Base de dados : MEDLINE
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[PMID]:29346419
[Au] Autor:Holcomb J; Doughan M; Spellmon N; Lewis B; Perry E; Zhang Y; Nico L; Wan J; Chakravarthy S; Shang W; Miao Q; Stemmler T; Yang Z
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan, United States of America.
[Ti] Título:SAXS analysis of a soluble cytosolic NgBR construct including extracellular and transmembrane domains.
[So] Source:PLoS One;13(1):e0191371, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Nogo-B receptor (NgBR) is involved in oncogenic Ras signaling through directly binding to farnesylated Ras. It recruits farnesylated Ras to the non-lipid-raft membrane for interaction with downstream effectors. However, the cytosolic domain of NgBR itself is only partially folded. The lack of several conserved secondary structural elements makes this domain unlikely to form a complete farnesyl binding pocket. We find that inclusion of the extracellular and transmembrane domains that contain additional conserved residues to the cytosolic region results in a well folded protein with a similar size and shape to the E.coli cis-isoprenyl transferase (UPPs). Small Angle X-ray Scattering (SAXS) analysis reveals the radius of gyration (Rg) of our NgBR construct to be 18.2 Å with a maximum particle dimension (Dmax) of 61.0 Å. Ab initio shape modeling returns a globular molecular envelope with an estimated molecular weight of 23.0 kD closely correlated with the calculated molecular weight. Both Kratky plot and pair distribution function of NgBR scattering reveal a bell shaped peak which is characteristic of a single globularly folded protein. In addition, circular dichroism (CD) analysis reveals that our construct has the secondary structure contents similar to the UPPs. However, this result does not agree with the currently accepted topological orientation of NgBR which might partition this construct into three separate domains. This discrepancy suggests another possible NgBR topology and lends insight into a potential molecular basis of how NgBR facilitates farnesylated Ras recruitment.
[Mh] Termos MeSH primário: Receptores de Superfície Celular/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação
Membrana Celular/metabolismo
Dicroísmo Circular
Citosol/metabolismo
Peso Molecular
Estrutura Secundária de Proteína
Receptores de Superfície Celular/metabolismo
Espalhamento a Baixo Ângulo
Homologia de Sequência de Aminoácidos
Solubilidade
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (NUS1 protein, human); 0 (Receptors, Cell Surface)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191371


  2 / 149094 MEDLINE  
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[PMID]:28465177
[Au] Autor:Liu NN; Chi Z; Wang QQ; Hong J; Liu GL; Hu Z; Chi ZM
[Ad] Endereço:College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
[Ti] Título:Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
[So] Source:Int J Biol Macromol;102:1016-1024, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×10 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×10 and a pullulan titer was 65.3g/L. The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase. During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×10 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×10 . Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5. Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
[Mh] Termos MeSH primário: Ascomicetos/isolamento & purificação
Ascomicetos/metabolismo
Glucanos/biossíntese
Glucanos/química
Oligossacarídeos/biossíntese
Oligossacarídeos/química
Zonas Úmidas
[Mh] Termos MeSH secundário: Fermentação
Peso Molecular
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Oligosaccharides); 8ZQ0AYU1TT (pullulan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 149094 MEDLINE  
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[PMID]:28461165
[Au] Autor:Ahmed FRS; Amin R; Hasan I; Asaduzzaman AKM; Kabir SR
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh.
[Ti] Título:Antitumor properties of a methyl-ß-d-galactopyranoside specific lectin from Kaempferia rotunda against Ehrlich ascites carcinoma cells.
[So] Source:Int J Biol Macromol;102:952-959, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A lectin was isolated from the tuberous rhizome of Keampferia rotunda by using different chromatographic methods with the molecular weight of 21±1kDa. The lectin contained highest percentage of leucine and lowest percentage of tryptophan residues as determined by LC-MS. The lectin agglutinated mice and human erythrocytes and the hemagglutination activity was inhibited by Methyl-ß-d-galactopyranoside. The lectin did not lose its activity in the presence of urea but the activity abolished completely when treated with EDTA. The lectin exhibited its activity at the pH ranging from 6.0 to 9.0 and in a temperature range of 30-80°C. Antiproliferative activity was studied against Ehrlich ascites carcinoma (EAC) and U87 cell lines. No inhibitory effect was observed against U87 cell line whereas 43.7% cell growth inhibition was observed in vitro against EAC cells at 160µg/ml. The lectin was injected (i.p.) in EAC bearing Swiss albino mice at the doses of 3.0 and 6.0mg/kg/day for five consecutive days and 41 and 59% of EAC cell growth inhibition was observed, respectively. The cell growth inhibition was due to the induction of apoptosis in the EAC cells which was confirmed by cell morphological study, caspase-3 inhibitor and apoptosis-related gene expression.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Carcinoma de Ehrlich/patologia
Galactose/metabolismo
Lectinas de Plantas/farmacologia
Zingiberaceae/química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Antineoplásicos/metabolismo
Apoptose/efeitos dos fármacos
Apoptose/genética
Caspases/metabolismo
Bovinos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Hemaglutinação/efeitos dos fármacos
Seres Humanos
Concentração de Íons de Hidrogênio
Camundongos
Peso Molecular
Lectinas de Plantas/química
Lectinas de Plantas/isolamento & purificação
Lectinas de Plantas/metabolismo
Especificidade por Substrato
Temperatura Ambiente
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Lectins); EC 3.4.22.- (Caspases); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28453668
[Au] Autor:Kou Q; Wu S; Tolic N; Pasa-Tolic L; Liu Y; Liu X
[Ad] Endereço:Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA.
[Ti] Título:A mass graph-based approach for the identification of modified proteoforms using top-down tandem mass spectra.
[So] Source:Bioinformatics;33(9):1309-1316, 2017 05 01.
[Is] ISSN:1367-4811
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Motivation: Although proteomics has rapidly developed in the past decade, researchers are still in the early stage of exploring the world of complex proteoforms, which are protein products with various primary structure alterations resulting from gene mutations, alternative splicing, post-translational modifications, and other biological processes. Proteoform identification is essential to mapping proteoforms to their biological functions as well as discovering novel proteoforms and new protein functions. Top-down mass spectrometry is the method of choice for identifying complex proteoforms because it provides a 'bird's eye view' of intact proteoforms. The combinatorial explosion of various alterations on a protein may result in billions of possible proteoforms, making proteoform identification a challenging computational problem. Results: We propose a new data structure, called the mass graph, for efficient representation of proteoforms and design mass graph alignment algorithms. We developed TopMG, a mass graph-based software tool for proteoform identification by top-down mass spectrometry. Experiments on top-down mass spectrometry datasets showed that TopMG outperformed existing methods in identifying complex proteoforms. Availability and implementation: http://proteomics.informatics.iupui.edu/software/topmg/. Contact: xwliu@iupui.edu. Supplementary information: Supplementary data are available at Bioinformatics online.
[Mh] Termos MeSH primário: Proteoma/análise
Proteômica/métodos
Software
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Algoritmos
Processamento Alternativo
Peso Molecular
Mutação
Processamento de Proteína Pós-Traducional
Proteoma/química
Proteoma/genética
Proteoma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Proteome)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/bioinformatics/btw806


  5 / 149094 MEDLINE  
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[PMID]:28465009
[Au] Autor:Dong G; Kalifa R; Nath PR; Babichev Y; Gelkop S; Isakov N
[Ad] Endereço:The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences and the Cancer Research Center, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel. Electronic address: gdong@upenn.edu.
[Ti] Título:Crk adaptor proteins regulate CD3ζ chain phosphorylation and TCR/CD3 down-modulation in activated T cells.
[So] Source:Cell Signal;36:117-126, 2017 Aug.
[Is] ISSN:1873-3913
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:T cell receptor (TCR) recognition of a peptide antigen in the context of MHC molecules initiates positive and negative cascades that regulate T cell activation, proliferation and differentiation, and culminate in the acquisition of effector T cell functions. These processes are a prerequisite for the induction of specific T cell-mediated adaptive immune responses. A key event in the activation of TCR-coupled signaling pathways is the phosphorylation of tyrosine residues within the cytoplasmic tails of the CD3 subunits, predominantly CD3ζ. These transiently formed phosphotyrosyl epitopes serve as docking sites for SH2-domain containing effector molecules, predominantly the ZAP70 protein tyrosine kinase, which is critical for signal propagation. We found that CrkI and CrkII adaptor proteins also interact with CD3ζ in TCR activated-, but not in resting-, T cells. Crk binding to CD3ζ was independent of ZAP70 and also occurred in ZAP70-deficient T cells. Binding was mediated by Crk-SH2 domain interaction with phosphotyrosine-containing motifs on CD3ζ, via a direct physical interaction, as demonstrated by Far-Western blot. CrkII binding to CD3ζ could also be demonstrated in a heterologous system, where coexpression of a catalytically active Lck was used to phosphorylate the CD3ζ chain. TCR activation-induced Crk binding to CD3ζ resulted in increased and prolonged phosphorylation of CD3ζ, as well as ZAP70 and LAT, suggesting a positive role for CrkI/II binding to CD3ζ in regulation of TCR-coupled signaling pathways. Furthermore, Crk-dependent increased phosphorylation of CD3ζ coincided with inhibition of TCR downmodulation, supporting a positive role for Crk adaptor proteins in TCR-mediated signal amplification.
[Mh] Termos MeSH primário: Complexo CD3/metabolismo
Regulação para Baixo
Ativação Linfocitária
Proteínas Proto-Oncogênicas c-crk/metabolismo
Receptores de Antígenos de Linfócitos T/metabolismo
Linfócitos T/metabolismo
[Mh] Termos MeSH secundário: Animais
Anticorpos/metabolismo
Células COS
Cercopithecus aethiops
Reagentes para Ligações Cruzadas/farmacologia
Regulação para Baixo/efeitos dos fármacos
Seres Humanos
Células Jurkat
Ativação Linfocitária/efeitos dos fármacos
Camundongos
Peso Molecular
Fosforilação/efeitos dos fármacos
Fosfotirosina/metabolismo
Ligação Proteica/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-crk/química
Linfócitos T/efeitos dos fármacos
Vanadatos/farmacologia
Proteína-Tirosina Quinase ZAP-70/metabolismo
Domínios de Homologia de src
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (CD3 Complex); 0 (CRK protein, human); 0 (Cross-Linking Reagents); 0 (Proto-Oncogene Proteins c-crk); 0 (Receptors, Antigen, T-Cell); 0 (pervanadate); 21820-51-9 (Phosphotyrosine); 3WHH0066W5 (Vanadates); EC 2.7.10.2 (ZAP-70 Protein-Tyrosine Kinase); EC 2.7.10.2 (ZAP70 protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:29024885
[Au] Autor:Jager D; Kupka D; Vaclavikova M; Ivanicova L; Gallios G
[Ad] Endereço:Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, 040 01, Kosice, Slovakia.
[Ti] Título:Degradation of Reactive Black 5 by electrochemical oxidation.
[So] Source:Chemosphere;190:405-416, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Degradation of commercial grade Reactive Black 5 (RB5) azo dye by chemical and electrochemical treatment was examined using a dimensionally stable anode and stainless steel cathodes as electrode materials, with NaCl as supporting electrolyte. The electrochemical treatment was compared to the chemical treatment with hypochlorite generated by electrolysis. The compounds present in the commercial grade RB5 azo dye and the products of its electrochemical degradation were separated using ion-pairing high performance liquid chromatography on reversed phase. The separated species were detected by diode array detector and electrospray ionization mass spectrometry. A suitable ion-pairing reversed phase HPLC-MS method with electrospray ionization for the separation and identification of the components was developed. The accurate mass of the parent and fragment ions were used in the determination of the empirical formulas of the components using the first-order mass spectra. Structural formulas of degradation products were proposed using these information and principles of organic chemistry and electrochemistry.
[Mh] Termos MeSH primário: Eletrólise/métodos
Naftalenossulfonatos/química
[Mh] Termos MeSH secundário: Compostos Azo/química
Cromatografia Líquida de Alta Pressão/métodos
Corantes/química
Estrutura Molecular
Peso Molecular
Oxirredução
Espectrometria de Massas por Ionização por Electrospray/métodos
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Coloring Agents); 0 (Naphthalenesulfonates); O0HDY58362 (Remazol black B)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE


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[PMID]:29192617
[Au] Autor:Vasilyeva IN; Bespalov VG; Semenov AL; Baranenko DA; Zinkin VN
[Ad] Endereço:Scientific Laboratory for Cancer Chemoprevention and Oncopharmacology at N.N. Petrov Research Institute of Oncology under the Ministry of Health of the Russian Federation, Moscow; International Research Centre "Biotechnologies of the Third Millennium", ITMO University, St. Petersburg, Russian Federa
[Ti] Título:The Effects of Low-Frequency Noise on Rats: Evidence of Chromosomal Aberrations in the Bone Marrow Cells and the Release of Low-Molecular-Weight DNA in the Blood Plasma.
[So] Source:Noise Health;19(87):79-83, 2017 Mar-Apr.
[Is] ISSN:1463-1741
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Evaluation of the effect of low-frequency noise (LFN) on the frequency of chromosomal aberrations in the bone marrow cells and on the content of low-molecular-weight DNA (lmwDNA) in the blood plasma of rats. MATERIALS AND METHODS: A total of 96 male Wistar rats were exposed to either single (17 min session) or multiple (17 min session repeated five times a week for 13 weeks) LFN, with the maximum range below 250 Hz and the sound pressure levels (SPLs) at 120 and 150 dB, respectively. The rats in the control groups were not subjected to any impact. The frequency of chromosomal aberrations in the bone marrow cells and the levels of lmwDNA in the blood plasma were measured afterwards. RESULTS: It has been detected that a single LFN exposure with either corresponding SPLs had a significant increase in the frequency of chromosomal aberrations (more than 10-fold) compared to the controls (0.9 ± 0.3%) and resulted in the appearance of dicentric chromosomes in the aberration spectrum, both of which are evident for the occurrence of deoxyribonucleic acid double strand breaks triggered by the exposure. Furthermore, the lmwDNA levels in the blood plasma measured the following day after a single LFN exposure were significantly higher (7.7- and 7.6-fold, respectively) than that in the control group (11.0 ± 5.4 ng/ml), and such levels were maintained higher (4.8- and 2.1-fold, respectively) in the week after a single LFN exposure for the SPL of 120 and 150 dB, respectively, compared to the control group (18.8 ± 1.6 ng/ml). Similar results were obtained from the group with multiple LFN exposures (36.4- and 22.4-fold, respectively) compared to the control (17.7 ± 1.7 ng/ml) and suggest the enhancement of cellular apoptosis as a result of the LFN impact. CONCLUSION: Presumably, the LFN may have possible mutagenic effects and cause massive cell death.
[Mh] Termos MeSH primário: Células da Medula Óssea/patologia
Aberrações Cromossômicas
DNA/sangue
Ruído/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Masculino
Peso Molecular
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.4103/nah.NAH_39_16


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[PMID]:29247504
[Au] Autor:Gallegos-Tabanico A; Sarabia-Sainz JA; Sarabia-Sainz HM; Carrillo Torres R; Guzman-Partida AM; Monfort GR; Silva-Campa E; Burgara-Estrella AJ; Angulo-Molina A; Acosta-Elias M; Pedroza-Montero M; Vazquez-Moreno L
[Ad] Endereço:Departamento de Física, Universidad de Sonora, Hermosillo, Sonora, 83000, México.
[Ti] Título:Molecular recognition of glyconanoparticles by RCA and E. coli K88 - designing transports for targeted therapy.
[So] Source:Acta Biochim Pol;64(4):671-677, 2017.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The targeted drug delivery has been studied as one of the main methods in medicine to ensure successful treatments of diseases. Pharmaceutical sciences are using micro or nano carriers to obtain a controlled delivery of drugs, able to selectively interact with pathogens, cells or tissues. In this work, we modified bovine serum albumin (BSA) with lactose, obtaining a neoglycan (BSA-Lac). Subsequently, we synthesized glyconanoparticles (NPBSA-Lac) with the premise that it would be recognized by microbial galactose specific lectins. NPBSA-Lac were tested for bio-recognition with adhesins of E. coli K88 and Ricinus communis agglutinin I (RCA). Glycation of BSA with lactose was analyzed by electrophoresis, infrared spectroscopy and fluorescence. Approximately 41 lactoses per BSA molecule were estimated. Nanoparticles were obtained using water in oil emulsion method and spheroid morphology with a range size of 300-500 nm was observed. Specific recognition of NPBSA-Lac by RCA and E. coli K88 was displayed by aggregation of nanoparticles analyzed by dynamic light scattering and atomic force microscopy. The results indicate that the lactosylated nanovectors could be targeted at the E. coli K88 adhesin and potentially could be used as a transporter for an antibacterial drug.
[Mh] Termos MeSH primário: Antígenos de Bactérias/metabolismo
Portadores de Fármacos/metabolismo
Proteínas de Escherichia coli/metabolismo
Proteínas de Fímbrias/metabolismo
Nanopartículas/química
Lectinas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Portadores de Fármacos/química
Eletroforese em Gel de Poliacrilamida
Escherichia coli/metabolismo
Lactose/química
Microscopia de Força Atômica
Peso Molecular
Tamanho da Partícula
Soroalbumina Bovina/química
Espectrofotometria Infravermelho
Espectroscopia de Infravermelho com Transformada de Fourier
Triptofano/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Drug Carriers); 0 (Escherichia coli Proteins); 0 (K88 antigen, E coli); 0 (Plant Lectins); 0 (Ricinus communis agglutinin-1); 147680-16-8 (Fimbriae Proteins); 27432CM55Q (Serum Albumin, Bovine); 8DUH1N11BX (Tryptophan); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2017_1639


  9 / 149094 MEDLINE  
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[PMID]:29287723
[Au] Autor:Yan J; Liang X; Cui Y; Cao X; Gao J
[Ad] Endereço:College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:Elovl4 can effectively elongate C18 polyunsaturated fatty acids in loach Misgurnus anguillicaudatus.
[So] Source:Biochem Biophys Res Commun;495(4):2637-2642, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, full-length cDNA sequences of elovl4a and elovl4b from loach Misgurnus anguillicaudatus were cloned. The full-length cDNAs of loach elovl4a and elovl4b were 2423 and 2054bp, encoding 315 and 300 amino acids, respectively. The deduced amino acid sequences of elovl4a and elovl4b in loach both shared the highest identity with those of Danio rerio, whereas lower identity score between loach elovl4a and elovl4b was present. Temporal expression and tissue expression of loach elovl4a and elovl4b were studied by reverse transcriptase PCR. Results of the tissue expression analyses suggested different functions of loach elovl4a and elovl4b. Functional characterizations of loach elovl4a and elovl4b on synthesis of fatty acids, especially elongating C18 polyunsaturated fatty acids (PUFAs) to longer-chain fatty acids, were studied by heterologous expression in Saccharomyces cerevisiae. Loach elovl4a and elvol4b enzymes were able to elongate all fatty acids tested including 18:2n-6, 18:3n-3, 18:3n-6, 20:4n-6 and 20:5n-3. At last, expression levels of the two elovl4 genes of loach fin cells incubated with 18:2n-6 and 18:3n-3 of different concentrations were measured. Expressions of elovl4a and elovl4b of loach fin cells were significantly up-regulated by 18:2n-6 and 18:3n-3. The results obtained here indicated that loach elovl4 could effectively elongate C18 PUFAs. This was a systematic report of elovl4's elongating functions towards C18 and provided an alternative pathway for C20 biosynthesis in fish species.
[Mh] Termos MeSH primário: Acetiltransferases/química
Acetiltransferases/metabolismo
Cipriniformes/metabolismo
Ácidos Graxos Insaturados/química
Ácidos Graxos Insaturados/metabolismo
Metabolismo dos Lipídeos/fisiologia
[Mh] Termos MeSH secundário: Animais
Peso Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids, Unsaturated); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (fatty acid elongases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:28461043
[Au] Autor:Pei J; Jiang L
[Ad] Endereço:Shaanxi Key Laboratory of Biology and Bioresources, Shaanxi University of Technology, Chaoyang Road, Hanzhong, Shaanxi 723001, China. Electronic address: jinjinpeislg@163.com.
[Ti] Título:Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.
[So] Source:Int J Antimicrob Agents;50(1):41-46, 2017 Jul.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Andrias davidianus, the Chinese giant salamander, has been used in traditional Chinese medicine for many decades. However, no antimicrobial peptides (AMPs) have been described from A. davidianus until now. Here we describe a novel AMP (andricin 01) isolated from the mucus of A. davidianus. The peptide was recovered using an innovative magnetic cell membrane separation technique and was characterised using mass spectrometry and circular dichroism (CD) spectroscopy. Andricin 01 is comprised of ten amino acid residues with a total molecular mass of 955.1 Da. CD spectrum analysis gave results similar to the archetypal random coil spectrum, consistent with the three-dimensional rendering calculated by current bioinformatics tools. Andricin 01 was found to be inhibitory both to Gram-negative and Gram-positive bacteria. Furthermore, the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use. Andricin 01 shows promise as a novel antibacterial that may provide an insight into the development of new drugs.
[Mh] Termos MeSH primário: Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Peptídeos Catiônicos Antimicrobianos/isolamento & purificação
Peptídeos Catiônicos Antimicrobianos/farmacologia
Bactérias/efeitos dos fármacos
Muco/química
Urodelos
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/toxicidade
Peptídeos Catiônicos Antimicrobianos/química
Peptídeos Catiônicos Antimicrobianos/toxicidade
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Dicroísmo Circular
Hepatócitos/efeitos dos fármacos
Seres Humanos
Programas de Rastreamento/métodos
Espectrometria de Massas
Peso Molecular
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antimicrobial Cationic Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE



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