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Pesquisa : G04.152.650.624.700 [Categoria DeCS]
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[PMID]:28358879
[Au] Autor:Bundgaard J; Barker JS
[Ad] Endereço:Section for Genetics, Ecology and Evolution, Department of Bioscience, Aarhus University, Ny Munkegade 116, Aarhus, Denmark.
[Ti] Título:Genetic variation for resistance to high temperature stress of mature sperm - a study in Drosophila.
[So] Source:PLoS One;12(3):e0173990, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genetic variation for resistance to heat stress has been found for a number of life-history components in Drosophila species. For male and female fertility (or sterility), stress resistance of the parents is confounded with stress resistance of the haploid gametes. Many genes are known to influence male fertility in Drosophila melanogaster. Some may carry temperature sensitive alleles that reduce fertility through effects on mature sperm when exposed to heat stress. In this study, sperm from each of 320 males were either not heat shocked (control) or exposed to a heat shock (36.9°C for 2 hours) either in the male testes or in the female reproductive tract. We did not detect any temperature sensitive sterility alleles. These results are relevant in relation to haploid gene expression and the findings of considerable amounts of mRNA in mature sperm, potentially important for sperm function and fertilization.
[Mh] Termos MeSH primário: Drosophila melanogaster/fisiologia
Variação Genética
Resposta ao Choque Térmico/genética
Maturação do Esperma/genética
[Mh] Termos MeSH secundário: Alelos
Animais
Drosophila melanogaster/genética
Feminino
Fertilização/genética
Regulação da Expressão Gênica no Desenvolvimento/genética
Temperatura Alta
Masculino
RNA Mensageiro/biossíntese
Testículo/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173990


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[PMID]:28296254
[Au] Autor:Casamonti E; Vinci S; Serra E; Fino MG; Brilli S; Lotti F; Maggi M; Coccia ME; Forti G; Krausz C
[Ad] Endereço:Sexual Medicine and Andrology Unit, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Florence, Italy.
[Ti] Título:Short-term FSH treatment and sperm maturation: a prospective study in idiopathic infertile men.
[So] Source:Andrology;5(3):414-422, 2017 May.
[Is] ISSN:2047-2927
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The standard FSH treatment is based on a 3 months period, after which both quantitative/qualitative improvement of sperm parameters and increased pregnancy rate were reported. In this prospective clinical trial, for the first time, we studied (i) Sperm hyaluronic acid binding capacity after highly purified FSH (hpFSH) treatment; (ii) the effect after short-term and standard treatment on this functional parameter. As secondary objective, we analyzed three SNPs on FSHß and FSHR genes to define their potential predictive value for responsiveness. From a total of 210 consecutive patients, 40 oligo- and/or astheno- and/or teratozoospermic patients fulfilled the inclusion criteria. Treatment consisted in hpFSH 75 IU/L every other day for 3 months. To avoid potential biases derived from the lack of placebo, we analyzed each patient after 4-6 months of 'wash-out' period. After FSH treatment, we observed a statistically significant (p < 0.001) improvement of the percentage of hyaluronic acid bound spermatozoa from basal to T1 (after 1 month) and to T3 (after 3 months). Importantly, these values returned to near-baseline value after the wash-out. The same results were detected for total motile sperm count after 3 months with return to baseline after wash-out. Forty-two percent of patients responded to the therapy with increasing hyaluronic acid binding capacity above the double of the Intraindividual Variation (IV) while 24% of patients reached above the normal Sperm-Hyaluronan Binding Assay (HBA) value. Further increase in 'responders' was observed at T3. The responsiveness to treatment resulted independent from FSHR/FSHß polymorphisms. The significant positive effect on sperm maturity after 1 month opens novel therapeutic perspectives. In view of both the high cost and the relative invasiveness of treatment, the short protocol (1 month) could represent a viable FSH treatment option prior Assisted Reproductive Techniques since FSH, by acting on sperm maturation, increases the proportion of functionally competent cells.
[Mh] Termos MeSH primário: Hormônio Foliculoestimulante/uso terapêutico
Infertilidade Masculina/tratamento farmacológico
Maturação do Esperma/efeitos dos fármacos
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Proteínas de Transporte/genética
Genótipo
Glicopeptídeos/genética
Seres Humanos
Infertilidade Masculina/genética
Masculino
Polimorfismo de Nucleotídeo Único
Estudos Prospectivos
Receptores do FSH/genética
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (FSH-BI protein, human); 0 (Glycopeptides); 0 (Receptors, FSH); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1111/andr.12333


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[PMID]:28126553
[Au] Autor:Kopalli SR; Cha KM; Ryu JH; Lee SH; Jeong MS; Hwang SY; Lee YJ; Song HW; Kim SN; Kim JC; Kim SK
[Ad] Endereço:Department of Biomedical Chemistry, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Republic of Korea.
[Ti] Título:Korean red ginseng improves testicular ineffectiveness in aging rats by modulating spermatogenesis-related molecules.
[So] Source:Exp Gerontol;90:26-33, 2017 Apr.
[Is] ISSN:1873-6815
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Korean red ginseng (Panax ginseng Meyer) is known to rejuvenate testicular effectiveness and the sperm maturation process by regulating redox proteins in aged rats. This study was performed to investigate the effect of Korean red ginseng water extract (KRG-WE) on the expression level of spermatogenesis-related key biomolecules and sex hormone receptors as well as enzymes regulating oxidation, histone deacetylation, and growth-related activities in aged rat testis. KRG-WE (200mg/kg) mixed with a regular pellet diet was administered to 12-month-old rats for 6months (KRG-AC), whereas the young (YC, 2months) and aged (AC, 12months) controls received the vehicle only. The results showed that the expression levels of spermatogenesis-related key biomolecules (inhibin-α, nectin-2, and cyclic adenosine monophosphate [cAMP] responsive element binding protein [CREB]-1), sex hormone receptors (androgen, luteinizing- and follicle-stimulating hormone receptors [AR, LHR, and FSHR, respectively]), and antioxidant enzymes (glutathione S-transferase mu [GSTm]-5, glutathione peroxidase [GPx]-4, peroxiredoxin [PRx]-3), as well as histone deactylation (silent mating type information regulation 2 homolog 1, SIRT1) and growth-related (mammalian target of rapamycin complex 1, mTORC1) molecules were significantly altered in the AC group rat testes compared with those of the YC group. However, KRG-WE treatment of the AC group significantly (p<0.05) attenuated these molecular changes. From these results, it can be concluded that long-term administration of KRG-WE significantly delayed the aging-induced testicular dysfunction.
[Mh] Termos MeSH primário: Envelhecimento/metabolismo
Antioxidantes/farmacologia
Panax
Extratos Vegetais/farmacologia
Maturação do Esperma/efeitos dos fármacos
Espermatozoides/metabolismo
[Mh] Termos MeSH secundário: Envelhecimento/efeitos dos fármacos
Animais
Masculino
Oxirredução
Fitoterapia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


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[PMID]:28093975
[Au] Autor:Sarma K; Roychoudhury S; Bora SS; Dehury B; Parida P; Das S; Das R; Dohutia C; Nath S; Deb B; Modi MK
[Ad] Endereço:Department of Life Science and Bioinformatics, Assam University, Silchar 788011, Assam. India.
[Ti] Título:Molecular Modeling and Dynamics Simulation Analysis of KATNAL1 for Identification of Novel Inhibitor of Sperm Maturation.
[So] Source:Comb Chem High Throughput Screen;20(1):82-92, 2017.
[Is] ISSN:1875-5402
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hormone based birth control often causes various side effects. A recent study revealed that temporary infertility without changing hormone levels can be attained by inhibiting Katanin p60 ATPase-containing subunit A-like 1 protein (KATNAL1) which is critical for sperm maturation in the testes. OBJECTIVE: This study aimed at attaining the most energetically stable three dimensional (3D) structure of KATNAL1 protein using comparative modeling followed by screening of a ligand library of known natural spermicidal compounds for their binding affinity with KATNAL1. This in turn may inhibit the development of mature sperm in the seminiferous epithelium. METHOD: A series of computational techniques were used for building the 3D structure of KATNAL1 which was further optimized by molecular dynamics (MD) simulation. For revealing the ATP binding mode of KATNAL1, docking study was carried out using the optimized model obtained from the MD simulation. The docking study was also employed to test the binding efficiency of the ligand library. RESULTS: Molecular docking study confirmed the ATP binding of KATNAL1 with various hydrophobic and hydrogen bond interactions. Binding efficiency of the ligand library suggested that calotropin, a cardenolide of Calotropis procera showed the highest binding efficiency against the target protein without toxicity. MD simulation of the docked complex validated the results of the docking study. CONCLUSION: This study revealed the ATP binding mode of KATNAL1 and identified calotropin as a potential lead molecule against it showing high binding efficiency with good bioavailability and no mutagenicity. Further in vitro and in vivo bioassay of calotropin could facilitate the development of novel non-hormonal male-specific contraceptive in near future.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/antagonistas & inibidores
Adenosina Trifosfatases/metabolismo
Anticoncepcionais Masculinos/farmacologia
Descoberta de Drogas
Maturação do Esperma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/química
Trifosfato de Adenosina/metabolismo
Sítios de Ligação
Cardenolídeos/farmacologia
Seres Humanos
Katanina
Ligantes
Masculino
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Ligação Proteica
Relação Quantitativa Estrutura-Atividade
Bibliotecas de Moléculas Pequenas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cardenolides); 0 (Contraceptive Agents, Male); 0 (Ligands); 0 (Small Molecule Libraries); 0 (calotropin); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.4.3 (KATNAL1 protein, human); EC 3.6.4.3 (Katanin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE
[do] DOI:10.2174/1386207320666170116120104


  5 / 899 MEDLINE  
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[PMID]:27862401
[Au] Autor:Majewska AM; Kordan W; Koziorowska-Gilun M; Wysocki P
[Ad] Endereço:Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
[Ti] Título:Identification and changes in the seasonal concentrations of heat shock proteins in roe deer (Capreolus capreolus) epididymides.
[So] Source:Reprod Domest Anim;52(1):107-114, 2017 Feb.
[Is] ISSN:1439-0531
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Heat shock proteins (HSPs) act as molecular chaperones with important regulatory functions. HSPs are considered to be essential factors in animal reproduction. In view of seasonal variations in the secretory activity of the reproductive tract of mature roe deer (Capreolus capreolus), the aims of this study were to identify HSPs in the epididymides and compare the expression of the identified proteins in three periods of the reproductive season. Two-dimensional polyacrylamide gel electrophoresis revealed the highest number of polypeptides in homogenates of epididymal tissues and in caput, corpus and cauda epididymal fluids throughout the reproductive season. Epididymal tissue homogenates and epididymal fluids were analysed by tandem mass spectrometry (MS/MS) to reveal 31 polypeptides with enzymatic activity, including polypeptides with antioxidant properties, structural and cell signalling functions. Moreover, among the identified polypeptides, five of them were similar to heat shock proteins: endoplasmin (Grp94); heat shock protein 90 kDa (HSP90); 78-kDa glucose-regulated protein (Grp78); chain A, the crystal structure of the human HSP70 ATPase domain and heat shock protein beta-1 isoform X. The concentrations of the analysed polypeptides, expressed in optical density units (ODU), differed significantly (p ≤ .05) across the examined periods of the reproductive season. The highest ODU values for almost all analysed proteins were observed during the rutting period. The presence of HSPs in the epididymal tissues and fluids of roe deer in different periods of the reproductive season could indicate that those proteins play an important role in sperm maturation in the epididymis.
[Mh] Termos MeSH primário: Cervos
Epididimo/metabolismo
Proteínas de Choque Térmico/metabolismo
Estações do Ano
Maturação do Esperma
[Mh] Termos MeSH secundário: Animais
Eletroforese em Gel Bidimensional
Epididimo/citologia
Seres Humanos
Masculino
Espermatozoides/citologia
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heat-Shock Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/rda.12816


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[PMID]:27807895
[Au] Autor:Rowlison T; Ottinger MA; Comizzoli P
[Ad] Endereço:Smithsonian Conservation Biology Institute, Washington, DC, USA.
[Ti] Título:Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat.
[So] Source:Reprod Domest Anim;52 Suppl 2:193-196, 2017 Apr.
[Is] ISSN:1439-0531
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The sperm centrosome is an essential organelle with a key role in organizing the sperm aster for proper syngamy and formation of the first mitotic spindle. The sperm cell acquires the functional capability during epididymal transit by incorporation of key factors. The objective of the study was to identify these key maturation proteins, such as ninein and centriolin as well as cenexin-a scaffold protein that serves to bind ninein and centriolin. Epididymal samples were dissected from 17 adult cat testes (>1 year old) and spermatozoa were extracted from the different regions, including rete testis, caput, corpus, cauda and vas deferens. Tissue samples and sperm cells were fixed separately in 4% paraformaldehyde before immunostaining with anticenexin, ninein or centriolin antibodies. Results showed that the proportion of sperm cells with cenexin localized at the centrosome progressively increased along the tract with the lowest percentage of stained cells in the testis (mean = 45%) and highest in the cauda (mean = 81%). Although not significant, the intensity of cenexin immunofluorescence in positive cells increased twofold from the testis to vas deferens. There was no significant difference in the proportion of sperm labelled with centriolin or ninein (ranges of 21%-26% and 33%-48% between segments, respectively) or the intensity (±58% and ±63% change as compared to testis, respectively). Cenexin may serve as a scaffold protein for centriolin and ninein, as the vast majority of spermatozoa only displayed colocalization of these proteins when cenexin was also present (mean = 85% and 91% colocalization, respectively). In summary, these results could be applied to future efforts to create an in vitro culture system capable of rescuing the impaired centrosome of an infertile male, with particular potential for wild felid conservation.
[Mh] Termos MeSH primário: Gatos
Proteínas de Ciclo Celular/fisiologia
Proteínas do Citoesqueleto/fisiologia
Proteínas de Choque Térmico/fisiologia
Maturação do Esperma/fisiologia
Transporte Espermático/fisiologia
[Mh] Termos MeSH secundário: Animais
Epididimo/citologia
Masculino
Espermatozoides/fisiologia
Testículo/citologia
Ducto Deferente/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Cytoskeletal Proteins); 0 (Heat-Shock Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE
[do] DOI:10.1111/rda.12831


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[PMID]:27655389
[Au] Autor:Caroppo E; Colpi EM; Gazzano G; Vaccalluzzo L; Scroppo FI; D'Amato G; Colpi GM
[Ad] Endereço:Reproductive Unit and IVF Center, ASL Bari, PTA F Jaia, via de Amicis 20, 70014, Conversano, BA, Italy. ecaroppo@teseo.it.
[Ti] Título:Testicular histology may predict the successful sperm retrieval in patients with non-obstructive azoospermia undergoing conventional TESE: a diagnostic accuracy study.
[So] Source:J Assist Reprod Genet;34(1):149-154, 2017 Jan.
[Is] ISSN:1573-7330
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The present study sought to determine the diagnostic accuracy of FSH level, testicular volume, and testicular histology in predicting the successful sperm retrieval (SSR) in a large cohort of patients with non-obstructive azoospermia undergoing conventional testicular sperm extraction (TESE). METHODS: We retrospectively evaluated 356 patients with non-obstructive azoospermia between June 2004 and July 2009. Binary logistic regression was used to evaluate the diagnostic accuracy of our predicting model, identifying sperm retrieval rate as binary dependent variable. The predictive accuracy of all variables individually evaluated was quantified with area under curve (AUC) estimates derived from receiver operating characteristic (ROC) curve. RESULTS: The mean patients' age was 36.8 years. Testicular sperm were retrieved in 158 out of 356 patients (44.3 %). Histological diagnosis of Sertoli cell only syndrome (SCO) was obtained in 216 patients (60.6 %), while 55 patients (15.4 %) had maturation arrest (MA) and 85 (23.8 %) had hypospermatogenesis (HYPO). The binary logistic regression model was statistically significant (χ = 96.792, p < 0.0001) and correctly classified 72.8 % of cases with 46.8 % sensitivity and 93.4 % specificity, positive predictive value (PPV) 85.06 %, negative predictive value (NPV) 68.7 %, +likelihood ratio (LR) 7.13, and -LR 0.57. Only testicular histology was significant to the model, while FSH and testicular volume were not. Sperm retrieval rate (SRR) was significantly higher in patients with HYPO compared to patients with SCO or MA (88.2 vs 30.5 and 30.9 %, respectively, p < 0.0001) CONCLUSIONS: This study demonstrates that including testicular histology in a model for predicting sperm retrieval increases its diagnostic accuracy. As histology is not available prior to TESE, this model applies only to patients with previous testicular surgery.
[Mh] Termos MeSH primário: Azoospermia/diagnóstico
Oligospermia/diagnóstico
Síndrome de Células de Sertoli/diagnóstico
Recuperação Espermática
[Mh] Termos MeSH secundário: Adulto
Azoospermia/patologia
Seres Humanos
Masculino
Meia-Idade
Oligospermia/patologia
Síndrome de Células de Sertoli/patologia
Maturação do Esperma
Espermatozoides/patologia
Testículo/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160923
[St] Status:MEDLINE
[do] DOI:10.1007/s10815-016-0812-3


  8 / 899 MEDLINE  
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[PMID]:27292792
[Au] Autor:Judycka S; Ciereszko A; Dobosz S; Zalewski T; Dietrich GJ
[Ad] Endereço:Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
[Ti] Título:Effect of dilution in sperm maturation media and time of storage on sperm motility and fertilizing capacity of cryopreserved semen of sex-reversed female rainbow trout.
[So] Source:Gen Comp Endocrinol;245:89-93, 2017 May 01.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Masculinized females, also called neomales or sex-reversed females have a male phenotype but retain the female genotype (XX). Therefore, all spermatozoa produced in their functional testes carry an X chromosome, which is desired for the production of all-female rainbow trout populations. Semen of sex-reversed female rainbow trout is of low quality and in vitro maturation is required, which includes dilution of sperm suspensions with specially formulated maturation solutions. The aim of this study was to determine the effect of dilution in different maturation media on sperm quality (sperm motility characteristics and fertilizing capacity) of frozen/thawed sperm of sex-reversed female rainbow trout. The effect of time of post-thaw storage (0, 15, 60 and 120min) on semen quality was also tested. Sperm motility parameters and fertilization rate at the eyed and hatching stages were assessed for post-thaw semen diluted in different media. The cryopreservation procedure resulted in high post-thaw sperm motility of about 57% and did not differ from fresh semen. Unexpectedly, maturation media decreased sperm activation capacity immediately after dilution; however, sperm motility increased over time. Fertilization rates of frozen/thawed semen were high (71-87%) and did not differ significantly between experimental variants at any of tested periods of storage. Our results demonstrated that the effect of the maturation media on frozen/thawed sperm is different from that of fresh sperm. The progressive increase in post-thaw sperm motility in maturation media can potentially be applied to routine hatchery practice.
[Mh] Termos MeSH primário: Criopreservação/veterinária
Análise do Sêmen/veterinária
Sêmen/fisiologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Feminino
Fertilização
Fertilização In Vitro/veterinária
Congelamento
Masculino
Oncorhynchus mykiss/fisiologia
Técnicas Reprodutivas/veterinária
Preservação do Sêmen
Maturação do Esperma
Motilidade Espermática/fisiologia
Espermatozoides/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE


  9 / 899 MEDLINE  
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[PMID]:27959275
[Au] Autor:Halder T; Datta U; Basu S; Mukherjee P
[Ad] Endereço:Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37 and 68, K.B. Sarani, Belgachia, Kolkata 700037, West Bengal, India. Electronic address: drtanmoyvet86@gmail.com.
[Ti] Título:Is lithium essential for epididymal sperm maturation?
[So] Source:Med Hypotheses;96:48-50, 2016 Nov.
[Is] ISSN:1532-2777
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A wider biological role of ultratrace element lithium in the mammalian reproduction has been reported, however, presence of lithium in the epididymal luminal fluid (ELF) and its influence on sperm during maturation events in the epididymal regions are still unknown. A pilot study was carried out in Jamunapari buck which revealed that levels of lithium in the ELF diminished gradually and significantly (P<0.01) from caput to cauda epididymis, concomitantly, a distinct increase (P<0.01) in the spermatozoan motility, viability and hypo-osmotic reactive sperm were observed, except spermatozoan motility that was found absent in the caput epididymis. Therefore, we hypothesize that levels of lithium in the epididymal regions is one of the motility initiation and/or regulatory factor for epididymal sperm maturation essential for acquiring fertilizing competence of sperm cells, hence, lithium could also be considered as one of the biomarker of sperm maturation in any species.
[Mh] Termos MeSH primário: Epididimo/efeitos dos fármacos
Lítio/química
Maturação do Esperma/efeitos dos fármacos
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Cátions
Homeostase
Seres Humanos
Masculino
Modelos Teóricos
Osmose
Projetos Piloto
Espermatozoides/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cations); 9FN79X2M3F (Lithium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE


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[PMID]:27695046
[Au] Autor:Jerczynski O; Lacroix-Pépin N; Boilard E; Calvo E; Bernet A; Fortier MA; Björkgren I; Sipilä P; Belleannée C
[Ad] Endereço:Department of Obstetrics, Gynecology and Reproduction, Université Laval, CHU de Québec Research Center (CHUL), Quebec City, Quebec, Canada.
[Ti] Título:Role of Dicer1-Dependent Factors in the Paracrine Regulation of Epididymal Gene Expression.
[So] Source:PLoS One;11(10):e0163876, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dicer1 is an endoribonuclease involved in the biogenesis of functional molecules such as microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs). These small non-coding RNAs are important regulators of post-transcriptional gene expression and participate in the control of male fertility. With the knowledge that 1) Dicer1-dependent factors are required for proper sperm maturation in the epididymis, and that 2) miRNAs are potent mediators of intercellular communication in most biological systems, we investigated the role of Dicer1-dependent factors produced by the proximal epididymis (initial segment/caput)- including miRNAs- on the regulation of epididymal gene expression in the distal epididymis regions (i.e. corpus and cauda). To this end, we performed comparative microarray and ANOVA analyses on control vs. Defb41iCre/wt;Dicer1fl/fl mice in which functional Dicer1 is absent from the principal cells of the proximal epididymis. We identified 35 and 33 transcripts that displayed significant expression level changes in the corpus and cauda regions (Fold change > 2 or < -2; p < 0.002), respectively. Among these transcripts, Zn-alpha 2-glycoprotein (Azgp1) encodes for a sperm equatorial protein whose expression in the epididymis of Dicer1 cKO mice is significantly increased compared to controls. In addition, 154 miRNAs, including miR-210, miR-672, miR-191 and miR-204, showed significantly impaired biogenesis in the absence of Dicer1 from the principal cells of the proximal epididymis (Fold change > 2 or < -2; p < 0.01). These miRNAs are secreted via extracellular vesicles (EVs) derived from the DC2 epididymal principal cell line, and their expression correlates with target transcripts involved in distinct biological pathways, as evidenced by in silico analysis. Albeit correlative and based on in silico approach, our study proposes that Dicer1-dependent factors trigger- directly or not-significant genes expression changes in distinct regions of this organ. The paracrine control of functions important to post-testicular sperm maturation by Dicer1-dependent factors may open new avenues for the identification of molecular targets important to male fertility control.
[Mh] Termos MeSH primário: RNA Helicases DEAD-box/biossíntese
Epididimo/crescimento & desenvolvimento
Fertilidade/genética
MicroRNAs/biossíntese
Ribonuclease III/biossíntese
Maturação do Esperma/genética
[Mh] Termos MeSH secundário: Animais
RNA Helicases DEAD-box/genética
Epididimo/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Glicoproteínas/biossíntese
Glicoproteínas/genética
Masculino
Camundongos
MicroRNAs/genética
Ribonuclease III/genética
Espermatozoides/crescimento & desenvolvimento
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoproteins); 0 (MicroRNAs); EC 3.1.26.3 (Dicer1 protein, mouse); EC 3.1.26.3 (Ribonuclease III); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0163876



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