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Pesquisa : G05.360.340.024.340.364.124 [Categoria DeCS]
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  1 / 1079 MEDLINE  
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[PMID]:27771305
[Au] Autor:Walter ME; Ortiz A; Sondgeroth C; Sindt NM; Duszenko N; Catlett JL; Zhou Y; Valloppilly S; Anderson C; Fernando S; Buan NR
[Ad] Endereço:Redox Biology Center, Department of Biochemistry, University of Nebraska-Lincoln, N200 Beadle Center, Lincoln, NE 68588-0664, United States.
[Ti] Título:High-throughput mutation, selection, and phenotype screening of mutant methanogenic archaea.
[So] Source:J Microbiol Methods;131:113-121, 2016 12.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bacterial and archaeal genomes can contain 30% or more hypothetical genes with no predicted function. Phylogenetically deep-branching microbes, such as methane-producing archaea (methanogens), contain up to 50% genes with unknown function. In order to formulate hypotheses about the function of hypothetical gene functions in the strict anaerobe, Methanosarcina acetivorans, we have developed high-throughput anaerobic techniques to UV mutagenize, screen, and select for mutant strains in 96-well plates. Using these approaches we have isolated 10 mutant strains that exhibit a variety of physiological changes including increased or decreased growth rate relative to the parent strain when cells use methanol and/or acetate as carbon and energy sources. This method provides an avenue for the first step in identifying new gene functions: associating a genetic mutation with a reproducible phenotype. Mutations in bona fide methanogenesis genes such as corrinoid methyltransferases and proton-translocating F H :methanophenazine oxidoreductase (Fpo) were also generated, opening the door to in vivo functional complementation experiments. Irradiation-based mutagenesis such as from ultraviolet (UV) light, combined with modern genome sequencing, is a useful procedure to discern systems-level gene function in prokaryote taxa that can be axenically cultured but which may be resistant to chemical mutagens.
[Mh] Termos MeSH primário: Archaea/genética
Archaea/isolamento & purificação
Archaea/efeitos da radiação
Ensaios de Triagem em Larga Escala/métodos
Fenótipo
Mutação Puntual/efeitos da radiação
Raios Ultravioleta
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Archaea/metabolismo
DNA Arqueal/genética
DNA Arqueal/efeitos da radiação
Genes Arqueais
Metano/metabolismo
Metanol/metabolismo
Methanosarcina/genética
Methanosarcina/crescimento & desenvolvimento
Methanosarcina/efeitos da radiação
Metiltransferases/genética
Viabilidade Microbiana/efeitos da radiação
Mutagênese/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (DNA, Archaeal); EC 2.1.1.- (Methyltransferases); OP0UW79H66 (Methane); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  2 / 1079 MEDLINE  
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[PMID]:28905703
[Au] Autor:Yin XQ; Liu BB; Chu X; Salam N; Li X; Yang ZW; Zhang Y; Xiao M; Li WJ
[Ad] Endereço:1​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
[Ti] Título:Saliphagus infecundisoli gen. nov., sp. nov., an extremely halophilic archaeon isolated from a saline soil.
[So] Source:Int J Syst Evol Microbiol;67(10):4154-4160, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two extremely halophilic archaea, strains YIM 93745 and YIM 93707, were isolated from a saline soil sample collected from Loulan, China. Cells of the two strains were coccus, non-motile and Gram-stain negative. The strains were aerobic and grew at 25-50 °C (optimum, 37 °C), in the presence of 5-35 % (w/v) NaCl (optimum, 20 %), 0.01-0.1 M Mg (optimum, 0.03 M) and pH 6.0-8.5 (optimum, 7.0-7.5). Cells lysed in distilled water and with 0-5 % NaCl. Major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glycosyl diether and two unidentified glycolipids. Phylogenetic analysis based on the 16S rRNA sequence revealed that the two strains were most closely related to Halovivax cerinus IC35 (95.1 and 95.2 % sequence similarities, respectively). The two strains, however, shared highest rpoB' gene sequence identities with Natrinema pellirubrum JCM 10476 (87.8 and 87.7 % respectively). Phylogenetic trees based on 16S rRNA and rpoB' gene sequences demonstrated a robust clade of the two strains with members of related genera of the family Natrialbaceae. The DNA G+C contents of the two strains were 64.6 and 64.4 mol%, respectively. DNA-DNA relatedness values between them were 95±2 %. Phenotypic, chemotaxonomic characteristics and phylogenetic properties suggested that the two strains YIM 93745 and YIM 93707 represent a novel species in a new genus within the family Natrialbaceae, for which the name Saliphagus infecundisoli gen. nov., sp. nov. is proposed. The type strain is YIM 93745 (=KCTC 4228 =CGMCC 1.15824 ).
[Mh] Termos MeSH primário: Halobacteriaceae/classificação
Filogenia
Salinidade
Microbiologia do Solo
[Mh] Termos MeSH secundário: China
DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/química
Halobacteriaceae/genética
Halobacteriaceae/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002270


  3 / 1079 MEDLINE  
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[PMID]:28901903
[Au] Autor:Lü ZZ; Li Y; Zhou Y; Cui HL; Li ZR
[Ad] Endereço:1​School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
[Ti] Título:Halobacterium litoreum sp. nov., isolated from a marine solar saltern.
[So] Source:Int J Syst Evol Microbiol;67(10):4095-4099, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Halophilic archaeal strain ZS-54-S2 was isolated from Zhoushan marine solar saltern, China. Cells were rod-shaped, Gram-stain-negative and formed red-pigmented colonies on an agar plate. Strain ZS-54-S2 was able to grow at 20-50 °C (optimum 35 °C), at 1.7-4.8 M NaCl (optimum 3.9 M), at 0.005-1.0 M MgCl2 (optimum 0.05 M) and at pH 5.0-9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two glycolipids, which were chromatographically identical to sulfated galactosyl mannosyl galactofuranosyl glucosyl diether and galactosyl mannosyl glucosyl diether, and an unidentified glycolipid, which was chromatographically identical to one detected in Halobacterium salinarum ATCC 33171 . The 16S rRNA gene and rpoB' gene of strain ZS-54-S2 were phylogenetically related to the corresponding genes of Halobacterium noricense JCM 15102 (97.5 % and 90.6 % relatedness, respectively), Halobacterium jilantaiense CGMCC 1.5337 (96.9 and 91.2 %), Halobacterium rubrum CGMCC 1.12575 (96.8 and 90.3 %) and Halobacterium salinarum CGMCC 1.1958 (96.5 and 88.4 %). The DNA G+C content of strain ZS-54-S2 was 66.7 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-54-S2 (=CGMCC 1.12562 =JCM 30038 ) represents a new species of Halobacterium, for which the name Halobacteriumlitoreum sp. nov. is proposed.
[Mh] Termos MeSH primário: Halobacterium/classificação
Filogenia
Salinidade
Microbiologia da Água
[Mh] Termos MeSH secundário: Composição de Bases
China
DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/análise
Halobacterium/genética
Halobacterium/isolamento & purificação
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002259


  4 / 1079 MEDLINE  
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[PMID]:28875907
[Au] Autor:Cui HL; Lü ZZ; Li Y; Zhou Y
[Ad] Endereço:School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
[Ti] Título:Salinirussus salinus gen. nov., sp. nov., isolated from a marine solar saltern.
[So] Source:Int J Syst Evol Microbiol;67(9):3622-3626, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A halophilic archaeal strain, YGH44T, was isolated from the Yinggehai marine solar saltern in Hainan Province of China. Cells were rod-shaped, stained Gram-negative and formed red-pigmented colonies on agar plates. Optimal growth was obtained with 3.4 M NaCl (range: 2.6-4.8 M), 0.5 M MgCl2 (range: 0.005-1.0 M), at 37 °C (range: 25-55 °C) and at pH 7.0 (range: pH 5.0-9.0). The cells lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was 1.7 M. Phylogenetic tree reconstructions based on 16S rRNA genes and rpoB' genes revealed that strain YGH44T was distinct from the related genera, Halovenus, Halapricum, Halorientalis, Halorhabdus and Halosimplex of the order Halobacteriales. The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three unidentified glycolipids. The DNA G+C content of strain YGH44Twas 69.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YGH44T (=CGMCC 1.12234T=JCM 18646T) represents a novel species of a new genus within the order Halobacteriales, for which the name Salinirussus salinus gen. nov., sp. nov. is proposed.
[Mh] Termos MeSH primário: Halobacteriaceae/classificação
Filogenia
Salinidade
Microbiologia da Água
[Mh] Termos MeSH secundário: Composição de Bases
China
DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/química
Halobacteriaceae/genética
Halobacteriaceae/isolamento & purificação
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002182


  5 / 1079 MEDLINE  
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[PMID]:28840815
[Au] Autor:Naghoni A; Emtiazi G; Amoozegar MA; Rasooli M; Etemadifar Z; Shahzadeh Fazeli SA; Minegishi H; Ventosa A
[Ad] Endereço:1​Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.
[Ti] Título:Natronoarchaeum persicum sp. nov., a haloarchaeon isolated from a hypersaline lake.
[So] Source:Int J Syst Evol Microbiol;67(9):3339-3344, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37-40 °C; it was able to grow at pH 6.0-8.5 and 20-55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain WIIAL99T is a member of the genus Natronoarchaeum in the family Halobacteriaceae and forms a distinct cluster. It was concluded that strain WIIAL99T (=IBRC-M 11062T=LMG 29814T) represents a novel species of the genus Natronoarchaeum, for which the name Natronoarchaeum persicum sp. nov. is proposed.
[Mh] Termos MeSH primário: Halobacteriaceae/classificação
Filogenia
Salinidade
Microbiologia da Água
[Mh] Termos MeSH secundário: Composição de Bases
DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/química
Halobacteriaceae/genética
Halobacteriaceae/isolamento & purificação
Irã (Geográfico)
Lagos/microbiologia
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002116


  6 / 1079 MEDLINE  
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[PMID]:28650314
[Au] Autor:Rasooli M; Naghoni A; Amoozegar MA; Mirfeizi L; Moshtaghi Nikou M; Shahzadeh Fazeli SA; Minegishi H; Ventosa A
[Ad] Endereço:1​Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran.
[Ti] Título:Natrinema soli sp. nov., a novel halophilic archaeon isolated from a hypersaline wetland.
[So] Source:Int J Syst Evol Microbiol;67(7):2142-2147, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An extremely halophilic archaeon, designated strain 5-3T, was isolated from a soil sample of Meighan wetland in Iran. Strain 5-3T was strictly aerobic, catalase-positive and oxidase-negative. Cells were Gram-stain-negative, non-motile and ovoid. Colonies of strain 5-3T were cream-coloured. The isolate showed optimum growth at 4.0 M NaCl, 40 °C and pH 7.0. The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two unknown phospholipids and three glycolipids (including one that was chromatographically identical to S2-DGD). The major respiratory quinone was menaquinone MK-8. The G+C content of the genomic DNA was 61.5 mol%. The closest relative was Natrinema salaciae JCM 17869T with 97.3 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain 5-3T is a member of the genus Natrinema in the family Natrialbaceae and forms a distinct cluster. On the basis of phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, a novel species of the family Natrialbaceae, Natrinema soli sp. nov., is proposed. The type strain is 5-3T (=IBRC-M 11063T=LMG 29247T).
[Mh] Termos MeSH primário: Halobacteriaceae/classificação
Filogenia
Zonas Úmidas
[Mh] Termos MeSH secundário: DNA Arqueal/genética
Genes Arqueais
Glicolipídeos/química
Halobacteriaceae/genética
Halobacteriaceae/isolamento & purificação
Irã (Geográfico)
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Salinidade
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-38-6 (vitamin MK 8)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001909


  7 / 1079 MEDLINE  
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[PMID]:28344109
[Au] Autor:Bharathi M; Chellapandi P
[Ad] Endereço:Molecular Systems Engineering Lab., Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India.
[Ti] Título:Phylogenomic proximity and metabolic discrepancy of Methanosarcina mazei Go1 across methanosarcinal genomes.
[So] Source:Biosystems;155:20-28, 2017 May.
[Is] ISSN:1872-8324
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Methanosarcina mazei Go1 is a heterotrophic methanogenic archaean contributing a significant role in global methane cycling and biomethanation process. Phylogenomic relatedness and metabolic discrepancy of this genome were described herein by comparing its whole genome sequence, intergenomic distance, genome function, synteny homologs and origin of replication, and marker genes with very closely related genomes, Methanosarcina acetivorans and Methanosarcina barkeri. Phylogenomic analysis of this study revealed that genome functional feature and metabolic core of M. mazei and M. barkeri could be originated from M. acetivorans. The metabolic core of these genomes shares a common evolutionary origin to perform the metabolic activity at different environmental niches. Genome expansion, dynamics and gene collinearity were constrained and restrained the conservation of the metabolic core genes by duplication events occurring across methanosarcinal genomes. The Darwinian positive selection was an evolutionary constraint to purify the function of core metabolic genes. Using genome-wide metabolic survey, we found the existence of four novel putative metabolic pathways such as complete methanogenesis from acetate, indole-3-acetate biosynthesis V, 4-aminobutyrate degradation III, galactosamine biosynthesis I and siroheme biosynthesis. Overall, the present study would provide a stand point to revisit its phylogenomic status in order to understand the origin and evolution history of this organism.
[Mh] Termos MeSH primário: Genoma Arqueal/genética
Metano/metabolismo
Methanosarcina/genética
Methanosarcina/metabolismo
[Mh] Termos MeSH secundário: Proteínas Arqueais/genética
Proteínas Arqueais/metabolismo
Evolução Molecular
Galactosamina/biossíntese
Duplicação Gênica
Genes Arqueais/genética
Variação Genética
Heme/análogos & derivados
Heme/biossíntese
Ácidos Indolacéticos/metabolismo
Redes e Vias Metabólicas/genética
Methanosarcina/classificação
Filogenia
Especificidade da Espécie
Ácido gama-Aminobutírico/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Indoleacetic Acids); 42VZT0U6YR (Heme); 52553-42-1 (siroheme); 56-12-2 (gamma-Aminobutyric Acid); 6U1S09C61L (indoleacetic acid); 7535-00-4 (Galactosamine); OP0UW79H66 (Methane)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


  8 / 1079 MEDLINE  
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[PMID]:28334743
[Au] Autor:Nakagawa S; Niimura Y; Gojobori T
[Ad] Endereço:Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan.
[Ti] Título:Comparative genomic analysis of translation initiation mechanisms for genes lacking the Shine-Dalgarno sequence in prokaryotes.
[So] Source:Nucleic Acids Res;45(7):3922-3931, 2017 Apr 20.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In prokaryotes, translation initiation is believed to occur through an interaction between the 3΄ tail of a 16S rRNA and a corresponding Shine-Dalgarno (SD) sequence in the 5΄ untranslated region (UTR) of an mRNA. However, some genes lack SD sequences (non-SD genes), and the fraction of non-SD genes in a genome varies depending on the prokaryotic species. To elucidate non-SD translation initiation mechanisms in prokaryotes from an evolutionary perspective, we statistically examined the nucleotide frequencies around the initiation codons in non-SD genes from 260 prokaryotes (235 bacteria and 25 archaea). We identified distinct nucleotide frequency biases upstream of the initiation codon in bacteria and archaea, likely because of the presence of leaderless mRNAs lacking a 5΄ UTR. Moreover, we observed overall similarities in the nucleotide patterns between upstream and downstream regions of the initiation codon in all examined phyla. Symmetric nucleotide frequency biases might facilitate translation initiation by preventing the formation of secondary structures around the initiation codon. These features are more prominent in species' genomes that harbor large fractions of non-SD sequences, suggesting that a reduced stability around the initiation codon is important for efficient translation initiation in prokaryotes.
[Mh] Termos MeSH primário: Genes Arqueais
Genes Bacterianos
Iniciação Traducional da Cadeia Peptídica
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Códon de Iniciação
Genômica
Conformação de Ácido Nucleico
Nucleotídeos/análise
RNA Mensageiro/química
RNA Ribossômico 16S/química
Sequências Reguladoras de Ácido Ribonucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Codon, Initiator); 0 (Nucleotides); 0 (RNA, Messenger); 0 (RNA, Ribosomal, 16S); 0 (Regulatory Sequences, Ribonucleic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx124


  9 / 1079 MEDLINE  
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[PMID]:28126396
[Au] Autor:Huang Y; Ou D; Chen S; Chen B; Liu W; Bai R; Chen G
[Ad] Endereço:Marine Biology Institute, Shantou University, Shantou, Guangdong, China; Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, China.
[Ti] Título:Inhibition effect of zinc in wastewater on the N O emission from coastal loam soils.
[So] Source:Mar Pollut Bull;116(1-2):434-439, 2017 Mar 15.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of zinc (Zn) on nitrous oxide (N O) fluxes from coastal loam soil and the abundances of soil nitrifier and denitrifier were studied in a tidal microcosm receiving livestock wastewater with different Zn levels. Soil N O emission significantly increased due to discharge of wastewater rich in ammonia (NH -N) while the continuous measurements of gas flux showed a durative reduction in N O flux by high Zn input (40mgL ) during the low tide period. Soil inorganic nitrogen concentrations increased at the end of the experiment and even more soil NH -N was measured in the high-Zn-level treatment, indicating an inhibition of ammonia oxidation by Zn input. Quantitative PCR of soil amoA, narG and nirK genes encoding ammonia monooxygenase, nitrate reductase and nitrite reductase, respectively, showed that the microbial abundances involved in these metabolisms were neither affected by wastewater discharge nor Zn contamination.
[Mh] Termos MeSH primário: Óxido Nitroso/análise
Microbiologia do Solo
Solo/química
Águas Residuais
Zinco/análise
[Mh] Termos MeSH secundário: Animais
Archaea/metabolismo
Bactérias/metabolismo
Genes Arqueais
Genes Bacterianos
Gado
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Waste Water); J41CSQ7QDS (Zinc); K50XQU1029 (Nitrous Oxide)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


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[PMID]:27871372
[Au] Autor:Stekhanova TN; Rakitin AL; Mardanov AV; Bezsudnova EY; Popov VO
[Ad] Endereço:A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071, Moscow, Russian Federation. Electronic address: tstekh@yandex.ru.
[Ti] Título:A Novel highly thermostable branched-chain amino acid aminotransferase from the crenarchaeon Vulcanisaeta moutnovskia.
[So] Source:Enzyme Microb Technol;96:127-134, 2017 Jan.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new fold-type IV branched-chain amino acid aminotransferase VMUT0738 from the hyperthermophilic Crenarchaeon Vulcanisaeta moutnovskia was successfully expressed in Escherichia coli. Purified VMUT0738 showed activity toward numerous aliphatic and aromatic l-amino acids and 2-oxo acids at optimal pH 8.0. Distinguishing features of the VMUT0738 compared with typical BCAT are the absence of activity toward acidic substrates, high activity toward basic ones, and low but detectable activity toward the (R)-enantiomer of α-methylbenzylamine (0.0076U/mg) The activity of VMUT0738 increases with a rise in the temperature from 60°C to 90°C. VMUT0738 showed high thermostability (after 24h incubation at 70°C the enzyme lost only 27% of the initial activity) and the resistance to organic solvents. The sequence alignment revealed two motifs (V/I)xLDxR and PFG(K/H)YL characteristic of BCATs from species of the related genera Vulcanisaeta, Pyrobaculum and Thermoproteus that might be responsible for the unique substrate recognition profile of the enzyme.
[Mh] Termos MeSH primário: Proteínas Arqueais/metabolismo
Thermoproteaceae/enzimologia
Transaminases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas Arqueais/genética
Clonagem Molecular
Estabilidade Enzimática
Genes Arqueais
Concentração de Íons de Hidrogênio
Cinética
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
Homologia Estrutural de Proteína
Especificidade por Substrato
Temperatura Ambiente
Thermoproteaceae/genética
Transaminases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Recombinant Proteins); EC 2.6.1.- (Transaminases); EC 2.6.1.42 (branched-chain-amino-acid transaminase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE



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