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[PMID]:29385200
[Au] Autor:Rout ED; Burnett RC; Labadie JD; Yoshimoto JA; Avery AC
[Ad] Endereço:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.
[Ti] Título:Preferential use of unmutated immunoglobulin heavy variable region genes in Boxer dogs with chronic lymphocytic leukemia.
[So] Source:PLoS One;13(1):e0191205, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human chronic lymphocytic leukemia (CLL) is a clinically heterogeneous disease, and immunoglobulin heavy variable region (IGHV) gene mutational status is an important prognostic marker. IGHV mutational status has not been previously examined in canine CLL. We sequenced the IGHV-D-J rearrangements from 55 canine patients with CLL, including 36 non-Boxer and 19 Boxer dogs. The majority of non-Boxers (75%) had mutated IGHV genes, whereas the majority of Boxers (79%) had unmutated IGHV genes. IGHV3-41 and IGHV3-67 gene usage was significantly higher in Boxers with CLL compared to non-Boxers. Additionally, 11 Boxers with large B-cell lymphoma and the normal IGHV repertoire of six control dogs (three Boxers and three non-Boxers) were sequenced. IGHV3-41 was preferentially used in Boxers with other forms of lymphoma and without lymphoproliferative disease. However, preferential use of unmutated IGHV genes was unique to Boxers with CLL, suggesting Boxers may be a valuable model to investigate unmutated CLL.
[Mh] Termos MeSH primário: Doenças do Cão/genética
Doenças do Cão/imunologia
Genes de Cadeia Pesada de Imunoglobulina
Região Variável de Imunoglobulina/genética
Leucemia Linfocítica Crônica de Células B/veterinária
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Estudos de Casos e Controles
Análise Mutacional de DNA
DNA de Neoplasias/genética
Cães
Feminino
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Leucemia Linfocítica Crônica de Células B/genética
Leucemia Linfocítica Crônica de Células B/imunologia
Masculino
Mutação
Homologia de Sequência do Ácido Nucleico
Especificidade da Espécie
Éxons VDJ
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Neoplasm); 0 (Immunoglobulin Variable Region)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191205


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[PMID]:28472420
[Au] Autor:McDonald TJ; Kuo L; Kuo FC
[Ad] Endereço:From the Department of Pathology, Center for Advanced Molecular Diagnostics, Brigham and Women's Hospital, Boston, MA.
[Ti] Título:Determination of VH Family Usage in B-Cell Malignancies via the BIOMED-2 IGH PCR Clonality Assay.
[So] Source:Am J Clin Pathol;147(6):549-556, 2017 Jun 01.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: To determine whether V H family usage in B-cell lymphoproliferative disorders can be deduced from polymerase chain reaction (PCR) product-length information obtained through the BIOMED-2 (Invivoscribe, San Diego, CA) clonality assay. Methods: We develop an algorithm that uses the sizing information of the BIOMED-2 immunoglobulin heavy chain (IGH) clonality assay to deduce V H family usage. PCR with family-specific primers on 51 clinical samples containing 54 rearranged alleles were used to validate the algorithm. Results: The clonal PCR products in different framework reactions contain the same NDN segment (because they are from the same allele). Subtracting the size of the framework III product from the size of the framework I and II products yields the relative position of the framework primer binding sites for the V H segment used. The V H family can be assigned with these relative positions because they are V H family specific in the BIOMED-2 assay. The V H family assigned by the algorithm was concordant with family-specific PCR results for 49 (96%) of the 51 specimens. Conclusions: We have developed an algorithm that can correctly assign V H family usage when all three BIOMED-2 framework reactions produced clonal products. Given the wide adoption of BIOMED-2 assay, the algorithm can facilitate collection of IGH V H usage data without additional cost to the laboratories.
[Mh] Termos MeSH primário: Rearranjo Gênico de Cadeia Pesada de Linfócito B
Cadeias Pesadas de Imunoglobulinas/genética
Linfoma de Células B/genética
Transtornos Linfoproliferativos/genética
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Algoritmos
Alelos
Linfócitos B/imunologia
Células Clonais
Primers do DNA/genética
Seres Humanos
Linfoma de Células B/diagnóstico
Linfoma de Células B/imunologia
Transtornos Linfoproliferativos/diagnóstico
Transtornos Linfoproliferativos/imunologia
Técnicas de Diagnóstico Molecular
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA Primers); 0 (Immunoglobulin Heavy Chains)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqx007


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[PMID]:28438896
[Au] Autor:Longo NS; Rogosch T; Zemlin M; Zouali M; Lipsky PE
[Ad] Endereço:Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
[Ti] Título:Mechanisms That Shape Human Antibody Repertoire Development in Mice Transgenic for Human Ig H and L Chain Loci.
[So] Source:J Immunol;198(10):3963-3977, 2017 May 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To determine the impact of the milieu on the development of the human B cell repertoire, we carried out a comprehensive analysis of productive and nonproductive Ig gene rearrangements from transgenic mice engineered to express single copies of the unrearranged human H chain and L chain Ig gene loci. By examining the nonproductive repertoire as an indication of the immediate product of the rearrangement machinery without an impact of selection, we discovered that the distribution of human rearrangements arising in the mouse was generally comparable to that seen in humans. However, differences between the distribution of nonproductive and productive rearrangements that reflect the impact of selection suggested species-specific selection played a role in shaping the respective repertoires. Although expression of some VH genes was similar in mouse and human (IGHV3-23, IGHV3-30, and IGHV4-59), other genes behaved differently (IGHV3-33, IGHV3-48, IGHV4-31, IGHV4-34, and IGHV1-18). Gene selection differences were also noted in L chains. Notably, nonproductive human VH rearrangements in the transgenic mice expressed shorter CDRH3 with less N addition. Even the CDRH3s in the productive rearrangements were shorter in length than those of the normal human productive repertoire. Amino acids in the CDRH3s in both species showed positive selection of tyrosines and glycines, and negative selection of leucines. The data indicate that the environment in which B cells develop can affect the expressed Ig repertoire by exerting influences on the distribution of expressed VH and VL genes and by influencing the amino acid composition of the Ag binding site.
[Mh] Termos MeSH primário: Linfócitos B/imunologia
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Rearranjo Gênico do Linfócito B
Genes de Imunoglobulinas
Cadeias Pesadas de Imunoglobulinas/genética
Região Variável de Imunoglobulina/genética
[Mh] Termos MeSH secundário: Aminoácidos/análise
Animais
Animais Geneticamente Modificados
Genes de Cadeia Pesada de Imunoglobulina
Seres Humanos
Cadeias Pesadas de Imunoglobulinas/química
Cadeias Pesadas de Imunoglobulinas/imunologia
Imunoglobulina M
Região Variável de Imunoglobulina/química
Região Variável de Imunoglobulina/imunologia
Camundongos
Família Multigênica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Immunoglobulin Heavy Chains); 0 (Immunoglobulin M); 0 (Immunoglobulin Variable Region)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700133


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[PMID]:28419517
[Au] Autor:Cavalli M; De Novi LA; Della Starza I; Cappelli LV; Nunes V; Pulsoni A; Del Giudice I; Guarini A; Foà R
[Ad] Endereço:Haematology, Department of Cellular Biotechnologies and Haematology, Policlinico Umberto I, Sapienza University, Rome, Italy.
[Ti] Título:Comparative analysis between RQ-PCR and digital droplet PCR of BCL2/IGH gene rearrangement in the peripheral blood and bone marrow of early stage follicular lymphoma.
[So] Source:Br J Haematol;177(4):588-596, 2017 May.
[Is] ISSN:1365-2141
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BCL2/IGH rearrangements were analysed by polymerase chain reaction (PCR) at diagnosis in paired peripheral blood (PB) and bone marrow (BM) samples from 67 patients with stage I/II follicular lymphoma (FL). Real time quantitative PCR (RQ-PCR) and digital droplet PCR (ddPCR) were performed in cases with a major breakpoint region (MBR+) at diagnosis and after localized radiotherapy and rituximab administration in order to investigate the applicability of ddPCR. The overall ddPCR/RQ-PCR concordance was 81·9% (113/138 samples) and 97·5% in the 40/138 with quantifiable disease (RQ-PCR≥10 ). At baseline, ddPCR allowed the recovery of a MBR+ marker in 8/18 (44·4%) samples that resulted MBR-negative/minor cluster region-negative/minor BCL2-negative by qualitative PCR. Moreover, the tumour burden at diagnosis significantly predicted progression-free survival (PSF) only when quantified by ddPCR. Paired PB and BM samples analysis demonstrated a high concordance in the detection of BCL2/IGH+ cells by qualitative and quantitative methods; in particular, 40/62 samples were positive by ddPCR (25 PB+/BM+; 9 PB+/BM-; 6 PB-/BM+), with 34/40 (85%) identified by the study of PB only. In conclusion, in localized FL, ddPCR is a promising tool for monitoring minimal residual disease (MRD) that is at least comparable to RQ-PCR and potentially more accurate. PB is a suitable source for serial BCL2/IGH MRD assessments, regardless of the methodology utilized.
[Mh] Termos MeSH primário: Rearranjo Gênico de Cadeia Pesada de Linfócito B/genética
Genes de Cadeia Pesada de Imunoglobulina/genética
Genes bcl-2/genética
Linfoma Folicular/genética
[Mh] Termos MeSH secundário: Antineoplásicos/uso terapêutico
Medula Óssea/fisiologia
Terapia Combinada
Seres Humanos
Leucócitos Mononucleares/fisiologia
Linfoma Folicular/diagnóstico
Linfoma Folicular/terapia
Neoplasia Residual/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
Rituximab/uso terapêutico
Translocação Genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 4F4X42SYQ6 (Rituximab)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1111/bjh.14616


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[PMID]:28069803
[Au] Autor:Sinkora M; Sinkorova J; Stepanova K
[Ad] Endereço:Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, 54922 Novy Hradek, Czech Republic marek.sinkora@worldonline.cz.
[Ti] Título:Ig Light Chain Precedes Heavy Chain Gene Rearrangement during Development of B Cells in Swine.
[So] Source:J Immunol;198(4):1543-1552, 2017 Feb 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The current mammalian paradigm states that 1) rearrangements in the IgH locus precede those in IgL loci, 2) IgLλ genes rearrange only when IgLκ genes are consumed, and 3) the surrogate L chain is necessary for selection of productive IgH gene rearrangements. We show in swine that IgL rearrangements precede IgH gene rearrangements, resulting in the expression of naked IgL on a surface of precursor B cells. Findings also suggest that there is no dependency on the surrogate L chain, and thus the authentic IgL proteins may be used for selection of the IgH repertoire. Although rearrangement starts with IgLκ genes, it is rapidly replaced by IgLλ rearrangement. Fast replacement is characterized by occurrence of IgLλ IgLκ dual-expressing precursors in which IgLκ expression is a remnant of a previous translation. Most IgLκ B cells are then generated later, indicating that there are two waves of IgLκ synthesis in different developmental stages with IgLλ gene rearrangements in between. In the absence of stromal cells, the stepwise order of rearrangements is blocked so that IgLλ gene rearrangements predominate in early B cell development. To our knowledge, this is the first evidence that some mammals can use an inverted order of Ig loci rearrangement. Moreover, a situation in which the generation of BCR-bearing IgLκ is delayed until after IgLλ becomes the dominant isotype may help explain the extreme deviations in the IgLκ/IgLλ ratios among mammals.
[Mh] Termos MeSH primário: Linfócitos B/imunologia
Rearranjo Gênico de Cadeia Leve de Linfócito B
Cadeias Leves de Imunoglobulina/genética
[Mh] Termos MeSH secundário: Animais
Linfócitos B/fisiologia
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Genes de Imunoglobulinas
Cadeias Pesadas de Imunoglobulinas/genética
Cadeias Leves de Imunoglobulina/imunologia
Cadeias kappa de Imunoglobulina/genética
Receptores de Antígenos de Linfócitos B/genética
Receptores de Antígenos de Linfócitos B/imunologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin Heavy Chains); 0 (Immunoglobulin Light Chains); 0 (Immunoglobulin kappa-Chains); 0 (Receptors, Antigen, B-Cell)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601035


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[PMID]:27461672
[Au] Autor:Bo J; Sun L; Wang W; Ma C; Li H; Zhao Y
[Ad] Endereço:Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China.
[Ti] Título:Novel diagnostic biomarker for patients with Non-Hodgkin's Lymphoma by IgH gene rearrangement.
[So] Source:J Cancer Res Ther;12(2):903-8, 2016 Apr-Jun.
[Is] ISSN:1998-4138
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:AIM OF STUDY: Novel biomarkers for improving accuracy could be beneficial for disease monitoring and surveillance of Non-Hodgkin's lymphomas (NHL). So we explored the viability of analytical methods for identifying the rearranged immunoglobulin (Ig) H genes sequence. MATERIALS AND METHODS: Next-generation sequencing (NGS) was used to sequence deoxyribonucleic acid (DNA) extracted directly from the tumor tissues of patients with NHL, and then specific rearranged DNA fragments from plasma was detected by polymerase chain reaction (PCR). RESULTS: By parallel DNA capturing and sequencing of IgH genomic regions (IgCap), the sequence of rearranged IgH loci could be detected and precisely determined in tumor tissues of 12 patients with NHL. The circulating rearranged DNA fragments had been identified in the plasma of one patient. CONCLUSION: IgCap may be the favorable diagnostic method for patients with NHL in clinical.
[Mh] Termos MeSH primário: Biomarcadores Tumorais
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Cadeias Pesadas de Imunoglobulinas/genética
Linfoma não Hodgkin/diagnóstico
Linfoma não Hodgkin/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Biologia Computacional/métodos
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Immunoglobulin Heavy Chains)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.4103/0973-1482.157345


  7 / 1570 MEDLINE  
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[PMID]:27453470
[Au] Autor:Joyce MG; Wheatley AK; Thomas PV; Chuang GY; Soto C; Bailer RT; Druz A; Georgiev IS; Gillespie RA; Kanekiyo M; Kong WP; Leung K; Narpala SN; Prabhakaran MS; Yang ES; Zhang B; Zhang Y; Asokan M; Boyington JC; Bylund T; Darko S; Lees CR; Ransier A; Shen CH; Wang L; Whittle JR; Wu X; Yassine HM; Santos C; Matsuoka Y; Tsybovsky Y; Baxa U; Mullikin JC; Subbarao K; Douek DC; Graham BS; Koup RA; Ledgerwood JE; Roederer M; Shapiro L; Kwong PD; Mascola JR; McDermott AB; NISC Comparative Sequencing Program
[Ad] Endereço:Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
[Ti] Título:Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses.
[So] Source:Cell;166(3):609-623, 2016 Jul 28.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antibodies capable of neutralizing divergent influenza A viruses could form the basis of a universal vaccine. Here, from subjects enrolled in an H5N1 DNA/MIV-prime-boost influenza vaccine trial, we sorted hemagglutinin cross-reactive memory B cells and identified three antibody classes, each capable of neutralizing diverse subtypes of group 1 and group 2 influenza A viruses. Co-crystal structures with hemagglutinin revealed that each class utilized characteristic germline genes and convergent sequence motifs to recognize overlapping epitopes in the hemagglutinin stem. All six analyzed subjects had sequences from at least one multidonor class, and-in half the subjects-multidonor-class sequences were recovered from >40% of cross-reactive B cells. By contrast, these multidonor-class sequences were rare in published antibody datasets. Vaccination with a divergent hemagglutinin can thus increase the frequency of B cells encoding broad influenza A-neutralizing antibodies. We propose the sequence signature-quantified prevalence of these B cells as a metric to guide universal influenza A immunization strategies.
[Mh] Termos MeSH primário: Anticorpos Neutralizantes/imunologia
Anticorpos Antivirais/imunologia
Vírus da Influenza A/imunologia
Vacinas contra Influenza/imunologia
[Mh] Termos MeSH secundário: Adulto
Sequência de Aminoácidos
Anticorpos Neutralizantes/química
Anticorpos Neutralizantes/genética
Anticorpos Antivirais/química
Anticorpos Antivirais/genética
Linfócitos B/imunologia
Epitopos de Linfócito B
Feminino
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Seres Humanos
Memória Imunológica
Vírus da Influenza A Subtipo H5N1/imunologia
Masculino
Meia-Idade
Modelos Moleculares
Estrutura Terciária de Proteína
Relação Estrutura-Atividade
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE I; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Epitopes, B-Lymphocyte); 0 (Influenza Vaccines)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170910
[Lr] Data última revisão:
170910
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE


  8 / 1570 MEDLINE  
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[PMID]:27445561
[Au] Autor:Li P; Cheung L; Chiu B
[Ad] Endereço:Department of Medicine, University of Alberta, Edmonton, AB, Canada T6G 2G3.
[Ti] Título:Early Bronchus-Associated Lymphoid Tissue Lymphoma Diagnosed with Immunoglobulin Heavy Chain Molecular Testing.
[So] Source:Can Respir J;2016:7056035, 2016.
[Is] ISSN:1916-7245
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:When extranodal marginal zone B-cell lymphoma of mucosa associated lymphoid tissue (MALT), a low grade B-cell lymphoma, arises in the lung it is referred to as bronchus-associated lymphoid tissue (BALT) lymphoma. We describe a patient with a history of Sjögren's syndrome and rheumatoid arthritis with dyspnea and imaging consistent with lymphoid interstitial pneumonia (LIP). However, while histology and immunohistochemistry lacked definitive features of a lymphoma, immunoglobulin heavy chain (IgH) polymerase chain reaction testing demonstrated B-cell monoclonality, consistent with an early BALT lymphoma.
[Mh] Termos MeSH primário: Neoplasias Brônquicas/diagnóstico por imagem
Linfoma de Zona Marginal Tipo Células B/diagnóstico por imagem
[Mh] Termos MeSH secundário: Idoso
Artrite Reumatoide/complicações
Artrite Reumatoide/tratamento farmacológico
Neoplasias Brônquicas/complicações
Neoplasias Brônquicas/genética
Neoplasias Brônquicas/patologia
Tosse/etiologia
Dispneia/etiologia
Feminino
Rearranjo Gênico de Cadeia Pesada de Linfócito B/genética
Seres Humanos
Cadeias Pesadas de Imunoglobulinas/genética
Infliximab/uso terapêutico
Linfoma de Zona Marginal Tipo Células B/complicações
Linfoma de Zona Marginal Tipo Células B/genética
Linfoma de Zona Marginal Tipo Células B/patologia
Reação em Cadeia da Polimerase
Síndrome de Sjogren/complicações
Síndrome de Sjogren/tratamento farmacológico
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin Heavy Chains); B72HH48FLU (Infliximab)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE
[do] DOI:10.1155/2016/7056035


  9 / 1570 MEDLINE  
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[PMID]:27352258
[Au] Autor:Kiaei A; Onsori H; Alijani A; Andalib S; Ghorbian S; Sakhinia E
[Ad] Endereço:Department of Biology, Tabriz Branch, Islamic Azad University Tabriz, Iran; Department of Biology, College of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
[Ti] Título:Detection of t(8;14) c-myc/IgH gene rearrangement by long-distance polymerase chain reaction in patients with diffuse large B-cell lymphoma.
[So] Source:Hematol Oncol Stem Cell Ther;9(4):141-146, 2016 Dec.
[Is] ISSN:1658-3876
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE/BACKGROUND: Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL. METHODS: In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and γ switch region) and the myc gene (Exons 2 and 3). RESULTS: As much as 1.85% of the samples were positive for the γ constant region and Exon 2 of the myc gene. CONCLUSION: LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL.
[Mh] Termos MeSH primário: Cromossomos Humanos Par 14/genética
Cromossomos Humanos Par 8/genética
Rearranjo Gênico de Cadeia Pesada de Linfócito B/genética
Linfoma Difuso de Grandes Células B/genética
Reação em Cadeia da Polimerase/métodos
Proteínas Proto-Oncogênicas c-myc/genética
Translocação Genética/genética
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Quebra Cromossômica
Eletroforese em Gel de Ágar
Etídio/metabolismo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proto-Oncogene Proteins c-myc); EN464416SI (Ethidium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE


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[PMID]:27211983
[Au] Autor:Muñoz-Largacha JA; O'Hara CJ; Sloan JM; Fernando HC; Litle VR
[Ad] Endereço:Department of Surgery, Division of Thoracic Surgery, Boston University School of Medicine, Boston, Massachusetts.
[Ti] Título:Surgical Management of Pulmonary Mucosa-Associated Lymphoid Tissue Lymphoma Associated With Light-Chain Deposition Disease.
[So] Source:Ann Thorac Surg;101(6):e207-10, 2016 Jun.
[Is] ISSN:1552-6259
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A 52-year-old woman presented with a right middle lobe (RML) lung nodule suspicious for malignancy. Thoracoscopic middle lobectomy was performed. The pathology report revealed a pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma in association with light-chain deposition disease (LCDD). Pulmonary MALT lymphoma and LCDD are unusual disorders presenting in the lung, and the association between these 2 conditions is even more uncommon. The optimal management for these patients is controversial, although surgical resection of localized well-circumscribed lesions may represent an effective therapeutic approach.
[Mh] Termos MeSH primário: Cadeias kappa de Imunoglobulina
Neoplasias Pulmonares/cirurgia
Linfoma de Zona Marginal Tipo Células B/cirurgia
Gamopatia Monoclonal de Significância Indeterminada/complicações
Pneumonectomia/métodos
[Mh] Termos MeSH secundário: Amiloidose/diagnóstico
Linfócitos B/patologia
Células Clonais/patologia
Vermelho Congo
Diagnóstico Diferencial
Feminino
Rearranjo Gênico de Cadeia Pesada de Linfócito B
Seres Humanos
Cadeias kappa de Imunoglobulina/sangue
Neoplasias Pulmonares/complicações
Neoplasias Pulmonares/diagnóstico por imagem
Linfoma de Zona Marginal Tipo Células B/complicações
Linfoma de Zona Marginal Tipo Células B/diagnóstico por imagem
Meia-Idade
Gamopatia Monoclonal de Significância Indeterminada/diagnóstico
Gamopatia Monoclonal de Significância Indeterminada/patologia
Coloração e Rotulagem
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin kappa-Chains); 3U05FHG59S (Congo Red)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160524
[St] Status:MEDLINE



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