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[PMID]:29220449
[Au] Autor:Choi JA; Wyrick JJ
[Ad] Endereço:School of Molecular Biosciences.
[Ti] Título:RegulatorDB: a resource for the analysis of yeast transcriptional regulation.
[So] Source:Database (Oxford);2017, 2017 Jan 01.
[Is] ISSN:1758-0463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Database URL: http://wyrickbioinfo2.smb.wsu.edu/RegulatorDB.
[Mh] Termos MeSH primário: DNA Fúngico
Proteínas de Ligação a DNA
Bases de Dados Genéticas
Proteínas Fúngicas
Regulação Fúngica da Expressão Gênica/genética
Saccharomyces cerevisiae
[Mh] Termos MeSH secundário: Sítios de Ligação
DNA Fúngico/genética
DNA Fúngico/metabolismo
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA-Binding Proteins); 0 (Fungal Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1093/database/bax058


  2 / 18052 MEDLINE  
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[PMID]:29176784
[Au] Autor:Fu C; Heitman J
[Ad] Endereço:Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, United States of America.
[Ti] Título:PRM1 and KAR5 function in cell-cell fusion and karyogamy to drive distinct bisexual and unisexual cycles in the Cryptococcus pathogenic species complex.
[So] Source:PLoS Genet;13(11):e1007113, 2017 Nov.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sexual reproduction is critical for successful evolution of eukaryotic organisms in adaptation to changing environments. In the opportunistic human fungal pathogens, the Cryptococcus pathogenic species complex, C. neoformans primarily undergoes bisexual reproduction, while C. deneoformans undergoes both unisexual and bisexual reproduction. During both unisexual and bisexual cycles, a common set of genetic circuits regulates a yeast-to-hyphal morphological transition, that produces either monokaryotic or dikaryotic hyphae. As such, both the unisexual and bisexual cycles can generate genotypic and phenotypic diversity de novo. Despite the similarities between these two cycles, genetic and morphological differences exist, such as the absence of an opposite mating-type partner and monokaryotic instead of dikaryotic hyphae during C. deneoformans unisexual cycle. To better understand the similarities and differences between these modes of sexual reproduction, we focused on two cellular processes involved in sexual reproduction: cell-cell fusion and karyogamy. We identified orthologs of the plasma membrane fusion protein Prm1 and the nuclear membrane fusion protein Kar5 in both Cryptococcus species, and demonstrated their conserved roles in cell fusion and karyogamy during C. deneoformans α-α unisexual reproduction and C. deneoformans and C. neoformans a-α bisexual reproduction. Notably, karyogamy occurs inside the basidum during bisexual reproduction in C. neoformans, but often occurs earlier following cell fusion during bisexual reproduction in C. deneoformans. Characterization of these two genes also showed that cell fusion is dispensable for solo unisexual reproduction in C. deneoformans. The blastospores produced along hyphae during C. deneoformans unisexual reproduction are diploid, suggesting that diploidization occurs early during hyphal development, possibly through either an endoreplication pathway or cell fusion-independent karyogamy events. Taken together, our findings suggest distinct mating mechanisms for unisexual and bisexual reproduction in Cryptococcus, exemplifying distinct evolutionary trajectories within this pathogenic species complex.
[Mh] Termos MeSH primário: Cryptococcus neoformans/genética
Proteínas Fúngicas/genética
Genes Fúngicos Tipo Acasalamento/genética
Reprodução Assexuada/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Núcleo Celular/genética
Núcleo Celular/metabolismo
Cryptococcus neoformans/citologia
Cryptococcus neoformans/crescimento & desenvolvimento
Diploide
Regulação da Expressão Gênica no Desenvolvimento
Regulação Fúngica da Expressão Gênica
Hifas/genética
Hifas/crescimento & desenvolvimento
Fusão de Membrana/genética
Microscopia Confocal
Modelos Genéticos
Mutação
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007113


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[PMID]:29295978
[Au] Autor:Jung B; Park J; Kim N; Li T; Kim S; Bartley LE; Kim J; Kim I; Kang Y; Yun K; Choi Y; Lee HH; Ji S; Lee KS; Kim BY; Shon JC; Kim WC; Liu KH; Yoon D; Kim S; Seo YS; Lee J
[Ad] Endereço:Department of Applied Biology, Dong-A University, Busan, 49315, Korea.
[Ti] Título:Cooperative interactions between seed-borne bacterial and air-borne fungal pathogens on rice.
[So] Source:Nat Commun;9(1):31, 2018 01 02.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacterial-fungal interactions are widely found in distinct environments and contribute to ecosystem processes. Previous studies of these interactions have mostly been performed in soil, and only limited studies of aerial plant tissues have been conducted. Here we show that a seed-borne plant pathogenic bacterium, Burkholderia glumae (Bg), and an air-borne plant pathogenic fungus, Fusarium graminearum (Fg), interact to promote bacterial survival, bacterial and fungal dispersal, and disease progression on rice plants, despite the production of antifungal toxoflavin by Bg. We perform assays of toxoflavin sensitivity, RNA-seq analyses, lipid staining and measures of triacylglyceride content to show that triacylglycerides containing linolenic acid mediate resistance to reactive oxygen species that are generated in response to toxoflavin in Fg. As a result, Bg is able to physically attach to Fg to achieve rapid and expansive dispersal to enhance disease severity.
[Mh] Termos MeSH primário: Microbiologia do Ar
Burkholderia/fisiologia
Fusarium/fisiologia
Oryza/microbiologia
Sementes/microbiologia
[Mh] Termos MeSH secundário: Burkholderia/metabolismo
Farmacorresistência Fúngica/efeitos dos fármacos
Fusarium/classificação
Fusarium/genética
Perfilação da Expressão Gênica
Regulação Fúngica da Expressão Gênica
Interações Hospedeiro-Patógeno
Interações Microbianas
Mutação
Filogenia
Doenças das Plantas/microbiologia
Pirimidinonas/metabolismo
Pirimidinonas/farmacologia
Triazinas/metabolismo
Triazinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pyrimidinones); 0 (Triazines); 5N5YI4IP1P (toxoflavin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02430-2


  4 / 18052 MEDLINE  
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[PMID]:29339748
[Au] Autor:Lee S; Oh S; Jeong K; Jo H; Choi Y; Seo HD; Kim M; Choe J; Kwon CS; Lee D
[Ad] Endereço:Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.
[Ti] Título:Dot1 regulates nucleosome dynamics by its inherent histone chaperone activity in yeast.
[So] Source:Nat Commun;9(1):240, 2018 01 16.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dot1 (disruptor of telomeric silencing-1, DOT1L in humans) is the only known enzyme responsible for histone H3 lysine 79 methylation (H3K79me) and is evolutionarily conserved in most eukaryotes. Yeast Dot1p lacks a SET domain and does not methylate free histones and thus may have different actions with respect to other histone methyltransferases. Here we show that Dot1p displays histone chaperone activity and regulates nucleosome dynamics via histone exchange in yeast. We show that a methylation-independent function of Dot1p is required for the cryptic transcription within transcribed regions seen following disruption of the Set2-Rpd3S pathway. Dot1p can assemble core histones to nucleosomes and facilitate ATP-dependent chromatin-remodeling activity through its nucleosome-binding domain, in vitro. Global analysis indicates that Dot1p appears to be particularly important for histone exchange and chromatin accessibility on the transcribed regions of long-length genes. Our findings collectively suggest that Dot1p-mediated histone chaperone activity controls nucleosome dynamics in transcribed regions.
[Mh] Termos MeSH primário: Chaperonas de Histonas/metabolismo
Histona-Lisina N-Metiltransferase/metabolismo
Proteínas Nucleares/metabolismo
Nucleossomos/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Cromatina/genética
Cromatina/metabolismo
Regulação Fúngica da Expressão Gênica
Chaperonas de Histonas/genética
Histona-Lisina N-Metiltransferase/genética
Histonas/metabolismo
Lisina/metabolismo
Mutação
Proteínas Nucleares/genética
Nucleossomos/genética
Ligação Proteica
Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/genética
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromatin); 0 (Histone Chaperones); 0 (Histones); 0 (Nuclear Proteins); 0 (Nucleosomes); 0 (Saccharomyces cerevisiae Proteins); EC 2.1.1.43 (Dot1 protein, S cerevisiae); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02759-8


  5 / 18052 MEDLINE  
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[PMID]:27773677
[Au] Autor:Jeronimo C; Langelier MF; Bataille AR; Pascal JM; Pugh BF; Robert F
[Ad] Endereço:Institut de recherches cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada.
[Ti] Título:Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo.
[So] Source:Mol Cell;64(3):455-466, 2016 Nov 03.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mediator is a highly conserved transcriptional coactivator organized into four modules, namely Tail, Middle, Head, and Kinase (CKM). Previous work suggests regulatory roles for Tail and CKM, but an integrated model for these activities is lacking. Here, we analyzed the genome-wide distribution of Mediator subunits in wild-type and mutant yeast cells in which RNA polymerase II promoter escape is blocked, allowing detection of transient Mediator forms. We found that although all modules are recruited to upstream activated regions (UAS), assembly of Mediator within the pre-initiation complex is accompanied by the release of CKM. Interestingly, our data show that CKM regulates Mediator-UAS interaction rather than Mediator-promoter association. In addition, although Tail is required for Mediator recruitment to UAS, Tailless Mediator nevertheless interacts with core promoters. Collectively, our data suggest that the essential function of Mediator is mediated by Head and Middle at core promoters, while Tail and CKM play regulatory roles.
[Mh] Termos MeSH primário: Regulação Fúngica da Expressão Gênica
Complexo Mediador/genética
RNA Polimerase II/genética
Proteínas de Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/genética
Fator de Transcrição TFIIB/genética
[Mh] Termos MeSH secundário: Sítios de Ligação
Complexo Mediador/metabolismo
Modelos Moleculares
Regiões Promotoras Genéticas
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
RNA Polimerase II/metabolismo
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Fator de Transcrição TFIIB/metabolismo
Iniciação da Transcrição Genética
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mediator Complex); 0 (Protein Subunits); 0 (SUA7 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factor TFIIB); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


  6 / 18052 MEDLINE  
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[PMID]:29371596
[Au] Autor:Duch A; Canal B; Barroso SI; García-Rubio M; Seisenbacher G; Aguilera A; de Nadal E; Posas F
[Ad] Endereço:Cell Signaling Research Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), E-08003, Barcelona, Spain.
[Ti] Título:Multiple signaling kinases target Mrc1 to prevent genomic instability triggered by transcription-replication conflicts.
[So] Source:Nat Commun;9(1):379, 2018 01 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Conflicts between replication and transcription machineries represent a major source of genomic instability and cells have evolved strategies to prevent such conflicts. However, little is known regarding how cells cope with sudden increases of transcription while replicating. Here, we report the existence of a general mechanism for the protection of genomic integrity upon transcriptional outbursts in S phase that is mediated by Mrc1. The N-terminal phosphorylation of Mrc1 blocked replication and prevented transcription-associated recombination (TAR) and genomic instability during stress-induced gene expression in S phase. An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses. Mrc1 function was not restricted to environmental cues but was also required when unscheduled transcription was triggered by low fitness states such as genomic instability or slow growth. Our data indicate that Mrc1 integrates multiple signals, thereby defining a general safeguard mechanism to protect genomic integrity upon transcriptional outbursts.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/genética
Replicação do DNA
Regulação Fúngica da Expressão Gênica
Instabilidade Genômica
Proteínas Serina-Treonina Quinases/genética
Proteínas de Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Glucose/deficiência
Temperatura Alta
Peróxido de Hidrogênio/farmacologia
Pressão Osmótica
Estresse Oxidativo/genética
Fosforilação
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Fase S
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Transdução de Sinais
Cloreto de Sódio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (MRC1 protein, S cerevisiae); 0 (Recombinant Proteins); 0 (Saccharomyces cerevisiae Proteins); 451W47IQ8X (Sodium Chloride); BBX060AN9V (Hydrogen Peroxide); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02756-x


  7 / 18052 MEDLINE  
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[PMID]:29326041
[Au] Autor:Xiao C; Yu Q; Zhang B; Li J; Zhang D; Li M
[Ad] Endereço:Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.
[Ti] Título:Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans.
[So] Source:Biochem Biophys Res Commun;496(2):253-259, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In eukaryotes, the nuclear export of mRNAs is essential for gene expression. However, little is known about the role of mRNA nuclear export in the important fungal pathogen, Candida albicans. In this study, we identified C. albicans Sus1, a nucleus-localized protein that is required for mRNA export. Interestingly, the sus1Δ/Δ displayed hyper-sensitivity to extracellular oxidative stress, enhanced ROS accumulation and severe oxidative stress-related cell death. More strikingly, although the mutant exhibited normal activation of the expression of oxidative stress response (OSR) genes, it had attenuated activity of ROS scavenging system, which may be attributed to the defect in OSR mRNA export in this mutant. In addition, the virulence of the sus1Δ/Δ was seriously attenuated. Taken together, our findings provide evidence that the mRNA export factor Sus1 plays an important role in oxidative stress tolerance and pathogenesis.
[Mh] Termos MeSH primário: Candida albicans/genética
Candida albicans/patogenicidade
Regulação Fúngica da Expressão Gênica
Proteínas Nucleares/genética
Estresse Oxidativo/genética
Proteínas de Ligação a RNA/genética
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular
Candida albicans/metabolismo
Núcleo Celular/metabolismo
Deleção de Genes
Viabilidade Microbiana
Proteínas Nucleares/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteínas de Ligação a RNA/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Estresse Fisiológico
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nuclear Proteins); 0 (RNA, Messenger); 0 (RNA-Binding Proteins); 0 (Reactive Oxygen Species)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  8 / 18052 MEDLINE  
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[PMID]:29293626
[Au] Autor:Xiang Q; Li J; Qin P; He M; Yu X; Zhao K; Zhang X; Ma M; Chen Q; Chen X; Zeng X; Gu Y
[Ad] Endereço:College of Resource, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China.
[Ti] Título:Identification and evaluation of reference genes for qRT-PCR studies in Lentinula edodes.
[So] Source:PLoS One;13(1):e0190226, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lentinula edodes (shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. It contains various medically important molecules, such as polysaccharides, terpenoids, sterols, and lipids, were contained in this mushroom. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to analyze the mechanisms underlying the biosynthetic pathways of these substances. qRT-PCR is used for accurate analyses of transcript levels owing to its rapidity, sensitivity, and reliability. However, its accuracy and reliability for the quantification of transcripts rely on the expression stability of the reference genes used for data normalization. To ensure the reliability of gene expression analyses using qRT-PCR in L. edodes molecular biology research, it is necessary to systematically evaluate reference genes. In the current study, ten potential reference genes were selected from L. edodes genomic data and their expression levels were measured by qRT-PCR using various samples. The expression stability of each candidate gene was analyzed by three commonly used software packages: geNorm, NormFinder, and BestKeeper. Base on the results, Rpl4 was the most stable reference gene across all experimental conditions, and Atu was the most stable gene among strains. 18S was found to be the best reference gene for different development stages, and Rpl4 was the most stably expressed gene under various nutrient conditions. The present work will contribute to qRT-PCR studies in L. edodes.
[Mh] Termos MeSH primário: Genes Fúngicos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Cogumelos Shiitake/genética
[Mh] Termos MeSH secundário: Regulação Fúngica da Expressão Gênica
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190226


  9 / 18052 MEDLINE  
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[PMID]:28460114
[Au] Autor:Reynolds HT; Slot JC; Divon HH; Lysøe E; Proctor RH; Brown DW
[Ad] Endereço:Department of Plant Pathology, The Ohio State University, Columbus, OH.
[Ti] Título:Differential Retention of Gene Functions in a Secondary Metabolite Cluster.
[So] Source:Mol Biol Evol;34(8):2002-2015, 2017 Aug 01.
[Is] ISSN:1537-1719
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In fungi, distribution of secondary metabolite (SM) gene clusters is often associated with host- or environment-specific benefits provided by SMs. In the plant pathogen Alternaria brassicicola (Dothideomycetes), the DEP cluster confers an ability to synthesize the SM depudecin, a histone deacetylase inhibitor that contributes weakly to virulence. The DEP cluster includes genes encoding enzymes, a transporter, and a transcription regulator. We investigated the distribution and evolution of the DEP cluster in 585 fungal genomes and found a wide but sporadic distribution among Dothideomycetes, Sordariomycetes, and Eurotiomycetes. We confirmed DEP gene expression and depudecin production in one fungus, Fusarium langsethiae. Phylogenetic analyses suggested 6-10 horizontal gene transfers (HGTs) of the cluster, including a transfer that led to the presence of closely related cluster homologs in Alternaria and Fusarium. The analyses also indicated that HGTs were frequently followed by loss/pseudogenization of one or more DEP genes. Independent cluster inactivation was inferred in at least four fungal classes. Analyses of transitions among functional, pseudogenized, and absent states of DEP genes among Fusarium species suggest enzyme-encoding genes are lost at higher rates than the transporter (DEP3) and regulatory (DEP6) genes. The phenotype of an experimentally-induced DEP3 mutant of Fusarium did not support the hypothesis that selective retention of DEP3 and DEP6 protects fungi from exogenous depudecin. Together, the results suggest that HGT and gene loss have contributed significantly to DEP cluster distribution, and that some DEP genes provide a greater fitness benefit possibly due to a differential tendency to form network connections.
[Mh] Termos MeSH primário: Alcadienos/metabolismo
Compostos de Epóxi/metabolismo
Álcoois Graxos/metabolismo
Genoma Fúngico/genética
Família Multigênica/genética
[Mh] Termos MeSH secundário: Ascomicetos/genética
Bases de Dados de Ácidos Nucleicos
Evolução Molecular
Proteínas Fúngicas/genética
Fusarium/genética
Perfilação da Expressão Gênica/métodos
Regulação Fúngica da Expressão Gênica/genética
Transferência Genética Horizontal/genética
Filogenia
Metabolismo Secundário/genética
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkadienes); 0 (Epoxy Compounds); 0 (Fatty Alcohols); 0 (Fungal Proteins); 139508-73-9 (depudecin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/molbev/msx145


  10 / 18052 MEDLINE  
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[PMID]:29247800
[Au] Autor:Sarkar S; Chakravorty S; Mukherjee A; Bhattacharya D; Bhattacharya S; Gachhui R
[Ad] Endereço:Department of Life Science & Biotechnology, Jadavpur University, India.
[Ti] Título:De novo RNA-Seq based transcriptome analysis of Papiliotrema laurentii strain RY1 under nitrogen starvation.
[So] Source:Gene;645:146-156, 2018 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nitrogen is a key nutrient for all cell forms. Most organisms respond to nitrogen scarcity by slowing down their growth rate. On the contrary, our previous studies have shown that Papiliotrema laurentii strain RY1 has a robust growth under nitrogen starvation. To understand the global regulation that leads to such an extraordinary response, we undertook a de novo approach for transcriptome analysis of the yeast. Close to 33 million sequence reads of high quality for nitrogen limited and enriched condition were generated using Illumina NextSeq500. Trinity analysis and clustered transcripts annotation of the reads produced 17,611 unigenes, out of which 14,157 could be annotated. Gene Ontology term analysis generated 44.92% cellular component terms, 39.81% molecular function terms and 15.24% biological process terms. The most over represented pathways in general were translation, carbohydrate metabolism, amino acid metabolism, general metabolism, folding, sorting, degradation followed by transport and catabolism, nucleotide metabolism, replication and repair, transcription and lipid metabolism. A total of 4256 Single Sequence Repeats were identified. Differential gene expression analysis detected 996 P-significant transcripts to reveal transmembrane transport, lipid homeostasis, fatty acid catabolism and translation as the enriched terms which could be essential for Papiliotrema laurentii strain RY1 to adapt during nitrogen deprivation. Transcriptome data was validated by quantitative real-time PCR analysis of twelve transcripts. To the best of our knowledge, this is the first report of Papiliotrema laurentii strain RY1 transcriptome which would play a pivotal role in understanding the biochemistry of the yeast under acute nitrogen stress and this study would be encouraging to initiate extensive investigations into this Papiliotrema system.
[Mh] Termos MeSH primário: Agaricales/crescimento & desenvolvimento
Proteínas Fúngicas/genética
Perfilação da Expressão Gênica/métodos
Nitrogênio/metabolismo
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Agaricales/enzimologia
Agaricales/genética
Regulação Fúngica da Expressão Gênica
Sequenciamento de Nucleotídeos em Larga Escala
Redes e Vias Metabólicas
Repetições de Microssatélites
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); N762921K75 (Nitrogen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE



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