Base de dados : MEDLINE
Pesquisa : G04.580.100 [Categoria DeCS]
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  1 / 80614 MEDLINE  
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[PMID]:29358717
[Au] Autor:Malagon-Vina H; Ciocchi S; Passecker J; Dorffner G; Klausberger T
[Ad] Endereço:Center for Brain Research, Divison of Cognitive Neurobiology, Medical University of Vienna, 1090, Vienna, Austria. hugo.malagonvina@meduniwien.ac.at.
[Ti] Título:Fluid network dynamics in the prefrontal cortex during multiple strategy switching.
[So] Source:Nat Commun;9(1):309, 2018 01 22.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Coordinated shifts of neuronal activity in the prefrontal cortex are associated with strategy adaptations in behavioural tasks, when animals switch from following one rule to another. However, network dynamics related to multiple-rule changes are scarcely known. We show how firing rates of individual neurons in the prelimbic and cingulate cortex correlate with the performance of rats trained to change their navigation multiple times according to allocentric and egocentric strategies. The concerted population activity exhibits a stable firing during the performance of one rule but shifted to another neuronal firing state when a new rule is learnt. Interestingly, when the same rule is presented a second time within the same session, neuronal firing does not revert back to the original neuronal firing state, but a new activity-state is formed. Our data indicate that neuronal firing of prefrontal cortical neurons represents changes in strategy and task-performance rather than specific strategies or rules.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Rede Nervosa/fisiologia
Neurônios/fisiologia
Córtex Pré-Frontal/fisiologia
[Mh] Termos MeSH secundário: Algoritmos
Animais
Giro do Cíngulo/fisiologia
Aprendizagem em Labirinto/fisiologia
Modelos Neurológicos
Ratos Long-Evans
Navegação Espacial/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02764-x


  2 / 80614 MEDLINE  
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[PMID]:29351552
[Au] Autor:Kropf J; Rössler W
[Ad] Endereço:Behavioral Physiology and Sociobiology (Zoology II), Biozentrum, University of Würzburg, Würzburg, Germany.
[Ti] Título:In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
[So] Source:PLoS One;13(1):e0191425, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The honeybee olfactory pathway comprises an intriguing pattern of convergence and divergence: ~60.000 olfactory sensory neurons (OSN) convey olfactory information on ~900 projection neurons (PN) in the antennal lobe (AL). To transmit this information reliably, PNs employ relatively high spiking frequencies with complex patterns. PNs project via a dual olfactory pathway to the mushroom bodies (MB). This pathway comprises the medial (m-ALT) and the lateral antennal lobe tract (l-ALT). PNs from both tracts transmit information from a wide range of similar odors, but with distinct differences in coding properties. In the MBs, PNs form synapses with many Kenyon cells (KC) that encode odors in a spatially and temporally sparse way. The transformation from complex information coding to sparse coding is a well-known phenomenon in insect olfactory coding. Intrinsic neuronal properties as well as GABAergic inhibition are thought to contribute to this change in odor representation. In the present study, we identified intrinsic neuronal properties promoting coding differences between PNs and KCs using in-situ patch-clamp recordings in the intact brain. We found very prominent K+ currents in KCs clearly differing from the PN currents. This suggests that odor coding differences between PNs and KCs may be caused by differences in their specific ion channel properties. Comparison of ionic currents of m- and l-ALT PNs did not reveal any differences at a qualitative level.
[Mh] Termos MeSH primário: Abelhas/citologia
Abelhas/fisiologia
Condutos Olfatórios/citologia
Condutos Olfatórios/fisiologia
[Mh] Termos MeSH secundário: Potenciais de Ação
Animais
Antenas de Artrópodes/citologia
Antenas de Artrópodes/fisiologia
Encéfalo/citologia
Encéfalo/fisiologia
Fenômenos Eletrofisiológicos
Canais Iônicos/fisiologia
Transporte de Íons
Corpos Pedunculados/citologia
Corpos Pedunculados/fisiologia
Neurônios Receptores Olfatórios/citologia
Neurônios Receptores Olfatórios/fisiologia
Técnicas de Patch-Clamp
Olfato/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ion Channels)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191425


  3 / 80614 MEDLINE  
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[PMID]:28465084
[Au] Autor:Jensen TP; Zheng K; Tyurikova O; Reynolds JP; Rusakov DA
[Ad] Endereço:UCL Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK. Electronic address: t.jensen@ucl.ac.uk.
[Ti] Título:Monitoring single-synapse glutamate release and presynaptic calcium concentration in organised brain tissue.
[So] Source:Cell Calcium;64:102-108, 2017 Jun.
[Is] ISSN:1532-1991
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Brain function relies in large part on Ca -dependent release of the excitatory neurotransmitter glutamate from neuronal axons. Establishing the causal relationship between presynaptic Ca dynamics and probabilistic glutamate release is therefore a fundamental quest across neurosciences. Its progress, however, has hitherto depended primarily on the exploration of either cultured nerve cells or giant central synapses accessible to direct experimental probing in situ. Here we show that combining patch-clamp with time-resolved imaging of Ca -sensitive fluorescence lifetime of Oregon Green BAPTA-1 (Tornado-FLIM) enables readout of single spike-evoked presynaptic Ca concentration dynamics, with nanomolar sensitivity, in individual neuronal axons in acute brain slices. In parallel, intensity Tornado imaging of a locally expressed extracellular optical glutamate sensor iGluSnFr provides direct monitoring of single-quantum, single-synapse glutamate releases in situ. These two methods pave the way for simultaneous registration of presynaptic Ca dynamics and transmitter release in an intact brain at the level of individual synapses.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Cálcio/metabolismo
Ácido Glutâmico/metabolismo
Terminações Pré-Sinápticas/metabolismo
Sinapses/metabolismo
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Compostos de Anilina/metabolismo
Animais
Axônios/metabolismo
Fluoresceínas/metabolismo
Hipocampo/metabolismo
Camundongos Endogâmicos C57BL
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Fluoresceins); 0 (Oregon green 488 BAPTA-1); 3KX376GY7L (Glutamic Acid); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  4 / 80614 MEDLINE  
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[PMID]:28451637
[Au] Autor:Sun Y; Grieco SF; Holmes TC; Xu X
[Ad] Endereço:Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, CA 92697-1275.
[Ti] Título:Local and Long-Range Circuit Connections to Hilar Mossy Cells in the Dentate Gyrus.
[So] Source:eNeuro;4(2), 2017 Mar-Apr.
[Is] ISSN:2373-2822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hilar mossy cells are the prominent glutamatergic cell type in the dentate hilus of the dentate gyrus (DG); they have been proposed to have critical roles in the DG network. To better understand how mossy cells contribute to DG function, we have applied new viral genetic and functional circuit mapping approaches to quantitatively map and compare local and long-range circuit connections of mossy cells and dentate granule cells in the mouse. The great majority of inputs to mossy cells consist of two parallel inputs from within the DG: an excitatory input pathway from dentate granule cells and an inhibitory input pathway from local DG inhibitory neurons. Mossy cells also receive a moderate degree of excitatory and inhibitory CA3 input from proximal CA3 subfields. Long range inputs to mossy cells are numerically sparse, and they are only identified readily from the medial septum and the septofimbrial nucleus. In comparison, dentate granule cells receive most of their inputs from the entorhinal cortex. The granule cells receive significant synaptic inputs from the hilus and the medial septum, and they also receive direct inputs from both distal and proximal CA3 subfields, which has been underdescribed in the existing literature. Our slice-based physiological mapping studies further supported the identified circuit connections of mossy cells and granule cells. Together, our data suggest that hilar mossy cells are major local circuit integrators and they exert modulation of the activity of dentate granule cells as well as the CA3 region through "back-projection" pathways.
[Mh] Termos MeSH primário: Hipocampo/citologia
Fibras Musgosas Hipocampais/anatomia & histologia
[Mh] Termos MeSH secundário: Potenciais de Ação
Animais
Neurônios Colinérgicos/citologia
Feminino
Neurônios GABAérgicos/citologia
Hipocampo/fisiologia
Masculino
Camundongos Endogâmicos C57BL
Fibras Musgosas Hipocampais/fisiologia
Inibição Neural
Vias Neurais/citologia
Vias Neurais/fisiologia
Técnicas de Rastreamento Neuroanatômico
Núcleos Septais/citologia
Sinapses
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  5 / 80614 MEDLINE  
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[PMID]:29352276
[Au] Autor:Osadchii OE
[Ad] Endereço:Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
[Ti] Título:Arrhythmogenic drugs can amplify spatial heterogeneities in the electrical restitution in perfused guinea-pig heart: An evidence from assessments of monophasic action potential durations and JT intervals.
[So] Source:PLoS One;13(1):e0191514, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Non-uniform shortening of the action potential duration (APD90) in different myocardial regions upon heart rate acceleration can set abnormal repolarization gradients and promote arrhythmia. This study examined whether spatial heterogeneities in APD90 restitution can be amplified by drugs with clinically proved proarrhythmic potential (dofetilide, quinidine, procainamide, and flecainide) and, if so, whether these effects can translate to the appropriate changes of the ECG metrics of ventricular repolarization, such as JT intervals. In isolated, perfused guinea-pig heart preparations, monophasic action potentials and volume-conducted ECG were recorded at progressively increased pacing rates. The APD90 measured at distinct ventricular sites, as well as the JTpeak and JTend values were plotted as a function of preceding diastolic interval, and the maximum slopes of the restitution curves were determined at baseline and upon drug administration. Dofetilide, quinidine, and procainamide reverse rate-dependently prolonged APD90 and steepened the restitution curve, with effects being greater at the endocardium than epicardium, and in the right ventricular (RV) vs. the left ventricular (LV) chamber. The restitution slope was increased to a greater extent for the JTend vs. the JTpeak interval. In contrast, flecainide reduced the APD90 restitution slope at LV epicardium without producing effect at LV endocardium and RV epicardium, and reduced the JTpeak restitution slope without changing the JTend restitution. Nevertheless, with all agents, these effects translated to the amplified epicardial-to-endocardial and the LV-to-RV non-uniformities in APD90 restitution, paralleled by the increased JTend vs. JTpeak difference in the restitution slope. In summary, these findings suggest that arrhythmic drug profiles are partly attributable to the accentuated regional heterogeneities in APD90 restitution, which can be indirectly determined through ECG assessments of the JTend vs. JTpeak dynamics at variable pacing rates.
[Mh] Termos MeSH primário: Antiarrítmicos/efeitos adversos
Arritmias Cardíacas/induzido quimicamente
Arritmias Cardíacas/fisiopatologia
[Mh] Termos MeSH secundário: Potenciais de Ação/efeitos dos fármacos
Potenciais de Ação/fisiologia
Animais
Fenômenos Eletrofisiológicos
Feminino
Flecainida/efeitos adversos
Cobaias
Técnicas In Vitro
Perfusão
Fenetilaminas/efeitos adversos
Procainamida/efeitos adversos
Quinidina/efeitos adversos
Sulfonamidas/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Arrhythmia Agents); 0 (Phenethylamines); 0 (Sulfonamides); ITX08688JL (Quinidine); K94FTS1806 (Flecainide); L39WTC366D (Procainamide); R4Z9X1N2ND (dofetilide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191514


  6 / 80614 MEDLINE  
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[PMID]:29337067
[Au] Autor:Yassa ME; Mansour IA; Sewelam NI; Hamza H; Gaafar T
[Ad] Endereço:Department of Clinical and Chemical Pathology, Kasr Al-Ainy School of Medicine, Cairo University, Kasr Al-Ainy St., 11562, Cairo, Egypt. Electronic address: marianne.yassa@kasralainy.edu.eg.
[Ti] Título:The impact of growth factors on human induced pluripotent stem cells differentiation into cardiomyocytes.
[So] Source:Life Sci;196:38-47, 2018 Mar 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Human induced pluripotent stem cells (hiPSCs) act as a promising therapeutic alternative for cardiovascular diseases. They yield a large number of functional cardiomyocytes (CMs) from autologous cell sources without ethical or immunological problems. However, significant limitations still remain in terms of line-to-line variability in CM yield and reproducibility. AIM: To efficiently enhance NP0040 hiPSCs differentiation into CMs. MAIN METHODS: Following a standard cardiac differentiation protocol using small molecules targeting the canonical Wnt signaling, growth factors (BMP4 and FGF2) and ascorbic acid were added further in order to increase the cardiac differentiation efficiency. All cultures were conducted in serum-free, feeder-free monolayer system followed by lactate purification. KEY FINDINGS: Using NP0040 hiPSCs, the CM yield resulting from modulation of the Wnt signaling pathway alone was inefficient compared to previous studies while the addition of BMP4, FGF2 and ascorbic acid resulted in enhanced cardiac differentiation outcome. The later resulted in a high yield (up to 92%) of cardiac troponin-T (cTnT) + CMs contracting spontaneously as organized sheets in 15 independent experiments. They were validated structurally and functionally using immunofluorescent staining for sarcomeric α-actinin, cTnT, MLC2v and Connexin 43. Reverse-transcriptase PCR revealed cardiac transcription factors and cardiac-specific genes expression. CMs were electrically connected to one another. Recorded action potential (AP) showed waves of relatively mature ventricular-like phenotype. SIGNIFICANCE: We demonstrated that hiPSC lines respond differently to a standard cardiac differentiation protocol and that a well-orchestrated interplay between Wnt, BMP4, FGF/MEK and Ascorbic acid MEK/ERK1/2 signaling pathways is beneficial in enhancing the differentiation outcome.
[Mh] Termos MeSH primário: Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos
Peptídeos e Proteínas de Sinalização Intercelular/farmacologia
Miócitos Cardíacos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Potenciais de Ação/efeitos dos fármacos
Ácido Ascórbico/farmacologia
Proteína Morfogenética Óssea 4/metabolismo
Diferenciação Celular/efeitos dos fármacos
Espaço Extracelular/efeitos dos fármacos
Expressão Gênica/efeitos dos fármacos
Glucose/deficiência
Seres Humanos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Contração Miocárdica
Miócitos Cardíacos/metabolismo
Troponina T/metabolismo
Vitaminas/farmacologia
Via de Sinalização Wnt/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 4); 0 (Intercellular Signaling Peptides and Proteins); 0 (Troponin T); 0 (Vitamins); IY9XDZ35W2 (Glucose); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


  7 / 80614 MEDLINE  
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[PMID]:29330458
[Au] Autor:Schwarz D; Kollo M; Bosch C; Feinauer C; Whiteley I; Margrie TW; Cutforth T; Schaefer AT
[Ad] Endereço:Behavioural Neurophysiology, Max Planck Institute for Medical Research, Jahnstraße 29, Heidelberg, 69120, Germany. daniel.schwarz@med.uni-heidelberg.de.
[Ti] Título:Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.
[So] Source:Nat Commun;9(1):183, 2018 01 12.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the "inputs" and "outputs," remains a major challenge. Here, we present the development of nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation of a substantial volume of neuronal tissue. Combining finite element modeling and focused ion beam milling, NEMs permit substantially higher stimulation intensities compared to conventional glass capillaries, allowing for larger volumes configurable to the geometry of the target circuit. We apply NEMs to achieve near-complete labeling of the neuronal network associated with a genetically identified olfactory glomerulus. This allows us to detect sparse higher-order features of the wiring architecture that are inaccessible to statistical labeling approaches. Thus, NEM labeling provides crucial complementary information to dense circuit reconstruction techniques. Relying solely on targeting an electrode to the region of interest and passive biophysical properties largely common across cell types, this can easily be employed anywhere in the CNS.
[Mh] Termos MeSH primário: Microeletrodos
Nanotecnologia/métodos
Neurônios/metabolismo
Bulbo Olfatório/metabolismo
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Animais
Dendritos/metabolismo
Dendritos/fisiologia
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Camundongos Transgênicos
Microscopia Confocal
Microscopia Eletrônica de Varredura
Neurônios/fisiologia
Neurônios/ultraestrutura
Bulbo Olfatório/citologia
Bulbo Olfatório/ultraestrutura
Receptores Odorantes/genética
Receptores Odorantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Receptors, Odorant); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02560-7


  8 / 80614 MEDLINE  
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[PMID]:27775722
[Au] Autor:Laurens J; Kim B; Dickman JD; Angelaki DE
[Ad] Endereço:Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA.
[Ti] Título:Gravity orientation tuning in macaque anterior thalamus.
[So] Source:Nat Neurosci;19(12):1566-1568, 2016 12.
[Is] ISSN:1546-1726
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gravity may provide a ubiquitous allocentric reference to the brain's spatial orientation circuits. Here we describe neurons in the macaque anterior thalamus tuned to pitch and roll orientation relative to gravity, independently of visual landmarks. We show that individual cells exhibit two-dimensional tuning curves, with peak firing rates at a preferred vertical orientation. These results identify a thalamic pathway for gravity cues to influence perception, action and spatial cognition.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Núcleos Anteriores do Tálamo/fisiologia
Cognição/fisiologia
Gravitação
Neurônios/fisiologia
Orientação/fisiologia
Percepção Espacial/fisiologia
[Mh] Termos MeSH secundário: Animais
Macaca
Estimulação Luminosa/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE
[do] DOI:10.1038/nn.4423


  9 / 80614 MEDLINE  
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[PMID]:29306026
[Au] Autor:So EC; Wu SN; Lo YC; Su K
[Ad] Endereço:Department of Anesthesia, An Nan Hospital, China Medical University, 70965, Tainan City, Taiwan; Department of Anesthesia, China Medical University, 40447 Taichung City, Taiwan. Electronic address: d11320@mail.tmanh.org.tw.
[Ti] Título:Differential regulation of tefluthrin and telmisartan on the gating charges of I activation and inactivation as well as on resurgent and persistent I in a pituitary cell line (GH ).
[So] Source:Toxicol Lett;285:104-112, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Voltage-gated Na currents (I ), known to contain many components (e.g., transient, resurgent and persistent I ) with unique gating properties, are involved in the generation and propagation of action potentials in excitable cells. In this study, how tefluthrin (Tef), a synthetic pyrethoid, and telmisartan (TEL), blocker of angiotensin II receptors can perturb those components of I was investigated. The presence of either Tef or TEL increased the values of the gating charges of I involved in the activation (z ) and inactivation (z ). Tef also increased the amplitude of resurgent I (I ) or persistent I (I ) in a pituitary cell line (GH ), while TEL produced minimal effects on them. Subsequent addition of either ranolazine (a blocker of late I ) or d-limonene (a monoterpene), could reverse the changes by TEL or Tef on z or z . In SCN5A-expressing HEK293T cells, addition of Tef or TEL also increased the peak amplitude and the inactivation time constant of I which was accompanied by the increased z value of I . Taken together, the results indicated that Tef- or TEL-mediated changes in the gating kinetics of I are linked to their actions on functional activity of neurons, neuroendocrine or endocrine cells.
[Mh] Termos MeSH primário: Benzimidazóis/farmacologia
Benzoatos/farmacologia
Ciclopropanos/farmacologia
Hidrocarbonetos Fluorados/farmacologia
Ativação do Canal Iônico/efeitos dos fármacos
Somatotrofos/efeitos dos fármacos
Canais de Sódio Disparados por Voltagem/metabolismo
[Mh] Termos MeSH secundário: Potenciais de Ação/efeitos dos fármacos
Animais
Linhagem Celular Tumoral
Cicloexenos/farmacologia
Células HEK293
Seres Humanos
Canal de Sódio Disparado por Voltagem NAV1.5/genética
Canal de Sódio Disparado por Voltagem NAV1.5/metabolismo
Ranolazina/farmacologia
Ratos
Somatotrofos/metabolismo
Terpenos/farmacologia
Transfecção
Canais de Sódio Disparados por Voltagem/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzimidazoles); 0 (Benzoates); 0 (Cyclohexenes); 0 (Cyclopropanes); 0 (Hydrocarbons, Fluorinated); 0 (NAV1.5 Voltage-Gated Sodium Channel); 0 (SCN5A protein, human); 0 (Terpenes); 0 (Voltage-Gated Sodium Channels); 2HE8P42H2J (2,3,5,6-tetrafluoro-4-methylbenzyl (Z)-(1RS)-cis-3-(2-chloro-3,3,3-trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylate); 9MC3I34447 (limonene); A6IEZ5M406 (Ranolazine); U5SYW473RQ (telmisartan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  10 / 80614 MEDLINE  
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[PMID]:29371650
[Au] Autor:Sheikhbahaei S; Turovsky EA; Hosford PS; Hadjihambi A; Theparambil SM; Liu B; Marina N; Teschemacher AG; Kasparov S; Smith JC; Gourine AV
[Ad] Endereço:Centre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, UK.
[Ti] Título:Astrocytes modulate brainstem respiratory rhythm-generating circuits and determine exercise capacity.
[So] Source:Nat Commun;9(1):370, 2018 01 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Astrocytes are implicated in modulation of neuronal excitability and synaptic function, but it remains unknown if these glial cells can directly control activities of motor circuits to influence complex behaviors in vivo. This study focused on the vital respiratory rhythm-generating circuits of the preBötzinger complex (preBötC) and determined how compromised function of local astrocytes affects breathing in conscious experimental animals (rats). Vesicular release mechanisms in astrocytes were disrupted by virally driven expression of either the dominant-negative SNARE protein or light chain of tetanus toxin. We show that blockade of vesicular release in preBötC astrocytes reduces the resting breathing rate and frequency of periodic sighs, decreases rhythm variability, impairs respiratory responses to hypoxia and hypercapnia, and dramatically reduces the exercise capacity. These findings indicate that astrocytes modulate the activity of CNS circuits generating the respiratory rhythm, critically contribute to adaptive respiratory responses in conditions of increased metabolic demand and determine the exercise capacity.
[Mh] Termos MeSH primário: Astrócitos/fisiologia
Tronco Encefálico/fisiologia
Periodicidade
Condicionamento Físico Animal/fisiologia
Respiração
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Adenoviridae/genética
Adenoviridae/metabolismo
Animais
Animais Recém-Nascidos
Astrócitos/citologia
Tronco Encefálico/citologia
Cálcio/metabolismo
Feminino
Regulação da Expressão Gênica
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Hipercapnia/metabolismo
Hipercapnia/fisiopatologia
Hipóxia/metabolismo
Hipóxia/fisiopatologia
Masculino
Bulbo/citologia
Bulbo/fisiologia
Cultura Primária de Células
Ratos
Ratos Sprague-Dawley
Proteínas SNARE/antagonistas & inibidores
Proteínas SNARE/genética
Proteínas SNARE/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (SNARE Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02723-6



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