Base de dados : MEDLINE
Pesquisa : G02.860.327 [Categoria DeCS]
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[PMID]:27839679
[Au] Autor:Hamano H; Nakamura S; Hayakawa J; Miyashita H; Harayama S
[Ad] Endereço:Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan. Electronic address: harayama_lab02@bio.chuo-u.ac.jp.
[Ti] Título:Biofilm-based photobioreactor absorbing water and nutrients by capillary action.
[So] Source:Bioresour Technol;223:307-311, 2017 Jan.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cells of the unicellular green alga, "Pseudochoricystis ellipsoidea", were uniformly spread on a cellulosic sheet or on a polytetrafluoroethylene (PTFE) membrane sheet superimposed on a cellulosic sheet at a density of 3.5-5.0gdry weight per m , and the sheet was adhered to an inverted V-shaped acrylic plate of 10cm in height. Several acrylic plates were placed side by side on a tray containing liquid medium at a depth of 0.6cm, and illuminated from above with a light intensity of 300-340µmolm s . Water and nutrients were supplied to cells by capillary action through the cellulosic sheet. Footprint biomass productivities of cells grown in atmospheric CO on this photobioreactor were 8-10gm day . This cultivation system is strongly energy- and labor-saving as it does not require mixing of culture fluid, irrigation of medium, and delivery of CO -enriched air.
[Mh] Termos MeSH primário: Técnicas de Cultura Celular por Lotes/métodos
Biofilmes
Ação Capilar
Alimentos
Fotobiorreatores/microbiologia
Água/metabolismo
[Mh] Termos MeSH secundário: Absorção Fisico-Química
Biofilmes/crescimento & desenvolvimento
Biomassa
Clorófitas/crescimento & desenvolvimento
Clorófitas/metabolismo
Luz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


  2 / 773 MEDLINE  
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[PMID]:27544807
[Au] Autor:Rustom LE; Boudou T; Lou S; Pignot-Paintrand I; Nemke BW; Lu Y; Markel MD; Picart C; Wagoner Johnson AJ
[Ad] Endereço:Department of Bioengineering, University of Illinois at Urbana-Champaign, 1270 Digital Computer Laboratory, MC-278, 1304 West Springfield Avenue, Urbana, IL 61801, USA; University Grenoble Alpes, LMGP, 38000 Grenoble, France. Electronic address: rustom2@illinois.edu.
[Ti] Título:Micropore-induced capillarity enhances bone distribution in vivo in biphasic calcium phosphate scaffolds.
[So] Source:Acta Biomater;44:144-54, 2016 10 15.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The increasing demand for bone repair solutions calls for the development of efficacious bone scaffolds. Biphasic calcium phosphate (BCP) scaffolds with both macropores and micropores (MP) have improved healing compared to those with macropores and no micropores (NMP), but the role of micropores is unclear. Here, we evaluate capillarity induced by micropores as a mechanism that can affect bone growth in vivo. Three groups of cylindrical scaffolds were implanted in pig mandibles for three weeks: MP were implanted either dry (MP-Dry), or after submersion in phosphate buffered saline, which fills pores with fluid and therefore suppresses micropore-induced capillarity (MP-Wet); NMP were implanted dry. The amount and distribution of bone in the scaffolds were quantified using micro-computed tomography. MP-Dry had a more homogeneous bone distribution than MP-Wet, although the average bone volume fraction, BVF‾, was not significantly different for these two groups (0.45±0.03 and 0.37±0.03, respectively). There was no significant difference in the radial bone distribution of NMP and MP-Wet, but the BVF‾, of NMP was significantly lower among the three groups (0.25±0.02). These results suggest that micropore-induced capillarity enhances bone regeneration by improving the homogeneity of bone distribution in BCP scaffolds. The explicit design and use of capillarity in bone scaffolds may lead to more effective treatments of large and complex bone defects. STATEMENT OF SIGNIFICANCE: The increasing demand for bone repair calls for more efficacious bone scaffolds and calcium phosphate-based materials are considered suitable for this application. Macropores (>100µm) are necessary for bone ingrowth and vascularization. However, studies have shown that microporosity (<20µm) also enhances growth, but there is no consensus on the controlling mechanisms. In previous in vitro work, we suggested that micropore-induced capillarity had the potential to enhance bone growth in vivo. This work illustrates the positive effects of capillarity on bone regeneration in vivo; it demonstrates that micropore-induced capillarity significantly enhances the bone distribution in the scaffold. The results will impact the design of scaffolds to better exploit capillarity and improve treatments for large and load-bearing bone defects.
[Mh] Termos MeSH primário: Osso e Ossos/efeitos dos fármacos
Fosfatos de Cálcio/farmacologia
Ação Capilar
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Regeneração Óssea/efeitos dos fármacos
Osso e Ossos/irrigação sanguínea
Osso e Ossos/diagnóstico por imagem
Tamanho do Órgão
Porosidade
Sus scrofa
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Calcium Phosphates); 97Z1WI3NDX (calcium phosphate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160822
[St] Status:MEDLINE


  3 / 773 MEDLINE  
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[PMID]:27455019
[Au] Autor:He T; Zhao H; Zhao X; Lu J; Zheng Y; Zhang C; Yin A
[Ad] Endereço:Medical Genetics Center, Key Laboratory of Metabolic and Genetic Disease in Women and Children, Reproductive Center, Guangdong Women and Children' Hospital, Guangzhou, Guangdong 511442, China. Email: yinaiwa@vip.126.com.
[Ti] Título:[Establishment of a screening method for AZF microdeletions by capillary technology and a clinical trial].
[So] Source:Zhonghua Yi Xue Yi Chuan Xue Za Zhi;33(4):550-4, 2016 Aug.
[Is] ISSN:1003-9406
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To establish an accurate, fast and simple screening method for AZF microdeletions using capillary technology and use it for clinical testing. METHODS: For each pair of primers, the 5' end of either forward or reverse primer was labeled with a FAM, JOE or TAMRA fluorescence dyes to establish multiplex quantitative fluorescence PCR systems for the establishment of a screening method of Y chromosome AZF microdeletions by capillary technology. The detection of Y chromosome AZF microdeletion was carried out on 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia. RESULTS: A screening method for Y chromosome AZF microdeletions using capillary technology was established. Thirty eight cases of AZF microdeletions were found among 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia, which gave a deletion rate of 5.24%. Y chromosomal microdeletions were found in 8.62% of the azoospermia group, 6.75% of the oligozoospermic group, and 2.23% of the asthenospermia group. CONCLUSION: An accurate, fast and simple screening method of Y chromosome AZF microdeletions by capillary technology has been established, which may have an important clinical value.
[Mh] Termos MeSH primário: Azoospermia/genética
Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Ação Capilar
Deleção Cromossômica
Cromossomos Humanos Y
Seres Humanos
Infertilidade Masculina
Masculino
Reação em Cadeia da Polimerase Multiplex
Aberrações dos Cromossomos Sexuais
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1003-9406.2016.04.028


  4 / 773 MEDLINE  
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[PMID]:27365041
[Au] Autor:Koczula KM; Gallotta A
[Ad] Endereço:Xeptagen SpA, VEGA Science Park, Via delle Industrie 9, Venice, Italy.
[Ti] Título:Lateral flow assays.
[So] Source:Essays Biochem;60(1):111-20, 2016 Jun 30.
[Is] ISSN:1744-1358
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients. The range and interpretation of results and parameters used for evaluation of the assay will also be discussed. The main advantages and disadvantages of LFAs will be summarized and relevant future improvements to testing devices and strategies will be proposed. Finally, the major recent advances and future diagnostic applications in the LFA field will be explored.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Ação Capilar
Técnicas de Diagnóstico Molecular/métodos
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/instrumentação
Imunoensaio/métodos
Membranas Artificiais
Técnicas de Diagnóstico Molecular/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Membranes, Artificial)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE
[do] DOI:10.1042/EBC20150012


  5 / 773 MEDLINE  
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[PMID]:27051882
[Au] Autor:Ni S; Leemann J; Buttinoni I; Isa L; Wolf H
[Ad] Endereço:Laboratory for Interfaces, Soft Matter, and Assembly, Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 5, 8093 Zurich, Switzerland.; IBM Research-Zurich, Säumerstrasse 4, 8803 Rüschlikon, Switzerland.
[Ti] Título:Programmable colloidal molecules from sequential capillarity-assisted particle assembly.
[So] Source:Sci Adv;2(4):e1501779, 2016 Apr.
[Is] ISSN:2375-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The assembly of artificial nanostructured and microstructured materials which display structures and functionalities that mimic nature's complexity requires building blocks with specific and directional interactions, analogous to those displayed at the molecular level. Despite remarkable progress in synthesizing "patchy" particles encoding anisotropic interactions, most current methods are restricted to integrating up to two compositional patches on a single "molecule" and to objects with simple shapes. Currently, decoupling functionality and shape to achieve full compositional and geometrical programmability remains an elusive task. We use sequential capillarity-assisted particle assembly which uniquely fulfills the demands described above. This is a new method based on simple, yet essential, adaptations to the well-known capillary assembly of particles over topographical templates. Tuning the depth of the assembly sites (traps) and the surface tension of moving droplets of colloidal suspensions enables controlled stepwise filling of traps to "synthesize" colloidal molecules. After deposition and mechanical linkage, the colloidal molecules can be dispersed in a solvent. The template's shape solely controls the molecule's geometry, whereas the filling sequence independently determines its composition. No specific surface chemistry is required, and multifunctional molecules with organic and inorganic moieties can be fabricated. We demonstrate the "synthesis" of a library of structures, ranging from dumbbells and triangles to units resembling bar codes, block copolymers, surfactants, and three-dimensional chiral objects. The full programmability of our approach opens up new directions not only for assembling and studying complex materials with single-particle-level control but also for fabricating new microscale devices for sensing, patterning, and delivery applications.
[Mh] Termos MeSH primário: Coloides/química
Nanotecnologia
Polímeros/química
[Mh] Termos MeSH secundário: Anisotropia
Ação Capilar
Nanoestruturas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Colloids); 0 (Polymers)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160407
[St] Status:MEDLINE
[do] DOI:10.1126/sciadv.1501779


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[PMID]:27021631
[Au] Autor:Ulum MF; Maylina L; Noviana D; Wicaksono DH
[Ad] Endereço:Medical Devices and Technology Group (MediTeg), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), Skudai 81310, Johor, Malaysia. dedy.wicaksono@gmail.com dedy@utm.my and Faculty of Veterinary Medicine, Bogor Agricultural University (IPB), Bogor, Indonesia. deni@ipb
[Ti] Título:EDTA-treated cotton-thread microfluidic device used for one-step whole blood plasma separation and assay.
[So] Source:Lab Chip;16(8):1492-504, 2016 Apr 21.
[Is] ISSN:1473-0189
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aims to observe the wicking and separation characteristics of blood plasma in a cotton thread matrix functioning as a microfluidic thread-based analytical device (µTAD). We investigated several cotton thread treatment methods using ethylenediaminetetraacetic acid (EDTA) anticoagulant solution for wicking whole blood samples and separating its plasma. The blood of healthy Indonesian thin tailed sheep was used in this study to understand the properties of horizontal wicking and separation on the EDTA-treated µTAD. The wicking distance and blood cell separation from its plasma was observed for 120 s and documented using a digital phone camera. The results show that untreated cotton-threads stopped the blood wicking process on the µTAD. On the other hand, the deposition of EDTA anticoagulant followed by its drying on the thread at room temperature for 10 s provides the longest blood wicking with gradual blood plasma separation. Furthermore, the best results in terms of the longest wicking and the clearest on-thread separation boundary between blood cells and its plasma were obtained using the µTAD treated with EDTA deposition followed by 60 min drying at refrigerated temperature (2-8 °C). The separation length of blood plasma in the µTADs treated with dried-EDTA at both room and refrigerated temperatures was not statistically different (P > 0.05). This separation occurs through the synergy of three factors, cotton fiber, EDTA anticoagulant and blood platelets, which induce the formation of a fibrin-filter via a partial coagulation process in the EDTA-treated µTAD. An albumin assay was employed to demonstrate the efficiency of this plasma separation method during a one-step assay on the µTAD. Albumin in blood is an important biomarker for kidney and heart disease. The µTAD has a slightly better limit of detection (LOD) than conventional blood analysis, with an LOD of 114 mg L(-1) compared to 133 mg L(-1), respectively. However, the µTAD performed faster to get results after 3 min compared to 14 min for centrifuged analysis of sheep blood samples. In conclusion, on-thread dried-EDTA anticoagulant deposition was able to increase the wicking distance and has a better capability to separate blood plasma and is suitable for combining separation and the assay system in a single device.
[Mh] Termos MeSH primário: Anticoagulantes/química
Separação Celular/instrumentação
Fibra de Algodão
Ácido Edético/química
Dispositivos Lab-On-A-Chip
Plasma/citologia
[Mh] Termos MeSH secundário: Animais
Anticoagulantes/farmacologia
Ação Capilar
Centrifugação
Ácido Edético/farmacologia
Plasma/efeitos dos fármacos
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anticoagulants); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161231
[Lr] Data última revisão:
161231
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.1039/c6lc00175k


  7 / 773 MEDLINE  
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[PMID]:26968140
[Au] Autor:Teng Y; Liu Y; Jiang L; Song Y; Zhao J; Zhang Y; Wang D
[Ad] Endereço:Key laboratory of ocean energy utilization and energy conservation of ministry of education, Dalian university of technology, Dalian 116024, China.
[Ti] Título:A visualization study on two-phase gravity drainage in porous media by using magnetic resonance imaging.
[So] Source:Magn Reson Imaging;34(7):855-63, 2016 Sep.
[Is] ISSN:1873-5894
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gravity drainage characteristics are important to improve our understanding of gas-liquid or liquid-liquid two-phase flow in porous media. Stable or unstable displacement fronts that controlled by the capillary force, viscous force, gravitational force, etc., are relevant features of immiscible two-phase flow. In this paper, three dimensionless parameters, namely, the gravity number, the capillary number and the Bond number, were used to describe the effect of the above mentioned forces on two-phase drainage features, including the displacement front and final displacing-phase saturation. A series of experiments on the downward displacement of a viscous fluid by a less viscous fluid in a vertical vessel that is filled with quartz beads are performed by using magnetic resonance imaging (MRI). The experimental results indicate that the wetting properties at both high and low capillary numbers exert remarkable control on the fluid displacement. When the contact angle is lower than 90°, i.e., the displaced phase is the wetting phase, the average velocity Vf of the interface of the two phases (displacement front velocity) is observably lower than when the displaced phase is the non-wetting phase (contact angle higher than 90°). The results show that a fingering phenomenon occurs when the gravity number G is less than the critical gravity number G'=Δµ/µg. Moreover, the higher Bond number results in higher final displacing-phase saturation, whereas the capillary number has an opposite effect.
[Mh] Termos MeSH primário: Drenagem
Gravitação
Imagem por Ressonância Magnética/métodos
Porosidade
[Mh] Termos MeSH secundário: Ação Capilar
Viscosidade
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160313
[St] Status:MEDLINE


  8 / 773 MEDLINE  
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[PMID]:26439913
[Au] Autor:Lutsko JF; Nicolis G
[Ad] Endereço:Center for Nonlinear Phenomena and Complex Systems, Université Libre de Bruxelles, Code Postal 231, Blvd. du Triomphe, 1050 Brussels, Belgium. jlutsko@ulb.ac.be.
[Ti] Título:Mechanism for the stabilization of protein clusters above the solubility curve.
[So] Source:Soft Matter;12(1):93-8, 2016 Jan 07.
[Is] ISSN:1744-6848
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pan, Vekilov and Lubchenko [J. Phys. Chem. B, 2010, 114, 7620] have proposed that dense stable protein clusters appearing in weak protein solutions above the solubility curve are composed of protein oligomers. The hypothesis is that a weak solution of oligomer species is unstable with respect to condensation causing the formation of dense, oligomer-rich droplets which are stabilized against growth by the monomer-oligomer reaction. Here, we show that such a combination of processes can be understood using a simple capillary model yielding analytic expressions for the cluster properties which can be used to interpret experimental data. We also construct a microscopic Dynamic Density Functional Theory model and show that it is consistent with the predictions of the capillary model. The viability of the mechanism is thus confirmed and it is shown how the radius of the stable clusters is related to physically interesting quantities such as the monomer-oligomer rate constants.
[Mh] Termos MeSH primário: Multimerização Proteica
[Mh] Termos MeSH secundário: Ação Capilar
Estabilidade Proteica
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151007
[St] Status:MEDLINE
[do] DOI:10.1039/c5sm02234g


  9 / 773 MEDLINE  
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[PMID]:26382133
[Au] Autor:Lee I; Hüttemann M; Malek MH
[Ad] Endereço:1College of Medicine, Dankook University, Cheonan-si, Republic of Korea; 2Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan; 3Cardiovascular Research Institute, Wayne State University School of Medicine, Detroit, Michigan; and 4Integrative Physiology of Exercise Laboratory, Department of Health Care Sciences, Eugene Applebaum College of Pharmacy & Health Sciences, Wayne State University, Detroit, Michigan.
[Ti] Título:(-)-Epicatechin Attenuates Degradation of Mouse Oxidative Muscle Following Hindlimb Suspension.
[So] Source:J Strength Cond Res;30(1):1-10, 2016 Jan.
[Is] ISSN:1533-4287
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to conduct a 14-day hindlimb suspension (HS) with and without (-)-epicatechin supplementation to determine whether (-)-epicatechin treatment can attenuate the loss in muscle degradation, angiogenesis, and mitochondrial signaling in oxidative skeletal muscle. Adult mice were randomized into 3 groups: (a) control (C); (b) HS with vehicle (HS-V); and (c) HS with (-)-epicatechin (HS-(-)-Epi). Animals in the HS-(-)-Epi group received (-)-epicatechin (1.0 mg · kg(-1) of body mass) twice daily through oral gavage. For markers related to muscle degradation, the HS-V group had significantly higher protein expression compared with the control and HS-(-)-Epi groups. Moreover, protein expression for myosin heavy chain type I was significantly reduced by approximately 45% in the HS-V group compared with the control and HS-(-)-Epi groups. In addition, capillarity contact and capillary-to-fiber ratio were significantly higher in the HS-(-)-Epi group compared with the HS-V group. Furthermore, protein expression for thrombospondin-1 was significantly higher in HS-V group compared with the control and HS-(-)-Epi groups. Hindlimb suspension also significantly reduced protein expression for mitochondrial signaling compared with the control and HS-(-)-Epi groups. These findings suggest that (-)-epicatechin supplementation attenuates degradation in oxidative muscles after HS.
[Mh] Termos MeSH primário: Catequina/farmacologia
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/metabolismo
Cadeias Pesadas de Miosina/metabolismo
Miosina Tipo I/metabolismo
[Mh] Termos MeSH secundário: Adaptação Fisiológica/efeitos dos fármacos
Animais
Capilares/efeitos dos fármacos
Capilares/patologia
Capilares/fisiopatologia
Ação Capilar/efeitos dos fármacos
Elevação dos Membros Posteriores/efeitos adversos
Elevação dos Membros Posteriores/fisiologia
Masculino
Camundongos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Músculo Esquelético/patologia
Neovascularização Fisiológica/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
Trombospondina 1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Thrombospondin 1); 0 (thrombospondin-1, mouse); 8R1V1STN48 (Catechin); EC 3.6.1.- (Myosin Type I); EC 3.6.4.1 (Myosin Heavy Chains)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150919
[St] Status:MEDLINE
[do] DOI:10.1519/JSC.0000000000001205


  10 / 773 MEDLINE  
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[PMID]:26340743
[Au] Autor:Liu W; Lanier TC; Osborne JA
[Ad] Endereço:James Ford Bell Technical Center, General Mills, 9000 Plymouth Ave. N, Minneapolis, MN 55427, United States. Electronic address: Wenjie1108@gmail.com.
[Ti] Título:Capillarity proposed as the predominant mechanism of water and fat stabilization in cooked comminuted meat batters.
[So] Source:Meat Sci;111:67-77, 2016 Jan.
[Is] ISSN:1873-4138
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fat- and nonfat-containing meat gels structurally became coarser and porous by partial substitution of whey protein isolate for myofibrillar protein, creating a weaker texture plus greater cook loss (CL: fat+water) and expressible water (EW). Microstructure examinations revealed a tendency for fat to coalesce during cooking of the more coarse-structured gels. This tendency was unaffected by fat pre-emulsification prior to addition, arguing against a strong role of an interfacial protein film in stabilizing fat. Instead, a gel structure with evenly distributed small pores leads to lower CL and EW, thus controlling both water- and fat- holding since fat cannot readily permeate small water-filled hydrophilic pores. Only when large pores or continuous fissures are structurally present can water be released, allowing liquid fat to also migrate and coalesce. This changes the current paradigm of understanding regarding the mechanism of fat/water-holding in comminuted meat products: gel capillarity (gel structure), not fat emulsifying ability of protein, is the likely determining factor.
[Mh] Termos MeSH primário: Culinária
Gorduras na Dieta/análise
Proteínas na Dieta/análise
Manipulação de Alimentos
Produtos da Carne/análise
Modelos Químicos
Água/análise
[Mh] Termos MeSH secundário: Adsorção
Animais
Ação Capilar
Galinhas
Proteínas na Dieta/química
Emulsões
Qualidade dos Alimentos
Géis
Interações Hidrofóbicas e Hidrofílicas
Microscopia Confocal
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Proteínas Musculares/análise
Proteínas Musculares/química
Proteínas Musculares/ultraestrutura
Tamanho da Partícula
Porosidade
Proteínas do Soro do Leite/análise
Proteínas do Soro do Leite/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Dietary Fats); 0 (Dietary Proteins); 0 (Emulsions); 0 (Gels); 0 (Muscle Proteins); 0 (Whey Proteins); 059QF0KO0R (Water); SI6O3IW77Z (lard)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150905
[St] Status:MEDLINE



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