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[PMID]:29413583
[Au] Autor:Cao X; Jiang Z; Wang S; Hong S; Li H; Zhang C; Shao Y; She Y; Jin F; Jin M; Wang J
[Ad] Endereço:Key Laboratory of Agri-food Safety and Quality, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Ministry of Agriculture of China, Beijing, 100081, PR China.
[Ti] Título:Metal-organic framework UiO-66 for rapid dispersive solid phase extraction of neonicotinoid insecticides in water samples.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1077-1078:92-97, 2018 Mar 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:UIO-66 crystals were explored for the first time to adsorb neonicotinoid insecticides in environmental water samples. HPLC coupled with tandem MS was used for quantification and determination of neonicotinoid insecticides. UiO-66 crystals was successfully synthesized by a simple constant-temperature bath method. Synthesized UiO-66 was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetry and nitrogen adsorption porosimetry (NAP), which demonstrated a uniform particle size, a large Brunauer-Emmett-Teller (BET) surface area and high thermostability. The adsorbing results showed that UIO-66 crystals could be used as a promising adsorbents for rapid extraction of neonicotinoid insecticides and be reused at least 10 times. Under the optimized conditions, the limits of detection (LODs, S/N = 3) and limits of quantification (LOQs, S/N = 10) for the five insecticides were found to be 0.02-0.4 ng/mL and 0.05-1.0 ng/mL, respectively. This developed approach not only provided more simple and sensitive method, as well as possessing satisfactory recovery for neonicotinoid insecticides, but also for other traces in environmental samples.
[Mh] Termos MeSH primário: Inseticidas/isolamento & purificação
Estruturas Metalorgânicas/química
Neonicotinoides/isolamento & purificação
Extração em Fase Sólida/métodos
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Adsorção
Água Potável/química
Concentração de Íons de Hidrogênio
Inseticidas/análise
Inseticidas/química
Lagos/química
Limite de Detecção
Modelos Lineares
Neonicotinoides/análise
Neonicotinoides/química
Concentração Osmolar
Reprodutibilidade dos Testes
Poluentes Químicos da Água/análise
Poluentes Químicos da Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water); 0 (Insecticides); 0 (Metal-Organic Frameworks); 0 (Neonicotinoids); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


  2 / 50032 MEDLINE  
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[PMID]:28985538
[Au] Autor:Chillè D; Foti C; Giuffrè O
[Ad] Endereço:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, 98166 Messina, Italy.
[Ti] Título:Thermodynamic parameters for the protonation and the interaction of arsenate with Mg , Ca and Sr : Application to natural waters.
[So] Source:Chemosphere;190:72-79, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thermodynamic parameters for the protonation of AsO and for the interaction with Mg , Ca and Sr were reported, comprehensive also of their dependence on ionic strength, considering the 0.1 ≤ I ≤ 1 M range and using NaCl as background salt. The same speciation models were obtained for Mg , Ca and Sr systems, with the formation of three different species: ML, MLH and MLH (L = AsO ). Mono- and di-protonated species were very weak, with formation constant values (log K) ranging from 1.45 to 3.23. In order to have a complete picture of thermodynamic properties of the systems under study and to fill the shortage of thermodynamic data on arsenate complex systems, the ligand protonation and metal complex enthalpies were also determined by calorimetric titrations, at t = 25 °C and in NaCl at I = 0.7 M (for H -AsO species also at I = 0.1 M). On the light of the proposed speciation models, examples of As(V) distribution in some natural waters are reported.
[Mh] Termos MeSH primário: Arseniatos/química
Concentração Osmolar
Termodinâmica
Água/química
[Mh] Termos MeSH secundário: Cálcio/química
Calorimetria
Ligantes
Magnésio/química
Cloreto de Sódio/química
Estrôncio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenates); 0 (Ligands); 059QF0KO0R (Water); 451W47IQ8X (Sodium Chloride); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium); YZS2RPE8LE (Strontium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


  3 / 50032 MEDLINE  
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[PMID]:27776915
[Au] Autor:Mendler M; Riedinger C; Schlotterer A; Volk N; Fleming T; Herzig S; Nawroth PP; Morcos M
[Ad] Endereço:Department of Medicine 1 and Clinical Chemistry, University of Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany. Electronic address: michael.mendler@med.uni-heidelberg.de.
[Ti] Título:Reduction in ins-7 gene expression in non-neuronal cells of high glucose exposed Caenorhabditis elegans protects from reactive metabolites, preserves neuronal structure and head motility, and prolongs lifespan.
[So] Source:J Diabetes Complications;31(2):304-310, 2017 Feb.
[Is] ISSN:1873-460X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Glucose derived metabolism generates reactive metabolites affecting the neuronal system and lifespan in C. elegans. Here, the role of the insulin homologue ins-7 and its downstream effectors in the generation of high glucose induced neuronal damage and shortening of lifespan was studied. RESULTS: In C. elegans high glucose conditions induced the expression of the insulin homologue ins-7. Abrogating ins-7 under high glucose conditions in non-neuronal cells decreased reactive oxygen species (ROS)-formation and accumulation of methylglyoxal derived advanced glycation endproducts (AGEs), prevented structural neuronal damage and normalised head motility and lifespan. The restoration of lifespan by decreased ins-7 expression was dependent on the concerted action of sod-3 and glod-4 coding for the homologues of iron-manganese superoxide dismutase and glyoxalase 1, respectively. CONCLUSIONS: Under high glucose conditions mitochondria-mediated oxidative stress and glycation are downstream targets of ins-7. This impairs the neuronal system and longevity via a non-neuronal/neuronal crosstalk by affecting sod-3 and glod-4, thus giving further insight into the pathophysiology of diabetic complications.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/antagonistas & inibidores
Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Glucose/envenenamento
Lactoilglutationa Liase/metabolismo
Estresse Oxidativo
Hormônios Peptídicos/antagonistas & inibidores
Superóxido Dismutase/metabolismo
[Mh] Termos MeSH secundário: Animais
Comportamento Animal
Caenorhabditis elegans/enzimologia
Caenorhabditis elegans/crescimento & desenvolvimento
Proteínas de Caenorhabditis elegans/agonistas
Proteínas de Caenorhabditis elegans/genética
Retroalimentação Fisiológica
Técnicas de Silenciamento de Genes
Técnicas de Inativação de Genes
Produtos Finais de Glicação Avançada/metabolismo
Lactoilglutationa Liase/antagonistas & inibidores
Lactoilglutationa Liase/genética
Longevidade
Mutação
Neuroproteção
Concentração Osmolar
Hormônios Peptídicos/agonistas
Hormônios Peptídicos/genética
Hormônios Peptídicos/metabolismo
Interferência de RNA
Espécies Reativas de Oxigênio/metabolismo
Superóxido Dismutase/antagonistas & inibidores
Superóxido Dismutase/genética
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Glycation End Products, Advanced); 0 (Ins-7 protein, C elegans); 0 (Peptide Hormones); 0 (Reactive Oxygen Species); EC 1.15.1.1 (Sod-3 protein, C elegans); EC 1.15.1.1 (Superoxide Dismutase); EC 4.4.1.5 (Lactoylglutathione Lyase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  4 / 50032 MEDLINE  
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[PMID]:29184921
[Au] Autor:Jaworek MW; Schuabb V; Winter R
[Ad] Endereço:Physical Chemistry I - Biophysical Chemistry, Faculty of Chemistry and Chemical Biology, Technical University Dortmund, Otto-Hahn-Strasse 4a, 44227 Dortmund, Germany. roland.winter@tu-dortmund.de.
[Ti] Título:The effects of glycine, TMAO and osmolyte mixtures on the pressure dependent enzymatic activity of α-chymotrypsin.
[So] Source:Phys Chem Chem Phys;20(3):1347-1354, 2018 Jan 17.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:High pressure is an important feature of certain natural environments, such as the deep sea where pressures up to about 1000 bar are encountered. Further, pressure effects on biosystems are of increasing interest for biotechnological applications, such as baroenzymology. We studied the effect of two different natural osmolyte mixtures, with major components being glycine and trimethylamine-N-oxide (TMAO), on the activity of α-chymotrypsin, using high-pressure stopped-flow methodology in combination with fast UV/Vis detection. We show that pressure is not only able to drastically enhance the catalytic activity and efficiency of the enzyme, but also that glycine has a significant and diverse effect on the enzymatic activity and volumetric properties of the reaction compared to TMAO. The results might not only help to understand the modulation of enzymatic reactions by natural osmolytes, but also elucidate ways to optimize enzymatic processes in biotechnological applications.
[Mh] Termos MeSH primário: Quimotripsina/metabolismo
Glicina/química
Metilaminas/química
[Mh] Termos MeSH secundário: Quimotripsina/química
Glicina/metabolismo
Hidrólise
Cinética
Metilaminas/metabolismo
Concentração Osmolar
Pressão
Especificidade por Substrato
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Methylamines); EC 3.4.21.1 (Chymotrypsin); EC 3.4.21.1 (alpha-chymotrypsin); FLD0K1SJ1A (trimethyloxamine); TE7660XO1C (Glycine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06042d


  5 / 50032 MEDLINE  
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[PMID]:28470534
[Au] Autor:Manganelli S; Benfenati E
[Ad] Endereço:Department of Environmental Health Sciences, Laboratory of Environmental Chemistry and Toxicology, IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri", Via La Masa 19, Milan, 20156, Italy. serena.manganelli@marionegri.it.
[Ti] Título:Nano-QSAR Model for Predicting Cell Viability of Human Embryonic Kidney Cells.
[So] Source:Methods Mol Biol;1601:275-290, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traditional Quantitative Structure-Activity Relationships (QSAR) models based on molecular descriptors as translators of chemical information show some drawbacks in predicting toxicity of nanomaterials due to their unique properties and to their nonhomogeneous structure.This chapter provides instructions on how to use CORAL, freely available software for building nano-QSAR models. CORAL makes use of descriptors based on "quasi-SMILES" representing physicochemical features and/or experimental conditions as an alternative to traditional SMILES encoding chemical structure to build up predictive nano-QSAR models for cytotoxicity.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Nanopartículas/química
Nanopartículas/toxicidade
[Mh] Termos MeSH secundário: Linhagem Celular
Células HEK293
Seres Humanos
Estrutura Molecular
Concentração Osmolar
Tamanho da Partícula
Relação Quantitativa Estrutura-Atividade
Dióxido de Silício/química
Dióxido de Silício/toxicidade
Software
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7631-86-9 (Silicon Dioxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_22


  6 / 50032 MEDLINE  
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[PMID]:28465210
[Au] Autor:Ritchie EK; Martin EB; Racher A; Jaques C
[Ad] Endereço:Biopharmaceutical Bioprocessing Technology Centre, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK; Unilever, 100VE, 100 Victoria Embankment, London, EC4Y 0DY, UK. Electronic address: elspeth.ritchie@gmail.com.
[Ti] Título:Extraction of indirectly captured information for use in a comparison of offline pH measurement technologies.
[So] Source:J Biotechnol;251:160-165, 2017 Jun 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Understanding the causes of discrepancies in pH readings of a sample can allow more robust pH control strategies to be implemented. It was found that 59.4% of differences between two offline pH measurement technologies for an historical dataset lay outside an expected instrument error range of ±0.02pH. A new variable, Osmo , was created using multiple linear regression (MLR) to extract information indirectly captured in the recorded measurements for osmolality. Principal component analysis and time series analysis were used to validate the expansion of the historical dataset with the new variable Osmo . MLR was used to identify variables strongly correlated (p<0.05) with differences in pH readings by the two offline pH measurement technologies. These included concentrations of specific chemicals (e.g. glucose) and Osmo indicating culture medium and bolus feed additions as possible causes of discrepancies between the offline pH measurement technologies. Temperature was also identified as statistically significant. It is suggested that this was a result of differences in pH-temperature compensations employed by the pH measurement technologies. In summary, a method for extracting indirectly captured information has been demonstrated, and it has been shown that competing pH measurement technologies were not necessarily interchangeable at the desired level of control (±0.02pH).
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/instrumentação
Concentração de Íons de Hidrogênio
[Mh] Termos MeSH secundário: Animais
Reatores Biológicos
Células CHO
Cricetulus
Modelos Lineares
Concentração Osmolar
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  7 / 50032 MEDLINE  
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[PMID]:27773805
[Au] Autor:Camilleri M; Sellin JH; Barrett KE
[Ad] Endereço:Clinical Enteric Neuroscience Translational and Epidemiological Research, Division of Gastroenterology and Hepatology, Department of Medicine, Mayo Clinic, Rochester, Minnesota. Electronic address: camilleri.michael@mayo.edu.
[Ti] Título:Pathophysiology, Evaluation, and Management of Chronic Watery Diarrhea.
[So] Source:Gastroenterology;152(3):515-532.e2, 2017 Feb.
[Is] ISSN:1528-0012
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic watery diarrhea poses a diagnostic and therapeutic challenge and is often a disabling condition for patients. Although acute diarrhea is likely to be caused by infection, the causes of chronic diarrhea (>4 weeks in duration) are more elusive. We review the pathophysiology, diagnosis, and treatment of chronic diarrhea. Drawing on recent insights into the molecular mechanisms of intestinal epithelial transport and barrier function, we discuss how diarrhea can result from a decrease in luminal solute absorption, an increase in secretion, or both, as well as derangements in barrier properties. We also describe the various extraepithelial factors that activate diarrheal mechanisms. Finally, clinical evaluation and tests used in the assessment of patients presenting with chronic diarrhea are reviewed, and an algorithm guiding therapeutic decisions and pharmacotherapy is presented.
[Mh] Termos MeSH primário: Diarreia/metabolismo
Absorção Intestinal
Secreções Intestinais
Intestinos/metabolismo
[Mh] Termos MeSH secundário: Proteína C-Reativa/metabolismo
Cromograninas/metabolismo
Doença Crônica
Diarreia/diagnóstico
Diarreia/fisiopatologia
Diarreia/terapia
Fezes/química
Motilidade Gastrointestinal
Seres Humanos
Inflamação
Intestinos/fisiopatologia
Síndrome do Intestino Irritável/metabolismo
Lactoferrina/metabolismo
Complexo Antígeno L1 Leucocitário/metabolismo
Concentração Osmolar
Permeabilidade
Prostaglandinas/metabolismo
Serotonina/metabolismo
Substância P/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chromogranins); 0 (Leukocyte L1 Antigen Complex); 0 (Prostaglandins); 333DO1RDJY (Serotonin); 33507-63-0 (Substance P); 9007-41-4 (C-Reactive Protein); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 50032 MEDLINE  
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[PMID]:29107877
[Au] Autor:Lin S; Yang H; Na Z; Lin K
[Ad] Endereço:State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: linsen@ecust.edu.cn.
[Ti] Título:A novel biodegradable arsenic adsorbent by immobilization of iron oxyhydroxide (FeOOH) on the root powder of long-root Eichhornia crassipes.
[So] Source:Chemosphere;192:258-266, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, FeOOH was immobilized on the biodegradable root powder, abbreviated as RP, of long-root Eichhornia crassipes, a kind of waste biomass, to improve the adsorption performances for aqueous arsenic contaminants. The adsorption kinetics and thermodynamics experiments showed that the adsorption rates and capacities of the root powder for arsenate (As(V)) and arsenite (As(III)) were both enhanced markedly after modification with FeOOH. The adsorption of As(V) and As(III) by the modified root powder, abbreviated as MRP, could arrive at equilibrium in 50 min and the saturated adsorption capacities reached up to 8.67-9.43 mg/g for As(V) and 5.21-5.65 mg/g for As(V) at temperature of 10-50 °C, respectively. Besides, the effect of pH and ionic strength on adsorption was investigated and the results showed that the optimum pH for the arsenic adsorption using the MRP was 9.0 and the As(V) adsorption was more sensitive to ionic strength. Furthermore, the complexation of hydratable hydroxyls on FeOOH with arsenic contaminants was concluded as the adsorption force according FTIR and XPS analyses. The MRP used could be regenerated via 0.4 mol/L NaOH solution and no apparent adsorption capacity losses appeared after 6 cyclic utilizations.
[Mh] Termos MeSH primário: Arsenicais/metabolismo
Eichhornia/metabolismo
Preparações de Plantas/metabolismo
Poluentes Químicos da Água/metabolismo
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Adsorção
Arseniatos/metabolismo
Arsênico/metabolismo
Arsenitos/metabolismo
Compostos Férricos/metabolismo
Concentração de Íons de Hidrogênio
Concentração Osmolar
Raízes de Plantas/metabolismo
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenates); 0 (Arsenicals); 0 (Arsenites); 0 (Ferric Compounds); 0 (Plant Preparations); 0 (Water Pollutants, Chemical); 2UA751211N (ferric hydroxide); N5509X556J (arsenite); N712M78A8G (Arsenic); N7CIZ75ZPN (arsenic acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171107
[St] Status:MEDLINE


  9 / 50032 MEDLINE  
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[PMID]:29211994
[Au] Autor:Wu XS; Elias S; Liu H; Heureaux J; Wen PJ; Liu AP; Kozlov MM; Wu LG
[Ad] Endereço:National Institute of Neurological Disorders and Stroke, Bethesda, Maryland.
[Ti] Título:Membrane Tension Inhibits Rapid and Slow Endocytosis in Secretory Cells.
[So] Source:Biophys J;113(11):2406-2414, 2017 Dec 05.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Endocytosis generates spherical or ellipsoid-like vesicles from the plasma membrane, which recycles vesicles that fuse with the plasma member during exocytosis in neurons and endocrine secretory cells. Although tension in the plasma membrane is generally considered to be an important factor in regulating endocytosis, whether membrane tension inhibits or facilitates endocytosis remains debated in the endocytosis field, and has been rarely studied for vesicular endocytosis in secretory cells. Here we report that increasing membrane tension by adjusting osmolarity inhibited both the rapid (a few seconds) and slow (tens of seconds) endocytosis in calyx-type nerve terminals containing conventional active zones and in neuroendocrine chromaffin cells. We address the mechanism of this phenomenon by computational modeling of the energy barrier that the system must overcome at the stage of membrane budding by an assembling protein coat. We show that this barrier grows with increasing tension, which may slow down or prevent membrane budding. These results suggest that in live secretory cells, membrane tension exerts inhibitory action on endocytosis.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Células Cromafins/citologia
Células Cromafins/metabolismo
Endocitose
[Mh] Termos MeSH secundário: Animais
Feminino
Espaço Intracelular/metabolismo
Cinética
Masculino
Camundongos
Concentração Osmolar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  10 / 50032 MEDLINE  
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[PMID]:29267370
[Au] Autor:Robergs RA
[Ad] Endereço:School of Exercise and Nutrition Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, Queensland, Australia.
[Ti] Título:Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle.
[So] Source:PLoS One;12(12):e0189822, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose), completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-'ve values = H+ release) for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate), and -1.96 and -0.01 (lactate), respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and -0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non-mitochondrial energy metabolism, and reveal the greater role of glycolysis in net H+ release than previously assumed. The data can also be used to improve the understanding of the cause of metabolic acidosis, and reveal mechanistic connections between H+ release within and from muscle and the electrochemical neutrality concepts that further refine acid-base balance in biological solutions.
[Mh] Termos MeSH primário: Cátions/metabolismo
Músculo Esquelético/metabolismo
Fosfatos/metabolismo
Prótons
[Mh] Termos MeSH secundário: Animais
Ligação Competitiva
Metabolismo Energético
Glicólise
Concentração de Íons de Hidrogênio
Modelos Teóricos
Concentração Osmolar
Temperatura Ambiente
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Phosphates); 0 (Protons)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189822



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