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[PMID]:29346433
[Au] Autor:Chen J; OuYang H; An X; Liu S
[Ad] Endereço:Department of E.N.T., West China Hospital, Sichuan University, Chengdu, China.
[Ti] Título:Vault RNAs partially induces drug resistance of human tumor cells MCF-7 by binding to the RNA/DNA-binding protein PSF and inducing oncogene GAGE6.
[So] Source:PLoS One;13(1):e0191325, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Vault is the largest nonicosahedral cytosolic nucleoprotein particle, which is widely involved in induction of chemoresistance and lead to failure in long-term chemotherapy. Vault contains three different major vault proteins (MVPs) and four vault RNAs paralogues (vtRNAs, vtRNA1-1, vtRNA1-2, vtRNA1-3 and vtRNA2-1). Disruption of the MVPs do not induce hypersensitivity while expression of vtRNAs contributes to cells' drug resistance, indicates that vtRNAs, but not MVPs play an important role in causing drug resistance. Polypyrimidine tract binding protein associated splicing factor (PSF) contributes to cell sensitivity to chemotherapy by its transcriptional activity, promotes us to figure out its potential association with vtRNAs. METHODS: We investigate the interaction between PSF and vtRNAs by electrophoretic mobility shift assays (EMSA) and RNA-immunoprecipitation (IP), and showed the binding between PSF and vtRNAs. Chromatin Immunoprecipitation (ChIP) was performed to detect the effects of vtRNAs on the interaction of PSF with GAGE6 promoter. The role of vtRNAs on chemoresistance in MCF-7 was detected by CCK-8 and EdU staining. The independent role of vtRNAs with MVP is detected by MVP or vtRNAs knockdown. RESULTS: The complex with vtRNA1-1 releases PSF, allowing transcription of GAGE6 to proceed. Then we showed that induction of GAGE6 caused drug resistance by promoting cell proliferation and colony formation in soft agar. Ectopic expression of shRNA targets to vtRNA1-1 further confirmed the role of vtRNA1-1 in regulating PSF transcriptional activity independent with the expression of MVP. By vtRNA1-1 or MVP knockdown, it is revealed that vtRNA1-1 caused chemoresistance independent of MVP. Furthermore, knockdown of GAGE6 does not cause drug resistance, indicates the GAGE6 is directly involved in cell proliferation, but not the drug resistance. CONCLUSION: These results suggest that vtRNAs regulates cell proliferation, drug resistance, and possibly other physiological processes of humans, by complex formation with PSF.
[Mh] Termos MeSH primário: Resistência a Medicamentos Antineoplásicos/genética
Oncogenes/genética
Fator de Processamento Associado a PTB/metabolismo
RNA/genética
RNA/metabolismo
Partículas de Ribonucleoproteínas em Forma de Abóbada/genética
[Mh] Termos MeSH secundário: Proliferação Celular/efeitos dos fármacos
Doxorrubicina/farmacologia
Seres Humanos
Células MCF-7
Fator de Processamento Associado a PTB/química
Regiões Promotoras Genéticas/genética
Ligação Proteica
Domínios Proteicos
Homologia de Sequência do Ácido Nucleico
Transcrição Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (PTB-Associated Splicing Factor); 0 (Vault Ribonucleoprotein Particles); 63231-63-0 (RNA); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191325


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[PMID]:29385200
[Au] Autor:Rout ED; Burnett RC; Labadie JD; Yoshimoto JA; Avery AC
[Ad] Endereço:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.
[Ti] Título:Preferential use of unmutated immunoglobulin heavy variable region genes in Boxer dogs with chronic lymphocytic leukemia.
[So] Source:PLoS One;13(1):e0191205, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human chronic lymphocytic leukemia (CLL) is a clinically heterogeneous disease, and immunoglobulin heavy variable region (IGHV) gene mutational status is an important prognostic marker. IGHV mutational status has not been previously examined in canine CLL. We sequenced the IGHV-D-J rearrangements from 55 canine patients with CLL, including 36 non-Boxer and 19 Boxer dogs. The majority of non-Boxers (75%) had mutated IGHV genes, whereas the majority of Boxers (79%) had unmutated IGHV genes. IGHV3-41 and IGHV3-67 gene usage was significantly higher in Boxers with CLL compared to non-Boxers. Additionally, 11 Boxers with large B-cell lymphoma and the normal IGHV repertoire of six control dogs (three Boxers and three non-Boxers) were sequenced. IGHV3-41 was preferentially used in Boxers with other forms of lymphoma and without lymphoproliferative disease. However, preferential use of unmutated IGHV genes was unique to Boxers with CLL, suggesting Boxers may be a valuable model to investigate unmutated CLL.
[Mh] Termos MeSH primário: Doenças do Cão/genética
Doenças do Cão/imunologia
Genes de Cadeia Pesada de Imunoglobulina
Região Variável de Imunoglobulina/genética
Leucemia Linfocítica Crônica de Células B/veterinária
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Estudos de Casos e Controles
Análise Mutacional de DNA
DNA de Neoplasias/genética
Cães
Feminino
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Leucemia Linfocítica Crônica de Células B/genética
Leucemia Linfocítica Crônica de Células B/imunologia
Masculino
Mutação
Homologia de Sequência do Ácido Nucleico
Especificidade da Espécie
Éxons VDJ
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Neoplasm); 0 (Immunoglobulin Variable Region)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191205


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[PMID]:29224130
[Au] Autor:Wang M; Wang Y; Baloch AR; Pan Y; Xu F; Tian L; Zeng Q
[Ad] Endereço:College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
[Ti] Título:Molecular epidemiology and characterization of bovine leukemia virus in domestic yaks (Bos grunniens) on the Qinghai-Tibet Plateau, China.
[So] Source:Arch Virol;163(3):659-670, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV infections in domestic yaks and determine the genetic variability of BLV circulating in a region of the Qinghai Tibet Plateau, China. Blood samples were collected from 798 yaks, which were from different farms from Gansu, Qinghai and Sichuan provinces surrounding the Qinghai-Tibet Plateau. Nested PCR targeting BLV long terminal repeats was used to detect the BLV provirus. The highest prevalence of BLV infection was in Gansu province, where it was 18.93% (39/206) in white yaks from Tianzhu City and 19.14% (31/162) in black yaks from Gannan City. In Qinghai and Sichuan provinces, the prevalence of BLV in black yaks was 14.83% (35/236) and 14.94% (29/194), respectively. The prevalence of BLV was not significantly different in yaks up to one year old than in older animals. Phylogenetic analysis was performed using 16 different env-gp51 (497-bp) gene sequences from the three provinces and 71 known BLV strains, which revealed that in both Gansu and Qinghai provinces, genotypes 6 and 10 of the BLV strains were at high levels, whereas only genotype 10 was prevalent in Sichuan Province. Phylogenetic analysis and sequence comparisons revealed 95.7-99.8% sequence identity among the full-length env genes of 16 strains, nearly full-length genome sequences of six BLV strains, and those of the known genotypes 6 and 10 of BLV. This study provides comprehensive information is regarding the widespread infection of domestic yaks with BLV on the Qinghai-Tibet Plateau of China, and shows that at least two BLV genotypes (genotypes 6 and 10) are circulating in this population.
[Mh] Termos MeSH primário: Leucose Enzoótica Bovina/epidemiologia
Genes env
Genótipo
Vírus da Leucemia Bovina/classificação
Vírus da Leucemia Bovina/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Bovinos
Leucose Enzoótica Bovina/transmissão
Leucose Enzoótica Bovina/virologia
Expressão Gênica
Vírus da Leucemia Bovina/isolamento & purificação
Epidemiologia Molecular
Prevalência
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Sequências Repetidas Terminais
Tibet/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3658-9


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[PMID]:29224129
[Au] Autor:Gibbs AJ; Gibbs MJ
[Ad] Endereço:Emeritus Faculty, Australian National University, Canberra, ACT, 2601, Australia. adrian_j_gibbs@hotmail.com.
[Ti] Título:Rymovirus: a cautionary tale.
[So] Source:Arch Virol;163(3):815-817, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A recent proposal that the genus Rymovirus be assimilated into the genus Potyvirus is examined, discussed, and rejected. It illustrates the danger of using 'sequence identity' as a proxy for phylogenetic relatedness to distinguish closely related but distinct groups of viruses.
[Mh] Termos MeSH primário: Genoma Viral
Filogenia
Potyviridae/classificação
Potyvirus/classificação
RNA Viral/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Evolução Biológica
Biologia Computacional/métodos
Potyviridae/genética
Potyvirus/genética
Análise de Sequência de RNA
Homologia de Sequência do Ácido Nucleico
Terminologia como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3676-7


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[PMID]:29224128
[Au] Autor:Anwar S; Tahir M
[Ad] Endereço:Plant Biotechnology Department, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, Pakistan.
[Ti] Título:Identification of a new begomovirus infecting Duranta repens in Pakistan.
[So] Source:Arch Virol;163(3):809-813, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Ornamental perennial plants may serve as reservoirs for viruses that infect field crops. Duranta repens is an ornamental shrub that frequently exhibits leaf curl symptoms typical of begomoviral infection. Two begomoviruses or DNA-A components (isolates 57SA and 58SA) along with a DNA-B component (31SA) were identified in a symptomatic D. repens plant. Isolates 57SA and 58SA showed the highest nucleotide sequence identity of 90% and 92.2% to catharanthus yellow mosaic virus (CaYMV) and chilli leaf curl India virus (ChiLCINV), respectively, whereas 31SA shared the highest nucleotide sequence identity of 95.2% with the DNA-B component of tomato leaf curl New Delhi Virus (ToLCNDV). Isolate 57SA (showing < 91% highest nucleotide sequence identity) may thus be considered a member of a distinct species, and we propose the name "duranta leaf curl virus" (DLCV) for this virus. In addition, isolate 58SA (showing highest nucleotide sequence identity < 94%) is a newly identified strain of ChiLCINV for which the name "Duranta" strain is proposed. Further analysis showed that 57SA, 58SA and 31SA are recombinant, again highlighting the importance of recombination in begomovirus evolution. This is the first identification of DLCV. Also, a mixed infection with two viruses (or DNA-A components) and one DNA-B is reported.
[Mh] Termos MeSH primário: Begomovirus/genética
DNA Viral/genética
Genoma Viral
Filogenia
Verbenaceae/virologia
[Mh] Termos MeSH secundário: Sequência de Bases
Begomovirus/classificação
Begomovirus/isolamento & purificação
Sequenciamento de Nucleotídeos em Larga Escala
Paquistão
Doenças das Plantas/virologia
Recombinação Genética
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3672-y


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[PMID]:29209812
[Au] Autor:Bejerman N; Trucco V; de Breuil S; Pardina PR; Lenardon S; Giolitti F
[Ad] Endereço:IPAVE-CIAP-INTA, Av. 11 de Septiembre 4755, 5020, Córdoba, Argentina. nicobejerman@gmail.com.
[Ti] Título:Genome characterization of an Argentinean isolate of alfalfa leaf curl virus.
[So] Source:Arch Virol;163(3):799-803, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:We investigated the molecular characteristics of an Argentinean isolate of alfalfa leaf curl virus (ALCV-Arg), a virus of the genus Capulavirus in the family Geminiviridae that was isolated from alfalfa plants showing dwarfism. The genome was found to be 2,750 nucleotides in length. In pairwise comparisons, this ALCV isolate shared 83.2% to 92.6% sequence identity with European ALCV isolates. Sequence comparisons and phylogenetic analysis showed that this isolate combines features of strains A and B of ALCV. Recombination analysis showed that ALCV-Arg is a recombinant isolate that was generated by intraspecific recombination between ALCV strains A and B. The results of this study not only show that ALCV-Arg is unique because it combines features of strains A and B but also show that ALCV naturally infects this forage crop on the American continent.
[Mh] Termos MeSH primário: DNA Viral/genética
Geminiviridae/genética
Genoma Viral
Medicago sativa/virologia
Filogenia
Folhas de Planta/virologia
[Mh] Termos MeSH secundário: Argentina
Sequência de Bases
Geminiviridae/classificação
Geminiviridae/isolamento & purificação
Sequenciamento de Nucleotídeos em Larga Escala
Doenças das Plantas/virologia
Recombinação Genética
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3673-x


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[PMID]:29196817
[Au] Autor:Perotto MC; Pozzi EA; Celli MG; Luciani CE; Mitidieri MS; Conci VC
[Ad] Endereço:Instituto de Patología Vegetal (IPAVE-CIAP-INTA), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina. perotto.cecilia@inta.gob.ar.
[Ti] Título:Identification and characterization of a new potyvirus infecting cucurbits.
[So] Source:Arch Virol;163(3):719-724, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
[Mh] Termos MeSH primário: Cucurbita/virologia
Genoma Viral
Filogenia
Potyvirus/genética
RNA Viral/genética
[Mh] Termos MeSH secundário: Argentina
Sequência de Bases
Sequenciamento de Nucleotídeos em Larga Escala
Fases de Leitura Aberta
Doenças das Plantas/virologia
Potyvirus/classificação
Potyvirus/isolamento & purificação
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3660-2


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[PMID]:28455950
[Au] Autor:Koch S; Schulz TF
[Ad] Endereço:.
[Ti] Título:Rhadinoviral interferon regulatory factor homologues.
[So] Source:Biol Chem;398(8):857-870, 2017 07 26.
[Is] ISSN:1437-4315
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8) is a gammaherpesvirus and the etiological agent of Kaposi's sarcoma, primary effusion lymphoma and multicentric Castleman disease. The KSHV genome contains genes for a unique group of proteins with homology to cellular interferon regulatory factors, termed viral interferon regulatory factors (vIRFs). This review will give an overview over the oncogenic, antiapoptotic and immunomodulatory characteristics of KSHV and related vIRFs.
[Mh] Termos MeSH primário: Herpesvirus Humano 8
Fatores Reguladores de Interferon
Homologia de Sequência do Ácido Nucleico
Proteínas Virais
[Mh] Termos MeSH secundário: Animais
Herpesvirus Humano 8/genética
Herpesvirus Humano 8/metabolismo
Seres Humanos
Fatores Reguladores de Interferon/genética
Fatores Reguladores de Interferon/metabolismo
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Interferon Regulatory Factors); 0 (Viral Proteins); 0 (viral interferon regulatory factors)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29300775
[Au] Autor:Varughese LR; Rajpoot M; Goyal S; Mehra R; Chhokar V; Beniwal V
[Ad] Endereço:Department of Biotechnology, Maharishi Markandeshwar University, Mullana, Ambala, India.
[Ti] Título:Analytical profiling of mutations in quinolone resistance determining region of gyrA gene among UPEC.
[So] Source:PLoS One;13(1):e0190729, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mutations in gyrA are the primary cause of quinolone resistance encountered in gram-negative clinical isolates. The prospect of this work was to analyze the role of gyrA mutations in eliciting high quinolone resistance in uropathogenic E.coli (UPEC) through molecular docking studies. Quinolone susceptibility testing of 18 E.coli strains isolated from UTI patients revealed unusually high resistance level to all the quinolones used; especially norfloxacin and ciprofloxacin. The QRDR of gyrA was amplified and sequenced. Mutations identified in gyrA of E.coli included Ser83Leu, Asp87Asn and Ala93Gly/Glu. Contrasting previous reports, we found Ser83Leu substitution in sensitive strains. Strains with S83L, D87N and A93E (A15 and A26) demonstrated norfloxacin MICs ≥1024mg/L which could be proof that Asp87Asn is necessary for resistance phenotype. Resistance to levofloxacin was comparatively lower in all the isolates. Docking of 4 quinolones (ciprofloxacin, ofloxacin, levofloxacin and norfloxacin) to normal and mutated E.coli gyrase A protein demonstrated lower binding energies for the latter, with significant displacement of norfloxacin in the mutated GyrA complex and least displacement in case of levofloxacin.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
DNA Girase/genética
Farmacorresistência Bacteriana/genética
Proteínas de Escherichia coli/genética
Quinolonas/farmacologia
Escherichia coli Uropatogênica/genética
[Mh] Termos MeSH secundário: DNA Girase/metabolismo
Infecções por Escherichia coli/microbiologia
Proteínas de Escherichia coli/metabolismo
Seres Humanos
Testes de Sensibilidade Microbiana
Simulação de Acoplamento Molecular
Mutação
Homologia de Sequência do Ácido Nucleico
Infecções Urinárias/microbiologia
Escherichia coli Uropatogênica/efeitos dos fármacos
Escherichia coli Uropatogênica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Escherichia coli Proteins); 0 (Quinolones); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190729


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[PMID]:29244187
[Au] Autor:Salinas-Giegé T; Cavaiuolo M; Cognat V; Ubrig E; Remacle C; Duchêne AM; Vallon O; Maréchal-Drouard L
[Ad] Endereço:Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67084 Strasbourg, France.
[Ti] Título:Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii.
[So] Source:Nucleic Acids Res;45(22):12963-12973, 2017 Dec 15.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The unicellular photosynthetic organism, Chlamydomonas reinhardtii, represents a powerful model to study mitochondrial gene expression. Here, we show that the 5'- and 3'-extremities of the eight Chlamydomonas mitochondrial mRNAs present two unusual characteristics. First, all mRNAs start primarily at the AUG initiation codon of the coding sequence which is often marked by a cluster of small RNAs. Second, unusual tails are added post-transcriptionally at the 3'-extremity of all mRNAs. The nucleotide composition of the tails is distinct from that described in any other systems and can be partitioned between A/U-rich tails, predominantly composed of Adenosine and Uridine, and C-rich tails composed mostly of Cytidine. Based on 3' RACE experiments, 22% of mRNAs present C-rich tails, some of them composed of up to 20 consecutive Cs. Polycytidylation is specific to mitochondria and occurs primarily on mRNAs. This unprecedented post-transcriptional modification seems to be a specific feature of the Chlorophyceae class of green algae and points out the existence of novel strategies in mitochondrial gene expression.
[Mh] Termos MeSH primário: Chlamydomonas reinhardtii/genética
Mitocôndrias/genética
RNA Mensageiro/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Sequência de Bases
Chlamydomonas reinhardtii/metabolismo
Clorófitas/classificação
Clorófitas/genética
Genoma Mitocondrial/genética
Mitocôndrias/metabolismo
Filogenia
Poli C/metabolismo
RNA Mensageiro/metabolismo
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (mitochondrial messenger RNA); 30811-80-4 (Poly C)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx903



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