Base de dados : MEDLINE
Pesquisa : G02.111.669 [Categoria DeCS]
Referências encontradas : 347 [refinar]
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[PMID]:29431330
[Au] Autor:Deynego VN; Elizarov VB; Kaptsov VA
[Ti] Título:[Hygienic and environmental problems of energy-saving illumination in urbanization of Crimea].
[So] Source:Gig Sanit;95(10):909-13, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The article considers the problems offloodlights pollution in the territory of Crimea due to the work of illumination led equipment of the key elements of the international transport artery "China-Europe". There was performed a qualitative assessment of characteristics of led floodlights pollution on the example of the sea surface of the transport crossing through the Kerch Strait. Ichthyologists and oceanographers were shown to estimate the amount of phytoplankton biomass based on sunlight illumination. The excess dose of blue light in the spectrum of led lighting was established to have an impact on phytoplankton greater than solar and lunar light, creating preconditions for the increase of biological mass of phytoplankton and consequently to the formation of the "stern stock". Arising from additional phytoplankton biomass can significantly influence on the schedule offish migration in waters of the Kerch Strait, the biomass of mosquitoes and midges, which are prey for amphibians and birds. The decline of the both light pollution and its negative impact on fauna andflora requires the development of semiconductor sources of white light with a biologically adequate spectrum in the framework of the "Lighting of the lighting equipment of Crimea".
[Mh] Termos MeSH primário: Organismos Aquáticos
Poluição Ambiental
Luz/efeitos adversos
Iluminação
Processos Fototróficos/efeitos da radiação
Urbanização
[Mh] Termos MeSH secundário: Organismos Aquáticos/fisiologia
Organismos Aquáticos/efeitos da radiação
Poluição Ambiental/efeitos adversos
Poluição Ambiental/análise
Poluição Ambiental/prevenção & controle
Seres Humanos
Iluminação/efeitos adversos
Iluminação/métodos
Iluminação/normas
Fotometria/métodos
Fotometria/normas
Fitoplâncton/fisiologia
Fitoplâncton/efeitos da radiação
Saúde Pública/métodos
Saúde Pública/normas
Federação Russa/epidemiologia
Mudança Social
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:28470424
[Au] Autor:Hong X; Chen Z; Zhao C; Yang S
[Ad] Endereço:Department of Bioengineering and Biotechnology, Huaqiao University, No. 668 Jimei Ave, Xiamen, 361021, People's Republic of China.
[Ti] Título:Nitrogen transformation under different dissolved oxygen levels by the anoxygenic phototrophic bacterium Marichromatium gracile.
[So] Source:World J Microbiol Biotechnol;33(6):113, 2017 Jun.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Marichromatium gracile: YL28 (M. gracile YL28) is an anoxygenic phototrophic bacterial strain that utilizes ammonia, nitrate, or nitrite as its sole nitrogen source during growth. In this study, we investigated the removal and transformation of ammonium, nitrate, and nitrite by M. gracile YL28 grown in a combinatorial culture system of sodium acetate-ammonium, sodium acetate-nitrate and sodium acetate-nitrite in response to different initial dissolved oxygen (DO) levels. In the sodium acetate-ammonium system under aerobic conditions (initial DO = 7.20-7.25 mg/L), we detected a continuous accumulation of nitrate and nitrite. However, under semi-anaerobic conditions (initial DO = 4.08-4.26 mg/L), we observed a temporary accumulation of nitrate and nitrite. Interestingly, under anaerobic conditions (initial DO = 0.36-0.67 mg/L), there was little accumulation of nitrate and nitrite, but an increase in nitrous oxide production. In the sodium acetate-nitrite system, nitrite levels declined slightly under aerobic conditions, and nitrite was completely removed under semi-anaerobic and anaerobic conditions. In addition, M. gracile YL28 was able to grow using nitrite as the sole nitrogen source in situations when nitrogen gas produced by denitrification was eliminated. Taken together, the data indicate that M. gracile YL28 performs simultaneous heterotrophic nitrification and denitrification at low-DO levels and uses nitrite as the sole nitrogen source for growth. Our study is the first to demonstrate that anoxygenic phototrophic bacteria perform heterotrophic ammonia-oxidization and denitrification under anaerobic conditions.
[Mh] Termos MeSH primário: Anaerobiose/fisiologia
Chromatiaceae/metabolismo
Nitrogênio/metabolismo
Oxigênio/metabolismo
Processos Fototróficos/fisiologia
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Aerobiose/fisiologia
Amônia/metabolismo
Compostos de Amônio/metabolismo
Bactérias
Chromatiaceae/crescimento & desenvolvimento
Desnitrificação
Processos Heterotróficos/fisiologia
Cinética
Nitratos/metabolismo
Nitrificação
Nitritos/metabolismo
Óxido Nitroso/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Ammonium Compounds); 0 (Nitrates); 0 (Nitrites); 7664-41-7 (Ammonia); K50XQU1029 (Nitrous Oxide); N762921K75 (Nitrogen); RRE756S6Q2 (ammonium acetate); S88TT14065 (Oxygen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2280-z


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[PMID]:28985404
[Au] Autor:Cavaiuolo M; Kuras R; Wollman FA; Choquet Y; Vallon O
[Ad] Endereço:Unité Mixte de Recherche 7141, CNRS/UPMC, Institut de Biologie Physico-Chimique, F-75005 Paris, France.
[Ti] Título:Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.
[So] Source:Nucleic Acids Res;45(18):10783-10799, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In Chlamydomonas reinhardtii, regulation of chloroplast gene expression is mainly post-transcriptional. It requires nucleus-encoded trans-acting protein factors for maturation/stabilization (M factors) or translation (T factors) of specific target mRNAs. We used long- and small-RNA sequencing to generate a detailed map of the transcriptome. Clusters of sRNAs marked the 5' end of all mature mRNAs. Their absence in M-factor mutants reflects the protection of transcript 5' end by the cognate factor. Enzymatic removal of 5'-triphosphates allowed identifying those cosRNA that mark a transcription start site. We detected another class of sRNAs derived from low abundance transcripts, antisense to mRNAs. The formation of antisense sRNAs required the presence of the complementary mRNA and was stimulated when translation was inhibited by chloramphenicol or lincomycin. We propose that they derive from degradation of double-stranded RNAs generated by pairing of antisense and sense transcripts, a process normally hindered by the traveling of the ribosomes. In addition, chloramphenicol treatment, by freezing ribosomes on the mRNA, caused the accumulation of 32-34 nt ribosome-protected fragments. Using this 'in vivo ribosome footprinting', we identified the function and molecular target of two candidate trans-acting factors.
[Mh] Termos MeSH primário: Chlamydomonas reinhardtii/genética
RNA de Cloroplastos/metabolismo
Pequeno RNA não Traduzido/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Chlamydomonas reinhardtii/crescimento & desenvolvimento
Chlamydomonas reinhardtii/metabolismo
Proteínas de Cloroplastos/metabolismo
Perfilação da Expressão Gênica
Inibidores da Síntese de Ácido Nucleico/farmacologia
Processos Fototróficos
Proteínas de Plantas/metabolismo
Biossíntese de Proteínas
RNA Antissenso/metabolismo
RNA Mensageiro/metabolismo
RNA Ribossômico/metabolismo
RNA de Transferência/metabolismo
Ribossomos/metabolismo
Análise de Sequência de RNA
Transcrição Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chloroplast Proteins); 0 (Nucleic Acid Synthesis Inhibitors); 0 (Plant Proteins); 0 (RNA, Antisense); 0 (RNA, Chloroplast); 0 (RNA, Messenger); 0 (RNA, Ribosomal); 0 (RNA, Small Untranslated); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx668


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[PMID]:28498940
[Au] Autor:Li Q; Song A; Peng W; Jin Z; Müller WEG; Wang X
[Ad] Endereço:Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, PR China.
[Ti] Título:Contribution of aerobic anoxygenic phototrophic bacteria to total organic carbon pool in aquatic system of subtropical karst catchments, Southwest China: evidence from hydrochemical and microbiological study.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aerobic anoxygenic phototrophic bacteria may play a particular role in carbon cycling of aquatic systems. However, little is known about the interaction between aerobic anoxygenic phototrophic bacteria and hydrochemistry in groundwater-surface water exchange systems of subtropical karst catchments. We carried out a detailed study on the abundance of aerobic anoxygenic phototrophic bacteria and bacterioplankton, hydrochemistry and taxonomy of bacterioplankton in the Maocun watershed, Southwest China, an area with karst geological background. Our results revealed that bacteria are the important contributors to total organic carbon source/sequestration in the groundwater-surface water of this area. The aerobic anoxygenic phototrophic bacteria, including ß-Proteobacteria, also appear in the studied water system. In addition to that, the genus Polynucleobacter of the phototropic ß-Proteobacteria shows a close link with those sampling sites by presenting bacterial origin organic carbon on CCA biplot and is found to be positively correlated with total nitrogen, dissolved oxygen and pH (r = 0.860, 0.747 and 0.813, respectively) in the Maocun watershed. The results suggest that Polynucleobacter might be involved in the production of organic carbon and might act as the negative feedback on global warming.
[Mh] Termos MeSH primário: Betaproteobacteria/metabolismo
Carbono/metabolismo
Água Subterrânea/microbiologia
Processos Fototróficos/fisiologia
[Mh] Termos MeSH secundário: Organismos Aquáticos/metabolismo
Bactérias Aeróbias/classificação
Bactérias Aeróbias/genética
Bactérias Aeróbias/metabolismo
Betaproteobacteria/classificação
Betaproteobacteria/genética
Carbono/análise
China
Aquecimento Global
Nitrogênio/análise
Oxigênio/metabolismo
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 7440-44-0 (Carbon); N762921K75 (Nitrogen); S88TT14065 (Oxygen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix065


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[PMID]:28489281
[Au] Autor:Saghaï A; Gutiérrez-Preciado A; Deschamps P; Moreira D; Bertolino P; Ragon M; López-García P
[Ad] Endereço:Ecologie Systématique Evolution, CNRS, Université Paris-Sud, AgroParisTech, Université Paris-Saclay, Orsay, France.
[Ti] Título:Unveiling microbial interactions in stratified mat communities from a warm saline shallow pond.
[So] Source:Environ Microbiol;19(6):2405-2421, 2017 Jun.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Modern phototrophic microbial mats are complex communities often used as analogs of major Precambrian ecosystems. Characterizing biotic, notably metabolic, interactions among different microbial mat members is essential to gain insights into the ecology and biogeochemistry of these systems. We applied 16S/18S rRNA metabarcoding approaches to characterize the structure of archaea, bacteria and protist communities from microbial mats collected along strong physicochemical (oxygen, salinity, temperature, depth) gradients in a shallow pond at the salar de Llamara (Chile). All mats were highly diverse, including members of virtually all known high-rank eukaryotic and prokaryotic taxa but also many novel lineages. Bacterial candidate divisions accounted for almost 50% of sequences in deeper mats, while Archaea represented up to 40% of sequences in some mat layers. Molecular phylogenetic analyses revealed six novel deeply divergent archaeal groups, along abundant and diverse Pacearchaeota and Woesearchaeota. Multivariate statistical analyses showed that local environmental conditions strongly influenced community composition. Co-occurrence network structure was markedly different between surface mats located in the oxygenated zone and mats located in transition and anoxic water layers. We identified potential biotic interactions between various high- and low-rank taxa. Notably, a strong positive correlation was observed between Lokiarchaeota and the poorly known candidate bacterial division TA06.
[Mh] Termos MeSH primário: Archaea/classificação
Bactérias/classificação
Biofilmes/classificação
Interações Microbianas/fisiologia
Parasitos/classificação
Tanques/microbiologia
[Mh] Termos MeSH secundário: Animais
Archaea/genética
Bactérias/genética
Biodiversidade
Biofilmes/crescimento & desenvolvimento
Chile
Ecossistema
Parasitos/genética
Processos Fototróficos/fisiologia
Filogenia
RNA Ribossômico 16S/genética
RNA Ribossômico 18S/genética
Salinidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13754


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[PMID]:28346542
[Au] Autor:Somogyi B; Pálffy K; V-Balogh K; Botta-Dukát Z; Vörös L
[Ad] Endereço:Department of Hydrobotany, Balaton Limnological Institute, MTA Centre for Ecological Research, Tihany, Hungary.
[Ti] Título:Unusual behaviour of phototrophic picoplankton in turbid waters.
[So] Source:PLoS One;12(3):e0174316, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Autotrophic picoplankton (APP) abundance and contribution to phytoplankton biomass was studied in Hungarian shallow lakes to test the effect of inorganic turbidity determining the size distribution of the phytoplankton. The studied lakes displayed wide turbidity (TSS: 4-2250 mg l-1) and phytoplankton biomass (chlorophyll a: 1-460 µg l-1) range, as well as APP abundance (0 and 100 million cells ml-1) and contribution (0-100%) to total phytoplankton biomass. Inorganic turbidity had a significant effect on the abundance and contribution of APP, resulting in higher values compared to other freshwater lakes with the same phytoplankton biomass. Our analysis has provided empirical evidence for a switching point (50 mg l-1 inorganic turbidity), above which turbidity is the key factor causing APP predominance regardless of phytoplankton biomass in shallow turbid lakes. Our results have shown that turbid shallow lakes are unique waters, where the formerly and widely accepted model (decreasing APP contribution with increasing phytoplankton biomass) is not applicable. We hypothesize that this unusual behaviour of APP in turbid waters is a result of either diminished underwater light intensity or a reduced grazing pressure due to high inorganic turbidity.
[Mh] Termos MeSH primário: Biomassa
Cianobactérias/fisiologia
Lagos/química
Fitoplâncton/fisiologia
[Mh] Termos MeSH secundário: Clorofila/análise
Eutrofização
Hungria
Compostos Inorgânicos/análise
Modelos Biológicos
Nefelometria e Turbidimetria
Processos Fototróficos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inorganic Chemicals); 1406-65-1 (Chlorophyll); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174316


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[PMID]:27696020
[Au] Autor:Souza LD; Simioni C; Bouzon ZL; Schneider RC; Gressler P; Miotto MC; Rossi MJ; Rörig LR
[Ad] Endereço:Post Graduate Program in Biotechnology and Biosciences, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil. luanass1981@hotmail.com.
[Ti] Título:Morphological and ultrastructural characterization of the acidophilic and lipid-producer strain Chlamydomonas acidophila LAFIC-004 (Chlorophyta) under different culture conditions.
[So] Source:Protoplasma;254(3):1385-1398, 2017 May.
[Is] ISSN:1615-6102
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simultaneously assessing possible morphological and ultrastructural alterations in the cells. For heterotrophic and mixotrophic treatments, two organic carbon sources were tested: 1 % glucose and 1 % sodium acetate. Lipid content and fatty acid profiles were only determined in phototrophic condition. The higher growth rates were achieved in phototrophic conditions, varying from 0.18 to 0.82 day . Glucose did not result in significant growth increase in either mixotrophic or heterotrophic conditions, and acetate proved to be toxic to the strain in both conditions. Oil content under phototrophic condition was 15.9 % at exponential growth phase and increased to 54.63 % at stationary phase. Based on cell morphology (flow cytometry and light microscopy) and ultrastructure (transmission electron microscopy), similar characteristics were observed between phototrophic and mixotrophic conditions with glucose evidencing many lipid bodies, starch granules, and intense fluorescence. Under the tested conditions, mixotrophic and heterotrophic modes did not result in increased neutral lipid fluorescence. It can be concluded that the strain is a promising lipid producer when grown until stationary phase in acidic medium and under a phototrophic regime, presenting a fatty acid profile suitable for biodiesel production. The ability to grow this strain in acidic mining residues suggests a potential for bioremediation with production of useful biomass.
[Mh] Termos MeSH primário: Chlamydomonas/metabolismo
Chlamydomonas/ultraestrutura
Ácidos Graxos/biossíntese
Glucose/metabolismo
Processos Heterotróficos/fisiologia
Processos Fototróficos/fisiologia
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Biocombustíveis
Chlamydomonas/crescimento & desenvolvimento
Minas de Carvão
Gotículas Lipídicas/metabolismo
Microalgas/classificação
Microalgas/metabolismo
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Fatty Acids); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE
[do] DOI:10.1007/s00709-016-1030-7


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[PMID]:27585879
[Au] Autor:Fang L; Ge H; Huang X; Liu Y; Lu M; Wang J; Chen W; Xu W; Wang Y
[Ad] Endereço:State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Beijing 100101, China.
[Ti] Título:Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light-Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803.
[So] Source:Mol Plant;10(1):73-85, 2017 Jan 09.
[Is] ISSN:1752-9867
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The photosynthetic model organism Synechocystis sp. PCC 6803 can grow in different trophic modes, depending on the availability of light and exogenous organic carbon source. However, how the protein profile changes to facilitate the cells differentially propagate in different modes has not been comprehensively investigated. Using isobaric labeling-based quantitative proteomics, we simultaneously identified and quantified 45% Synechocystis proteome across four different trophic modes, i.e., autotrophic, heterotrophic, photoheterotrophic, and mixotrophic modes. Among the 155 proteins that are differentially expressed across four trophic modes, proteins involved in nitrogen assimilation and light-independent chlorophyll synthesis are dramatically upregulated in the mixotrophic mode, concomitant with a dramatic increase of P phosphorylation that senses carbon and nitrogen assimilation status. Moreover, functional study using a mutant defective in light-independent chlorophyll synthesis revealed that this pathway is important for chlorophyll accumulation under a cycled light/dark illumination regime, a condition mimicking day/night cycles in certain natural habitats. Collectively, these results provide the most comprehensive information on trophic mode-dependent protein expression in cyanobacterium, and reveal the functional significance of light-independent chlorophyll synthesis in trophic growth.
[Mh] Termos MeSH primário: Clorofila/biossíntese
Synechocystis/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Clorofila/efeitos da radiação
Escuridão
Luz
Fotossíntese
Processos Fototróficos
Proteoma
Synechocystis/crescimento & desenvolvimento
Synechocystis/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Proteome); 1406-65-1 (Chlorophyll)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160903
[St] Status:MEDLINE


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[PMID]:27530806
[Au] Autor:Koller AP; Löwe H; Schmid V; Mundt S; Weuster-Botz D
[Ad] Endereço:Institute of Biochemical Engineering, Technical University of Munich, Boltzmannstr. 15, 85748 Garching, Germany.
[Ti] Título:Model-supported phototrophic growth studies with Scenedesmus obtusiusculus in a flat-plate photobioreactor.
[So] Source:Biotechnol Bioeng;114(2):308-320, 2017 Feb.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Light-dependent growth of microalgae can vary remarkably depending on the cultivation system and microalgal strain. Cell size and the pigmentation of each strain, as well as reactor geometry have a great impact on absorption and scattering behavior within a photobioreactor. In this study, the light-dependent, cell-specific growth kinetics of a novel green algae isolate, Scenedesmus obtusiusculus, was studied in a LED-illuminated flat-plate photobioreactor on a lab-scale (1.8 L, 0.09 m ). First, pH-controlled batch processes were performed with S. obtusiusculus at different constant incident photon flux densities. The best performance was achieved by illuminating S. obtusiusculus with 1400 µmol photons m s at the surface of the flat-plate photobioreactor, resulting in the highest biomass concentration (4.95 ± 0.16 g L within 3.5 d) and the highest specific growth rate (0.22 h ). The experimental data were used to identify the kinetic parameters of different growth models considering light inhibition for S. obtusiusculus. Light attenuation within the flat-plate photobioreactor was considered by varying light transfer models. Based on the identified kinetic growth model of S. obtusiusculus, an optimum growth rate of 0.22 h was estimated at a mean integral photon flux density of 1072 µmol photons m s with the Beer-Lambert law and 1590 µmol photons m s with Schuster's light transfer model in the flat-plate photobioreactor. LED illumination was, thus, increased to keep the identified optimum mean integral photon flux density constant in the batch process assuming Schuster's light transfer model. Compared to the same constant incident photon flux density (1590 µmol photons m s ), biomass concentration was up to 24% higher using the lighting profile until a dry cell mass concentration of 14.4 ± 1.4 g L was reached. Afterward, the biomass concentration remained constant, whereas cell growth continued in the batch process with constant incident photon flux density. Finally, biomass concentration was 15.5 ± 1.5 g L and, thus, 7% higher compared to the corresponding batch process with lighting profile. Biotechnol. Bioeng. 2017;114: 308-320. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Fotobiorreatores
Processos Fototróficos/fisiologia
Scenedesmus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Biomassa
Cinética
Microalgas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160818
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26072


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[PMID]:27889482
[Au] Autor:Shi H; Shen X; Liu R; Xue C; Wei N; Deng XW; Zhong S
[Ad] Endereço:State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China.
[Ti] Título:The Red Light Receptor Phytochrome B Directly Enhances Substrate-E3 Ligase Interactions to Attenuate Ethylene Responses.
[So] Source:Dev Cell;39(5):597-610, 2016 Dec 05.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants germinating under subterranean darkness assume skotomorphogenesis, a developmental program strengthened by ethylene in response to mechanical pressure of soil. Upon reaching the surface, light triggers a dramatic developmental transition termed de-etiolation that requires immediate termination of ethylene responses. Here, we report that light activation of photoreceptor phyB results in rapid degradation of EIN3, the master transcription factor in the ethylene signaling pathway. As a result, light rapidly and efficiently represses ethylene actions. Specifically, phyB directly interacts with EIN3 in a light-dependent manner and also physically associates with F box protein EBFs. The light-activated association of phyB, EIN3, and EBF1/EBF2 proteins stimulates robust EIN3 degradation by SCF E3 ligases. We reveal that phyB manipulates substrate-E3 ligase interactions in a light-dependent manner, thus directly controlling the stability of EIN3. Our findings illustrate a mechanistic model of how plants transduce light information to immediately turn off ethylene signaling for de-etiolation initiation.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Etilenos/metabolismo
Proteínas F-Box/metabolismo
Proteínas Nucleares/metabolismo
Fitocromo B/metabolismo
Fatores de Transcrição/metabolismo
Ubiquitina-Proteína Ligases/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/efeitos da radiação
Proteínas F-Box/genética
Luz
Modelos Biológicos
Mutação
Proteínas Nucleares/genética
Processos Fototróficos
Fitocromo B/efeitos da radiação
Plantas Geneticamente Modificadas
Complexo de Endopeptidases do Proteassoma/metabolismo
Ligação Proteica/efeitos da radiação
Proteólise/efeitos da radiação
Transdução de Sinais
Fatores de Transcrição/genética
Ubiquitina-Proteína Ligases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (EBF1 protein, Arabidopsis); 0 (EBF2 protein, Arabidopsis); 0 (EIN3 protein, Arabidopsis); 0 (Ethylenes); 0 (F-Box Proteins); 0 (Nuclear Proteins); 0 (PHYB protein, Arabidopsis); 0 (Transcription Factors); 136250-22-1 (Phytochrome B); 91GW059KN7 (ethylene); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 3.4.25.1 (Proteasome Endopeptidase Complex); EC 3.4.99.- (ATP dependent 26S protease)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE
[do] DOI:10.1016/j.devcel.2016.10.020



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