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Pesquisa : G04.400.095 [Categoria DeCS]
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  1 / 5191 MEDLINE  
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[PMID]:27777629
[Au] Autor:Lau AC; Zhu KP; Brouhard EA; Davis MB; Csankovszki G
[Ad] Endereço:Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 N. University Ave., Ann Arbor, MI 48109-1048 USA ; Genome Technologies, The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032 USA.
[Ti] Título:An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in males.
[So] Source:Epigenetics Chromatin;9:44, 2016.
[Is] ISSN:1756-8935
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In , in order to equalize gene expression between the sexes and balance X and autosomal expression, two steps are believed to be required. First, an unknown mechanism is hypothesized to upregulate the X chromosome in both sexes. This mechanism balances the X to autosomal expression in males, but creates X overexpression in hermaphrodites. Therefore, to restore the balance, hermaphrodites downregulate gene expression twofold on both X chromosomes. While many studies have focused on X chromosome downregulation, the mechanism of X upregulation is not known. RESULTS: To gain more insight into X upregulation, we studied the effects of chromatin condensation and histone acetylation on gene expression levels in male . We have found that the H4K16 histone acetyltransferase MYS-1/Tip60 mediates dramatic decondensation of the male X chromosome as measured by FISH. However, RNA-seq analysis revealed that MYS-1 contributes only slightly to upregulation of gene expression on the X chromosome. These results suggest that the level of chromosome decondensation does not necessarily correlate with the degree of gene expression change in vivo. Furthermore, the X chromosome is more sensitive to MYS-1-mediated decondensation than the autosomes, despite similar levels of H4K16ac on all chromosomes, as measured by ChIP-seq. H4K16ac levels weakly correlate with gene expression levels on both the X and the autosomes, but highly expressed genes on the X chromosome do not contain exceptionally high levels of H4K16ac. CONCLUSION: These results indicate that H4K16ac and chromosome decondensation influence regulation of the male X chromosome; however, they do not fully account for the high levels of gene expression observed on the X chromosomes.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/metabolismo
Histona Acetiltransferases/metabolismo
Cromossomo X/metabolismo
[Mh] Termos MeSH secundário: Acetilação
Animais
Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/genética
Cromatina/metabolismo
Montagem e Desmontagem da Cromatina
Imunoprecipitação da Cromatina
Compensação de Dosagem (Genética)
Expressão Gênica
Histona Acetiltransferases/genética
Histonas/metabolismo
Hibridização in Situ Fluorescente
Masculino
Análise de Sequência de DNA
Cromossomo X/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Chromatin); 0 (Histones); EC 2.3.1.48 (Histone Acetyltransferases); EC 2.3.1.48 (MYS-1 protein, C elegans)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180310
[Lr] Data última revisão:
180310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  2 / 5191 MEDLINE  
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[PMID]:29254924
[Au] Autor:Cui TT; Xing TY; Chu YK; Li H; Wang N
[Ad] Endereço:1. Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; 2. Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; 3. Key Laboratory of Animal Cells and Genetic Engineering of Heilon
[Ti] Título:Genetic and epigenetic regulation of PPARγ during adipogenesis.
[So] Source:Yi Chuan;39(11):1066-1077, 2017 Nov 20.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Peroxisome proliferator-activated receptor gamma (PPARγ) is the master regulator of adipogenesis and adipose tissue development. It also plays crucial roles in many other biological processes, including lipid and glucose metabolism and energy homeostasis. Recently, evidence has been accumulating that the PPARγ gene is not only genetically regulated, but also epigenetically regulated by DNA methylation, histone modification, non-coding RNA and chromosome remodeling. In this review, we summarize the advances in the genetic and epigenetic regulation of the PPARγ gene during adipogenesis, and discuss future research directions and trends for the study of its regulation.
[Mh] Termos MeSH primário: Adipogenia
Metilação de DNA
PPAR gama/genética
[Mh] Termos MeSH secundário: Animais
Montagem e Desmontagem da Cromatina
Histonas/metabolismo
Seres Humanos
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Histones); 0 (PPAR gamma)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.16288/j.yczz.17-121


  3 / 5191 MEDLINE  
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[PMID]:29352283
[Au] Autor:Contreras C; Villasana M; Hendzel MJ; Carrero G
[Ad] Endereço:Department of Mathematical and Statistical Sciences, University of Alberta, Edmonton, Alberta, Canada.
[Ti] Título:Using a model comparison approach to describe the assembly pathway for histone H1.
[So] Source:PLoS One;13(1):e0191562, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Histones H1 or linker histones are highly dynamic proteins that diffuse throughout the cell nucleus and associate with chromatin (DNA and associated proteins). This binding interaction of histone H1 with the chromatin is thought to regulate chromatin organization and DNA accessibility to transcription factors and has been proven to involve a kinetic process characterized by a population that associates weakly with chromatin and rapidly dissociates and another population that resides at a binding site for up to several minutes before dissociating. When considering differences between these two classes of interactions in a mathematical model for the purpose of describing and quantifying the dynamics of histone H1, it becomes apparent that there could be several assembly pathways that explain the kinetic data obtained in living cells. In this work, we model these different pathways using systems of reaction-diffusion equations and carry out a model comparison analysis using FRAP (fluorescence recovery after photobleaching) experimental data from different histone H1 variants to determine the most feasible mechanism to explain histone H1 binding to chromatin. The analysis favors four different chromatin assembly pathways for histone H1 which share common features and provide meaningful biological information on histone H1 dynamics. We show, using perturbation analysis, that the explicit consideration of high- and low-affinity associations of histone H1 with chromatin in the favored assembly pathways improves the interpretation of histone H1 experimental FRAP data. To illustrate the results, we use one of the favored models to assess the kinetic changes of histone H1 after core histone hyperacetylation, and conclude that this post-transcriptional modification does not affect significantly the transition of histone H1 from a weakly bound state to a tightly bound state.
[Mh] Termos MeSH primário: Montagem e Desmontagem da Cromatina/fisiologia
Histonas/metabolismo
Modelos Biológicos
[Mh] Termos MeSH secundário: Acetilação
Animais
Cromatina/química
Cromatina/metabolismo
DNA/metabolismo
Recuperação de Fluorescência Após Fotodegradação
Histonas/química
Cinética
Ligação Proteica
Processamento de Proteína Pós-Traducional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromatin); 0 (Histones); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191562


  4 / 5191 MEDLINE  
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[PMID]:29335486
[Au] Autor:Ramírez F; Bhardwaj V; Arrigoni L; Lam KC; Grüning BA; Villaveces J; Habermann B; Akhtar A; Manke T
[Ad] Endereço:Max Planck Institute of Immunobiology and Epigenetics, Stübeweg 51, 79108, Freiburg, Germany.
[Ti] Título:High-resolution TADs reveal DNA sequences underlying genome organization in flies.
[So] Source:Nat Commun;9(1):189, 2018 01 15.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Despite an abundance of new studies about topologically associating domains (TADs), the role of genetic information in TAD formation is still not fully understood. Here we use our software, HiCExplorer (hicexplorer.readthedocs.io) to annotate >2800 high-resolution (570 bp) TAD boundaries in Drosophila melanogaster. We identify eight DNA motifs enriched at boundaries, including a motif bound by the M1BP protein, and two new boundary motifs. In contrast to mammals, the CTCF motif is only enriched on a small fraction of boundaries flanking inactive chromatin while most active boundaries contain the motifs bound by the M1BP or Beaf-32 proteins. We demonstrate that boundaries can be accurately predicted using only the motif sequences at open chromatin sites. We propose that DNA sequence guides the genome architecture by allocation of boundary proteins in the genome. Finally, we present an interactive online database to access and explore the spatial organization of fly, mouse and human genomes, available at http://chorogenome.ie-freiburg.mpg.de .
[Mh] Termos MeSH primário: Cromatina/ultraestrutura
Mapeamento Cromossômico/métodos
Cromossomos de Insetos/ultraestrutura
Drosophila melanogaster/genética
Genoma de Inseto
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Fator de Ligação a CCCTC/genética
Fator de Ligação a CCCTC/metabolismo
Cromatina/química
Montagem e Desmontagem da Cromatina
Cromossomos de Insetos/química
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Bases de Dados Genéticas
Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/ultraestrutura
Proteínas do Olho/genética
Proteínas do Olho/metabolismo
Expressão Gênica
Seres Humanos
Camundongos
Conformação Molecular
Motivos de Nucleotídeos
Software
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (BEAF-32 protein, Drosophila); 0 (CCCTC-Binding Factor); 0 (CTCF protein, Drosophila); 0 (Chromatin); 0 (DNA-Binding Proteins); 0 (Drosophila Proteins); 0 (Eye Proteins); 0 (M1BP protein, Drosophila); 0 (Transcription Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02525-w


  5 / 5191 MEDLINE  
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[PMID]:29335463
[Au] Autor:Wang Q; Sun Q; Czajkowsky DM; Shao Z
[Ad] Endereço:Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 200240, Shanghai, China.
[Ti] Título:Sub-kb Hi-C in D. melanogaster reveals conserved characteristics of TADs between insect and mammalian cells.
[So] Source:Nat Commun;9(1):188, 2018 01 15.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Topologically associating domains (TADs) are fundamental elements of the eukaryotic genomic structure. However, recent studies suggest that the insulating complexes, CTCF/cohesin, present at TAD borders in mammals are absent from those in Drosophila melanogaster, raising the possibility that border elements are not conserved among metazoans. Using in situ Hi-C with sub-kb resolution, here we show that the D. melanogaster genome is almost completely partitioned into >4000 TADs, nearly sevenfold more than previously identified. The overwhelming majority of these TADs are demarcated by the insulator complexes, BEAF-32/CP190, or BEAF-32/Chromator, indicating that these proteins may play an analogous role in flies as that of CTCF/cohesin in mammals. Moreover, extended regions previously thought to be unstructured are shown to consist of small contiguous TADs, a property also observed in mammals upon re-examination. Altogether, our work demonstrates that fundamental features associated with the higher-order folding of the genome are conserved from insects to mammals.
[Mh] Termos MeSH primário: Cromatina/ultraestrutura
Mapeamento Cromossômico/métodos
Cromossomos de Insetos/ultraestrutura
Drosophila melanogaster/genética
Genoma de Inseto
Mamíferos/genética
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Fator de Ligação a CCCTC/genética
Fator de Ligação a CCCTC/metabolismo
Proteínas de Ciclo Celular/genética
Proteínas de Ciclo Celular/metabolismo
Cromatina/química
Montagem e Desmontagem da Cromatina
Proteínas Cromossômicas não Histona/genética
Proteínas Cromossômicas não Histona/metabolismo
Mapeamento Cromossômico/instrumentação
Cromossomos de Insetos/química
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/ultraestrutura
Proteínas do Olho/genética
Proteínas do Olho/metabolismo
Expressão Gênica
Seres Humanos
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Conformação Molecular
Proteínas Associadas à Matriz Nuclear/genética
Proteínas Associadas à Matriz Nuclear/metabolismo
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (BEAF-32 protein, Drosophila); 0 (CCCTC-Binding Factor); 0 (CP190 protein, Drosophila); 0 (CTCF protein, human); 0 (Cell Cycle Proteins); 0 (Chromatin); 0 (Chromosomal Proteins, Non-Histone); 0 (DNA-Binding Proteins); 0 (Drosophila Proteins); 0 (Eye Proteins); 0 (Microtubule-Associated Proteins); 0 (Nuclear Matrix-Associated Proteins); 0 (Nuclear Proteins); 0 (chromator protein, Drosophila); 0 (cohesins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02526-9


  6 / 5191 MEDLINE  
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[PMID]:28468672
[Au] Autor:Arbona JM; Herbert S; Fabre E; Zimmer C
[Ad] Endereço:Unité Imagerie et Modélisation, Institut Pasteur, 25 rue du Docteur Roux, 75015, Paris, France.
[Ti] Título:Inferring the physical properties of yeast chromatin through Bayesian analysis of whole nucleus simulations.
[So] Source:Genome Biol;18(1):81, 2017 05 03.
[Is] ISSN:1474-760X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The structure and mechanical properties of chromatin impact DNA functions and nuclear architecture but remain poorly understood. In budding yeast, a simple polymer model with minimal sequence-specific constraints and a small number of structural parameters can explain diverse experimental data on nuclear architecture. However, how assumed chromatin properties affect model predictions was not previously systematically investigated. RESULTS: We used hundreds of dynamic chromosome simulations and Bayesian inference to determine chromatin properties consistent with an extensive dataset that includes hundreds of measurements from imaging in fixed and live cells and two Hi-C studies. We place new constraints on average chromatin fiber properties, narrowing down the chromatin compaction to ~53-65 bp/nm and persistence length to ~52-85 nm. These constraints argue against a 20-30 nm fiber as the exclusive chromatin structure in the genome. Our best model provides a much better match to experimental measurements of nuclear architecture and also recapitulates chromatin dynamics measured on multiple loci over long timescales. CONCLUSION: This work substantially improves our understanding of yeast chromatin mechanics and chromosome architecture and provides a new analytic framework to infer chromosome properties in other organisms.
[Mh] Termos MeSH primário: Núcleo Celular/genética
Montagem e Desmontagem da Cromatina
Cromatina/química
Simulação por Computador
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Teorema de Bayes
Cromatina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromatin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s13059-017-1199-x


  7 / 5191 MEDLINE  
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[PMID]:29368470
[Au] Autor:Mazina MY; Vorobyeva NE
[Ti] Título:[The role of ATP-dependent chromatin remodeling complexes in regulation of genetic processes].
[So] Source:Genetika;52(5):529-40, 2016 May.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Compaction of the genomic DNA into the chromatin structure reduces the accessibility of DNAbinding protein sites and complicates the realization of replication and transcription. In the cell, the negative effects of DNA condensation into chromatin are overcome by recruiting the complexes that change the chromatin structure and are involved in the regulation of transcription and replication. The chromatin remodeling process includes the alteration of nucleosome position and chromatin density and changes in the histone composition of the nucleosomes. ATP-dependent chromatin remodeling is performed by enzymes­chromatin remodeling complexes. The united activity of these enzymes forms the dynamic properties of chromatin during different nuclear processes such as transcription, replication, DNA repair, homological recombination, and chromatin assembly. In this review, we summarize the currently available data on the structure of chromatin remodeling complexes of different families, the pathways of their recruitment to certain chromatin sites, and their functional activity.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Montagem e Desmontagem da Cromatina/fisiologia
Nucleossomos/metabolismo
[Mh] Termos MeSH secundário: Animais
Reparo do DNA/fisiologia
Replicação do DNA/fisiologia
Recombinação Homóloga/fisiologia
Seres Humanos
Nucleossomos/genética
Transcrição Genética/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nucleosomes); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  8 / 5191 MEDLINE  
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[PMID]:29196537
[Au] Autor:Kawasumi R; Abe T; Arakawa H; Garre M; Hirota K; Branzei D
[Ad] Endereço:The FIRC (Italian Foundation for Cancer Research) Institute of Molecular Oncology (IFOM), 20139 Milan, Italy.
[Ti] Título:ESCO1/2's roles in chromosome structure and interphase chromatin organization.
[So] Source:Genes Dev;31(21):2136-2150, 2017 11 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ESCO1/2 acetyltransferases mediating SMC3 acetylation and sister chromatid cohesion (SCC) are differentially required for genome integrity and development. Here we established chicken DT40 cell lines with mutations in ESCO1/2, SMC3 acetylation, and the cohesin remover WAPL. Both ESCO1 and ESCO2 promoted SCC, while ESCO2 was additionally and specifically required for proliferation and centromere integrity. overexpression fully suppressed the slow proliferation and centromeric separation phenotypes of cells but only partly suppressed its chromosome arm SCC defects. Concomitant inactivation of ESCO1 and ESCO2 caused lethality owing to compromised mitotic chromosome segregation. Neither nor acetyl-mimicking mutations rescued lethality. Notably, conditional mutants showed very severe proliferation defects associated with catastrophic mitoses and also abnormal interphase chromatin organization patterns. The results indicate that cohesion establishment by vertebrate ESCO1/2 is linked to interphase chromatin architecture formation, a newly identified function of cohesin acetyltransferases that is both fundamentally and medically relevant.
[Mh] Termos MeSH primário: Acetiltransferases/metabolismo
Montagem e Desmontagem da Cromatina/genética
Proteínas Cromossômicas não Histona/metabolismo
Estruturas Cromossômicas/genética
Instabilidade Genômica/genética
[Mh] Termos MeSH secundário: Acetilação
Acetiltransferases/genética
Animais
Linhagem Celular
Proliferação Celular/genética
Centrômero/genética
Galinhas
Proteínas Cromossômicas não Histona/genética
Técnicas de Inativação de Genes
Inativação Gênica
Interfase/genética
Proteínas Nucleares/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (Nuclear Proteins); EC 2.3.1.- (Acetyltransferases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE
[do] DOI:10.1101/gad.306084.117


  9 / 5191 MEDLINE  
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[PMID]:29180489
[Au] Autor:Kaminuma O; Kitamura N; Nishito Y; Nemoto S; Tatsumi H; Mori A; Hiroi T
[Ad] Endereço:Allergy and Immunology Project, The Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan; osamuk@yamanashi.ac.jp.
[Ti] Título:Downregulation of NFAT3 Due to Lack of T-Box Transcription Factor TBX5 Is Crucial for Cytokine Expression in T Cells.
[So] Source:J Immunol;200(1):92-100, 2018 01 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The NFAT family transcription factors play crucial roles in immunological and other biological activities. NFAT3 is rarely expressed in T cells, and the mechanisms and significance of the specific NFAT3 downregulation in T cells have been unknown. In human CD4 T cells, overexpression of NFAT1 and NFAT3 enhanced and suppressed IL-2 expression, respectively. NFAT3 downregulation in Jurkat cells using RNA interference technology augmented IL-2 expression, whereas a knockdown of NFAT1, NFAT2, and NFAT4 suppressed it. The promoter/enhancer activity of the NFAT-binding site in the gene was upregulated and downregulated by NFAT1 and NFAT3, respectively. A study employing NFAT1/NFAT3 chimeric molecules revealed that the region in NFAT3 responsible for NFAT promoter activity inhibition was located within its N-terminal transactivation domain, Ca -regulatory domain, and DNA-binding domain. Downregulation of NFAT3 expression in T cells is mediated by lower chromatin accessibility and enhancer activity in its promoter in comparison with aortic smooth muscle cells expressing endogenous NFAT3. The binding sites of T-box transcription factor TBX5 and NK-2 transcription factor-related locus 5 Nkx2.5, which were expressed at higher levels in aortic smooth muscle cells than in T cells, were located within the -387 to +97 NFAT3 promoter region, exhibiting the maximum enhancer activity. Mutating the binding site of TBX5 but not Nkx2.5 diminished the NFAT3 promoter activity, whereas the overexpression of TBX5 enhanced it. Introduction of TBX5 into CD4 T cells enhanced the expression of NFAT3 and suppressed that of IL-2. TBX5 deficiency-mediated downregulation of NFAT3 is crucial for the high cytokine-producing activity of T cells.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Citocinas/metabolismo
Miócitos de Músculo Liso/fisiologia
Fatores de Transcrição NFATC/metabolismo
Proteínas com Domínio T-Box/metabolismo
[Mh] Termos MeSH secundário: Aorta/patologia
Células Cultivadas
Montagem e Desmontagem da Cromatina
Citocinas/genética
Regulação da Expressão Gênica
Proteína Homeobox Nkx-2.5/genética
Proteína Homeobox Nkx-2.5/metabolismo
Seres Humanos
Interleucina-2/genética
Interleucina-2/metabolismo
Fatores de Transcrição NFATC/genética
Regiões Promotoras Genéticas/genética
Ligação Proteica
Engenharia de Proteínas
Proteínas com Domínio T-Box/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Homeobox Protein Nkx-2.5); 0 (Interleukin-2); 0 (NFATC Transcription Factors); 0 (NKX2-5 protein, human); 0 (T-Box Domain Proteins); 0 (T-box transcription factor 5)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1602113


  10 / 5191 MEDLINE  
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[PMID]:28464892
[Au] Autor:Li B; Gale RP; Xu Z; Qin T; Song Z; Zhang P; Bai J; Zhang L; Zhang Y; Liu J; Huang G; Xiao Z
[Ad] Endereço:MDS and MPN Centre, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin, 300020, China.
[Ti] Título:Non-driver mutations in myeloproliferative neoplasm-associated myelofibrosis.
[So] Source:J Hematol Oncol;10(1):99, 2017 May 02.
[Is] ISSN:1756-8722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We studied non-driver mutations in 62 subjects with myeloproliferative neoplasm (MPN)-associated myelofibrosis upon diagnosis, including 45 subjects with primary myelofibrosis (PMF) and 17 with post-polycythemia vera or post-essential thrombocythemia myelofibrosis (post-PV/ET MF). Fifty-eight subjects had ≥1 non-driver mutation upon diagnosis. Mutations in mRNA splicing genes, especially in U2AF1, were significantly more frequent in PMF than in post-PV/ET MF (33 vs. 6%; P = 0.015). There were also striking differences in clonal architecture. These data indicate different genomic spectrums between PMF and post-PV/ET MF.
[Mh] Termos MeSH primário: Mutação
Policitemia Vera/genética
Mielofibrose Primária/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Apoptose/genética
Adesão Celular/genética
Ciclo Celular/genética
Montagem e Desmontagem da Cromatina/genética
Células Clonais
Metilação de DNA/genética
Reparo do DNA/genética
Feminino
Seres Humanos
Masculino
Meia-Idade
Policitemia Vera/complicações
Mielofibrose Primária/etiologia
Processamento de RNA/genética
Transdução de Sinais/genética
Fator de Processamento U2AF/genética
Trombocitemia Essencial/complicações
Trombocitemia Essencial/genética
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; LETTER
[Nm] Nome de substância:
0 (Splicing Factor U2AF); 0 (U2AF1 protein, human)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s13045-017-0472-5



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