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  1 / 63526 MEDLINE  
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[PMID]:29367490
[Au] Autor:Asakawa T; Takano Y; Ohta A; Asakawa H
[Ad] Endereço:School of Chemistry, College of Science and Engineering, Kanazawa University.
[Ti] Título:Aggregation and pH Responsive Behavior of Thioester Surfactants and Formation of Disulfide Linkages in Aqueous Solutions.
[So] Source:J Oleo Sci;67(2):199-206, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:pH responsive surfactants, [C H N(CH ) (CH ) SCOCH ]Br (C nSAc, n = 4, 11, 12), were prepared, and their properties in aqueous solution were examined. The critical micelle concentration (cmc) and critical vesicle concentration (cvc) were determined based on changes in conductivity, as well as by fluorescence measurements, and light scattering methods. A significant increase in the light scattering intensities of the C nSAc (n=11, 12) systems suggested that the growth of aggregates was accompanied by considerable counterion binding with increasing surfactant concentration. The diameter of C 11SAc, recorded by the dynamic light scattering measurements, was about 9.6 ±1.0 nm, which was slightly smaller than that for didodecyldimethylammonium bromide (DDAB) vesicles. The thioester group was easily hydrolyzed upon the addition of NaOH, while it was hardly hydrolyzed with the addition of HCl. The time course of alkaline hydrolysis was examined by the conductivity measurements and high-performance liquid chromatography analysis. [C H N(CH ) (CH ) SS(CH ) N(CH ) C H ]2Br (2C 11SS) was generated in the C 11SAc alkaline solution because of air oxidation. The C 11SAc alkaline solution gradually became an opaque blue color with increasing light scattering at 346 nm, indicating the remarkable growth of vesicles. The chemical structure of 2C 11SS was consistent with that of a disulfide linked double tailed surfactant, similar to DDAB. The disulfide linkage between the double tailed surfactants will contribute to the stabilization and growth of vesicles.
[Mh] Termos MeSH primário: Ésteres/química
Tensoativos/química
Água/química
[Mh] Termos MeSH secundário: Dissulfetos/química
Difusão Dinâmica da Luz
Concentração de Íons de Hidrogênio
Hidrólise
Micelas
Oxirredução
Compostos de Amônio Quaternário/química
Soluções
Tensão Superficial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Esters); 0 (Micelles); 0 (Quaternary Ammonium Compounds); 0 (Solutions); 0 (Surface-Active Agents); 059QF0KO0R (Water); 13146-86-6 (didodecyldimethylammonium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17192


  2 / 63526 MEDLINE  
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[PMID]:28455252
[Au] Autor:Kwak HW; Shin M; Lee JY; Yun H; Song DW; Yang Y; Shin BS; Park YH; Lee KH
[Ad] Endereço:Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
[Ti] Título:Fabrication of an ultrafine fish gelatin nanofibrous web from an aqueous solution by electrospinning.
[So] Source:Int J Biol Macromol;102:1092-1103, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Electrospinning of aqueous gelatin solution obtained from bovine or porcine sources has been difficult to achieve without additional facilities, such as a temperature control oven or heating cover. Gelatin from cold-water fish has low contents of proline (Pro) and hydroxyproline (Hyp) compared with mammalian-derived gelatin. For this reason, the fish-derived gelatin maintains a sol state without showing gelation behavior at room temperature. In the present study, we prepared an ultrafine fish gelatin nanofibrous web by electrospinning from aqueous solutions without any additive polymers or temperature control facilities. The concentration and viscosity of fish gelatin are the most important factor in determining the electrospinnability and fiber diameter. Electrospinning of aqueous fish gelatin has the highest nanofiber productivity compared to other organic solvent systems. Using glutaraldehyde vapor (GTA), the water stability was improved and substantial enhancement was achieved in the mechanical properties. Finally, the cytotoxicity of a fish gelatin nanofibrous scaffold was evaluated based on a cell proliferation study by culturing human dermal fibroblasts (HDFs) compared with a fish gelatin film and nanofibrous mat from mammalian gelatin. The result shows better initial cell attachment and proliferation compared with the fish gelatin film and no significant difference compared with mammalian-derived gelatin nanofibrous mat. We expect that electrospinning of aqueous fish gelatin could be an effective alternative mammalian gelatin source.
[Mh] Termos MeSH primário: Eletricidade
Peixes
Gelatina/química
Nanofibras/química
Nanotecnologia
Água/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/química
Materiais Biocompatíveis/farmacologia
Bovinos
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Gelatina/farmacologia
Glutaral/química
Seres Humanos
Hidrólise
Reologia
Soluções
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Solutions); 059QF0KO0R (Water); 9000-70-8 (Gelatin); T3C89M417N (Glutaral)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  3 / 63526 MEDLINE  
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[PMID]:29441920
[Ti] Título:Pancreatic lipase and -amylase inhibitory activities of plants used in Traditional Chinese Medicine (TCM).
[So] Source:Pharmazie;71(7):420-424, 2016 Jul 07.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:To find new, plant based drugs for the treatment of obesity and/or diabetes mellitus type 2 through the inhibition of essential digestive enzymes, in vitro tests were carried out on selected plants or fungi with weight-reducing, blood glucose-reducing or related potential, used in Traditional Chinese Medicine (TCM). Aqueous and methanolic extracts of 32 Chinese herbal medicines were assayed for their in vitro inhibitory activity against pancreatic lipase (PL) and α-amylase (PA). PL activity was measured by using an enzymatic in vitro assay based on the hydrolysis kinetics of an oleate ester of 4-methylumbelliferone. For the determination of α-amylase activity an enzyme assay based on the hydrolytic cleavage of a modified starch derivative was used. Our findings have shown that the methanolic extract of Lycopus lucidus Turcz. var. hirtus Regel (Lamiaceae) was a very effective PL inhibitor (IC50: 88.3±4.1 µg/mL). A high anti-amylase activity showed the methanolic extract of Trichosanthes kirilowii Maxim. (Curcurbitaceae, IC50: 248.8±67.3 µg/mL). This work provides a priority list of interesting plants for further study with respect to the treatment of obesity and associated metabolic diseases.
[Mh] Termos MeSH primário: Lipase/antagonistas & inibidores
Pâncreas/enzimologia
Plantas/química
alfa-Amilases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Fungos/química
Hidrólise
Himecromona/química
Cinética
Lycopus/química
Medicina Tradicional Chinesa
Pâncreas/efeitos dos fármacos
Extratos Vegetais/farmacologia
Trichosanthes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 3T5NG4Q468 (Hymecromone); EC 3.1.1.3 (Lipase); EC 3.2.1.1 (alpha-Amylases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6569


  4 / 63526 MEDLINE  
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[PMID]:29194020
[Au] Autor:Zhang J; Yuan J; Zhang WX; Tu F; Jiang Y; Sun CZ
[Ad] Endereço:a Department of Life Science and Food Engineering/Key Laboratory of Fermentation Resource and Application in Sichuan/Department of Library , Yibin University , Yibin , Sichuan , China.
[Ti] Título:Anaerobic detoxification fermentation by Rhodospirillum rubrum for rice straw as feed with moderate pretreatment.
[So] Source:Prep Biochem Biotechnol;48(1):75-83, 2018 Jan 02.
[Is] ISSN:1532-2297
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel and effective process was put forward for converting rice straw into feed by combining diluted acid hydrolysis and ammonization with Rhodospirillum rubrum fermentation. After pretreatment with dilute sulfuric or phosphoric acid (1%, w/w) at 100°C, materials were subjected to fermentation under several gases (N , CO , and air) and different light intensities in a 2-L fermentor. The key indexes of feed for fermented materials were estimated and several toxic substances were investigated during the fermentation. Following sulfuric acid treatment, the true protein of rice straw increased from 29 to 143 g kg and the crude fiber decreased from 359 to 136 g kg after fermentation at 0.3 L min L of N flow and a light intensity of 3400 lux; and following phosphoric acid treatment, the true protein increased by 286% and the crude fiber decreased by 52% after fermentation at 0.4 L min L of N flow and a light intensity of 3000 lux. Other key contents were also improved for use as feed, and some toxic substances (i.e., furfural, hydroxymethylfurfural, acetic acid, phenol, cresol) produced by the pretreatments could be removed at low levels during the fermentations.
[Mh] Termos MeSH primário: Ração Animal/análise
Oryza/metabolismo
Rhodospirillum rubrum/metabolismo
[Mh] Termos MeSH secundário: Ração Animal/toxicidade
Fermentação
Hidrólise
Microbiologia Industrial
Luz
Ácidos Fosfóricos/metabolismo
Ácidos Sulfúricos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphoric Acids); 0 (Sulfuric Acids); E4GA8884NN (phosphoric acid); O40UQP6WCF (sulfuric acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1080/10826068.2017.1405023


  5 / 63526 MEDLINE  
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[PMID]:29339722
[Au] Autor:Sousa DZ; Visser M; van Gelder AH; Boeren S; Pieterse MM; Pinkse MWH; Verhaert PDEM; Vogt C; Franke S; Kümmel S; Stams AJM
[Ad] Endereço:Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
[Ti] Título:The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways.
[So] Source:Nat Commun;9(1):239, 2018 01 16.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17 , isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.
[Mh] Termos MeSH primário: Álcool Desidrogenase/metabolismo
Proteínas de Bactérias/metabolismo
Desulfotomaculum/enzimologia
Redes e Vias Metabólicas/genética
Metanol/metabolismo
Metiltransferases/metabolismo
[Mh] Termos MeSH secundário: Álcool Desidrogenase/genética
Proteínas de Bactérias/genética
Cobalto/metabolismo
Cobalto/farmacologia
Meios de Cultura/química
Desulfotomaculum/genética
Expressão Gênica
Perfilação da Expressão Gênica
Hidrólise
Metiltransferases/genética
Oxirredução
Filogenia
Proteômica/métodos
Vitamina B 12/metabolismo
Vitamina B 12/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 3G0H8C9362 (Cobalt); EC 1.1.1.1 (Alcohol Dehydrogenase); EC 2.1.1.- (Methyltransferases); EVS87XF13W (cobaltous chloride); P6YC3EG204 (Vitamin B 12); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02518-9


  6 / 63526 MEDLINE  
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[PMID]:29295984
[Au] Autor:Goyal N; Rossi MJ; Mazina OM; Chi Y; Moritz RL; Clurman BE; Mazin AV
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, 19102, USA.
[Ti] Título:RAD54 N-terminal domain is a DNA sensor that couples ATP hydrolysis with branch migration of Holliday junctions.
[So] Source:Nat Commun;9(1):34, 2018 01 02.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In eukaryotes, RAD54 catalyzes branch migration (BM) of Holliday junctions, a basic process during DNA repair, replication, and recombination. RAD54 also stimulates RAD51 recombinase and has other activities. Here, we investigate the structural determinants for different RAD54 activities. We find that the RAD54 N-terminal domain (NTD) is responsible for initiation of BM through two coupled, but distinct steps; specific binding to Holliday junctions and RAD54 oligomerization. Furthermore, we find that the RAD54 oligomeric state can be controlled by NTD phosphorylation at S49, a CDK2 consensus site, which inhibits RAD54 oligomerization and, consequently, BM. Importantly, the effect of phosphorylation on RAD54 oligomerization is specific for BM, as it does not affect stimulation of RAD51 recombinase by RAD54. Thus, the transition of the oligomeric states provides an important control of the biological functions of RAD54 and, likely, other multifunctional proteins.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/metabolismo
DNA Helicases/metabolismo
DNA Cruciforme/metabolismo
Proteínas Nucleares/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sítios de Ligação/genética
Linhagem Celular
DNA Helicases/química
DNA Helicases/genética
Reparo do DNA
DNA Cruciforme/química
DNA Cruciforme/genética
Seres Humanos
Hidrólise
Proteínas Nucleares/química
Proteínas Nucleares/genética
Conformação de Ácido Nucleico
Fosforilação
Multimerização Proteica
Recombinação Genética
Homologia de Sequência de Aminoácidos
Células Sf9
Spodoptera
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Cruciform); 0 (Nuclear Proteins); 0 (RAD54L protein, human); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.4.- (DNA Helicases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02497-x


  7 / 63526 MEDLINE  
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[PMID]:28464292
[Au] Autor:Charbgoo F; Behmanesh M; Nikkhah M; Kane EG
[Ad] Endereço:Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
[Ti] Título:RNAi mediated gene silencing of ITPA using a targeted nanocarrier: Apoptosis induction in SKBR3 cancer cells.
[So] Source:Clin Exp Pharmacol Physiol;44(8):888-894, 2017 Aug.
[Is] ISSN:1440-1681
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:A pure nucleotide pool is required for high-fidelity DNA replication and prevention of carcinogenesis in living cells. Human inosine triphosphatase (ITPase), encoded by the ITPA gene, plays a critical role in maintaining the purity of the cellular nucleotide pool by excluding nucleotides that enhance mutagenesis. ITPase is a nucleoside triphosphate pyrophosphatase that hydrolyzes the non-canonical nucleotides inosine triphosphate (ITP) and xanthine triphosphate (XTP). The monophosphate products of ITPase reactions are subsequently excluded from the nucleotide pool and the improper substitution of ITP and XTP into DNA and RNA is prevented. Previous studies show that deficiency in ITPA can suppress cellular growth and enhance DNA instability. In this study, we evaluated the influence of effective ITPA down-regulation on the induction of apoptosis in a human cancer cell line using folate-single wall nanotubes (SWNT) as a targeted nanocarrier. We assessed whether SWNT enhances IPTA-siRNA transfection efficiency in cancer cells using folate as a homing device. Since folate receptor is considerably overexpressed in cancer cells, conjugation of SWNTs to folate could enhance their cancer-specific penetrance. We found that nanocarrier mediated ITPA-siRNA transfection into SKBR3 cells caused significant reduction of ITPA mRNA expression level and complete down-regulation of the ITPase protein product. The silencing of ITPA led to promotion of apoptosis in SWNT-treated SKBR3 cancer cells.
[Mh] Termos MeSH primário: Apoptose/genética
Portadores de Fármacos/química
Nanoestruturas/química
Nanotubos de Carbono/química
Pirofosfatases/deficiência
Pirofosfatases/genética
Interferência de RNA
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Regulação para Baixo/genética
Ácido Fólico/química
Seres Humanos
Hidrólise
RNA Interferente Pequeno/química
RNA Interferente Pequeno/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Nanotubes, Carbon); 0 (RNA, Small Interfering); 935E97BOY8 (Folic Acid); EC 3.6.1.- (Pyrophosphatases); EC 3.6.1.19 (ITPA protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/1440-1681.12776


  8 / 63526 MEDLINE  
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[PMID]:28457955
[Au] Autor:Tapadiya A; Vasanthan N
[Ad] Endereço:Department of Chemistry and Biochemistry, Long Island University, One University Plaza, Brooklyn, NY 11201, United States.
[Ti] Título:Crystallization and alkaline hydrolysis of poly(3- hydroxybutyrate) films probed by thermal analysis and infrared spectroscopy.
[So] Source:Int J Biol Macromol;102:1130-1137, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Poly(3-hydroxybutyrate) (PHB) is a microbially synthesized polymer, which is often purified by alkaline treatment. The effect of microstructure on alkaline hydrolysis has been studied by varying concentration of base and the temperature. The morphologies of PHB films before and after degradation were evaluated using DSC and FTIR spectroscopy. The hydrolytic degradation study by weight loss measurement revealed that the crystallinity of PHB greatly decreased the hydrolytic ability of PHB. The crystallization of PHB and the effect of base on hydrolysis was investigated by time dependent FTIR spectroscopy. The normalized absorbance of 3010cm and 1183cm were used to characterize the crystalline and the amorphous phases of PHB. FTIR spectroscopy reveal that the extent of hydrolysis decreased with increasing crystallinity. The crotonic acid was detected as a major product after hydrolysis, confirmed by UV/Visible and proton NMR spectroscopy. The normalized absorbance of the crystalline band at 3010cm band remained constant, suggesting that there is no significant change in crystallinity with degradation. The normalized amorphous band at 1183cm showed a decrease in absorbance ratio, suggesting degradation of the amorphous phase. Our data suggests that alkaline hydrolysis depends on concentration of base and the crystallinity of PHB.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/química
Temperatura Ambiente
[Mh] Termos MeSH secundário: Cristalização
Concentração de Íons de Hidrogênio
Hidrólise
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  9 / 63526 MEDLINE  
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[PMID]:29329090
[Au] Autor:Mantovani CC; Silva JPE; Forster G; Almeida RM; Diniz EMA; Yonamine M
[Ad] Endereço:Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, 13B, 05508-900 São Paulo, SP, Brazil.
[Ti] Título:Simultaneous accelerated solvent extraction and hydrolysis of 11-nor-Δ -tetrahydrocannabinol-9-carboxylic acid glucuronide in meconium samples for gas chromatography-mass spectrometry analysis.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:1-7, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal-fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography-mass spectrometry (GC-MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were <8.4% for all concentration levels. Accuracy was in 101.7-108.9% range. Recovery was on average 60.3%. Carryover effect was not observed. The procedure was applied in six meconium samples from babies whose mothers were drug users and showed satisfactory performance to confirm fetal cannabis exposure.
[Mh] Termos MeSH primário: Dronabinol/análogos & derivados
Cromatografia Gasosa-Espectrometria de Massas/métodos
Mecônio/química
[Mh] Termos MeSH secundário: Dronabinol/análise
Dronabinol/química
Dronabinol/isolamento & purificação
Dronabinol/metabolismo
Feminino
Glucuronídeos
Seres Humanos
Hidrólise
Recém-Nascido
Modelos Lineares
Abuso de Maconha/metabolismo
Gravidez
Complicações na Gravidez/metabolismo
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucuronides); 4TPC9E4A32 (11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid); 7J8897W37S (Dronabinol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  10 / 63526 MEDLINE  
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[PMID]:29311594
[Au] Autor:Nakanishi A; Kishikawa JI; Tamakoshi M; Mitsuoka K; Yokoyama K
[Ad] Endereço:Department of Molecular Biosciences, Kyoto Sangyo University, Motoyama Kamigamo, Kita-ku, Kyoto, 603-8555, Japan.
[Ti] Título:Cryo EM structure of intact rotary H -ATPase/synthase from Thermus thermophilus.
[So] Source:Nat Commun;9(1):89, 2018 01 08.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Proton translocating rotary ATPases couple ATP hydrolysis/synthesis, which occurs in the soluble domain, with proton flow through the membrane domain via a rotation of the common central rotor complex against the surrounding peripheral stator apparatus. Here, we present a large data set of single particle cryo-electron micrograph images of the V/A type H -rotary ATPase from the bacterium Thermus thermophilus, enabling the identification of three rotational states based on the orientation of the rotor subunit. Using masked refinement and classification with signal subtractions, we obtain homogeneous reconstructions for the whole complexes and soluble V domains. These reconstructions are of higher resolution than any EM map of intact rotary ATPase reported previously, providing a detailed molecular basis for how the rotary ATPase maintains structural integrity of the peripheral stator apparatus, and confirming the existence of a clear proton translocation path from both sides of the membrane.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Proteínas de Bactérias/metabolismo
Thermus thermophilus/enzimologia
ATPases Vacuolares Próton-Translocadoras/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/ultraestrutura
Transporte Biológico
Microscopia Crioeletrônica
Hidrólise
Modelos Moleculares
Conformação Proteica
Subunidades Proteicas/química
Subunidades Proteicas/metabolismo
Prótons
Rotação
ATPases Vacuolares Próton-Translocadoras/química
ATPases Vacuolares Próton-Translocadoras/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Protein Subunits); 0 (Protons); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02553-6



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