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Pesquisa : G04.144.220.220.687.883.750 [Categoria DeCS]
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[PMID]:28848076
[Au] Autor:Sciurano RB; De Luca G; Rahn IM; Solari AJ
[Ad] Endereço:2da. U.A. Biología Celular, Histología, Embriología y Genética, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
[Ti] Título:The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization.
[So] Source:Cytogenet Genome Res;152(3):137-147, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The heteromorphic X and Y chromosomes behave in a special way in mammalian spermatocytes; they form the XY body and synapse only partially. The aim of this article was to study the origin and the role of the special differentiations in the XY pair of the domestic cat during pachytene by analyzing its fine structural characteristics and the immunolocalization of the main meiotic proteins SYCP3, SYCP1, SYCE3, SMC3, γ-H2AX, BRCA1, H3K27me3, and MLH1. The cat XY body shows particularly striking structures: an extreme degree of axial fibrillation in late pachynema and a special location of SYCP3-containing fibrils, bridging different regions of the main X axis, as well as one bridge at the inner end of the pairing region that colocalizes with the single mandatory MLH1 focus. There are sequential changes, first bullous expansions, then subdivision into fibrils, all involving axial thickening. The chromatin of the XY body presents the usual features of meiotic sex chromosome inactivation. An analysis of the XY body of many eutherians and metatherians suggests that axial thickenings are primitive features. The sequential changes in the mass and location of SYCP3-containing fibers vary among the clades because of specific processes of axial assembly/disassembly occurring in different species.
[Mh] Termos MeSH primário: Gatos/genética
Proteínas Nucleares/metabolismo
Estágio Paquíteno/genética
Complexo Sinaptonêmico/metabolismo
Cromossomo X/metabolismo
Cromossomo X/ultraestrutura
Cromossomo Y/metabolismo
Cromossomo Y/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Proteína BRCA1/genética
Proteína BRCA1/metabolismo
Cromatina/metabolismo
Cromatina/ultraestrutura
Histonas/genética
Histonas/metabolismo
Masculino
Microscopia de Fluorescência
Proteína 1 Homóloga a MutL/genética
Proteína 1 Homóloga a MutL/metabolismo
Espermatócitos/metabolismo
Complexo Sinaptonêmico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BRCA1 Protein); 0 (Chromatin); 0 (Histones); 0 (Nuclear Proteins); EC 3.6.1.3 (MutL Protein Homolog 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1159/000479569


  2 / 209 MEDLINE  
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[PMID]:28844861
[Au] Autor:Rinaldi VD; Bolcun-Filas E; Kogo H; Kurahashi H; Schimenti JC
[Ad] Endereço:Cornell University, Departments of Biomedical Sciences and Molecular Biology and Genetics, Ithaca, NY 14850, USA.
[Ti] Título:The DNA Damage Checkpoint Eliminates Mouse Oocytes with Chromosome Synapsis Failure.
[So] Source:Mol Cell;67(6):1026-1036.e2, 2017 Sep 21.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pairing and synapsis of homologous chromosomes during meiosis is crucial for producing genetically normal gametes and is dependent upon repair of SPO11-induced double-strand breaks (DSBs) by homologous recombination. To prevent transmission of genetic defects, diverse organisms have evolved mechanisms to eliminate meiocytes containing unrepaired DSBs or unsynapsed chromosomes. Here we show that the CHK2 (CHEK2)-dependent DNA damage checkpoint culls not only recombination-defective mouse oocytes but also SPO11-deficient oocytes that are severely defective in homolog synapsis. The checkpoint is triggered in oocytes that accumulate a threshold level of spontaneous DSBs (∼10) in late prophase I, the repair of which is inhibited by the presence of HORMAD1/2 on unsynapsed chromosome axes. Furthermore, Hormad2 deletion rescued the fertility of oocytes containing a synapsis-proficient, DSB repair-defective mutation in a gene (Trip13) required for removal of HORMADs from synapsed chromosomes, suggesting that many meiotic DSBs are normally repaired by intersister recombination in mice.
[Mh] Termos MeSH primário: Quinase do Ponto de Checagem 2/metabolismo
Pareamento Cromossômico
Dano ao DNA
Meiose
Oócitos/enzimologia
[Mh] Termos MeSH secundário: ATPases Associadas a Diversas Atividades Celulares
Adenosina Trifosfatases/genética
Adenosina Trifosfatases/metabolismo
Animais
Proteínas de Ciclo Celular/genética
Proteínas de Ciclo Celular/metabolismo
Morte Celular
Quinase do Ponto de Checagem 2/genética
Endodesoxirribonucleases/deficiência
Endodesoxirribonucleases/genética
Feminino
Fertilidade
Genótipo
Infertilidade Feminina/enzimologia
Infertilidade Feminina/genética
Infertilidade Feminina/patologia
Camundongos Endogâmicos C3H
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Oócitos/patologia
Estágio Paquíteno
Fenótipo
Reparo de DNA por Recombinação
Fatores de Tempo
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (HORMAD2 protein, mouse); 0 (Nohma protein, mouse); EC 2.7.1.11 (Checkpoint Kinase 2); EC 2.7.11.1 (Chek2 protein, mouse); EC 3.1.- (Endodeoxyribonucleases); EC 3.1.- (meiotic recombination protein SPO11); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities); EC 3.6.4.- (Trip13 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


  3 / 209 MEDLINE  
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[PMID]:28729426
[Au] Autor:Garcia-Fabiani MB; Montanaro MA; Stringa P; Lacunza E; Cattaneo ER; Santana M; Pellon-Maison M; Gonzalez-Baro MR
[Ad] Endereço:Instituto de Investigaciones Bioquímicas de La Plata 'Rodolfo R. Brenner', Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata 1900, Argentina.
[Ti] Título:Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis.
[So] Source:Biochem J;474(18):3093-3107, 2017 Aug 30.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear. The aim of the present study was to confirm the hypothesis that GPAT2 is required for the normal physiology of testes and male germ cell maturation. The gene was silenced by inoculating lentiviral particles carrying the sequence of a short-hairpin RNA targeting mRNA into mouse testis. Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness were also observed, and the analysis of apoptosis-related gene expression demonstrated the activation of apoptosis in -silenced germ cells. These findings indicate that GPAT2 protein is necessary for the normal development of male gonocytes, and that its absence triggers apoptotic mechanisms, thereby decreasing the number of dividing germ cells.
[Mh] Termos MeSH primário: Glicerol-3-Fosfato O-Aciltransferase/metabolismo
Túbulos Seminíferos/metabolismo
Espermatogênese
Espermatozoides/enzimologia
[Mh] Termos MeSH secundário: Animais
Apoptose
Proteínas Reguladoras de Apoptose/genética
Proteínas Reguladoras de Apoptose/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Glicerol-3-Fosfato O-Aciltransferase/antagonistas & inibidores
Glicerol-3-Fosfato O-Aciltransferase/genética
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Células HEK293
Seres Humanos
Masculino
Camundongos Endogâmicos BALB C
Microscopia de Fluorescência
Estágio Paquíteno
Interferência de RNA
RNA Mensageiro/metabolismo
RNA Interferente Pequeno
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Túbulos Seminíferos/citologia
Túbulos Seminíferos/crescimento & desenvolvimento
Espermatozoides/citologia
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (Recombinant Proteins); 147336-22-9 (Green Fluorescent Proteins); EC 2.3.1.15 (Glycerol-3-Phosphate O-Acyltransferase); EC 2.3.1.15 (glycerol-3-phosphate acyltransferase 2, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1042/BCJ20161018


  4 / 209 MEDLINE  
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[PMID]:28617799
[Au] Autor:Marcet-Ortega M; Pacheco S; Martínez-Marchal A; Castillo H; Flores E; Jasin M; Keeney S; Roig I
[Ad] Endereço:Genome Integrity and Instability Group, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.
[Ti] Título:p53 and TAp63 participate in the recombination-dependent pachytene arrest in mouse spermatocytes.
[So] Source:PLoS Genet;13(6):e1006845, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To protect germ cells from genomic instability, surveillance mechanisms ensure meiosis occurs properly. In mammals, spermatocytes that display recombination defects experience a so-called recombination-dependent arrest at the pachytene stage, which relies on the MRE11 complex-ATM-CHK2 pathway responding to unrepaired DNA double-strand breaks (DSBs). Here, we asked if p53 family members-targets of ATM and CHK2-participate in this arrest. We bred double-mutant mice combining a mutation of a member of the p53 family (p53, TAp63, or p73) with a Trip13 mutation. Trip13 deficiency triggers a recombination-dependent response that arrests spermatocytes in pachynema before they have incorporated the testis-specific histone variant H1t into their chromatin. We find that deficiency for either p53 or TAp63, but not p73, allowed spermatocytes to progress further into meiotic prophase despite the presence of numerous unrepaired DSBs. Even so, the double mutant spermatocytes apoptosed at late pachynema because of sex body deficiency; thus p53 and TAp63 are dispensable for arrest caused by sex body defects. These data affirm that recombination-dependent and sex body-deficient arrests occur via genetically separable mechanisms.
[Mh] Termos MeSH primário: Meiose/genética
Fosfoproteínas/genética
Recombinação Genética
Transativadores/genética
Proteína Supressora de Tumor p53/genética
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Pontos de Checagem do Ciclo Celular
Cromatina/genética
Quebras de DNA de Cadeia Dupla
Reparo do DNA/genética
Histonas/genética
Masculino
Camundongos
Estágio Paquíteno/genética
Espermatócitos/crescimento & desenvolvimento
Espermatócitos/metabolismo
Testículo/crescimento & desenvolvimento
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Histones); 0 (Phosphoproteins); 0 (Trans-Activators); 0 (Trp63 protein, mouse); 0 (Tumor Suppressor Protein p53)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006845


  5 / 209 MEDLINE  
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[PMID]:28297694
[Au] Autor:Torgasheva AA; Borodin PM
[Ad] Endereço:Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, and Novosibirsk State University, Novosibirsk, Russia.
[Ti] Título:Immunocytological Analysis of Meiotic Recombination in the Gray Goose (Anser anser).
[So] Source:Cytogenet Genome Res;151(1):27-35, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Studies on mammals demonstrate wide interspecific variation in the number and distribution of recombination events along chromosomes. Birds represent an interesting model group for comparative analysis of cytological and ecological drivers of recombination rate evolution. Yet, data on variation in recombination rates in birds are limited to a dozen of species. In this study, we used immunolocalization of MLH1, a mismatch repair protein marking mature recombination nodules, to estimate the overall recombination rate and distribution of crossovers along macrochromosomes in female and male meiosis of the gray goose (Anser anser). The average number of MLH1 foci was significantly higher in oocytes than in spermatocytes (73.6 ± 7.8 and 58.9 ± 7.6, respectively). MLH1 foci distribution along individual macrobivalents showed subtelomeric peaks, which were more pronounced in males. Analysis of distances between neighboring MLH1 foci on macrobivalents revealed stronger crossover interference in male meiosis. These data create a framework for future genetic and physical mapping of the gray goose.
[Mh] Termos MeSH primário: Gansos/genética
Recombinação Homóloga
Meiose/genética
Oócitos/metabolismo
Espermatócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/metabolismo
Pareamento Cromossômico
Segregação de Cromossomos
Cromossomos/genética
Troca Genética
Feminino
Gansos/metabolismo
Imuno-Histoquímica
Cariótipo
Masculino
Proteína 1 Homóloga a MutL/metabolismo
Estágio Paquíteno
Complexo Sinaptonêmico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Avian Proteins); EC 3.6.1.3 (MutL Protein Homolog 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1159/000458741


  6 / 209 MEDLINE  
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[PMID]:27665783
[Au] Autor:Rong M; Matsuda A; Hiraoka Y; Lee J
[Ad] Endereço:Laboratory of Developmental Biotechnology, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
[Ti] Título:Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes.
[So] Source:J Reprod Dev;62(6):623-630, 2016 Dec 20.
[Is] ISSN:1348-4400
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Cohesins containing a meiosis-specific α-kleisin subunit, RAD21L or REC8, play roles in diverse aspects of meiotic chromosome dynamics including formation of axial elements (AEs), assembly of the synaptonemal complex (SC), recombination of homologous chromosomes (homologs), and cohesion of sister chromatids. However, the exact functions of individual α-kleisins remain to be elucidated. Here, we examined the localization of RAD21L and REC8 within the SC by super-resolution microscopy, 3D-SIM. We found that both RAD21L and REC8 were localized at the connection sites between lateral elements (LEs) and transverse filaments (TFs) of pachynema with RAD21L locating interior to REC8 sites. RAD21L and REC8 were not symmetrical in terms of synaptic homologs, suggesting that the arrangement of different cohesins is not strictly fixed along all chromosome axes. Intriguingly, some RAD21L signals, but not REC8 signals, were observed between unsynapsed regions of AEs of zygonema as if they formed a bridge between homologs. Furthermore, the signals of recombination intermediates overlapped with those of RAD21L to a greater degree than with those of REC8. These results highlight the different properties of two meiotic α-kleisins, and strongly support the previous proposition that RAD21L is an atypical cohesin that establishes the association between homologs rather than sister chromatids.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Meiose/fisiologia
Proteínas Nucleares/metabolismo
Fosfoproteínas/metabolismo
Espermatócitos/metabolismo
Complexo Sinaptonêmico/metabolismo
[Mh] Termos MeSH secundário: Animais
Masculino
Camundongos
Estágio Paquíteno/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (Nuclear Proteins); 0 (Phosphoproteins); 0 (RAD21L protein, mouse); 0 (Rec8 protein, mouse)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE
[do] DOI:10.1262/jrd.2016-127


  7 / 209 MEDLINE  
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[PMID]:27431324
[Au] Autor:Xu Z; Song Z; Li G; Tu H; Liu W; Liu Y; Wang P; Wang Y; Cui X; Liu C; Shang Y; de Rooij DG; Gao F; Li W
[Ad] Endereço:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation.
[So] Source:Nucleic Acids Res;44(20):9681-9697, 2016 Nov 16.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Meiotic recombination is essential for fertility in most sexually reproducing species, but the molecular mechanisms underlying this process remain poorly understood in mammals. Here, we show that RNF20-mediated H2B ubiquitination is required for meiotic recombination. A germ cell-specific knockout of the H2B ubiquitination E3 ligase RNF20 results in complete male infertility. The Stra8-Rnf20 spermatocytes arrest at the pachytene stage because of impaired programmed double-strand break (DSB) repair. Further investigations reveal that the depletion of RNF20 in the germ cells affects chromatin relaxation, thus preventing programmed DSB repair factors from being recruited to proper positions on the chromatin. The gametogenetic defects of the H2B ubiquitination deficient cells could be partially rescued by forced chromatin relaxation. Taken together, our results demonstrate that RNF20/Bre1p-mediated H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation, and suggest an old drug may provide a new way to treat some oligo- or azoospermia patients with chromatin relaxation disorders.
[Mh] Termos MeSH primário: Montagem e Desmontagem da Cromatina
Cromatina/genética
Cromatina/metabolismo
Histonas/metabolismo
Meiose
Recombinação Genética
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/deficiência
Animais
Quebras de DNA de Cadeia Dupla
Reparo do DNA
Feminino
Células Germinativas/metabolismo
Infertilidade Masculina/genética
Masculino
Camundongos
Camundongos Knockout
Estágio Paquíteno/genética
Espermatócitos/metabolismo
Espermatogênese/genética
Ubiquitina-Proteína Ligases/deficiência
Ubiquitinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Chromatin); 0 (Histones); 0 (Stra8 protein, mouse); EC 2.3.2.27 (RNF20 protein, mouse); EC 2.3.2.27 (Ubiquitin-Protein Ligases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE


  8 / 209 MEDLINE  
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[PMID]:27115519
[Au] Autor:Pigozzi MI; Del Priore L
[Ad] Endereço:INBIOMED-Instituto de Investigaciones Biomédicas, UBA, CONICET, Facultad de Medicina (UBA), Paraguay 2155, Piso 10, C1121ABG, Buenos Aires, Argentina. mpigozzi@fmed.uba.ar.
[Ti] Título:Meiotic recombination analysis in female ducks (Anas platyrhynchos).
[So] Source:Genetica;144(3):307-12, 2016 Jun.
[Is] ISSN:1573-6857
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Meiotic recombination in female ducks was directly studied by immunolocalization of MLH1 protein, a mismatch repair protein of mature recombination nodules. In total, 6820 crossovers were scored along the autosomal synaptonemal complexes in 122 meiotic nuclei. From this analysis we predict that the female map length of the duck is 2845 cM, with a genome wide recombination rate of 2 cM/Mb. MLH1-focus mapping along the six largest bivalents shows regional variations of recombination frequencies that can be linked to differences in chromosome morphology. From this MLH1 mapping it can be inferred that distally located markers will appear more separated in genetic maps than physically equidistant markers located near the centromeres on bivalents 1 and 2. Instead, markers at interstitial positions on the acrocentric bivalents 3-6 will appear more tightly linked than expected on the basis of their physical distance because recombination is comparatively lower at the mid region of these chromosomes. The present results provide useful information to complement linkage mapping in ducks and extend previous knowledge about the variation of recombination rates among domestic Galloanserae.
[Mh] Termos MeSH primário: Patos/genética
Meiose/genética
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Mapeamento Cromossômico
Cromossomos
Troca Genética
Feminino
Ligação Genética
Genoma
Proteína 1 Homóloga a MutL/genética
Estágio Paquíteno/genética
Complexo Sinaptonêmico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.1.3 (MutL Protein Homolog 1)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160427
[St] Status:MEDLINE
[do] DOI:10.1007/s10709-016-9899-9


  9 / 209 MEDLINE  
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[PMID]:27103161
[Au] Autor:Hernández-Hernández A; Masich S; Fukuda T; Kouznetsova A; Sandin S; Daneholt B; Höög C
[Ad] Endereço:Department of Cell and Molecular Biology, Karolinska Institutet Berzelius väg 35, Stockholm 171 77, Sweden.
[Ti] Título:The central element of the synaptonemal complex in mice is organized as a bilayered junction structure.
[So] Source:J Cell Sci;129(11):2239-49, 2016 06 01.
[Is] ISSN:1477-9137
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The synaptonemal complex transiently stabilizes pairing interactions between homologous chromosomes during meiosis. Assembly of the synaptonemal complex is mediated through integration of opposing transverse filaments into a central element, a process that is poorly understood. We have, here, analyzed the localization of the transverse filament protein SYCP1 and the central element proteins SYCE1, SYCE2 and SYCE3 within the central region of the synaptonemal complex in mouse spermatocytes using immunoelectron microscopy. Distribution of immuno-gold particles in a lateral view of the synaptonemal complex, supported by protein interaction data, suggest that the N-terminal region of SYCP1 and SYCE3 form a joint bilayered central structure, and that SYCE1 and SYCE2 localize in between the two layers. We find that disruption of SYCE2 and TEX12 (a fourth central element protein) localization to the central element abolishes central alignment of the N-terminal region of SYCP1. Thus, our results show that all four central element proteins, in an interdependent manner, contribute to stabilization of opposing N-terminal regions of SYCP1, forming a bilayered transverse-filament-central-element junction structure that promotes synaptonemal complex formation and synapsis.
[Mh] Termos MeSH primário: Complexo Sinaptonêmico/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Cromossômicas não Histona/metabolismo
Cromossomos de Mamíferos/metabolismo
Camundongos Endogâmicos C57BL
Modelos Biológicos
Proteínas Nucleares/química
Proteínas Nucleares/metabolismo
Estágio Paquíteno
Ligação Proteica
Complexo Sinaptonêmico/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (Nuclear Proteins); 0 (Sycp1 protein, mouse); 0 (TEX12 protein, mouse)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE
[do] DOI:10.1242/jcs.182477


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[PMID]:27094866
[Au] Autor:da Cruz I; Rodríguez-Casuriaga R; Santiñaque FF; Farías J; Curti G; Capoano CA; Folle GA; Benavente R; Sotelo-Silveira JR; Geisinger A
[Ad] Endereço:Department of Genomics, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, 11,600, Montevideo, Uruguay.
[Ti] Título:Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage.
[So] Source:BMC Genomics;17:294, 2016 Apr 19.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Spermatogenesis is a complex differentiation process that involves the successive and simultaneous execution of three different gene expression programs: mitotic proliferation of spermatogonia, meiosis, and spermiogenesis. Testicular cell heterogeneity has hindered its molecular analyses. Moreover, the characterization of short, poorly represented cell stages such as initial meiotic prophase ones (leptotene and zygotene) has remained elusive, despite their crucial importance for understanding the fundamentals of meiosis. RESULTS: We have developed a flow cytometry-based approach for obtaining highly pure stage-specific spermatogenic cell populations, including early meiotic prophase. Here we combined this methodology with next generation sequencing, which enabled the analysis of meiotic and postmeiotic gene expression signatures in mouse with unprecedented reliability. Interestingly, we found that a considerable number of genes involved in early as well as late meiotic processes are already on at early meiotic prophase, with a high proportion of them being expressed only for the short time lapse of lepto-zygotene stages. Besides, we observed a massive change in gene expression patterns during medium meiotic prophase (pachytene) when mostly genes related to spermiogenesis and sperm function are already turned on. This indicates that the transcriptional switch from meiosis to post-meiosis takes place very early, during meiotic prophase, thus disclosing a higher incidence of post-transcriptional regulation in spermatogenesis than previously reported. Moreover, we found that a good proportion of the differential gene expression in spermiogenesis corresponds to up-regulation of genes whose expression starts earlier, at pachytene stage; this includes transition protein-and protamine-coding genes, which have long been claimed to switch on during spermiogenesis. In addition, our results afford new insights concerning X chromosome meiotic inactivation and reactivation. CONCLUSIONS: This work provides for the first time an overview of the time course for the massive onset and turning off of the meiotic and spermiogenic genetic programs. Importantly, our data represent a highly reliable information set about gene expression in pure testicular cell populations including early meiotic prophase, for further data mining towards the elucidation of the molecular bases of male reproduction in mammals.
[Mh] Termos MeSH primário: Estágio Paquíteno/genética
Espermatogênese/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Sequenciamento de Nucleotídeos em Larga Escala
Masculino
Prófase Meiótica I/genética
Camundongos
Reprodutibilidade dos Testes
Análise de Sequência de RNA
Espermatogônias/citologia
Cromossomo X/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160421
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-2618-1



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