Base de dados : MEDLINE
Pesquisa : G04.144.220.220 [Categoria DeCS]
Referências encontradas : 209 [refinar]
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  1 / 209 MEDLINE  
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[PMID]:28245054
[Au] Autor:Chikashige Y; Yamane M; Okamasa K; Osakada H; Tsutsumi C; Nagahama Y; Fukuta N; Haraguchi T; Hiraoka Y
[Ad] Endereço:Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
[Ti] Título:Fission yeast APC/C activators Slp1 and Fzr1 sequentially trigger two consecutive nuclear divisions during meiosis.
[So] Source:FEBS Lett;591(7):1029-1040, 2017 Apr.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In meiosis, two rounds of nuclear division occur consecutively without DNA replication between the divisions. We isolated a fission yeast mutant in which the nucleus divides only once to generate two spores, as opposed to four, in meiosis. In this mutant, we found that the initiation codon of the slp1 gene is converted to ATA, producing a reduced amount of Slp1. As a member of the Fizzy family of anaphase-promoting complex/cyclosome (APC/C) activators, Slp1 is essential for vegetative growth; however, the mutant allele shows a phenotype only in meiosis. Slp1 insufficiency delays degradation of maturation-promoting factor at the first meiotic division, and another APC/C activator, Fzr1, which acts late in meiosis, terminates meiosis immediately after the delayed first division to produce two viable spores.
[Mh] Termos MeSH primário: Proteínas Cdc20/metabolismo
Proteínas Cdh1/metabolismo
Meiose
Proteínas de Schizosaccharomyces pombe/metabolismo
Schizosaccharomyces/metabolismo
[Mh] Termos MeSH secundário: Ciclossomo-Complexo Promotor de Anáfase/metabolismo
Western Blotting
Proteínas Cdc20/genética
Proteínas Cdh1/genética
Divisão do Núcleo Celular/genética
Microscopia de Fluorescência
Mutação
Schizosaccharomyces/genética
Proteínas de Schizosaccharomyces pombe/genética
Esporos Fúngicos/genética
Esporos Fúngicos/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Cdc20 Proteins); 0 (Cdh1 Proteins); 0 (Schizosaccharomyces pombe Proteins); 0 (slp1 protein, S pombe); EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12612


  2 / 209 MEDLINE  
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[PMID]:28125974
[Au] Autor:Shipman EN; Jones K; Jenkinson CB; Kim DW; Zhu J; Khang CH
[Ad] Endereço:Department of Plant Biology, University of Georgia, Athens, GA, 30602, USA.
[Ti] Título:Nuclear and structural dynamics during the establishment of a specialized effector-secreting cell by Magnaporthe oryzae in living rice cells.
[So] Source:BMC Cell Biol;18(1):11, 2017 Jan 26.
[Is] ISSN:1471-2121
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To cause an economically important blast disease on rice, the filamentous fungus Magnaporthe oryzae forms a specialized infection structure, called an appressorium, to penetrate host cells. Once inside host cells, the fungus produces a filamentous primary hypha that differentiates into multicellular bulbous invasive hyphae (IH), which are surrounded by a host-derived membrane. These hyphae secrete cytoplasmic effectors that enter host cells presumably via the biotrophic interfacial complex (BIC). The first IH cell, also known as the side BIC-associated cell, is a specialized effector-secreting cell essential for a successful infection. This study aims to determine cellular processes that lead to the development of this effector-secreting first IH cell inside susceptible rice cells. RESULTS: Using live-cell confocal imaging, we determined a series of cellular events by which the appressorium gives rise to the first IH cell in live rice cells. The filamentous primary hypha extended from the appressorium and underwent asymmetric swelling at its apex. The single nucleus in the appressorium divided, and then one nucleus migrated into the swollen apex. Septation occurred in the filamentous region of the primary hypha, establishing the first IH cell. The tip BIC that was initially associated with the primary hypha became the side BIC on the swollen apex prior to nuclear division in the appressorium. The average distance between the early side BIC and the nearest nucleus in the appressorium was estimated to be more than 32 µm. These results suggest an unknown mechanism by which effectors that are expressed in the appressorium are transported through the primary hypha for their secretion into the distantly located BIC. When M. oryzae was inoculated on heat-killed rice cells, penetration proceeded as normal, but there was no differentiation of a bulbous IH cell, suggesting its specialization for establishment of biotrophic infection. CONCLUSIONS: Our studies reveal cellular dynamics associated with the development of the effector-secreting first IH cell. Our data raise new mechanistic questions concerning hyphal differentiation in response to host environmental cues and effector trafficking from the appressorium to the BIC.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Magnaporthe/citologia
Oryza/microbiologia
Células Vegetais/microbiologia
[Mh] Termos MeSH secundário: Morte Celular
Divisão do Núcleo Celular
Temperatura Alta
Hifas/citologia
Mitose
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1186/s12860-017-0126-z


  3 / 209 MEDLINE  
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[PMID]:27458047
[Au] Autor:Nakazawa N; Mehrotra R; Arakawa O; Yanagida M
[Ad] Endereço:G0 Cell Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa, 904-0495, Japan. nakazawa@oist.jp.
[Ti] Título:ICRF-193, an anticancer topoisomerase II inhibitor, induces arched telophase spindles that snap, leading to a ploidy increase in fission yeast.
[So] Source:Genes Cells;21(9):978-93, 2016 Sep.
[Is] ISSN:1365-2443
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:ICRF-193 [meso-4,4-(2,3-butanediyl)-bis(2,6-piperazinedione)] is a complex-stabilizing inhibitor of DNA topoisomerase II (topo II) that is used as an effective anticancer drug. ICRF-193 inhibits topo II catalytic activity in vitro and blocks nuclear division in vivo. Here, we examined the effects of ICRF-193 treatment on chromatin behavior and spindle dynamics using detailed live mitotic cell analysis in the fission yeast, Schizosaccharomyces pombe. Time-lapse movie analysis showed that ICRF-193 treatment leads to an elongation of presumed chromatin fibers connected to kinetochores during mid-mitosis. Anaphase spindles begin to arch, and eventually spindle poles come together abruptly, as if the spindle snapped at the point of spindle microtubule overlap in telophase. Segregating chromosomes appeared as elastic clumps and subsequently pulled back and merged. The snapped spindle phenotype was abolished by microtubule destabilization after thiabendazole treatment, accompanied by unequal chromosome segregation or severe defects in spindle extension. Thus, we conclude that ICRF-193-treated, unseparated sister chromatids pulling toward opposite spindle poles produce the arched and snapped telophase spindle. ICRF-193 treatment increased DNA content, suggesting that the failure of sister chromatids to separate properly in anaphase, causes the spindle to break in telophase, resulting in polyploidization.
[Mh] Termos MeSH primário: Piperazinas/farmacologia
Schizosaccharomyces/efeitos dos fármacos
Fuso Acromático/efeitos dos fármacos
Telófase/efeitos dos fármacos
[Mh] Termos MeSH secundário: Anáfase/efeitos dos fármacos
Anáfase/fisiologia
Antineoplásicos/farmacologia
Proteínas de Ciclo Celular/genética
Divisão do Núcleo Celular
Cromátides/efeitos dos fármacos
Cromátides/genética
Cromátides/metabolismo
Segregação de Cromossomos
Cinetocoros/metabolismo
Microtúbulos/efeitos dos fármacos
Mitose
Ploidias
Schizosaccharomyces/genética
Schizosaccharomyces/metabolismo
Proteínas de Schizosaccharomyces pombe/genética
Proteínas de Schizosaccharomyces pombe/metabolismo
Fuso Acromático/fisiologia
Telófase/fisiologia
Inibidores da Topoisomerase II/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cell Cycle Proteins); 0 (Piperazines); 0 (Schizosaccharomyces pombe Proteins); 0 (Topoisomerase II Inhibitors); 21416-68-2 (4,4'-(1,2-dimethyl-1,2-ethanediyl)bis-2,6-piperazinedione)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE
[do] DOI:10.1111/gtc.12397


  4 / 209 MEDLINE  
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[PMID]:27387218
[Au] Autor:Chen A; Xie Q; Lin Y; Xu H; Shang W; Zhang J; Zhang D; Zheng W; Li G; Wang Z
[Ad] Endereço:Key Laboratory of Biopesticides and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Chin
[Ti] Título:Septins are involved in nuclear division, morphogenesis and pathogenicity in Fusarium graminearum.
[So] Source:Fungal Genet Biol;94:79-87, 2016 09.
[Is] ISSN:1096-0937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Septins are GTP-binding proteins that regulate cell polarity, cytokinesis and cell morphogenesis. Fusarium head blight (FHB), caused by Fusarium graminearum, is one of the most devastating diseases worldwide. In this study, we have functionally characterized the core septins, Cdc3, Cdc10, Cdc11 and Cdc12 in F. graminearum. The loss of FgCdc3, FgCdc11, FgCdc12, but not FgCdc10, mutants showed significant reduction in growth, conidiation and virulence. Microscopic analyses revealed that all of them were involved in septum formation and nuclear division. Moreover, disruption of septin genes resulted in morphological defects in ascospores and conidia. Interestingly, conidia produced by ΔFgcdc3, ΔFgcdc11 and ΔFgcdc12 mutants exhibited deformation with interconnecting conidia in contrast to their parent wild-type strain PH-1 and the ΔFgcdc10 mutant that produced normal conidia. Using yeast two-hybrid assays, we determined the interactions among FgCdc3, FgCdc10, FgCdc11 and FgCdc12. Taken together, our results indicate that septins play important roles in the nuclear division, morphogenesis and pathogenicity in F. graminearum.
[Mh] Termos MeSH primário: Divisão do Núcleo Celular
Fusarium/fisiologia
Septinas/fisiologia
[Mh] Termos MeSH secundário: Fusarium/genética
Fusarium/patogenicidade
Deleção de Genes
Genes Fúngicos
Morfogênese
Doenças das Plantas/microbiologia
Septinas/genética
Esporos Fúngicos/crescimento & desenvolvimento
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.6.1.- (Septins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE


  5 / 209 MEDLINE  
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[PMID]:27260975
[Au] Autor:Terzaghi L; Tessaro I; Raucci F; Merico V; Mazzini G; Garagna S; Zuccotti M; Franciosi F; Lodde V
[Ad] Endereço:a Reproductive and Developmental Biology Laboratory, Department of Health , Animal Science and Food Safety, University of Milan , Milan , Italy.
[Ti] Título:PGRMC1 participates in late events of bovine granulosa cells mitosis and oocyte meiosis.
[So] Source:Cell Cycle;15(15):2019-32, 2016 Aug 02.
[Is] ISSN:1551-4005
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Progesterone Receptor Membrane Component 1 (PGRMC1) is expressed in both oocyte and ovarian somatic cells, where it is found in multiple cellular sub-compartments including the mitotic spindle apparatus. PGRMC1 localization in the maturing bovine oocytes mirrors its localization in mitotic cells, suggesting a possible common action in mitosis and meiosis. To test the hypothesis that altering PGRMC1 activity leads to similar defects in mitosis and meiosis, PGRMC1 function was perturbed in cultured bovine granulosa cells (bGC) and maturing oocytes and the effect on mitotic and meiotic progression assessed. RNA interference-mediated PGRMC1 silencing in bGC significantly reduced cell proliferation, with a concomitant increase in the percentage of cells arrested at G2/M phase, which is consistent with an arrested or prolonged M-phase. This observation was confirmed by time-lapse imaging that revealed defects in late karyokinesis. In agreement with a role during late mitotic events, a direct interaction between PGRMC1 and Aurora Kinase B (AURKB) was observed in the central spindle at of dividing cells. Similarly, treatment with the PGRMC1 inhibitor AG205 or PGRMC1 silencing in the oocyte impaired completion of meiosis I. Specifically the ability of the oocyte to extrude the first polar body was significantly impaired while meiotic figures aberration and chromatin scattering within the ooplasm increased. Finally, analysis of PGRMC1 and AURKB localization in AG205-treated oocytes confirmed an altered localization of both proteins when meiotic errors occur. The present findings demonstrate that PGRMC1 participates in late events of both mammalian mitosis and oocyte meiosis, consistent with PGRMC1's localization at the mid-zone and mid-body of the mitotic and meiotic spindle.
[Mh] Termos MeSH primário: Células da Granulosa/citologia
Células da Granulosa/metabolismo
Meiose
Mitose
Oócitos/citologia
Receptores de Progesterona/metabolismo
[Mh] Termos MeSH secundário: Animais
Aurora Quinase B/metabolismo
Bovinos
Divisão do Núcleo Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Feminino
Inativação Gênica/efeitos dos fármacos
Células da Granulosa/efeitos dos fármacos
Meiose/efeitos dos fármacos
Mitose/efeitos dos fármacos
Oócitos/efeitos dos fármacos
Oócitos/metabolismo
Corpos Polares/citologia
Corpos Polares/efeitos dos fármacos
Corpos Polares/metabolismo
Ligação Proteica/efeitos dos fármacos
Tiazóis/farmacologia
Imagem com Lapso de Tempo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AG205); 0 (Receptors, Progesterone); 0 (Thiazoles); EC 2.7.11.1 (Aurora Kinase B)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160605
[St] Status:MEDLINE
[do] DOI:10.1080/15384101.2016.1192731


  6 / 209 MEDLINE  
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[PMID]:27219063
[Au] Autor:Moschou PN; Gutierrez-Beltran E; Bozhkov PV; Smertenko A
[Ad] Endereço:Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, PO Box 7080, 75007 Uppsala, Sweden. Electronic address: panagiotis.moschou@slu.se.
[Ti] Título:Separase Promotes Microtubule Polymerization by Activating CENP-E-Related Kinesin Kin7.
[So] Source:Dev Cell;37(4):350-361, 2016 May 23.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microtubules play an essential role in breaking cellular symmetry. We have previously shown that separase associates with microtubules and regulates microtubule-dependent establishment of cell polarity in Arabidopsis. However, separase lacks microtubule-binding activity, raising questions about mechanisms underlying this phenomenon. Here we report that the N-terminal non-catalytic domain of separase binds to the C-terminal tail domain of three homologs of the centromeric protein CENP-E Kinesin 7 (Kin7). Conformational changes of Kin7 induced upon binding to separase facilitate recruitment of Kin7/separase complex (KISC) onto microtubules. KISC operates independently of proteolytic activity of separase in promoting microtubule rescue and pauses, as well as in suppressing catastrophes. Genetic complementation experiments in conditional separase mutant rsw4 background demonstrate the importance of KISC for the establishment of cell polarity and for plant development. Our study establishes a mechanism governing microtubule dynamics via the separase-dependent activation of CENP-E-related kinesins.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Enzimas/metabolismo
Cinesina/metabolismo
Microtúbulos/metabolismo
Polimerização
Separase/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/química
Biocatálise
Divisão do Núcleo Celular
Polaridade Celular
Gravitropismo
Morfogênese
Complexos Multiproteicos/metabolismo
Ligação Proteica
Domínios Proteicos
Proteólise
Homologia de Sequência de Aminoácidos
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (Enzymes); 0 (Multiprotein Complexes); 0 (centromere protein E); 0 (epithiospecifier protein, Arabidopsis); EC 3.4.22.49 (Separase); EC 3.6.4.4 (Kinesin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE


  7 / 209 MEDLINE  
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[PMID]:27193301
[Au] Autor:Dundon SE; Chang SS; Kumar A; Occhipinti P; Shroff H; Roper M; Gladfelter AS
[Ad] Endereço:Department of Biological Sciences, Dartmouth College, Hanover, NH 03755.
[Ti] Título:Clustered nuclei maintain autonomy and nucleocytoplasmic ratio control in a syncytium.
[So] Source:Mol Biol Cell;27(13):2000-7, 2016 Jul 01.
[Is] ISSN:1939-4586
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nuclei in syncytia found in fungi, muscles, and tumors can behave independently despite cytoplasmic translation and the homogenizing potential of diffusion. We use a dynactin mutant strain of the multinucleate fungus Ashbya gossypii with highly clustered nuclei to assess the relative contributions of nucleus and cytoplasm to nuclear autonomy. Remarkably, clustered nuclei maintain cell cycle and transcriptional autonomy; therefore some sources of nuclear independence function even with minimal cytosol insulating nuclei. In both nuclear clusters and among evenly spaced nuclei, a nucleus' transcriptional activity dictates local cytoplasmic contents, as assessed by the localization of several cyclin mRNAs. Thus nuclear activity is a central determinant of the local cytoplasm in syncytia. Of note, we found that the number of nuclei per unit cytoplasm was identical in the mutant to that in wild-type cells, despite clustered nuclei. This work demonstrates that nuclei maintain autonomy at a submicrometer scale and simultaneously maintain a normal nucleocytoplasmic ratio across a syncytium up to the centimeter scale.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Células Gigantes/metabolismo
[Mh] Termos MeSH secundário: Ciclo Celular/fisiologia
Núcleo Celular/fisiologia
Divisão do Núcleo Celular/fisiologia
Ciclinas/metabolismo
Citoplasma/metabolismo
Citoplasma/patologia
Proteínas Fúngicas/metabolismo
Fungos/metabolismo
Células Gigantes/fisiologia
Mitose
Saccharomycetales/metabolismo
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclins); 0 (Fungal Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1091/mbc.E16-02-0129


  8 / 209 MEDLINE  
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[PMID]:27141899
[Au] Autor:Teng JJ; Yang TJ; Ye L; Feng XQ; Zheng YX; Duan HW
[Ad] Endereço:Key Lab of Chemical Safty and Health, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
[Ti] Título:[Analysis on the nuclear division index of peripheral blood lymphocytes in the 281 general population of Anhui, China].
[So] Source:Zhonghua Yu Fang Yi Xue Za Zhi;50(5):429-33, 2016 May.
[Is] ISSN:0253-9624
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the reference range and influeing factors of the nuclear division index (NDI) of peripheral blood lymphocyte in Chinese general population in Anhui province. METHODS: We selected 281 subjects from the general poulation in Anhui province, without occupational exposure to genetic toxicants and no chronic disease history. We used questionnaires to collect occupational history, age, gender, region, body mass index, smoking, and alcohol drinking status etc. NDI was measured by cytokinesis block micronucleus assay in peripheral blood lymphocytes, and the related factors were also analyzed. And NDI was used as the dependent variable, age, gender and other factors as independent variables to conduct stepwise multiple linear regression. RESULTS: We found the data of NDI according with normal distribution, and the nuclear division index was 1.71±0.22, the minimum value was 1.10 while the maximum was 2.36. The results showed that NDI value of the males (1.67±0.20) were lower than that of the females (1.76±0.24), the difference was statistically significant (t=-3.65, P<0.001); current smokers NDI (1.66±0.18) lower than non-smokers (1.73±0.24) differences were statistically significant (t=3.06, P=0.002); the NDI of drinking groups (1.66±0.20) was lower than that of non-drinking population (1.74±0.23), the differences was statistically significant (t=3.15, P=0.002); Using multiple stepwise linear regression calibration factors and found that gender was an independent factor of NDI (ß=0.098, Sx=0.027, t=3.66, P< 0.001). CONCLUSION: We set the reference value on the nuclear division index among general population of survey areas in this study, it could provide a reference for similar studies and will provide reference for better evaluation of the effects of hazards on the body.
[Mh] Termos MeSH primário: Divisão do Núcleo Celular
Citocinese/efeitos dos fármacos
Linfócitos/patologia
Testes para Micronúcleos/métodos
Valores de Referência
[Mh] Termos MeSH secundário: Consumo de Bebidas Alcoólicas
China/epidemiologia
Feminino
Seres Humanos
Linfócitos/efeitos dos fármacos
Masculino
Fumar/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170111
[Lr] Data última revisão:
170111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160505
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0253-9624.2016.05.008


  9 / 209 MEDLINE  
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[PMID]:27092766
[Au] Autor:Beinke C; Port M; Riecke A; Ruf CG; Abend M
[Ad] Endereço:a Bundeswehr Institute of Radiobiology, 80937 Munich, Germany;
[Ti] Título:Adaption of the Cytokinesis-Block Micronucleus Cytome Assay for Improved Triage Biodosimetry.
[So] Source:Radiat Res;185(5):461-72, 2016 May.
[Is] ISSN:1938-5404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of this work was to adapt a more advanced form of the cytokinesis-block micronucleus (CBMN) cytome assay for triage biodosimetry in the event of a mass casualty radiation incident. We modified scoring procedures for the CBMN cytome assay to optimize field deployability, dose range, accuracy, speed, economy, simplicity and stability. Peripheral blood of 20 donors was irradiated in vitro (0-6 Gy X ray, maximum photon energy 240 keV) and processed for CBMN. Initially, we assessed two manual scoring strategies for accuracy: 1. Conventional scoring, comprised of micronucleus (MN) frequency per 1,000 binucleated (BN) cells (MN/1,000 BN cells); and 2. Evaluation of 1,000, 2,000 and 3,000 cells in total and different cellular subsets based on MN formation and proliferation (e.g., BN cells with and without MN, mononucleated cells). We used linear and logistic regression models to identify the cellular subsets related closest to dose with the best discrimination ability among different doses/dose categories. We validated the most promising subsets and their combinations with 16 blind samples covering a dose range of 0-8.3 Gy. Linear dose-response relationships comparable to the conventional CBMN assay (r(2) = 0.86) were found for BN cells with MN (r(2) = 0.84) and BN cells without MN (r(2) = 0.84). Models of combined cell counts (CCC) of BN cells with and without MN (BN(+MN) and BN(-MN)) with mononucleated cells (Mono) improved this relationship (r(2) = 0.92). Conventional CBMN discriminated dose categories up to 3 Gy with a concordance between 0.96-1.0 upon scoring 1,000 total cells. In 1,000 BN cells, concordances were observed for conventional CBMN up to 4 Gy as well as BN(+MN) or BN(-MN) (about 0.85). At doses of 4-6 Gy, the concordance of conventional CBMN, BN(+MN) and BN(-MN) declined (about 0.55). We found about 20% higher concordance and more precise dose estimates of irradiated and blinded samples for CCC (Mono + BN(+MN)) after scoring 1,000 total cells. Blinded sample analysis revealed that the mean absolute difference (MAD) of dose estimates and the number of dose estimates outside the ±0.5 Gy interval based on CCC (Mono + BN(+MN)) was comparable to conventional CBMN for doses ≤4 Gy when scoring 3,000 total cells or more. At doses >4-8.3 Gy, the MAD of CCC (Mono + BN(+MN)) declined to half of the MADs observed for conventional CBMN, suggesting that the combined cell counts approach improved the discrimination ability. Conventional CBMN at 1,000 total-cell counts performed as efficiently as counting 1,000 BN cells. Discriminating and counting only BN cells with and without MN after 1,000 BN cells at ≤4 Gy revealed performances similar to conventional CBMN. After 3,000 total cells were scored, CCC (Mono + BN(+MN)) was superior to conventional CBMN at >4 Gy up to about 8 Gy. Our modification of CBMN evaluations saved time compared to the widely established semiautomated MN scoring and extended the dose range up to approximately 6 Gy for triage biodosimetry.
[Mh] Termos MeSH primário: Citocinese/efeitos da radiação
Testes para Micronúcleos/métodos
Radiometria/métodos
[Mh] Termos MeSH secundário: Adulto
Apoptose/efeitos da radiação
Divisão do Núcleo Celular/efeitos da radiação
Relação Dose-Resposta à Radiação
Feminino
Seres Humanos
Masculino
Meia-Idade
Necrose/etiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE
[do] DOI:10.1667/RR14294.1


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[PMID]:26945052
[Au] Autor:Toda E; Ohnishi Y; Okamoto T
[Ad] Endereço:Department of Biological Sciences, Tokyo Metropolitan University, Minami-osawa, Hachioji, Tokyo 192-0397, Japan (E.T., Y.O., T.O.).
[Ti] Título:Development of Polyspermic Rice Zygotes.
[So] Source:Plant Physiol;171(1):206-14, 2016 May.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fertilization is a general feature of eukaryotic uni- and multicellular organisms to restore a diploid genome from female and male gamete haploid genomes. In most animals and fucoid algae, polyspermy block occurs at the plasmogamy step. Because the polyspermy barrier in animals and in fucoid algae is incomplete, polyspermic zygotes are generated by multiple fertilization events. However, these polyspermic zygotes with extra centrioles from multiple sperms show aberrant nuclear and cell division. In angiosperms, polyspermy block functions in the egg cell and the central cell to promote faithful double fertilization, although the mechanism of polyspermy block remains unclear. In contrast to the case in animals and fucoid algae, polyspermic zygotes formed in angiosperms are not expected to die because angiosperms lack centrosomes. However, there have been no reports on the developmental profiles of polyspermic zygotes at cellular level in angiosperms. In this study, we produced polyspermic rice zygotes by electric fusion of an egg cell with two sperm cells, and monitored their developmental profiles. Two sperm nuclei and an egg nucleus fused into a zygotic nucleus, and the triploid zygote divided into a two-celled embryo via mitotic division with a typical bipolar microtubule spindle, as observed during mitosis of a diploid zygote. The two-celled proembryos further developed and regenerated into triploid plants. These findings suggest that polyspermic plant zygotes have the potential to form triploid embryos. Polyspermy in angiosperms might be a pathway for the formation of triploid plants, which can contribute significantly to the formation of autopolyploids.
[Mh] Termos MeSH primário: Fertilização/fisiologia
Oryza/citologia
Oryza/embriologia
Oryza/genética
Zigoto/citologia
Zigoto/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Divisão Celular
Fusão Celular/métodos
Núcleo Celular/metabolismo
Divisão do Núcleo Celular/fisiologia
Cromatina/metabolismo
Diploide
Citometria de Fluxo
Microtúbulos/metabolismo
Mitose
Oryza/fisiologia
Sementes/embriologia
Triploidia
Zigoto/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160306
[St] Status:MEDLINE
[do] DOI:10.1104/pp.15.01953



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