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Pesquisa : G04.152.650.249 [Categoria DeCS]
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[PMID]:29358748
[Au] Autor:Kucherenko MM; Shcherbata HR
[Ad] Endereço:Max Planck Research Group of Gene Expression and Signaling, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 11, Goettingen, 37077, Germany.
[Ti] Título:Stress-dependent miR-980 regulation of Rbfox1/A2bp1 promotes ribonucleoprotein granule formation and cell survival.
[So] Source:Nat Commun;9(1):312, 2018 01 22.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Upon stress, profound post-transcriptional adjustments of gene expression occur in spatially restricted, subcellular, membraneless compartments, or ribonucleoprotein (RNP) granules, which are formed by liquid phase separation of RNA-binding proteins with low complexity sequence domains (LCDs). Here, we show that Rbfox1 is an LCD-containing protein that aggregates into liquid droplets and amyloid-like fibers and promiscuously joins different nuclear and cytoplasmic RNP granules. Using Drosophila oogenesis as an in vivo system for stress response, we demonstrate a mechanism by which Rbfox1 promotes cell survival. The stress-dependent miRNA miR-980 acts to buffer Rbfox1 levels, since it targets only those Rbfox1 transcripts that contain extended 3'UTRs. Reduced miR-980 expression during stress leads to increased Rbfox1 levels, widespread formation of various RNP granules, and increased cell viability. We show that human RBFOX proteins also contain multiple LCDs and form membraneless compartments, suggesting that the RNP granule-linked control of cellular adaptive responses may contribute to a wide range of RBFOX-associated pathologies in humans.
[Mh] Termos MeSH primário: Proteínas de Drosophila/genética
MicroRNAs/genética
Oócitos/metabolismo
Proteínas de Ligação a RNA/genética
Ribonucleoproteínas/genética
Estresse Fisiológico/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Sobrevivência Celular
Reprogramação Celular
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/genética
Drosophila melanogaster/crescimento & desenvolvimento
Drosophila melanogaster/metabolismo
Feminino
Fibroblastos/citologia
Fibroblastos/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
MicroRNAs/metabolismo
Mutação
Neurônios/citologia
Neurônios/metabolismo
Oócitos/citologia
Oogênese/genética
Ovário/citologia
Ovário/metabolismo
Cultura Primária de Células
Domínios Proteicos
Proteínas de Ligação a RNA/metabolismo
Ribonucleoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (MicroRNAs); 0 (RNA-Binding Proteins); 0 (Rbfox1 protein, Drosophila); 0 (Ribonucleoproteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02757-w


  2 / 5506 MEDLINE  
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[PMID]:29292088
[Au] Autor:Yang LL; Zhao Y; Luo SM; Ma JY; Ge ZJ; Shen W; Yin S
[Ad] Endereço:College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
[Ti] Título:Toxic effects and possible mechanisms of hydrogen sulfide and/or ammonia on porcine oocyte maturation in vitro.
[So] Source:Toxicol Lett;285:20-26, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Previous studies suggest that hydrogen sulfide (H S) and ammonia (NH ) are two major air pollutants which can cause damage to porcine health. However, the mechanisms underlying toxic effects of these compounds on porcine oocyte maturation are not clear. To clarify the mechanism, we evaluated the oocyte quality by detecting some events during oocytes maturation. In our study, porcine oocytes were cultured with different concentrations of Na S and/or NH Cl in vitro and the rate of the first polar body extrusion decreased significantly. Also, actin filament was seriously disrupted to damage the cytoskeleton which resulted in reduced rate of oocyte maturation. We explored the reactive oxygen species (ROS) generation and found that the ROS level was increased significantly after Na S treatment but not after NH Cl treatment. Moreover, early stage apoptosis rate was significantly increased and autophagy protein LC3 B expression level was higher in oocytes treated with Na S and/or NH Cl, which might be caused by ROS elevation. Additionally, exposure to Na S and/or NH Cl also caused ROS generation and early apoptosis in cumulus cells, which might further affect oocyte maturation in vitro. In summary, our data suggested that exposure to H S and/or NH decreased porcine oocyte maturation in vitro, which might be caused by actin disruption, ROS generation, early apoptosis and autophagy.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/toxicidade
Amônia/toxicidade
Sulfeto de Hidrogênio/toxicidade
Oócitos/efeitos dos fármacos
Oogênese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Actinas/metabolismo
Amônia/administração & dosagem
Animais
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Células Cultivadas
Feminino
Sulfeto de Hidrogênio/administração & dosagem
Oócitos/metabolismo
Oócitos/patologia
Corpos Polares/efeitos dos fármacos
Corpos Polares/metabolismo
Corpos Polares/patologia
Espécies Reativas de Oxigênio/metabolismo
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Air Pollutants); 0 (Reactive Oxygen Species); 7664-41-7 (Ammonia); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


  3 / 5506 MEDLINE  
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[PMID]:29272351
[Au] Autor:Crowder CM; Lassiter CS; Gorelick DA
[Ad] Endereço:Department of Pharmacology & Toxicology, University of Alabama at Birmingham, Birmingham, Alabama.
[Ti] Título:Nuclear Androgen Receptor Regulates Testes Organization and Oocyte Maturation in Zebrafish.
[So] Source:Endocrinology;159(2):980-993, 2018 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Androgens act through the nuclear androgen receptor (AR) to regulate gonad differentiation and development. In mice, AR is necessary for spermatogenesis, testis development, and formation of external genitalia in males and oocyte maturation in females. However, the extent to which these phenotypes are conserved in nonmammalian vertebrates is not well understood. Here, we generate zebrafish with a mutation in the ar gene (aruab105/105) and examine the role of AR in sexual determination and gonad development. We found that zebrafish AR regulates male sexual determination, because the majority of aruab105/105 mutant embryos developed ovaries and displayed female secondary sexual characteristics. The small percentage of mutants that developed testes displayed female secondary sexual characteristics, exhibited structurally disorganized testes, and were unable to release or produce normal levels of sperm, demonstrating that AR is necessary for zebrafish testis development and fertility. In females, we found that AR regulates oocyte maturation and fecundity. The aruab105/105 mutant females developed ovaries filled primarily with immature stage I oocytes and few mature stage III oocytes. Two genes whose expression is enriched in wild-type ovaries compared with testes (cyp19a1a, foxl2a) were upregulated in ar mutant testes, and two genes enriched in testes (amh, dmrt1) were upregulated in ar mutant ovaries. These findings demonstrate that AR regulates sexual determination, testis development, and oocyte maturation and suggest that AR regulates sexually dimorphic gene expression. The ar mutant we developed will be useful for modeling human endocrine function in zebrafish.
[Mh] Termos MeSH primário: Oogênese/genética
Receptores Androgênicos/fisiologia
Diferenciação Sexual/genética
Espermatogênese/genética
Testículo/citologia
Testículo/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Núcleo Celular/metabolismo
Embrião não Mamífero
Feminino
Fertilidade/genética
Masculino
Receptores Androgênicos/genética
Testículo/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/genética
Peixe-Zebra/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Receptors, Androgen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00617


  4 / 5506 MEDLINE  
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[PMID]:27771155
[Au] Autor:Sükür YE; Özmen B; Özdemir ED; Seval MM; Kalafat E; Sönmezer M; Berker B; Aytaç R; Atabekoglu CS
[Ad] Endereço:Department of Obstetrics and Gynecology, Ankara University School of Medicine, Kadin Hastaliklari ve Dogum AD, 06100 Cebeci, Ankara, Turkey.
[Ti] Título:Final oocyte maturation with two different GnRH agonists in antagonist co-treated cycles at risk of ovarian hyperstimulation syndrome.
[So] Source:Reprod Biomed Online;34(1):5-10, 2017 Jan.
[Is] ISSN:1472-6491
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Triptorelin 0.2 mg and leuprolide 1 mg subcutaneous injections for triggering final follicular maturation were compared in patients with a high risk for ovarian hyperstimulation syndrome (OHSS). Infertile patients treated with GnRH antagonist protocol between January 2014 and March 2016 were recruited. Patients with high serum oestradiol levels on HCG day (>3000 pg/ml) indicating a risk of OHSS consisted of the study groups (A and B). Patients with serum oestradiol levels less than 3000 pg/ml consisted of the control group (C). A single injection of 0.2 mg triptorelin, 1 mg leuprolide and 10000 IU HCG were administered for final oocyte triggering in groups A (n = 63), B (n = 74) and C (n = 131), respectively. Demographic parameters were comparable between the groups. No cases of severe or moderate OHSS occurred in any group. The clinical pregnancy rates were 31.7%, 37.8% and 32.8% in groups A, B and C, respectively. Both injections had comparable efficacy in clinical outcome and OHSS risk. Regardless of preferred drug, GnRH agonist trigger for final oocyte maturation seems to be safe for patients with high OHSS risk, and can be safely used in fresh embryo transfer cycles.
[Mh] Termos MeSH primário: Hormônio Liberador de Gonadotropina/agonistas
Hormônio Liberador de Gonadotropina/antagonistas & inibidores
Oócitos/citologia
Síndrome de Hiperestimulação Ovariana/tratamento farmacológico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estradiol/sangue
Feminino
Antagonistas de Hormônios/uso terapêutico
Seres Humanos
Infertilidade Feminina/terapia
Infertilidade Masculina/terapia
Leuprolida/administração & dosagem
Masculino
Oócitos/efeitos dos fármacos
Oogênese
Indução da Ovulação
Gravidez
Taxa de Gravidez
Estudos Retrospectivos
Risco
Injeções de Esperma Intracitoplásmicas
Pamoato de Triptorrelina/administração & dosagem
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hormone Antagonists); 33515-09-2 (Gonadotropin-Releasing Hormone); 4TI98Z838E (Estradiol); 57773-63-4 (Triptorelin Pamoate); EFY6W0M8TG (Leuprolide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  5 / 5506 MEDLINE  
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[PMID]:29305975
[Au] Autor:Qiu W; Zhu Y; Wu Y; Yuan C; Chen K; Li M
[Ad] Endereço:Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; National Demonstrat
[Ti] Título:Identification and expression analysis of microRNAs in medaka gonads.
[So] Source:Gene;646:210-216, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gonad development is a highly regulated, coordinated biological process and increasing evidences have indicated that microRNA (miRNA) may be involved in this dynamic program. Medaka (Oryzias latipes) is a good model for reproductive research as it has distinct sex determining genes, however, research in gonadal miRNAs is lacked. In this study, two small RNA libraries from the ovaries and testes were constructed and sequenced. A total of 285 conserved and 388 novel miRNAs were obtained, among which 142 mature miRNAs were significantly (> two-fold change) up or down regulated in the testis compared to the ovary. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis showed that miR-430c, miR-26a and miR-202-5p were expressed in a gonad-specific or sex-biased pattern. Fluorescence in situ hybridization (FISH) indicated that miR-202-5p was present throughout spermatogenesis and was only detected at the early stages of oogenesis, this sex biased expression pattern suggested that miR-202-5p might be a crucial candidate in male differentiation and development. Our study provides the repertoire, a comprehensive annotation of miRNAs from gonads and a reference for functional studies of miRNAs in medaka.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
Gônadas/crescimento & desenvolvimento
MicroRNAs/genética
Oryzias/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Biblioteca Gênica
Gônadas/química
Hibridização in Situ Fluorescente
Masculino
Oogênese
Especificidade de Órgãos
Oryzias/genética
Sexismo
Espermatogênese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  6 / 5506 MEDLINE  
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[PMID]:28743001
[Au] Autor:Pae J; Cinalli RM; Marzio A; Pagano M; Lehmann R
[Ad] Endereço:HHMI and Kimmel Center for Biology and Medicine of the Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
[Ti] Título:GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.
[So] Source:Dev Cell;42(2):130-142.e7, 2017 07 24.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The separation of germline from somatic lineages is fundamental to reproduction and species preservation. Here, we show that Drosophila Germ cell-less (GCL) is a critical component in this process by acting as a switch that turns off a somatic lineage pathway. GCL, a conserved BTB (Broad-complex, Tramtrack, and Bric-a-brac) protein, is a substrate-specific adaptor for Cullin3-RING ubiquitin ligase complex (CRL3 ). We show that CRL3 promotes PGC fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate. This mode of RTK degradation does not depend upon receptor activation but is prompted by release of GCL from the nuclear envelope during mitosis. The cell-cycle-dependent change in GCL localization provides spatiotemporal specificity for RTK degradation and sequesters CRL3 to prevent it from participating in excessive activities. This precisely orchestrated mechanism of CRL3 function and regulation defines cell fate at the single-cell level.
[Mh] Termos MeSH primário: Linhagem da Célula
Proteínas Culina/metabolismo
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/citologia
Drosophila melanogaster/metabolismo
Proteínas Nucleares/metabolismo
Proteólise
Receptores Proteína Tirosina Quinases/metabolismo
[Mh] Termos MeSH secundário: Animais
Membrana Celular/metabolismo
Sequência Conservada
Proteínas de Drosophila/química
Células Germinativas/citologia
Células Germinativas/metabolismo
Células HEK293
Seres Humanos
Mitose
Membrana Nuclear/metabolismo
Sinais de Localização Nuclear/metabolismo
Proteínas Nucleares/química
Oogênese
Domínios Proteicos
Transdução de Sinais
Especificidade por Substrato
Ubiquitinação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cullin Proteins); 0 (Drosophila Proteins); 0 (Nuclear Localization Signals); 0 (Nuclear Proteins); 0 (gcl protein, Drosophila); 0 (gft protein, Drosophila); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.10.1 (torso protein, Drosophila)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171208
[Lr] Data última revisão:
171208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  7 / 5506 MEDLINE  
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[PMID]:28976982
[Au] Autor:Maenohara S; Unoki M; Toh H; Ohishi H; Sharif J; Koseki H; Sasaki H
[Ad] Endereço:Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
[Ti] Título:Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos.
[So] Source:PLoS Genet;13(10):e1007042, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The methylation of cytosine at CG sites in the mammalian genome is dynamically reprogrammed during gametogenesis and preimplantation development. It was previously shown that oocyte-derived DNMT1 (a maintenance methyltransferase) is essential for maintaining and propagating CG methylation at imprinting control regions in preimplantation embryos. In mammalian somatic cells, hemimethylated-CG-binding protein UHRF1 plays a critical role in maintaining CG methylation by recruiting DNMT1 to hemimethylated CG sites. However, the role of UHRF1 in oogenesis and preimplantation development is unknown. In the present study, we show that UHRF1 is mainly, but not exclusively, localized in the cytoplasm of oocytes and preimplantation embryos. However, smaller amounts of UHRF1 existed in the nucleus, consistent with the expected role in DNA methylation. We then generated oocyte-specific Uhrf1 knockout (KO) mice and found that, although oogenesis was itself unaffected, a large proportion of the embryos derived from the KO oocytes died before reaching the blastocyst stage (a maternal effect). Whole genome bisulfite sequencing revealed that blastocysts derived from KO oocytes have a greatly reduced level of CG methylation, suggesting that maternal UHRF1 is essential for maintaining CG methylation, particularly at the imprinting control regions, in preimplantation embryos. Surprisingly, UHRF1 was also found to contribute to de novo CG and non-CG methylation during oocyte growth: in Uhrf1 KO oocytes, transcriptionally-inactive regions gained less methylation, while actively transcribed regions, including the imprinting control regions, were unaffected or only slightly affected. We also found that de novo methylation was defective during the late stage of oocyte growth. To the best of our knowledge, this is the first study to demonstrate the role of UHRF1 in de novo DNA methylation in vivo. Our study reveals multiple functions of UHRF1 during the global epigenetic reprogramming of oocytes and early embryos.
[Mh] Termos MeSH primário: Blastocisto/metabolismo
Metilação de DNA
Proteínas Nucleares/metabolismo
Oócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Reprogramação Celular
DNA (Citosina-5-)-Metiltransferase 1
DNA (Citosina-5-)-Metiltransferases/genética
DNA (Citosina-5-)-Metiltransferases/metabolismo
Desenvolvimento Embrionário
Epigênese Genética
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Proteínas Nucleares/deficiência
Proteínas Nucleares/genética
Oócitos/crescimento & desenvolvimento
Oogênese
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nuclear Proteins); 0 (RNA, Messenger); 0 (Uhrf1 protein, mouse); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases); EC 2.1.1.37 (Dnmt1 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007042


  8 / 5506 MEDLINE  
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[PMID]:28942449
[Au] Autor:Wei S; Shen X; Gong Z; Deng Y; Lai L; Liang H
[Ad] Endereço:College of Life Science and Engineering, Northwest Minzu University, Lanzhou, China.
[Ti] Título:FSHR and LHR Expression and Signaling as Well as Maturation and Apoptosis of Cumulus-Oocyte Complexes Following Treatment with FSH Receptor Binding Inhibitor in Sheep.
[So] Source:Cell Physiol Biochem;43(2):660-669, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Currently, it remains unknown whether FSH receptor binding inhibitor (FRBI) influences follicular development and reproduction functions in humans and animals. The present study aimed to investigate FRBI effects on in vitro maturation (IVM) and apoptosis of cumulus-oocyte complexes (COCs) of sheep, to determine the effect of FRBI on mRNA and protein levels of FSHR and LHR in COCs, and to elucidate the signal pathway of FRBI effects. METHODS: COCs were in vitro cultured for 24h in the IVM media supplemented with varying concentrations of FRBI (0, 10, 20, 30 and 40µg/mL) and FSH (10IU/mL). The harvested COCs were observed under an inverted microscope and maturation rates of COCs were determined. Real time RT-PCR and Western blotting were utilized to detect mRNA and protein levels of FSHR and LHR. The concentrations of FSH, LH and caspase-3 were determined using especial ELISA kits for sheep, respectively. RESULTS: Maturation rates of COCs decreased gradually as FRBI concentrations increased from 0 to 40µg/mL, reaching a bottom value of 23.76% of the FRBI-4 group. The maximal apoptosis rate was detected in the FRBI-4 group. IP3 contents of FRBI-3 and FRBI-4 groups were reduced as compared to control group (CG) and FSH groups (P<0.05). Levels of FSHR protein of FRBI-3 and FRBI-4 groups as well as LHR protein of FRBI-4 group were significantly less than that of CG and FSH group. FSH contents of four FRBI treatment groups were gradually decreased along with the supplementation doses of FRBI. Caspase-3 contents of FRBI groups were reduced with a maximum reduction of the FRBI-2 group. CONCLUSION: Our results revealed supplement of FRBI into IVM media could dose-dependently decrease the maturation rate and increase apoptosis rate of sheep COCs. A lower dose of FRBI treatment slightly promoted IP3 production, but a higher dose of FRBI reduced IP3 production. FRBI suppressed the mRNA and protein expression levels of FSHR and LHR in sheep COCs. Our study will help to therapy effectively ovarian diseases, improve ovarian and follicular functions, and further to promote fertility of humans and animals.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Proteínas de Transporte/farmacologia
Células do Cúmulo/efeitos dos fármacos
Técnicas de Maturação in Vitro de Oócitos
Oócitos/efeitos dos fármacos
Fragmentos de Peptídeos/farmacologia
Receptores do FSH/genética
Receptores do LH/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Células do Cúmulo/citologia
Feminino
Regulação da Expressão Gênica/efeitos dos fármacos
Técnicas de Maturação in Vitro de Oócitos/métodos
Oócitos/citologia
Oogênese/efeitos dos fármacos
Ovinos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Peptide Fragments); 0 (Receptors, FSH); 0 (Receptors, LH); 0 (alanyl-glutamyl-seryl-asparagyl-glutamyl-aspartyl-glycyl-tyrosine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE
[do] DOI:10.1159/000480650


  9 / 5506 MEDLINE  
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[PMID]:28939612
[Au] Autor:O'Donnell MA
[Ti] Título:Margot Quinlan: Muscling in on oogenesis.
[So] Source:J Cell Biol;216(10):2992-2993, 2017 Oct 02.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quinlan investigates how the cytoskeleton polarizes oocytes.
[Mh] Termos MeSH primário: Citoesqueleto/metabolismo
Miosinas/metabolismo
Oogênese/fisiologia
[Mh] Termos MeSH secundário: Animais
Citoesqueleto/genética
Feminino
História do Século XX
História do Século XXI
Seres Humanos
Miosinas/genética
Miosinas/história
Retratos como Assunto
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201709035


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Texto completo
[PMID]:28938096
[Au] Autor:Zhao BS; He C
[Ad] Endereço:Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, 929 East 57th Street, The University of Chicago, Chicago, Illinois 60637, USA.
[Ti] Título:"Gamete On" for m A: YTHDF2 Exerts Essential Functions in Female Fertility.
[So] Source:Mol Cell;67(6):903-905, 2017 Sep 21.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this issue of Molecular Cell, Ivanova et al. (2017) report key functions of the m A reader YTHDF2 in the regulation of mammalian development during oocyte maturation and early zygotic development.
[Mh] Termos MeSH primário: Células Germinativas
Zigoto
[Mh] Termos MeSH secundário: Animais
Feminino
Fertilidade
Seres Humanos
Oócitos
Oogênese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE



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