Base de dados : MEDLINE
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[PMID]:28966124
[Au] Autor:Gan HM; Tan MH; Lee YP; Schultz MB; Horwitz P; Burnham Q; Austin CM
[Ad] Endereço:Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3220, Australia; Genomics Facility, Tropical and Medicine Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia; School
[Ti] Título:More evolution underground: Accelerated mitochondrial substitution rate in Australian burrowing freshwater crayfishes (Decapoda: Parastacidae).
[So] Source:Mol Phylogenet Evol;118:88-98, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To further understand the evolutionary history and mitogenomic features of Australia's highly distinctive freshwater crayfish fauna, we utilized a recently described rapid mitogenome sequencing pipeline to generate 24 new crayfish mitogenomes including a diversity of burrowing crayfish species and the first for Astacopsis gouldi, the world's largest freshwater invertebrate. Whole mitogenome-based phylogeny estimates using both Bayesian and Maximum Likelihood methods substantially strengthen existing hypotheses for systematic relationships among Australian freshwater crayfish with evidence of pervasive diversifying selection and accelerated mitochondrial substitution rate among the members of the clade representing strongly burrowing crayfish that may reflect selection pressures for increased energy requirement for adaptation to terrestrial environment and a burrowing lifestyle. Further, gene rearrangements are prevalent in the burrowing crayfish mitogenomes involving both tRNA and protein coding genes. In addition, duplicated control regions were observed in two closely related Engaeus species, together with evidence for concerted evolution. This study significantly adds to the understanding of Australian freshwater crayfish evolutionary relationships and suggests a link between mitogenome evolution and adaptation to terrestrial environments and a burrowing lifestyle in freshwater crayfish.
[Mh] Termos MeSH primário: Astacoidea/classificação
DNA Mitocondrial/genética
Evolução Molecular
[Mh] Termos MeSH secundário: Animais
Astacoidea/genética
Austrália
Teorema de Bayes
Códon
DNA/química
DNA/isolamento & purificação
DNA/metabolismo
DNA Mitocondrial/química
DNA Mitocondrial/classificação
DNA Mitocondrial/metabolismo
Água Doce
Ordem dos Genes
Funções Verossimilhança
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (DNA, Mitochondrial); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


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[PMID]:28988127
[Au] Autor:Solovieva EV; Myakinina VP; Kislichkina AA; Krasilnikova VM; Verevkin VV; Mochalov VV; Lev AI; Fursova NK; Volozhantsev NV
[Ad] Endereço:State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Moscow Region, Russia.
[Ti] Título:Comparative genome analysis of novel Podoviruses lytic for hypermucoviscous Klebsiella pneumoniae of K1, K2, and K57 capsular types.
[So] Source:Virus Res;243:10-18, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hypermucoviscous (HV) strains of capsular types K1, K2 and K57 are the most virulent representatives of the Klebsiella pneumoniae species. Eight novel bacteriophages lytic for HV K. pneumoniae were isolated and characterized. Three bacteriophages, KpV41, KpV475, and KpV71 were found to have a lytic activity against mainly K. pneumoniae of capsular type K1. Two phages, KpV74, and KpV763 were lytic for K2 capsular type K. pneumoniae, and the phage KpV767 was specific to K57-type K. pneumoniae only. Two more phages, KpV766, and KpV48 had no capsular specificity. The phage genomes consist of a linear double-stranded DNA of 40,395-44,623bp including direct terminal repeats of 180-246 bp. The G + C contents are 52.3-54.2 % that is slightly lower than that of genomes of K. pneumoniae strains being used for phage propagation. According to the genome structures, sequence similarity and phylogenetic data, the phages are classified within the genus Kp32virus and Kp34virus of subfamily Autographivirinae, family Podoviridae. In the phage genomes, genes encoding proteins with putative motifs of polysaccharide depolymerase were identified. Depolymerase genes of phages KpV71 and KpV74 lytic for hypermucoviscous K. pneumoniae of K1 and K2 capsular type, respectively, were cloned and expressed in Escherichia coli, and the recombinant gene products were purified. The specificity and polysaccharide-degrading activity of the recombinant depolymerases were demonstrated.
[Mh] Termos MeSH primário: Bacteriófagos/isolamento & purificação
Genoma Viral
Klebsiella pneumoniae/virologia
Podoviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Cápsulas Bacterianas/genética
Cápsulas Bacterianas/metabolismo
Bacteriófagos/classificação
Bacteriófagos/genética
Ordem dos Genes
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/metabolismo
Filogenia
Podoviridae/classificação
Podoviridae/genética
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


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[PMID]:29307851
[Au] Autor:Bai J; Xu S; Nie Z; Wang Y; Zhu C; Wang Y; Min W; Cai Y; Zou J; Zhou X
[Ad] Endereço:Research lab of Freshwater Crustacean Decapoda &Paragonimus, School of Basic Medical Sciences, Nanchang University, 461 Bayi Avenue, Nanchang City, Jiangxi Province 330006, People's Republic of China.
[Ti] Título:The complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) with phylogenetic implications for freshwater crabs.
[So] Source:Gene;646:217-226, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present study, we determined the complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) for the first time. The genome is 15,380bp in length and typically consists of 37 genes. When the gene order was compared to the ancestral crustacean type, two tRNA genes (tRNA and tRNA ) were rearranged in H. lichuanense, and the translocation of tRNA appeared only in Potamoidea crabs, such as Geothelphusa dehaani and Sinopotamon xiushuiense, supporting the monophyly of the Potamoidea superfamily. Thirteen protein-coding genes and 2 rRNA genes were divided into five complexes to perform the phylogenetic analysis, and the results showed that the trees constructed by complex I (ND1-ND6 and ND4L), complex IV (COX1-COX3) and rRNA genes better accord with the morphological classification system, suggesting that molecular markers of higher-level phylogeny can be developed in these three complexes in the future. The estimated divergence time for freshwater crabs is approximately 133.58Ma, and G. dehaani from Japan diverged from the freshwater crabs of mainland China approximately 60.66Ma. A selective pressure analysis based on current data revealed obviously increasing dN/dS ratios (except for ATP6 and ND4L) of freshwater crabs, and the accumulation of nonsynonymous mutations suggests that terrestrial habitats provide a relatively relaxed selective pressure environment for this group.
[Mh] Termos MeSH primário: Braquiúros/genética
Genoma Mitocondrial
Mitocôndrias/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Animais
Evolução Molecular
Ordem dos Genes
Tamanho do Genoma
Masculino
Filogenia
RNA de Transferência/genética
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


  4 / 5366 MEDLINE  
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[PMID]:28940019
[Au] Autor:Fehér E; Kaszab E; Forró B; Bali K; Marton S; Lengyel G; Bányai K
[Ad] Endereço:Institute for Veterinary Medical Research, Centre of Agricultural Research, Hungarian Academy of Sciences, P.O. Box 18, 1581, Budapest, Hungary.
[Ti] Título:Genome sequence of a mallard duck origin cyclovirus, DuACyV-1.
[So] Source:Arch Virol;162(12):3925-3929, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The genome sequence of a novel avian cyclovirus is described in this study. The genome size and orientation of predicted genes was similar to those described in other vertebrate and insect origin cycloviruses. The greatest genome sequence identity was shared with a dragonfly cyclovirus (nt, 60.6%). Phylogenetic analysis showed marginal relatedness with another avian cyclovirus, the chicken associated cyclovirus 1. In contrast, along a short fragment of the replication-associated protein coding gene (rep) (spanning nt 1240-1710) the duck origin cyclovirus was very similar to human origin and honey bee origin rep sequences (human - TN4, 98%; honey bee - hb10, 100%). Related cyclovirus strains existing amongst various animal species living in diverse ecosystems and separated by large geographic distances show the need for additional studies to better understand the ecology and epidemiology of cycloviruses.
[Mh] Termos MeSH primário: Circoviridae/classificação
Circoviridae/genética
Patos/virologia
Genoma Viral
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Animais
Circoviridae/isolamento & purificação
Ordem dos Genes
Genes Virais
Filogenia
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3566-z


  5 / 5366 MEDLINE  
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[PMID]:28934323
[Au] Autor:Wang YC; Wang CW; Lin WC; Tsai YJ; Chang CP; Lee YJ; Lin MJ; Li C
[Ad] Endereço:Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan.
[Ti] Título:Identification, chromosomal arrangements and expression analyses of the evolutionarily conserved prmt1 gene in chicken in comparison with its vertebrate paralogue prmt8.
[So] Source:PLoS One;12(9):e0185042, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nine protein arginine methyltransferases (PRMTs) are conserved in mammals and fish. Among these, PRMT1 is the major type I PRMT for asymmetric dimethylarginine (ADMA) formation and is the most conserved and widely distributed one. Two chicken prmt1 splicing variants were assembled and confirmed by RT-PCR experiments. However, only two scaffolds containing single separate prmt1 exon with high GC contents are present in the current chicken genome assembly. Besides, prmt1 exons are scattered in separate small scaffolds in most avian species. Complete prmt1 gene has only been predicted from two falcon species with few neighboring genes. Crocodilians are considered close to the common ancestor shared by crocodilians and birds. The gene arrangements around prmt1 in American alligator are different from that in birds but are largely conserved in human. Orthologues of genes in a large segment of human chromosomal 19 around PRMT1 are missing or not assigned to the current chicken chromosomes. In comparison, prmt8, the prmt1 paralogue, is on chicken chromosome 1 with the gene arrangements downstream of prmt8 highly conserved in birds, crocodilians, and human. However, the ones upstream vary greatly in birds. Biochemically, we found that though prmt1 transcripts were detected, limited or none PRMT1 protein was present in chicken tissues. Moreover, a much higher level of PRMT8 protein was detected in chicken brain than in mouse brain. While PRMT8 is brain specific in other vertebrate species studied, low level of PRMT8 was present in chicken but not mouse liver and muscle. We also showed that the ADMA level in chicken was similar to that in mouse. This study provides the critical information of chicken PRMT1 and PRMT8 for future analyses of the function of protein arginine methyltransferases in birds.
[Mh] Termos MeSH primário: Evolução Biológica
Encéfalo/enzimologia
Aberrações Cromossômicas
Ordem dos Genes
Proteína-Arginina N-Metiltransferases/metabolismo
Vertebrados/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Galinhas
Seres Humanos
Camundongos
Proteína-Arginina N-Metiltransferases/classificação
Proteína-Arginina N-Metiltransferases/genética
Alinhamento de Sequência
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.1.1.319 (Protein-Arginine N-Methyltransferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185042


  6 / 5366 MEDLINE  
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[PMID]:28910296
[Au] Autor:Kirk IK; Weinhold N; Brunak S; Belling K
[Ad] Endereço:Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
[Ti] Título:The impact of the protein interactome on the syntenic structure of mammalian genomes.
[So] Source:PLoS One;12(9):e0179112, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Conserved synteny denotes evolutionary preserved gene order across species. It is not well understood to which degree functional relationships between genes are preserved in syntenic blocks. Here we investigate whether protein-coding genes conserved in mammalian syntenic blocks encode gene products that serve the common functional purpose of interacting at protein level, i.e. connectivity. High connectivity among protein-protein interactions (PPIs) was only moderately associated with conserved synteny on a genome-wide scale. However, we observed a smaller subset of 3.6% of all syntenic blocks with high-confidence PPIs that had significantly higher connectivity than expected by random. Additionally, syntenic blocks with high-confidence PPIs contained significantly more chromatin loops than the remaining blocks, indicating functional preservation among these syntenic blocks. Conserved synteny is typically defined by sequence similarity. In this study, we also examined whether a functional relationship, here PPI connectivity, can identify syntenic blocks independently of orthology. While orthology-based syntenic blocks with high-confident PPIs and the connectivity-based syntenic blocks largely overlapped, the connectivity-based approach identified additional syntenic blocks that were not found by conventional sequence-based methods alone. Additionally, the connectivity-based approach enabled identification of potential orthologous genes between species. Our analyses demonstrate that subsets of syntenic blocks are associated with highly connected proteins, and that PPI connectivity can be used to detect conserved synteny even if sequence conservation drifts beyond what orthology algorithms normally can identify.
[Mh] Termos MeSH primário: Cromatina/genética
Mapeamento Cromossômico/métodos
Mamíferos/genética
Mapas de Interação de Proteínas
[Mh] Termos MeSH secundário: Algoritmos
Animais
Sequência Conservada
Cães
Evolução Molecular
Ordem dos Genes
Ligação Genética
Seres Humanos
Camundongos
Pan troglodytes
Análise de Sequência de DNA/métodos
Suínos
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179112


  7 / 5366 MEDLINE  
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[PMID]:28891001
[Au] Autor:Xiang J; Fu M; Hong N; Zhai L; Xiao F; Wang G
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
[Ti] Título:Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus.
[So] Source:Arch Virol;162(12):3907-3911, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Alternaria fungi are important pathogens infecting a wide variety of organisms. Here, we report a novel double-stranded RNA (dsRNA) mycovirus named Alternaria botybirnavirus 1 (ABRV1) isolated from a phytopathogenic Alternaria sp. strain (SCFS-3) infecting a pear tree in China. ABRV1 has two dsRNA components (dsRNAs 1 and 2) with the sizes of 6,188 and 5,903 bp, containing two putative open reading frames encoding two polyproteins (202 and 192 kDa, respectively). The polyprotein encoded by ABRV1 dsRNA1 shares 41% amino acid (aa) sequence identity with the one encoded by dsRNA2 (instead of dsRNA1) of Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1). Conversely, the polyprotein encoded by ABRV1 dsRNA2 shares 46% aa sequence identity with the one (i.e., cap-pol fusion protein) encoded by SsBRV1 dsRNA1. ABRV1 has isometric spherical virus particles (~40 nm in diameter), putatively composed of the 60-, 70- and 80-kDa structural proteins. The genomic organization and phylogenetic analyses revealed that ABRV1 belongs to a newly proposed family "Botybirnaviridae", and to our knowledge, this is the first report of a botybirnavirus infecting an Alternaria sp. strain.
[Mh] Termos MeSH primário: Alternaria/virologia
Micovírus/classificação
Micovírus/isolamento & purificação
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: China
Micovírus/genética
Ordem dos Genes
Genoma Viral
Peso Molecular
Fases de Leitura Aberta
Filogenia
Pyrus/microbiologia
Vírus de RNA/genética
RNA de Cadeia Dupla/genética
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Proteínas Virais/química
Proteínas Virais/genética
Vírion/química
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3543-6


  8 / 5366 MEDLINE  
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[PMID]:28886166
[Au] Autor:Festa S; Coppotelli BM; Madueño L; Loviso CL; Macchi M; Neme Tauil RM; Valacco MP; Morelli IS
[Ad] Endereço:Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), La Plata, Argentina.
[Ti] Título:Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches.
[So] Source:PLoS One;12(9):e0184505, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31-43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).
[Mh] Termos MeSH primário: Bactérias/classificação
Bactérias/metabolismo
Biodegradação Ambiental
Consórcios Microbianos
Fenantrenos/metabolismo
[Mh] Termos MeSH secundário: Bactérias/genética
DNA Bacteriano/genética
Ordem dos Genes
Genes Bacterianos
Metagenoma
Metagenômica/métodos
Filogenia
Proteômica/métodos
Análise de Sequência de DNA
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Phenanthrenes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184505


  9 / 5366 MEDLINE  
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[PMID]:28886124
[Au] Autor:Geng P; Tian S; Yuan Z; Hu X
[Ad] Endereço:Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
[Ti] Título:Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus.
[So] Source:PLoS One;12(9):e0184572, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cereulide-producing Bacillus cereus isolates can cause serious emetic (vomiting) syndrome and even acute lethality. As mobile genetic elements, the exploration of prophages derived from emetic B. cereus isolates will help in our understanding of the genetic diversity and evolution of these pathogens. In this study, five temperate phages derived from cereulide-producing B. cereus strains were induced, with four of them undergoing genomic sequencing. Sequencing revealed that they all belong to the Siphoviridae family, but presented in different forms in their hosts. PfNC7401 and PfIS075 have typical icosahedral heads, probably existing alone as phagemids in the host with self-replicating capability in the lysogenic state. PfEFR-4, PfEFR-5, and PfATCC7953 have elongated heads, with the genomes of the former two identified as linear dsDNA, which could be integrated into the host genome during the lysogenic state. Genomic comparison of the four phages with others also derived from emetic B. cereus isolates showed similar genome structures and core genes, thus displaying host spectrum specificity. In addition, phylogenic analysis based on the complete genome and conserved tail fiber proteins of 36 Bacillus species-derived phages confirmed that the phages derived from emetic B. cereus strains were highly similar. Furthermore, one endolysin LysPfEFR-4 was cloned and showed lytic activity against all tested emetic B. cereus strains and cross-lytic activity against some other pathogenic bacteria, implying a potential to control bacterial contamination in the food supply.
[Mh] Termos MeSH primário: Fagos Bacilares/genética
Bacillus cereus/virologia
Genoma Viral
Genômica
[Mh] Termos MeSH secundário: Fagos Bacilares/classificação
Fagos Bacilares/metabolismo
Fagos Bacilares/ultraestrutura
Bacteriólise
Análise por Conglomerados
Biologia Computacional/métodos
Ordem dos Genes
Genômica/métodos
Sequenciamento de Nucleotídeos em Larga Escala
Especificidade de Hospedeiro
Filogenia
Proteômica/métodos
Ensaio de Placa Viral
Vômito/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184572


  10 / 5366 MEDLINE  
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[PMID]:28886105
[Au] Autor:Fernández-Pérez J; Nantón A; Ruiz-Ruano FJ; Camacho JPM; Méndez J
[Ad] Endereço:Grupo Xenomar, Departamento de Bioloxía, Facultade de Ciencias and CICA (Centro de Investigacións Científicas Avanzadas), Universidade da Coruña, Campus de A Zapateira, A Coruña, Spain.
[Ti] Título:First complete female mitochondrial genome in four bivalve species genus Donax and their phylogenetic relationships within the Veneroida order.
[So] Source:PLoS One;12(9):e0184464, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Four species of the genus Donax (D. semistriatus, D. trunculus, D. variegatus and D. vittatus) are common on Iberian Peninsula coasts. Nevertheless, despite their economic importance and overexploitation, scarce genetic resources are available. In this work, we newly determined the complete mitochondrial genomes of these four representatives of the family Donacidae, with the aim of contributing to unveil phylogenetic relationships within the Veneroida order, and of developing genetic markers being useful in wedge clam identification and authentication, and aquaculture stock management. PRINCIPAL FINDINGS: The complete female mitochondrial genomes of the four species vary in size from 17,044 to 17,365 bp, and encode 13 protein-coding genes (including the atp8 gene), 2 rRNAs and 22 tRNAs, all located on the same strand. A long non-coding region was identified in each of the four Donax species between cob and cox2 genes, presumably corresponding to the Control Region. The Bayesian and Maximum Likelihood phylogenetic analysis of the Veneroida order indicate that all four species of Donax form a single clade as a sister group of other bivalves within the Tellinoidea superfamily. However, although Tellinoidea is actually monophyletic, none of its families are monophyletic. CONCLUSIONS: Sequencing of complete mitochondrial genomes provides highly valuable information to establish the phylogenetic relationships within the Veneroida order. Furthermore, we provide here significant genetic resources for further research and conservation of this commercially important fishing resource.
[Mh] Termos MeSH primário: Bivalves/classificação
Bivalves/genética
Genoma Mitocondrial
Filogenia
[Mh] Termos MeSH secundário: Animais
Biologia Computacional/métodos
Feminino
Ordem dos Genes
Genômica/métodos
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Fases de Leitura Aberta
RNA Ribossômico/genética
RNA de Transferência/genética
Regiões não Traduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (Untranslated Regions); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184464



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