Base de dados : MEDLINE
Pesquisa : G03.171.450 [Categoria DeCS]
Referências encontradas : 5193 [refinar]
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[PMID]:28468837
[Au] Autor:Lu W; Rettenmeier E; Paszek M; Yueh MF; Tukey RH; Trottier J; Barbier O; Chen S
[Ad] Endereço:Laboratory of Environmental Toxicology, Department of Pharmacology, University of California, San Diego, La Jolla, California (W.L., E.R., M.P., M-F.Y., R.H.T., S.C.); and Laboratory of Molecular Pharmacology, CHU de Quebec Research Centre and Faculty of Pharmacy, Laval University, Québec (Québec),
[Ti] Título:Crypt Organoid Culture as an in Vitro Model in Drug Metabolism and Cytotoxicity Studies.
[So] Source:Drug Metab Dispos;45(7):748-754, 2017 Jul.
[Is] ISSN:1521-009X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gastrointestinal tract is enriched with xenobiotic processing proteins that play important roles in xenobiotic bioactivation, metabolism, and detoxification. The application of genetically modified mouse models has been instrumental in characterizing the function of xenobiotic processing genes (XPG) and their proteins in drug metabolism. Here, we report the utilization of three-dimensional crypt organoid cultures from these animal models to study intestinal drug metabolism and toxicity. With the successful culturing of crypt organoids, we profiled the abundance of Phase I and Phase II XPG expression, drug transporter gene expression, and xenobiotic nuclear receptor (XNR) gene expression. Functions of XNRs were examined by treating crypt cells with XNR prototypical agonists. Real-time quantitative polymerase chain reaction demonstrated that the representative downstream target genes were induced. These findings were validated from cultures developed from XNR-null mice. In crypt cultures isolated from mice, pregnenolone 16 -carbonitrile failed to induce gene expression; similarly, WY14643 failed to induce in the crypts. Crypt cultures from control ( ) and intestinal epithelial cell (IEC) specific null mice ( ) were treated with camptothecin-11, an anticancer prodrug with severe intestinal toxicity that originates from insufficient UGT1A1-dependent glucuronidation of its active metabolite SN-38. In the absence of gene expression, crypt cultures exhibit very limited production of SN-38 glucuronide, concordant with increased apoptosis in comparison with crypt cultures. This study suggests crypt organoid cultures as an effective in vitro model for studying intestinal drug metabolism and toxicity.
[Mh] Termos MeSH primário: Camptotecina/análogos & derivados
Inativação Metabólica/fisiologia
Organoides/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Camptotecina/metabolismo
Técnicas de Cultura de Células/métodos
Citocromo P-450 CYP3A/metabolismo
Expressão Gênica/fisiologia
Intestinos/metabolismo
Taxa de Depuração Metabólica/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Xenobióticos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Xenobiotics); 7673326042 (irinotecan); EC 1.14.14.1 (Cytochrome P-450 CYP3A); XT3Z54Z28A (Camptothecin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1124/dmd.117.075945


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[PMID]:28461575
[Au] Autor:Phuc NM; Wu Z; O Y; Lee JH; Oh S; Song GY; Liu KH
[Ad] Endereço:BK21 Plus KNU Multi-Omics-Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, Korea (N.M.P., Z.W., K.-H.L.); College of Pharmacy, Chungnam National University, Daejeon, Korea (Y.O., J.-H.L., G.-Y.S.); and Dep
[Ti] Título:LKY-047: First Selective Inhibitor of Cytochrome P450 2J2.
[So] Source:Drug Metab Dispos;45(7):765-769, 2017 Jul.
[Is] ISSN:1521-009X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Highly selective cytochrome P450 CYP2J2 (CYP2J2) inhibitors suitable for reaction phenotyping are currently not available. (7 )-(+)-(4-Nitro-phenyl)-acrylic acid, 8,8-dimethyl-2-oxo-6,7-dihydro- -pyrano[3,2-g]chromen-7-yl-ester (LKY-047), a decursin derivative, was synthesized, and its inhibitor potencies toward CYP2J2 as well as other cytochrome P450 (P450) enzymes in human liver microsomes (HLM) were evaluated. LKY-047 was demonstrated to be a strong competitive inhibitor of CYP2J2-mediated astemizole -demethylase and terfenadine hydroxylase activity, with values of 0.96 and 2.61 M, respectively. It also acted as an uncompetitive inhibitor of CYP2J2-mediated ebastine hydroxylation with a value of 3.61 M. Preincubation of LKY-047 with HLMs and NADPH did not alter inhibition potency, indicating that it is not a mechanism-based inhibitor. LKY-047 was found to be a selective CYP2J2 inhibitor with no inhibitory effect on other human P450s, such as CYPs 1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, and 3A (IC > 50 M). These in vitro data support the use of LKY-047 as a selective CYP2J2 inhibitor with potential application in the identification of P450 isoforms responsible for drug metabolism in reaction phenotyping assays.
[Mh] Termos MeSH primário: Inibidores das Enzimas do Citocromo P-450/farmacologia
Sistema Enzimático do Citocromo P-450/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
Hidroxilação/efeitos dos fármacos
Inativação Metabólica/efeitos dos fármacos
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/metabolismo
NADP/metabolismo
Isoformas de Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytochrome P-450 Enzyme Inhibitors); 0 (Protein Isoforms); 53-59-8 (NADP); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.14.1 (arachidonate epoxygenase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1124/dmd.117.075036


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[PMID]:28972920
[Au] Autor:Park K; Kwak IS
[Ad] Endereço:Faculty of Marine Technology, Chonnam National University, Yeosu 550-749, South Korea.
[Ti] Título:Disrupting effects of antibiotic sulfathiazole on developmental process during sensitive life-cycle stage of Chironomus riparius.
[So] Source:Chemosphere;190:25-34, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antibiotics in the environment are a concern due to their potential to harm humans and interrupt ecosystems. Sulfathiazole (STZ), a sulfonamide antibiotic, is commonly used in aquaculture and is typically found in aquatic ecosystems. We evaluated the ecological risk of STZ by examining biological, molecular and biochemical response in Chironomus riparius. Samples were exposed to STZ for 12, 24 and 96 h, and effects of STZ were evaluated at the molecular level by analyzing changes in gene expression related to the endocrine system, cellular stress response and enzyme activity of genes on antioxidant and detoxification pathways. STZ exposure induced significant effects on survival, growth and sex ratio of emergent adults and mouthpart deformity in C. riparius. STZ caused concentration and time-dependent toxicity in most of the selected biomarkers. STZ exposure leads to significant heat-shock response of protein genes (HSP70, HSP40, HSP90 and HSP27) and to disruption by up-regulating selected genes, including the ecdysone receptor gene, estrogen-related receptors, ultraspiracle and E74 early ecdysone-responsive gene. Furthermore, STZ induced alteration of enzyme activities on antioxidant and detoxification responses (catalase, superoxide dismutase, glutathione peroxidase and peroxidase) in C. riparius. By inducing oxidative stress, antibiotic STZ disturbs the endocrine system and produces adverse effects in growth processes of invertebrates.
[Mh] Termos MeSH primário: Chironomidae/efeitos dos fármacos
Expressão Gênica/efeitos dos fármacos
Estágios do Ciclo de Vida/efeitos dos fármacos
Sulfatiazóis/toxicidade
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Antibacterianos/toxicidade
Chironomidae/crescimento & desenvolvimento
Ecdisona/metabolismo
Sistema Endócrino/efeitos dos fármacos
Sistema Endócrino/metabolismo
Proteínas de Choque Térmico HSP70/metabolismo
Resposta ao Choque Térmico/efeitos dos fármacos
Inativação Metabólica/efeitos dos fármacos
Receptores de Esteroides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (HSP70 Heat-Shock Proteins); 0 (Receptors, Steroid); 0 (Sulfathiazoles); 0 (ecdysone receptor); 3604-87-3 (Ecdysone); Y7FKS2XWQH (sulfathiazole)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE


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[PMID]:29386454
[Au] Autor:Matsumoto A
[Ad] Endereço:Department of Social Medicine, Saga University School of Medicine.
[Ti] Título:[Importance of an Aldehyde Dehydrogenase 2 Polymorphism in Preventive Medicine].
[So] Source:Nihon Eiseigaku Zasshi;73(1):9-20, 2018.
[Is] ISSN:1882-6482
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Unlike genetic alterations in other aldehyde dehydrogenase (ALDH) isozymes, a defective ALDH2 polymorphism (rs671), which is carried by almost half of East Asians, does not show a clear phenotype such as a shortened life span. However, impacts of a defective ALDH2 allele, ALDH2*2, on various disease risks have been reported. As ALDH2 is responsible for the detoxification of endogenous aldehydes, a negative effect of this polymorphism is predicted, but bidirectional effects have been actually observed and the mechanisms underlying such influences are often complex. One reason for this complexity may be the existence of compensatory aldehyde detoxification systems and the secondary effects of these systems. There are many issues to be addressed with regard to the ALDH2 polymorphism in the field of preventive medicine, including the following concerns. First, ALDH2 in the fetal stage plays a role in aldehyde detoxification; therefore, prenatal health effects of environmental aldehyde exposure are of concern for ALDH2*2-carrying fetuses. Second, ALDH2*2 carriers are at high risk of drinking-related cancers. However, their drinking habits result in less worsening of physiological findings, such as energy metabolism index and liver functions, compared with non-ALDH2*2 carriers, and therefore opportunities to detect excessive drinking can be lost. Third, personalized medicine such as personalized prescriptions for ALDH2*2 carriers will be required in the clinical setting, and accumulation of evidence is awaited. Lastly, since the ALDH2 polymorphism is not considered in workers' limits of exposure to aldehydes and their precursors, efforts to lower exposure levels beyond legal standards are required.
[Mh] Termos MeSH primário: Aldeído-Desidrogenase Mitocondrial/genética
Aldeído-Desidrogenase Mitocondrial/fisiologia
Aldeídos/efeitos adversos
Aldeídos/metabolismo
Estudos de Associação Genética
Inativação Metabólica/genética
Saúde do Trabalhador
Polimorfismo Genético
Medicina Preventiva
[Mh] Termos MeSH secundário: Consumo de Bebidas Alcoólicas/genética
Feminino
Heterozigoto
Seres Humanos
Isoenzimas/genética
Isoenzimas/fisiologia
Estilo de Vida
Exposição Materna/efeitos adversos
Troca Materno-Fetal
Exposição Ocupacional/efeitos adversos
Exposição Ocupacional/prevenção & controle
Gravidez
Risco
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Aldehydes); 0 (Isoenzymes); EC 1.2.1.3 (ALDH2 protein, human); EC 1.2.1.3 (Aldehyde Dehydrogenase, Mitochondrial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1265/jjh.73.9


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[PMID]:28923727
[Au] Autor:Labade CP; Jadhav AR; Ahire M; Zinjarde SS; Tamhane VA
[Ad] Endereço:Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, (SPPU), Ganeshkhind Road, Pune 411007, Maharashtra, India.
[Ti] Título:Role of induced glutathione-S-transferase from Helicoverpa armigera (Lepidoptera: Noctuidae) HaGST-8 in detoxification of pesticides.
[So] Source:Ecotoxicol Environ Saf;147:612-621, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study deals with glutathione-S-transferase (GST) based detoxification of pesticides in Helicoverpa armigera and its potential application in eliminating pesticides from the environment. Dietary exposure of a pesticide mixture (organophosphates - chlorpyrifos and dichlorvos, pyrethroid - cypermethrin; 2-15ppm each) to H. armigera larvae resulted in a dose dependant up-regulation of GST activity and gene expression. A variant GST from H. armigera (HaGST-8) was isolated from larvae fed with 10ppm pesticide mixture and it was recombinantly expressed in yeast (Pichia pastoris HaGST-8). HaGST-8 had a molecular mass of 29kDa and was most active at pH 9 at 30°C. GC-MS and LC-HRMS analysis validated that HaGST-8 was effective in eliminating organophosphate type of pesticides and partially reduced the cypermethrin content (53%) from aqueous solutions. Unlike the untransformed yeast, P. pastoris HaGST-8 grew efficiently in media supplemented with pesticide mixtures (200 and 400ppm each pesticide) signifying the detoxification ability of HaGST-8. The amino acid sequence of HaGST-8 and the already reported sequence of HaGST-7 had just 2 mismatches. The studies on molecular interaction strengths revealed that HaGST-8 had stronger binding affinities with organophosphate, pyrethroid, organochloride, carbamate and neonicotinoid type of pesticides. The abilities of recombinant HaGST-8 to eliminate pesticides and P. pastoris HaGST-8 to grow profusely in the presence of high level of pesticide content can be applied for removal of such residues from food, water resources and bioremediation.
[Mh] Termos MeSH primário: Glutationa Transferase/biossíntese
Lepidópteros/enzimologia
Praguicidas/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Animais
Biodegradação Ambiental
Relação Dose-Resposta a Droga
Expressão Gênica
Glutationa Transferase/genética
Inativação Metabólica
Cinética
Larva/efeitos dos fármacos
Larva/enzimologia
Lepidópteros/efeitos dos fármacos
Praguicidas/toxicidade
Poluentes do Solo/toxicidade
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticides); 0 (Soil Pollutants); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


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[PMID]:28455184
[Au] Autor:Schweizer F; Heidel-Fischer H; Vogel H; Reymond P
[Ad] Endereço:Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland.
[Ti] Título:Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore.
[So] Source:Insect Biochem Mol Biol;85:21-31, 2017 06.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytophagous insects have to deal with toxic defense compounds from their host plants. Although it is known that insects have evolved genes and mechanisms to detoxify plant allochemicals, how specialist and generalist precisely respond to specific secondary metabolites at the molecular level is less understood. Here we studied the larval performance and transcriptome of the generalist moth Heliothis virescens and the specialist butterfly Pieris brassicae feeding on Arabidopsis thaliana genotypes with different glucosinolate (GS) levels. H. virescens larvae gained significantly more weight on the GS-deficient mutant quadGS compared to wild-type (Col-0) plants. On the contrary, P. brassicae was unaffected by the presence of GS and performed equally well on both genotypes. Strikingly, there was a considerable differential gene expression in H. virescens larvae feeding on Col-0 compared to quadGS. In contrast, compared to H. virescens, P. brassicae displayed a much-reduced transcriptional activation when fed on both plant genotypes. Transcripts coding for putative detoxification enzymes were significantly upregulated in H. virescens, along with digestive enzymes and transposable elements. These data provide an unprecedented view on transcriptional changes that are specifically activated by GS and illustrate differential molecular responses that are linked to adaptation to diet in lepidopteran herbivores.
[Mh] Termos MeSH primário: Borboletas/efeitos dos fármacos
Glucosinolatos/farmacologia
Herbivoria
Mariposas/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Arabidopsis
Borboletas/genética
Borboletas/metabolismo
Regulação para Baixo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Inativação Metabólica/genética
Proteínas de Insetos/metabolismo
Larva/efeitos dos fármacos
Mariposas/genética
Mariposas/metabolismo
Análise de Sequência de RNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucosinolates); 0 (Insect Proteins); 0 (cuticle proteins, insects); 0 (storage proteins, Insecta)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29030272
[Au] Autor:Rasmussen MK; Bertholdt L; Gudiksen A; Pilegaard H; Knudsen JG
[Ad] Endereço:Department of Food Science, Aarhus University, Blichers alle 20, P.O. Box 50, DK-8830 Tjele, Denmark. Electronic address: Martink.rasmussen@food.au.dk.
[Ti] Título:Impact of fasting followed by short-term exposure to interleukin-6 on cytochrome P450 mRNA in mice.
[So] Source:Toxicol Lett;282:93-99, 2018 Jan 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The gene expression of the cytochrome P450 (CYP) enzyme family is regulated by numerous factors. Fasting has been shown to induce increased hepatic CYP mRNA in both humans and animals. However, the coordinated regulation of CYP, CYP-regulating transcription factors, and transcriptional co-factors in the liver linking energy metabolism to detoxification has never been investigated. Interleukin-6 (IL-6) has been suggested to be released during fasting and has been shown to regulate CYP expression. The present study investigated the hepatic mRNA content of selected CYP, AhR, CAR, PXR and PPARα in mice fasted for 18h and subsequently exposed to IL-6. Furthermore, the impact of fasting on PGC-1α, HNF-4α, SIRT1 and SIRT3 mRNA was examined. Fasting induced a marked increase in Cyp2b10, Cyp2e1 and Cyp4a10 mRNA, while CYP1a1, Cyp1a2, Cyp2a4 and Cyp3a11 mRNA levels remained unchanged. In accordance, the mRNA levels of CAR and PPARα were also increased with fasting. The PGC-1α, SIRT1 and SIRT3 mRNA levels were also increased after fasting, while the HNF-4α mRNA levels remained unchanged. In mice subjected to IL-6 injection, the fasting-induced PXR, PPARα and PGC-1α mRNA responses were lower than after saline injection. In conclusion, fasting was demonstrated to be a strong inducer of hepatic CYP mRNA as well as selected transcription factors controlling the expression of the investigated CYP. Moreover, the mRNA levels of transcriptional co-factors acting as energy sensors and co-factors for CYP regulation was also increased in the liver, suggesting crosstalk at the molecular level between regulation of energy metabolism and detoxification.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Metabolismo Energético
Jejum/metabolismo
Interleucina-6/farmacologia
Fígado/metabolismo
RNA Mensageiro/metabolismo
[Mh] Termos MeSH secundário: Animais
Sistema Enzimático do Citocromo P-450/genética
Metabolismo Energético/efeitos dos fármacos
Inativação Metabólica
Interleucina-6/sangue
Fígado/efeitos dos fármacos
Masculino
Camundongos Endogâmicos C57BL
RNA Mensageiro/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-6); 0 (RNA, Messenger); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171015
[St] Status:MEDLINE


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[PMID]:28842127
[Au] Autor:Arbildi P; Turell L; López V; Alvarez B; Fernández V
[Ad] Endereço:Cátedra de Inmunología/DEPBIO, Facultad de Química, Universidad de la República, Montevideo, 11600, Uruguay.
[Ti] Título:Mechanistic insights into EgGST1, a Mu class glutathione S-transferase from the cestode parasite Echinococcus granulosus.
[So] Source:Arch Biochem Biophys;633:15-22, 2017 Nov 01.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glutathione transferases (GSTs) comprise a major detoxification system in helminth parasites, displaying both catalytic and non-catalytic activities. The kinetic mechanism of these enzymes is complex and depends on the isoenzyme which is being analyzed. Here, we characterized the kinetic mechanism of rEgGST1, a recombinant form of a cytosolic GST from Echinococcus granulosus (EgGST1), which is related to the Mu-class of mammalian enzymes, using the canonical substrates glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). Initial rate and product inhibition studies were consistent with a steady-state random sequential mechanism, where both substrates are bound to the enzyme before the products are released. Kinetic constants were also determined (pH 6.5 and 30 °C). Moreover, rEgGST1 lowered the pK of GSH from 8.71 ± 0.07 to 6.77 ± 0.08, and enzyme-bound GSH reacted with CDNB 1 × 10 times faster than free GSH at pH 7.4. Finally, the dissociation of the enzyme-GSH complex was studied by means of intrinsic fluorescence, as well as that of the complex with the anthelminth drug mebendazole. This is the first report on mechanistic issues related to a helminth parasitic GST.
[Mh] Termos MeSH primário: Echinococcus granulosus/química
Glutationa Transferase/metabolismo
Glutationa/metabolismo
Proteínas de Helminto/metabolismo
Proteínas Recombinantes de Fusão/metabolismo
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/farmacologia
Clonagem Molecular
Dinitroclorobenzeno/metabolismo
Echinococcus granulosus/enzimologia
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Glutationa Transferase/antagonistas & inibidores
Glutationa Transferase/genética
Proteínas de Helminto/antagonistas & inibidores
Proteínas de Helminto/genética
Concentração de Íons de Hidrogênio
Inativação Metabólica/genética
Isoenzimas/genética
Isoenzimas/metabolismo
Cinética
Mebendazol/farmacologia
Ligação Proteica
Proteínas Recombinantes de Fusão/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Helminth Proteins); 0 (Isoenzymes); 0 (Recombinant Fusion Proteins); 81G6I5V05I (Mebendazole); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE


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[PMID]:28767647
[Au] Autor:Petit G; Luminet O; Cordovil de Sousa Uva M; Zorbas A; Maurage P; de Timary P
[Ad] Endereço:Research Institute for Psychological Sciences, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
[Ti] Título:Differential spontaneous recovery across cognitive abilities during detoxification period in alcohol-dependence.
[So] Source:PLoS One;12(8):e0176638, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: There is a lack of consensus regarding the extent to which cognitive dysfunctions may recover upon cessation of alcohol intake by alcohol-dependents (AD), and the divergent findings are most likely due to methodological differences between the various studies. The present study was aimed at conducting a very strict longitudinal study of cognitive recovery in terms of assessment points, the duration of abstinence, control of age and duration of the addiction, and by use of individual analyses in addition to mean group comparisons. Our study further focused on the 2-3 week phase of alcohol detoxification that is already known to positively affect many biological, emotional, motivational, as well as neural variables, followed by longer-term therapies for which good cognitive functioning is needed. METHODS: 41 AD inpatients undergoing a detoxification program, and 41 matched controls, were evaluated twice in terms of five cognitive functions (i.e., short-term memory, working memory, inhibition, cognitive flexibility, and verbal fluency) within a three-week interval [on the first day (T1) and the 18th day (T2) of abstinence for AD patients]. Emotional (positive and negative affectivity and depression) and motivational (craving) variables were also measured at both evaluation times. RESULTS: Although verbal fluency, short-term memory, and cognitive flexibility did not appear to be affected, the patients exhibited impaired inhibition and working memory at T1. While no recovery of inhibition was found to occur, the average working memory performance of the patients was comparable to that of the controls at T2. Improvements in emotional and motivational dimensions were also observed, although they did not correlate with the ones in working memory. Individual analysis showed that not all participants were impaired or recover the same functions. CONCLUSIONS: While inhibition deficits appear to persist after 18 days of detoxification, deficits in working memory, which is a central component of cognition, are greatly reduced after alcohol detoxification. Individual differences in the trajectory of recovery do arise however, and it might be worth implementing individual assessments of impaired functions at the end of the detoxification phase in order to maximize the chances of success in longer-term treatments and abstinence.
[Mh] Termos MeSH primário: Alcoolismo/fisiopatologia
Cognição/fisiologia
[Mh] Termos MeSH secundário: Adulto
Alcoolismo/tratamento farmacológico
Benzodiazepinas/uso terapêutico
Estudos de Casos e Controles
Fissura
Emoções
Feminino
Seres Humanos
Inativação Metabólica
Estudos Longitudinais
Masculino
Memória de Curto Prazo
Meia-Idade
Testes Neuropsicológicos
Avaliação de Programas e Projetos de Saúde
Tempo de Reação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
12794-10-4 (Benzodiazepines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176638


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[PMID]:28753905
[Au] Autor:Chen X; Zhang W; Luo X; Zhao F; Li Y; Li R; Li Z
[Ad] Endereço:College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
[Ti] Título:Efficient removal and environmentally benign detoxification of Cr(VI) in aqueous solutions by Zr(IV) cross-linking chitosan magnetic microspheres.
[So] Source:Chemosphere;185:991-1000, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Zirconium(IV) cross-linking chitosan (CTS) magnetic microspheres (Fe O @Zr-CTS) as a recoverable adsorbent were synthesized through the coordination reaction between zirconium oxychloride and CTS biopolymeric matrix for efficient adsorption and simultaneous detoxification of hexavalent chromium, Cr(VI), in aqueous solutions. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) confirmed the formation of core@shell magnetite microspheres. X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) verified the crosslinking of Zr(IV) to CTS on the microspheres. Batch Cr(VI) adsorption performances of the resultant Fe O @Zr-CTS microspheres revealed that the maximum adsorption capacity of 280.97 mg/g were achieved under pH 4.0 at 298 K. The XPS analyses indicated that 61.1% of the adsorbed Cr(VI) was reduced to Cr(III) due to the oxidization of alcoholic groups on C-6 in CTS which served as electron donors to carbonyl groups. The adsorbent showed preferential Cr(VI) adsorption with the existence of co-existing cations (K , Na , Cu , Zn , Ca , Mg ) and anions (NO , Cl , SO , CO ). The adsorbent exhibited excellent reusability, lower the effluent Cr(VI) contents down to the ppb level, which satisfied the drinking water standard recommended by the World Health Organization and was a promising candidate for water purification.
[Mh] Termos MeSH primário: Quitosana/química
Cromo/metabolismo
Microesferas
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Adsorção
Cromo/análise
Concentração de Íons de Hidrogênio
Inativação Metabólica
Cinética
Magnetismo
Oxirredução
Espectroscopia Fotoeletrônica
Espectroscopia de Infravermelho com Transformada de Fourier
Poluentes Químicos da Água/análise
Poluentes Químicos da Água/química
Purificação da Água/métodos
Difração de Raios X
Zircônio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium); 18540-29-9 (chromium hexavalent ion); 9012-76-4 (Chitosan); C6V6S92N3C (Zirconium); T4294601O7 (zirconium oxychloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170730
[St] Status:MEDLINE



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