Base de dados : MEDLINE
Pesquisa : G02.700 [Categoria DeCS]
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  1 / 164609 MEDLINE  
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[PMID]:29431326
[Au] Autor:Khripach LV; Zheleznyak EV; Knyazeva TD; Koganova ZI; Salikhova DI; Grishin DA
[Ti] Título:[Method of colored model radicals for assessment of oxidative equilibrium in biologic samples].
[So] Source:Gig Sanit;95(9):884-90, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The most specific method of the recording of the rate offree radical reactions is the method of electron paramagnetic resonance (EPR) spectroscopy, but it is rarely used in applied biology due to expensive equipment and complexity of the execution of measurements. However chemists have found a number of colored organic radicals which lose the coloring under transition into diamagnetic form. In the given paper there are presented results of our studies on the development of methods for the assessment of oxidant equilibrium in biological media with a use of stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cation-radicals of N,N-diethyl-p-phenylenediamine (DEPPD). We have developed the new modification of DPPH test, replacing methanol-based incubation medium by non-ionic detergent solution, compatible with native blood serum. Modified DPPH test conserved typical biphasic kinetics of the origin variant, had the similar sensitivity to model antioxidants (IC values 49, 38 and 13 mkMfor ascorbate, a-tocopherol and quercetine, correspondingly) and was applied in experiments on laboratory animals treated with nano- and ionic silver, carbon nanotubes, microfine coal and electrolytic dust. We have tried also the assay of serum lipid hydroperoxides based on Fe-initiated DEPPD oxidation (Alberti et al., 2000). The comparison of kinetics of DEPPD oxidation in model (HO/Fe) and biologic (rat serum/Fe) systems, before and after Fe addition, seems to be an evidence that ceruloplasmin (CP) was involved in the resulting process, but failed to determine its polynomial kinetics, at least for the rat serum and DEPPD excess. The use of CP monoclonal antibodies seems to be the best way for the clarification of the mechanism of this reaction.
[Mh] Termos MeSH primário: Compostos de Bifenilo
Oxirredução
Fenilenodiaminas
Picratos
Plasma
[Mh] Termos MeSH secundário: Animais
Fenômenos Bioquímicos
Compostos de Bifenilo/análise
Compostos de Bifenilo/química
Compostos de Bifenilo/metabolismo
Corantes/análise
Corantes/química
Indicadores e Reagentes/análise
Indicadores e Reagentes/química
Modelos Químicos
Fenilenodiaminas/análise
Fenilenodiaminas/química
Fenilenodiaminas/metabolismo
Picratos/análise
Picratos/química
Picratos/metabolismo
Plasma/química
Plasma/metabolismo
Ratos
Kit de Reagentes para Diagnóstico
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Coloring Agents); 0 (Indicators and Reagents); 0 (Phenylenediamines); 0 (Picrates); 0 (Reagent Kits, Diagnostic); 93-05-0 (N,N-diethyl 4-phenylenediamine); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  2 / 164609 MEDLINE  
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[PMID]:29367490
[Au] Autor:Asakawa T; Takano Y; Ohta A; Asakawa H
[Ad] Endereço:School of Chemistry, College of Science and Engineering, Kanazawa University.
[Ti] Título:Aggregation and pH Responsive Behavior of Thioester Surfactants and Formation of Disulfide Linkages in Aqueous Solutions.
[So] Source:J Oleo Sci;67(2):199-206, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:pH responsive surfactants, [C H N(CH ) (CH ) SCOCH ]Br (C nSAc, n = 4, 11, 12), were prepared, and their properties in aqueous solution were examined. The critical micelle concentration (cmc) and critical vesicle concentration (cvc) were determined based on changes in conductivity, as well as by fluorescence measurements, and light scattering methods. A significant increase in the light scattering intensities of the C nSAc (n=11, 12) systems suggested that the growth of aggregates was accompanied by considerable counterion binding with increasing surfactant concentration. The diameter of C 11SAc, recorded by the dynamic light scattering measurements, was about 9.6 ±1.0 nm, which was slightly smaller than that for didodecyldimethylammonium bromide (DDAB) vesicles. The thioester group was easily hydrolyzed upon the addition of NaOH, while it was hardly hydrolyzed with the addition of HCl. The time course of alkaline hydrolysis was examined by the conductivity measurements and high-performance liquid chromatography analysis. [C H N(CH ) (CH ) SS(CH ) N(CH ) C H ]2Br (2C 11SS) was generated in the C 11SAc alkaline solution because of air oxidation. The C 11SAc alkaline solution gradually became an opaque blue color with increasing light scattering at 346 nm, indicating the remarkable growth of vesicles. The chemical structure of 2C 11SS was consistent with that of a disulfide linked double tailed surfactant, similar to DDAB. The disulfide linkage between the double tailed surfactants will contribute to the stabilization and growth of vesicles.
[Mh] Termos MeSH primário: Ésteres/química
Tensoativos/química
Água/química
[Mh] Termos MeSH secundário: Dissulfetos/química
Difusão Dinâmica da Luz
Concentração de Íons de Hidrogênio
Hidrólise
Micelas
Oxirredução
Compostos de Amônio Quaternário/química
Soluções
Tensão Superficial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Esters); 0 (Micelles); 0 (Quaternary Ammonium Compounds); 0 (Solutions); 0 (Surface-Active Agents); 059QF0KO0R (Water); 13146-86-6 (didodecyldimethylammonium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17192


  3 / 164609 MEDLINE  
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[PMID]:29353041
[Au] Autor:Hayashi Y; Shimamura A; Ishikawa T; Fujiwara Y; Ichi I
[Ad] Endereço:Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo, Japan.
[Ti] Título:FADS2 inhibition in essential fatty acid deficiency induces hepatic lipid accumulation via impairment of very low-density lipoprotein (VLDL) secretion.
[So] Source:Biochem Biophys Res Commun;496(2):549-555, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD). In this study, an obvious hepatic lipid accumulation was observed in EFAD mice treated with a FADS2 inhibitor. FADS2 inhibition in the EFAD state reduced secretion of very low-density lipoprotein (VLDL) and markedly diminished Mead acid in phosphatidylcholine (PC) in the liver and plasma. As the results, the amount of C20 HUFAs in hepatic and plasma PC dramatically reduced in the EFAD mice treated with a FADS2 inhibitor, whereas the decrease of C20 HUFA levels of PC in EFAD mice was not observed because of the increased Mead acid in PC. These results supposed that Mead acid in PC is important as a component of VLDL. It is possible that Mead acid plays the role of a substitute of HUFAs in VLDL secretion during EFAD.
[Mh] Termos MeSH primário: Ácidos Graxos Dessaturases/metabolismo
Ácidos Graxos Insaturados/metabolismo
Lipoproteínas VLDL/metabolismo
Fígado/metabolismo
[Mh] Termos MeSH secundário: Ácido 8,11,14-Eicosatrienoico/análogos & derivados
Ácido 8,11,14-Eicosatrienoico/metabolismo
Animais
Ácidos Graxos Dessaturases/antagonistas & inibidores
Fígado Gorduroso/metabolismo
Metabolismo dos Lipídeos
Masculino
Camundongos Endogâmicos C57BL
Oxirredução
Fosfatidilcolinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids, Unsaturated); 0 (Lipoproteins, VLDL); 0 (Phosphatidylcholines); 7324-41-6 (8,11,14-Eicosatrienoic Acid); EC 1.14.19.- (Fatty Acid Desaturases); EC 1.14.19.3 (FADS2 protein, mouse); JQS194YH3X (mead acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180122
[St] Status:MEDLINE


  4 / 164609 MEDLINE  
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[PMID]:29214538
[Au] Autor:Akbarzade S; Chamsaz M; Rounaghi GH; Ghorbani M
[Ad] Endereço:Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
[Ti] Título:Zero valent Fe-reduced graphene oxide quantum dots as a novel magnetic dispersive solid phase microextraction sorbent for extraction of organophosphorus pesticides in real water and fruit juice samples prior to analysis by gas chromatography-mass spectrometry.
[So] Source:Anal Bioanal Chem;410(2):429-439, 2018 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A selective and sensitive magnetic dispersive solid-phase microextraction (MDSPME) coupled with gas chromatography-mass spectrometry was developed for extraction and determination of organophosphorus pesticides (Sevin, Fenitrothion, Malathion, Parathion, and Diazinon) in fruit juice and real water samples. Zero valent Fe-reduced graphene oxide quantum dots (rGOQDs@ Fe) as a new and effective sorbent were prepared and applied for extraction of organophosphorus pesticides using MDSPME method. In order to study the performance of this new sorbent, the ability of rGOQDs@ Fe was compared with graphene oxide and magnetic graphene oxide nanocomposite by recovery experiments of the organophosphorus pesticides. Several affecting parameters in the microextraction procedure, including pH of donor phase, donor phase volume, stirring rate, extraction time, and desorption conditions such as the type and volume of solvents and desorption time were thoroughly investigated and optimized. Under the optimal conditions, the method showed a wide linear dynamic range with R-square between 0.9959 and 0.9991. The limit of detections, the intraday and interday relative standard deviations (n = 5) were less than 0.07 ngmL , 4.7, and 8.6%, respectively. The method was successfully applied for extraction and determination of organophosphorus pesticides in real water samples (well, river and tap water) and fruit juice samples (apple and grape juice). The obtained relative recoveries were in the range of 82.9%-113.2% with RSD percentages of less than 5.8% for all the real samples.
[Mh] Termos MeSH primário: Sucos de Frutas e Vegetais/análise
Grafite/química
Compostos Organofosforados/análise
Praguicidas/análise
Microextração em Fase Sólida/métodos
Poluentes Químicos da Água/análise
Água/análise
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas/métodos
Ferro/química
Limite de Detecção
Compostos Organofosforados/isolamento & purificação
Oxirredução
Óxidos/química
Praguicidas/isolamento & purificação
Pontos Quânticos/química
Poluentes Químicos da Água/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Organophosphorus Compounds); 0 (Oxides); 0 (Pesticides); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 7782-42-5 (Graphite); E1UOL152H7 (Iron)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-017-0732-9


  5 / 164609 MEDLINE  
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[PMID]:29184993
[Au] Autor:Khoury S; Pouyet C; Lyan B; Pujos-Guillot E
[Ad] Endereço:Université Clermont Auvergne, INRA, UNH, Plateforme d'Exploration du Métabolisme, MetaboHUB Clermont, CRNH Auvergne, F-63000, Clermont-Ferrand, France. spiro.khoury@inra.fr.
[Ti] Título:Evaluation of oxidized phospholipids analysis by LC-MS/MS.
[So] Source:Anal Bioanal Chem;410(2):633-647, 2018 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Phospholipids (PLs) represent a class of metabolites of interest for evaluating the relationship between diet and the development of several metabolic diseases. Given that PLs are rich in unsaturated fatty acids, they can be oxidized. Because of their structure and reactivity, oxidized phospholipids (PLs-Ox) are increasingly recognized as markers of oxidative stress and of various diseases associated with inflammation. Therefore, there is a growing interest in studying PLs-Ox in lipidomics. Because of their limited commercial availability, very little information is currently available in databases to identify these molecules. The aim of this study is to acquire new knowledge about PLs-Ox in order to propose an analytical strategy for their analyses. For this purpose, a synthesis method of PLs-Ox, in auto-oxidation, has been developed and applied on phosphatidylcholine and phosphatidylethanolamine molecular species with various chain lengths, degree, and position of unsaturations. An analysis method based on mass (MS) and tandem mass spectrometry coupled to electrospray ionization was then developed and enabled the identification of a great diversity of long- and short-chain oxidation products. Formation kinetics of oxidation products was evaluated. Results showed that the formation of oxidized compounds was largely influenced by the degree of unsaturation on fatty acid chains. Oxidation time promotes the formation of some biologically important oxidation products. Coupling the MS method with liquid chromatography in flow injection analysis mode enabled the development of a full analytical strategy. Structural analysis of PLs-Ox allowed the enrichment of databases with important information to identify these molecules in biological matrices.
[Mh] Termos MeSH primário: Fosfolipídeos/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Oxirredução
Estresse Oxidativo
Espectrometria de Massas por Ionização por Electrospray/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phospholipids)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-017-0764-1


  6 / 164609 MEDLINE  
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[PMID]:29175401
[Au] Autor:Wan J; Du X; Wang R; Liu E; Jia J; Bai X; Hu X; Fan J
[Ad] Endereço:School of Chemical Engineering, Northwest University, Xi'an 710069, PR China.
[Ti] Título:Mesoporous nanoplate multi-directional assembled Bi WO for high efficient photocatalytic oxidation of NO.
[So] Source:Chemosphere;193:737-744, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herein, a mesoporous nanoplate multi-directional assembled Bi WO architecture was successfully prepared and applied for the photocatalytic removal of NOx pollutants at low concentrations under visible light and simulated solar light irradiation. Bi WO -180-C synthesized at a hydrothermal temperature of 180 °C with calcination exhibited an excellent conversion efficiency in the photocatalytic oxidation of gaseous NO. The crystallinity, morphology, specific surface area, pore environment, light absorption, and separation of photogenerated electrons and holes were investigated by various techniques; the excellent photocatalytic performance of Bi WO -180-C was attributed to its special hierarchical mesoporous structure with an appropriate pore size and interconnected porous network, which imparted good gas permeability and fast mass transfer of reaction intermediates and final products of NO oxidation. Furthermore, hierarchical mesoporous Bi WO showed excellent photocatalytic durability and reusability.
[Mh] Termos MeSH primário: Bismuto/química
Catálise
Óxido Nítrico/química
Óxido Nítrico/efeitos da radiação
[Mh] Termos MeSH secundário: Gases
Luz
Oxidantes Fotoquímicos
Oxirredução
Porosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gases); 0 (Oxidants, Photochemical); 31C4KY9ESH (Nitric Oxide); U015TT5I8H (Bismuth)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  7 / 164609 MEDLINE  
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[PMID]:29175398
[Au] Autor:Qiu N; Wang R; Sun Y; Wang X; Jiang D; Meng Y; Zhou F
[Ad] Endereço:School of Life Science, Qufu Normal University, Qufu, Shandong 273165, China.
[Ti] Título:Toxic effects and mechanism of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) on Lemna minor.
[So] Source:Chemosphere;193:711-719, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To investigate the toxic effect and mechanism of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in aquatic plants, in vivo and in vitro exposure to BDE-47 were conducted. After 14-d exposure to 5-20 µg/L BDE-47, the growth of Lemna minor plants was significantly suppressed, and the chlorophyll and soluble protein contents in fronds markedly decreased. Accordingly, the photosynthetic efficiency (Fv/Fm, PI) decreased. When the thylakoid membranes isolated from healthy fronds was exposed to 5-20 mg/L BDE-47 directly in vitro for 1 h, the photosynthetic efficiency also decreased significantly. In both the in vitro (5-20 µg/L) and in vivo (5-20 mg/L) experiments, BDE-47 led to an increased plasma membrane permeability. Hence, we concluded that BDE-47 had a direct toxicity to photosynthetic membranes and plasma membranes. However, direct effects on the activities of peroxidase (POD), malate dehydrogenase (MDH) and nitroreductase (NR) were not observed by adding 5-20 mg/L BDE-47 into crude enzyme extracts. The malondialdehyde (MDA) and superoxide anion radical (O ) contents in the BDE-47 treated fronds were higher than those in the control fronds, suggesting that L. minor can not effectively relieve reactive oxygen species (ROS). The data above indicates that BDE-47 is toxic to L. minor through acting directly on biomembranes, which induces the production of ROS and thus causes remarkable oxidative damage to cells.
[Mh] Termos MeSH primário: Araceae/efeitos dos fármacos
Éteres Difenil Halogenados/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Araceae/enzimologia
Araceae/metabolismo
Clorofila/metabolismo
Éter
Malondialdeído/metabolismo
Oxirredução
Peroxidase/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Halogenated Diphenyl Ethers); 0 (Reactive Oxygen Species); 0 (Water Pollutants, Chemical); 0F5N573A2Y (Ether); 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether); 1406-65-1 (Chlorophyll); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  8 / 164609 MEDLINE  
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[PMID]:28986298
[Au] Autor:Hsiao JC; McGrath AP; Kielmann L; Kalimuthu P; Darain F; Bernhardt PV; Harmer J; Lee M; Meyers K; Maher MJ; Kappler U
[Ad] Endereço:Centre for Metals in Biology, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
[Ti] Título:The central active site arginine in sulfite oxidizing enzymes alters kinetic properties by controlling electron transfer and redox interactions.
[So] Source:Biochim Biophys Acta;1859(1):19-27, 2018 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A central conserved arginine, first identified as a clinical mutation leading to sulfite oxidase deficiency, is essential for catalytic competency of sulfite oxidizing molybdoenzymes, but the molecular basis for its effects on turnover and substrate affinity have not been fully elucidated. We have used a bacterial sulfite dehydrogenase, SorT, which lacks an internal heme group, but transfers electrons to an external, electron accepting cytochrome, SorU, to investigate the molecular functions of this arginine residue (Arg78). Assay of the SorT Mo centre catalytic competency in the absence of SorU showed that substitutions in the central arginine (R78Q, R78K and R78M mutations) only moderately altered SorT catalytic properties, except for R78M which caused significant reduction in SorT activity. The substitutions also altered the Mo-centre redox potentials (Mo potential lowered by ca. 60-80mV). However, all Arg78 mutations significantly impaired the ability of SorT to transfer electrons to SorU, where activities were reduced 17 to 46-fold compared to SorT , precluding determination of kinetic parameters. This was accompanied by the observation of conformational changes in both the introduced Gln and Lys residues in the crystal structure of the enzymes. Taking into account data collected by others on related SOE mutations we propose that the formation and maintenance of an electron transfer complex between the Mo centre and electron accepting heme groups is the main function of the central arginine, and that the reduced turnover and increases in K are caused by the inefficient operation of the oxidative half reaction of the catalytic cycle in enzymes carrying these mutations.
[Mh] Termos MeSH primário: Arginina/química
Proteínas de Bactérias/química
Sinorhizobium meliloti/enzimologia
Sulfito Desidrogenase/química
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Arginina/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Domínio Catalítico
Transporte de Elétrons
Cinética
Molibdênio
Mutação de Sentido Incorreto
Oxirredução
Sinorhizobium meliloti/genética
Sulfito Desidrogenase/genética
Sulfito Desidrogenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 81AH48963U (Molybdenum); 94ZLA3W45F (Arginine); EC 1.8.2.1 (Sulfite Dehydrogenase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171008
[St] Status:MEDLINE


  9 / 164609 MEDLINE  
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[PMID]:28841524
[Au] Autor:Bharagava RN; Mishra S
[Ad] Endereço:Laboratory for Bioremediation and Metagenomics Research (LBMR), Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow 226025, U.P., India. Electronic address: bharagavarnbbau11@gmail.com.
[Ti] Título:Hexavalent chromium reduction potential of Cellulosimicrobium sp. isolated from common effluent treatment plant of tannery industries.
[So] Source:Ecotoxicol Environ Saf;147:102-109, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Present study deals with the isolation and characterization of a bacterium capable for the effective reduction of Cr(VI) from tannery wastewater. Based on the 16S rRNA gene sequence analysis, this bacterium was identified as Cellulosimicrobium sp. (KX710177). During the Cr(VI) reduction experiment performed at 50, 100, 200,and 300mg/L of Cr(VI) concentrations, the bacterium showed 99.33% and 96.98% reduction at 50 and 100mg/L at 24 and 96h, respectively. However, at 200 and 300mg/L concentration of Cr(VI), only 84.62% and 62.28% reduction was achieved after 96h, respectively. The SEM analysis revealed that bacterial cells exposed to Cr(VI) showed increased cell size in comparison to unexposed cells, which might be due to either the precipitation or adsorption of reduced Cr(III) on bacterial cells. Further, the Energy Dispersive X-ray (EDX) analysis showed some chromium peaks for cells exposed to Cr(VI), which might be either due to the presence of precipitated reduced Cr(III) on cells or complexation of Cr(III) with cell surface molecules. The bacterium also showed resistance and sensitivity against the tested antibiotics with a wide range of MIC values ranging from 250 to 800mg/L for different heavy metals. Thus, this multi-drug and multi-metal resistant bacterium can be used as a potential agent for the effective bioremediation of metal contaminated sites.
[Mh] Termos MeSH primário: Cromo/análise
Micrococcaceae/isolamento & purificação
Curtume
Águas Residuais/microbiologia
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cromo/química
Cromo/toxicidade
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Micrococcaceae/efeitos dos fármacos
Micrococcaceae/genética
Oxirredução
RNA Ribossômico 16S/genética
Águas Residuais/análise
Poluentes Químicos da Água/química
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Waste Water); 0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium); 18540-29-9 (chromium hexavalent ion)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


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[PMID]:28462587
[Au] Autor:Whon TW; Shin NR; Jung MJ; Hyun DW; Kim HS; Kim PS; Bae JW
[Ad] Endereço:Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University , Seoul, Republic of Korea.
[Ti] Título:Conditionally Pathogenic Gut Microbes Promote Larval Growth by Increasing Redox-Dependent Fat Storage in High-Sugar Diet-Fed Drosophila.
[So] Source:Antioxid Redox Signal;27(16):1361-1380, 2017 Dec 01.
[Is] ISSN:1557-7716
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIMS: Changes in the composition of the gut microbiota contribute to the development of obesity and subsequent complications that are associated with metabolic syndrome. However, the role of increased numbers of certain bacterial species during the progress of obesity and factor(s) controlling the community structure of gut microbiota remain unclear. Here, we demonstrate the inter-relationship between Drosophila melanogaster and their resident gut microbiota under chronic high-sugar diet (HSD) conditions. RESULTS: Chronic feeding of an HSD to Drosophila resulted in a predominance of resident uracil-secreting bacteria in the gut. Axenic insects mono-associated with uracil-secreting bacteria or supplemented with uracil under HSD conditions promoted larval development. Redox signaling induced by bacterial uracil promoted larval growth by regulating sugar and lipid metabolism via activation of p38a mitogen-activated protein kinase. INNOVATION: The present study identified a new redox-dependent mechanism by which uracil-secreting bacteria (previously regarded as opportunistic pathobionts) protect the host from metabolic perturbation under chronic HSD conditions. CONCLUSION: These results illustrate how Drosophila and gut microbes form a symbiotic relationship under stress conditions, and changes in the gut microbiota play an important role in alleviating deleterious diet-derived effects such as hyperglycemia. Antioxid. Redox Signal. 27, 1361-1380.
[Mh] Termos MeSH primário: Drosophila melanogaster/crescimento & desenvolvimento
Vida Livre de Germes
Obesidade/microbiologia
Sacarose/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Bactérias/classificação
Bactérias/isolamento & purificação
Bactérias/metabolismo
Modelos Animais de Doenças
Drosophila melanogaster/microbiologia
Trato Gastrointestinal/microbiologia
Metabolismo dos Lipídeos
Microbiota
Proteína Quinase 14 Ativada por Mitógeno/metabolismo
Obesidade/induzido quimicamente
Oxirredução
Uracila/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
56HH86ZVCT (Uracil); 57-50-1 (Sucrose); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 14)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1089/ars.2016.6790



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