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[PMID]:28985409
[Au] Autor:Grieb MS; Nivina A; Cheeseman BL; Hartmann A; Mazel D; Schlierf M
[Ad] Endereço:B CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307 Dresden, Germany.
[Ti] Título:Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality.
[So] Source:Nucleic Acids Res;45(18):10555-10563, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biologically functional DNA hairpins are found in archaea, prokaryotes and eukaryotes, playing essential roles in various DNA transactions. However, during DNA replication, hairpin formation can stall the polymerase and is therefore prevented by the single-stranded DNA binding protein (SSB). Here, we address the question how hairpins maintain their functional secondary structure despite SSB's presence. As a model hairpin, we used the recombinogenic form of the attC site, essential for capturing antibiotic-resistance genes in the integrons of bacteria. We found that attC hairpins have a conserved high GC-content near their apical loop that creates a dynamic equilibrium between attC fully opened by SSB and a partially structured attC-6-SSB complex. This complex is recognized by the recombinase IntI, which extrudes the hairpin upon binding while displacing SSB. We anticipate that this intriguing regulation mechanism using a base pair distribution to balance hairpin structure formation and genetic stability is key to the dissemination of antibiotic resistance genes among bacteria and might be conserved among other functional hairpins.
[Mh] Termos MeSH primário: Sítios de Ligação Microbiológicos
DNA Bacteriano/química
DNA de Cadeia Simples
Proteínas de Ligação a DNA/metabolismo
Proteínas de Escherichia coli/metabolismo
Integrons
[Mh] Termos MeSH secundário: DNA Bacteriano/metabolismo
Integrases/metabolismo
Conformação de Ácido Nucleico
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Single-Stranded); 0 (DNA-Binding Proteins); 0 (Escherichia coli Proteins); 0 (SSB protein, E coli); EC 2.7.7.- (Integrases); EC 2.7.7.- (integron integrase IntI1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx670


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[PMID]:28910343
[Au] Autor:Liang X; Liao C; Soupir ML; Jarboe LR; Thompson ML; Dixon PM
[Ad] Endereço:Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, United States of America.
[Ti] Título:Escherichia coli attachment to model particulates: The effects of bacterial cell characteristics and particulate properties.
[So] Source:PLoS One;12(9):e0184664, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:E. coli bacteria move in streams freely in a planktonic state or attached to suspended particulates. Attachment is a dynamic process, and the fraction of attached microorganisms is thought to be affected by both bacterial characteristics and particulate properties. In this study, we investigated how the properties of cell surfaces and stream particulates influence attachment. Attachment assays were conducted for 77 E. coli strains and three model particulates (ferrihydrite, Ca-montmorillonite, or corn stover) under environmentally relevant conditions. Surface area, particle size distribution, and total carbon content were determined for each type of particulate. Among the three particulates, attachment fractions to corn stover were significantly larger than the attachments to 2-line ferrihydrite (p-value = 0.0036) and Ca-montmorillonite (p-value = 0.022). Furthermore, attachment to Ca-montmorillonite and corn stover was successfully modeled by a Generalized Additive Model (GAM) using cell characteristics as predictor variables. The natural logarithm of the net charge on the bacterial surface had a significant, positive, and linear impact on the attachment of E. coli bacteria to Ca-montmorillonite (p-value = 0.013), but it did not significantly impact the attachment to corn stover (p-value = 0.36). The large diversities in cell characteristics among 77 E. coli strains, particulate properties, and attachment fractions clearly demonstrated the inadequacy of using a static parameter or linear coefficient to predict the attachment behavior of E. coli in stream water quality models.
[Mh] Termos MeSH primário: Aderência Bacteriana
Escherichia coli/fisiologia
[Mh] Termos MeSH secundário: Algoritmos
Sítios de Ligação Microbiológicos
Bentonita
Tamanho da Partícula
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1302-78-9 (Bentonite)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184664


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[PMID]:28666339
[Au] Autor:Olorunniji FJ; McPherson AL; Rosser SJ; Smith MCM; Colloms SD; Stark WM
[Ad] Endereço:Institute of Molecular, Cell and Systems Biology, University of Glasgow, Bower Building, Glasgow G12 8QQ, UK.
[Ti] Título:Control of serine integrase recombination directionality by fusion with the directionality factor.
[So] Source:Nucleic Acids Res;45(14):8635-8645, 2017 Aug 21.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL and attR sites. The 'reverse' reaction requires another phage-encoded protein called the recombination directionality factor (RDF) in addition to integrase; RDF activates attL × attR recombination and inhibits attP × attB recombination. We show here that serine integrases can be fused to their cognate RDFs to create single proteins that catalyse efficient attL × attR recombination in vivo and in vitro, whereas attP × attB recombination efficiency is reduced. We provide evidence that activation of attL × attR recombination involves intra-subunit contacts between the integrase and RDF moieties of the fusion protein. Minor changes in the length and sequence of the integrase-RDF linker peptide did not affect fusion protein recombination activity. The efficiency and single-protein convenience of integrase-RDF fusion proteins make them potentially very advantageous for biotechnology/synthetic biology applications. Here, we demonstrate efficient gene cassette replacement in a synthetic metabolic pathway gene array as a proof of principle.
[Mh] Termos MeSH primário: Bacteriófagos/enzimologia
Integrases/metabolismo
Recombinação Genética
Serina/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação Microbiológicos/genética
Bacteriófagos/genética
Fusão Gênica
Integrases/genética
Modelos Genéticos
Oligonucleotídeos/genética
Oligonucleotídeos/metabolismo
Plasmídeos/genética
Plasmídeos/metabolismo
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Serina/genética
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotides); 0 (Recombinant Fusion Proteins); 0 (Viral Proteins); 452VLY9402 (Serine); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170702
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx567


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[PMID]:28549184
[Au] Autor:Gupta K; Sharp R; Yuan JB; Li H; Van Duyne GD
[Ad] Endereço:Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 10104, USA.
[Ti] Título:Coiled-coil interactions mediate serine integrase directionality.
[So] Source:Nucleic Acids Res;45(12):7339-7353, 2017 Jul 07.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Serine integrases are bacteriophage enzymes that carry out site-specific integration and excision of their viral genomes. The integration reaction is highly directional; recombination between the phage attachment site attP and the host attachment site attB to form the hybrid sites attL and attR is essentially irreversible. In a recent model, extended coiled-coil (CC) domains in the integrase subunits are proposed to interact in a way that favors the attPxattB reaction but inhibits the attLxattR reaction. Here, we show for the Listeria innocua integrase (LI Int) system that the CC domain promotes self-interaction in isolated Int and when Int is bound to attachment sites. Three independent crystal structures of the CC domain reveal the molecular nature of the CC dimer interface. Alanine substitutions of key residues in the interface support the functional significance of the structural model and indicate that the same interaction is responsible for promoting integration and for inhibiting excision. An updated model of a LI Int•attL complex that incorporates the high resolution CC dimer structure provides insights that help to explain the unusual CC dimer structure and potential sources of stability in Int•attL and Int•attR complexes. Together, the data provide a molecular basis for understanding serine integrase directionality.
[Mh] Termos MeSH primário: Sítios de Ligação Microbiológicos
Bacteriófagos/genética
DNA Bacteriano/química
Integrases/química
Listeria/virologia
Serina/química
Proteínas Virais/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacteriófagos/metabolismo
Sítios de Ligação
Clonagem Molecular
Cristalografia por Raios X
DNA Bacteriano/genética
DNA Bacteriano/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Integrases/genética
Integrases/metabolismo
Cinética
Listeria/genética
Listeria/metabolismo
Modelos Moleculares
Mutagênese Insercional
Ligação Proteica
Conformação Proteica em alfa-Hélice
Domínios e Motivos de Interação entre Proteínas
Multimerização Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Recombinação Genética
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Serina/metabolismo
Especificidade por Substrato
Termodinâmica
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Recombinant Proteins); 0 (Viral Proteins); 452VLY9402 (Serine); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx474


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[PMID]:28400295
[Au] Autor:Ahmadi M; Mahboudi F; Ahmadi S; Ebadat S; Nematpour F; Akbari Eidgahi MR; Davami F
[Ad] Endereço:Medical Biotechnology Department, Semnan University of Medical Sciences, Semnan, Iran; Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
[Ti] Título:PhiC31 integrase can improve the efficiency of different construct designs for monoclonal antibody expression in CHO cells.
[So] Source:Protein Expr Purif;134:89-95, 2017 Jun.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Several types of expression vectors have been used for recombinant protein expression in Chinese hamster ovary cells (CHO) which usually result in variable and unstable levels of expression. METHODS AND RESULTS: In this study, we have compared the mAb0014 expression level of single ORF/IRES vector and dual ORF vector in the presence and absence of phiC31 integrase targeting system. Both expression vectors contain an elongation factor 1α (EF1α) promoter upstream of LC and harboring an attB site. CHO-S cells were co-transfected with single ORF/IRES or dual ORF vectors along with a phiC31 integrase expression vector which can catalyze recombination between attB site and pseudo-attP sites presented in the mammalian genome. Our results demonstrated that dual ORF vector in the presence of phiC31 integrase expression vectors (+FC31 2P) generated more recombinant antibody in comparison to its negative control (-FC31 2P). Moreover, both of +FC31 2P and -FC31 2P cell pools yield higher recombinant protein in comparison to single ORF/IRES vector (FC31 IRES) cell pools. Stability of expression in phiC31 co-transfected cell pools (+FC31 2P and +FC31 IRES) had no considerable changes. CONCLUSIONS: Our results indicated that the dual ORF vector using integrase can support the generation of cell lines with stable transgene expression at an elevated mAb relative to single ORF/IRES vector.
[Mh] Termos MeSH primário: Anticorpos Monoclonais
Expressão Gênica
Vetores Genéticos/genética
Integrases
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/biossíntese
Anticorpos Monoclonais/genética
Sítios de Ligação Microbiológicos
Células CHO
Cricetinae
Cricetulus
Integrases/biossíntese
Integrases/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Recombinant Proteins); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE


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[PMID]:28355179
[Au] Autor:Modell JW; Jiang W; Marraffini LA
[Ad] Endereço:Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
[Ti] Título:CRISPR-Cas systems exploit viral DNA injection to establish and maintain adaptive immunity.
[So] Source:Nature;544(7648):101-104, 2017 04 06.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems provide protection against viral and plasmid infection by capturing short DNA sequences from these invaders and integrating them into the CRISPR locus of the prokaryotic host. These sequences, known as spacers, are transcribed into short CRISPR RNA guides that specify the cleavage site of Cas nucleases in the genome of the invader. It is not known when spacer sequences are acquired during viral infection. Here, to investigate this, we tracked spacer acquisition in Staphylococcus aureus cells harbouring a type II CRISPR-Cas9 system after infection with the staphylococcal bacteriophage Ï•12. We found that new spacers were acquired immediately after infection preferentially from the cos site, the viral free DNA end that is first injected into the cell. Analysis of spacer acquisition after infection with mutant phages demonstrated that most spacers are acquired during DNA injection, but not during other stages of the viral cycle that produce free DNA ends, such as DNA replication or packaging. Finally, we showed that spacers acquired from early-injected genomic regions, which direct Cas9 cleavage of the viral DNA immediately after infection, provide better immunity than spacers acquired from late-injected regions. Our results reveal that CRISPR-Cas systems exploit the phage life cycle to generate a pattern of spacer acquisition that ensures a successful CRISPR immune response.
[Mh] Termos MeSH primário: Fagos Bacilares/genética
Fagos Bacilares/imunologia
Sistemas CRISPR-Cas/genética
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética
DNA Viral/genética
Staphylococcus aureus/imunologia
Staphylococcus aureus/virologia
[Mh] Termos MeSH secundário: Sítios de Ligação Microbiológicos/genética
Fagos Bacilares/crescimento & desenvolvimento
Fagos Bacilares/fisiologia
Proteínas Associadas a CRISPR/metabolismo
Sistemas CRISPR-Cas/imunologia
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/imunologia
DNA Viral/imunologia
DNA Viral/metabolismo
Mutação
Staphylococcus aureus/genética
Fatores de Tempo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CRISPR-Associated Proteins); 0 (DNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1038/nature21719


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[PMID]:28252198
[Au] Autor:McLellan MA; Rosenthal NA; Pinto AR
[Ad] Endereço:The Jackson Laboratory, Bar Harbor, Maine.
[Ti] Título:Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations.
[So] Source:Curr Protoc Mouse Biol;7(1):1-12, 2017 Mar 02.
[Is] ISSN:2161-2617
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. While the use of the cre-loxP system is becoming increasingly widespread, in particular because of growing availability of conditional mouse mutants, many considerations need to be taken into account when utilizing the cre-loxP system. This review provides an overview of the cre-loxP system and its various permutations. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration. © 2017 by John Wiley & Sons, Inc.
[Mh] Termos MeSH primário: Sítios de Ligação Microbiológicos/genética
Engenharia Genética/métodos
Integrases/genética
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Bacteriófago P1/genética
Sequência de Bases
Sítios de Ligação/genética
Doxiciclina/farmacologia
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Ligantes
Camundongos
Modelos Genéticos
Receptores Estrogênicos/genética
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Ligands); 0 (Receptors, Estrogen); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases); N12000U13O (Doxycycline)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1002/cpmo.22


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[PMID]:28077763
[Au] Autor:Pokhilko A; Zhao J; Stark WM; Colloms SD; Ebenhöh O
[Ad] Endereço:Institute of Molecular, Cell and Systems Biology, University of Glasgow, Glasgow G12 8QQ, UK.
[Ti] Título:A simplified mathematical model of directional DNA site-specific recombination by serine integrases.
[So] Source:J R Soc Interface;14(126), 2017 Jan.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Serine integrases catalyse site-specific recombination to integrate and excise bacteriophage genomes into and out of their host's genome. These enzymes exhibit remarkable directionality; in the presence of the integrase alone, recombination between attP and attB DNA sites is efficient and irreversible, giving attL and attR products which do not recombine further. However, in the presence of the bacteriophage-encoded recombination directionality factor (RDF), integrase efficiently promotes recombination between attL and attR to re-form attP and attB The DNA substrates and products of both reactions are approximately isoenergetic, and no cofactors (such as adenosine triphosphate) are required for recombination. The thermodynamic driving force for directionality of these reactions is thus enigmatic. Here, we present a minimal mathematical model which can explain the directionality and regulation of both 'forward' and 'reverse' reactions. In this model, the substrates of the 'forbidden' reactions (between attL and attR in the absence of RDF, attP and attB in the presence of RDF) are trapped as inactive protein-DNA complexes, ensuring that these 'forbidden' reactions are extremely slow. The model is in good agreement with the observed in vitro kinetics of recombination by Ï•C31 integrase, and defines core features of the system necessary and sufficient for directionality.
[Mh] Termos MeSH primário: Sítios de Ligação Microbiológicos
DNA/química
Integrases/química
Modelos Químicos
Modelos Genéticos
Recombinação Genética
[Mh] Termos MeSH secundário: DNA/metabolismo
Integrases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE


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[PMID]:28011085
[Au] Autor:Zhang S; Ban A; Ebara N; Mizutani O; Tanaka M; Shintani T; Gomi K
[Ad] Endereço:Laboratory of Bioindustrial Genomics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
[Ti] Título:Self-excising Cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in Aspergillus oryzae.
[So] Source:J Biosci Bioeng;123(4):403-411, 2017 Apr.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:In this study, we developed a self-excising Cre/loxP-mediated marker recycling system with mutated lox sequences to introduce a number of biosynthetic genes into Aspergillus oryzae. To construct the self-excising marker cassette, both the selectable marker, the Aspergillus nidulans adeA gene, and the Cre recombinase gene (cre), conditionally expressed by the xylanase-encoding gene promoter, were designed to be located between the mutant lox sequences, lox66 and lox71. However, construction of the plasmid failed, possibly owing to a slight expression of cre downstream of the fungal gene promoter in Escherichia coli. Hence, to avoid the excision of the cassette in E. coli, a 71-bp intron of the A. oryzae xynG2 gene was inserted into the cre gene. The A. oryzae adeA deletion mutant was transformed with the resulting plasmid in the presence of glucose, and the transformants were cultured in medium containing xylose as the sole carbon source. PCR analysis of genomic DNA from resultant colonies revealed the excision of both the marker and Cre expression construct, indicating that the self-excising marker cassette was efficient at removing the selectable marker. Using the marker recycling system, hyperproduction of kojic acid could be achieved in A. oryzae by the introduction of two genes that encode oxidoreductase and transporter. Furthermore, we also constructed an alternative marker recycling cassette bearing the A. nidulans pyrithiamine resistant gene (ptrA) as a dominant selectable marker.
[Mh] Termos MeSH primário: Aspergillus oryzae/genética
Sítios de Ligação Microbiológicos/genética
Deleção de Genes
Integrases/metabolismo
Mutagênese Insercional/métodos
Mutagênese Sítio-Dirigida/métodos
[Mh] Termos MeSH secundário: Aspergillus nidulans/genética
Escherichia coli/genética
Genes Fúngicos/genética
Marcadores Genéticos/genética
Integrases/genética
Plasmídeos/genética
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE


  10 / 702 MEDLINE  
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[PMID]:27959343
[Au] Autor:Martínez-Rubio R; Quiles-Puchalt N; Martí M; Humphrey S; Ram G; Smyth D; Chen J; Novick RP; Penadés JR
[Ad] Endereço:Departamento de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Spain.
[Ti] Título:Phage-inducible islands in the Gram-positive cocci.
[So] Source:ISME J;11(4):1029-1042, 2017 Apr.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The SaPIs are a cohesive subfamily of extremely common phage-inducible chromosomal islands (PICIs) that reside quiescently at specific att sites in the staphylococcal chromosome and are induced by helper phages to excise and replicate. They are usually packaged in small capsids composed of phage virion proteins, giving rise to very high transfer frequencies, which they enhance by interfering with helper phage reproduction. As the SaPIs represent a highly successful biological strategy, with many natural Staphylococcus aureus strains containing two or more, we assumed that similar elements would be widespread in the Gram-positive cocci. On the basis of resemblance to the paradigmatic SaPI genome, we have readily identified large cohesive families of similar elements in the lactococci and pneumococci/streptococci plus a few such elements in Enterococcus faecalis. Based on extensive ortholog analyses, we found that the PICI elements in the four different genera all represent distinct but parallel lineages, suggesting that they represent convergent evolution towards a highly successful lifestyle. We have characterized in depth the enterococcal element, EfCIV583, and have shown that it very closely resembles the SaPIs in functionality as well as in genome organization, setting the stage for expansion of the study of elements of this type. In summary, our findings greatly broaden the PICI family to include elements from at least three genera of cocci.
[Mh] Termos MeSH primário: Bacteriófagos/fisiologia
Regulação Bacteriana da Expressão Gênica/fisiologia
Ilhas Genômicas
Cocos Gram-Positivos/virologia
[Mh] Termos MeSH secundário: Sítios de Ligação Microbiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.163



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