Base de dados : MEDLINE
Pesquisa : G02.111.619 [Categoria DeCS]
Referências encontradas : 1332 [refinar]
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  1 / 1332 MEDLINE  
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[PMID]:24162665
[Au] Autor:Wang X; Son A
[Ad] Endereço:Department of Civil Engineering, Auburn University, Auburn, AL 36849, USA.
[Ti] Título:Effects of pretreatment on the denaturation and fragmentation of genomic DNA for DNA hybridization.
[So] Source:Environ Sci Process Impacts;15(12):2204-12, 2013 Dec.
[Is] ISSN:2050-7895
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DNA hybridization is an important step for a number of bioassays such as fluorescence in situ hybridization, microarrays, as well as the NanoGene assay. Denaturation and fragmentation of genomic DNA are two critical pretreatments for DNA hybridization. However, no thorough and systematic characterization on denaturation and fragmentation has been carried out for the NanoGene assay so far. In this study, we investigated the denaturation and fragmentation of the bacterial gDNA with physical treatments (i.e., heating and sonication) and chemical treatments (i.e., dimethyl sulfoxide). First of all, a simple approach for indicating the denaturation fraction was developed based on the absorbance difference (i.e., hyperchromic effect) between the double-stranded DNA and single-stranded DNA fragments. Then the denaturation capabilities of the treatments to the gDNA were elucidated, followed by the examination of the possible renaturation over time. The fragmentation of the gDNA by each treatment was also investigated. Based on denaturation efficiency, minimum renaturation tendency, and fragmentation, the sonication method was found to be the best among the six methods. We further demonstrated that the sonication method produced the best result among the treatments examined for the DNA hybridization in the NanoGene assay.
[Mh] Termos MeSH primário: DNA/química
Desnaturação de Ácido Nucleico
[Mh] Termos MeSH secundário: Fragmentação do DNA
Genômica
Hibridização de Ácido Nucleico
Renaturação de Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:131122
[Lr] Data última revisão:
131122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131029
[St] Status:MEDLINE
[do] DOI:10.1039/c3em00457k


  2 / 1332 MEDLINE  
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[PMID]:23410932
[Au] Autor:Siddiqui S; Khan I; Zarina S; Ali S
[Ad] Endereço:Department of Biological and Biomedical Sciences, The Aga Khan University, Karachi, Pakistan.
[Ti] Título:Use of the SYBR Green dye for measuring helicase activity.
[So] Source:Enzyme Microb Technol;52(3):196-8, 2013 Mar 05.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we describe a non-radioactive assay that exploits the fluorescent dye SYBR Green to measure the helicase enzyme activity. SYBR Green I emits fluorescence upon intercalation with double-stranded DNA or RNA. The fluorescence is lost proportionally as the nucleic acid is converted to single strands by a helicase, and this decrease in fluorescence intensity can be used to measure the activity of the helicase enzyme. The reaction was prepared by mixing a double-stranded substrate with the helicase enzyme, buffer, ATP and SYBR Green I. After completion, the reaction was terminated by EDTA and fluorescence was measured. Using this technique, a linear increase in substrate release was observed with increasing time and helicase concentrations. The assay described here is speedy, efficient and economical; it holds promise for use in large-scale screening of drugs that target helicases.
[Mh] Termos MeSH primário: Antígenos Transformantes de Poliomavirus/análise
DNA Helicases/análise
Corantes Fluorescentes/análise
Fluorometria/métodos
Compostos Orgânicos/análise
Proteínas não Estruturais Virais/análise
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Antígenos Transformantes de Poliomavirus/metabolismo
Ciprofloxacino/farmacologia
DNA Helicases/metabolismo
Hepacivirus/enzimologia
Renaturação de Ácido Nucleico
Oligonucleotídeos/metabolismo
Vírus 40 dos Símios/enzimologia
Proteínas não Estruturais Virais/antagonistas & inibidores
Proteínas não Estruturais Virais/metabolismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Polyomavirus Transforming); 0 (Fluorescent Dyes); 0 (NS3 protein, hepatitis C virus); 0 (Oligonucleotides); 0 (Organic Chemicals); 0 (Viral Nonstructural Proteins); 163795-75-3 (SYBR Green I); 5E8K9I0O4U (Ciprofloxacin); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.4.- (DNA Helicases)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130216
[St] Status:MEDLINE


  3 / 1332 MEDLINE  
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[PMID]:23226421
[Au] Autor:Hämmerle H; Beich-Frandsen M; Vecerek B; Rajkowitsch L; Carugo O; Djinovic-Carugo K; Bläsi U
[Ad] Endereço:Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
[Ti] Título:Structural and biochemical studies on ATP binding and hydrolysis by the Escherichia coli RNA chaperone Hfq.
[So] Source:PLoS One;7(11):e50892, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Escherichia coli the RNA chaperone Hfq is involved in riboregulation by assisting base-pairing between small regulatory RNAs (sRNAs) and mRNA targets. Several structural and biochemical studies revealed RNA binding sites on either surface of the donut shaped Hfq-hexamer. Whereas sRNAs are believed to contact preferentially the YKH motifs present on the proximal site, poly(A)(15) and ADP were shown to bind to tripartite binding motifs (ARE) circularly positioned on the distal site. Hfq has been reported to bind and to hydrolyze ATP. Here, we present the crystal structure of a C-terminally truncated variant of E. coli Hfq (Hfq(65)) in complex with ATP, showing that it binds to the distal R-sites. In addition, we revisited the reported ATPase activity of full length Hfq purified to homogeneity. At variance with previous reports, no ATPase activity was observed for Hfq. In addition, FRET assays neither indicated an impact of ATP on annealing of two model oligoribonucleotides nor did the presence of ATP induce strand displacement. Moreover, ATP did not lead to destabilization of binary and ternary Hfq-RNA complexes, unless a vast stoichiometric excess of ATP was used. Taken together, these studies strongly suggest that ATP is dispensable for and does not interfere with Hfq-mediated RNA transactions.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Proteínas de Escherichia coli/química
Proteínas de Escherichia coli/metabolismo
Escherichia coli/metabolismo
Fator Proteico 1 do Hospedeiro/química
Fator Proteico 1 do Hospedeiro/metabolismo
Chaperonas Moleculares/química
Chaperonas Moleculares/metabolismo
RNA Bacteriano/metabolismo
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/metabolismo
Sítios de Ligação
Cristalografia por Raios X
Hidrólise
Ligantes
Modelos Moleculares
Renaturação de Ácido Nucleico
Subunidades Proteicas/química
Subunidades Proteicas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Hfq protein, E coli); 0 (Host Factor 1 Protein); 0 (Ligands); 0 (Molecular Chaperones); 0 (Protein Subunits); 0 (RNA, Bacterial); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121211
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0050892


  4 / 1332 MEDLINE  
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[PMID]:22808280
[Au] Autor:Toju H; Tanabe AS; Yamamoto S; Sato H
[Ad] Endereço:The Hakubi Center for Advanced Research, Kyoto University, Sakyo, Kyoto, Japan. toju.hirokazu.4c@kyoto-u.ac.jp
[Ti] Título:High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples.
[So] Source:PLoS One;7(7):e40863, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decomposers, mutualists, and parasites in every biome on the earth. To comprehensively understand the diversity and ecology of this huge kingdom, DNA barcoding targeting the internal transcribed spacer (ITS) region of the nuclear ribosomal repeat has been regarded as a prerequisite procedure. By extensively surveying ITS sequences in public databases, we designed new ITS primers with improved coverage across diverse taxonomic groups of fungi compared to existing primers. An in silico analysis based on public sequence databases indicated that the newly designed primers matched 99% of ascomycete and basidiomycete ITS taxa (species, subspecies or varieties), causing little taxonomic bias toward either fungal group. Two of the newly designed primers could inhibit the amplification of plant sequences and would enable the selective investigation of fungal communities in mycorrhizal associations, soil, and other types of environmental samples. Optimal PCR conditions for the primers were explored in an in vitro investigation. The new primers developed in this study will provide a basis for ecological studies on the diversity and community structures of fungi in the era of massive DNA sequencing.
[Mh] Termos MeSH primário: Ascomicetos/classificação
Ascomicetos/genética
Basidiomycota/classificação
Basidiomycota/genética
Primers do DNA/metabolismo
DNA Fúngico/genética
DNA Intergênico/genética
[Mh] Termos MeSH secundário: Pareamento Incorreto de Bases
Sequência de Bases
Núcleo Celular/genética
Biologia Computacional
Microbiologia Ambiental
Técnicas de Tipagem Micológica
Renaturação de Ácido Nucleico/genética
Plantas/genética
Reação em Cadeia da Polimerase
RNA Ribossômico/genética
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Fungal); 0 (DNA, Intergenic); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120719
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0040863


  5 / 1332 MEDLINE  
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[PMID]:22246766
[Au] Autor:Meselson M
[Ad] Endereço:Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. msm@wjh.harvard.edu
[Ti] Título:Retrospective. Paul Mead Doty (1920-2011).
[So] Source:Science;335(6065):181, 2012 Jan 13.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH secundário: Química/história
DNA/química
História do Século XX
História do Século XXI
Internacionalidade
Biologia Molecular/história
Conformação de Ácido Nucleico
Renaturação de Ácido Nucleico
Estados Unidos
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:Doty PM
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:120116
[Lr] Data última revisão:
120116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120117
[St] Status:MEDLINE
[do] DOI:10.1126/science.1218031


  6 / 1332 MEDLINE  
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[PMID]:22242135
[Au] Autor:Schütze T; Wilhelm B; Greiner N; Braun H; Peter F; Mörl M; Erdmann VA; Lehrach H; Konthur Z; Menger M; Arndt PF; Glökler J
[Ad] Endereço:Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany.
[Ti] Título:Probing the SELEX process with next-generation sequencing.
[So] Source:PLoS One;6(12):e29604, 2011.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: SELEX is an iterative process in which highly diverse synthetic nucleic acid libraries are selected over many rounds to finally identify aptamers with desired properties. However, little is understood as how binders are enriched during the selection course. Next-generation sequencing offers the opportunity to open the black box and observe a large part of the population dynamics during the selection process. METHODOLOGY: We have performed a semi-automated SELEX procedure on the model target streptavidin starting with a synthetic DNA oligonucleotide library and compared results obtained by the conventional analysis via cloning and Sanger sequencing with next-generation sequencing. In order to follow the population dynamics during the selection, pools from all selection rounds were barcoded and sequenced in parallel. CONCLUSIONS: High affinity aptamers can be readily identified simply by copy number enrichment in the first selection rounds. Based on our results, we suggest a new selection scheme that avoids a high number of iterative selection rounds while reducing time, PCR bias, and artifacts.
[Mh] Termos MeSH primário: Técnica de Seleção de Aptâmeros/métodos
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Aptâmeros de Nucleotídeos/genética
Sequência de Bases
Corantes Fluorescentes/metabolismo
Dados de Sequência Molecular
Renaturação de Ácido Nucleico/genética
Oligonucleotídeos/genética
Ligação Proteica
Estreptavidina/metabolismo
Ressonância de Plasmônio de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aptamers, Nucleotide); 0 (Fluorescent Dyes); 0 (Oligonucleotides); 9013-20-1 (Streptavidin)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:150128
[Lr] Data última revisão:
150128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120114
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0029604


  7 / 1332 MEDLINE  
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[PMID]:22092240
[Au] Autor:Fu XH; Wang L; Li MN; Zeng XF; Le YQ
[Ad] Endereço:State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
[Ti] Título:Potential ecological risks of thermal-treated waste recombination DNA discharged into an aquatic environment.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;46(14):1640-7, 2011.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It has been shown that thermal-treatment at 100 ° C can denature deoxyribonucleic acid (DNA), yet this does not cause it to break down completely. To clarify the risk of gene pollution from thermal-treated recombinant DNA, the renaturation characteristics of thermal-denatured plasmid pET-28b and its persistence in aquatic environments were investigated. The results revealed that the double-stranded structure and transforming activity of the thermal-treated plasmid DNA could be recovered even if the thermal-treatment was conducted at 120 ° C. The presence of sodium chloride (NaCl) and ethylenediamine tetraacetic acid (EDTA) led to the increase of renaturation efficiency of the denatured DNA. When thermal-treated plasmid DNA was discharged into simulated aquatic environments with pH values from 5 to 9, it showed a longer persistence at pH 7 and 8 than that at 5, 6 and 9; however, the denatured plasmid DNA could persist for more than 33 min at any pH. Moreover, a higher ionic strength further protected the thermal-denatured plasmids from degradation in the simulated aquatic environment. These results indicated that when the thermal-treated DNA was discharged into an aquatic environment, it might not break down completely in a short period. Therefore, there is the potential for the discarded DNA to renature and transform, which might result in gene pollution.
[Mh] Termos MeSH primário: DNA/química
Plasmídeos/química
Poluentes da Água/química
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Ácido Edético/química
Meio Ambiente
Temperatura Alta
Concentração de Íons de Hidrogênio
Desnaturação de Ácido Nucleico
Renaturação de Ácido Nucleico
Reação em Cadeia da Polimerase
Cloreto de Sódio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Water Pollutants); 451W47IQ8X (Sodium Chloride); 9007-49-2 (DNA); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:160526
[Lr] Data última revisão:
160526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111119
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2011.623940


  8 / 1332 MEDLINE  
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[PMID]:21561110
[Au] Autor:Wang YC; Lin CB; Su JJ; Ru YM; Wu Q; Chen ZB; Mao BW; Tian ZW
[Ad] Endereço:State Key Laboratory for Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
[Ti] Título:Electrochemically-driven large amplitude pH cycling for acid-base driven DNA denaturation and renaturation.
[So] Source:Anal Chem;83(12):4930-5, 2011 Jun 15.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this paper, we present an electrochemically driven large amplitude pH alteration method based on a serial electrolytic cell involving a hydrogen permeable bifacial working electrode such as Pd thin foil. The method allows solution pH to be changed periodically up to ±4~5 units without additional alteration of concentration and/or composition of the system. Application to the acid-base driven cyclic denaturation and renaturation of 290 bp DNA fragments is successfully demonstrated with in situ real-time UV spectroscopic characterization. Electrophoretic analysis confirms that the denaturation and renaturation processes are reversible without degradation of the DNA. The serial electrolytic cell based electrochemical pH alteration method presented in this work would promote investigations of a wide variety of potential-dependent processes and techniques.
[Mh] Termos MeSH primário: Ácidos/química
DNA/química
Técnicas Eletroquímicas/métodos
[Mh] Termos MeSH secundário: Biocatálise
Eletrodos
Enzimas/metabolismo
Concentração de Íons de Hidrogênio
Desnaturação de Ácido Nucleico
Renaturação de Ácido Nucleico
Paládio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acids); 0 (Enzymes); 5TWQ1V240M (Palladium); 9007-49-2 (DNA)
[Em] Mês de entrada:1111
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110513
[St] Status:MEDLINE
[do] DOI:10.1021/ac200656u


  9 / 1332 MEDLINE  
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[PMID]:19565914
[Au] Autor:Bates PJ; Choi EW; Nayak LV
[Ad] Endereço:University of Louisville, Louisville, KY, USA.
[Ti] Título:G-rich oligonucleotides for cancer treatment.
[So] Source:Methods Mol Biol;542:379-92, 2009.
[Is] ISSN:1064-3745
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oligonucleotides with guanosine-rich (G-rich) sequences often have unusual physical and biological properties, including resistance to nucleases, enhanced cellular uptake, and high affinity for particular proteins. Furthermore, we have found that certain G-rich oligonucleotides (GROs) have antiproliferative activity against a range of cancer cells, while having minimal toxic effects on normal cells. We have investigated the mechanism of this activity and studied the relationship between oligonucleotide structural features and biological activity. Our results indicate that the antiproliferative effects of GROs depend on two properties: the ability to form quadruplex structures stabilized by G-quartets and binding affinity for nucleolin protein. Thus, it appears that the antiproliferative GROs are acting as nucleolin aptamers. Because nucleolin is expressed at high levels on the surface of cancer cells, where it mediates the endocytosis of various ligands, it seems likely that nucleolin-dependent uptake of GROs plays a role in their activity. One of the GROs that we have developed, a 26-nucleotide phosphodiester oligodeoxynucleotide now named AS1411 (formerly AGRO100 or GRO26B-OH), is currently being tested as an anticancer agent in Phase II clinical trials.
[Mh] Termos MeSH primário: Guanosina/metabolismo
Biologia Molecular/métodos
Neoplasias/terapia
Oligonucleotídeos/farmacologia
[Mh] Termos MeSH secundário: Sobrevivência Celular/efeitos dos fármacos
Dicroísmo Circular
Desoxirribonucleases/metabolismo
Ensaio de Desvio de Mobilidade Eletroforética
Células HeLa
Seres Humanos
Desnaturação de Ácido Nucleico/efeitos dos fármacos
Renaturação de Ácido Nucleico/efeitos dos fármacos
Oligonucleotídeos/análise
Radioisótopos
Coloração e Rotulagem
Esterilização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Oligonucleotides); 0 (Radioisotopes); 12133JR80S (Guanosine); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:0907
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090702
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-59745-561-9_21


  10 / 1332 MEDLINE  
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[PMID]:19254530
[Au] Autor:Sambriski EJ; Schwartz DC; de Pablo JJ
[Ad] Endereço:Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
[Ti] Título:A mesoscale model of DNA and its renaturation.
[So] Source:Biophys J;96(5):1675-90, 2009 Mar 04.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A mesoscale model of DNA is presented (3SPN.1), extending the scheme previously developed by our group. Each nucleotide is mapped onto three interaction sites. Solvent is accounted for implicitly through a medium-effective dielectric constant and electrostatic interactions are treated at the level of Debye-Hückel theory. The force field includes a weak, solvent-induced attraction, which helps mediate the renaturation of DNA. Model parameterization is accomplished through replica exchange molecular dynamics simulations of short oligonucleotide sequences over a range of composition and chain length. The model describes the melting temperature of DNA as a function of composition as well as ionic strength, and is consistent with heat capacity profiles from experiments. The dependence of persistence length on ionic strength is also captured by the force field. The proposed model is used to examine the renaturation of DNA. It is found that a typical renaturation event occurs through a nucleation step, whereby an interplay between repulsive electrostatic interactions and colloidal-like attractions allows the system to undergo a series of rearrangements before complete molecular reassociation occurs.
[Mh] Termos MeSH primário: DNA/química
Modelos Químicos
Renaturação de Ácido Nucleico
[Mh] Termos MeSH secundário: Algoritmos
Simulação por Computador
Temperatura Alta
Modelos Moleculares
Concentração Osmolar
Eletricidade Estática
Temperatura de Transição
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:0905
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090304
[St] Status:MEDLINE
[do] DOI:10.1016/j.bpj.2008.09.061



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