Base de dados : MEDLINE
Pesquisa : G05.360 [Categoria DeCS]
Referências encontradas : 277 [refinar]
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[PMID]:29208645
[Au] Autor:Mariezcurrena A; Uhlmann F
[Ad] Endereço:Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, United Kingdom.
[Ti] Título:Observation of DNA intertwining along authentic budding yeast chromosomes.
[So] Source:Genes Dev;31(21):2151-2161, 2017 11 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA replication of circular genomes generates physically interlinked or catenated sister DNAs. These are resolved through transient DNA fracture by type II topoisomerases to permit chromosome segregation during cell division. Topoisomerase II is similarly required for linear chromosome segregation, suggesting that linear chromosomes also remain intertwined following DNA replication. Indeed, chromosome resolution defects are a frequent cause of chromosome segregation failure and consequent aneuploidies. When and where intertwines arise and persist along linear chromosomes are not known, owing to the difficulty of demonstrating intertwining of linear DNAs. Here, we used excision of chromosomal regions as circular "loop outs" to convert sister chromatid intertwines into catenated circles. This revealed intertwining at replication termination and cohesin-binding sites, where intertwines are thought to arise and persist but not to a greater extent than elsewhere in the genome. Intertwining appears to spread evenly along chromosomes but is excluded from heterochromatin. We found that intertwines arise before replication termination, suggesting that replication forks rotate during replication elongation to dissipate torsion ahead of the forks. Our approach provides previously inaccessible insight into the topology of eukaryotic chromosomes and illuminates a process critical for successful chromosome segregation.
[Mh] Termos MeSH primário: Cromossomos Fúngicos/metabolismo
Replicação do DNA
DNA Fúngico/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Segregação de Cromossomos
Estruturas Genéticas
Genoma Fúngico
Heterocromatina/metabolismo
Origem de Replicação/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (DNA, Fungal); 0 (Heterochromatin); 0 (cohesins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1101/gad.305557.117


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[PMID]:28804138
[Au] Autor:Eilbeck K; Quinlan A; Yandell M
[Ad] Endereço:Department of Biomedical Informatics, School of Medicine, University of Utah, 421 Wakara Way, Suite 120, Salt Lake City, Utah 84108, USA.
[Ti] Título:Settling the score: variant prioritization and Mendelian disease.
[So] Source:Nat Rev Genet;18(10):599-612, 2017 Oct.
[Is] ISSN:1471-0064
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:When investigating Mendelian disease using exome or genome sequencing, distinguishing disease-causing genetic variants from the multitude of candidate variants is a complex, multidimensional task. Many prioritization tools and online interpretation resources exist, and professional organizations have offered clinical guidelines for review and return of prioritization results. In this Review, we describe the strengths and weaknesses of widely used computational approaches, explain their roles in the diagnostic and discovery process and discuss how they can inform (and misinform) expert reviewers. We place variant prioritization in the wider context of gene prioritization, burden testing and genotype-phenotype association, and we discuss opportunities and challenges introduced by whole-genome sequencing.
[Mh] Termos MeSH primário: Doença/genética
Variação Genética
[Mh] Termos MeSH secundário: Variações do Número de Cópias de DNA
Estruturas Genéticas
Genoma Humano
Estudo de Associação Genômica Ampla
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1038/nrg.2017.52


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[PMID]:28800596
[Au] Autor:Sun S; Yadav V; Billmyre RB; Cuomo CA; Nowrousian M; Wang L; Souciet JL; Boekhout T; Porcel B; Wincker P; Granek JA; Sanyal K; Heitman J
[Ad] Endereço:Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America.
[Ti] Título:Fungal genome and mating system transitions facilitated by chromosomal translocations involving intercentromeric recombination.
[So] Source:PLoS Biol;15(8):e2002527, 2017 Aug.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Species within the human pathogenic Cryptococcus species complex are major threats to public health, causing approximately 1 million annual infections globally. Cryptococcus amylolentus is the most closely known related species of the pathogenic Cryptococcus species complex, and it is non-pathogenic. Additionally, while pathogenic Cryptococcus species have bipolar mating systems with a single large mating type (MAT) locus that represents a derived state in Basidiomycetes, C. amylolentus has a tetrapolar mating system with 2 MAT loci (P/R and HD) located on different chromosomes. Thus, studying C. amylolentus will shed light on the transition from tetrapolar to bipolar mating systems in the pathogenic Cryptococcus species, as well as its possible link with the origin and evolution of pathogenesis. In this study, we sequenced, assembled, and annotated the genomes of 2 C. amylolentus isolates, CBS6039 and CBS6273, which are sexual and interfertile. Genome comparison between the 2 C. amylolentus isolates identified the boundaries and the complete gene contents of the P/R and HD MAT loci. Bioinformatic and chromatin immunoprecipitation sequencing (ChIP-seq) analyses revealed that, similar to those of the pathogenic Cryptococcus species, C. amylolentus has regional centromeres (CENs) that are enriched with species-specific transposable and repetitive DNA elements. Additionally, we found that while neither the P/R nor the HD locus is physically closely linked to its centromere in C. amylolentus, and the regions between the MAT loci and their respective centromeres show overall synteny between the 2 genomes, both MAT loci exhibit genetic linkage to their respective centromere during meiosis, suggesting the presence of recombinational suppressors and/or epistatic gene interactions in the MAT-CEN intervening regions. Furthermore, genomic comparisons between C. amylolentus and related pathogenic Cryptococcus species provide evidence that multiple chromosomal rearrangements mediated by intercentromeric recombination have occurred during descent of the 2 lineages from their common ancestor. Taken together, our findings support a model in which the evolution of the bipolar mating system was initiated by an ectopic recombination event mediated by similar repetitive centromeric DNA elements shared between chromosomes. This translocation brought the P/R and HD loci onto the same chromosome, and further chromosomal rearrangements then resulted in the 2 MAT loci becoming physically linked and eventually fusing to form the single contiguous MAT locus that is now extant in the pathogenic Cryptococcus species.
[Mh] Termos MeSH primário: Cryptococcus/citologia
Cryptococcus/genética
Genes Fúngicos Tipo Acasalamento
Genoma Fúngico
Meiose
Translocação Genética
[Mh] Termos MeSH secundário: Imunoprecipitação da Cromatina
Biologia Computacional
Troca Genética
Cryptococcus/crescimento & desenvolvimento
Cryptococcus/fisiologia
Cryptococcus neoformans/citologia
Cryptococcus neoformans/genética
Cryptococcus neoformans/fisiologia
Epistasia Genética
Evolução Molecular
Ligação Genética
Loci Gênicos
Estruturas Genéticas
Desequilíbrio de Ligação
Anotação de Sequência Molecular
Recombinação Genética
Análise de Sequência de RNA
Especificidade da Espécie
Sintenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2002527


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[PMID]:28760881
[Au] Autor:Iacoangeli A; Lui A; Haines A; Ohta Y; Flajnik M; Hsu E
[Ad] Endereço:Tisch Multiple Sclerosis Research Center of New York, New York, NY 10019.
[Ti] Título:Evidence for Ig Light Chain Isotype Exclusion in Shark B Lymphocytes Suggests Ordered Mechanisms.
[So] Source:J Immunol;199(5):1875-1885, 2017 Sep 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Unlike most vertebrates, the shark IgL gene organization precludes secondary rearrangements that delete self-reactive VJ rearranged genes. Nurse sharks express four L chain isotypes, κ, λ, σ, and σ-2, encoded by 35 functional minigenes or clusters. The sequence of gene activation/expression and receptor editing of these isotypes have not been studied. We therefore investigated the extent of isotypic exclusion in separated B cell subpopulations. Surface Ig (sIg)κ-expressing cells, isolated with mAb LK14 that recognizes Cκ, carry predominantly nonproductive rearrangements of other L chain isotypes. Conversely, after depletion with LK14, sIgM cells contained largely nonproductive κ and enrichment for in-frame VJ of the others. Because some isotypic inclusion was observed at the mRNA level, expression in the BCR was examined. Functional λ mRNA was obtained, as expected, from the LK14-depleted population, but was also in sIgκ splenocytes. Whereas λ somatic mutants from the depleted sample displayed evidence of positive selection, the λ genes in sIgκ cells accumulated bystander mutations indicating a failure to express their products at the cell surface in association with the BCR H chain. In conclusion, a shark B cell expresses one L chain isotype at the surface and other isotypes as nonproductive VJ, sterile transcripts, or in-frame VJ whose products may not associate with the H chain. Based on the mRNA content found in the B cell subpopulations, an order of L chain gene activation is suggested as: σ-2 followed by κ, then σ and λ.
[Mh] Termos MeSH primário: Subpopulações de Linfócitos B/fisiologia
Linfócitos B/fisiologia
Proteínas de Peixes/genética
Rearranjo Gênico de Cadeia Leve de Linfócito B
Isotipos de Imunoglobulinas/genética
Cadeias Leves de Imunoglobulina/genética
Receptores de Antígenos de Linfócitos B/genética
Tubarões/imunologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Feminino
Regulação da Expressão Gênica
Loci Gênicos
Estruturas Genéticas
Switching de Imunoglobulina
Masculino
RNA Mensageiro
Vertebrados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Immunoglobulin Isotypes); 0 (Immunoglobulin Light Chains); 0 (RNA, Messenger); 0 (Receptors, Antigen, B-Cell)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700762


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[PMID]:28131766
[Au] Autor:Thulasitha WS; Umasuthan N; Wan Q; Nam BH; Kang TW; Lee J
[Ad] Endereço:Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea; Department of Zoology, University
[Ti] Título:A proto-type galectin-2 from rock bream (Oplegnathus fasciatus): Molecular, genomic, and expression analysis, and recognition of microbial pathogens by recombinant protein.
[So] Source:Dev Comp Immunol;71:70-81, 2017 06.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A ß-galactoside binding lectin, designated as galectin-2, was identified and characterized from rock bream Oplegnathus fasciatus (OfGal-2). The cDNA of OfGal-2 comprised of 692 bp with a coding sequence of 396 bp, encoding a putative polypeptide of 131 amino acids. Gene structure analysis of OfGal-2 revealed a four exon-three intron organization. A single carbohydrate-binding domain containing all seven important residues for carbohydrate binding was located in the third exon, which formed a carbohydrate-binding pocket. Homology screening and sequence analysis demonstrated that OfGal-2 is an evolutionarily conserved proto-type galectin. OfGal-2 transcripts were detected in several healthy fish tissues, with the highest level observed in the intestine, followed by the liver. The expression of OfGal-2 was elevated upon the injection of various mitogenic stimulants and pathogens in a time-dependent manner. Upregulated expression in the liver after tissue injury suggested its role as a damage-associated molecular pattern. Recombinant OfGal-2 protein had hemagglutinating potential and possessed affinity towards lactose and galactose. Moreover, the recombinant protein agglutinated and bound potential pathogenic bacteria and a ciliate. The results of this study indicate that the galectin-2 from rock bream has a potential role in immunity, particularly in the recognition of invading pathogens.
[Mh] Termos MeSH primário: Infecções Bacterianas/imunologia
Proteínas de Peixes/metabolismo
Galectina 2/metabolismo
Intestinos/fisiologia
Tecido Linfoide/fisiologia
Perciformes/imunologia
Receptores de Reconhecimento de Padrão/metabolismo
Proteínas Recombinantes/metabolismo
Viroses/imunologia
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Proteínas de Peixes/genética
Galectina 2/genética
Regulação da Expressão Gênica
Estruturas Genéticas
Tecido Linfoide/microbiologia
Tecido Linfoide/virologia
Filogenia
Receptores de Reconhecimento de Padrão/genética
Proteínas Recombinantes/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Galectin 2); 0 (Receptors, Pattern Recognition); 0 (Recombinant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170130
[St] Status:MEDLINE


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[PMID]:28111231
[Au] Autor:Yang Z; Sun J; Ji H; Shi XC; Li Y; Du ZY; Chen LQ
[Ad] Endereço:College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
[Ti] Título:Pigment epithelium-derived factor improves TNFα-induced hepatic steatosis in grass carp (Ctenopharyngodon idella).
[So] Source:Dev Comp Immunol;71:8-17, 2017 06.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNFα), may contribute to hepatic steatosis in the situation of excess lipid accumulation in farmed fish. Pigment epithelium-derived factor (PEDF) is an endogenous anti-inflammatory factor and promotes lipolysis. Accordingly, we isolated PEDF from grass carp and investigated its role in TNFα-induced hepatic steatosis. Sequence analysis showed that PEDF gene, which possesses 8 exons and 7 introns, encodes a protein with 409 amino acids. PEDF was a critical determinant of the transcriptional response to nutrient availability in grass carp. Endogenous PEDF was an intracellular protein with cytoplasmic distribution and directly interacts with adipose triglyceride lipase (ATGL), which might mediate PEDF-induced lipolysis. TNFα significantly promoted lipid accumulation in vivo and in vitro, accompanied with a decrease in mRNA levels of PEDF and peroxisome proliferator-activated receptor alpha (PPARα). Recombinant PEDF and PPARα agonist diminished the TNFα-induced hepatic steatosis. Meanwhile, PPARα agonist caused an increase in PEDF expression, suggesting that TNFα antagonizes the actions of PEDF possibly in a PPARα-dependent manner. These findings suggest that PEDF is an important protective factor against hepatic steatosis induced by TNFα, which provided a new therapeutic target for inflammation-associated hepatic steatosis.
[Mh] Termos MeSH primário: Carpas/imunologia
Proteínas do Olho/metabolismo
Fígado Gorduroso/imunologia
Doenças dos Peixes/imunologia
Proteínas de Peixes/metabolismo
Inflamação/imunologia
Fatores de Crescimento Neural/metabolismo
Epitélio Pigmentado Ocular/metabolismo
Serpinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Proteínas do Olho/genética
Proteínas de Peixes/genética
Regulação da Expressão Gênica
Estruturas Genéticas
Lipase/metabolismo
Lipólise
Fatores de Crescimento Neural/genética
PPAR alfa/metabolismo
Serpinas/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Eye Proteins); 0 (Fish Proteins); 0 (Nerve Growth Factors); 0 (PPAR alpha); 0 (Serpins); 0 (Tumor Necrosis Factor-alpha); 0 (pigment epithelium-derived factor); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


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[PMID]:28111230
[Au] Autor:Huang B; Meng J; Yang M; Xu F; Li X; Li L; Zhang G
[Ad] Endereço:Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; National and Local Joint Engineering Laboratory of Ecol
[Ti] Título:Characterization of the IRF2 proteins isolated from the deep-sea mussel Bathymodiolus platifrons and the shallow-water mussel Modiolus modiolus.
[So] Source:Dev Comp Immunol;71:82-87, 2017 06.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interferon regulatory factors (IRFs) are transcription factors that play important roles in immune defense, stress response, hematopoietic differentiation, and cell apoptosis. IRFs of invertebrate organisms and their functions remain largely unexplored. In the present study, for the first time new IRFs (BpIRF2 and MmIRF2) were identified in the deep-sea mussel Bathymodiolus platifrons and the shallow-water mussel Modiolus modiolus. The open reading frame of BpIRF2 and MmIRF2 encoded putative proteins of 354 and 348 amino acids, respectively. Comparison and phylogenetic analysis revealed that both IRF2 proteins were new identified invertebrate IRF molecular. As transcriptional factors, both BpIRF2 and MmIRF2 could activate the interferon-stimulated response element-containing promoter and BpIRF2 could interact with itself. Moreover, both BpIRF2 and MmIRF2 were localized to the cytoplasm and nucleus. Collectively, these results demonstrated that IRF2 proteins might be crucial in the innate immunity of deep-sea and shallow-water mussels.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Citoplasma/metabolismo
Fator Regulador 2 de Interferon/metabolismo
Mytilidae/imunologia
Ativação Transcricional
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Estruturas Genéticas
Células HeLa
Seres Humanos
Imunidade Inata/genética
Fator Regulador 2 de Interferon/genética
Filogenia
Regiões Promotoras Genéticas/genética
Ligação Proteica
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Interferon Regulatory Factor-2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


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[PMID]:27838627
[Au] Autor:Montinaro F; Busby GB; Gonzalez-Santos M; Oosthuitzen O; Oosthuitzen E; Anagnostou P; Destro-Bisol G; Pascali VL; Capelli C
[Ad] Endereço:Department of Zoology, University of Oxford, OX1 3PS, UK francesco.montinaro@gmail.com.
[Ti] Título:Complex Ancient Genetic Structure and Cultural Transitions in Southern African Populations.
[So] Source:Genetics;205(1):303-316, 2017 Jan.
[Is] ISSN:1943-2631
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The characterization of the structure of southern African populations has been the subject of numerous genetic, medical, linguistic, archaeological, and anthropological investigations. Current diversity in the subcontinent is the result of complex events of genetic admixture and cultural contact between early inhabitants and migrants that arrived in the region over the last 2000 years. Here, we analyze 1856 individuals from 91 populations, comprising novel and published genotype data, to characterize the genetic ancestry profiles of 631 individuals from 51 southern African populations. Combining both local ancestry and allele frequency based analyses, we identify a tripartite, ancient, Khoesan-related genetic structure. This structure correlates neither with linguistic affiliation nor subsistence strategy, but with geography, revealing the importance of isolation-by-distance dynamics in the area. Fine-mapping of these components in southern African populations reveals admixture and cultural reversion involving several Khoesan groups, and highlights that Bantu speakers and Coloured individuals have different mixtures of these ancient ancestries.
[Mh] Termos MeSH primário: Grupo com Ancestrais do Continente Africano/genética
DNA Antigo/análise
Estruturas Genéticas
[Mh] Termos MeSH secundário: África Austral
DNA Antigo/química
Frequência do Gene
Variação Genética
Genética Populacional/métodos
Genótipo
Haplótipos
Seres Humanos
Lesoto
Namíbia
Filogeografia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ancient)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161114
[St] Status:MEDLINE
[do] DOI:10.1534/genetics.116.189209


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[PMID]:26947590
[Au] Autor:Wang D; Huang X; Chen J; Mou Y; Qi Y
[Ad] Endereço:Department of Clinical Laboratories for Infectious Disease, Taizhou Municipal Hospital, Zhejiang, China; Institute of Molecular Diagnostics of Taizhou University, Zhejiang, China. Electronic address: wdgtzs@163.com.
[Ti] Título:Characterization of a novel qepA3 variant in Enterobacter aerogenes.
[So] Source:J Microbiol Immunol Infect;50(2):254-257, 2017 Apr.
[Is] ISSN:1995-9133
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Five isolates harboring qepA were studied by polymerase chain reaction (PCR) amplification and relevant methods. One was determined to be a novel qepA3 from Enterobacter aerogenes, and four involved three qepA1 and one qepA2 determinants from Escherichia coli; the qepA3 changed five amino acids. These results characterized genetic structures A, B, C, D, and E.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Enterobacter aerogenes/genética
[Mh] Termos MeSH secundário: Anti-Infecciosos/farmacologia
DNA Bacteriano/genética
Farmacorresistência Bacteriana
Enterobacter aerogenes/efeitos dos fármacos
Enterobacter cloacae/efeitos dos fármacos
Enterobacter cloacae/genética
Infecções por Enterobacteriaceae/microbiologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Genes Bacterianos
Estruturas Genéticas
Testes de Sensibilidade Microbiana
Mutação Puntual
Reação em Cadeia da Polimerase/métodos
Quinolonas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Quinolones)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE


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[PMID]:27965242
[Au] Autor:Baute GJ; Owens GL; Bock DG; Rieseberg LH
[Ad] Endereço:Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada.
[Ti] Título:Genome-wide genotyping-by-sequencing data provide a high-resolution view of wild Helianthus diversity, genetic structure, and interspecies gene flow.
[So] Source:Am J Bot;103(12):2170-2177, 2016 Dec.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE: Wild sunflowers harbor considerable genetic diversity and are a major resource for improvement of the cultivated sunflower, Helianthus annuus. The Helianthus genus is also well known for its propensity for gene flow between taxa. METHODS: We surveyed genomic diversity of 292 samples of wild Helianthus from 22 taxa that are cross-compatible with the cultivar using genotyping by sequencing. With these data, we derived a high-resolution phylogeny of the taxa, interrogated genome-wide levels of diversity, explored H. annuus population structure, and identified localized gene flow between H. annuus and its close relatives. KEY RESULTS: Our phylogenomic analyses confirmed a number of previously established interspecific relationships and indicated for the first time that a newly described annual sunflower, H. winteri, is nested within H. annuus. Principal component analyses showed that H. annuus has geographic population structure with most notable subpopulations occurring in California and Texas. While gene flow was identified between H. annuus and H. bolanderi in California and between H. annuus and H. argophyllus in Texas, this genetic exchange does not appear to drive observed patterns of H. annuus population structure. CONCLUSIONS: Wild H. annuus remains an excellent resource for cultivated sunflower breeding effort because of its diversity and the ease with which it can be crossed with cultivated H. annuus. Cases of interspecific gene flow such as those documented here also indicate wild H. annuus can act as a bridge to capture alleles from other wild taxa; continued breeding efforts with it may therefore reap the largest rewards.
[Mh] Termos MeSH primário: Fluxo Gênico
Variação Genética
Genoma de Planta/genética
Genômica
Helianthus/genética
[Mh] Termos MeSH secundário: Alelos
Cruzamento
Demografia
Estruturas Genéticas
Genótipo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600295



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