Base de dados : MEDLINE
Pesquisa : G05.360.340.024.340.310 [Categoria DeCS]
Referências encontradas : 2791 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 280 ir para página                         

  1 / 2791 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29183946
[Au] Autor:Yamamoto K; Kimura A
[Ad] Endereço:Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), Mishima 411-8540, Japan.
[Ti] Título:An asymmetric attraction model for the diversity and robustness of cell arrangement in nematodes.
[So] Source:Development;144(23):4437-4449, 2017 Dec 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During early embryogenesis in animals, cells are arranged into a species-specific pattern in a robust manner. Diverse cell arrangement patterns are observed, even among close relatives. In the present study, we evaluated the mechanisms by which the diversity and robustness of cell arrangements are achieved in developing embryos. We successfully reproduced various patterns of cell arrangements observed in various nematode species in embryos by altering the eggshell shapes. The findings suggest that the observed diversity of cell arrangements can be explained by differences in the eggshell shape. Additionally, we found that the cell arrangement was robust against eggshell deformation. Computational modeling revealed that, in addition to repulsive forces, attractive forces are sufficient to achieve such robustness. The present model is also capable of simulating the effect of changing cell division orientation. Genetic perturbation experiments demonstrated that attractive forces derived from cell adhesion are necessary for the robustness. The proposed model accounts for both diversity and robustness of cell arrangements, and contributes to our understanding of how the diversity and robustness of cell arrangements are achieved in developing embryos.
[Mh] Termos MeSH primário: Caenorhabditis elegans/citologia
Caenorhabditis elegans/embriologia
Modelos Biológicos
Nematoides/citologia
Nematoides/embriologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Caderinas/antagonistas & inibidores
Caderinas/genética
Caderinas/fisiologia
Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/antagonistas & inibidores
Proteínas de Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/fisiologia
Divisão Celular
Simulação por Computador
Proteínas do Citoesqueleto/antagonistas & inibidores
Proteínas do Citoesqueleto/genética
Proteínas do Citoesqueleto/fisiologia
Desenvolvimento Embrionário
Técnicas de Silenciamento de Genes
Genes de Helmintos
Mutação
Interferência de RNA
Especificidade da Espécie
beta Catenina/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadherins); 0 (Caenorhabditis elegans Proteins); 0 (Cytoskeletal Proteins); 0 (HMP-2 protein, C elegans); 0 (beta Catenin); 0 (hmr-1 protein, C elegans)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1242/dev.154609


  2 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28972978
[Au] Autor:Knapp J; Damy S; Brillaud J; Tissot JD; Navion J; Mélior R; Afonso E; Hormaz V; Gottstein B; Umhang G; Casulli A; Dadeau F; Millon L; Raoul F
[Ad] Endereço:Laboratoire Chrono-environnement UMR CNRS 6249, University Bourgogne Franche-Comté, Besançon, France.
[Ti] Título:EWET: Data collection and interface for the genetic analysis of Echinococcus multilocularis based on EmsB microsatellite.
[So] Source:PLoS One;12(10):e0183849, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Evolution and dispersion history on Earth of organisms can best be studied through biological markers in molecular epidemiological studies. The biological diversity of the cestode Echinococcus multilocularis was investigated in different cladistic approaches. First the morphological aspects were explored in connection with its ecology. More recently, molecular aspects were investigated to better understand the nature of the variations observed among isolates. The study of the tandemly repeated multilocus microsatellite EmsB allowed us to attain a high genetic diversity level where other classic markers have failed. Since 2006, EmsB data have been collected on specimens from various endemic foci of the parasite in Europe (in historic and newly endemic areas), Asia (China, Japan and Kyrgyzstan), and North America (Canada and Alaska). Biological data on the isolates and metadata were also recorded (e.g. host, geographical location, EmsB analysis, citation in the literature). In order to make available the data set of 1,166 isolates from classic and aberrant domestic and wild animal hosts (larval lesions and adult worms) and from human origin, an open web access interface, developed in PHP, and connected to a PostgreSQL database, was developed in the EmsB Website for the Echinococcus Typing (EWET) project. It allows researchers to access data collection, perform genetic analyses online (e.g. defining the genetic distance between their own samples and the samples in the database), consult distribution maps of EmsB profiles, and record and share their new EmsB genotyping data. In order to standardize the EmsB analyses performed in the different laboratories throughout the world, a calibrator was developed. The final aim of this project was to gather and arrange available data to permit to better understand the dispersion and transmission patterns of the parasite among definitive and intermediate hosts, in order to organize control strategies on the ground.
[Mh] Termos MeSH primário: Echinococcus multilocularis/genética
Repetições de Microssatélites/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Calibragem
Bases de Dados Genéticas
Genes de Helmintos
Marcadores Genéticos
Variação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183849


  3 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28965946
[Au] Autor:Shintani T; Sakoguchi H; Yoshihara A; Izumori K; Sato M
[Ad] Endereço:Research & Development, Matsutani Chemical Industry Co Ltd, Itami, Hyogo 664-8508, Japan.
[Ti] Título:d-Allulose, a stereoisomer of d-fructose, extends Caenorhabditis elegans lifespan through a dietary restriction mechanism: A new candidate dietary restriction mimetic.
[So] Source:Biochem Biophys Res Commun;493(4):1528-1533, 2017 Dec 02.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dietary restriction (DR) is an effective intervention known to increase lifespan in a wide variety of organisms. DR also delays the onset of aging-associated diseases. DR mimetics, compounds that can mimic the effects of DR, have been intensively explored. d-Allulose (d-Alu), the C3-epimer of d-fructose, is a rare sugar that has various health benefits, including anti-hyperglycemia and anti-obesity effects. Here, we report that d-Alu increased the lifespan of Caenorhabditis elegans both under monoxenic and axenic culture conditions. d-Alu did not further extend the lifespan of the long-lived DR model eat-2 mutant, strongly indicating that the effect is related to DR. However, d-Alu did not reduce the food intake of wild-type C. elegans. To explore the mechanisms of the d-Alu longevity effect, we examined the lifespan of d-Alu-treated mutants deficient for nutrient sensing pathway-related genes daf-16, sir-2.1, aak-2, and skn-1. As a result, d-Alu increased the lifespan of the daf-16, sir-2.1, and skn-1 mutants, but not the aak-2 mutant, indicating that the lifespan extension was dependent on the energy sensor, AMP-activated protein kinase (AMPK). d-Alu also enhanced the mRNA expression and enzyme activities of superoxide dismutase (SOD) and catalase. From these findings, we conclude that d-Alu extends lifespan by increasing oxidative stress resistance through a DR mechanism, making it a candidate DR mimetic.
[Mh] Termos MeSH primário: Caenorhabditis elegans/efeitos dos fármacos
Restrição Calórica/métodos
Frutose/farmacologia
[Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/metabolismo
Animais
Materiais Biomiméticos/química
Materiais Biomiméticos/farmacologia
Caenorhabditis elegans/genética
Caenorhabditis elegans/fisiologia
Proteínas de Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/metabolismo
Catalase/genética
Catalase/metabolismo
Ingestão de Alimentos/efeitos dos fármacos
Ingestão de Alimentos/genética
Ingestão de Alimentos/fisiologia
Frutose/química
Genes de Helmintos
Longevidade/efeitos dos fármacos
Longevidade/genética
Longevidade/fisiologia
Mutação
Estresse Oxidativo/efeitos dos fármacos
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Receptores Nicotínicos/genética
Receptores Nicotínicos/metabolismo
Estereoisomerismo
Superóxido Dismutase/genética
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Eat-2 protein, C elegans); 0 (Receptors, Nicotinic); 23140-52-5 (psicose); 30237-26-4 (Fructose); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.31 (AAK-2 protein, C elegans); EC 2.7.11.31 (AMP-Activated Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


  4 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28655614
[Au] Autor:Dash M; Dutta TK; Phani V; Papolu PK; Shivakumara TN; Rao U
[Ad] Endereço:Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
[Ti] Título:RNAi-mediated disruption of neuropeptide genes, nlp-3 and nlp-12, cause multiple behavioral defects in Meloidogyne incognita.
[So] Source:Biochem Biophys Res Commun;490(3):933-940, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Owing to the current deficiencies in chemical control options and unavailability of novel management strategies, root-knot nematode (M. incognita) infections remain widespread with significant socio-economic impacts. Helminth nervous systems are peptide-rich and appear to be putative drug targets that could be exploited by antihelmintic chemotherapy. Herein, to characterize the novel peptidergic neurotransmitters, in silico mining of M. incognita genomic and transciptomic datasets revealed the presence of 16 neuropeptide-like protein (nlp) genes with structural hallmarks of neuropeptide preproproteins; among which 13 nlps were PCR-amplified and sequenced. Two key nlp genes (Mi-nlp-3 and Mi-nlp-12) were localized to the basal bulb and tail region of nematode body via in situ hybridization assay. Mi-nlp-3 and Mi-nlp-12 were greatly expressed (in qRT-PCR assay) in the pre-parasitic juveniles and adult females, suggesting the association of these genes in host recognition, development and reproduction of M. incognita. In vitro knockdown of Mi-nlp-3 and Mi-nlp-12 via RNAi demonstrated the significant reduction in attraction and penetration of M. incognita in tomato root in Pluronic gel medium. A pronounced perturbation in development and reproduction of NLP-silenced worms was also documented in adzuki beans in CYG growth pouches. The deleterious phenotypes obtained due to NLP knockdown suggests that transgenic plants engineered to express RNA constructs targeting nlp genes may emerge as an environmentally viable option to manage nematode problems in crop plants.
[Mh] Termos MeSH primário: Genes de Helmintos
Neuropeptídeos/genética
Doenças das Plantas/parasitologia
Plantas/parasitologia
Interferência de RNA
Infecções por Secernentea/parasitologia
Tylenchoidea/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Genômica
Neuropeptídeos/análise
Neuropeptídeos/metabolismo
Tylenchoidea/química
Tylenchoidea/fisiologia
Tylenchoidea/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuropeptides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


  5 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28648843
[Au] Autor:Linden LM; Gordon KL; Pani AM; Payne SG; Garde A; Burkholder D; Chi Q; Goldstein B; Sherwood DR
[Ad] Endereço:Department of Biology, Regeneration Next, Duke University, Durham, NC 27708, USA.
[Ti] Título:Identification of regulators of germ stem cell enwrapment by its niche in C. elegans.
[So] Source:Dev Biol;429(1):271-284, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many stem cell niches contain support cells that increase contact with stem cells by enwrapping them in cellular processes. One example is the germ stem cell niche in C. elegans, which is composed of a single niche cell termed the distal tip cell (DTC) that extends cellular processes, constructing an elaborate plexus that enwraps germ stem cells. To identify genes required for plexus formation and to explore the function of this specialized enwrapping behavior, a series of targeted and tissue-specific RNAi screens were performed. Here we identify genes that promote stem cell enwrapment by the DTC plexus, including a set that specifically functions within the DTC, such as the chromatin modifier lin-40/MTA1, and others that act within the germline, such as the 14-3-3 signaling protein par-5. Analysis of genes that function within the germline to mediate plexus development reveal that they are required for expansion of the germ progenitor zone, supporting the emerging idea that germ stem cells signal to the niche to stimulate enwrapping behavior. Examination of wild-type animals with asymmetric plexus formation and animals with reduced DTC plexus elaboration via loss of two candidates including lin-40 indicate that cellular enwrapment promotes GLP-1/Notch signaling and germ stem cell fate. Together, our work identifies novel regulators of cellular enwrapment and suggests that reciprocal signaling between the DTC niche and the germ stem cells promotes enwrapment behavior and stem cell fate.
[Mh] Termos MeSH primário: Caenorhabditis elegans/citologia
Células Germinativas/citologia
Nicho de Células-Tronco
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/embriologia
Caenorhabditis elegans/genética
Linhagem da Célula
Embrião não Mamífero/citologia
Genes de Helmintos
Genes Reporter
Células Germinativas/metabolismo
Interferência de RNA
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


  6 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28634272
[Au] Autor:Schultz J; Lee SJ; Cole T; Hoang HD; Vibbert J; Cottee PA; Miller MA; Han SM
[Ad] Endereço:Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
[Ti] Título:The secreted MSP domain of VAPB homolog VPR-1 patterns the adult striated muscle mitochondrial reticulum via SMN-1.
[So] Source:Development;144(12):2175-2186, 2017 06 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The major sperm protein domain (MSPd) has an extracellular signaling function implicated in amyotrophic lateral sclerosis. Secreted MSPds derived from the VAPB homolog VPR-1 promote mitochondrial localization to actin-rich I-bands in body wall muscle. Here we show that the nervous system and germ line are key MSPd secretion tissues. MSPd signals are transduced through the CLR-1 Lar-like tyrosine phosphatase receptor. We show that CLR-1 is expressed throughout the muscle plasma membrane, where it is accessible to MSPd within the pseudocoelomic fluid. MSPd signaling is sufficient to remodel the muscle mitochondrial reticulum during adulthood. An RNAi suppressor screen identified survival of motor neuron 1 (SMN-1) as a downstream effector. SMN-1 acts in muscle, where it colocalizes at myofilaments with ARX-2, a component of the Arp2/3 actin-nucleation complex. Genetic studies suggest that SMN-1 promotes Arp2/3 activity important for localizing mitochondria to I-bands. Our results support the model that VAPB homologs are circulating hormones that pattern the striated muscle mitochondrial reticulum. This function is crucial in adults and requires SMN-1 in muscle, likely independent of its role in pre-mRNA splicing.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/metabolismo
Proteínas de Membrana/metabolismo
Músculo Estriado/crescimento & desenvolvimento
Músculo Estriado/metabolismo
Proteínas do Complexo SMN/metabolismo
[Mh] Termos MeSH secundário: Proteína 2 Relacionada a Actina/metabolismo
Complexo 2-3 de Proteínas Relacionadas à Actina/metabolismo
Esclerose Amiotrófica Lateral/metabolismo
Animais
Animais Geneticamente Modificados
Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/química
Proteínas de Caenorhabditis elegans/genética
Genes de Helmintos
Células Germinativas/metabolismo
Seres Humanos
Larva/crescimento & desenvolvimento
Larva/metabolismo
Masculino
Proteínas de Membrana/química
Proteínas de Membrana/genética
Mitocôndrias Musculares/metabolismo
Neurônios Motores/metabolismo
Mutação
Domínios Proteicos
Interferência de RNA
Proteínas Tirosina Fosfatases Semelhantes a Receptores/genética
Proteínas Tirosina Fosfatases Semelhantes a Receptores/metabolismo
Proteínas do Complexo SMN/antagonistas & inibidores
Proteínas do Complexo SMN/genética
Sarcolema/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Actin-Related Protein 2); 0 (Actin-Related Protein 2-3 Complex); 0 (Caenorhabditis elegans Proteins); 0 (Membrane Proteins); 0 (SMN Complex Proteins); 0 (VPR-1 protein, C elegans); 0 (arx-2 protein, C elegans); EC 3.1.3.48 (CLR-1 protein, C elegans); EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1242/dev.152025


  7 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28619826
[Au] Autor:Wang S; Tang NH; Lara-Gonzalez P; Zhao Z; Cheerambathur DK; Prevo B; Chisholm AD; Desai A; Oegema K
[Ad] Endereço:Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA.
[Ti] Título:A toolkit for GFP-mediated tissue-specific protein degradation in .
[So] Source:Development;144(14):2694-2701, 2017 07 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Proteins that are essential for embryo production, cell division and early embryonic events are frequently reused later in embryogenesis, during organismal development or in the adult. Examining protein function across these different biological contexts requires tissue-specific perturbation. Here, we describe a method that uses expression of a fusion between a GFP-targeting nanobody and a SOCS-box containing ubiquitin ligase adaptor to target GFP-tagged proteins for degradation. When combined with endogenous locus GFP tagging by CRISPR-Cas9 or with rescue of a null mutant with a GFP fusion, this approach enables routine and efficient tissue-specific protein ablation. We show that this approach works in multiple tissues - the epidermis, intestine, body wall muscle, ciliated sensory neurons and touch receptor neurons - where it recapitulates expected loss-of-function mutant phenotypes. The transgene toolkit and the strain set described here will complement existing approaches to enable routine analysis of the tissue-specific roles of proteins.
[Mh] Termos MeSH primário: Caenorhabditis elegans/metabolismo
Proteínas de Fluorescência Verde/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Axônios/metabolismo
Caenorhabditis elegans/genética
Caenorhabditis elegans/crescimento & desenvolvimento
Proteínas de Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/metabolismo
Proteínas de Transporte/genética
Proteínas de Transporte/metabolismo
Genes de Helmintos
Técnicas Genéticas
Proteínas de Fluorescência Verde/genética
MAP Quinase Quinase Quinases/genética
MAP Quinase Quinase Quinases/metabolismo
Mutação
Proteólise
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Anticorpos de Domínio Único/genética
Anticorpos de Domínio Único/metabolismo
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Carrier Proteins); 0 (Recombinant Fusion Proteins); 0 (Single-Domain Antibodies); 0 (ZIF-1 protein, C elegans); 147336-22-9 (Green Fluorescent Proteins); EC 2.7.11.25 (DLK-1 protein, C elegans); EC 2.7.11.25 (MAP Kinase Kinase Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1242/dev.150094


  8 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28490217
[Au] Autor:Ren M; Zhao L; Lv X; Wang D
[Ad] Endereço:a Key Laboratory of Environmental Medicine Engineering in Ministry of Education , Medical School, Southeast University , Nanjing , China.
[Ti] Título:Antimicrobial proteins in the response to graphene oxide in Caenorhabditis elegans.
[So] Source:Nanotoxicology;11(4):578-590, 2017 May.
[Is] ISSN:1743-5404
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Upon exposure to environmental engineered nanomaterials (ENMs), animals will activate certain response signals to protect themselves from the toxic effects. However, the underlying molecular mechanisms for this response are still largely unclear. Using in vivo assay system of Caenorhabditis elegans, we here found that antimicrobial proteins of LYS-1, LYS-8, SPP-1, DOD-6, and F55G11.4 were activated by graphene oxide (GO) exposure. These antimicrobial proteins functioned as molecular targets of transcriptional factor DAF-16 in insulin signaling pathway, and acted in intestine to regulate the response to GO. Among these antimicrobial proteins, DOD-6, F55G11.4, and SPP-1 participated in the formation of signaling cascade of DAF-16-DOD-6-SOD-3-F55G11.4/SPP-1 in response to GO exposure by activating the antioxidation system. Different from this, LYS-1 and LYS-8, two lysozymes, mediated TUB-2 signaling and DAF-8-DAF-5 signaling cascade, respectively, to regulate the response to GO exposure. During the regulation of response to GO exposure, LYS-1 and LYS-8 acted synergistically, which could be largely explained by the observed synergistic interaction between TUB-2 and DAF-8. Therefore, our results demonstrate the crucial protection role of antimicrobial proteins for animals in response to environmental ENMs' exposure. The elucidated different signaling cascades mediated by antimicrobial proteins provide important molecular targets for future toxicity assessment and chemical modification of GO.
[Mh] Termos MeSH primário: Anti-Infecciosos/metabolismo
Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/efeitos dos fármacos
Grafite/toxicidade
Insulina/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/genética
Caenorhabditis elegans/metabolismo
Proteínas de Caenorhabditis elegans/genética
Genes de Helmintos
Insulina/genética
Óxidos/toxicidade
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Caenorhabditis elegans Proteins); 0 (Insulin); 0 (Oxides); 7782-42-5 (Graphite)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1080/17435390.2017.1329954


  9 / 2791 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28253355
[Au] Autor:Morris R; Wilson L; Sturrock M; Warnock ND; Carrizo D; Cox D; Maule AG; Dalzell JJ
[Ad] Endereço:School of Biological Sciences, Institute for Global Food Security, Queen's University Belfast, Belfast, United Kingdom.
[Ti] Título:A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae.
[So] Source:PLoS Pathog;13(3):e1006185, 2017 Mar.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Entomopathogenic nematodes (EPNs) employ a sophisticated chemosensory apparatus to detect potential hosts. Understanding the molecular basis of relevant host-finding behaviours could facilitate improved EPN biocontrol approaches, and could lend insight to similar behaviours in economically important mammalian parasites. FMRFamide-like peptides are enriched and conserved across the Phylum Nematoda, and have been linked with motor and sensory function, including dispersal and aggregating behaviours in the free living nematode Caenorhabditis elegans. The RNA interference (RNAi) pathway of Steinernema carpocapsae was characterised in silico, and employed to knockdown the expression of the FMRFamide-like peptide 21 (GLGPRPLRFamide) gene (flp-21) in S. carpocapsae infective juveniles; a first instance of RNAi in this genus, and a first in an infective juvenile of any EPN species. Our data show that 5 mg/ml dsRNA and 50 mM serotonin triggers statistically significant flp-21 knockdown (-84%***) over a 48 h timecourse, which inhibits host-finding (chemosensory), dispersal, hyperactive nictation and jumping behaviours. However, whilst 1 mg/ml dsRNA and 50 mM serotonin also triggers statistically significant flp-21 knockdown (-51%**) over a 48 h timecourse, it does not trigger the null sensory phenotypes; statistically significant target knockdown can still lead to false negative results, necessitating appropriate experimental design. SPME GC-MS volatile profiles of two EPN hosts, Galleria mellonella and Tenebrio molitor reveal an array of shared and unique compounds; these differences had no impact on null flp-21 RNAi phenotypes for the behaviours assayed. Localisation of flp-21 / FLP-21 to paired anterior neurons by whole mount in situ hybridisation and immunocytochemistry corroborates the RNAi data, further suggesting a role in sensory modulation. These data can underpin efforts to study these behaviours in other economically important parasites, and could facilitate molecular approaches to EPN strain improvement for biocontrol.
[Mh] Termos MeSH primário: Técnicas de Silenciamento de Genes/métodos
Interações Hospedeiro-Parasita/fisiologia
Nematoides/fisiologia
Neuropeptídeos/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromatografia Gasosa
Genes de Helmintos
Imuno-Histoquímica
Hibridização In Situ
Espectrometria de Massas
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuropeptides)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006185


  10 / 2791 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28212676
[Au] Autor:Li Y; Yin M; Wu Q; McManus DP; Blair D; Li H; Xu B; Mo X; Feng Z; Hu W
[Ad] Endereço:School of Life Science, Fudan University, Songhu Road 2005, Shanghai, China.
[Ti] Título:Genetic diversity and selection of three nuclear genes in Schistosoma japonicum populations.
[So] Source:Parasit Vectors;10(1):87, 2017 Feb 17.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The blood fluke, Schistosoma japonicum still causes severe disease in China, the Philippines and Indonesia. Although there have been some studies the molecular epidemiology of this persistent and harmful parasite, few have explored the possibility and implications of selection in S. japonicum populations. METHODS: We analyzed diversity and looked for evidence of selection at three nuclear genes (SjIpp2, SjFabp and SjT22.6) in 13 S. japonicum populations. RESULTS: SjT22.6 was found to exhibit high nucleotide diversity and was under positive selection in the mountainous region of mainland China. As a tegumental protein, its secondary and tertiary structure differed between S. japonicum strains from the mountainous and lakes regions. In contrast, SjIpp2 and SjFabp had relatively low levels of nucleotide diversity and did not show significant departure from neutrality. CONCLUSIONS: As a tegument-associated antigen-encoding gene of S. japonicum, SjT22.6 has high nucleotide diversity and appears to be under positive selection in the mountainous region of mainland China.
[Mh] Termos MeSH primário: Genes de Helmintos
Variação Genética
Schistosoma japonicum/genética
Seleção Genética
[Mh] Termos MeSH secundário: Animais
Antígenos de Helmintos/genética
China
Esquistossomose Japônica/parasitologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2033-8



página 1 de 280 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde