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Pesquisa : G05.360.340.024.340.364.500 [Categoria DeCS]
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  1 / 21972 MEDLINE  
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[PMID]:29351285
[Au] Autor:Peltier E; Bernard M; Trujillo M; Prodhomme D; Barbe JC; Gibon Y; Marullo P
[Ad] Endereço:Univ. Bordeaux, ISVV, Unité de recherche OEnologie EA 4577, USC 1366 INRA, Bordeaux INP, Villenave d'Ornon, France.
[Ti] Título:Wine yeast phenomics: A standardized fermentation method for assessing quantitative traits of Saccharomyces cerevisiae strains in enological conditions.
[So] Source:PLoS One;13(1):e0190094, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This work describes the set up of a small scale fermentation methodology for measuring quantitative traits of hundreds of samples in an enological context. By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following their weight loss over the time. This dispositive was coupled with robotized enzymatic assays for measuring metabolites of enological interest in natural grape juices. Despite the small volume used, kinetic parameters and fermentation end products measured are similar with those observed in larger scale vats. The vessel used also offers the possibility to assay 32 volatiles compounds using a headspace solid-phase micro-extraction coupled to gas chromatography and mass spectrometry. The vessel shaking applied strongly impacted most of the phenotypes investigated due to oxygen transfer occuring in the first hours of the alcoholic fermentation. The impact of grape must and micro-oxygenation was investigated illustrating some relevant genetic x environmental interactions. By phenotyping a wide panel of commercial wine starters in five grape juices, broad phenotypic correlations between kinetics and metabolic end products were evidentiated. Moreover, a multivariate analysis illustrates that some grape musts are more able than others to discriminate commercial strains since some are less robust to environmental changes.
[Mh] Termos MeSH primário: Fermentação
Locos de Características Quantitativas
Saccharomyces cerevisiae/metabolismo
Vinho
[Mh] Termos MeSH secundário: Genes Fúngicos
Saccharomyces cerevisiae/classificação
Saccharomyces cerevisiae/genética
Especificidade da Espécie
Vitis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190094


  2 / 21972 MEDLINE  
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[PMID]:29304067
[Au] Autor:Abrhámová K; Nemcko F; Libus J; Prevorovský M; Hálová M; Puta F; Folk P
[Ad] Endereço:Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic.
[Ti] Título:Introns provide a platform for intergenic regulatory feedback of RPL22 paralogs in yeast.
[So] Source:PLoS One;13(1):e0190685, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ribosomal protein genes (RPGs) in Saccharomyces cerevisiae are a remarkable regulatory group that may serve as a model for understanding genetic redundancy in evolutionary adaptations. Most RPGs exist as pairs of highly conserved functional paralogs with divergent untranslated regions and introns. We examined the roles of introns in strains with various combinations of intron and gene deletions in RPL22, RPL2, RPL16, RPL37, RPL17, RPS0, and RPS18 paralog pairs. We found that introns inhibited the expression of their genes in the RPL22 pair, with the RPL22B intron conferring a much stronger effect. While the WT RPL22A/RPL22B mRNA ratio was 93/7, the rpl22aΔi/RPL22B and RPL22A/rpl22bΔi ratios were >99/<1 and 60/40, respectively. The intron in RPL2A stimulated the expression of its own gene, but the removal of the other introns had little effect on expression of the corresponding gene pair. Rpl22 protein abundances corresponded to changes in mRNAs. Using splicing reporters containing endogenous intron sequences, we demonstrated that these effects were due to the inhibition of splicing by Rpl22 proteins but not by their RNA-binding mutant versions. Indeed, only WT Rpl22A/Rpl22B proteins (but not the mutants) interacted in a yeast three-hybrid system with an RPL22B intronic region between bp 165 and 236. Transcriptome analysis showed that both the total level of Rpl22 and the A/B ratio were important for maintaining the WT phenotype. The data presented here support the contention that the Rpl22B protein has a paralog-specific role. The RPL22 singleton of Kluyveromyces lactis, which did not undergo whole genome duplication, also responded to Rpl22-mediated inhibition in K. lactis cells. Vice versa, the overproduction of the K. lactis protein reduced the expression of RPL22A/B in S. cerevisiae. The extraribosomal function of of the K. lactis Rpl22 suggests that the loop regulating RPL22 paralogs of S. cerevisiae evolved from autoregulation.
[Mh] Termos MeSH primário: Genes Fúngicos
Íntrons
Kluyveromyces/genética
[Mh] Termos MeSH secundário: Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190685


  3 / 21972 MEDLINE  
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[PMID]:29287097
[Au] Autor:López LF; Muñoz CO; Cáceres DH; Tobón ÁM; Loparev V; Clay O; Chiller T; Litvintseva A; Gade L; González Á; Gómez BL
[Ad] Endereço:Medical and Experimental Mycology Group, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.
[Ti] Título:Standardization and validation of real time PCR assays for the diagnosis of histoplasmosis using three molecular targets in an animal model.
[So] Source:PLoS One;12(12):e0190311, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Histoplasmosis is considered one of the most important endemic and systemic mycoses worldwide. Until now few molecular techniques have been developed for its diagnosis. The aim of this study was to develop and evaluate three real time PCR (qPCR) protocols for different protein-coding genes (100-kDa, H and M antigens) using an animal model. Fresh and formalin-fixed and paraffin-embedded (FFPE) lung tissues from BALB/c mice inoculated i.n. with 2.5x106 Histoplasma capsulatum yeast or PBS were obtained at 1, 2, 3, 4, 8, 12 and 16 weeks post-infection. A collection of DNA from cultures representing different clades of H. capsulatum (30 strains) and other medically relevant pathogens (36 strains of related fungi and Mycobacterium tuberculosis) were used to analyze sensitivity and specificity. Analytical sensitivity and specificity were 100% when DNAs from the different strains were tested. The highest fungal burden occurred at first week post-infection and complete fungal clearance was observed after the third week; similar results were obtained when the presence of H. capsulatum yeast cells was demonstrated in histopathological analysis. In the first week post-infection, all fresh and FFPE lung tissues from H. capsulatum-infected animals were positive for the qPCR protocols tested except for the M antigen protocol, which gave variable results when fresh lung tissue samples were analyzed. In the second week, all qPCR protocols showed variable results for both fresh and FFPE tissues. Samples from the infected mice at the remaining times post-infection and uninfected mice (controls) were negative for all protocols. Good agreement was observed between CFUs, histopathological analysis and qPCR results for the 100-kDa and H antigen protocols. We successfully standardized and validated three qPCR assays for detecting H. capsulatum DNA in fresh and FFPE tissues, and conclude that the 100-kDa and H antigen molecular assays are promising tests for diagnosing this mycosis.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Genes Fúngicos
Histoplasmose/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
DNA Fúngico/genética
DNA Fúngico/isolamento & purificação
Histoplasma/genética
Histoplasmose/genética
Pulmão/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Mycobacterium tuberculosis/genética
Mycobacterium tuberculosis/isolamento & purificação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190311


  4 / 21972 MEDLINE  
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[PMID]:29293626
[Au] Autor:Xiang Q; Li J; Qin P; He M; Yu X; Zhao K; Zhang X; Ma M; Chen Q; Chen X; Zeng X; Gu Y
[Ad] Endereço:College of Resource, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China.
[Ti] Título:Identification and evaluation of reference genes for qRT-PCR studies in Lentinula edodes.
[So] Source:PLoS One;13(1):e0190226, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lentinula edodes (shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. It contains various medically important molecules, such as polysaccharides, terpenoids, sterols, and lipids, were contained in this mushroom. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to analyze the mechanisms underlying the biosynthetic pathways of these substances. qRT-PCR is used for accurate analyses of transcript levels owing to its rapidity, sensitivity, and reliability. However, its accuracy and reliability for the quantification of transcripts rely on the expression stability of the reference genes used for data normalization. To ensure the reliability of gene expression analyses using qRT-PCR in L. edodes molecular biology research, it is necessary to systematically evaluate reference genes. In the current study, ten potential reference genes were selected from L. edodes genomic data and their expression levels were measured by qRT-PCR using various samples. The expression stability of each candidate gene was analyzed by three commonly used software packages: geNorm, NormFinder, and BestKeeper. Base on the results, Rpl4 was the most stable reference gene across all experimental conditions, and Atu was the most stable gene among strains. 18S was found to be the best reference gene for different development stages, and Rpl4 was the most stably expressed gene under various nutrient conditions. The present work will contribute to qRT-PCR studies in L. edodes.
[Mh] Termos MeSH primário: Genes Fúngicos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Cogumelos Shiitake/genética
[Mh] Termos MeSH secundário: Regulação Fúngica da Expressão Gênica
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190226


  5 / 21972 MEDLINE  
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[PMID]:28465214
[Au] Autor:Guo J; Wang Y; Li B; Huang S; Chen Y; Guo X; Xiao D
[Ad] Endereço:Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education College of Biotechnology, Tianjin University of Science Technology, Tianjin, 300457, China.
[Ti] Título:Development of a one-step gene knock-out and knock-in method for metabolic engineering of Aureobasidium pullulans.
[So] Source:J Biotechnol;251:145-150, 2017 Jun 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aureobasidium pullulans is an increasingly attractive host for bio-production of pullulan, heavy oil, polymalic acid, and a large spectrum of extracellular enzymes. To date, genetic manipulation of A. pullulans mainly relies on time-consuming conventional restriction enzyme digestion and ligation methods. In this study, we present a one-step homologous recombination-based method for rapid genetic manipulation in A. pullulans. Overlaps measuring >40bp length and 10µg DNA segments for homologous recombination provided maximum benefits to transformation of A. pullulans. This optimized method was successfully applied to PKSIII gene (encodes polyketide synthase) knock-out and gltP gene (encodes glycolipid transfer protein) knock-in. After disruption of PKSIII gene, secretion of melanin decreased slightly. The melanin purified from disruptant showed lower reducing capacity compared with that of the parent strain, leading to a decrease in exopolysaccharide production. Knock-in of gltP gene resulted in at least 4.68-fold increase in heavy oil production depending on the carbon source used, indicating that gltP can regulate heavy oil synthesis in A. pullulans.
[Mh] Termos MeSH primário: Ascomicetos/genética
Ascomicetos/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Transporte/genética
Fermentação
Proteínas Fúngicas/metabolismo
Técnicas de Introdução de Genes
Técnicas de Inativação de Genes
Genes Fúngicos
Glucose/metabolismo
Melaninas/metabolismo
Engenharia Metabólica
Policetídeo Sintases/genética
Recombinação Genética
Xilose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Fungal Proteins); 0 (Melanins); 0 (lipid transfer protein); 79956-01-7 (Polyketide Synthases); A1TA934AKO (Xylose); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  6 / 21972 MEDLINE  
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[PMID]:28465213
[Au] Autor:Zhao C; Gao Q; Chen J; Wei L; Imanaka T; Hua Q
[Ad] Endereço:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China.
[Ti] Título:Metabolomic changes and metabolic responses to expression of heterologous biosynthetic genes for lycopene production in Yarrowia lipolytica.
[So] Source:J Biotechnol;251:174-185, 2017 Jun 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The metabolomic studies were conducted for both the engineered lycopene-producing Yarrowia lipolytica strain and the control strain. The changes in metabolome profiling and main pathway abundance of Y. lipolytica with the introduction of heterologous crt genes were investigated by multiple analytical and statistical methods More importantly, the metabolome data were transformed into pathway abundances visualized by a pathway relationship network using Cytoscape software. This network provided a simple way to present the statistical results of pathway abundance and interpret the effects of the crt genes in a metabolic view, and the results highlighted the up-regulation of eight pathways and the down-regulation of three pathways in the lycopene fast production phase. The comprehensive analysis suggested that the synthesis of lycopene has remarkable effects on fructose and mannose metabolism, citrate cycle, glyoxylate and dicarboxylate metabolism, glycerophospholipid metabolism as well as the biosynthesis and metabolism of some amino acids including valine, leucine and isoleucine in Y. lipolytica.
[Mh] Termos MeSH primário: Carotenoides/biossíntese
Yarrowia/genética
Yarrowia/metabolismo
[Mh] Termos MeSH secundário: Carotenoides/genética
Genes Fúngicos
Metaboloma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
36-88-4 (Carotenoids); SB0N2N0WV6 (lycopene)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  7 / 21972 MEDLINE  
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[PMID]:29284018
[Au] Autor:Wang H; Li X; Li X; Li X; Wang J; Zhang H
[Ad] Endereço:Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
[Ti] Título:Changes of microbial population and N-cycling function genes with depth in three Chinese paddy soils.
[So] Source:PLoS One;12(12):e0189506, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microbial communities play critical roles in soil nitrogen (N) cycle; however, we have limited understanding of the distribution of N-cycling microbial groups in deeper soil horizons. In this study, we used quantitative PCR to characterize the changes of microbial populations (16S rRNA and 18S rRNA) and five key N-cycling gene abundances involved in N fixation (nifH), ammonia oxidation (amoA) by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA), and nitrite reduction (nirS and nirK) along profiles (0-100 cm depth) of different paddy soils from three regions (Hailun, Changshu, Yingtan) across China from north to south. We found that most microbial and N-cycling functional genes significantly decreased with soil depth; however, AOA were enriched in deeper soil layers (20-40 cm). The abundances of microbial and N-cycling functional genes generally decreased by one to two orders of magnitude in the deeper horizons relative to topsoils. The AOA gene abundance was higher than that of AOB in the paddy soil profile, and the nirS and nirK abundances were dominant in topsoil and deeper soil, respectively. All N functional genes except AOA were more abundant in Changshu than Hailun and Yingtan. High abundances and low vertical changes of N-cycling genes in Changshu suggest more dynamic N-transformations in this region. Correlation analysis showed that soil properties and climate parameters had a significant relationship with N-cycling gene abundances. Moreover, the abundance of different N-cycling genes was affected by different environmental parameters, which should be studied further to explore their roles in N cycling for sustainable agriculture and environmental management.
[Mh] Termos MeSH primário: Agricultura
Microbiologia do Solo
[Mh] Termos MeSH secundário: Bactérias/genética
Bactérias/isolamento & purificação
Bactérias/metabolismo
China
Fungos/genética
Fungos/isolamento & purificação
Fungos/metabolismo
Genes Bacterianos
Genes Fúngicos
Nitrogênio/metabolismo
RNA Ribossômico 16S/genética
RNA Ribossômico 18S/genética
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 18S); N762921K75 (Nitrogen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189506


  8 / 21972 MEDLINE  
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[PMID]:27775185
[Au] Autor:Oakley CE; Ahuja M; Sun WW; Entwistle R; Akashi T; Yaegashi J; Guo CJ; Cerqueira GC; Russo Wortman J; Wang CC; Chiang YM; Oakley BR
[Ad] Endereço:Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas, 66045, USA.
[Ti] Título:Discovery of McrA, a master regulator of Aspergillus secondary metabolism.
[So] Source:Mol Microbiol;103(2):347-365, 2017 Jan.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fungal secondary metabolites (SMs) are extremely important in medicine and agriculture, but regulation of their biosynthesis is incompletely understood. We have developed a genetic screen in Aspergillus nidulans for negative regulators of fungal SM gene clusters and we have used this screen to isolate mutations that upregulate transcription of the non-ribosomal peptide synthetase gene required for nidulanin A biosynthesis. Several of these mutations are allelic and we have identified the mutant gene by genome sequencing. The gene, which we designate mcrA, is conserved but uncharacterized, and it encodes a putative transcription factor. Metabolite profiles of mcrA deletant, mcrA overexpressing, and parental strains reveal that mcrA regulates at least ten SM gene clusters. Deletion of mcrA stimulates SM production even in strains carrying a deletion of the SM regulator laeA, and deletion of mcrA homologs in Aspergillus terreus and Penicillum canescens alters the secondary metabolite profile of these organisms. Deleting mcrA in a genetic dereplication strain has allowed us to discover two novel compounds as well as an antibiotic not known to be produced by A. nidulans. Deletion of mcrA upregulates transcription of hundreds of genes including many that are involved in secondary metabolism, while downregulating a smaller number of genes.
[Mh] Termos MeSH primário: Aspergillus nidulans/genética
Aspergillus nidulans/metabolismo
Peptídeo Sintases/genética
Peptídeo Sintases/metabolismo
[Mh] Termos MeSH secundário: Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Genes Fúngicos
Família Multigênica
Mutação
Metabolismo Secundário
Deleção de Sequência
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Transcription Factors); EC 6.3.2.- (Peptide Synthases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13562


  9 / 21972 MEDLINE  
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[PMID]:28987344
[Au] Autor:Omidi K; Jessulat M; Hooshyar M; Burnside D; Schoenrock A; Kazmirchuk T; Hajikarimlou M; Daniel M; Moteshareie H; Bhojoo U; Sanders M; Ramotar D; Dehne F; Samanfar B; Babu M; Golshani A
[Ad] Endereço:Department of Biology, Carleton University, Ottawa, Ontario, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
[Ti] Título:Uncharacterized ORF HUR1 influences the efficiency of non-homologous end-joining repair in Saccharomyces cerevisiae.
[So] Source:Gene;639:128-136, 2018 Jan 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Non-Homologous End Joining (NHEJ) is a highly conserved pathway that repairs Double-Strand Breaks (DSBs) within DNA. Here we show that the deletion of yeast uncharacterized ORF HUR1, Hydroxyurea Resistance1 affects the efficiency of NHEJ. Our findings are supported by Protein-Protein Interaction (PPI), genetic interaction and drug sensitivity analyses. To assess the activity of HUR1 in DSB repair, we deleted its non-overlapping region with PMR1, referred to as HUR1-A. We observed that similar to deletion of TPK1 and NEJ1, and unlike YKU70 (important for NHEJ of DNA with overhang and not blunt end), deletion of HUR1-A reduced the efficiency of NHEJ in both overhang and blunt end plasmid repair assays. Similarly, a chromosomal repair assay showed a reduction for repair efficiency when HUR1-A was deleted. In agreement with a functional connection for Hur1p with Tpk1p and NEJ1p, double mutant strains Δhur1-A/Δtpk1, and Δhur1-A/Δnej1 showed the same reduction in the efficiency of plasmid repair, compared to both single deletion strains. Also, using a Homologous Recombination (HR) specific plasmid-based DSB repair assay we observed that deletion of HUR1-A influenced the efficiency of HR repair, suggesting that HUR1 might also play additional roles in other DNA repair pathways.
[Mh] Termos MeSH primário: Reparo do DNA por Junção de Extremidades
Fases de Leitura Aberta
Proteínas de Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Genes Fúngicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  10 / 21972 MEDLINE  
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[PMID]:29186149
[Au] Autor:Hrabáková L; Grum-Grzhimaylo AA; Koloniuk I; Debets AJM; Sarkisova T; Petrzik K
[Ad] Endereço:Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
[Ti] Título:The alkalophilic fungus Sodiomyces alkalinus hosts beta- and gammapartitiviruses together with a new fusarivirus.
[So] Source:PLoS One;12(11):e0187799, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mixed infection by three dsRNA viruses, a novel betapartitivirus, a gammapartitivirus, and a novel fusarivirus, has been identified in four isolates of the obligate alkalophilic fungus Sodiomyces alkalinus. The first, Sodiomyces alkalinus partitivirus 1 (SaPV1), is placed within the genus Betapartitivirus and is related to Ustilaginoidea virens partitivirus 2. The taxonomic position of the second virus is less clear as it shares high (85%) amino acid sequence identity but significantly low (77%) nucleotide sequence identity of the capsid protein with Colletotrichum truncatum partitivirus 1. The third, the novel Sodiomyces alkalinus fusarivirus 1 (SaFV1), is related to Fusarium poae fusarivirus 1. All the viruses show efficient vertical transmission through asexual and sexual spores. These novel coexisting viruses do not evoke apparent phenotypic alteration to their fungal host. This is the first description of a viral infection in an alkalophilic fungus.
[Mh] Termos MeSH primário: Ascomicetos/virologia
Interações Hospedeiro-Patógeno
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: Ascomicetos/classificação
Ascomicetos/genética
Genes Fúngicos
Sequenciamento de Nucleotídeos em Larga Escala
Filogenia
Vírus de RNA/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187799



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