Base de dados : MEDLINE
Pesquisa : G05.308.320 [Categoria DeCS]
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[PMID]:29378241
[Au] Autor:Zhang L; Wang MY; Li XP; Wang XT; Jia CL; Yang XZ; Feng RQ; Yuan ML
[Ad] Endereço:State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, People's Republic of China.
[Ti] Título:A small set of differentially expressed genes was associated with two color morphs in natural populations of the pea aphid Acyrthosiphon pisum.
[So] Source:Gene;651:23-32, 2018 Apr 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Color polymorphism is an ecologically important trait, which is related to local adaptation and ecological speciation. The pea aphid Acyrthosiphon pisum shows color polymorphism: the red and green color morphs where differences in ecological adaptation have been observed. Here, we measured genome-wide gene expression profiles of two color morphs in natural populations of A. pisum to explore the genetic basis of differentiated ecological adaptation. The results showed that only 32 genes were significantly differentially expressed between the two morphs, of which 18 had functional annotations. Among them, 13 genes were up-regulated [e.g. genes encoding protoheme IX farnesyltransferase (LOC100570971), carotene dehydrogenase (tor) and V-type proton ATPase subunit B (LOC100169462)] and 5 genes were down-regulated in the red morph (e.g. genes encoding transcription factors and heat shock proteins). To assess the functional importance of these differentially expressed genes (DEGs), we selected three highly expressed DEGs (LOC100169462, LOC100570971 and tor) with functional annotations and analyzed their expression levels in the red morph under three low temperatures (1 °C, 4 °C, and 8 °C) for 24 h. These three DEGs showed an interesting expression response to the cold acclimating conditions which resulted in an obvious phenotypic change of the red individuals to be greenish variants. This study suggests a link between gene expressions and body color polymorphisms in the pea aphid and provides important clues for further studying molecular mechanisms of ecological adaptation in aphids.
[Mh] Termos MeSH primário: Afídeos/genética
Genes de Insetos
[Mh] Termos MeSH secundário: Animais
Temperatura Baixa
Regulação Enzimológica da Expressão Gênica
Ontologia Genética
Medicago sativa
Pigmentação/genética
Polimorfismo Genético
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de RNA
Transcriptoma
beta-Caroteno 15,15'-Mono-Oxigenase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.99.36 (beta-Carotene 15,15'-Monooxygenase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE


  2 / 41663 MEDLINE  
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[PMID]:28822946
[Au] Autor:Xu ZR; Cai SW; Huang WX; Liu RX; Xiong ZT
[Ad] Endereço:School of Resource and Environmental Sciences, Wuhan University, Wuhan, Hubei, People's Republic of China.
[Ti] Título:Differential expression of vacuolar and defective cell wall invertase genes in roots and seeds of metalliferous and non-metalliferous populations of Rumex dentatus under copper stress.
[So] Source:Ecotoxicol Environ Saf;147:17-25, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Acid invertase activities in roots and young seeds of a metalliferous population (MP) of Rumex dentatus were previously observed to be significantly higher than those of a non-metalliferous population (NMP) under Cu stress. To date, no acid invertase gene has been cloned from R. dentatus. Here, we isolated four full-length cDNAs from the two populations of R. dentatus, presumably encoding cell wall (RdnCIN1 and RdmCIN1 from the NMP and MP, respectively) and vacuolar invertases (RdnVIN1 and RdmVIN1 from the NMP and MP, respectively). Unexpectedly, RdnCIN1 and RdmCIN1 most likely encode special defective invertases with highly attenuated sucrose-hydrolyzing capacity. The transcript levels of RdmCIN1 were significantly higher than those of RdnCIN1 in roots and young seeds under Cu stress, whereas under control conditions, the former was initially lower than the latter. Unexpected high correlations were observed between the transcript levels of RdnCIN1 and RdmCIN1 and the activity of cell wall invertase, even though RdnCIN1 and RdmCIN1 do not encode catalytically active invertases. Similarly, the transcript levels of RdmVIN1 in roots and young seeds were increased under Cu stress, whereas those of RdnVIN1 were decreased. The high correlations between the transcript levels of RdnVIN1 and RdmVIN1 and the activity of vacuolar invertase indicate that RdnVIN1 and RdmVIN1 might control distinct vacuolar invertase activities in the two populations. Moreover, a possible indirect role for acid invertases in Cu tolerance, mediated by generating a range of sugars used as nutrients and signaling molecules, is discussed.
[Mh] Termos MeSH primário: Parede Celular/efeitos dos fármacos
Cobre/toxicidade
Rumex/efeitos dos fármacos
Poluentes do Solo/toxicidade
Vacúolos/efeitos dos fármacos
beta-Frutofuranosidase/genética
[Mh] Termos MeSH secundário: Parede Celular/enzimologia
Parede Celular/genética
Cobre/metabolismo
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Genes de Plantas
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/enzimologia
Raízes de Plantas/metabolismo
Rumex/genética
Rumex/metabolismo
Sementes/efeitos dos fármacos
Sementes/enzimologia
Sementes/genética
Poluentes do Solo/metabolismo
Vacúolos/enzimologia
Vacúolos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 789U1901C5 (Copper); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170821
[St] Status:MEDLINE


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[PMID]:27779297
[Au] Autor:Han KH; Kim MA; Park NH
[Ad] Endereço:Department of Obstetrics and Gynecology, Seoul National University Hospital Healthcare System Gangnam Center, Seoul, Republic of Korea.
[Ti] Título:Expression of aurora kinases: Predictor of tumor dissemination in uterine carcinosarcoma.
[So] Source:Histol Histopathol;32(7):717-724, 2017 Jul.
[Is] ISSN:1699-5848
[Cp] País de publicação:Spain
[La] Idioma:eng
[Ab] Resumo:Uterine carcinosarcoma is a rare, aggressive, and biphasic tumor. It comprises carcinomatous and sarcomatous components, and mitosis-associated factors are thought to discriminate these two lesions. Aurora kinases are mitotic enzymes that are highly expressed in uterine malignancies. To identify the clinical significance of aurora kinase expression, we performed immunohistochemistry on tissue microarrays using cores selected from areas with typical carcinomatous and sarcomatous characteristics. A total of 24 samples were included, from patients at Seoul National University Hospital diagnosed with uterine carcinosarcoma, and who undergone a staging operation between 1997 and 2012. Patients' clinical and pathological data were analyzed, and expression patterns of aurora kinases were investigated. Aurora kinases A and B were dominantly expressed in the cytoplasm, and phospho-aurora kinases A and B were expressed in the nuclei. Phospho-aurora kinase A and aurora kinase B showed significantly higher expression in the carcinomatous component (P=0.012 and 0.008). High expression of phospho-aurora kinase A was associated with lymphatic metastasis such as positive pelvic lymph node and omental involvement (P=0.012 and 0.037). Overexpression of aurora kinase B was related to vascular invasion (P=0.011). High expression of both phospho-aurora kinase A and aurora kinase B was a prognostic factor for progression-free survival in uterine carcinosarcoma (P=0.049). In conclusion, expression of aurora kinases is associated with bidirectional tumor dissemination into the lymphatic and hematogenous pathways. In addition, high expression of phospho-aurora kinase A and aurora kinase B is a predictor of progression-free survival. Therefore, inhibitors of aurora kinases might be a prospective therapeutic options for uterine carcinosarcoma.
[Mh] Termos MeSH primário: Aurora Quinases/biossíntese
Carcinossarcoma/enzimologia
Carcinossarcoma/patologia
Neoplasias Uterinas/enzimologia
Neoplasias Uterinas/patologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Aurora Quinase A/metabolismo
Aurora Quinase B/metabolismo
Aurora Quinases/genética
Biomarcadores Tumorais
Intervalo Livre de Doença
Feminino
Regulação Enzimológica da Expressão Gênica/genética
Regulação Neoplásica da Expressão Gênica/genética
Seres Humanos
Imuno-Histoquímica
Estimativa de Kaplan-Meier
Menopausa
Meia-Idade
Metástase Neoplásica/patologia
Estadiamento de Neoplasias
Valor Preditivo dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); EC 2.7.11.1 (AURKA protein, human); EC 2.7.11.1 (AURKB protein, human); EC 2.7.11.1 (Aurora Kinase A); EC 2.7.11.1 (Aurora Kinase B); EC 2.7.11.1 (Aurora Kinases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.14670/HH-11-834


  4 / 41663 MEDLINE  
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[PMID]:29348663
[Au] Autor:Miao Y; Ajami NE; Huang TS; Lin FM; Lou CH; Wang YT; Li S; Kang J; Munkacsi H; Maurya MR; Gupta S; Chien S; Subramaniam S; Chen Z
[Ad] Endereço:Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope, 1500 Duarte Rd., Duarte, CA, 91010, USA.
[Ti] Título:Enhancer-associated long non-coding RNA LEENE regulates endothelial nitric oxide synthase and endothelial function.
[So] Source:Nat Commun;9(1):292, 2018 01 18.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The optimal expression of endothelial nitric oxide synthase (eNOS), the hallmark of endothelial homeostasis, is vital to vascular function. Dynamically regulated by various stimuli, eNOS expression is modulated at transcriptional, post-transcriptional, and post-translational levels. However, epigenetic modulations of eNOS, particularly through long non-coding RNAs (lncRNAs) and chromatin remodeling, remain to be explored. Here we identify an enhancer-associated lncRNA that enhances eNOS expression (LEENE). Combining RNA-sequencing and chromatin conformation capture methods, we demonstrate that LEENE is co-regulated with eNOS and that its enhancer resides in proximity to eNOS promoter in endothelial cells (ECs). Gain- and Loss-of-function of LEENE differentially regulate eNOS expression and EC function. Mechanistically, LEENE facilitates the recruitment of RNA Pol II to the eNOS promoter to enhance eNOS nascent RNA transcription. Our findings unravel a new layer in eNOS regulation and provide novel insights into cardiovascular regulation involving endothelial function.
[Mh] Termos MeSH primário: Células Endoteliais/metabolismo
Elementos Facilitadores Genéticos/genética
Regulação Enzimológica da Expressão Gênica
Óxido Nítrico Sintase Tipo III/genética
RNA Longo não Codificante/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Perfilação da Expressão Gênica
Seres Humanos
Masculino
Camundongos Endogâmicos C57BL
Óxido Nítrico Sintase Tipo III/metabolismo
Regiões Promotoras Genéticas/genética
RNA Polimerase II/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Long Noncoding); 0 (long non-coding RNA LEENE, human); EC 1.14.13.39 (Nitric Oxide Synthase Type III); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02113-y


  5 / 41663 MEDLINE  
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[PMID]:28461448
[Au] Autor:Borchert AJ; Downs DM
[Ad] Endereço:Department of Microbiology, University of Georgia, Athens, Georgia, USA.
[Ti] Título:The Response to 2-Aminoacrylate Differs in Escherichia coli and Salmonella enterica, despite Shared Metabolic Components.
[So] Source:J Bacteriol;199(14), 2017 07 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The metabolic network of an organism includes the sum total of the biochemical reactions present. In microbes, this network has an impeccable ability to sense and respond to perturbations caused by internal or external stimuli. The metabolic potential (i.e., network structure) of an organism is often drawn from the genome sequence, based on the presence of enzymes deemed to indicate specific pathways. and are members of the family of Gram-negative bacteria that share the majority of their metabolic components and regulatory machinery as the "core genome." In , the ability of the enamine intermediate 2-aminoacrylate (2AA) to inactivate a number of pyridoxal 5'-phosphate (PLP)-dependent enzymes has been established In this study, 2AA metabolism and the consequences of its accumulation were investigated in The data showed that despite the conservation of all relevant enzymes, and differed in both the generation and detrimental consequences of 2AA. In total, these findings suggest that the structure of the metabolic network surrounding the generation and response to endogenous 2AA stress differs between and This work compared the metabolic networks surrounding the endogenous stressor 2-aminoacrylate in two closely related members of the The data showed that despite the conservation of all relevant enzymes in this metabolic node, the two closely related organisms diverged in their metabolic network structures. This work highlights how a set of conserved components can generate distinct network architectures and how this can impact the physiology of an organism. This work defines a model to expand our understanding of the 2-aminoacrylate stress response and the differences in metabolic structures and cellular milieus between and .
[Mh] Termos MeSH primário: Acrilatos/farmacologia
Proteínas de Bactérias/metabolismo
Escherichia coli/efeitos dos fármacos
Salmonella enterica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adenina/farmacologia
Ácido Aspártico/farmacologia
Proteínas de Bactérias/genética
Escherichia coli/metabolismo
Deleção de Genes
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Regulação Enzimológica da Expressão Gênica/fisiologia
L-Serina Desidratase/genética
L-Serina Desidratase/metabolismo
Salmonella enterica/metabolismo
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Acrylates); 0 (Bacterial Proteins); 30KYC7MIAI (Aspartic Acid); EC 4.3.1.17 (L-Serine Dehydratase); JAC85A2161 (Adenine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  6 / 41663 MEDLINE  
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[PMID]:28461447
[Au] Autor:Hoffmann MC; Pfänder Y; Tintel M; Masepohl B
[Ad] Endereço:Biologie der Mikroorganismen, Fakultät für Biologie und Biotechnologie, Ruhr-Universität Bochum, Bochum, Germany.
[Ti] Título:Bacterial PerO Permeases Transport Sulfate and Related Oxyanions.
[So] Source:J Bacteriol;199(14), 2017 07 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:synthesizes the high-affinity ABC transporters CysTWA and ModABC to specifically import the chemically related oxyanions sulfate and molybdate, respectively. In addition, has the low-affinity permease PerO acting as a general oxyanion transporter, whose elimination increases tolerance to molybdate and tungstate. Although PerO-like permeases are widespread in bacteria, their function has not been examined in any other species to date. Here, we present evidence that PerO permeases from the alphaproteobacteria , , , and and the gammaproteobacterium functionally substitute for PerO in sulfate uptake and sulfate-dependent growth, as shown by assimilation of radioactively labeled sulfate and heterologous complementation. Disruption of genes in , , and increased tolerance to tungstate and, in the case of , to molybdate, suggesting that heterometal oxyanions are common substrates of PerO permeases. This study supports the view that bacterial PerO permeases typically transport sulfate and related oxyanions and, hence, form a functionally conserved permease family. Despite the widespread distribution of PerO-like permeases in bacteria, our knowledge about PerO function until now was limited to one species, In this study, we showed that PerO proteins from diverse bacteria are functionally similar to the prototype, suggesting that PerO permeases form a conserved family whose members transport sulfate and related oxyanions.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Proteínas de Membrana Transportadoras/metabolismo
Rhodobacter capsulatus/enzimologia
Sulfatos/metabolismo
[Mh] Termos MeSH secundário: Ânions/metabolismo
Proteínas de Bactérias/genética
Transporte Biológico/fisiologia
Regulação Bacteriana da Expressão Gênica/fisiologia
Regulação Enzimológica da Expressão Gênica/fisiologia
Proteínas de Membrana Transportadoras/genética
Mutação
Rhodobacter capsulatus/genética
Rhodobacter capsulatus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anions); 0 (Bacterial Proteins); 0 (Membrane Transport Proteins); 0 (Sulfates)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  7 / 41663 MEDLINE  
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[PMID]:28457994
[Au] Autor:Kady N; Yan Y; Salazar T; Wang Q; Chakravarthy H; Huang C; Beli E; Navitskaya S; Grant M; Busik J
[Ad] Endereço:Department of Physiology, Michigan State University, East Lansing, MI, USA.
[Ti] Título:Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34 circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes.
[So] Source:J Clin Lipidol;11(3):694-703, 2017 May - Jun.
[Is] ISSN:1933-2874
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Diabetic retinopathy is a microvascular disease that results from retinal vascular degeneration and defective repair due to diabetes-induced endothelial progenitor dysfunction. OBJECTIVE: Understanding key molecular factors involved in vascular degeneration and repair is paramount for developing effective diabetic retinopathy treatment strategies. We propose that diabetes-induced activation of acid sphingomyelinase (ASM) plays essential role in retinal endothelial and CD34 circulating angiogenic cell (CAC) dysfunction in diabetes. METHODS: Human retinal endothelial cells (HRECs) isolated from control and diabetic donor tissue and human CD34 CACs from control and diabetic patients were used in this study. ASM messenger RNA and protein expression were assessed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. To evaluate the effect of diabetes-induced ASM on HRECs and CD34 CACs function, tube formation, CAC incorporation into endothelial tubes, and diurnal release of CD34 CACs in diabetic individuals were determined. RESULTS: ASM expression level was significantly increased in HRECs isolated from diabetic compared with control donor tissue, as well as CD34 CACs and plasma of diabetic patients. A significant decrease in tube area was observed in HRECs from diabetic donors compared with control HRECs. The tube formation deficiency was associated with increased expression of ASM in diabetic HRECs. Moreover, diabetic CD34 CACs with high ASM showed defective incorporation into endothelial tubes. Diurnal release of CD34 CACs was disrupted with the rhythmicity lost in diabetic patients. CONCLUSION: Collectively, these findings support that diabetes-induced ASM upregulation has a marked detrimental effect on both retinal endothelial cells and CACs.
[Mh] Termos MeSH primário: Retinopatia Diabética/enzimologia
Células Endoteliais/metabolismo
Neovascularização Patológica/patologia
Retina/patologia
Vasos Retinianos/fisiopatologia
Esfingomielina Fosfodiesterase/metabolismo
[Mh] Termos MeSH secundário: Idoso
Antígenos CD34/metabolismo
Ritmo Circadiano
Retinopatia Diabética/sangue
Retinopatia Diabética/patologia
Retinopatia Diabética/fisiopatologia
Células Endoteliais/patologia
Feminino
Regulação Enzimológica da Expressão Gênica
Seres Humanos
Masculino
Meia-Idade
Vasos Retinianos/metabolismo
Esfingomielina Fosfodiesterase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD34); EC 3.1.4.- (acid sphingomyelinase-1); EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  8 / 41663 MEDLINE  
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[PMID]:29416026
[Au] Autor:Wang YQ; Wang HL; Xu J; Tan J; Fu LN; Wang JL; Zou TH; Sun DF; Gao QY; Chen YX; Fang JY
[Ad] Endereço:Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Middle Shandong Road, Shanghai, 200001, China.
[Ti] Título:Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
[So] Source:Nat Commun;9(1):545, 2018 02 07.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Reversible post-translational modifications represent a mechanism to control tumor metabolism. Here we show that mitochondrial Sirtuin5 (SIRT5), which mediates lysine desuccinylation, deglutarylation, and demalonylation, plays a role in colorectal cancer (CRC) glutamine metabolic rewiring. Metabolic profiling identifies that deletion of SIRT5 causes a marked decrease in C-glutamine incorporation into tricarboxylic-acid (TCA) cycle intermediates and glutamine-derived non-essential amino acids. This reduces the building blocks required for rapid growth. Mechanistically, the direct interaction between SIRT5 and glutamate dehydrogenase 1 (GLUD1) causes deglutarylation and functional activation of GLUD1, a critical regulator of cellular glutaminolysis. Consistently, GLUD1 knockdown diminishes SIRT5-induced proliferation, both in vivo and in vitro. Clinically, overexpression of SIRT5 is significantly correlated with poor prognosis in CRC. Thus, SIRT5 supports the anaplerotic entry of glutamine into the TCA cycle in malignant phenotypes of CRC via activating GLUD1.
[Mh] Termos MeSH primário: Carcinogênese/metabolismo
Neoplasias Colorretais/metabolismo
Regulação Neoplásica da Expressão Gênica/fisiologia
Glutamato Desidrogenase/metabolismo
Glutamina/metabolismo
Sirtuínas/metabolismo
[Mh] Termos MeSH secundário: Proliferação Celular
Ciclo do Ácido Cítrico/fisiologia
Regulação Enzimológica da Expressão Gênica/fisiologia
Glutamato Desidrogenase/genética
Células HCT116
Seres Humanos
Interferência de RNA
Sirtuínas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0RH81L854J (Glutamine); EC 1.4.1.2 (Glutamate Dehydrogenase); EC 1.4.1.3 (GLUD1 protein, human); EC 3.5.1.- (SIRT5 protein, human); EC 3.5.1.- (Sirtuins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180209
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-018-02951-4


  9 / 41663 MEDLINE  
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[PMID]:29351555
[Au] Autor:Xue Y; Chen B; Win AN; Fu C; Lian J; Liu X; Wang R; Zhang X; Chai Y
[Ad] Endereço:College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
[Ti] Título:Omega-3 fatty acid desaturase gene family from two ω-3 sources, Salvia hispanica and Perilla frutescens: Cloning, characterization and expression.
[So] Source:PLoS One;13(1):e0191432, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Omega-3 fatty acid desaturase (ω-3 FAD, D15D) is a key enzyme for α-linolenic acid (ALA) biosynthesis. Both chia (Salvia hispanica) and perilla (Perilla frutescens) contain high levels of ALA in seeds. In this study, the ω-3 FAD gene family was systematically and comparatively cloned from chia and perilla. Perilla FAD3, FAD7, FAD8 and chia FAD7 are encoded by single-copy (but heterozygous) genes, while chia FAD3 is encoded by 2 distinct genes. Only 1 chia FAD8 sequence was isolated. In these genes, there are 1 to 6 transcription start sites, 1 to 8 poly(A) tailing sites, and 7 introns. The 5'UTRs of PfFAD8a/b contain 1 to 2 purine-stretches and 2 pyrimidine-stretches. An alternative splice variant of ShFAD7a/b comprises a 5'UTR intron. Their encoded proteins harbor an FA_desaturase conserved domain together with 4 trans-membrane helices and 3 histidine boxes. Phylogenetic analysis validated their identity of dicot microsomal or plastidial ω-3 FAD proteins, and revealed some important evolutionary features of plant ω-3 FAD genes such as convergent evolution across different phylums, single-copy status in algae, and duplication events in certain taxa. The qRT-PCR assay showed that the ω-3 FAD genes of two species were expressed at different levels in various organs, and they also responded to multiple stress treatments. The functionality of the ShFAD3 and PfFAD3 enzymes was confirmed by yeast expression. The systemic molecular and functional features of the ω-3 FAD gene family from chia and perilla revealed in this study will facilitate their use in future studies on genetic improvement of ALA traits in oilseed crops.
[Mh] Termos MeSH primário: Ácidos Graxos Dessaturases/genética
Genes de Plantas
Perilla frutescens/enzimologia
Perilla frutescens/genética
Proteínas de Plantas/genética
Salvia/enzimologia
Salvia/genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Processamento Alternativo
Sequência de Aminoácidos
Clonagem Molecular
Sequência Conservada
Evolução Molecular
Ácidos Graxos Dessaturases/química
Ácidos Graxos Dessaturases/metabolismo
Regulação Enzimológica da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Família Multigênica
Especificidade de Órgãos
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA de Plantas/genética
RNA de Plantas/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
Estresse Fisiológico
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Plant Proteins); 0 (RNA, Messenger); 0 (RNA, Plant); 0 (Recombinant Proteins); EC 1.14.19.- (Fatty Acid Desaturases); EC 1.14.99.- (omega-3 fatty acid desaturase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191432


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[PMID]:29339738
[Au] Autor:Luo N; Nixon MJ; Gonzalez-Ericsson PI; Sanchez V; Opalenik SR; Li H; Zahnow CA; Nickels ML; Liu F; Tantawy MN; Sanders ME; Manning HC; Balko JM
[Ad] Endereço:Department of Anatomy and Histology, School of Medicine, Nankai University, Tianjin, 300071, China.
[Ti] Título:DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer.
[So] Source:Nat Commun;9(1):248, 2018 01 16.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Potentiating anti-tumor immunity by inducing tumor inflammation and T cell-mediated responses are a promising area of cancer therapy. Immunomodulatory agents that promote these effects function via a wide variety of mechanisms, including upregulation of antigen presentation pathways. Here, we show that major histocompatibility class-I (MHC-I) genes are methylated in human breast cancers, suppressing their expression. Treatment of breast cancer cell lines with a next-generation hypomethylating agent, guadecitabine, upregulates MHC-I expression in response to interferon-γ. In murine tumor models of breast cancer, guadecitabine upregulates MHC-I in tumor cells promoting recruitment of CD8+ T cells to the microenvironment. Finally, we show that MHC-I genes are upregulated in breast cancer patients treated with hypomethylating agents. Thus, the immunomodulatory effects of hypomethylating agents likely involve upregulation of class-I antigen presentation to potentiate CD8+ T cell responses. These strategies may be useful to potentiate anti-tumor immunity and responses to checkpoint inhibition in immune-refractory breast cancers.
[Mh] Termos MeSH primário: Azacitidina/análogos & derivados
Neoplasias da Mama/metabolismo
DNA (Citosina-5-)-Metiltransferase 1/antagonistas & inibidores
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Genes MHC Classe I/fisiologia
Linfócitos T Citotóxicos/fisiologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacologia
Azacitidina/farmacologia
Linhagem Celular Tumoral
DNA (Citosina-5-)-Metiltransferase 1/genética
DNA (Citosina-5-)-Metiltransferase 1/metabolismo
Feminino
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Genes MHC Classe I/genética
Seres Humanos
Neoplasias Mamárias Experimentais
Camundongos
Regiões Promotoras Genéticas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 2KT4YN1DP7 (guadecitabine); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1); EC 2.1.1.37 (DNMT1 protein, human); M801H13NRU (Azacitidine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02630-w



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