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Pesquisa : G05.360.337.500 [Categoria DeCS]
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  1 / 3119 MEDLINE  
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[PMID]:27577857
[Au] Autor:Kudo F; Tsunoda T; Takashima M; Eguchi T
[Ad] Endereço:Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-Okayama, Meguro-ku, Tokyo, 152-8551 (, Japan).
[Ti] Título:Five-Membered Cyclitol Phosphate Formation by a myo-Inositol Phosphate Synthase Orthologue in the Biosynthesis of the Carbocyclic Nucleoside Antibiotic Aristeromycin.
[So] Source:Chembiochem;17(22):2143-2148, 2016 Nov 17.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Aristeromycin is a unique carbocyclic nucleoside antibiotic produced by Streptomyces citricolor. In order to elucidate its intriguing carbocyclic formation, we used a genome-mining approach to identify the responsible enzyme. In silico screening with known cyclitol synthases involved in primary metabolism, such as myo-inositol-1-phosphate synthase (MIPS) and dehydroqunate synthase (DHQS), identified a unique MIPS orthologue (Ari2) encoded in the genome of S. citricolor. Heterologous expression of the gene cluster containing ari2 with a cosmid vector in Streptomyces albus resulted in the production of aristeromycin, thus indicating that the cloned DNA region (37.5 kb) with 33 open reading frames contains its biosynthetic gene cluster. We verified that Ari2 catalyzes the formation of a novel five-membered cyclitol phosphate from d-fructose 6-phosphate (F6P) with NAD as a cofactor. This provides insight into cyclitol phosphate synthase as a member of the MIPS family of enzymes. A biosynthetic pathway to aristeromycin is proposed based on bioinformatics analysis of the gene cluster.
[Mh] Termos MeSH primário: Adenosina/análogos & derivados
Antibacterianos/biossíntese
Proteínas de Bactérias/metabolismo
Ciclitóis/metabolismo
Mio-Inositol-1-Fosfato Sintase/metabolismo
Fósforo-Oxigênio Liases/metabolismo
[Mh] Termos MeSH secundário: Adenosina/biossíntese
Adenosina/química
Antibacterianos/química
Proteínas de Bactérias/genética
Cosmídeos/genética
Cosmídeos/metabolismo
Ciclitóis/química
Espectroscopia de Ressonância Magnética
Família Multigênica
Mio-Inositol-1-Fosfato Sintase/genética
Nucleosídeos/química
Fósforo-Oxigênio Liases/genética
Espectrometria de Massas por Ionização por Electrospray
Streptomyces coelicolor/enzimologia
Streptomyces coelicolor/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Cyclitols); 0 (Nucleosides); 19186-33-5 (aristeromycin); EC 4.2.3.4 (3-dehydroquinate synthetase); EC 4.6.- (Phosphorus-Oxygen Lyases); EC 5.5.1.4 (Myo-Inositol-1-Phosphate Synthase); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201600348


  2 / 3119 MEDLINE  
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[PMID]:27162331
[Au] Autor:Gazanion É; Fernández-Prada C; Papadopoulou B; Leprohon P; Ouellette M
[Ad] Endereço:Centre de Recherche en Infectiologie du Centre de Recherche du Centre Hospitalier Universitaire de Québec, Université Laval, Québec, QC, Canada G1V 4G2; Département de Microbiologie, Infectiologie, et Immunologie, Faculté de Médecine, Université Laval, Québec, QC, Canada G1V 0A6.
[Ti] Título:Cos-Seq for high-throughput identification of drug target and resistance mechanisms in the protozoan parasite Leishmania.
[So] Source:Proc Natl Acad Sci U S A;113(21):E3012-21, 2016 May 24.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Innovative strategies are needed to accelerate the identification of antimicrobial drug targets and resistance mechanisms. Here we develop a sensitive method, which we term Cosmid Sequencing (or "Cos-Seq"), based on functional cloning coupled to next-generation sequencing. Cos-Seq identified >60 loci in the Leishmania genome that were enriched via drug selection with methotrexate and five major antileishmanials (antimony, miltefosine, paromomycin, amphotericin B, and pentamidine). Functional validation highlighted both known and previously unidentified drug targets and resistance genes, including novel roles for phosphatases in resistance to methotrexate and antimony, for ergosterol and phospholipid metabolism genes in resistance to miltefosine, and for hypothetical proteins in resistance to paromomycin, amphothericin B, and pentamidine. Several genes/loci were also found to confer resistance to two or more antileishmanials. This screening method will expedite the discovery of drug targets and resistance mechanisms and is easily adaptable to other microorganisms.
[Mh] Termos MeSH primário: Resistência a Medicamentos/genética
Genes de Protozoários
Sequenciamento de Nucleotídeos em Larga Escala
Leishmania infantum/genética
[Mh] Termos MeSH secundário: Antiprotozoários/farmacologia
Cosmídeos/genética
Resistência a Medicamentos/efeitos dos fármacos
Fosfolipídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Phospholipids)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1520693113


  3 / 3119 MEDLINE  
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[PMID]:25963315
[Au] Autor:Mendez GS; Delwiche CF; Apt KE; Lippmeier JC
[Ad] Endereço:Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, Maryland, 20742-5815.
[Ti] Título:Dinoflagellate Gene Structure and Intron Splice Sites in a Genomic Tandem Array.
[So] Source:J Eukaryot Microbiol;62(5):679-87, 2015 Sep-Oct.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dinoflagellates are one of the last major lineages of eukaryotes for which little is known about genome structure and organization. We report here the sequence and gene structure of a clone isolated from a cosmid library which, to our knowledge, represents the largest contiguously sequenced, dinoflagellate genomic, tandem gene array. These data, combined with information from a large transcriptomic library, allowed a high level of confidence of every base pair call. This degree of confidence is not possible with PCR-based contigs. The sequence contains an intron-rich set of five highly expressed gene repeats arranged in tandem. One of the tandem repeat gene members contains an intron 26,372 bp long. This study characterizes a splice site consensus sequence for dinoflagellate introns. Two to nine base pairs around the 3' splice site are repeated by an identical two to nine base pairs around the 5' splice site. The 5' and 3' splice sites are in the same locations within each repeat so that the repeat is found only once in the mature mRNA. This identically repeated intron boundary sequence might be useful in gene modeling and annotation of genomes.
[Mh] Termos MeSH primário: Dinoflagelados/genética
Genoma de Protozoário
Genômica/métodos
Íntrons
Processamento de RNA
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Cosmídeos
Biblioteca Gênica
Genoma de Protozoário/genética
Dados de Sequência Molecular
Sequências de Repetição em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150513
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12230


  4 / 3119 MEDLINE  
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[PMID]:25934225
[Au] Autor:Arai M; Kamiya K; Pruksakorn P; Sumii Y; Kotoku N; Joubert JP; Moodley P; Han C; Shin D; Kobayashi M
[Ad] Endereço:Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka 1-6, Suita, Osaka 565-0871, Japan. Electronic address: araim@phs.osaka-u.ac.jp.
[Ti] Título:Anti-dormant mycobacterial activity and target analysis of nybomycin produced by a marine-derived Streptomyces sp.
[So] Source:Bioorg Med Chem;23(13):3534-41, 2015 Jul 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the course of our search for anti-dormant Mycobacterial substances, nybomycin (1) was re-discovered from the culture broth of a marine-derived Streptomyces sp. on the bioassay-guided separation. Compound 1 showed anti-microbial activity against Mycobacterium smegmatis and Mycobacterium bovis BCG with the MIC of 1.0µg/mL under both actively growing aerobic conditions and dormancy inducing hypoxic conditions. Compound 1 is also effective to Mycobacterium tuberculosis including the clinically isolated strains. The mechanistic analysis indicated that 1 bound to DNA and induces a unique morphological change to mycobacterial bacilli leading the bacterial cell death.
[Mh] Termos MeSH primário: Antituberculosos/farmacologia
DNA Bacteriano/antagonistas & inibidores
Mycobacterium tuberculosis/efeitos dos fármacos
Streptomyces/química
[Mh] Termos MeSH secundário: Antituberculosos/química
Antituberculosos/isolamento & purificação
Organismos Aquáticos
Técnicas de Cultura de Células
Cosmídeos/química
Cosmídeos/metabolismo
DNA Bacteriano/química
Farmacorresistência Bacteriana/genética
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Mycobacterium bovis/efeitos dos fármacos
Mycobacterium bovis/genética
Mycobacterium bovis/metabolismo
Mycobacterium bovis/ultraestrutura
Mycobacterium smegmatis/efeitos dos fármacos
Mycobacterium smegmatis/genética
Mycobacterium smegmatis/metabolismo
Mycobacterium smegmatis/ultraestrutura
Mycobacterium tuberculosis/genética
Mycobacterium tuberculosis/metabolismo
Mycobacterium tuberculosis/ultraestrutura
Quinolonas/química
Quinolonas/isolamento & purificação
Quinolonas/farmacologia
Streptomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (DNA, Bacterial); 0 (Quinolones); NLB430F005 (nybomycin)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150608
[Lr] Data última revisão:
150608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150503
[St] Status:MEDLINE


  5 / 3119 MEDLINE  
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[PMID]:25341397
[Au] Autor:Wang J; Zhu Y; Zhao G; Zhu J; Wu S
[Ad] Endereço:State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang, Beijing, 100101, People's Republic of China.
[Ti] Título:Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain.
[So] Source:Appl Microbiol Biotechnol;99(7):3069-80, 2015 Apr.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A two-step method, i.e., the transfer acyl analysis and then the chiral HPLC analysis, was employed in the screening of the cosmid library of Microbacterium hydrocarbonoxydans genome. Two enantiocomplementary γ-lactamase clones were found. A 40-kb cosmid showed (-)-γ-lactamase activity, and the activity was from Mhg which was reported previously according to the results of PCR identifying experiment. The 37-kb (+)-γ-lactamase cosmid was further constructed into a pUC18 plasmid library and screened by the same two-step method. A plasmid clone harboring a 1.6-kb fragment showed (+)-γ-lactamase activity. A 555-bp ORF in the 1.6-kb fragment showed high (+)-γ-lactamase activity when it was expressed under the control of T7 promoter. The coding protein showed significant homology with bacterial isochorismatase. The (+)-γ-lactamase was characterized and compared with the (-)-γ-lactamase Mhg. This is another report that two enantiocomplementary γ-lactamases are present in the same strain.
[Mh] Termos MeSH primário: Actinomycetales/enzimologia
Amidoidrolases/genética
Amidoidrolases/metabolismo
[Mh] Termos MeSH secundário: Actinomycetales/química
Amidoidrolases/química
Sequência de Aminoácidos
Clonagem Molecular
Cosmídeos
Escherichia coli/genética
Biblioteca Gênica
Concentração de Íons de Hidrogênio
Dados de Sequência Molecular
Mutagênese Sítio-Dirigida
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Recombinant Proteins); EC 3.5.- (Amidohydrolases); EC 3.5.2.- (gamma-lactamase)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150319
[Lr] Data última revisão:
150319
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141025
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-014-6114-8


  6 / 3119 MEDLINE  
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[PMID]:25014195
[Au] Autor:Ullah I; Jang EK; Kim MS; Shin JH; Park GS; Khan AR; Hong SJ; Jung BK; Choi J; Park Y; Kwak Y; Shin JH
[Ad] Endereço:School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea. ihsanknu@gmail.com.
[Ti] Título:Identification and characterization of the insecticidal toxin "makes caterpillars floppy" in Photorhabdus temperata M1021 using a cosmid library.
[So] Source:Toxins (Basel);6(7):2024-40, 2014 Jul 10.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Photorhabdus temperata is an entomopathogenic enterobacterium; it is a nematode symbiont that possesses pathogenicity islands involved in insect virulence. Herein, we constructed a P. temperata M1021 cosmid library in Escherichia coli XL1-Blue MRF` and obtained 7.14 × 105 clones. However, only 1020 physiologically active clones were screened for insect virulence factors by injection of each E. coli cosmid clone into Galleria mellonella and Tenebrio molitor larvae. A single cosmid clone, PtC1015, was consequently selected due to its characteristic virulent properties, e.g., loss of body turgor followed by death of larvae when the clone was injected into the hemocoel. The sequence alignment against the available sequences in Swiss-Prot and NCBI databases, confirmed the presence of the mcf gene homolog in the genome of P. temperata M1021 showing 85% homology and 98% query coverage with the P. luminescens counterpart. Furthermore, a 2932 amino acid long Mcf protein revealed limited similarity with three protein domains. The N-terminus of the Mcf encompassed consensus sequence for a BH3 domain, the central region revealed similarity to toxin B, and the C-terminus of Mcf revealed similarity to the bacterial export domain of ApxIVA, an RTX-like toxin. In short, the Mcf toxin is likely to play a role in the elimination of insect pests, making it a promising model for use in the agricultural field.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Toxinas Bacterianas
Inseticidas
Photorhabdus
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/toxicidade
Toxinas Bacterianas/genética
Toxinas Bacterianas/isolamento & purificação
Toxinas Bacterianas/toxicidade
Cosmídeos
Escherichia coli/genética
Inseticidas/isolamento & purificação
Inseticidas/toxicidade
Larva/efeitos dos fármacos
Dados de Sequência Molecular
Mariposas/efeitos dos fármacos
Filogenia
Tenebrio/efeitos dos fármacos
Fatores de Virulência/genética
Fatores de Virulência/isolamento & purificação
Fatores de Virulência/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Insecticides); 0 (Virulence Factors)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140712
[St] Status:MEDLINE
[do] DOI:10.3390/toxins6072024


  7 / 3119 MEDLINE  
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[PMID]:24911009
[Au] Autor:Lam KN; Hall MW; Engel K; Vey G; Cheng J; Neufeld JD; Charles TC
[Ad] Endereço:Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
[Ti] Título:Evaluation of a pooled strategy for high-throughput sequencing of cosmid clones from metagenomic libraries.
[So] Source:PLoS One;9(6):e98968, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High-throughput sequencing methods have been instrumental in the growing field of metagenomics, with technological improvements enabling greater throughput at decreased costs. Nonetheless, the economy of high-throughput sequencing cannot be fully leveraged in the subdiscipline of functional metagenomics. In this area of research, environmental DNA is typically cloned to generate large-insert libraries from which individual clones are isolated, based on specific activities of interest. Sequence data are required for complete characterization of such clones, but the sequencing of a large set of clones requires individual barcode-based sample preparation; this can become costly, as the cost of clone barcoding scales linearly with the number of clones processed, and thus sequencing a large number of metagenomic clones often remains cost-prohibitive. We investigated a hybrid Sanger/Illumina pooled sequencing strategy that omits barcoding altogether, and we evaluated this strategy by comparing the pooled sequencing results to reference sequence data obtained from traditional barcode-based sequencing of the same set of clones. Using identity and coverage metrics in our evaluation, we show that pooled sequencing can generate high-quality sequence data, without producing problematic chimeras. Though caveats of a pooled strategy exist and further optimization of the method is required to improve recovery of complete clone sequences and to avoid circumstances that generate unrecoverable clone sequences, our results demonstrate that pooled sequencing represents an effective and low-cost alternative for sequencing large sets of metagenomic clones.
[Mh] Termos MeSH primário: Cosmídeos/genética
Biblioteca Gênica
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Metagenômica
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Clonagem Molecular
Seres Humanos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140610
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0098968


  8 / 3119 MEDLINE  
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[PMID]:24633227
[Au] Autor:Braña AF; Rodríguez M; Pahari P; Rohr J; García LA; Blanco G
[Ad] Endereço:Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006, Oviedo, Spain.
[Ti] Título:Activation and silencing of secondary metabolites in Streptomyces albus and Streptomyces lividans after transformation with cosmids containing the thienamycin gene cluster from Streptomyces cattleya.
[So] Source:Arch Microbiol;196(5):345-55, 2014 May.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Activation and silencing of antibiotic production was achieved in Streptomyces albus J1074 and Streptomyces lividans TK21 after introduction of genes within the thienamycin cluster from S. cattleya. Dramatic phenotypic and metabolic changes, involving activation of multiple silent secondary metabolites and silencing of others normally produced, were found in recombinant strains harbouring the thienamycin cluster in comparison to the parental strains. In S. albus, ultra-performance liquid chromatography purification and NMR structural elucidation revealed the identity of four structurally related activated compounds: the antibiotics paulomycins A, B and the paulomenols A and B. Four volatile compounds whose biosynthesis was switched off were identified by gas chromatography-mass spectrometry analyses and databases comparison as pyrazines; including tetramethylpyrazine, a compound with important clinical applications to our knowledge never reported to be produced by Streptomyces. In addition, this work revealed the potential of S. albus to produce many others secondary metabolites normally obtained from plants, including compounds of medical relevance as dihydro-ß-agarofuran and of interest in perfume industry as ß-patchoulene, suggesting that it might be an alternative model for their industrial production. In S. lividans, actinorhodins production was strongly activated in the recombinant strains whereas undecylprodigiosins were significantly reduced. Activation of cryptic metabolites in Streptomyces species might represent an alternative approach for pharmaceutical drug discovery.
[Mh] Termos MeSH primário: Antibacterianos/biossíntese
Família Multigênica
Metabolismo Secundário/genética
Streptomyces lividans/metabolismo
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/química
Cosmídeos
Inativação Gênica
Estrutura Molecular
Streptomyces/genética
Streptomyces lividans/genética
Tienamicinas/biossíntese
Transformação Genética
Compostos Orgânicos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Thienamycins); 0 (Volatile Organic Compounds); WMW5I5964P (thienamycin)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140318
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-014-0977-z


  9 / 3119 MEDLINE  
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[PMID]:24598591
[Au] Autor:Flinspach K; Kapitzke C; Tocchetti A; Sosio M; Apel AK
[Ad] Endereço:Pharmaceutical Institute, University of Tübingen, Tübingen, Germany; German Centre for Infection Research (DZIF), partner site Tübingen, Tübingen, Germany.
[Ti] Título:Heterologous expression of the thiopeptide antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor requires deletion of ribosomal genes from the expression construct.
[So] Source:PLoS One;9(3):e90499, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:GE2270 is a thiopeptide antibiotic generated by extensive posttranslational modifications of a ribosomally generated precursor peptide. Thiopeptides are especially active against Gram-positive bacteria, including methicillin resistant Staphylococcus aureus (MRSA). In this study the GE2270 biosynthetic gene cluster (pbt) from Planobispora rosea ATCC 53733 was successfully expressed in the heterologous host strain Streptomyces coelicolor M1146. Notably, exconjugants containing the pbt gene cluster could only be obtained after deletion of the major part of the ribosomal genes flanking the gene cluster. This is a striking example that genes belonging to primary metabolism can prevent the successful conjugative transfer of DNA from phylogenetic distant species and thus complicate heterologous expression of secondary metabolite gene clusters. GE2270 production in the heterologous producer strain increased after introduction of the constitutive ermE* promoter upstream of the GE2270 resistance gene tuf from P. rosea. Insertion of the inducible tcp830 promoter resulted in inducible GE2270 production. When the regulatory gene pbtR was deleted, the resulting strain ceased to produce GE2270, suggesting an essential role of PbtR as a putative transcriptional activator of GE2270 expression.
[Mh] Termos MeSH primário: Actinomycetales/genética
Antibacterianos/biossíntese
Proteínas de Bactérias/biossíntese
DNA Ribossômico/genética
Peptídeos Cíclicos/biossíntese
Streptomyces coelicolor/genética
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/farmacologia
Clonagem Molecular
Cosmídeos/genética
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Farmacorresistência Bacteriana
Regulação Bacteriana da Expressão Gênica
Genes Bacterianos
Tipagem Molecular
Família Multigênica
Peptídeos Cíclicos/genética
Peptídeos Cíclicos/farmacologia
Filogenia
Regiões Promotoras Genéticas
RNA Ribossômico 16S/genética
Streptomyces coelicolor/efeitos dos fármacos
Tiazóis/farmacologia
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Ribosomal); 0 (Peptides, Cyclic); 0 (RNA, Ribosomal, 16S); 0 (Thiazoles); JB4J58L2K1 (GE 2270 A)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140307
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0090499


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[PMID]:24495694
[Au] Autor:Cheng J; Pinnell L; Engel K; Neufeld JD; Charles TC
[Ad] Endereço:Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
[Ti] Título:Versatile broad-host-range cosmids for construction of high quality metagenomic libraries.
[So] Source:J Microbiol Methods;99:27-34, 2014 Apr.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We constructed IncP broad-host-range Gateway® entry cosmids pJC8 and pJC24, which replicate in diverse Proteobacteria. We demonstrate the functionality of these vectors by extracting, purifying, and size-selecting metagenomic DNA from agricultural corn and wheat soils, followed by cloning into pJC8. Metagenomic DNA libraries of 8×10(4) (corn soil) and 9×10(6) (wheat soil) clones were generated for functional screening. The DNA cloned in these libraries can be transferred from these recombinant cosmids to Gateway® destination vectors for specialized screening purposes. Those library clones are available from the Canadian MetaMicroBiome Library project (http://www.cm2bl.org/).
[Mh] Termos MeSH primário: Cosmídeos
Biblioteca Gênica
Genética Microbiana/métodos
Metagenômica/métodos
Biologia Molecular/métodos
Proteobactérias/genética
[Mh] Termos MeSH secundário: Especificidade de Hospedeiro
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140321
[Lr] Data última revisão:
140321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140206
[St] Status:MEDLINE



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