Base de dados : MEDLINE
Pesquisa : G03.143.500 [Categoria DeCS]
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[PMID]:29351552
[Au] Autor:Kropf J; Rössler W
[Ad] Endereço:Behavioral Physiology and Sociobiology (Zoology II), Biozentrum, University of Würzburg, Würzburg, Germany.
[Ti] Título:In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
[So] Source:PLoS One;13(1):e0191425, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The honeybee olfactory pathway comprises an intriguing pattern of convergence and divergence: ~60.000 olfactory sensory neurons (OSN) convey olfactory information on ~900 projection neurons (PN) in the antennal lobe (AL). To transmit this information reliably, PNs employ relatively high spiking frequencies with complex patterns. PNs project via a dual olfactory pathway to the mushroom bodies (MB). This pathway comprises the medial (m-ALT) and the lateral antennal lobe tract (l-ALT). PNs from both tracts transmit information from a wide range of similar odors, but with distinct differences in coding properties. In the MBs, PNs form synapses with many Kenyon cells (KC) that encode odors in a spatially and temporally sparse way. The transformation from complex information coding to sparse coding is a well-known phenomenon in insect olfactory coding. Intrinsic neuronal properties as well as GABAergic inhibition are thought to contribute to this change in odor representation. In the present study, we identified intrinsic neuronal properties promoting coding differences between PNs and KCs using in-situ patch-clamp recordings in the intact brain. We found very prominent K+ currents in KCs clearly differing from the PN currents. This suggests that odor coding differences between PNs and KCs may be caused by differences in their specific ion channel properties. Comparison of ionic currents of m- and l-ALT PNs did not reveal any differences at a qualitative level.
[Mh] Termos MeSH primário: Abelhas/citologia
Abelhas/fisiologia
Condutos Olfatórios/citologia
Condutos Olfatórios/fisiologia
[Mh] Termos MeSH secundário: Potenciais de Ação
Animais
Antenas de Artrópodes/citologia
Antenas de Artrópodes/fisiologia
Encéfalo/citologia
Encéfalo/fisiologia
Fenômenos Eletrofisiológicos
Canais Iônicos/fisiologia
Transporte de Íons
Corpos Pedunculados/citologia
Corpos Pedunculados/fisiologia
Neurônios Receptores Olfatórios/citologia
Neurônios Receptores Olfatórios/fisiologia
Técnicas de Patch-Clamp
Olfato/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ion Channels)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191425


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[PMID]:29360848
[Au] Autor:Liu Z; Zhu J; Yang X; Wu H; Wei Q; Wei H; Zhang H
[Ad] Endereço:Research Center of Saline and Alkali Land of State Forestry Administration, Chinese Academy of Forestry, Beijing, P. R. China.
[Ti] Título:Growth performance, organ-level ionic relations and organic osmoregulation of Elaeagnus angustifolia in response to salt stress.
[So] Source:PLoS One;13(1):e0191552, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Elaeagnus angustifolia is one of the most extensively afforested tree species in environment-harsh regions of northern China. Despite its exceptional tolerance to saline soil, the intrinsic adaptive physiology has not been revealed. In this study, we investigated the growth, organ-level ionic relations and organic osmoregulation of the seedlings hydroponically treated with 0, 100 and 200 mM NaCl for 30 days. We found that the growth characteristics and the whole-plant dry weight were not obviously stunted, but instead, were even slightly stimulated by the treatment of 100 mM NaCl. In contrast, these traits were significantly inhibited by 200 mM NaCl treatment. Interestingly, as compared with the control (0 mM NaCl), both 100 and 200 mM NaCl treatments had a promotional effect on root growth as evidenced by 26.3% and 2.4% increases in root dry weight, respectively. Roots had the highest Na+ and Cl- concentrations and obviously served as the sink for the net increased Na+ and Cl-, while, stems might maintain the capacity of effective Na+ constraint, resulting in reduced Na+ transport to the leaves. K+, Ca2+ and Mg2+ concentrations in three plant organs of NaCl-treated seedlings presented a substantial decline, eventually leading to an enormously drop of K+/Na+ ratio. As the salt concentration increased, proline and soluble protein contents continuously exhibited a prominent and a relatively tardy accumulation, respectively, whereas soluble sugar firstly fell to a significant level and then regained to a level that is close to that of the control. Taken together, our results provided quantitative measures that revealed some robust adaptive physiological mechanisms underpinning E. angustifolia's moderately high salt tolerance, and those mechanisms comprise scalable capacity for root Na+ and Cl- storage, effectively constrained transportation of Na+ from stems to leaves, root compensatory growth, as well as an immediate and prominent leaf proline accumulation.
[Mh] Termos MeSH primário: Elaeagnaceae/efeitos dos fármacos
Osmorregulação
Cloreto de Sódio/farmacologia
Estresse Fisiológico
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Cloretos/metabolismo
Elaeagnaceae/crescimento & desenvolvimento
Elaeagnaceae/fisiologia
Transporte de Íons
Folhas de Planta/metabolismo
Raízes de Plantas/metabolismo
Potássio/metabolismo
Plântulas/metabolismo
Sódio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chlorides); 451W47IQ8X (Sodium Chloride); 9NEZ333N27 (Sodium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191552


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[PMID]:29254313
[Au] Autor:Goren A; Naccarato T; Situm M; Kovacevic M; Lotti T; McCoy J
[Ad] Endereço:Applied Biology, Inc., Irvine, CA, USA.
[Ti] Título:Mechanism of action of minoxidil in the treatment of androgenetic alopecia is likely mediated by mitochondrial adenosine triphosphate synthase-induced stem cell differentiation.
[So] Source:J Biol Regul Homeost Agents;31(4):1049-1053, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Topical minoxidil is the only topical drug approved by the US Food and Drug Administration (FDA) for the treatment of androgenetic alopecia. However, the exact mechanism by which minoxidil stimulates anagen phase and promotes hair growth is not fully understood. In the late telegen phase of the hair follicle growth cycle, stem cells located in the bulge region differentiate and re-enter anagen phase, a period of growth lasting 2-6 years. In androgenetic alopecia, the anagen phase is shortened and a progressive miniaturization of hair follicles occurs, eventually leading to hair loss. Several studies have demonstrated that minoxidil increases the amount of intracellular Ca2+, which has been shown to up-regulate the enzyme adenosine triphosphate (ATP) synthase. A recent study demonstrated that ATP synthase, independent of its role in ATP synthesis, promotes stem cell differentiation. As such, we propose that minoxidil induced Ca2+ influx can increase stem cell differentiation and may be a key factor in the mechanism by which minoxidil facilitates hair growth. Based on our theory, we provide a roadmap for the development of a new class of drugs for the treatment of androgenetic alopecia.
[Mh] Termos MeSH primário: Alopecia/tratamento farmacológico
Folículo Piloso/efeitos dos fármacos
Minoxidil/uso terapêutico
Mitocôndrias/efeitos dos fármacos
ATPases Mitocondriais Próton-Translocadoras/genética
Células-Tronco/efeitos dos fármacos
Vasodilatadores/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Alopecia/enzimologia
Alopecia/genética
Alopecia/patologia
Cálcio/metabolismo
Diferenciação Celular/efeitos dos fármacos
Expressão Gênica
Folículo Piloso/enzimologia
Folículo Piloso/patologia
Seres Humanos
Transporte de Íons/efeitos dos fármacos
Masculino
Meia-Idade
Mitocôndrias/enzimologia
ATPases Mitocondriais Próton-Translocadoras/metabolismo
Células-Tronco/enzimologia
Células-Tronco/patologia
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vasodilator Agents); 5965120SH1 (Minoxidil); EC 3.6.3.- (Mitochondrial Proton-Translocating ATPases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29360451
[Au] Autor:Xu J; Yang F; Han D; Xu S
[Ad] Endereço:Key Laboratory for the Physics & Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing, 100871, PR China.
[Ti] Título:Phenomena of synchronized response in biosystems and the possible mechanism.
[So] Source:Biochem Biophys Res Commun;496(2):661-666, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phenomena of synchronized response is common among organs, tissues and cells in biosystems. We have analyzed and discussed three examples of synchronization in biosystems, including the direction-changing movement of paramecia, the prey behavior of flytraps, and the simultaneous discharge of electric eels. These phenomena and discussions support an electrical communication mechanism that in biosystems, the electrical signals are mainly soliton-like electromagnetic pulses, which are generated by the transient transmembrane ionic current through the ion channels and propagate along the dielectric membrane-based softmaterial waveguide network to complete synchronized responses. This transmission model implies that a uniform electrical communication mechanism might have been naturally developed in biosystem.
[Mh] Termos MeSH primário: Comunicação Animal
Fenômenos Eletrofisiológicos
Canais Iônicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Membrana Celular/metabolismo
Eletricidade
Electrophorus/fisiologia
Transporte de Íons
Movimento
Paramecium/fisiologia
Sarraceniaceae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ion Channels)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE


  5 / 10235 MEDLINE  
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[PMID]:29223393
[Au] Autor:Korotkov SM; Konovalova SA; Nesterov VP; Brailovskaya IV
[Ad] Endereço:Sechenov Institute of Evolutionary Physiology and Biochemistry, The Russian Academy of Sciences, Thorez pr. 44, 194223 St. Petersburg, Russia. Electronic address: korotkov@SK1645.spb.edu.
[Ti] Título:Mersalyl prevents the Tl -induced permeability transition pore opening in the inner membrane of Ca -loaded rat liver mitochondria.
[So] Source:Biochem Biophys Res Commun;495(2):1716-1721, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It was earlier shown that the calcium load of rat liver mitochondria in medium containing TlNO and KNO resulted in the Tl -induced mitochondrial permeability transition pore (MPTP) opening in the inner membrane. This opening was accompanied by an increase in swelling and membrane potential dissipation and a decrease in state 3, state 4, and 2,4-dinitrophenol-uncoupled respiration. This respiratory decrease was markedly leveled by mersalyl (MSL), the phosphate symporter (PiC) inhibitor which poorly stimulated the calcium-induced swelling, but further increased the potential dissipation. All of these effects of Ca and MSL were visibly reduced in the presence of the MPTP inhibitors (ADP, N-ethylmaleimide, and cyclosporine A). High MSL concentrations attenuated the ability of ADP to inhibit the MPTP. Our data suggest that the PiC can participate in the Tl -induced MPTP opening in the inner membrane of Ca -loaded rat liver mitochondria.
[Mh] Termos MeSH primário: Mersalil/farmacologia
Mitocôndrias Hepáticas/efeitos dos fármacos
Mitocôndrias Hepáticas/metabolismo
Proteínas de Transporte da Membrana Mitocondrial/efeitos dos fármacos
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Tálio/farmacologia
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Técnicas In Vitro
Transporte de Íons/efeitos dos fármacos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Membranas Mitocondriais/efeitos dos fármacos
Membranas Mitocondriais/metabolismo
Dilatação Mitocondrial/efeitos dos fármacos
Consumo de Oxigênio/efeitos dos fármacos
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Mitochondrial Membrane Transport Proteins); 0 (mitochondrial permeability transition pore); 5X1IO031V8 (Mersalyl); AD84R52XLF (Thallium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE


  6 / 10235 MEDLINE  
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[PMID]:29298982
[Au] Autor:Li X; Zhai T; Gao P; Cheng H; Hou R; Lou X; Xia F
[Ad] Endereço:State Key Laboratory of Material Processing and Die & Mould Technology, School of Material Sciences and Engineering, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), 430074, Wu
[Ti] Título:Role of outer surface probes for regulating ion gating of nanochannels.
[So] Source:Nat Commun;9(1):40, 2018 01 03.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nanochannels with functional elements have shown promise for DNA sequencing, single-molecule sensing, and ion gating. Ionic current measurement is currently a benchmark, but is focused solely on the contribution from nanochannels' inner-wall functional elements (NIWFE); the attributes of functional elements at nanochannels' outer surface (NOSFE) are nearly ignored, and remain elusive. Here we show that the role of NOSFE and NIWFE for ion gating can be distinguished by constructing DNA architectures using dual-current readout. The established molecular switches have continuously tunable and reversible ion-gating ability. We find that NOSFE exhibits negligible ion-gating behavior, but it can produce a synergistic effect in alliance with NIWFE. Moreover, the high-efficiency gating systems display more noticeable synergistic effect than the low-efficiency ones. We also reveal that the probe amount of NOSFE and NIWFE is almost equally distributed in our biomimetic nanochannels, which is potentially a premise for the synergistic ion-gating phenomena.
[Mh] Termos MeSH primário: Materiais Biomiméticos
Ativação do Canal Iônico
Transporte de Íons
Nanoestruturas
[Mh] Termos MeSH secundário: Óxido de Alumínio
Técnicas de Patch-Clamp
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
LMI26O6933 (Aluminum Oxide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02447-7


  7 / 10235 MEDLINE  
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[PMID]:29179179
[Au] Autor:Shi R; Xiao ZT; Zheng YJ; Zhang YL; Xu JW; Huang JH; Zhou WL; Li PB; Su WW
[Ad] Endereço:Guangdong Engineering & Technology Research Center for Quality and Efficacy Re-evaluation of Post-market Traditional Chinese Medicine, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
[Ti] Título:Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells.
[So] Source:Cell Physiol Biochem;44(3):1146-1160, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Sputum symptoms are commonly seen in the elderly. This study aimed to identify an efficacious expectorant treatment stratagem through evaluating the secretion-promoting activation and cystic fibrosis transmembrane conductance regulator (CFTR) expression of the bioactive herbal monomer naringenin. METHODS: Vectorial Cl- transport was determined by measuring short-circuit current (ISC) in rat airway epithelium. cAMP content was measured by ELISA in primary cultured epithelial cells and Calu-3 cells. CFTR expression in Calu-3 cells was determined by qPCR. RESULTS: Addition of naringenin to the basolateral side of the rat airway led to a concentration-dependent sustained increase in ISC. The current was suppressed when exposed to Cl--free solution or by bumetanide, BaCl2, and DPC but not by DIDS and IBMX. Forskolin-induced ISC increase and CFTRinh-172/MDL-12330A-induced ISC inhibition were not altered by naringenin. Intracellular cAMP content was significantly increased by naringenin. With lipopolysaccharide stimulation, CFTR expression was significantly reduced, and naringenin dose-dependently enhanced CFTR mRNA expression. CONCLUSION: These results demonstrate that naringenin has the ability to stimulate Cl- secretion, which is mediated by CFTR through a signaling pathway by increasing cAMP content. Moreover, naringenin can increase CFTR expression when organism CFTR expression is seriously hampered. Our data suggest a potentially effective treatment strategy for sputum.
[Mh] Termos MeSH primário: Regulador de Condutância Transmembrana em Fibrose Cística/metabolismo
Células Epiteliais/efeitos dos fármacos
Flavanonas/farmacologia
[Mh] Termos MeSH secundário: Animais
Compostos de Bário/farmacologia
Benzoatos/farmacologia
Células Cultivadas
Canais de Cloreto/antagonistas & inibidores
Canais de Cloreto/metabolismo
Cloretos/farmacologia
Colforsina/farmacologia
AMP Cíclico/análise
Regulador de Condutância Transmembrana em Fibrose Cística/antagonistas & inibidores
Regulador de Condutância Transmembrana em Fibrose Cística/genética
Células Epiteliais/citologia
Células Epiteliais/metabolismo
Feminino
Seres Humanos
Iminas/farmacologia
Transporte de Íons/efeitos dos fármacos
Masculino
Microscopia de Fluorescência
Ratos
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
Tiazolidinas/farmacologia
Traqueia/citologia
ortoaminobenzoatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-((3-trifluoromethyl)phenyl)-5-((3-carboxyphenyl)methylene)-2-thioxo-4-thiazolidinone); 0 (Barium Compounds); 0 (Benzoates); 0 (Chloride Channels); 0 (Chlorides); 0 (Flavanones); 0 (Imines); 0 (Thiazolidines); 0 (ortho-Aminobenzoates); 0VK51DA1T2 (barium chloride); 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator); 1F7A44V6OU (Colforsin); 82985-31-7 (RMI 12330A); 952VN06WBB (fenamic acid); E0399OZS9N (Cyclic AMP); HN5425SBF2 (naringenin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1159/000485419


  8 / 10235 MEDLINE  
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[PMID]:29236392
[Au] Autor:Rudnytska MV; Palladina TA
[Ti] Título:Effect of preparations Methyure and Ivine on Са(2+)-ATPases activity in plasma and vacuolar membrane of corn seedling roots under salt stress conditions.
[So] Source:Ukr Biochem J;89(1):76-81, 2017 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Ca2+-ATPases regulate the functioning of Ca2+-dependent signaling pathway SOS which provides removal of Na+ from the cytoplasm of cells via Na+/H+-antiporters in saline conditions. The influence of synthetic preparations Methyure and Ivine on the Ca2+-ATPase activity was investigated. It was shown that exposition of corn seedlings in the presence of 0.1 M NaCl rather enhanced hydrolytic than transport activity of Ca2+-ATPases in plasma and vacuolar membrane of root cells. It was found that seed treatment with such preparations, especially Methyure, caused intensification of the both activities of Ca2+-ATPases, mainly in vacuolar membrane. The results indicate than salt protective activity of preparations, especially Methyure, is associated with increased Ca2+-ATPase activity, which regulates the functioning of Na+/H+-antiporters.
[Mh] Termos MeSH primário: ATPases Transportadoras de Cálcio/metabolismo
Membrana Celular/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Pirimidinas/farmacologia
Trocadores de Sódio-Hidrogênio/metabolismo
Vacúolos/efeitos dos fármacos
Zea mays/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Membrana Celular/metabolismo
Membranas Intracelulares/efeitos dos fármacos
Membranas Intracelulares/metabolismo
Transporte de Íons
Células Vegetais/efeitos dos fármacos
Células Vegetais/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Salinidade
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
Cloreto de Sódio/farmacologia
Trocadores de Sódio-Hidrogênio/agonistas
Estresse Fisiológico
Vacúolos/metabolismo
Zea mays/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protective Agents); 0 (Pyrimidines); 0 (Sodium-Hydrogen Exchangers); 451W47IQ8X (Sodium Chloride); EC 3.6.3.8 (Calcium-Transporting ATPases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.076


  9 / 10235 MEDLINE  
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[PMID]:29235789
[Au] Autor:Danylovych YV; Chunikhin AY; Danylovych GV; Kolomiets OV
[Ti] Título:The use of the Petri net method in the simulation modeling of mitochondrial swelling.
[So] Source:Ukr Biochem J;88(4):66-74, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Using photon correlation spectroscopy, which allows investigating changes in the hydrodynamic dia­meter of the particles in suspension, it was shown that ultrahigh concentrations of Ca2+ (over 10 mM) induce swelling of isolated mitochondria. An increase in hydrodynamic diameter was caused by an increase of non-specific mitochondrial membrane permeability to Ca ions, matrix Ca2+ overload, activation of ATP- and Ca2+-sensitive K+-channels, as well as activation of cyclosporin-sensitive permeability transition pore. To formalize the experimental data and to assess conformity of experimental results with theoretical predictions we developed a simulation model using the hybrid functional Petri net method.
[Mh] Termos MeSH primário: Cálcio/farmacologia
Ciclosporina/farmacologia
Mitocôndrias/efeitos dos fármacos
Dilatação Mitocondrial/efeitos dos fármacos
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Cátions Bivalentes
Permeabilidade da Membrana Celular/efeitos dos fármacos
Simulação por Computador
Feminino
Transporte de Íons
Canais KATP/metabolismo
Cinética
Mitocôndrias/metabolismo
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Miométrio/química
Miométrio/metabolismo
Canais de Potássio Cálcio-Ativados/metabolismo
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations, Divalent); 0 (KATP Channels); 0 (Mitochondrial Membrane Transport Proteins); 0 (Potassium Channels, Calcium-Activated); 0 (mitochondrial permeability transition pore); 83HN0GTJ6D (Cyclosporine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.066


  10 / 10235 MEDLINE  
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[PMID]:29235339
[Au] Autor:Babich LG; Shlykov SG; Kushnarova AM; Kosterin SO
[Ti] Título:Ca(2+)-dependent regulation of the Ca(2+) concentration in the myometrium mitochondria. I. Trifluoperazine effects on mitochondria membranes polarization and [Ca(2+)](m).
[So] Source:Ukr Biochem J;88(4):5-11, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Са2+-dependent regulation of Ca2+ exchange in mitochondria is carried out with participation of calmodulin. We have shown previously that calmodulin antagonists reduced the level of mitochondrial membrane polarization and induced increase of the ionized Са concentration in both the mitochondrial matrix and cell cytoplasm. The concentration-dependent influence of trifluoperazine on the level of polarization of mitochondrial membranes has been shown in this work. The coordinates of the Hill graphs were used to calculate the constant K0.5 and the Hill coefficient. K0.5 was 24.4 ± 5 µM (n = 10). The Hill coefficient was 2.0 ± 0.2, indicating the presence of two centers of the trifluoperazine binding. We have also studied [Ca2+]m changes, when incubating mitochondria in mediums of different composition: without ATP and ions of Mg (0-medium), in the presence of 3 mM Mg (Mg-medium) and 3 mM Mg + 3 mM ATP (Mg,ATP-medium). It was shown that the composition of the incubation medium affected the [Ca2+]m values in the absence of exogenous Ca2+ and did not affect them in the presence of the latter. Preincubation of mitochondria in mediums of different composition with 25 µM trifluoperazine did not affect the [Ca2+]m values both before and after the addition of 100 µÐœ Са2+ to the incubation medium. It was concluded, that trifluoperazine depolarized myometrial mitochondria membranes in concentration-dependent manner. However, mitochondria preincubation with 25 µM trifluope­razine accompanied by 50% decrease in membrane polarization did not affect the [Ca2+]m values.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Membranas Mitocondriais/efeitos dos fármacos
Trifluoperazina/farmacologia
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Animais
Calmodulina/antagonistas & inibidores
Calmodulina/metabolismo
Meios de Cultura/química
Relação Dose-Resposta a Droga
Feminino
Transporte de Íons/efeitos dos fármacos
Cinética
Magnésio/metabolismo
Mitocôndrias/metabolismo
Membranas Mitocondriais/metabolismo
Miométrio/química
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calmodulin); 0 (Culture Media); 214IZI85K3 (Trifluoperazine); 8L70Q75FXE (Adenosine Triphosphate); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.005



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