Base de dados : MEDLINE
Pesquisa : G02.111.760.250 [Categoria DeCS]
Referências encontradas : 3125 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 313 ir para página                         

  1 / 3125 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29229384
[Au] Autor:Yang J; Zhang M; Wang X
[Ad] Endereço:School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui 230601, China.
[Ti] Título:Crystal structure of the chloroplast RNA editing factor MORF2.
[So] Source:Biochem Biophys Res Commun;495(2):2038-2043, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA editing is a post-transcription process that alters the genetic information on RNA molecules. In plastids and mitochondria of flowering plants, the multiple organellar RNA editing factors (MORFs) interact with the PLS-type pentatricopeptide repeat (PPR) proteins and participate in RNA editing of cytidine-to-uridine conversion. The PPR proteins recognize cytidine targets around the editing sites, and the MORF proteins modulate the RNA-binding activity of the PPR proteins. Here, we report the structure of the Arabidopsis thaliana chloroplast MORF2 at 2.4 Å resolution. The structure, adopting typical MORF-box fold as observed in mitochondrial MORF1 and chloroplast MORF9, reveals an MORF1-like dimerization mode. The difference between the two dimerization modes can be attributed to F157 (corresponding F162 in MORF1 and W160 in MORF9), which causes a 60° shift upon dimerization. This observation, together with the PPR-MORF2 model, suggests a dimer-to-monomer transition during RNA editosome formation.
[Mh] Termos MeSH primário: Arabidopsis/química
Arabidopsis/ultraestrutura
Cloroplastos/ultraestrutura
RNA de Cloroplastos/ultraestrutura
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis
Cloroplastos/química
Proteínas Mitocondriais
Conformação Proteica
Edição de RNA
RNA de Cloroplastos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (MORF2 protein, Arabidopsis); 0 (Mitochondrial Proteins); 0 (RNA, Chloroplast)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  2 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27776542
[Au] Autor:Danan-Gotthold M; Guyon C; Giraud M; Levanon EY; Abramson J
[Ad] Endereço:The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel.
[Ti] Título:Extensive RNA editing and splicing increase immune self-representation diversity in medullary thymic epithelial cells.
[So] Source:Genome Biol;17(1):219, 2016 10 24.
[Is] ISSN:1474-760X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In order to become functionally competent but harmless mediators of the immune system, T cells undergo a strict educational program in the thymus, where they learn to discriminate between self and non-self. This educational program is, to a large extent, mediated by medullary thymic epithelial cells that have a unique capacity to express, and subsequently present, a large fraction of body antigens. While the scope of promiscuously expressed genes by medullary thymic epithelial cells is well-established, relatively little is known about the expression of variants that are generated by co-transcriptional and post-transcriptional processes. RESULTS: Our study reveals that in comparison to other cell types, medullary thymic epithelial cells display significantly higher levels of alternative splicing, as well as A-to-I and C-to-U RNA editing, which thereby further expand the diversity of their self-antigen repertoire. Interestingly, Aire, the key mediator of promiscuous gene expression in these cells, plays a limited role in the regulation of these transcriptional processes. CONCLUSIONS: Our results highlight RNA processing as another layer by which the immune system assures a comprehensive self-representation in the thymus which is required for the establishment of self-tolerance and prevention of autoimmunity.
[Mh] Termos MeSH primário: Células Epiteliais/imunologia
Edição de RNA/genética
Timo/imunologia
[Mh] Termos MeSH secundário: Processamento Alternativo/genética
Processamento Alternativo/imunologia
Animais
Autoantígenos/genética
Autoantígenos/imunologia
Diferenciação Celular/imunologia
Regulação da Expressão Gênica/imunologia
Seres Humanos
Camundongos
Edição de RNA/imunologia
Tolerância a Antígenos Próprios/imunologia
Linfócitos T/imunologia
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Autoantigens); 0 (Transcription Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  3 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28453786
[Au] Autor:Porath HT; Schaffer AA; Kaniewska P; Alon S; Eisenberg E; Rosenthal J; Levanon EY; Levy O
[Ad] Endereço:The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
[Ti] Título:A-to-I RNA Editing in the Earliest-Diverging Eumetazoan Phyla.
[So] Source:Mol Biol Evol;34(8):1890-1901, 2017 Aug 01.
[Is] ISSN:1537-1719
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The highly conserved ADAR enzymes, found in all multicellular metazoans, catalyze the editing of mRNA transcripts by the deamination of adenosines to inosines. This type of editing has two general outcomes: site specific editing, which frequently leads to recoding, and clustered editing, which is usually found in transcribed genomic repeats. Here, for the first time, we looked for both editing of isolated sites and clustered, non-specific sites in a basal metazoan, the coral Acropora millepora during spawning event, in order to reveal its editing pattern. We found that the coral editome resembles the mammalian one: it contains more than 500,000 sites, virtually all of which are clustered in non-coding regions that are enriched for predicted dsRNA structures. RNA editing levels were increased during spawning and increased further still in newly released gametes. This may suggest that editing plays a role in introducing variability in coral gametes.
[Mh] Termos MeSH primário: Adenosina Desaminase/genética
Antozoários/genética
Edição de RNA/genética
[Mh] Termos MeSH secundário: Adenosina Desaminase/metabolismo
Animais
Antozoários/metabolismo
Sequência de Bases
Evolução Molecular
Genoma
Genômica
Seres Humanos
Mamíferos/genética
Filogenia
RNA
RNA Mensageiro/genética
Proteínas de Ligação a RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (RNA, immune); 0 (RNA-Binding Proteins); 63231-63-0 (RNA); EC 3.5.4.4 (Adenosine Deaminase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/molbev/msx125


  4 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29223164
[Au] Autor:Goryunov DV; Logacheva MD; Ignatov MS; Milyutina IA; Fedorova AV; Troitsky AV
[Ad] Endereço:Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. bobr@belozersky.msu.ru.
[Ti] Título:The Mitochondrial Genome of the Moss Brachythecium rivulare (Hypnales, Brachytheciaceae).
[So] Source:Biochemistry (Mosc);82(11):1373-1379, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mitochondrial genome of the pleurocarpous moss Brachythecium rivulare has been sequenced and annotated. The genome consists of 104,460 base pairs and has approximately the same gene set and organization as other bryophyte mitogenomes. Whole mitochondrial genome comparison between B. rivulare and Physcomitrella patens, Tetraphis pellucida, Anomodon rugelii, and Anomodon attenuatus was performed. The primary cause of bryophyte mitochondrial gene length variation was found to be numerous indels in the introns. Bryophyte mitochondrial gene conservation level was estimated, and it was in a good congruence with the overall phylogeny of bryophytes with the percentage of mitogenome similarity being proportional to the age estimated by phylochronologic analysis. Annotation discrepancies in the analyzed mitogenome sequences were identified. The simple sequence repeat (SSR) content was evaluated, and candidate sites of RNA editing were predicted in the B. rivulare mitochondrial genome.
[Mh] Termos MeSH primário: Briófitas/genética
Genoma Mitocondrial/genética
[Mh] Termos MeSH secundário: Íntrons/genética
Repetições de Microssatélites
Anotação de Sequência Molecular
Edição de RNA
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110153


  5 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29182635
[Au] Autor:Yablonovitch AL; Deng P; Jacobson D; Li JB
[Ad] Endereço:Stanford University, Department of Genetics, Stanford, California, United States of America.
[Ti] Título:The evolution and adaptation of A-to-I RNA editing.
[So] Source:PLoS Genet;13(11):e1007064, 2017 Nov.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Adenosine-to-inosine (A-to-I) RNA editing is an important post-transcriptional modification that affects the information encoded from DNA to RNA to protein. RNA editing can generate a multitude of transcript isoforms and can potentially be used to optimize protein function in response to varying conditions. In light of this and the fact that millions of editing sites have been identified in many different species, it is interesting to examine the extent to which these sites have evolved to be functionally important. In this review, we discuss results pertaining to the evolution of RNA editing, specifically in humans, cephalopods, and Drosophila. We focus on how comparative genomics approaches have aided in the identification of sites that are likely to be advantageous. The use of RNA editing as a mechanism to adapt to varying environmental conditions will also be reviewed.
[Mh] Termos MeSH primário: Edição de RNA/genética
Edição de RNA/fisiologia
RNA/genética
[Mh] Termos MeSH secundário: Aclimatação/genética
Adaptação Fisiológica/genética
Adenosina/genética
Adenosina/metabolismo
Animais
Sequência de Bases/genética
Evolução Molecular
Genômica/métodos
Seres Humanos
Inosina/genética
Inosina/metabolismo
RNA/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
5A614L51CT (Inosine); 63231-63-0 (RNA); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007064


  6 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28453770
[Au] Autor:Yang J; Harding T; Kamikawa R; Simpson AGB; Roger AJ
[Ad] Endereço:Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
[Ti] Título:Mitochondrial Genome Evolution and a Novel RNA Editing System in Deep-Branching Heteroloboseids.
[So] Source:Genome Biol Evol;9(5):1161-1174, 2017 05 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Discoba (Excavata) is an evolutionarily important group of eukaryotes that includes Jakobida, with the most bacterial-like mitochondrial genomes known, and Euglenozoa, many of which have extensively fragmented mitochondrial genomes. However, little is known about the mitochondrial genomes of Heterolobosea, the third main group of Discoba. Here, we studied two heteroloboseids-an undescribed amoeba "BB2" and Pharyngomonas kirbyi. Phylogenomic analysis revealed that they form a clade that is a sister group to all other Heterolobosea. We characterized the mitochondrial genomes of BB2 and P. kirbyi, which encoded 44 and 48 putative protein-coding genes respectively. Their gene contents were similar to that of Naegleria. In BB2, mitochondrially encoded RNAs were heavily edited, with ∼500 mononucleotide insertion events, mostly guanosines. These insertions always have the same identity as an adjacent nucleotide. Editing occurs in all ribosomal RNAs and protein-coding transcripts except one, and half of the transfer RNAs. Analysis of Illumina deep-sequencing data suggested that this RNA editing is very accurate and efficient, and most likely co-transcriptional. The dissimilarity of this editing process to other RNA editing phenomena in discobids, as well as its apparent absence in P. kirbyi, suggest that this remarkably extensive system of insertional editing evolved independently in the BB2 lineage, after its divergence from the P. kirbyi lineage.
[Mh] Termos MeSH primário: Eucariotos/genética
Genoma Mitocondrial
Edição de RNA
[Mh] Termos MeSH secundário: Sequência de Bases
Evolução Biológica
Eucariotos/classificação
Filogenia
RNA de Transferência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx086


  7 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29031774
[Au] Autor:Damsteegt EL; Davie A; Lokman PM
[Ad] Endereço:Department of Zoology, University of Otago, 340 Great King Street, PO Box 56, Dunedin 9054, New Zealand. Electronic address: erin.damsteegt@otago.ac.nz.
[Ti] Título:The evolution of apolipoprotein B and its mRNA editing complex. Does the lack of editing contribute to hypertriglyceridemia?
[So] Source:Gene;641:46-54, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The evolution of apolipoprotein B (Apob) has been intensely researched due to its importance during lipid transport. Mammalian full-length apob100 can be post-transcriptionally edited by the enzyme apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like complex-one (Apobec1) resulting in a truncated Apob, known as Apob48. Whilst both full-length and truncated forms of Apob are important for normal lipid homeostasis in mammals, there is no evidence for the presence of apob mRNA editing prior to the divergence of the mammals, yet, non-mammalian vertebrates appear to function normally with only Apob100. To date, the majority of the research carried out in non-mammalian vertebrates has focused on chickens with only a very limited number examining apob mRNA editing in fish. This study focused on the molecular evolution of Apobec1 and Apob in order to ascertain if apob mRNA editing occurs in eels, a basal teleost which represents an evolutionarily important animal group. No evidence for the presence of Apobec1 or the ability for eel apob to be edited was found. However, an important link between mutant mice and the evident hypertriglyceridemia in the plasma of non-mammalian vertebrates was made. This study has provided imperative evidence to help bridge the evolutionary gap between fish and mammals and provides further support for the lack of apob mRNA editing in non-mammalian vertebrates.
[Mh] Termos MeSH primário: Desaminases APOBEC/genética
Apolipoproteínas B/genética
Hipertrigliceridemia/genética
Edição de RNA/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anguilla/genética
Animais
Mapeamento Cromossômico
Seres Humanos
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apolipoproteins B); EC 3.5.4.5 (APOBEC Deaminases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


  8 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29022489
[Au] Autor:Song IH; Kim YA; Heo SH; Park IA; Lee M; Bang WS; Park HS; Gong G; Lee HJ
[Ad] Endereço:1 Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
[Ti] Título:ADAR1 expression is associated with tumour-infiltrating lymphocytes in triple-negative breast cancer.
[So] Source:Tumour Biol;39(10):1010428317734816, 2017 Oct.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tumours with a high mutation burden exhibit considerable neoantigens and tumour-infiltrating lymphocytes. RNA editing by ADAR1 is a source of changes in epitope. However, ADAR1 expression in cancer cells and tumour-infiltrating lymphocyte levels in triple-negative breast cancer have not been well evaluated. We immunohistochemically examined ADAR1 expression in 681 triple-negative breast cancer patients and analysed their clinicopathological characteristics. We also analysed basal-like tumours using The Cancer Genome Atlas data. Among the 681 triple-negative breast cancer patients, 45.8% demonstrated high ADAR1 expression. Tumours with high ADAR1 expression exhibited high tumour-infiltrating lymphocyte levels, considerable CD8 + T lymphocyte infiltration, high histological grade and high expression of interferon-related proteins, including HLA-ABC, MxA and PKR. Among patients with lymph node metastasis, those with high tumour-infiltrating lymphocyte levels and low ADAR1 expression demonstrated the best disease-free survival. The Cancer Genome Atlas data analysis of basal-like tumours revealed significant positive correlation between ADAR1 and CD8B expression and positive association of high ADAR1 expression with immune responses and apoptosis pathways. We detected high ADAR1 expression in half of the triple-negative breast cancer patients. In addition to DNA mutations, RNA editing can be related to neoantigens; hence, we need to explore non-synonymous mutations exclusively found using RNA sequencing data to identify clinically relevant neoantigens.
[Mh] Termos MeSH primário: Adenosina Desaminase/biossíntese
Biomarcadores Tumorais/biossíntese
Proteínas de Ligação a RNA/biossíntese
Neoplasias de Mama Triplo Negativas/genética
[Mh] Termos MeSH secundário: Adenosina Desaminase/genética
Adulto
Idoso
Biomarcadores Tumorais/genética
Linfócitos T CD8-Positivos/patologia
Intervalo Livre de Doença
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Metástase Linfática
Linfócitos do Interstício Tumoral/patologia
Meia-Idade
Edição de RNA/genética
Proteínas de Ligação a RNA/genética
Análise Serial de Tecidos
Neoplasias de Mama Triplo Negativas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (RNA-Binding Proteins); EC 3.5.4.37 (ADAR1 protein, human); EC 3.5.4.4 (Adenosine Deaminase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317734816


  9 / 3125 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28991908
[Au] Autor:Kirby LE; Koslowsky D
[Ad] Endereço:Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
[Ti] Título:Mitochondrial dual-coding genes in Trypanosoma brucei.
[So] Source:PLoS Negl Trop Dis;11(10):e0005989, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trypanosoma brucei is transmitted between mammalian hosts by the tsetse fly. In the mammal, they are exclusively extracellular, continuously replicating within the bloodstream. During this stage, the mitochondrion lacks a functional electron transport chain (ETC). Successful transition to the fly, requires activation of the ETC and ATP synthesis via oxidative phosphorylation. This life cycle leads to a major problem: in the bloodstream, the mitochondrial genes are not under selection and are subject to genetic drift that endangers their integrity. Exacerbating this, T. brucei undergoes repeated population bottlenecks as they evade the host immune system that would create additional forces of genetic drift. These parasites possess several unique genetic features, including RNA editing of mitochondrial transcripts. RNA editing creates open reading frames by the guided insertion and deletion of U-residues within the mRNA. A major question in the field has been why this metabolically expensive system of RNA editing would evolve and persist. Here, we show that many of the edited mRNAs can alter the choice of start codon and the open reading frame by alternative editing of the 5' end. Analyses of mutational bias indicate that six of the mitochondrial genes may be dual-coding and that RNA editing allows access to both reading frames. We hypothesize that dual-coding genes can protect genetic information by essentially hiding a non-selected gene within one that remains under selection. Thus, the complex RNA editing system found in the mitochondria of trypanosomes provides a unique molecular strategy to combat genetic drift in non-selective conditions.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica/fisiologia
Mitocôndrias/genética
Trypanosoma brucei brucei/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Sequência Conservada
Mutação
Proteínas de Protozoários/metabolismo
Edição de RNA
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Trypanosoma brucei brucei/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protozoan Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171105
[Lr] Data última revisão:
171105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005989


  10 / 3125 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28985428
[Au] Autor:Qi L; Song Y; Chan THM; Yang H; Lin CH; Tay DJT; Hong H; Tang SJ; Tan KT; Huang XX; Lin JS; Ng VHE; Maury JJP; Tenen DG; Chen L
[Ad] Endereço:Cancer Science Institute of Singapore, National University of Singapore, Singapore.
[Ti] Título:An RNA editing/dsRNA binding-independent gene regulatory mechanism of ADARs and its clinical implication in cancer.
[So] Source:Nucleic Acids Res;45(18):10436-10451, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by Adenosine DeAminases acting on double-stranded RNA(dsRNA) (ADAR), occurs predominantly in the 3' untranslated regions (3'UTRs) of spliced mRNA. Here we uncover an unanticipated link between ADARs (ADAR1 and ADAR2) and the expression of target genes undergoing extensive 3'UTR editing. Using METTL7A (Methyltransferase Like 7A), a novel tumor suppressor gene with multiple editing sites at its 3'UTR, we demonstrate that its expression could be repressed by ADARs beyond their RNA editing and double-stranded RNA (dsRNA) binding functions. ADARs interact with Dicer to augment the processing of pre-miR-27a to mature miR-27a. Consequently, mature miR-27a targets the METTL7A 3'UTR to repress its expression level. In sum, our study unveils that the extensive 3'UTR editing of METTL7A is merely a footprint of ADAR binding, and there are a subset of target genes that are equivalently regulated by ADAR1 and ADAR2 through their non-canonical RNA editing and dsRNA binding-independent functions, albeit maybe less common. The functional significance of ADARs is much more diverse than previously appreciated and this gene regulatory function of ADARs is most likely to be of high biological importance beyond the best-studied editing function. This non-editing side of ADARs opens another door to target cancer.
[Mh] Termos MeSH primário: Adenosina Desaminase/metabolismo
Redes Reguladoras de Genes/fisiologia
Neoplasias/genética
Edição de RNA
RNA de Cadeia Dupla/metabolismo
Proteínas de Ligação a RNA/metabolismo
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas/genética
Adenosina/metabolismo
Animais
Regulação Neoplásica da Expressão Gênica
Células HEK293
Seres Humanos
Inosina/metabolismo
Neoplasias/metabolismo
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (RNA, Double-Stranded); 0 (RNA-Binding Proteins); 5A614L51CT (Inosine); EC 3.5.4.4 (Adenosine Deaminase); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx667



página 1 de 313 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde