Base de dados : MEDLINE
Pesquisa : G05.360.340.024.340.375.500.791.330 [Categoria DeCS]
Referências encontradas : 4402 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 441 ir para página                         

  1 / 4402 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29184209
[Au] Autor:Rodriguez E; Sakurai K; Xu J; Chen Y; Toda K; Zhao S; Han BX; Ryu D; Yin H; Liedtke W; Wang F
[Ad] Endereço:Department of Neurobiology, Duke University Medical Center, Durham, NC, USA.
[Ti] Título:A craniofacial-specific monosynaptic circuit enables heightened affective pain.
[So] Source:Nat Neurosci;20(12):1734-1743, 2017 Dec.
[Is] ISSN:1546-1726
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Humans often rank craniofacial pain as more severe than body pain. Evidence suggests that a stimulus of the same intensity induces stronger pain in the face than in the body. However, the underlying neural circuitry for the differential processing of facial versus bodily pain remains unknown. Interestingly, the lateral parabrachial nucleus (PB ), a critical node in the affective pain circuit, is activated more strongly by noxious stimulation of the face than of the hindpaw. Using a novel activity-dependent technology called CANE developed in our laboratory, we identified and selectively labeled noxious-stimulus-activated PB neurons and performed comprehensive anatomical input-output mapping. Surprisingly, we uncovered a hitherto uncharacterized monosynaptic connection between cranial sensory neurons and the PB -nociceptive neurons. Optogenetic activation of this monosynaptic craniofacial-to-PB projection induced robust escape and avoidance behaviors and stress calls, whereas optogenetic silencing specifically reduced facial nociception. The monosynaptic circuit revealed here provides a neural substrate for heightened craniofacial affective pain.
[Mh] Termos MeSH primário: Dor Facial/fisiopatologia
Dor Facial/psicologia
Nociceptores
Sinapses
[Mh] Termos MeSH secundário: Afeto
Vias Aferentes/fisiopatologia
Animais
Comportamento Animal
Condicionamento Operante
Feminino
Genes fos/genética
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Optogenética
Estimulação Física
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1038/s41593-017-0012-1


  2 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29240733
[Au] Autor:Zhang Q; He Q; Wang J; Fu C; Hu H
[Ad] Endereço:Interdisciplinary Institute of Neuroscience and Technology, Qiushi Academy for Advanced Studies, Zhejiang University, Hangzhou, China.
[Ti] Título:Use of TAI-FISH to visualize neural ensembles activated by multiple stimuli.
[So] Source:Nat Protoc;13(1):118-133, 2018 Jan.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Researchers in behavioral neuroscience have long sought imaging techniques that can identify and distinguish neural ensembles that are activated by sequentially applied stimuli at single-cell resolution across the whole brain. Taking advantage of the different kinetics of immediate-early genes' mRNA and protein expression, we addressed this problem by developing tyramide-amplified immunohistochemistry-fluorescence in situ hybridization (TAI-FISH), a dual-epoch neural-activity-dependent labeling protocol. Here we describe the step-by-step procedures for TAI-FISH on brain sections from mice that were sequentially stimulated by morphine (appetitive first stimulus) and foot shock (aversive second stimulus). We exemplify our approach by FISH-labeling the neural ensembles that were activated by the second stimulus for the mRNA expression of c-fos, a well-established marker of neural activation. We labeled neuronal ensembles activated by the first stimulus using fluorescence immunohistochemistry (IHC) for the c-fos protein. To further improve the temporal separation of the c-fos mRNA and protein signals, we provide instructions on how to enhance the protein signals using tyramide signal amplification (TSA). Compared with other dual-epoch labeling techniques, TAI-FISH provides better temporal separation of the activated neural ensembles and is better suited to investigation of whole-brain responses. TAI-FISH has been used to investigate neural activation patterns in response to appetitive and aversive stimuli, and we expect it to be more broadly utilized for visualizing brain responses to other types of stimuli, such as sensory stimuli or psychiatric drugs. From first stimulation to image analysis, TAI-FISH takes ∼9 d to complete.
[Mh] Termos MeSH primário: Encéfalo/efeitos dos fármacos
Imuno-Histoquímica/métodos
Hibridização in Situ Fluorescente/métodos
Neurônios/fisiologia
Proteínas Proto-Oncogênicas c-fos/análise
[Mh] Termos MeSH secundário: Animais
Biomarcadores/análise
Encéfalo/fisiologia
Regulação da Expressão Gênica
Genes Precoces
Genes fos
Camundongos
Imagem Molecular/métodos
Morfina/farmacologia
Neurônios/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-fos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Proto-Oncogene Proteins c-fos); 76I7G6D29C (Morphine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.134


  3 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28377404
[Au] Autor:Iwata K; Kunimura Y; Matsumoto K; Ozawa H
[Ad] Endereço:Department of Anatomy and NeurobiologyGraduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan kiwata0309@nms.ac.jp.
[Ti] Título:Effect of androgen on expression and luteinizing hormone release in female rats.
[So] Source:J Endocrinol;233(3):281-292, 2017 Jun.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hyperandrogenic women have various grades of ovulatory dysfunction, which lead to infertility. The purpose of this study was to determine whether chronic exposure to androgen affects the expression of kisspeptin (ovulation and follicle development regulator) or release of luteinizing hormone (LH) in female rats. Weaned females were subcutaneously implanted with 90-day continuous-release pellets of 5α-dihydrotestosterone (DHT) and studied after 10 weeks of age. Number of -expressing cells in both the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC) was significantly decreased in ovary-intact DHT rats. Further, an estradiol-induced LH surge was not detected in DHT rats, even though significant differences were not observed between DHT and non-DHT rats with regard to number of AVPV -expressing cells or gonadotrophin-releasing hormone (GnRH)-immunoreactive (ir) cells in the presence of high estradiol. -expressing and neurokinin B-ir cells were significantly decreased in the ARC of ovariectomized (OVX) DHT rats compared with OVX non-DHT rats; pulsatile LH secretion was also suppressed in these animals. Central injection of kisspeptin-10 or intravenous injection of a GnRH agonist did not affect the LH release in DHT rats. Notably, ARC -expressing cells expressed androgen receptors (ARs) in female rats, whereas only a few -expressing cells expressed ARs in the AVPV. Collectively, our results suggest excessive androgen suppresses LH surge and pulsatile LH secretion by inhibiting kisspeptin expression in the ARC and disruption at the pituitary level, whereas AVPV kisspeptin neurons appear to be directly unaffected by androgen. Hence, hyperandrogenemia may adversely affect ARC kisspeptin neurons, resulting in anovulation and menstrual irregularities.
[Mh] Termos MeSH primário: Di-Hidrotestosterona/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Kisspeptinas/metabolismo
Hormônio Luteinizante/metabolismo
[Mh] Termos MeSH secundário: Animais
Núcleo Arqueado do Hipotálamo/fisiologia
Di-Hidrotestosterona/administração & dosagem
Esquema de Medicação
Feminino
Genes fos/fisiologia
Hipotálamo Anterior/fisiologia
Imuno-Histoquímica
Kisspeptinas/genética
Hormônio Luteinizante/genética
Neurocinina B/metabolismo
RNA Mensageiro
Ratos
Receptores Androgênicos/genética
Receptores Androgênicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Kiss1 protein, rat); 0 (Kisspeptins); 0 (RNA, Messenger); 0 (Receptors, Androgen); 08J2K08A3Y (Dihydrotestosterone); 86933-75-7 (Neurokinin B); 9002-67-9 (Luteinizing Hormone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-16-0568


  4 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28318068
[Au] Autor:Zhang MQ; Wang TX; Li R; Huang ZL; Han WJ; Dai XC; Wang YQ
[Ad] Endereço:Department of Pharmacology and Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China.
[Ti] Título:Helicid alleviates pain and sleep disturbances in a neuropathic pain-like model in mice.
[So] Source:J Sleep Res;26(3):386-393, 2017 Jun.
[Is] ISSN:1365-2869
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Natural helicid (4-formylphenyl-O-ß-d-allopyranoside), a main active constituent from seeds of the Chinese herb Helicia nilagirica, has been reported to exert a sedative, analgesic and hypnotic effect, and is used clinically to treat neurasthenic syndrome, vascular headaches and trigeminal neuralgia. In the current study, mechanical allodynia tests, electroencephalograms, electromyogram recordings and c-Fos expression in neuropathic pain-like model mice of partial sciatic nerve ligation were used to investigate the effect of helicid on neuropathic pain and co-morbid insomnia. Our results showed that helicid at a dose of 100, 200 or 400 mg kg could increase the mechanical threshold by 2.5-, 2.8- and 3.1-fold for 3 h after administration, respectively. Helicid at 200 and 400 mg kg given at 07:00 hours increased the amount of non-rapid eye movement sleep in a 3-h period by 1.27- and 1.35-fold in partial sciatic nerve ligated mice. However, helicid (400 mg kg ) given at 21:00 hours did not change the sleep pattern in normal mice. Immunohistochemical study showed that helicid (400 mg kg ) administration could reverse the increase of c-Fos expression in the neurons of the rostral anterior cingulate cortex and tuberomammillary nucleus, and the decrease of c-Fos expression in the ventrolateral preoptic area caused by partial sciatic nerve ligation. These results indicate that helicid is an effective agent for both neuropathic pain and sleep disturbances in partial sciatic nerve ligated mice.
[Mh] Termos MeSH primário: Benzaldeídos/uso terapêutico
Neuralgia/complicações
Neuralgia/tratamento farmacológico
Distúrbios do Início e da Manutenção do Sono/etiologia
Distúrbios do Início e da Manutenção do Sono/prevenção & controle
[Mh] Termos MeSH secundário: Analgésicos/administração & dosagem
Analgésicos/farmacologia
Analgésicos/uso terapêutico
Animais
Benzaldeídos/administração & dosagem
Benzaldeídos/farmacologia
Modelos Animais de Doenças
Eletroencefalografia
Eletromiografia
Genes fos/genética
Giro do Cíngulo/efeitos dos fármacos
Giro do Cíngulo/metabolismo
Hiperalgesia/diagnóstico
Hipnóticos e Sedativos/administração & dosagem
Hipnóticos e Sedativos/farmacologia
Hipnóticos e Sedativos/uso terapêutico
Região Hipotalâmica Lateral/efeitos dos fármacos
Região Hipotalâmica Lateral/metabolismo
Ligadura
Masculino
Camundongos
Área Pré-Óptica/efeitos dos fármacos
Área Pré-Óptica/metabolismo
Nervo Isquiático
Sono/efeitos dos fármacos
Sono/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Benzaldehydes); 0 (Hypnotics and Sedatives); 80154-34-3 (helicide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1111/jsr.12518


  5 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28255683
[Au] Autor:Cobbina SJ; Mao G; Zhao T; Xu H; Zhang Z; Liu H; Zou Y; Wu X; Yang L
[Ad] Endereço:School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013, Jiangsu, China.
[Ti] Título:Modulation of N-Methyl-D-Aspartate Receptors (NMDAR), Bcl-2 and C-Fos Gene Expressions on Exposure to Individual and Mixtures of Low Concentration Metals in Zebrafish (Danio rerio).
[So] Source:Arch Environ Contam Toxicol;72(3):418-427, 2017 Apr.
[Is] ISSN:1432-0703
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Currently, there is limited information on the toxicity of low concentration of metal mixtures in the environment. Of particular interest is the effect of low levels of metal mixtures on neurodevelopment of aquatic organisms. This study reports the neurological gene expressions after exposing zebrafish embryos to low concentration toxic heavy metals, 120 h post fertilization (hpf). Embryos were exposed to low concentration individual and mixtures of lead (Pb), mercury (Hg), arsenic (As), and cadmium (Cd). Quantitative real-time PCR was used to assess gene expressions. The findings of this study confirmed that exposure to low concentration heavy metals upregulated N-methyl-D-aspartate (NMDA) receptor subunits NMDAR2A (NR2A), NMDAR2B (NR2B), and NMDAR2D (NR2D) and B cell lymphoma (Bcl-2) genes. NR2A genes were significantly upregulated by 90 and 74%, respectively, on exposure to Pb + As and Pb + Cd. NR2B genes were upregulated by 85.3, 68.6, 62.7, and 62.7% on exposure to As, Pb + Hg, Pb + As, and Pb + Cd, respectively. Exposure to As, Pb + Cd, and Pb + Hg + As significantly upregulated Bcl-2 genes by 2.01-, 1.84-, and 1.80-fold, respectively. NR1A and C-fos gene expressions were not significantly different from control. Upregulation of NMDAR subunits and Bcl-2 genes in this study was largely a counter measure against insults from exposure to low concentration heavy metals. Principal component analysis confirmed the influence of low concentration individual and mixtures of Pb, Hg, As, and Cd on gene expression of NMDAR subunits and Bcl-2. These data suggest that altered expression of NMDA receptor subunits and Bcl-2 genes may explain toxicity of low concentration individual and mixtures of Pb, Hg, As, and Cd.
[Mh] Termos MeSH primário: Expressão Gênica/efeitos dos fármacos
Genes bcl-2
Genes fos
Metais/toxicidade
Receptores de N-Metil-D-Aspartato/genética
Poluentes Químicos da Água/toxicidade
Peixe-Zebra/fisiologia
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero/efeitos dos fármacos
Embrião não Mamífero/fisiologia
Peixe-Zebra/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals); 0 (Receptors, N-Methyl-D-Aspartate); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1007/s00244-016-0352-y


  6 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28038985
[Au] Autor:Zhang L; Cui Y; Wang YC; Yin H; Zheng JM; Huang L; Zhao ZW; Li J
[Ad] Endereço:Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China. Electronic address: zhanglei363@163.com.
[Ti] Título:RETRACTED: Exploring the mechanism by which accumbal deep brain stimulation attenuates morphine-induced reinstatement through manganese-enhanced MRI and pharmacological intervention.
[So] Source:Exp Neurol;290:29-40, 2017 04.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the authors. The authors have requested to retract this paper as the corresponding author had not sought the prior agreement of his co-authors to submit the paper for publication.
[Mh] Termos MeSH primário: Estimulação Encefálica Profunda/métodos
Dependência de Morfina/diagnóstico por imagem
Dependência de Morfina/terapia
Núcleo Accumbens
[Mh] Termos MeSH secundário: Animais
Condicionamento Operante/efeitos dos fármacos
Meios de Contraste
Antagonistas GABAérgicos/farmacologia
Genes fos/efeitos dos fármacos
Interneurônios/efeitos dos fármacos
Imagem por Ressonância Magnética/métodos
Masculino
Manganês
Dependência de Morfina/psicologia
Neurônios Aferentes/efeitos dos fármacos
Ratos
Ratos Sprague-Dawley
Recidiva
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 0 (GABA Antagonists); 42Z2K6ZL8P (Manganese); 56-12-2 (gamma-Aminobutyric Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170101
[St] Status:MEDLINE


  7 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27959426
[Au] Autor:Girgert R; Emons G; Gründker C
[Ad] Endereço:Department of Gynecology and Obstetrics, School of Medicine, University of Göttingen, D-37075 Göttingen, Germany.
[Ti] Título:17ß-estradiol-induced growth of triple-negative breast cancer cells is prevented by the reduction of GPER expression after treatment with gefitinib.
[So] Source:Oncol Rep;37(2):1212-1218, 2017 Feb.
[Is] ISSN:1791-2431
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Triple-negative breast cancers (TNBCs) are neither susceptible to endocrine therapy due to a lack of estrogen receptor α expression nor trastuzumab. TNBCs frequently overexpress epidermal growth factor receptor (EGFR) and membrane bound estrogen receptor, GPER. To a certain extent the growth of TNBCs is stimulated by 17ß-estradiol via GPER. We analyzed whether inhibition of EGFR by gefitinib reduces the expression of GPER and subsequent signal transduction in TNBC cells. Dependence of proliferation on 17ß-estradiol was determined using Alamar Blue assay. Expression of GPR30 and activation of c-src, EGFR and cAMP-responsive element binding (CREB) protein by 17ß-estradiol was analyzed by western blotting. Expression of c-fos, cyclin D1 and aromatase was determined using RT-PCR. Gefitinib reduced GPER expression concentration­ and time­dependently. In HCC70 cells, GPER expression was reduced to 15±11% (p<0.05) after treatment with 200 nM gefitinib for four days, and in HCC1806 cells GPER expression was reduced to 39±5% (p<0.01) of the control. 17ß-estradiol significantly increased the percentage of HCC1806 cells within 7 days to 145±29% of the control (HCC70, 110±8%). This increase in cell growth was completely prevented in both TNBC cell lines after GPR30 expression was downregulated by treatment with 200 nM gefitinib. In HCC1806 cells, activation of c-src was increased by 17ß-estradiol to 350±50% (p<0.01), and gefitinib reduced src activation to 110%. Similar results were obtained in the HCC70 cells. Phosphorylation of EGFR increased to 240±40% (p<0.05) in the HCC1806 cells treated with 17ß-estradiol (HCC70, 147±25%). Gefitinib completely prevented this activation. Phosphorylation of CREB and induction of c-fos, cyclin D1 and aromatase expression by 17ß-estradiol were all prevented by gefitinib. These experiments conclusively show that reduction of GPER expression is a promising therapeutic approach for TNBC.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Estradiol/farmacologia
Quinazolinas/farmacologia
Receptores Estrogênicos/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
Neoplasias de Mama Triplo Negativas/tratamento farmacológico
[Mh] Termos MeSH secundário: Aromatase/metabolismo
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Ciclina D1/metabolismo
Feminino
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Genes fos
Seres Humanos
Fosforilação/efeitos dos fármacos
Receptor do Fator de Crescimento Epidérmico/metabolismo
Neoplasias de Mama Triplo Negativas/genética
Neoplasias de Mama Triplo Negativas/metabolismo
Neoplasias de Mama Triplo Negativas/patologia
Quinases da Família src/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (CCND1 protein, human); 0 (GPER protein, human); 0 (Quinazolines); 0 (Receptors, Estrogen); 0 (Receptors, G-Protein-Coupled); 136601-57-5 (Cyclin D1); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.10.2 (CSK tyrosine-protein kinase); EC 2.7.10.2 (src-Family Kinases); S65743JHBS (gefitinib)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170323
[Lr] Data última revisão:
170323
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.3892/or.2016.5306


  8 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27174774
[Au] Autor:Santos P; da Silva LE; Leão RM
[Ad] Endereço:Universidade de São Paulo (USP), Faculdade de Medicina Ribeirão Preto (FMRP), Departamento de Oftalmologia e Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Ribeirão Preto, SP, Brazil; Universidade de São Paulo (USP), Faculdade de Medicina Ribeirão Preto (FMRP), Departamento de Fisiologia, Ribeirão Preto, SP, Brazil.
[Ti] Título:Specific immediate early gene expression induced by high doses of salicylate in the cochlear nucleus and inferior colliculus of the rat.
[So] Source:Braz J Otorhinolaryngol;83(2):155-161, 2017 Mar - Apr.
[Is] ISSN:1808-8686
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Salicylate at high doses induces tinnitus in humans and experimental animals. However, the mechanisms and loci of action of salicylate in inducing tinnitus are still not well known. The expression of Immediate Early Genes (IEG) is traditionally associated with long-term neuronal modifications but it is still not clear how and where IEGs are activated in animal models of tinnitus. OBJECTIVES: Here we investigated the expression of c-fos and Egr-1, two IEGs, in the Dorsal Cochlear Nucleus (DCN), the Inferior Colliculus (IC), and the Posterior Ventral Cochlear Nucleus (pVCN) of rats. METHODS: Rats were treated with doses known to induce tinnitus in rats (300mg/kg i.p. daily, for 3 days), and c-fos and Egr-1 protein expressions were analyzed using western blot and immunocytochemistry. RESULTS: After administration of salicylate, c-fos protein expression increased significantly in the DCN, pVCN and IC when assayed by western blot. Immunohistochemistry staining showed a more intense labeling of c-fos in the DCN, pVCN and IC and a significant increase in c-fos positive nuclei in the pVCN and IC. We did not detect increased Egr-1 expression in any of these areas. CONCLUSION: Our data show that a high dose of salicylate activates neurons in the DCN, pVCN and IC. The expression of these genes by high doses of salicylate strongly suggests that plastic changes in these areas are involved in the genesis of tinnitus.
[Mh] Termos MeSH primário: Núcleo Coclear/efeitos dos fármacos
Regulação da Expressão Gênica/efeitos dos fármacos
Genes Precoces/efeitos dos fármacos
Colículos Inferiores/efeitos dos fármacos
Salicilatos/farmacologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Relação Dose-Resposta a Droga
Proteína 1 de Resposta de Crescimento Precoce/efeitos dos fármacos
Genes fos/efeitos dos fármacos
Masculino
Ratos
Ratos Wistar
Salicilatos/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (EGR1 protein, human); 0 (Early Growth Response Protein 1); 0 (Salicylates)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160514
[St] Status:MEDLINE


  9 / 4402 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27889766
[Au] Autor:Sotelo MI; Daneri MF; Bingman VP; Muzio RN
[Ad] Endereço:Grupo de Aprendizaje y Cognición Comparada, Laboratorio de Biología del Comportamiento, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.
[Ti] Título:Telencephalic Neuronal Activation Associated with Spatial Memory in the Terrestrial Toad Rhinella arenarum: Participation of the Medial Pallium during Navigation by Geometry.
[So] Source:Brain Behav Evol;88(3-4):149-160, 2016.
[Is] ISSN:1421-9743
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Amphibians are central to discussions of vertebrate evolution because they represent the transition from aquatic to terrestrial life, a transition with profound consequences for the selective pressures shaping brain evolution. Spatial navigation is one class of behavior that has attracted the interest of comparative neurobiologists because of the relevance of the medial pallium/hippocampus, yet, surprisingly, in this regard amphibians have been sparsely investigated. In the current study, we trained toads to locate a water goal relying on the boundary geometry of a test environment (Geometry-Only) or boundary geometry coupled with a prominent, visual feature cue (Geometry-Feature). Once learning had been achieved, the animals were given one last training session and their telencephali were processed for c-Fos activation. Compared to control toads exposed to the test environment for the first time, geometry-only toads were found to have increased neuronal labeling in the medial pallium, the presumptive hippocampal homologue, while geometry-feature toads were found to have increased neuronal labeling in the medial, dorsal, and lateral pallia. The data indicate medial pallial participation in guiding navigation by environmental geometry and lateral, and to a lesser extent dorsal, pallial participation in guiding navigation by a prominent visual feature. As such, participation of the medial pallium/hippocampus in spatial cognition appears to be a conserved feature of terrestrial vertebrates even if their life history is still tied to water, a brain-behavior feature seemingly at least as ancient as the evolutionary transition to life on land.
[Mh] Termos MeSH primário: Bufonidae/fisiologia
Neurônios/fisiologia
Aprendizagem Espacial/fisiologia
Memória Espacial/fisiologia
Navegação Espacial/fisiologia
Telencéfalo/fisiologia
[Mh] Termos MeSH secundário: Animais
Argentina
Genes fos
Imuno-Histoquímica
Telencéfalo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE
[do] DOI:10.1159/000447441


  10 / 4402 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27476939
[Au] Autor:Kono Y; Iwasaki A; Matsuoka K; Fujita T
[Ad] Endereço:Laboratory of Molecular Pharmacokinetics, College of Pharmaceutical Sciences, Ritsumeikan University.
[Ti] Título:Effect of Mechanical Agitation on Cationic Liposome Transport across an Unstirred Water Layer in Caco-2 Cells.
[So] Source:Biol Pharm Bull;39(8):1293-9, 2016.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To develop an effective oral delivery system for plasmid DNA (pDNA) using cationic liposomes, it is necessary to clarify the characteristics of uptake and transport of cationic liposome/pDNA complexes into the intestinal epithelium. In particular, evaluation of the involvement of an unstirred water layer (UWL), which is a considerable permeability barrier, in cationic liposome transport is very important. Here, we investigated the effects of a UWL on the transfection efficiency of cationic liposome/pDNA complexes into a Caco-2 cell monolayer. When Caco-2 cells were transfected with cationic liposome/pDNA complexes in shaking cultures to reduce the thickness of the UWL, gene expression was significantly higher in Caco-2 cells compared with static cultures. We also found that this enhancement of gene expression by shaking was not attributable to activation of transcription factors such as activator protein-1 and nuclear factor-kappaB (NF-κB). In addition, the increase in gene expression by mechanical agitation was observed at all charge ratios (1.5, 2.3, 3.1, 4.5) of cationic liposome/pDNA complexes. Transport experiments using Transwells demonstrated that mechanical agitation increased the uptake of cationic liposome/pDNA complexes by Caco-2 cells, whereas transport of the complexes across a Caco-2 cell monolayer did not occurr. Moreover, the augmentation of the gene expression of cationic liposome/pDNA complexes by shaking was observed in Madin-Darby canine kidney cells. These results indicate that a UWL greatly affects the uptake and transfection efficiency of cationic liposome/pDNA complexes into an epithelial monolayer in vitro.
[Mh] Termos MeSH primário: DNA/administração & dosagem
Lipossomos/administração & dosagem
Transfecção/métodos
[Mh] Termos MeSH secundário: Animais
Células CACO-2
Cátions
Cães
Expressão Gênica
Genes fos/genética
Seres Humanos
Células Madin Darby de Rim Canino
Plasmídeos
Proteínas Proto-Oncogênicas c-jun/genética
RNA Mensageiro/metabolismo
Fator de Transcrição RelA/metabolismo
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Liposomes); 0 (Proto-Oncogene Proteins c-jun); 0 (RNA, Messenger); 0 (Transcription Factor RelA); 059QF0KO0R (Water); 9007-49-2 (DNA)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b16-00050



página 1 de 441 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde