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  1 / 1968 MEDLINE  
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[PMID]:28464889
[Au] Autor:de Azevedo SSD; Caetano DG; Côrtes FH; Teixeira SLM; Dos Santos Silva K; Hoagland B; Grinsztejn B; Veloso VG; Morgado MG; Bello G
[Ad] Endereço:Laboratório de AIDS & Imunologia Molecular, Instituto Oswaldo Cruz - FIOCRUZ, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil.
[Ti] Título:Highly divergent patterns of genetic diversity and evolution in proviral quasispecies from HIV controllers.
[So] Source:Retrovirology;14(1):29, 2017 May 02.
[Is] ISSN:1742-4690
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ongoing intra-host HIV-1 evolution has been shown in individuals that naturally suppress the viremia to low levels (HIV controllers) by the analysis of the RNA in plasma compartment. Detection of evolution at the DNA proviral compartment in HIV controllers, however, has been more challenging and the precise correlation between the systemic viral suppression level and rate of reservoir's reseeding in those individuals is not fully understood. In this sense, we examined the proviral DNA quasispecies by single genome amplification of the env gene in a cohort of 23 HIV controllers from Brazil, divided in three groups, according to the level of systemic viral suppression: (1) elite controllers with persistent undetectable viral load (PEC, n = 6); (2) elite controllers with occasional episodes of transient (51-400 copies/mL) viremia (EEC, n = 7); and (3) viremic controllers with persistent low-level (80-2000 copies/mL) viremia (VC, n = 10). RESULTS: The HIV-1 diversity of the PBMC-associated proviral quasispecies in EC was significantly (P < 0.01) lower than in VC, but not significantly different between PEC and EEC groups. We detected a considerable variation in the average pairwise nucleotide distance and proportion of unique sequences in the HIV-1 proviral quasispecies of PEC and EEC. Some PEC and EEC displayed highly homogenous proviral populations with large clusters of identical sequences, while others exhibited relatively diverse proviral populations with a high proportion of unique sequences comparable to VC subjects. The long-term (10-15 years) follow-up of the HIV-1 proviral populations revealed a complete evolutionary stasis in one PEC and measurable divergence rates in one EEC [3.1 (1.2-5.6) × 10 substitutions/site/year and one VC [2.9 (0.7-5.1) × 10 substitutions/site/year]. CONCLUSIONS: There is no simple relationship between systemic viral suppression and intra-host proviral diversity or rate of reservoir's reseeding in chronically infected HIV controllers. Our results demonstrate that very divergent patterns of intra-host viral diversity and divergence could be detected in the setting of natural suppression of HIV-1 replication and that ongoing evolution and reseeding of the PBMC proviral reservoir occurs in some elite controllers.
[Mh] Termos MeSH primário: Evolução Molecular
Variação Genética
Infecções por HIV/virologia
HIV-1/genética
Provírus/genética
Quase-Espécies
Carga Viral
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Brasil
Contagem de Linfócito CD4
Estudos de Coortes
Feminino
Genes env
Genoma Viral
Infecções por HIV/imunologia
HIV-1/fisiologia
Seres Humanos
Masculino
Meia-Idade
RNA Viral/sangue
Viremia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12977-017-0354-5


  2 / 1968 MEDLINE  
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[PMID]:29224130
[Au] Autor:Wang M; Wang Y; Baloch AR; Pan Y; Xu F; Tian L; Zeng Q
[Ad] Endereço:College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
[Ti] Título:Molecular epidemiology and characterization of bovine leukemia virus in domestic yaks (Bos grunniens) on the Qinghai-Tibet Plateau, China.
[So] Source:Arch Virol;163(3):659-670, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV infections in domestic yaks and determine the genetic variability of BLV circulating in a region of the Qinghai Tibet Plateau, China. Blood samples were collected from 798 yaks, which were from different farms from Gansu, Qinghai and Sichuan provinces surrounding the Qinghai-Tibet Plateau. Nested PCR targeting BLV long terminal repeats was used to detect the BLV provirus. The highest prevalence of BLV infection was in Gansu province, where it was 18.93% (39/206) in white yaks from Tianzhu City and 19.14% (31/162) in black yaks from Gannan City. In Qinghai and Sichuan provinces, the prevalence of BLV in black yaks was 14.83% (35/236) and 14.94% (29/194), respectively. The prevalence of BLV was not significantly different in yaks up to one year old than in older animals. Phylogenetic analysis was performed using 16 different env-gp51 (497-bp) gene sequences from the three provinces and 71 known BLV strains, which revealed that in both Gansu and Qinghai provinces, genotypes 6 and 10 of the BLV strains were at high levels, whereas only genotype 10 was prevalent in Sichuan Province. Phylogenetic analysis and sequence comparisons revealed 95.7-99.8% sequence identity among the full-length env genes of 16 strains, nearly full-length genome sequences of six BLV strains, and those of the known genotypes 6 and 10 of BLV. This study provides comprehensive information is regarding the widespread infection of domestic yaks with BLV on the Qinghai-Tibet Plateau of China, and shows that at least two BLV genotypes (genotypes 6 and 10) are circulating in this population.
[Mh] Termos MeSH primário: Leucose Enzoótica Bovina/epidemiologia
Genes env
Genótipo
Vírus da Leucemia Bovina/classificação
Vírus da Leucemia Bovina/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Bovinos
Leucose Enzoótica Bovina/transmissão
Leucose Enzoótica Bovina/virologia
Expressão Gênica
Vírus da Leucemia Bovina/isolamento & purificação
Epidemiologia Molecular
Prevalência
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Sequências Repetidas Terminais
Tibet/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3658-9


  3 / 1968 MEDLINE  
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[PMID]:28768859
[Au] Autor:Gonzalez-Perez MP; Peters PJ; O'Connell O; Silva N; Harbison C; Cummings Macri S; Kaliyaperumal S; Luzuriaga K; Clapham PR
[Ad] Endereço:Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA MariaPaz.Gonzalez-Perez@umassmed.edu.
[Ti] Título:Identification of Emerging Macrophage-Tropic HIV-1 R5 Variants in Brain Tissue of AIDS Patients without Severe Neurological Complications.
[So] Source:J Virol;91(20), 2017 Oct 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Untreated HIV-positive (HIV-1 ) individuals frequently suffer from HIV-associated neurocognitive disorders (HAND), with about 30% of AIDS patients suffering severe HIV-associated dementias (HADs). Antiretroviral therapy has greatly reduced the incidence of HAND and HAD. However, there is a continuing problem of milder neurocognitive impairments in treated HIV patients that may be increasing with long-term therapy. In the present study, we investigated whether envelope ( ) genes could be amplified from proviral DNA or RNA derived from brain tissue of 12 individuals with normal neurology or minor neurological conditions (N/MC individuals). The tropism and characteristics of the brain-derived Envs were then investigated and compared to those of Envs derived from immune tissue. We showed that (i) macrophage-tropic R5 Envs could be detected in the brain tissue of 4/12 N/MC individuals, (ii) macrophage-tropic Envs in brain tissue formed compartmentalized clusters distinct from non-macrophage-tropic (non-mac-tropic) Envs recovered from the spleen or brain, (iii) the evidence was consistent with active viral expression by macrophage-tropic variants in the brain tissue of some individuals, and (iv) Envs from immune tissue of the N/MC individuals were nearly all tightly non-mac-tropic, contrasting with previous data for neuro-AIDS patients where immune tissue Envs mediated a range of macrophage infectivities, from background levels to modest infection, with a small number of Envs from some patients mediating high macrophage infection levels. In summary, the data presented here show that compartmentalized and active macrophage-tropic HIV-1 variants are present in the brain tissue of individuals before neurological disease becomes overt or serious. The detection of highly compartmentalized macrophage-tropic R5 Envs in the brain tissue of HIV patients without serious neurological disease is consistent with their emergence from a viral population already established there, perhaps from early disease. The detection of active macrophage-tropic virus expression, and probably replication, indicates that antiretroviral drugs with optimal penetration through the blood-brain barrier should be considered even for patients without neurological disease (neuro-disease). Finally, our data are consistent with the brain forming a sanctuary site for latent virus and low-level viral replication in the absence of neuro-disease.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida/virologia
Encéfalo/virologia
HIV-1/isolamento & purificação
HIV-1/fisiologia
Macrófagos/virologia
Tropismo Viral
[Mh] Termos MeSH secundário: Síndrome de Imunodeficiência Adquirida/complicações
Síndrome de Imunodeficiência Adquirida/tratamento farmacológico
Barreira Hematoencefálica
Genes env
HIV-1/genética
Seres Humanos
Vírion/genética
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE


  4 / 1968 MEDLINE  
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[PMID]:28379444
[Au] Autor:Lamichhane R; Hammond JA; Pauszek RF; Anderson RM; Pedron I; van der Schans E; Williamson JR; Millar DP
[Ad] Endereço:Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
[Ti] Título:A DEAD-box protein acts through RNA to promote HIV-1 Rev-RRE assembly.
[So] Source:Nucleic Acids Res;45(8):4632-4641, 2017 May 05.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The HIV-1 Rev protein activates nuclear export of unspliced and partially spliced viral RNA transcripts, which encode the viral genome and the genes encoding viral structural proteins, by binding to and oligomerizing on the Rev Response Element (RRE). The human DEAD-box protein 1 (DDX1) enhances the RNA export activity of Rev through an unknown mechanism. Using a single-molecule assembly assay and various DDX1 mutants, we show that DDX1 acts through the RRE RNA to specifically accelerate the nucleation step of the Rev-RRE assembly process. Single-molecule Förster resonance energy transfer (smFRET) experiments using donor-labeled Rev and acceptor-labeled DDX1 show that both proteins can associate with a single RRE molecule. However, simultaneous interaction is only observed in a subset of binding events and does not explain the extent to which DDX1 promotes the nucleation step of Rev-RRE assembly. Together, these results are consistent with a model wherein DDX1 acts as an RNA chaperone, remodeling the RRE into a conformation that is pre-organized to bind the first Rev monomer, thereby promoting the overall Rev-RRE assembly process.
[Mh] Termos MeSH primário: RNA Helicases DEAD-box/genética
Genes env
HIV-1/genética
RNA Mensageiro/genética
RNA Viral/genética
Montagem de Vírus/genética
[Mh] Termos MeSH secundário: Sítios de Ligação
Transporte Biológico
Carbocianinas/química
RNA Helicases DEAD-box/metabolismo
Transferência Ressonante de Energia de Fluorescência
Corantes Fluorescentes/química
Expressão Gênica
HIV-1/crescimento & desenvolvimento
HIV-1/metabolismo
Interações Hospedeiro-Patógeno
Seres Humanos
Conformação de Ácido Nucleico
Ligação Proteica
RNA Mensageiro/química
RNA Mensageiro/metabolismo
RNA Viral/química
RNA Viral/metabolismo
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Rodaminas/química
Imagem Individual de Molécula
Coloração e Rotulagem
Ácidos Sulfônicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alexa Fluor 555); 0 (Alexa Fluor 647); 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (Recombinant Fusion Proteins); 0 (Rhodamines); 0 (Sulfonic Acids); EC 3.6.1.- (DDX1 protein, human); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx206


  5 / 1968 MEDLINE  
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[PMID]:28331116
[Au] Autor:Marawan MA; Mekata H; Hayashi T; Sekiguchi S; Kirino Y; Horii Y; Moustafa AM; Arnaout FK; Galila ESM; Norimine J
[Ad] Endereço:Laboratory of Animal Infectious Disease and Prevention, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan.
[Ti] Título:Phylogenetic analysis of env gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan.
[So] Source:J Vet Med Sci;79(5):912-916, 2017 May 18.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To understand how the latest dominant bovine leukemia virus (BLV) strains were introduced and spread in the Miyazaki prefecture, we collected blood samples from 3 geographic areas (north, central and south) and carried out sequence analysis of the BLV env gene. Two genotypes, genotype I, and III, were identified and the majority of the strains belonged to genotype I (71/74). To clarify a route of BLV introduction, we divided the strains into 20 subgenotypes based on their nucleotide sequences and performed phylogenetic analysis. Our study indicated that common BLV strains were comparatively evenly distributed even in the area, where the farmers have not introduced cattle from other areas and the cattle have limited exposure to BLV infection in grazing fields.
[Mh] Termos MeSH primário: Leucose Enzoótica Bovina/virologia
Genes env
Vírus da Leucemia Bovina/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
DNA Viral
Japão
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.17-0055


  6 / 1968 MEDLINE  
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[PMID]:28213870
[Au] Autor:Pluta A; Rola-Luszczak M; Kubis P; Balov S; Moskalik R; Choudhury B; Kuzmak J
[Ad] Endereço:OIE Reference Laboratory for EBL, Department of Biochemistry, National Veterinary Research Institute, Pulawy, Poland. aneta.pluta@piwet.pulawy.pl.
[Ti] Título:Molecular characterization of bovine leukemia virus from Moldovan dairy cattle.
[So] Source:Arch Virol;162(6):1563-1576, 2017 Jun.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cattle from three farms in three regions of Moldova were screened by ELISA and nested PCR. Of these cattle, 14 which were PCR positive, and these were molecularly characterized based on the nucleotide sequence of the env gene and the deduced amino acid sequence of the encoded gp51 protein. Our results demonstrated a low level of genetic variability (0-2.9%) among BLV field strains from Moldova, in contrast to that observed for other retroviruses, including human immunodeficiency virus (HIV) (20-38%) Mason IL (Trudy vologod moloch Inst 146-164, 1970) and equine infectious anemia virus (EIAV) (~40%) Willems L et al (AIDS Res Hum Retroviruses 16(16):1787-1795, 2000), where the envelope gene exhibits high levels of variation Polat M et al (Retrovirology 13(1):4, 2016). Sequence comparisons and phylogenetic analysis revealed that BLV genotype 7 (G7) is predominant in Moldova and that the BLV population in Moldovan cattle is a mixture of at least three new sub-genotypes: G7D, G7E and G4C. Neutrality tests revealed that negative selection was the major force operating upon the 51-kDa BLV envelope surface glycoprotein subunit gp51, although one positively selected site within conformational epitope G was detected in the N-terminal part of gp51. Furthermore, two functional domains, linear epitope B and the zinc-binding domain, were found to have an elevated ratio of nonsynonymous to synonymous codon differences. Together, these data suggest that the evolutionary constraints on epitopes G and B and the zinc-binding domains of gp51 differ from those on the other domains, with a tendency towards formation of homogenous genetic groups, which is a common concept of global BLV diversification during virus transmission that may be associated with genetic drift.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Indústria de Laticínios
Leucose Enzoótica Bovina/virologia
Variação Genética
Vírus da Leucemia Bovina/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Leucose Enzoótica Bovina/epidemiologia
Genes env/genética
Genótipo
Seres Humanos
Vírus da Leucemia Bovina/classificação
Vírus da Leucemia Bovina/isolamento & purificação
Moldávia/epidemiologia
Filogenia
Reação em Cadeia da Polimerase
Alinhamento de Sequência
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Envelope Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3241-4


  7 / 1968 MEDLINE  
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[PMID]:28100385
[Au] Autor:Liu Y; Jiang LY; Luo L; Cao YM; Jing QL; Yang ZC
[Ad] Endereço:Disinfection Department, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
[Ti] Título:[Phylogenetic analysis of envelope gene of dengue virus serotype 2 in Guangzhou, 2001-2015].
[So] Source:Zhonghua Liu Xing Bing Xue Za Zhi;38(1):90-95, 2017 Jan 10.
[Is] ISSN:0254-6450
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the molecular characteristics of dengue virus serotype 2 (DENV2) in Guangzhou during 2001-2015, and analyze the E gene of the strains isolated, the phylogenetic tree and molecular clock were constructed to know about the evolution of the strains. The serum samples of the patients were detected by real time PCR, and positive samples were used to isolate dengue virus by using C6/36 cells. The E gene of the isolated strains were sequenced. The phylogenetic tree was constructed by using software Mega 4.0, and the molecular clock was drawn by using software BEASTv1.8.2. Twenty-six dengue virus strains were isolated between 2001 and 2015. They were all clustered into 2 genotypes, i.e. cosmopolitan genotype and Asian genotype â… . The strains isolated in Guangzhou shared high homology with Southeast Asian strains. The cosmopolitan genotype was divided into 2 sub-genotype at about 46 and 35 years ago. The substitution rate of dengue virus serotype 2 in Guangzhou was 7.1 × 10(-4) per year per site. There were close relationship between the Guangzhou strains and Southeast Asian strains. Guangzhou was at high risk of imported dengue fever, outbreak of dengue hemorrhagic fever and dengue shock syndrome. There might be two ways of introduction of cosmopolitan genotype. The substitution rate of the strains in Guangzhou was similar to that in the neighbor countries.
[Mh] Termos MeSH primário: Vírus da Dengue/genética
Dengue/epidemiologia
Genes env
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Sequência de Bases
China/epidemiologia
Dengue/virologia
Vírus da Dengue/isolamento & purificação
Surtos de Doenças
Evolução Molecular
Genótipo
Seres Humanos
Epidemiologia Molecular
Filogenia
Reação em Cadeia da Polimerase em Tempo Real
Sorogrupo
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Envelope Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0254-6450.2017.01.018


  8 / 1968 MEDLINE  
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[PMID]:27903772
[Au] Autor:Pocock GM; Zimdars LL; Yuan M; Eliceiri KW; Ahlquist P; Sherer NM
[Ad] Endereço:McArdle Laboratory for Cancer Research and Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI 53706.
[Ti] Título:Diverse activities of viral cis-acting RNA regulatory elements revealed using multicolor, long-term, single-cell imaging.
[So] Source:Mol Biol Cell;28(3):476-487, 2017 Feb 01.
[Is] ISSN:1939-4586
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cis-acting RNA structural elements govern crucial aspects of viral gene expression. How these structures and other posttranscriptional signals affect RNA trafficking and translation in the context of single cells is poorly understood. Herein we describe a multicolor, long-term (>24 h) imaging strategy for measuring integrated aspects of viral RNA regulatory control in individual cells. We apply this strategy to demonstrate differential mRNA trafficking behaviors governed by RNA elements derived from three retroviruses (HIV-1, murine leukemia virus, and Mason-Pfizer monkey virus), two hepadnaviruses (hepatitis B virus and woodchuck hepatitis virus), and an intron-retaining transcript encoded by the cellular NXF1 gene. Striking behaviors include "burst" RNA nuclear export dynamics regulated by HIV-1's Rev response element and the viral Rev protein; transient aggregations of RNAs into discrete foci at or near the nuclear membrane triggered by multiple elements; and a novel, pulsiform RNA export activity regulated by the hepadnaviral posttranscriptional regulatory element. We incorporate single-cell tracking and a data-mining algorithm into our approach to obtain RNA element-specific, high-resolution gene expression signatures. Together these imaging assays constitute a tractable, systems-based platform for studying otherwise difficult to access spatiotemporal features of viral and cellular gene regulation.
[Mh] Termos MeSH primário: Imagem Molecular/métodos
Análise de Célula Única/métodos
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular/fisiologia
Núcleo Celular/metabolismo
Regulação Viral da Expressão Gênica
Produtos do Gene rev/metabolismo
Genes env/fisiologia
HIV-1
Vírus dos Macacos de Mason-Pfizer
Processamento Pós-Transcricional do RNA/fisiologia
RNA Mensageiro/metabolismo
RNA Viral
Sequências Reguladoras de Ácido Nucleico/genética
Sequências Reguladoras de Ácido Nucleico/fisiologia
Sequências Reguladoras de Ácido Ribonucleico/genética
Sequências Reguladoras de Ácido Ribonucleico/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gene Products, rev); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (Regulatory Sequences, Ribonucleic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1091/mbc.E16-08-0612


  9 / 1968 MEDLINE  
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[PMID]:27906032
[Au] Autor:Krebs KC; Tian M; Asmal M; Ling B; Nelson K; Henry K; Gibson R; Li Y; Han W; Shattock RJ; Veazey RS; Letvin N; Arts EJ; Gao Y
[Ad] Endereço:Division of Infectious Diseases, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106 USA.
[Ti] Título:Infection of rhesus macaques with a pool of simian immunodeficiency virus with the envelope genes from acute HIV-1 infections.
[So] Source:AIDS Res Ther;13(1):41, 2016 Nov 25.
[Is] ISSN:1742-6405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: New simian-human immunodeficiency chimeric viruses with an HIV-1 env (SHIVenv) are critical for studies on HIV pathogenesis, vaccine development, and microbicide testing. Macaques are typically exposed to single CCR5-using SHIVenv which in most instances does not reflect the conditions during acute/early HIV infection (AHI) in humans. Instead of individual and serial testing new SHIV constructs, a pool of SHIVenv_B derived from 16 acute HIV-1 infections were constructed using a novel yeast-based SHIV cloning approach and then used to infect macaques. RESULTS: Even though none of the 16 SHIVenvs contained the recently reported mutations in env genes that could significantly enhance their binding affinity to RhCD4, one SHIVenv (i.e. SHIVenv_B3-PRB926) established infection in macaques exposed to this pool. AHI SHIVenv_B viruses as well as their HIVenv_B counterparts were analyzed for viral protein content, function, and fitness to identify possible difference between SHIVenv_B3-PRB926 and the other 15 SHIVenvs in the pool. All of the constructs produced SHIV or HIV chimeric with wild type levels of capsid (p27 and p24) content, reverse transcriptase (RT) activity, and expressed envelope glycoproteins that could bind to cell receptors CD4/CCR5 and mediate virus entry. HIV-1env_B chimeric viruses were propagated in susceptible cell lines but the 16 SHIVenv_B variants showed only limited replication in macaque peripheral blood mononuclear cells (PBMCs) and 174×CEM.CCR5 cell line. AHI chimeric viruses including HIVenv_B3 showed only minor variations in cell entry efficiency and kinetics as well as replicative fitness in human PBMCs. Reduced number of N-link glycosylation sites and slightly greater CCR5 affinity/avidity was the only distinguishing feature of env_B3 versus other AHI env's in the pool, a feature also observed in the HIV establishing new infections in humans. CONCLUSION: Despite the inability to propagate in primary cells and cell lines, a pool of 16 SHIVenv viruses could establish infection but only one virus, SHIVenv_B3 was isolated in the macaque and then shown to repeatedly infected macaques. This SHIVenv_B3 virus did not show any distinct phenotypic property from the other 15 SHIVenv viruses but did have the fewest N-linked glycosylation sites.
[Mh] Termos MeSH primário: Infecções por HIV/genética
HIV-1/genética
Macaca mulatta/virologia
Síndrome de Imunodeficiência Adquirida dos Símios/genética
Síndrome de Imunodeficiência Adquirida dos Símios/virologia
Vírus da Imunodeficiência Símia/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Genes env
Glicosilação
Células HEK293
Infecções por HIV/virologia
Seres Humanos
Mutação
Vírus da Imunodeficiência Símia/patogenicidade
Replicação Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1186/s12981-016-0125-8


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[PMID]:27826543
[Au] Autor:Feng M; Tan Y; Dai M; Li Y; Xie T; Li H; Shi M; Zhang X
[Ad] Endereço:Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural UniversityGuangzhou, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of AgricultureGua
[Ti] Título:Endogenous Retrovirus ev21 Dose Not Recombine with ALV-J and Induces the Expression of ISGs in the Host.
[So] Source:Front Cell Infect Microbiol;6:140, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression. Endogenous viruses integrate into host genomes and can recombine with exogenous avian leukosis virus (ALV). In this study, we analyzed the interaction of endogenous retrovirus 21 ( ) with the ALV-J in late-feathering Chinese yellow chicken. Two ALV-J strains M180 and K243 were isolated from late-feathering and fast-feathering Chinese yellow chicken flocks, respectively. The gene of the two strains showed 94.2-94.8% nucleotide identity with reference ALV-J strains. Compared with the gene and the LTR of and M180, the nucleotide identity of LTR was 69.7% and gene was 58.4%, respectively, especially the amino acid identity of gene as low as 14.2%. Phylogenetic analysis of the nucleotide sequence of the env gene and the 3'LTR showed that M180 was closely related to ALV-J, and was located in a distinct group with in the phylogenetic tree. Using co-immunoprecipitation (co-IP), we next demonstrate that the envelope protein of does not interact with the M180 envelope protein. We further show that the envelope protein of cannot activate ALV-J LTR promoter activity using luciferase-reporter assays. qPCR and western blot analysis revealed that envelope protein of endogenous can facilitate the expression of at 6h post ALV-J infection (hpi) and facilitate the expression of and at 24 hpi. However, the expression of the gene of M180 strain was not significantly at 6 and 24 hpi. We conclude that there is no evidence of recombination between endogenous retrovirus ev21 and ALV-J strain M180 in late-feathering Chinese yellow chicken, and envelope protein of ev21 can affect the expression of host ISGs, but appears not to influence the replication of ALV-J strain M180. This is the first report of interaction among the endogenous retrovirus ev21, ALV-J and the late-feathering chicken.
[Mh] Termos MeSH primário: Vírus da Leucose Aviária/genética
Leucose Aviária/virologia
Retrovirus Endógenos/genética
Doenças das Aves Domésticas/genética
Viroses/genética
Viroses/veterinária
[Mh] Termos MeSH secundário: Animais
Vírus da Leucose Aviária/isolamento & purificação
Sequência de Bases
Linhagem Celular
Galinhas/genética
Galinhas/virologia
DNA Viral
Plumas/virologia
Regulação Viral da Expressão Gênica
Genes env
Imunoprecipitação
Filogenia
Doenças das Aves Domésticas/virologia
RNA Viral/genética
Análise de Sequência
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (RNA, Viral); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE



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