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[PMID]:29202688
[Au] Autor:Abrahamian M; Kagda M; Ah-Fong AMV; Judelson HS
[Ad] Endereço:Department of Microbiology and Plant Pathology, University of California, Riverside, CA, 92521, USA.
[Ti] Título:Rethinking the evolution of eukaryotic metabolism: novel cellular partitioning of enzymes in stramenopiles links serine biosynthesis to glycolysis in mitochondria.
[So] Source:BMC Evol Biol;17(1):241, 2017 Dec 04.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: An important feature of eukaryotic evolution is metabolic compartmentalization, in which certain pathways are restricted to the cytosol or specific organelles. Glycolysis in eukaryotes is described as a cytosolic process. The universality of this canon has been challenged by recent genome data that suggest that some glycolytic enzymes made by stramenopiles bear mitochondrial targeting peptides. RESULTS: Mining of oomycete, diatom, and brown algal genomes indicates that stramenopiles encode two forms of enzymes for the second half of glycolysis, one with and the other without mitochondrial targeting peptides. The predicted mitochondrial targeting was confirmed by using fluorescent tags to localize phosphoglycerate kinase, phosphoglycerate mutase, and pyruvate kinase in Phytophthora infestans, the oomycete that causes potato blight. A genome-wide search for other enzymes with atypical mitochondrial locations identified phosphoglycerate dehydrogenase, phosphoserine aminotransferase, and phosphoserine phosphatase, which form a pathway for generating serine from the glycolytic intermediate 3-phosphoglycerate. Fluorescent tags confirmed the delivery of these serine biosynthetic enzymes to P. infestans mitochondria. A cytosolic form of this serine biosynthetic pathway, which occurs in most eukaryotes, is missing from oomycetes and most other stramenopiles. The glycolysis and serine metabolism pathways of oomycetes appear to be mosaics of enzymes with different ancestries. While some of the noncanonical oomycete mitochondrial enzymes have the closest affinity in phylogenetic analyses with proteins from other stramenopiles, others cluster with bacterial, plant, or animal proteins. The genes encoding the mitochondrial phosphoglycerate kinase and serine-forming enzymes are physically linked on oomycete chromosomes, which suggests a shared origin. CONCLUSIONS: Stramenopile metabolism appears to have been shaped through the acquisition of genes by descent and lateral or endosymbiotic gene transfer, along with the targeting of the proteins to locations that are novel compared to other eukaryotes. Colocalization of the glycolytic and serine biosynthesis enzymes in mitochondria is apparently necessary since they share a common intermediate. The results indicate that descriptions of metabolism in textbooks do not cover the full diversity of eukaryotic biology.
[Mh] Termos MeSH primário: Evolução Biológica
Células Eucarióticas/metabolismo
Glicólise
Mitocôndrias/metabolismo
Serina/biossíntese
Estramenópilas/enzimologia
Estramenópilas/metabolismo
[Mh] Termos MeSH secundário: Animais
Citosol
Genes
Mitocôndrias/genética
Oomicetos/metabolismo
Fosforilação
Filogenia
Phytophthora infestans/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
452VLY9402 (Serine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-1087-8


  2 / 54260 MEDLINE  
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[PMID]:29193199
[Au] Autor:Liu C; Xie Y; Sun B; Geng F; Zhang F; Guo Q; Wu H; Yu B; Wu J; Yu X; Kong W; Zhang H
[Ad] Endereço:National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun, China.
[Ti] Título:MUC1- and Survivin-based DNA Vaccine Combining Immunoadjuvants CpG and interleukin-2 in a Bicistronic Expression Plasmid Generates Specific Immune Responses and Antitumour Effects in a Murine Colorectal Carcinoma Model.
[So] Source:Scand J Immunol;87(2):63-72, 2018 Feb.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DNA vaccination is a promising cancer treatment due to its safety, but poor immunogenicity limits its application. However, immunoadjuvants, heterogeneous prime-boost strategies and combination with conventional treatments can be used to improve the antitumour immune effects. A CpG motif and interleukin-2 (IL-2) cytokine are often used as adjuvants. In this study, a DNA vaccine containing a CpG motif was constructed to evaluate its adjuvant effect. The results show that the cytotoxicity of the DNA vaccine was increased fivefold, and survival lifetime was prolonged twofold by the CpG motif adjuvant. To simplify the industrial production process, a bicistronic plasmid was constructed to carry the fusion genes of survivin/MUC1 (MS) and IL-2 and with a CpG motif in its backbone. The results showed that the antitumour effect of the bicistronic vaccine was the same as that of the two vaccine co-injected regime. Furthermore, the vaccine could suppress metastatic tumour foci by 69.1% in colorectal carcinoma-bearing mice. Moreover, the vaccine induced survivin- and MUC1-specific immune responses in splenocytes and induced the immune promoting factor CCL-19 and GM-CSF upregulated, while metastatic-associated factor MMP-9 and immunosuppressing factor PD-L1 downregulated in tumour tissue. When combining the vaccine with the chemotherapy drug oxaliplatin, the survival was prolonged by about 2.5-fold. In conclusion, the DNA vaccine containing a CpG motif in bicistronic form showed good effects on colorectal cancer by inhibiting both tumour growth and metastasis, and combination with oxaliplatin could improve its antitumour effects.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/genética
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Vacinas Anticâncer/imunologia
Neoplasias Colorretais/imunologia
Proteínas Inibidoras de Apoptose/genética
Mucina-1/genética
Oligodesoxirribonucleotídeos/genética
Compostos Organoplatínicos/uso terapêutico
Proteínas Repressoras/genética
Vacinas de DNA/imunologia
[Mh] Termos MeSH secundário: Animais
Vacinas Anticâncer/genética
Processos de Crescimento Celular
Linhagem Celular Tumoral
Neoplasias Colorretais/terapia
Modelos Animais de Doenças
Feminino
Genes/genética
Seres Humanos
Imunidade
Interleucina-2/administração & dosagem
Interleucina-2/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Metástase Neoplásica
Plasmídeos
Vacinas de DNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Birc5 protein, mouse); 0 (CPG-oligonucleotide); 0 (Cancer Vaccines); 0 (Inhibitor of Apoptosis Proteins); 0 (Interleukin-2); 0 (Mucin-1); 0 (Oligodeoxyribonucleotides); 0 (Organoplatinum Compounds); 0 (Repressor Proteins); 0 (Vaccines, DNA); 0 (muc1 protein, mouse); 04ZR38536J (oxaliplatin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12633


  3 / 54260 MEDLINE  
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[PMID]:29088275
[Au] Autor:Sikdar S; Datta S; Datta S
[Ad] Endereço:Department of Biostatistics, University of Florida, Gainesville, Florida, United States of America.
[Ti] Título:EAMA: Empirically adjusted meta-analysis for large-scale simultaneous hypothesis testing in genomic experiments.
[So] Source:PLoS One;12(10):e0187287, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent developments in high throughput genomic assays have opened up the possibility of testing hundreds and thousands of genes simultaneously. However, adhering to the regular statistical assumptions regarding the null distributions of test statistics in such large-scale multiple testing frameworks has the potential of leading to incorrect significance testing results and biased inference. This problem gets worse when one combines results from different independent genomic experiments with a possibility of ending up with gross false discoveries of significant genes. In this article, we develop a meta-analysis method of combining p-values from different independent experiments involving large-scale multiple testing frameworks, through empirical adjustments of the individual test statistics and p-values. Even though, it is based on various existing ideas, this specific combination is novel and potentially useful. Through simulation studies and real genomic datasets we show that our method outperforms the standard meta-analysis approach of significance testing in terms of accurately identifying the truly significant set of genes.
[Mh] Termos MeSH primário: Genômica
Metanálise como Assunto
Estatística como Assunto
[Mh] Termos MeSH secundário: Interpretação Estatística de Dados
Pesquisa Empírica
Expressão Gênica
Genes/genética
Genômica/métodos
Seres Humanos
Neoplasias Pulmonares/genética
Modelos Estatísticos
Análise de Sequência com Séries de Oligonucleotídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171101
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187287


  4 / 54260 MEDLINE  
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[PMID]:28953918
[Au] Autor:Welsh EA; Stewart PA; Kuenzi BM; Eschrich JA
[Ad] Endereço:Cancer Informatics, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida, United States of America.
[Ti] Título:Escape Excel: A tool for preventing gene symbol and accession conversion errors.
[So] Source:PLoS One;12(9):e0185207, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Microsoft Excel automatically converts certain gene symbols, database accessions, and other alphanumeric text into dates, scientific notation, and other numerical representations. These conversions lead to subsequent, irreversible, corruption of the imported text. A recent survey of popular genomic literature estimates that one-fifth of all papers with supplementary gene lists suffer from this issue. RESULTS: Here, we present an open-source tool, Escape Excel, which prevents these erroneous conversions by generating an escaped text file that can be safely imported into Excel. Escape Excel is implemented in a variety of formats (http://www.github.com/pstew/escape_excel), including a command line based Perl script, a Windows-only Excel Add-In, an OS X drag-and-drop application, a simple web-server, and as a Galaxy web environment interface. Test server implementations are accessible as a Galaxy interface (http://apostl.moffitt.org) and simple non-Galaxy web server (http://apostl.moffitt.org:8000/). CONCLUSIONS: Escape Excel detects and escapes a wide variety of problematic text strings so that they are not erroneously converted into other representations upon importation into Excel. Examples of problematic strings include date-like strings, time-like strings, leading zeroes in front of numbers, and long numeric and alphanumeric identifiers that should not be automatically converted into scientific notation. It is hoped that greater awareness of these potential data corruption issues, together with diligent escaping of text files prior to importation into Excel, will help to reduce the amount of Excel-corrupted data in scientific analyses and publications.
[Mh] Termos MeSH primário: Genes
Genômica/métodos
Software
[Mh] Termos MeSH secundário: Internet
Interface Usuário-Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185207


  5 / 54260 MEDLINE  
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[PMID]:28877211
[Au] Autor:Tao X; Liang Y; Yang X; Pang J; Zhong Z; Chen X; Yang Y; Zeng K; Kang R; Lei Y; Ying S; Gong J; Gu Y; Lv X
[Ad] Endereço:Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, Sichuan, China.
[Ti] Título:Transcriptomic profiling in muscle and adipose tissue identifies genes related to growth and lipid deposition.
[So] Source:PLoS One;12(9):e0184120, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Growth performance and meat quality are important traits for the pig industry and consumers. Adipose tissue is the main site at which fat storage and fatty acid synthesis occur. Therefore, we combined high-throughput transcriptomic sequencing in adipose and muscle tissues with the quantification of corresponding phenotypic features using seven Chinese indigenous pig breeds and one Western commercial breed (Yorkshire). We obtained data on 101 phenotypic traits, from which principal component analysis distinguished two groups: one associated with the Chinese breeds and one with Yorkshire. The numbers of differentially expressed genes between all Chinese breeds and Yorkshire were shown to be 673 and 1056 in adipose and muscle tissues, respectively. Functional enrichment analysis revealed that these genes are associated with biological functions and canonical pathways related to oxidoreductase activity, immune response, and metabolic process. Weighted gene coexpression network analysis found more coexpression modules significantly correlated with the measured phenotypic traits in adipose than in muscle, indicating that adipose regulates meat and carcass quality. Using the combination of differential expression, QTL information, gene significance, and module hub genes, we identified a large number of candidate genes potentially related to economically important traits in pig, which should help us improve meat production and quality.
[Mh] Termos MeSH primário: Tecido Adiposo/metabolismo
Metabolismo dos Lipídeos/fisiologia
Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Tecido Adiposo/crescimento & desenvolvimento
Animais
Perfilação da Expressão Gênica
Genes/genética
Genes/fisiologia
Metabolismo dos Lipídeos/genética
Músculo Esquelético/crescimento & desenvolvimento
Fenótipo
Análise de Componente Principal
Característica Quantitativa Herdável
Suínos/genética
Suínos/metabolismo
Transcriptoma/genética
Transcriptoma/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184120


  6 / 54260 MEDLINE  
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[PMID]:28806759
[Au] Autor:Martina F; Beccuti M; Balbo G; Cordero F
[Ad] Endereço:Computer Science Department, University of Turin, Turin, Italy.
[Ti] Título:Peculiar Genes Selection: A new features selection method to improve classification performances in imbalanced data sets.
[So] Source:PLoS One;12(8):e0177475, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High-Throughput technologies provide genomic and trascriptomic data that are suitable for biomarker detection for classification purposes. However, the high dimension of the output of such technologies and the characteristics of the data sets analysed represent an issue for the classification task. Here we present a new feature selection method based on three steps to detect class-specific biomarkers in case of high-dimensional data sets. The first step detects the differentially expressed genes according to the experimental conditions tested in the experimental design, the second step filters out the features with low discriminative power and the third step detects the class-specific features and defines the final biomarker as the union of the class-specific features. The proposed procedure is tested on two microarray datasets, one characterized by a strong imbalance between the size of classes and the other one where the size of classes is perfectly balanced. We show that, using the proposed feature selection procedure, the classification performances of a Support Vector Machine on the imbalanced data set reach a 82% whereas other methods do not exceed 73%. Furthermore, in case of perfectly balanced dataset, the classification performances are comparable with other methods. Finally, the Gene Ontology enrichments performed on the signatures selected with the proposed pipeline, confirm the biological relevance of our methodology. The download of the package with the implementation of Peculiar Genes Selection, 'PGS', is available for R users at: http://github.com/mbeccuti/PGS.
[Mh] Termos MeSH primário: Algoritmos
Biologia Computacional/métodos
Bases de Dados como Assunto
Genes
[Mh] Termos MeSH secundário: Idoso
Feminino
Perfilação da Expressão Gênica
Ontologia Genética
Seres Humanos
Meia-Idade
Neoplasias/genética
Reprodutibilidade dos Testes
Fatores de Transcrição/metabolismo
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177475


  7 / 54260 MEDLINE  
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[PMID]:28800608
[Au] Autor:Mai U; Sayyari E; Mirarab S
[Ad] Endereço:Dept of Computer Science and Engineering, University of California at San Diego, San Diego, CA, United States of America.
[Ti] Título:Minimum variance rooting of phylogenetic trees and implications for species tree reconstruction.
[So] Source:PLoS One;12(8):e0182238, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phylogenetic trees inferred using commonly-used models of sequence evolution are unrooted, but the root position matters both for interpretation and downstream applications. This issue has been long recognized; however, whether the potential for discordance between the species tree and gene trees impacts methods of rooting a phylogenetic tree has not been extensively studied. In this paper, we introduce a new method of rooting a tree based on its branch length distribution; our method, which minimizes the variance of root to tip distances, is inspired by the traditional midpoint rerooting and is justified when deviations from the strict molecular clock are random. Like midpoint rerooting, the method can be implemented in a linear time algorithm. In extensive simulations that consider discordance between gene trees and the species tree, we show that the new method is more accurate than midpoint rerooting, but its relative accuracy compared to using outgroups to root gene trees depends on the size of the dataset and levels of deviations from the strict clock. We show high levels of error for all methods of rooting estimated gene trees due to factors that include effects of gene tree discordance, deviations from the clock, and gene tree estimation error. Our simulations, however, did not reveal significant differences between two equivalent methods for species tree estimation that use rooted and unrooted input, namely, STAR and NJst. Nevertheless, our results point to limitations of existing scalable rooting methods.
[Mh] Termos MeSH primário: Algoritmos
Filogenia
[Mh] Termos MeSH secundário: Simulação por Computador
Bases de Dados como Assunto
Genes
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182238


  8 / 54260 MEDLINE  
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[PMID]:28673819
[Au] Autor:Smith AF; Posakony JW; Rebeiz M
[Ad] Endereço:Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
[Ti] Título:Automated tools for comparative sequence analysis of genic regions using the GenePalette application.
[So] Source:Dev Biol;429(1):158-164, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Comparative sequence analysis methods, such as phylogenetic footprinting, represent one of the most effective ways to decode regulatory sequence functions based upon DNA sequence information alone. The laborious task of assembling orthologous sequences to perform these comparisons is a hurdle to these analyses, which is further aggravated by the relative paucity of tools for visualization of sequence comparisons in large genic regions. Here, we describe a second-generation implementation of the GenePalette DNA sequence analysis software to facilitate comparative studies of gene function and regulation. We have developed an automated module called OrthologGrabber (OG) that performs BLAT searches against the UC Santa Cruz genome database to identify and retrieve segments homologous to a region of interest. Upon acquisition, sequences are compared to identify high-confidence anchor-points, which are graphically displayed. The visualization of anchor-points alongside other DNA features, such as transcription factor binding sites, allows users to precisely examine whether a binding site of interest is conserved, even if the surrounding region exhibits poor sequence identity. This approach also aids in identifying orthologous segments of regulatory DNA, facilitating studies of regulatory sequence evolution. As with previous versions of the software, GenePalette 2.1 takes the form of a platform-independent, single-windowed interface that is simple to use.
[Mh] Termos MeSH primário: Biologia Computacional/métodos
Genes
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Automação
Sequência de Bases
Bases de Dados Genéticas
Genoma
Filogenia
Alinhamento de Sequência
Homologia de Sequência
Interface Usuário-Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE


  9 / 54260 MEDLINE  
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[PMID]:28658281
[Au] Autor:Kringel D; Sisignano M; Zinn S; Lötsch J
[Ad] Endereço:Institute of Clinical Pharmacology, Goethe - University, Frankfurt am Main, Germany.
[Ti] Título:Next-generation sequencing of the human TRPV1 gene and the regulating co-players LTB4R and LTB4R2 based on a custom AmpliSeq™ panel.
[So] Source:PLoS One;12(6):e0180116, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Transient receptor potential cation channel subfamily V member 1 (TRPV1) are sensitive to heat, capsaicin, pungent chemicals and other noxious stimuli. They play important roles in the pain pathway where in concert with proinflammatory factors such as leukotrienes they mediate sensitization and hyperalgesia. TRPV1 is the target of several novel analgesics drugs under development and therefore, TRPV1 genetic variants might represent promising candidates for pharmacogenetic modulators of drug effects. METHODS: A next-generation sequencing (NGS) panel was created for the human TRPV1 gene and in addition, for the leukotriene receptors BLT1 and BLT2 recently described to modulate TRPV1 mediated sensitisation processes rendering the coding genes LTB4R and LTB4R2 important co-players in pharmacogenetic approaches involving TRPV1. The NGS workflow was based on a custom AmpliSeq™ panel and designed for sequencing of human genes on an Ion PGM™ Sequencer. A cohort of 80 healthy subjects of Western European descent was screened to evaluate and validate the detection of exomic sequences of the coding genes with 25 base pair exon padding. RESULTS: The amplicons covered approximately 97% of the target sequence. A median of 2.81 x 106 reads per run was obtained. This identified approximately 140 chromosome loci where nucleotides deviated from the reference sequence GRCh37 hg19 comprising the three genes TRPV1, LTB4R and LTB4R2. Correspondence between NGS and Sanger derived nucleotide sequences was 100%. CONCLUSIONS: Results suggested that the NGS approach based on AmpliSeq™ libraries and Ion Personal Genome Machine (PGM) sequencing is a highly efficient mutation detection method. It is suitable for large-scale sequencing of TRPV1 and functionally related genes. The method adds a large amount of genetic information as a basis for complete analysis of TRPV1 ion channel genetics and its functional consequences.
[Mh] Termos MeSH primário: Receptores do Leucotrieno B4/genética
Canais de Cátion TRPV/genética
[Mh] Termos MeSH secundário: Biblioteca Gênica
Genes/genética
Variação Genética/genética
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (LTB4R protein, human); 0 (LTB4R2 protein, human); 0 (Receptors, Leukotriene B4); 0 (TRPV Cation Channels); 0 (TRPV1 protein, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180116


  10 / 54260 MEDLINE  
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[PMID]:28620030
[Au] Autor:Ziembik MA; Bender TP; Larner JM; Brautigan DL
[Ad] Endereço:Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, VA 22908, U.S.A.
[Ti] Título:Functions of protein phosphatase-6 in NF-κB signaling and in lymphocytes.
[So] Source:Biochem Soc Trans;45(3):693-701, 2017 Jun 15.
[Is] ISSN:1470-8752
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Protein phosphatase-6 (PP6) is a member of the PPP family of Ser/Thr phosphatases involved in intracellular signaling. PP6 is conserved among all eukaryotes, and genetics in model organisms indicates it has non-redundant functions relative to other PPP phosphatases. PP6 functions in association with conserved SAPS subunits and, in vertebrate species, forms heterotrimers with Ankrd subunits. Multiple studies have demonstrated how PP6 exerts negative control at different steps of nuclear factor kappaB signaling. Expression of PP6 catalytic subunit and the PPP6R1 subunit is especially high in hematopoietic cells and lymphoid tissues. Recent efforts at conditionally knocking out genes for PP6c or PP6R1 (SAPS1) have revealed distinctive effects on development of and signaling in lymphocytes.
[Mh] Termos MeSH primário: Linfócitos/metabolismo
NF-kappa B
Fosfoproteínas Fosfatases/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica
Genes
Seres Humanos
Fosfoproteínas Fosfatases/genética
Fosfoproteínas Fosfatases/fisiologia
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (NF-kappa B); EC 3.1.3.16 (Phosphoprotein Phosphatases); EC 3.1.3.16 (protein phosphatase 6)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1042/BST20160169



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