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[PMID]:28454189
[Au] Autor:Pawar RS; Handy SM; Cheng R; Shyong N; Grundel E
[Ad] Endereço:Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U. S. Food and Drug Administration, College Park, MD, USA.
[Ti] Título:Assessment of the Authenticity of Herbal Dietary Supplements: Comparison of Chemical and DNA Barcoding Methods.
[So] Source:Planta Med;83(11):921-936, 2017 Jul.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:About 7 % of the U. S. population reports using botanical dietary supplements. Increased use of such supplements has led to discussions related to their authenticity and quality. Reports of adulteration with substandard materials or pharmaceuticals are of concern because such substitutions, whether inadvertent or deliberate, may reduce the efficacy of specific botanicals or lead to adverse events. Methods for verifying the identity of botanicals include macroscopic and microscopic examinations, chemical analysis, and DNA-based methods including DNA barcoding. Macroscopic and microscopic examinations may fail when a supplement consists of botanicals that have been processed beyond the ability to provide morphological characterizations. Chemical analysis of specific marker compounds encounters problems when these compounds are not distinct to a given species or when purified reference standards are not available. Recent investigations describing DNA barcoding analysis of botanical dietary supplements have raised concerns about the authenticity of the supplements themselves as well as the appropriateness of using DNA barcoding techniques with finished botanical products. We collected 112 market samples of frequently consumed botanical dietary supplements of ginkgo, soy, valerian, yohimbe, and St. John's wort and analyzed each for specific chemical markers (i.e., flavonol glycosides, total isoflavones, total valerenic acids, yohimbine, and hypericins, respectively). We used traditional DNA barcoding techniques targeting the nuclear ITS2 gene and the chloroplast gene A- H on the same samples to determine the presence of DNA of the labelled ingredient. We compared the results obtained by both methods to assess the contribution of each in determining the identity of the samples.
[Mh] Termos MeSH primário: Biomarcadores/análise
Código de Barras de DNA Taxonômico
Suplementos Nutricionais/análise
Plantas Medicinais/química
[Mh] Termos MeSH secundário: DNA de Plantas
Suplementos Nutricionais/normas
Contaminação de Medicamentos
Rotulagem de Medicamentos
Genes de Cloroplastos
Genes de Plantas
Plantas Medicinais/classificação
Controle de Qualidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (DNA, Plant)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171205
[Lr] Data última revisão:
171205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1055/s-0043-107881


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[PMID]:29030254
[Au] Autor:Chen N; Sha LN; Dong ZZ; Tang C; Wang Y; Kang HY; Zhang HQ; Yan XB; Zhou YH; Fan X
[Ad] Endereço:Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, China.
[Ti] Título:Complete structure and variation of the chloroplast genome of Agropyron cristatum (L.) Gaertn.
[So] Source:Gene;640:86-96, 2018 Jan 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Agropyron cristatum (L.) Gaertner, a perennial grass in the tribe Triticeae (Poaceae), is a wild relative of cereal crops that is suitable for genetic improvement. In this study, we first sequenced the complete chloroplast (cp) genome of Ag. cristatum using Hiseq4000 PE150. The Ag. cristatum chloroplast genome is 135,554bp in length, has a typical quadripartite structure and contains 76 protein-coding genes, 29 tRNA genes and four rRNA genes. The cp genome of Ag. cristatum was used for comparison with other seven Triticeae species. One large variable region (800bp), which primarily contained the rpl23 (non-reciprocally translocated from IRs) and accD genes, was detected between rbcL gene and psaI gene within LSC region. The deletion of the accD and translocated rpl23 genes in Ag. cristatum indicated an independent gene-loss events or an additional divergence in Triticeae. Analyses of the dn/ds ratio and K2-P's genetic distance for 76 protein-coding genes showed that genes with evolutionary divergence might suffer from the effect of sequence regional constraints or gene functional constraints in Triticeae species. Our research will generally contribute to the knowledge of plastid genome evolution in Triticeae.
[Mh] Termos MeSH primário: Agropyron/genética
DNA de Cloroplastos/genética
Genes de Cloroplastos
Marcadores Genéticos
Variação Genética
Genoma de Cloroplastos
[Mh] Termos MeSH secundário: Agropyron/crescimento & desenvolvimento
Sequência de Bases
Evolução Molecular
Filogenia
Sequências Repetitivas de Ácido Nucleico
Análise de Sequência de DNA/métodos
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (Genetic Markers)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171015
[St] Status:MEDLINE


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[PMID]:28671248
[Au] Autor:Ali MA; Lee J; Al-Hemaid F
[Ad] Endereço:Department of Botany and Microbiology, College of Science, , , Saudi Arabia alimohammad@ksu.edu.sa.
[Ti] Título:Generic relationships among Molluginaceae inferred from a molecular phylogenetic analysis of the matK gene.
[So] Source:Genet Mol Res;16(2), 2017 Jun 29.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The family Molluginaceae (order Caryophyllales) is considered polyphyletic based on the photosynthetic pathway, C evolution, and phylogeny of the family. This inference was made based on photosynthetic, anatomical, and molecular datasets. The generic circumscription of this family has greatly been changed owing to the placement of several of its genera into the Caryophyllaceae, Microteaceae, Lophiocarpaceae, and Limeaceae families. However, the generic relationships are largely unknown. By virtue of high substitution rates within the species and the ability to resolve the phylogenetic position of morphologically very closely related species and species complexes, the matK gene has emerged as one of the potential chloroplast DNA molecular markers in plant molecular phylogenetics and DNA barcoding studies. We herein used molecular phylogenetic analyses of matK gene sequences using maximum parsimony and maximum likelihood analyses to infer the generic relationships among currently recognized genera circumscribed under the family Molluginaceae. The resulting phylogenetic tree confirmed the polyphyly of the family Molluginaceae. The genus Hypertelis was found at the base of the Molluginaceae clade. The genus Glinus was close to Glischrothamnus and Mollugo, Suessenguthiella was close to Coelanthum and Pharnaceum, whereas Polpoda grouped with Adenogramma and Psammotropha. The present study constitutes a robust investigation of the molecular phylogenetic relationships among members of the family Molluginaceae. Future study should combine by combined analyses of morphological characters and multiple nuclear and chloroplast DNA sequences with a more comprehensive taxon sampling of the family Molluginaceae.
[Mh] Termos MeSH primário: Genes de Cloroplastos
Molluginaceae/genética
Filogenia
[Mh] Termos MeSH secundário: Molluginaceae/classificação
Polimorfismo Genético
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029295


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[PMID]:28271308
[Au] Autor:Hens H; Jäkäläniemi A; Tali K; Efimov P; Kravchenko AV; Kvist L
[Ad] Endereço:Department of Ecology and Genetics, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland. hilde.hens@oulu.fi.
[Ti] Título:Genetic structure of a regionally endangered orchid, the dark red helleborine (Epipactis atrorubens) at the edge of its distribution.
[So] Source:Genetica;145(2):209-221, 2017 Apr.
[Is] ISSN:1573-6857
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The genetic structure and diversity of species is determined by both current population dynamics and historical processes. Population genetic structure at the edge of the distribution is often expected to differ substantially from populations at the centre, as these edge populations are often small and fragmented. In addition, populations located in regions that have experienced repeated glaciations throughout the Pleistocene, may still carry imprints from the genetic consequences of frequent distribution shifts. Using chloroplast DNA sequences and nuclear microsatellite markers we studied the genetic structure of Epipactis atrorubens at the northern edge of its distribution. Contrary to populations in the centre of the distribution, populations at the northern range are regionally endangered as they are small and disjunct. Sequence data of 2 chloroplast loci and allelic data from 6 nuclear microsatellite markers were obtained from 297 samples from Finland, Estonia and Russia. We sought for genetic indicators of past population processes, such as post-glacial colonisation history of E. atrorubens. As expected, we observed low genetic variation, in terms of numbers of substitutions, haplotypes and alleles, and significant levels of differentiation, especially pronounced in the chloroplast DNA. These features suggest that the edge populations could be prone to extinction.
[Mh] Termos MeSH primário: DNA de Cloroplastos/genética
Genes de Cloroplastos/genética
Repetições de Microssatélites/genética
Orchidaceae/genética
[Mh] Termos MeSH secundário: Animais
Ásia
Núcleo Celular/genética
DNA de Cloroplastos/química
Espécies em Perigo de Extinção
Europa (Continente)
Frequência do Gene
Variação Genética
Genética Populacional
Geografia
Haplótipos
Orchidaceae/classificação
Filogenia
Dinâmica Populacional
Federação Russa
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1007/s10709-017-9959-9


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[PMID]:28185042
[Au] Autor:Machado LO; Vieira LD; Stefenon VM; Oliveira Pedrosa F; Souza EM; Guerra MP; Nodari RO
[Ad] Endereço:Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
[Ti] Título:Phylogenomic relationship of feijoa (Acca sellowiana (O.Berg) Burret) with other Myrtaceae based on complete chloroplast genome sequences.
[So] Source:Genetica;145(2):163-174, 2017 Apr.
[Is] ISSN:1573-6857
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Given their distribution, importance, and richness, Myrtaceae species comprise a model system for studying the evolution of tropical plant diversity. In addition, chloroplast (cp) genome sequencing is an efficient tool for phylogenetic relationship studies. Feijoa [Acca sellowiana (O. Berg) Burret; CN: pineapple-guava] is a Myrtaceae species that occurs naturally in southern Brazil and northern Uruguay. Feijoa is known for its exquisite perfume and flavorful fruits, pharmacological properties, ornamental value and increasing economic relevance. In the present work, we reported the complete cp genome of feijoa. The feijoa cp genome is a circular molecule of 159,370 bp with a quadripartite structure containing two single copy regions, a Large Single Copy region (LSC 88,028 bp) and a Small Single Copy region (SSC 18,598 bp) separated by Inverted Repeat regions (IRs 26,372 bp). The genome structure, gene order, GC content and codon usage are similar to those of typical angiosperm cp genomes. When compared to other cp genome sequences of Myrtaceae, feijoa showed closest relationship with pitanga (Eugenia uniflora L.). Furthermore, a comparison of pitanga synonymous (Ks) and nonsynonymous (Ka) substitution rates revealed extremely low values. Maximum Likelihood and Bayesian Inference analyses produced phylogenomic trees identical in topology. These trees supported monophyly of three Myrtoideae clades.
[Mh] Termos MeSH primário: DNA Circular/genética
Feijoa/genética
Genoma de Cloroplastos/genética
Myrtaceae/genética
[Mh] Termos MeSH secundário: Composição de Bases/genética
Teorema de Bayes
Brasil
Códon/genética
DNA Circular/química
Feijoa/classificação
Ordem dos Genes
Genes de Cloroplastos/genética
Cadeias de Markov
Método de Monte Carlo
Mutação
Myrtaceae/classificação
Filogenia
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (DNA, Circular)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1007/s10709-017-9954-1


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[PMID]:28158776
[Au] Autor:Du L; Zhang J; Qu S; Zhao Y; Su B; Lv X; Li R; Wan Y; Xiao J
[Ad] Endereço:College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
[Ti] Título:The Pentratricopeptide Repeat Protein Pigment-Defective Mutant2 is Involved in the Regulation of Chloroplast Development and Chloroplast Gene Expression in Arabidopsis.
[So] Source:Plant Cell Physiol;58(4):747-759, 2017 04 01.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The development of functional chloroplasts, which is assisted by a series of nuclear-encoded auxiliary protein factors, is essential for plant autotrophic growth and development. To understand the molecular mechanisms underlying chloroplast development, we isolated and characterized a pigment-defective mutant, pdm2, and its corresponding variegated RNA interference (RNAi) lines in Arabidopsis. Sequence analysis revealed that PDM2 encodes a pentatricopeptide repeat protein that belongs to the P subgroup. Confocal microscopic analysis and immunoblotting of the chloroplast protein fraction showed that PDM2 was located in the stroma. In RNAi plants, protein-related photosynthesis was severely compromised. Furthermore, analysis of the transcript profile of chloroplast genes revealed that plastid-encoded polymerase-dependent transcript levels were markedly reduced, while nuclear-encoded polymerase-dependent transcript levels were increased, in RNAi plants. In addition, PDM2 affects plastid RNA editing efficiency in most editing sites, apparently by directly interacting with multiple organellar RNA editing factor 2 (MORF2) and MORF9. Thus, our results demonstrate that PDM2 is probably involved in the regulation of plastid gene expression required for normal chloroplast development.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/fisiologia
Cloroplastos/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Cloroplastos/metabolismo
Regulação da Expressão Gênica de Plantas
Técnicas de Silenciamento de Genes
Genes de Cloroplastos
Mutação
Plantas Geneticamente Modificadas
Plastídeos/genética
Edição de RNA
Sementes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (PDM2 protein, Arabidopsis)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1093/pcp/pcx004


  7 / 124 MEDLINE  
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[PMID]:28150126
[Au] Autor:Danilova MN; Kudryakova NV; Doroshenko AS; Zabrodin DA; Rakhmankulova ZF; Oelmüller R; Kusnetsov VV
[Ad] Endereço:Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow, 127276, Russia.
[Ti] Título:Opposite roles of the Arabidopsis cytokinin receptors AHK2 and AHK3 in the expression of plastid genes and genes for the plastid transcriptional machinery during senescence.
[So] Source:Plant Mol Biol;93(4-5):533-546, 2017 Mar.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Cytokinin membrane receptors of the Arabidopsis thaliana AHK2 and AHK3 play opposite roles in the expression of plastid genes and genes for the plastid transcriptional machinery during leaf senescence Loss-of-function mutants of Arabidopsis thaliana were used to study the role of cytokinin receptors in the expression of chloroplast genes during leaf senescence. Accumulation of transcripts of several plastid-encoded genes is dependent on the АНК2/АНК3 receptor combination. АНК2 is particularly important at the final stage of plant development and, unlike АНК3, a positive regulator of leaf senescence. Cytokinin-dependent up-regulation of the nuclear encoded genes for chloroplast RNA polymerases RPOTp and RPOTmp suggests that the hormone controls plastid gene expression, at least in part, via the expression of nuclear genes for the plastid transcription machinery. This is further supported by cytokinin dependent regulation of genes for the nuclear encoded plastid σ-factors, SIG1-6, which code for components of the transcriptional apparatus in chloroplasts.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Histidina Quinase/genética
Plastídeos/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Arabidopsis/fisiologia
Núcleo Celular/genética
Citocininas/metabolismo
Flores/genética
Flores/fisiologia
Genes de Cloroplastos/genética
Mutação
Folhas de Planta/genética
Folhas de Planta/fisiologia
Receptores de Superfície Celular/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Plântulas/genética
Plântulas/fisiologia
Sementes/genética
Sementes/fisiologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Receptors, Cell Surface); EC 2.7.13.1 (Histidine Kinase); EC 2.7.13.3 (AHK2 protein, Arabidopsis); EC 2.7.13.3 (AHK3 protein, Arabidopsis)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-016-0580-6


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[PMID]:28078742
[Au] Autor:Kawai H; Hanyuda T; Gao X; Terauchi M; Miyata M; Lindstrom SC; Klochkova NG; Miller KA
[Ad] Endereço:Kobe University Research Center for Inland Seas, 1-1 Rokkodai, Kobe, 657-8501, Japan.
[Ti] Título:Taxonomic revision of the Agaraceae with a description of Neoagarum gen. nov. and reinstatement of Thalassiophyllum.
[So] Source:J Phycol;53(2):261-270, 2017 Apr.
[Is] ISSN:1529-8817
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We confirmed the monophyly of the Agaraceae based on phylogenetic analyses of six mitochondrial and six chloroplast gene sequences from Agarum, Costaria, Dictyoneurum, and Thalassiophyllum species, as well as representative species from other laminarialean families. However, the genus Agarum was paraphyletic, comprising two independent clades, A. clathratum/A. turneri and A. fimbriatum/A. oharaense. The latter clade was genetically most closely related to Dictyoneurum spp., and morphologically, the species shared a flattened stipe bearing fimbriae (potential secondary haptera) in the mid- to upper portion. The phylogenetic position of Thalassiophyllum differed between the two datasets: in the chloroplast gene phylogeny, Thalassiophyllum was included in the A. clathratum/A. turneri clade, but in the mitochondrial gene phylogeny, it formed an independent clade at the base of the Agaraceae, the same position it took in the phylogeny when the data from both genomes were combined despite a larger number of bp being contributed by the chloroplast gene sequences. Considering the remarkable morphological differences between Thalassiophyllum and other Agaraceae, and the molecular support, we conclude that Thalassiophyllum should be reinstated as an independent genus. Dictyoneurum reticulatum was morphologically distinguishable from D. californicum due to its midrib, but because of their close genetic relationship, further investigations are needed to clarify species-level taxonomy. In summary, we propose the establishment of a new genus Neoagarum to accommodate A. fimbriatum and A. oharanese and the reinstatement of the genus Thalassiophyllum.
[Mh] Termos MeSH primário: Feófitas/genética
[Mh] Termos MeSH secundário: Genes de Cloroplastos/genética
Genes Mitocondriais/genética
Feófitas/classificação
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1111/jpy.12511


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[PMID]:28046132
[Au] Autor:Ma X; Han N; Shao C; Meng Y
[Ad] Endereço:College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, PR China.
[Ti] Título:Transcriptome-Wide Discovery of PASRs (Promoter-Associated Small RNAs) and TASRs (Terminus-Associated Small RNAs) in Arabidopsis thaliana.
[So] Source:PLoS One;12(1):e0169212, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hints from animals point to the existence of two novel small RNA (sRNA) species surrounding the transcription start sites (TSSs) and the termini of the genes, respectively. In this study, we performed a comprehensive search for the two sRNA species named promoter-associated sRNAs (PASRs) and terminus-associated sRNAs (TASRs) in Arabidopsis. By using sRNA sequencing data from wild type plants and several mutants related to the sRNA biogenesis, Argonaute (AGO) 1- and AGO4-associated sRNA sequencing data, double-stranded RNA sequencing (dsRNA-seq) data, and DNA methylation profiling data, the biogenesis and action pathways of the PASRs and the TASRs were investigated. PASR and TASR peaks were identified on hundreds of the protein-coding genes. Deep analysis uncovered that some of the sRNA peaks were covered by dsRNA-seq reads, and these peaks were significantly repressed in specific mutants. Besides, certain PASRs and TASRs were preferentially recruited by AGO4, and site-specific DNA methylation signals encompassing the genomic loci of these sRNAs were also detected. Accordingly, we proposed a model that certain PASRs and TASRs were generated through a specific Pol IV-, RDR-, DCL-dependent pathway, and they were associated with AGO4 to perform site-specific DNA methylation on their host genes. The above results indicate the existence of PASRs and TASRs in plants. The proposed biogenesis pathway and action mode of the PASRs and TASRs could facilitate us to perform in-depth functional studies on these novel sRNA species.
[Mh] Termos MeSH primário: Arabidopsis/genética
Regiões Promotoras Genéticas/genética
RNA de Plantas/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Proteínas Argonauta/genética
Proteínas Argonauta/metabolismo
Metilação de DNA/genética
Genes de Cloroplastos/genética
Fases de Leitura Aberta/genética
RNA de Cadeia Dupla/genética
RNA de Cadeia Dupla/metabolismo
RNA de Plantas/metabolismo
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Argonaute Proteins); 0 (RNA, Double-Stranded); 0 (RNA, Plant)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169212


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[PMID]:27760300
[Au] Autor:Jeong J; Baek K; Kirst H; Melis A; Jin E
[Ad] Endereço:Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
[Ti] Título:Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii.
[So] Source:Biochim Biophys Acta;1858(1):45-55, 2017 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The Chlamydomonas reinhardtii truncated light-harvesting antenna 4 (tla4) DNA transposon mutant has a pale green phenotype, a lower chlorophyll (Chl) per cell and a higher Chl a/b ratio in comparison with the wild type. It required a higher light intensity for the saturation of photosynthesis and displayed a greater per chlorophyll light-saturated rate of oxygen evolution than the wild type. The Chl antenna size of the photosystems in the tla4 mutant was only about 65% of that measured in the wild type. Molecular genetic analysis revealed that a single plasmid DNA insertion disrupted two genes on chromosome 11 of the mutant. A complementation study identified the "chloroplast signal recognition particle 54" gene (CpSRP54), as the lesion causing the tla4 phenotype. Disruption of this gene resulted in partial failure to assemble and, therefore, lower levels of light-harvesting Chl-binding proteins in the C. reinhardtii thylakoids. A comparative in silico 3-D structure-modeling analysis revealed that the M-domain of the CpSRP54 of C. reinhardtii possesses a more extended finger loop structure, due to different amino acid composition, as compared to that of the Arabidopsis CpSRP54. The work demonstrated that CpSRP54 deletion in microalgae can serve to generate tla mutants with a markedly smaller photosystem Chl antenna size, improved solar energy conversion efficiency, and photosynthetic productivity in high-density cultures under bright sunlight conditions.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Chlamydomonas reinhardtii/metabolismo
Complexos de Proteínas Captadores de Luz/metabolismo
Fotossíntese/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Aminoácidos/genética
Aminoácidos/metabolismo
Proteínas de Arabidopsis/genética
Chlamydomonas reinhardtii/genética
Clorofila/genética
Clorofila/metabolismo
Cloroplastos/genética
Cloroplastos/metabolismo
Elementos de DNA Transponíveis/genética
Genes de Cloroplastos/genética
Luz
Complexos de Proteínas Captadores de Luz/genética
Microalgas/genética
Microalgas/metabolismo
Fenótipo
Fotossíntese/genética
Alinhamento de Sequência
Partícula de Reconhecimento de Sinal/genética
Partícula de Reconhecimento de Sinal/metabolismo
Tilacoides/genética
Tilacoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 0 (Arabidopsis Proteins); 0 (DNA Transposable Elements); 0 (Light-Harvesting Protein Complexes); 0 (Signal Recognition Particle); 1406-65-1 (Chlorophyll); 5712ZB110R (chlorophyll b); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE



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