[PMID]: | 26744469 |
[Au] Autor: | Halliez MC; Motta JP; Feener TD; Guérin G; LeGoff L; François A; Colasse E; Favennec L; Gargala G; Lapointe TK; Altier C; Buret AG |
[Ad] Endereço: | Protozooses transmises par l'alimentation, Rouen University Hospital, University of Rouen and University of Reims Champagne-Ardennes, and Institute for Biomedical Research, Rouen and Reims, France; Department of Biological Sciences, Inflammation Research Network, Host-Parasite Interaction NSERC-CREA |
[Ti] Título: | Giardia duodenalis induces paracellular bacterial translocation and causes postinfectious visceral hypersensitivity. |
[So] Source: | Am J Physiol Gastrointest Liver Physiol;310(8):G574-85, 2016 Apr 15. |
[Is] ISSN: | 1522-1547 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Irritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder. It is characterized by abdominal hypersensitivity, leading to discomfort and pain, as well as altered bowel habits. While it is common for IBS to develop following the resolution of infectious gastroenteritis [then termed postinfectious IBS (PI-IBS)], the mechanisms remain incompletely understood. Giardia duodenalis is a cosmopolitan water-borne enteropathogen that causes intestinal malabsorption, diarrhea, and postinfectious complications. Cause-and-effect studies using a human enteropathogen to help investigate the mechanisms of PI-IBS are sorely lacking. In an attempt to establish causality between giardiasis and postinfectious visceral hypersensitivity, this study describes a new model of PI-IBS in neonatal rats infected with G. duodenalis At 50 days postinfection with G. duodenalis (assemblage A or B), long after the parasite was cleared, rats developed visceral hypersensitivity to luminal balloon distension in the jejunum and rectum, activation of the nociceptive signaling pathway (increased c-fos expression), histological modifications (villus atrophy and crypt hyperplasia), and proliferation of mucosal intraepithelial lymphocytes and mast cells in the jejunum, but not in the rectum. G. duodenalis infection also disrupted the intestinal barrier, in vivo and in vitro, which in turn promoted the translocation of commensal bacteria. Giardia-induced bacterial paracellular translocation in vitro correlated with degradation of the tight junction proteins occludin and claudin-4. The extensive observations associated with gut hypersensitivity described here demonstrate that, indeed, in this new model of postgiardiasis IBS, alterations to the gut mucosa and c-fos are consistent with those associated with PI-IBS and, hence, offer avenues for new mechanistic research in the field. |
[Mh] Termos MeSH primário: |
Microbioma Gastrointestinal Giardíase/complicações Síndrome do Intestino Irritável/etiologia Migração Transcelular de Célula
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[Mh] Termos MeSH secundário: |
Animais Células CACO-2 Escherichia coli/patogenicidade Escherichia coli/fisiologia Feminino Giardíase/microbiologia Seres Humanos Mucosa Intestinal/metabolismo Mucosa Intestinal/patologia Síndrome do Intestino Irritável/microbiologia Síndrome do Intestino Irritável/parasitologia Masculino Nociceptividade Ratos Ratos Sprague-Dawley Proteínas de Junções Íntimas/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Tight Junction Proteins) |
[Em] Mês de entrada: | 1706 |
[Cu] Atualização por classe: | 170605 |
[Lr] Data última revisão:
| 170605 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160109 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1152/ajpgi.00144.2015 |
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