome and therefore female hemizygosity. We validated seven female-specific and two sex-associated markers in a larger sample of C. gomesi, of which three localised to the W chromosome, thereby providing useful markers for sexing wild samples. Validated markers were evaluated in other populations and species of the genus Characidium, this exploration suggesting a rapid turnover of W-specific repetitive elements. Together, our analyses point to a complex origin for the sex chromosome of C. gomesi and highlight the utility of RAD-seq for studying the composition and evolution of sex chromosomes systems in wild populations. [Mh] Termos MeSH primário: Caraciformes/genética Evolução Molecular Cromossomos Sexuais/genética [Mh] Termos MeSH secundário: Animais Sequência Conservada/genética Feminino Genoma Masculino Sequências Repetitivas de Ácido Nucleico/genética Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170727 [St] Status: MEDLINE [do] DOI: 10.1038/hdy.2017.43

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[PMID]: 29369562 [Au] Autor: Sulimova GE; Voronkova VN; Perchun AV; Gorlov IF; Randelin AV; Slozhenkina MI; Zlobina EY [Ti] Título: [Characterization of the Russian beef cattle breed gene pools using inter simple sequence repeat DNA analysis (ISSR analysis)]. [So] Source: Genetika;52(9):1081-8, 2016 Sep. [Is] ISSN: 0016-6758 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: The gene pools of beef cattle breeds bred in Russia were characterized on the basis of inter simple sequence repeat DNA analysis (ISSR analysis). Samples of Aberdeen Angus, Kalmyk, and Kazakh Whitehead breeds from Russia, as well as of Hereford breed, hybrids of Kazakh Whitehead and Hereford breeds, and Kazakh Whitehead breed from the Republic of Kazakhstan, were examined. In the examined breeds, 27 AG-ISSR fragments were identified, 25 of which were polymorphic. The examined breeds were different both in the fragment profiles (the presence/absence of individual ISSR fragments) and in their frequencies. It was demonstrated that the hybrid animals lacked some ISSR fragments that were present with high frequencies in parental forms, suggesting considerable genome rearrangement in the hybrid animals (at the regions of microsatellite localization) in crossings of the individuals from different breeds. The level of genetic diversity in Russian beef breeds was consistent with the values typical of farmed populations (breeds). The genetic diversity parameters assessed by applying Nei's gene diversity index and the Shannon index varied from 0.0218 to 0.0605 and from 0.0225 to 0.0819, respectively. The highest Shannon index value was detected in the Kalmyk breed (0.0837) and Kazakh Whitehead breed from Russia (0.0819), and the highest level of Nei's gene diversity index was found in the Kalmyk breed (0.0562) and in both populations of the Kazakh Whitehead breed (0.0509 and 0.0605). The high level of genetic similarity (according to Nei) was revealed between Russian beef cattle breeds and Hereford cattle: 0.839 (for the Kazakh Whitehead breed from Russia) and 0.769 (for the Kalmyk breed). [Mh] Termos MeSH primário: Bovinos/genética Pool Gênico Variação Genética Sequências Repetitivas de Ácido Nucleico [Mh] Termos MeSH secundário: Animais Federação Russa [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180126 [St] Status: MEDLINE

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[PMID]: 28457869 [Au] Autor: Joshi SS; Meller VH [Ad] Endereço: Department of Biological Sciences, Wayne State University, 5047 Gullen Mall, Detroit, MI 48020, USA. [Ti] Título: Satellite Repeats Identify X Chromatin for Dosage Compensation in Drosophila melanogaster Males. [So] Source: Curr Biol;27(10):1393-1402.e2, 2017 May 22. [Is] ISSN: 1879-0445 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A common feature of sex chromosomes is coordinated regulation of X-linked genes in one sex. Drosophila melanogaster males have one X chromosome, whereas females have two. The resulting imbalance in gene dosage is corrected by increased expression from the single X chromosome of males, a process known as dosage compensation. In flies, compensation involves recruitment of the male-specific lethal (MSL) complex to X-linked genes and modification of chromatin to increase expression. The extraordinary selectivity of the MSL complex for the X chromosome has never been explained. We previously demonstrated that the small interfering RNA (siRNA) pathway and siRNA from a family of X-linked satellite repeats (1.688 repeats) promote X recognition. Now, we test the ability of 1.688 DNA to attract compensation to genes nearby and report that autosomal integration of 1.688 repeats increases MSL recruitment and gene expression in surrounding regions. Placement of 1.688 repeats opposite a lethal autosomal deletion achieves partial rescue of males, demonstrating functional compensation of autosomal chromatin. Females block formation of the MSL complex and are not rescued. The 1.688 repeats are therefore cis-acting elements that guide dosage compensation. Furthermore, 1.688 siRNA enhances rescue of males with a lethal deletion but only when repeat DNA is present on the intact homolog. We propose that the siRNA pathway promotes X recognition by enhancing the ability of 1.688 DNA to attract compensation in cis. The dense and near-exclusive distribution of 1.688 sequences along the X chromosome suggests that they play a primary role in determining X identity during dosage compensation. [Mh] Termos MeSH primário: Cromatina DNA Satélite Compensação de Dosagem (Genética) Proteínas de Drosophila/genética Drosophila melanogaster/genética Sequências Repetitivas de Ácido Nucleico Cromossomo X [Mh] Termos MeSH secundário: Animais Drosophila melanogaster/crescimento & desenvolvimento Feminino Regulação da Expressão Gênica Masculino Fatores Sexuais [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Chromatin); 0 (DNA, Satellite); 0 (Drosophila Proteins) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180202 [Lr] Data última revisão: 180202 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170502 [St] Status: MEDLINE

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[PMID]: 29246535 [Au] Autor: Taboada X; Rey M; Bouza C; Viñas A [Ad] Endereço: Departamento de Zoología, Genética y Antropología Física, Facultad de Biología, CIBUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. [Ti] Título: Cytogenomic analysis of several repetitive DNA elements in turbot (Scophthalmus maximus). [So] Source: Gene;644:4-12, 2018 Feb 20. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Repetitive DNA plays a fundamental role in the organization, size and evolution of eukaryotic genomes. The sequencing of the turbot revealed a small and compact genome, as in all flatfish studied to date. The assembly of repetitive regions is still incomplete because it is difficult to correctly identify their position, number and array. The combination of classical cytogenetic techniques along with high quality sequencing is essential to increase the knowledge of the structure and composition of these sequences and, thus, of the structure and function of the whole genome. In this work, the in silico analysis of H1 histone, 5S rDNA, telomeric and Rex repetitive sequences, was compared to their chromosomal mapping by fluorescent in situ hybridization (FISH), providing a more comprehensive picture of these elements in the turbot genome. FISH assays confirmed the location of H1 in LG8; 5S rDNA in LG4 and LG6; telomeric sequences at the end of all chromosomes whereas Rex elements were dispersed along most chromosomes. The discrepancies found between both approaches could be related to the sequencing methodology applied in this species and also to the resolution limitations of the FISH technique. Turbot cytogenomic analyses have proven to add new chromosomal landmarks in the karyotype of this species, representing a powerful tool to investigate targeted genomic sequences or regions in the genetic and physical maps of this species. [Mh] Termos MeSH primário: DNA/genética Linguados/genética Sequências Repetitivas de Ácido Nucleico/genética [Mh] Termos MeSH secundário: Animais Mapeamento Cromossômico/métodos Análise Citogenética/métodos Genoma/genética Hibridização in Situ Fluorescente/métodos Cariótipo RNA Ribossômico 5S/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (RNA, Ribosomal, 5S); 9007-49-2 (DNA) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180119 [Lr] Data última revisão: 180119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171217 [St] Status: MEDLINE

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[PMID]: 28745309 [Au] Autor: Pan XL; Zhang CL; Nakajima C; Fu J; Shao CX; Zhao LN; Cui JY; Jiao N; Fan CL; Suzuki Y; Hattori T; Li D; Ling H [Ad] Endereço: Department of Microbiology, Wu Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin 150081, China. [Ti] Título: A quantitative and efficient approach to select MIRU-VNTR loci based on accumulation of the percentage differences of strains for discriminating divergent Mycobacterium tuberculosis sublineages. [So] Source: Emerg Microbes Infect;6(7):e68, 2017 Jul 26. [Is] ISSN: 2222-1751 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Although several optimal mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) loci have been suggested for genotyping homogenous Mycobacterium tuberculosis, including the Beijing genotype, a more efficient and convenient selection strategy for identifying optimal VNTR loci is needed. Here 281 M. tuberculosis isolates were analyzed. Beijing genotype and non-Beijing genotypes were identified, as well as Beijing sublineages, according to single nucleotide polymorphisms. A total of 22 MIRU-VNTR loci were used for genotyping. To efficiently select optimal MIRU-VNTR loci, we established accumulations of percentage differences (APDs) between the strains among the different genotypes. In addition, we constructed a minimum spanning tree for clustering analysis of the VNTR profiles. Our findings showed that eight MIRU-VNTR loci displayed disparities in h values of ≥0.2 between the Beijing genotype and non-Beijing genotype isolates. To efficiently discriminate Beijing and non-Beijing genotypes, an optimal VNTR set was established by adding loci with APDs ranging from 87.2% to 58.8%, resulting in the construction of a nine-locus set. We also found that QUB11a is a powerful locus for separating ST10s (including ST10, STF and STCH1) and ST22s (including ST22 and ST8) strains, whereas a combination of QUB11a, QUB4156, QUB18, Mtub21 and QUB26 could efficiently discriminate Beijing sublineages. Our findings suggested that two nine-locus sets were not only efficient for distinguishing the Beijing genotype from non-Beijing genotype strains, but were also suitable for sublineage genotyping with different discriminatory powers. These results indicate that APD represents a quantitative and efficient approach for selecting MIRU-VNTR loci to discriminate between divergent M. tuberculosis sublineages. [Mh] Termos MeSH primário: Técnicas de Tipagem Bacteriana/métodos Repetições Minissatélites/genética Mycobacterium tuberculosis/classificação Mycobacterium tuberculosis/genética Sequências Repetitivas de Ácido Nucleico [Mh] Termos MeSH secundário: Pequim DNA Bacteriano Variação Genética Genótipo Seres Humanos Polimorfismo de Nucleotídeo Único Tuberculose/microbiologia Tuberculose/transmissão [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180118 [Lr] Data última revisão: 180118 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170727 [St] Status: MEDLINE [do] DOI: 10.1038/emi.2017.58

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[PMID]: 28464822 [Au] Autor: Conte MA; Gammerdinger WJ; Bartie KL; Penman DJ; Kocher TD [Ad] Endereço: Department of Biology, University of Maryland, 20742, College Park, MD, USA. [Ti] Título: A high quality assembly of the Nile Tilapia (Oreochromis niloticus) genome reveals the structure of two sex determination regions. [So] Source: BMC Genomics;18(1):341, 2017 05 02. [Is] ISSN: 1471-2164 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Tilapias are the second most farmed fishes in the world and a sustainable source of food. Like many other fish, tilapias are sexually dimorphic and sex is a commercially important trait in these fish. In this study, we developed a significantly improved assembly of the tilapia genome using the latest genome sequencing methods and show how it improves the characterization of two sex determination regions in two tilapia species. RESULTS: A homozygous clonal XX female Nile tilapia (Oreochromis niloticus) was sequenced to 44X coverage using Pacific Biosciences (PacBio) SMRT sequencing. Dozens of candidate de novo assemblies were generated and an optimal assembly (contig NG50 of 3.3Mbp) was selected using principal component analysis of likelihood scores calculated from several paired-end sequencing libraries. Comparison of the new assembly to the previous O. niloticus genome assembly reveals that recently duplicated portions of the genome are now well represented. The overall number of genes in the new assembly increased by 27.3%, including a 67% increase in pseudogenes. The new tilapia genome assembly correctly represents two recent vasa gene duplication events that have been verified with BAC sequencing. At total of 146Mbp of additional transposable element sequence are now assembled, a large proportion of which are recent insertions. Large centromeric satellite repeats are assembled and annotated in cichlid fish for the first time. Finally, the new assembly identifies the long-range structure of both a ~9Mbp XY sex determination region on LG1 in O. niloticus, and a ~50Mbp WZ sex determination region on LG3 in the related species O. aureus. CONCLUSIONS: This study highlights the use of long read sequencing to correctly assemble recent duplications and to characterize repeat-filled regions of the genome. The study serves as an example of the need for high quality genome assemblies and provides a framework for identifying sex determining genes in tilapia and related fish species. [Mh] Termos MeSH primário: Ciclídeos/genética Genômica Processos de Determinação Sexual/genética [Mh] Termos MeSH secundário: Animais Loci Gênicos/genética Anotação de Sequência Molecular Sequências Repetitivas de Ácido Nucleico/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 180104 [Lr] Data última revisão: 180104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170504 [St] Status: MEDLINE [do] DOI: 10.1186/s12864-017-3723-5

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[PMID]: 29030254 [Au] Autor: Chen N; Sha LN; Dong ZZ; Tang C; Wang Y; Kang HY; Zhang HQ; Yan XB; Zhou YH; Fan X [Ad] Endereço: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, China. [Ti] Título: Complete structure and variation of the chloroplast genome of Agropyron cristatum (L.) Gaertn. [So] Source: Gene;640:86-96, 2018 Jan 15. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Agropyron cristatum (L.) Gaertner, a perennial grass in the tribe Triticeae (Poaceae), is a wild relative of cereal crops that is suitable for genetic improvement. In this study, we first sequenced the complete chloroplast (cp) genome of Ag. cristatum using Hiseq4000 PE150. The Ag. cristatum chloroplast genome is 135,554bp in length, has a typical quadripartite structure and contains 76 protein-coding genes, 29 tRNA genes and four rRNA genes. The cp genome of Ag. cristatum was used for comparison with other seven Triticeae species. One large variable region (800bp), which primarily contained the rpl23 (non-reciprocally translocated from IRs) and accD genes, was detected between rbcL gene and psaI gene within LSC region. The deletion of the accD and translocated rpl23 genes in Ag. cristatum indicated an independent gene-loss events or an additional divergence in Triticeae. Analyses of the dn/ds ratio and K2-P's genetic distance for 76 protein-coding genes showed that genes with evolutionary divergence might suffer from the effect of sequence regional constraints or gene functional constraints in Triticeae species. Our research will generally contribute to the knowledge of plastid genome evolution in Triticeae. [Mh] Termos MeSH primário: Agropyron/genética DNA de Cloroplastos/genética Genes de Cloroplastos Marcadores Genéticos Variação Genética Genoma de Cloroplastos [Mh] Termos MeSH secundário: Agropyron/crescimento & desenvolvimento Sequência de Bases Evolução Molecular Filogenia Sequências Repetitivas de Ácido Nucleico Análise de Sequência de DNA/métodos Homologia de Sequência [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Chloroplast); 0 (Genetic Markers) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171128 [Lr] Data última revisão: 171128 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171015 [St] Status: MEDLINE

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[PMID]: 28951621 [Au] Autor: Grow EJ [Ad] Endereço: Department of Oncological Sciences, Huntsman Cancer Institute and Howard Hughes Medical Institute, Salt Lake City, Utah, USA. [Ti] Título: A fine LINE-1 in mouse embryonic chromatin regulation. [So] Source: Nat Genet;49(10):1418-1419, 2017 Sep 27. [Is] ISSN: 1546-1718 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The functional role of repetitive elements in mammalian genomes is still largely unexplored. A new study provides evidence that LINE-1 retrotransposons regulate chromatin dynamics and are essential for normal embryonic development in mice. [Mh] Termos MeSH primário: Cromatina Retroelementos [Mh] Termos MeSH secundário: Animais Desenvolvimento Embrionário Genoma Camundongos Sequências Repetitivas de Ácido Nucleico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Chromatin); 0 (Retroelements) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171026 [Lr] Data última revisão: 171026 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170928 [St] Status: MEDLINE [do] DOI: 10.1038/ng.3960

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