Base de dados : MEDLINE
Pesquisa : G05.360.340.337 [Categoria DeCS]
Referências encontradas : 595 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 60 ir para página                         

  1 / 595 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28837599
[Au] Autor:Brunquell J; Snyder A; Cheng F; Westerheide SD
[Ad] Endereço:Department of Cell Biology, Microbiology, and Molecular Biology, College of Arts and Sciences, University of South Florida, Tampa, Florida, United States of America.
[Ti] Título:HSF-1 is a regulator of miRNA expression in Caenorhabditis elegans.
[So] Source:PLoS One;12(8):e0183445, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability of an organism to sense and adapt to environmental stressors is essential for proteome maintenance and survival. The highly conserved heat shock response is a survival mechanism employed by all organisms, including the nematode Caenorhabditis elegans, upon exposure to environmental extremes. Transcriptional control of the metazoan heat shock response is mediated by the heat shock transcription factor HSF-1. In addition to regulating global stress-responsive genes to promote stress-resistance and survival, HSF-1 has recently been shown to regulate stress-independent functions in controlling development, metabolism, and longevity. However, the indirect role of HSF-1 in coordinating stress-dependent and -independent processes through post-transcriptional regulation is largely unknown. MicroRNAs (miRNAs) have emerged as a class of post-transcriptional regulators that control gene expression through translational repression or mRNA degradation. To determine the role of HSF-1 in regulating miRNA expression, we have performed high-throughput small RNA-sequencing in C. elegans grown in the presence and absence of hsf-1 RNAi followed by treatment with or without heat shock. This has allowed us to uncover the miRNAs regulated by HSF-1 via heat-dependent and -independent mechanisms. Integrated miRNA/mRNA target-prediction analyses suggest HSF-1 as a post-transcriptional regulator of development, metabolism, and longevity through regulating miRNA expression. This provides new insight into the possible mechanism by which HSF-1 controls these processes. We have also uncovered oxidative stress response factors and insulin-like signaling factors as a common link between processes affected by HSF-1-regulated miRNAs in stress-dependent and -independent mechanisms, respectively. This may provide a role for miRNAs in regulating cross-talk between various stress responses. Our work therefore uncovers an interesting potential role for HSF-1 in post-transcriptionally controlling gene expression in C. elegans, and suggests a mechanism for cross-talk between stress responses.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/genética
Caenorhabditis elegans/genética
MicroRNAs/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Animais
Genoma Helmíntico
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (MicroRNAs); 0 (Transcription Factors); 0 (heat shock factor-1, C elegans)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183445


  2 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28673184
[Au] Autor:Gonzalez-Moragas L; Yu SM; Benseny-Cases N; Stürzenbaum S; Roig A; Laromaine A
[Ad] Endereço:a Group of Nanoparticles and Nanocomposites, Crystallography Department , Institut de Ciència de Materials de Barcelona, ICMAB-CSIC , Barcelona , Campus UAB , Spain.
[Ti] Título:Toxicogenomics of iron oxide nanoparticles in the nematode C. elegans.
[So] Source:Nanotoxicology;11(5):647-657, 2017 Jun.
[Is] ISSN:1743-5404
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We present a mechanistic study of the effect of iron oxide nanoparticles (SPIONs) in Caenorhabditis elegans combining a genome-wide analysis with the investigation of specific molecular markers frequently linked to nanotoxicity. The effects of two different coatings were explored: citrate, an anionic stabilizer, and bovine serum albumin, as a pre-formed protein corona. The transcriptomic study identified differentially expressed genes following an exposure to SPIONs. The expression of genes involved in oxidative stress, metal detoxification response, endocytosis, intestinal integrity and iron homeostasis was quantitatively evaluated. The role of oxidative stress was confirmed by gene expression analysis and by synchrotron Fourier Transform infrared microscopy based on the higher tissue oxidation of NP-treated animals. The observed transcriptional modulation of key signaling pathways such as MAPK and Wnt suggests that SPIONs might be endocytosed by clathrin-mediated processes, a putative mechanism of nanotoxicity which deserves further mechanistic investigations.
[Mh] Termos MeSH primário: Caenorhabditis elegans
Nanopartículas de Magnetita/toxicidade
Toxicogenética/métodos
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/efeitos dos fármacos
Caenorhabditis elegans/genética
Endocitose/efeitos dos fármacos
Endocitose/genética
Genoma Helmíntico
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/genética
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Magnetite Nanoparticles)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE
[do] DOI:10.1080/17435390.2017.1342011


  3 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28634273
[Au] Autor:Cottee PA; Cole T; Schultz J; Hoang HD; Vibbert J; Han SM; Miller MA
[Ad] Endereço:Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
[Ti] Título:The VAPB homolog VPR-1 is a permissive signal for gonad development.
[So] Source:Development;144(12):2187-2199, 2017 06 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:VAMP/synaptobrevin-associated proteins (VAPs) contain an N-terminal major sperm protein domain (MSPd) that is associated with amyotrophic lateral sclerosis. VAPs have an intracellular housekeeping function, as well as an extracellular signaling function mediated by the secreted MSPd. Here we show that the VAP homolog VPR-1 is essential for gonad development. null mutants are maternal effect sterile due to arrested gonadogenesis following embryo hatching. Somatic gonadal precursor cells and germ cells fail to proliferate fully and complete their respective differentiation programs. Maternal or zygotic expression is sufficient to induce gonadogenesis and fertility. Genetic mosaic and cell type-specific expression studies indicate that activity is important in the nervous system, germ line and intestine. VPR-1 acts in parallel to Notch signaling, a key regulator of germline stem cell proliferation and differentiation. Neuronal expression is sufficient for gonadogenesis induction during a limited time period shortly after hatching. These results support the model that the secreted VPR-1 MSPd acts at least in part on gonadal sheath cell precursors in L1 to early L2 stage hermaphrodites to permit gonadogenesis.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/metabolismo
Gônadas/crescimento & desenvolvimento
Gônadas/metabolismo
Proteínas de Membrana/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/genética
Diferenciação Celular
Feminino
Técnicas de Inativação de Genes
Genoma Helmíntico
Células Germinativas/citologia
Células Germinativas/metabolismo
Intestinos/crescimento & desenvolvimento
Intestinos/metabolismo
Masculino
Proteínas de Membrana/deficiência
Proteínas de Membrana/genética
Modelos Biológicos
Mosaicismo
Neurogênese
Organogênese
Receptores Notch/genética
Receptores Notch/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Glp-1 protein, C elegans); 0 (Membrane Proteins); 0 (Receptors, Notch); 0 (VPR-1 protein, C elegans)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1242/dev.152207


  4 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28594822
[Au] Autor:Blanc-Mathieu R; Perfus-Barbeoch L; Aury JM; Da Rocha M; Gouzy J; Sallet E; Martin-Jimenez C; Bailly-Bechet M; Castagnone-Sereno P; Flot JF; Kozlowski DK; Cazareth J; Couloux A; Da Silva C; Guy J; Kim-Jo YJ; Rancurel C; Schiex T; Abad P; Wincker P; Danchin EGJ
[Ad] Endereço:INRA, Université Côte d'Azur, CNRS, ISA, France.
[Ti] Título:Hybridization and polyploidy enable genomic plasticity without sex in the most devastating plant-parasitic nematodes.
[So] Source:PLoS Genet;13(6):e1006777, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Root-knot nematodes (genus Meloidogyne) exhibit a diversity of reproductive modes ranging from obligatory sexual to fully asexual reproduction. Intriguingly, the most widespread and devastating species to global agriculture are those that reproduce asexually, without meiosis. To disentangle this surprising parasitic success despite the absence of sex and genetic exchanges, we have sequenced and assembled the genomes of three obligatory ameiotic and asexual Meloidogyne. We have compared them to those of relatives able to perform meiosis and sexual reproduction. We show that the genomes of ameiotic asexual Meloidogyne are large, polyploid and made of duplicated regions with a high within-species average nucleotide divergence of ~8%. Phylogenomic analysis of the genes present in these duplicated regions suggests that they originated from multiple hybridization events and are thus homoeologs. We found that up to 22% of homoeologous gene pairs were under positive selection and these genes covered a wide spectrum of predicted functional categories. To biologically assess functional divergence, we compared expression patterns of homoeologous gene pairs across developmental life stages using an RNAseq approach in the most economically important asexually-reproducing nematode. We showed that >60% of homoeologous gene pairs display diverged expression patterns. These results suggest a substantial functional impact of the genome structure. Contrasting with high within-species nuclear genome divergence, mitochondrial genome divergence between the three ameiotic asexuals was very low, signifying that these putative hybrids share a recent common maternal ancestor. Transposable elements (TE) cover a ~1.7 times higher proportion of the genomes of the ameiotic asexual Meloidogyne compared to the sexual relative and might also participate in their plasticity. The intriguing parasitic success of asexually-reproducing Meloidogyne species could be partly explained by their TE-rich composite genomes, resulting from allopolyploidization events, and promoting plasticity and functional divergence between gene copies in the absence of sex and meiosis.
[Mh] Termos MeSH primário: Variação Genética
Genoma Helmíntico
Hibridização Genética
Poliploidia
Reprodução Assexuada
Tylenchoidea/genética
[Mh] Termos MeSH secundário: Animais
Elementos de DNA Transponíveis
Genoma Mitocondrial
Polimorfismo Genético
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006777


  5 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28537229
[Au] Autor:Chelomina GN
[Ad] Endereço:Institute of Biology and Soil Science, Far East Branch, Russian Academy of Sciences, Vladivostok, 690022 Russia.
[Ti] Título:[Genomics and transcriptomics of the Chinese liver fluke Clonorchis sinensis (Opisthorchiidae, Trematoda)].
[So] Source:Mol Biol (Mosk);51(2):215-226, 2017 Mar-Apr.
[Is] ISSN:0026-8984
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The review summarizes the results of first genomic and transcriptomic investigations of the liver fluke Clonorchis sinensis (Opisthorchiidae, Trematoda). The studies mark the dawn of the genomic era for opisthorchiids, which cause severe hepatobiliary diseases in humans and animals. Their results aided in understanding the molecular mechanisms of adaptation to parasitism, parasite survival in mammalian biliary tracts, and genome dynamics in the individual development and the development of parasite-host relationships. Special attention is paid to the achievements in studying the codon usage bias and the roles of mobile genetic elements (MGEs) and small interfering RNAs (siRNAs). Interspecific comparisons at the genomic and transcriptomic levels revealed molecular differences, which may contribute to understanding the specialized niches and physiological needs of the respective species. The studies in C. sinensis provide a basis for further basic and applied research in liver flukes and, in particular, the development of efficient means to prevent, diagnose, and treat clonorchiasis.
[Mh] Termos MeSH primário: Adaptação Biológica/fisiologia
Clonorchis sinensis/genética
Genoma Helmíntico/fisiologia
Transcriptoma/fisiologia
[Mh] Termos MeSH secundário: Animais
Clonorquíase/genética
Clonorquíase/metabolismo
Clonorquíase/terapia
Clonorchis sinensis/metabolismo
Perfilação da Expressão Gênica
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.7868/S0026898417020070


  6 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28506040
[Au] Autor:Bae YA
[Ad] Endereço:Department of Microbiology, Gachon University College of Medicine, Incheon 21936, Korea.
[Ti] Título:Codon Usage Patterns of Tyrosinase Genes in .
[So] Source:Korean J Parasitol;55(2):175-183, 2017 Apr.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Codon usage bias (CUB) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. In this study, genetic indices associated with CUB, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. The relative synonymous codon usage patterns substantially differed among tyrosinase genes examined. In a neutrality analysis, the correlation between GC and GC was statistically significant, and the regression line had a relatively gradual slope (0.218). NC-plot, i.e., GC vs effective number of codons (ENC), showed that most of the tyrosinase genes were below the expected curve. The codon adaptation index (CAI) values of the platyhelminth tyrosinases had a narrow distribution between 0.685/0.714 and 0.797/0.837, and were negatively correlated with their ENC. Taken together, these results suggested that CUB in the tyrosinase genes seemed to be basically governed by selection pressures rather than mutational bias, although the latter factor provided an additional force in shaping CUB of the and genes. It was also apparent that the equilibrium point between selection pressure and mutational bias is much more inclined to selection pressure in highly expressed genes, than in poorly expressed genes.
[Mh] Termos MeSH primário: Clonorchis sinensis/enzimologia
Clonorchis sinensis/genética
Códon/genética
Genoma Helmíntico/genética
Monofenol Mono-Oxigenase/genética
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Códon/química
Evolução Molecular
Monofenol Mono-Oxigenase/fisiologia
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); EC 1.14.18.1 (Monophenol Monooxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.2.175


  7 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28433562
[Au] Autor:Feng Y; Feng HL; Fang YH; Su YB
[Ad] Endereço:School of Urban Construction, Yangtze University, Jingzhou 434023, Hubei, PR China. Electronic address: 785609881@qq.com.
[Ti] Título:Characterization of the complete mitochondrial genome of Khawia sinensis belongs among platyhelminths, cestodes.
[So] Source:Exp Parasitol;177:35-39, 2017 Jun.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Khawia sinensis is an important species in freshwater fish causing considerable economic losses to the breeding industry. This is the first mt genome of a caryophyllidean cestode characterised. The entire mt genome of K. sinensis is 13,759 bp in length. This mt genome contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two non-coding regions. The arrangement of the K. sinensis mt genome is the same as other tapeworms, however, the incomplete stop codon (A) is more frequent that other species. Phylogenetic analyses based on concatenated amino-acid sequences of the 12 protein-coding genes of 17 tapeworms including K. sinensis were conducted to assess the relationship of K. sinensis with other species, the result indicated K. sinensis was closely related with cestode species. This complete mt genome of K. sinensis will enrich the mitochondrial genome databases of tapeworms and provide important molecular markers for ecology, diagnostics, population variation and evolution of K. sinensis and other species.
[Mh] Termos MeSH primário: Cestoides/classificação
Cestoides/genética
Genoma Helmíntico
Genoma Mitocondrial
[Mh] Termos MeSH secundário: Animais
Infecções por Cestoides/parasitologia
Infecções por Cestoides/veterinária
China
Doenças dos Peixes/parasitologia
Água Doce
Genes de RNAr/genética
Carpa Dourada/parasitologia
Proteínas de Helminto/genética
Intestinos/parasitologia
Filogenia
RNA de Transferência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Helminth Proteins); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE


  8 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28406899
[Au] Autor:Anderson L; Gomes MR; daSilva LF; Pereira ADSA; Mourão MM; Romier C; Pierce R; Verjovski-Almeida S
[Ad] Endereço:Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
[Ti] Título:Histone deacetylase inhibition modulates histone acetylation at gene promoter regions and affects genome-wide gene transcription in Schistosoma mansoni.
[So] Source:PLoS Negl Trop Dis;11(4):e0005539, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Schistosomiasis is a parasitic disease infecting hundreds of millions of people worldwide. Treatment depends on a single drug, praziquantel, which kills the Schistosoma spp. parasite only at the adult stage. HDAC inhibitors (HDACi) such as Trichostatin A (TSA) induce parasite mortality in vitro (schistosomula and adult worms), however the downstream effects of histone hyperacetylation on the parasite are not known. METHODOLOGY/PRINCIPAL FINDINGS: TSA treatment of adult worms in vitro increased histone acetylation at H3K9ac and H3K14ac, which are transcription activation marks, not affecting the unrelated transcription repression mark H3K27me3. We investigated the effect of TSA HDACi on schistosomula gene expression at three different time points, finding a marked genome-wide change in the transcriptome profile. Gene transcription activity was correlated with changes on the chromatin acetylation mark at gene promoter regions. Moreover, combining expression data with ChIP-Seq public data for schistosomula, we found that differentially expressed genes having the H3K4me3 mark at their promoter region in general showed transcription activation upon HDACi treatment, compared with those without the mark, which showed transcription down-regulation. Affected genes are enriched for DNA replication processes, most of them being up-regulated. Twenty out of 22 genes encoding proteins involved in reducing reactive oxygen species accumulation were down-regulated. Dozens of genes encoding proteins with histone reader motifs were changed, including SmEED from the PRC2 complex. We targeted SmEZH2 methyltransferase PRC2 component with a new EZH2 inhibitor (GSK343) and showed a synergistic effect with TSA, significantly increasing schistosomula mortality. CONCLUSIONS/SIGNIFICANCE: Genome-wide gene expression analyses have identified important pathways and cellular functions that were affected and may explain the schistosomicidal effect of TSA HDACi. The change in expression of dozens of histone reader genes involved in regulation of the epigenetic program in S. mansoni can be used as a starting point to look for possible novel schistosomicidal targets.
[Mh] Termos MeSH primário: Inibidores de Histona Desacetilases/farmacologia
Histonas/genética
Ácidos Hidroxâmicos/farmacologia
Indazóis/farmacologia
Piridonas/farmacologia
Schistosoma mansoni/efeitos dos fármacos
Schistosoma mansoni/genética
[Mh] Termos MeSH secundário: Acetilação
Animais
Cromatina/genética
Replicação do DNA
Regulação para Baixo
Feminino
Genoma Helmíntico
Seres Humanos
Masculino
Simulação de Acoplamento Molecular
Regiões Promotoras Genéticas
Transcriptoma
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (GSK343); 0 (Histone Deacetylase Inhibitors); 0 (Histones); 0 (Hydroxamic Acids); 0 (Indazoles); 0 (Pyridones); 3X2S926L3Z (trichostatin A)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005539


  9 / 595 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28319011
[Au] Autor:Wit J; Gilleard JS
[Ad] Endereço:Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada. Electronic address: jwit@ucalgary.ca.
[Ti] Título:Resequencing Helminth Genomes for Population and Genetic Studies.
[So] Source:Trends Parasitol;33(5):388-399, 2017 05.
[Is] ISSN:1471-5007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Next-generation sequencing has become increasingly accessible and economical, making genome-wide studies routine for many species, including humans, model organisms, and domestic livestock. However, in the case of helminth parasites, there are still major practical challenges to the application of these approaches for genetic and population studies. Dozens to hundreds of individual parasites from multiple populations may need to be re-sequenced which, together with the relatively large size of helminth genomes, can still make whole-genome resequencing of individual parasites unfeasible for many studies. Fortunately, there are alternative approaches to the complete sequencing of genomes when conducting genome-wide studies. Here we review some strategies, including genome subsampling and pooling, that enable genome-wide analysis of large numbers of parasites in populations.
[Mh] Termos MeSH primário: Genoma Helmíntico/genética
Análise de Sequência de DNA/normas
[Mh] Termos MeSH secundário: Animais
Genética Populacional/tendências
Estudo de Associação Genômica Ampla
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE


  10 / 595 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28274802
[Au] Autor:Viney M
[Ad] Endereço:School of Biological Sciences, University of Bristol, Tyndall Avenue, Bristol, BS8 1TQ, UK. Electronic address: Mark.Viney@bristol.ac.uk.
[Ti] Título:How Can We Understand the Genomic Basis of Nematode Parasitism?
[So] Source:Trends Parasitol;33(6):444-452, 2017 06.
[Is] ISSN:1471-5007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nematodes are very common animals and they have repeatedly evolved parasitic lifestyles during their evolutionary history. Recently, the genomes of many nematodes, especially parasitic species, have been determined, potentially giving an insight into the genetic and genomic basis of nematodes' parasitism. But, to achieve this, phylogenetically appropriate comparisons of genomes of free-living and parasitic species are needed. Achieving this has often been hampered by the relative lack of information about key free-living species. While such comparative approaches will eventually succeed, I suggest that a synthetic biology approach - moving free-living nematodes towards a parasitic lifestyle - will be our ultimate test of truly understanding the genetic and genomic basis of nematode parasitism.
[Mh] Termos MeSH primário: Evolução Biológica
Genoma Helmíntico/genética
Nematoides/genética
Infecções por Nematoides/parasitologia
[Mh] Termos MeSH secundário: Animais
Genômica
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE



página 1 de 60 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde