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[PMID]:29220431
[Au] Autor:Franco ME; Bitencourt TA; Marins M; Fachin AL
[Ad] Endereço:Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av: Costabile Romano 2201, 14096-900, Ribeirao Preto SP, Brazil.
[Ti] Título:In silico characterization of tandem repeats in Trichophyton rubrum and related dermatophytes provides new insights into their role in pathogenesis.
[So] Source:Database (Oxford);2017, 2017 Jan 01.
[Is] ISSN:1758-0463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Database URL: http://comp.mch.ifsuldeminas.edu.br/dtrdb/.
[Mh] Termos MeSH primário: Arthrodermataceae
DNA Fúngico/genética
Bases de Dados Genéticas
Sequências de Repetição em Tandem/genética
Trichophyton
[Mh] Termos MeSH secundário: Animais
Arthrodermataceae/genética
Arthrodermataceae/patogenicidade
Simulação por Computador
Dermatomicoses/microbiologia
Seres Humanos
Internet
Trichophyton/genética
Trichophyton/patogenicidade
Interface Usuário-Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1093/database/bax035


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[PMID]:28460451
[Au] Autor:Tsitsipatis D; Jayavelu AK; Müller JP; Bauer R; Schmidt-Arras D; Mahboobi S; Schnöder TM; Heidel F; Böhmer FD
[Ad] Endereço:Institute of Molecular Cell Biology, CMB, Jena University Hospital, Jena, Germany.
[Ti] Título:Synergistic killing of FLT3ITD-positive AML cells by combined inhibition of tyrosine-kinase activity and N-glycosylation.
[So] Source:Oncotarget;8(16):26613-26624, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fms-like tyrosine kinase 3 (FLT3) with internal tandem duplications (ITD) is a major oncoprotein in acute myeloid leukemia (AML), and confers an unfavorable prognosis. Interference with FLT3ITD signaling is therefore pursued as a promising therapeutic strategy. In this study we show that abrogation of FLT3ITD glycoprotein maturation using low doses of the N-glycosylation inhibitor tunicamycin has anti-proliferative and pro-apoptotic effects on FLT3ITD-expressing human and murine cell lines. This effect is mediated in part by arresting FLT3ITD in an underglycosylated state and thereby attenuating FLT3ITD-driven AKT and ERK signaling. In addition, tunicamycin caused pronounced endoplasmatic reticulum stress and apoptosis through activation of protein kinase RNA-like endoplasmic reticulum kinase (PERK) and activation of the gene encoding CCAAT-enhancer-binding protein homologous protein (CHOP). PERK inhibition with a small molecule attenuated CHOP induction and partially rescued cells from apoptosis. Combination of tunicamycin with potent FLT3ITD kinase inhibitors caused synergistic cell killing, which was highly selective for cell lines and primary AML cells expressing FLT3ITD. Although tunicamycin is currently not a clinically applicable drug, we propose that mild inhibition of N-glycosylation may have therapeutic potential in combination with FLT3 kinase inhibitors for FLT3ITD-positive AML.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Duplicação Gênica
Leucemia Mieloide Aguda/genética
Inibidores de Proteínas Quinases/farmacologia
Sequências de Repetição em Tandem
Tirosina Quinase 3 Semelhante a fms/genética
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Apoptose/genética
Linhagem Celular Tumoral
Sinergismo Farmacológico
Estresse do Retículo Endoplasmático
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Expressão Gênica
Glicosilação/efeitos dos fármacos
Seres Humanos
Leucemia Mieloide Aguda/tratamento farmacológico
Leucemia Mieloide Aguda/metabolismo
Leucemia Mieloide Aguda/patologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
Células Tumorais Cultivadas
Tunicamicina/farmacologia
Tirosina Quinase 3 Semelhante a fms/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Protein Kinase Inhibitors); 11089-65-9 (Tunicamycin); EC 2.7.10.1 (fms-Like Tyrosine Kinase 3); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15772


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[PMID]:29338045
[Au] Autor:Kim AR; Lee DH; Lee SH; Rubino I; Choi HJ; Quan FS
[Ad] Endereço:Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea.
[Ti] Título:Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein.
[So] Source:PLoS One;13(1):e0191277, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine efficacy in a mouse model. For VLP vaccines, tandem gene (1-780 bp) for V1 VLPs and tandem repeat gene (repeated 450-780 bp) for V5 VLPs were constructed in pFastBacTM vectors, respectively. Influenza matrix protein 1 (M1) was used as a core protein in the VLPs. Notably, upon challenge infection, significantly lower virus loads were measured in the lung of mice immunized with V1 or V5 VLPs compared to those of naïve mice and formalin-inactivated RSV immunized control mice. In particular, V5 VLPs immunization showed significantly lower virus titers than V1 VLPs immunization. Furthermore, V5 VLPs immunization elicited increased memory B cells responses in the spleen. These results indicated that V5 VLP vaccine containing tandem repeat gene protein provided better protection than V1 VLPs with significantly decreased inflammation in the lungs. Thus, V5 VLPs could be a potential vaccine candidate against RSV.
[Mh] Termos MeSH primário: Infecções por Vírus Respiratório Sincicial/prevenção & controle
Vacinas contra Vírus Sincicial Respiratório/farmacologia
Vírus Sincicial Respiratório Humano/genética
Vírus Sincicial Respiratório Humano/imunologia
Vacinas de Partículas Semelhantes a Vírus/farmacologia
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Modelos Animais de Doenças
Feminino
Genes Virais
Seres Humanos
Pulmão/imunologia
Pulmão/patologia
Pulmão/virologia
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Vírus Respiratório Sincicial/imunologia
Infecções por Vírus Respiratório Sincicial/virologia
Vacinas contra Vírus Sincicial Respiratório/genética
Sequências de Repetição em Tandem
Vacinas de Partículas Semelhantes a Vírus/genética
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Respiratory Syncytial Virus Vaccines); 0 (Vaccines, Virus-Like Particle); 0 (Viral Envelope Proteins); 0 (attachment protein G)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191277


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[PMID]:29232688
[Au] Autor:Raskina O
[Ad] Endereço:Institute of Evolution, University of Haifa, Haifa, Israel.
[Ti] Título:Genotype- and Cell-Specific Dynamics of Tandem Repeat Patterns in Aegilops speltoides Tausch (Poaceae, Triticeae).
[So] Source:Cytogenet Genome Res;153(2):105-116, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA. However, despite the large amount of accumulated data, the impact of the complex repetitive DNA fraction on genome reorganization and functioning and the mechanisms balancing and maintaining the structural integrity of the genome are not fully understood. Homologous recombination is thought to play a key role in both genome reshuffling and stabilization, while the contribution of nonhomologous recombination seems to be undervalued. Here, tandem repeat patterns and dynamics during pollen mother cell development were addressed, with a focus on the meiotic recombination that determines chromosome/genome repatterning and stabilization under cross-pollination and artificial hybridization in wild goatgrass, Aegilops speltoides. Native plants from contrasting allopatric populations and artificially created intraspecific hybrids were investigated using a FISH approach. Cytogenetic analysis uncovered a wide spectrum of genotype- and cell-specific chromosomal rearrangements, suggesting intensive repatterning of both parental and hybrid genomes. The data obtained provide evidence that repetitive elements serve as overabundant and ubiquitous resources for maintaining chromosome architecture/genome integrity through homologous and nonhomologous recombination at the intraorganismal level, and genotype-specific repatterning underlies intrapopulation polymorphisms and intraspecific diversification in the wild.
[Mh] Termos MeSH primário: Cromossomos de Plantas/genética
DNA de Plantas/genética
Poaceae/genética
Sequências de Repetição em Tandem/genética
[Mh] Termos MeSH secundário: Cromossomos de Plantas/ultraestrutura
Cruzamentos Genéticos
Genoma de Planta
Genótipo
Recombinação Homóloga
Hibridização Genética
Hibridização in Situ Fluorescente
Meiose
Metagenômica
Polimorfismo Genético
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1159/000484917


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[PMID]:27777503
[Au] Autor:Bowne SJ; Sullivan LS; Wheaton DK; Locke KG; Jones KD; Koboldt DC; Fulton RS; Wilson RK; Blanton SH; Birch DG; Daiger SP
[Ad] Endereço:Human Genetics Center, School of Public Health, The University of Texas Health Science Center (UTHealth), Houston, TX.
[Ti] Título:North Carolina macular dystrophy (MCDR1) caused by a novel tandem duplication of the gene.
[So] Source:Mol Vis;22:1239-1247, 2016.
[Is] ISSN:1090-0535
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To identify the underlying cause of disease in a large family with North Carolina macular dystrophy (NCMD). METHODS: A large four-generation family (RFS355) with an autosomal dominant form of NCMD was ascertained. Family members underwent comprehensive visual function evaluations. Blood or saliva from six affected family members and three unaffected spouses was collected and DNA tested for linkage to the MCDR1 locus on chromosome 6q12. Three affected family members and two unaffected spouses underwent whole exome sequencing (WES) and subsequently, custom capture of the linkage region followed by next-generation sequencing (NGS). Standard PCR and dideoxy sequencing were used to further characterize the mutation. RESULTS: Of the 12 eyes examined in six affected individuals, all but two had Gass grade 3 macular degeneration features. Large central excavation of the retinal and choroid layers, referred to as a macular caldera, was seen in an age-independent manner in the grade 3 eyes. The calderas are unique to affected individuals with MCDR1. Genome-wide linkage mapping and haplotype analysis of markers from the chromosome 6q region were consistent with linkage to the MCDR1 locus. Whole exome sequencing and custom-capture NGS failed to reveal any rare coding variants segregating with the phenotype. Analysis of the custom-capture NGS sequencing data for copy number variants uncovered a tandem duplication of approximately 60 kb on chromosome 6q. This region contains two genes, and . The duplication creates a partial copy of and a complete copy of . The duplication was found in all affected members of the family and is not present in any unaffected members. The duplication was not seen in 200 ethnically matched normal chromosomes. CONCLUSIONS: The cause of disease in the original family with MCDR1 and several others has been recently reported to be dysregulation of the gene, caused by either single base substitutions in a DNase 1 hypersensitive site upstream of the and genes or a tandem duplication of the gene. The duplication found in the RFS355 family is distinct from the previously reported duplication and provides additional support that dysregulation of , not , is the cause of NCMD mapped to the MCDR1 locus.
[Mh] Termos MeSH primário: Distrofias Hereditárias da Córnea/genética
Proteínas do Olho/genética
Histona-Lisina N-Metiltransferase/genética
Mutação
Sequências de Repetição em Tandem/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Criança
Pré-Escolar
Mapeamento Cromossômico
Distrofias Hereditárias da Córnea/diagnóstico
Feminino
Ligação Genética
Seres Humanos
Masculino
Linhagem
Reação em Cadeia da Polimerase
Polimorfismo de Nucleotídeo Único
Tomografia de Coerência Óptica
Acuidade Visual/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Eye Proteins); 0 (Transcription Factors); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:28470536
[Au] Autor:Lagunas-Rangel FA; Chávez-Valencia V
[Ad] Endereço:Graduate Studies Division, Faculty of Biological and Medical Sciences "Dr Ignacio Chávez", Universidad Michoacana de San Nicolás de Hidalgo, Av. Rafael Carrillo W/N, corner with Dr. Salvador González Herrejón, Bosque Cuauhtémoc, 58020, Morelia, Michoacán, Mexico. flagunas@umich.mx.
[Ti] Título:FLT3-ITD and its current role in acute myeloid leukaemia.
[So] Source:Med Oncol;34(6):114, 2017 Jun.
[Is] ISSN:1559-131X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:FMS-like tyrosine kinase 3 (FLT3) is a proto-oncogene involved in crucial steps of haematopoiesis such as proliferation, differentiation and survival. In recent years, FLT3 has been an important marker in different haematological malignancies, highlighting in acute myeloid leukaemia, where FLT3 mutations have been associated with the clinical prognosis, treatment and survival of patients. The most common form of FLT3 mutation is an internal tandem duplication (ITD) that promotes ligand-independent auto-phosphorylation and constitutive activation of the receptor. FLT3-ITD has been strongly associated with a bad prognosis, leukocytosis, high blast counts, increased risk of relapse and shorter overall survival. In order to improve the clinical condition of FLT3-ITD-positive patients, several FLT3 inhibitors have been developed showing variable results. Currently, the main challenges to be overcome are the different forms of resistance to FLT3 inhibitors. Thus, the purpose of this review is to present, in a general way, the current role that FLT3-ITD mutation plays in patients with AML, with a particular emphasis on the molecular mechanisms associated with clinical prognosis, treatment, and survival of patients.
[Mh] Termos MeSH primário: Leucemia Mieloide Aguda
Tirosina Quinase 3 Semelhante a fms
[Mh] Termos MeSH secundário: Duplicação Gênica
Seres Humanos
Mutação
Prognóstico
Sequências de Repetição em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
EC 2.7.10.1 (FLT3 protein, human); EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s12032-017-0970-x


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[PMID]:28460076
[Au] Autor:Rinaldi FC; Doyle LA; Stoddard BL; Bogdanove AJ
[Ad] Endereço:Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA.
[Ti] Título:The effect of increasing numbers of repeats on TAL effector DNA binding specificity.
[So] Source:Nucleic Acids Res;45(11):6960-6970, 2017 Jun 20.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Transcription activator-like effectors (TALEs) recognize their DNA targets via tandem repeats, each specifying a single nucleotide base in a one-to-one sequential arrangement. Due to this modularity and their ability to bind long DNA sequences with high specificity, TALEs have been used in many applications. Contributions of individual repeat-nucleotide associations to affinity and specificity have been characterized. Here, using in vitro binding assays, we examined the relationship between the number of repeats in a TALE and its affinity, for both target and non-target DNA. Each additional repeat provides extra binding energy for the target DNA, with the gain decaying exponentially such that binding energy saturates. Affinity for non-target DNA also increases non-linearly with the number of repeats, but with a slower decay of gain. The difference between the effect of length on affinity for target versus non-target DNA manifests in specificity increasing then diminishing with increasing TALE length, peaking between 15 and 19 repeats. Modeling across different hypothetical saturation levels and rates of gain decay, reflecting different repeat compositions, yielded a similar range of specificity optima. This range encompasses the mean and median length of native TALEs, suggesting that these proteins as a group have evolved for maximum specificity.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Efetores Semelhantes a Ativadores de Transcrição/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/fisiologia
Sequência de Bases
Sítios de Ligação
DNA Bacteriano/química
Ligação Proteica
Sequências de Repetição em Tandem
Termodinâmica
Efetores Semelhantes a Ativadores de Transcrição/fisiologia
Xanthomonas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Transcription Activator-Like Effectors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx342


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[PMID]:27771520
[Au] Autor:Anniballi F; Fillo S; Giordani F; Auricchio B; Tehran DA; di Stefano E; Mandarino G; De Medici D; Lista F
[Ad] Endereço:National Reference Centre for Botulism, Department of Veterinary Public Health and Food Safety. Istituto Superiore di Sanità, 00161 Rome, Italy. Electronic address: fabrizio.anniballi@iss.it.
[Ti] Título:Multiple-locus variable number of tandem repeat analysis as a tool for molecular epidemiology of botulism: The Italian experience.
[So] Source:Infect Genet Evol;46:28-32, 2016 12.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Clostridium botulinum is the bacterial agent of botulism, a rare but severe neuro-paralytic disease. Because of its high impact, in Italy botulism is monitored by an ad hoc surveillance system. The National Reference Centre for Botulism, as part of this system, collects and analyzes all demographic, epidemiologic, microbiological, and molecular data recovered during cases and/or outbreaks occurred in Italy. A panel of 312 C. botulinum strains belonging to group I were submitted to MLVA sub-typing. Strains, isolated from clinical specimens, food and environmental samples collected during the surveillance activities, were representative of all forms of botulism from all Italian regions. Through clustering analysis isolates were grouped into 12 main clusters. No regional or temporal clustering was detected, demonstrating the high heterogeneity of strains circulating in Italy. This study confirmed that MLVA is capable of sub-typing C. botulinum strains. Moreover, MLVA is effective at tracing and tracking the source of contamination and is helpful for the surveillance system in terms of planning and upgrading of procedures, activities and data collection forms.
[Mh] Termos MeSH primário: Botulismo/microbiologia
Clostridium botulinum/genética
Epidemiologia Molecular/métodos
Sequências de Repetição em Tandem/genética
[Mh] Termos MeSH secundário: Botulismo/epidemiologia
Análise por Conglomerados
Seres Humanos
Itália/epidemiologia
Tipagem de Sequências Multilocus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171210
[Lr] Data última revisão:
171210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:28930470
[Au] Autor:Serrano S; Huarte N; Rujas E; Andreu D; Nieva JL; Jiménez MA
[Ad] Endereço:Institute of Physical Chemistry "Rocasolano" (IQFR-CSIC) , Serrano 119, E-28006 Madrid, Spain.
[Ti] Título:Structure-Related Roles for the Conservation of the HIV-1 Fusion Peptide Sequence Revealed by Nuclear Magnetic Resonance.
[So] Source:Biochemistry;56(41):5503-5511, 2017 Oct 17.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely conserved suggests that high hydrophobicity may not suffice to unleash FP function. Here, we have compared the nuclear magnetic resonance (NMR) structures adopted in nonpolar media by two FP surrogates, wtFP-tag and scrFP-tag, which had equal hydrophobicity but contained wild-type and scrambled core sequences LFLGFLG and FGLLGFL, respectively. In addition, these peptides were tagged at their C-termini with an epitope sequence that folded independently, thereby allowing Western blot detection without interfering with FP structure. We observed similar α-helical FP conformations for both specimens dissolved in the low-polarity medium 25% (v/v) 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), but important differences in contact with micelles of the membrane mimetic dodecylphosphocholine (DPC). Thus, whereas wtFP-tag preserved a helix displaying a Gly-rich ridge, the scrambled sequence lost in great part the helical structure upon being solubilized in DPC. Western blot analyses further revealed the capacity of wtFP-tag to assemble trimers in membranes, whereas membrane oligomers were not observed in the case of the scrFP-tag sequence. We conclude that, beyond hydrophobicity, preserving sequence order is an important feature for defining the secondary structures and oligomeric states adopted by the HIV FP in membranes.
[Mh] Termos MeSH primário: Proteína gp41 do Envelope de HIV/metabolismo
Modelos Moleculares
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência Conservada
Cristalografia por Raios X
Bases de Dados de Proteínas
Epitopos
Proteína gp41 do Envelope de HIV/química
Proteína gp41 do Envelope de HIV/genética
Interações Hidrofóbicas e Hidrofílicas
Micelas
Ressonância Magnética Nuclear Biomolecular
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Fosforilcolina/análogos & derivados
Fosforilcolina/química
Fosforilcolina/metabolismo
Conformação Proteica
Conformação Proteica em alfa-Hélice
Multimerização Proteica
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Técnicas de Síntese em Fase Sólida
Sequências de Repetição em Tandem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epitopes); 0 (HIV Envelope Protein gp41); 0 (Micelles); 0 (Peptide Fragments); 0 (Recombinant Proteins); 0 (gp41 protein, Human immunodeficiency virus 1); 107-73-3 (Phosphorylcholine); 53949-18-1 (dodecylphosphocholine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00745


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[PMID]:28911045
[Au] Autor:Chowdhary A; Sharma C; Meis JF
[Ad] Endereço:Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, India.
[Ti] Título:Azole-Resistant Aspergillosis: Epidemiology, Molecular Mechanisms, and Treatment.
[So] Source:J Infect Dis;216(suppl_3):S436-S444, 2017 Aug 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aspergillus fumigatus remains the most common species in all pulmonary syndromes, followed by Aspergillus flavus which is a common cause of allergic rhinosinusitis, postoperative aspergillosis and fungal keratitis. The manifestations of Aspergillus infections include invasive aspergillosis, chronic pulmonary aspergillosis and bronchitis. Allergic manifestations of inhaled Aspergillus include allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitization. Triazoles are the mainstay of therapy against Aspergillus infections for treatment and prophylaxis. Lately, increased azole resistance in A. fumigatus has become a significant challenge in effective management of aspergillosis. Earlier studies have brought to light the contribution of non-cyp51 mutations along with alterations in cyp51A gene resulting in azole-resistant phenotypes of A. fumigatus. This review highlights the magnitude of azole-resistant aspergillosis and resistance mechanisms implicated in the development of azole-resistant A. fumigatus and address the therapeutic options available.
[Mh] Termos MeSH primário: Aspergilose Broncopulmonar Alérgica/tratamento farmacológico
Aspergillus fumigatus/efeitos dos fármacos
Bronquite/dietoterapia
Farmacorresistência Fúngica
Aspergilose Pulmonar Invasiva/tratamento farmacológico
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/farmacologia
Aspergilose Broncopulmonar Alérgica/diagnóstico
Aspergilose Broncopulmonar Alérgica/epidemiologia
Bronquite/diagnóstico
Bronquite/epidemiologia
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Seres Humanos
Aspergilose Pulmonar Invasiva/diagnóstico
Aspergilose Pulmonar Invasiva/epidemiologia
Mutação Puntual
Sequências de Repetição em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fungal Proteins); 0 (Triazoles); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.14.- (cytochrome P-450 CYP51A, Aspergillus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix210



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