[PMID]: | 29373585 |
[Au] Autor: | Espitia O; Chatelais M; Steenman M; Charrier C; Maurel B; Georges S; Houlgatte R; Verrecchia F; Ory B; Lamoureux F; Heymann D; Gouëffic Y; Quillard T |
[Ad] Endereço: | INSERM, UMR 1238, Nantes, France; Université de Nantes, Nantes Atlantique Universités, Laboratoire « Sarcome osseux et remodelage des tissus osseux calcifiés ¼, Faculté de Médecine, Nantes, France. |
[Ti] Título: | Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification. |
[So] Source: | PLoS One;13(1):e0191976, 2018. |
[Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Vascular calcification is a strong and independent predictive factor for cardiovascular complications and mortality. Our previous work identified important discrepancies in plaque composition and calcification types between carotid and femoral arteries. The objective of this study is to further characterize and understand the heterogeneity in vascular calcification among vascular beds, and to identify molecular mechanisms underlying this process. We established ECLAGEN biocollection that encompasses human atherosclerotic lesions and healthy arteries from different locations (abdominal, thoracic aorta, carotid, femoral, and infrapopliteal arteries) for histological, cell isolation, and transcriptomic analysis. Our results show that lesion composition differs between these locations. Femoral arteries are the most calcified arteries overall. They develop denser calcifications (sheet-like, nodule), and are highly susceptible to osteoid metaplasia. These discrepancies may derive from intrinsic differences between SMCs originating from these locations, as microarray analysis showed specific transcriptomic profiles between primary SMCs isolated from each arterial bed. These molecular differences translated into functional disparities. SMC from femoral arteries showed the highest propensity to mineralize due to an increase in basal TGFß signaling. Our results suggest that biological heterogeneity of resident vascular cells between arterial beds, reflected by our transcriptomic analysis, is critical in understanding plaque biology and calcification, and may have strong implications in vascular therapeutic approaches. |
[Mh] Termos MeSH primário: |
Artérias/patologia Calcinose/patologia Músculo Liso Vascular/patologia
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[Mh] Termos MeSH secundário: |
Diferenciação Celular Células Cultivadas Seres Humanos Placa Aterosclerótica/patologia Transcriptoma
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180309 |
[Lr] Data última revisão:
| 180309 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 180127 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0191976 |
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