Base de dados : MEDLINE
Pesquisa : G05.360.340.370 [Categoria DeCS]
Referências encontradas : 262 [refinar]
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  1 / 262 MEDLINE  
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[PMID]:28763486
[Au] Autor:Asaf S; Khan AL; Aaqil Khan M; Muhammad Imran Q; Kang SM; Al-Hosni K; Jeong EJ; Lee KE; Lee IJ
[Ad] Endereço:School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
[Ti] Título:Comparative analysis of complete plastid genomes from wild soybean (Glycine soja) and nine other Glycine species.
[So] Source:PLoS One;12(8):e0182281, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The plastid genomes of different plant species exhibit significant variation, thereby providing valuable markers for exploring evolutionary relationships and population genetics. Glycine soja (wild soybean) is recognized as the wild ancestor of cultivated soybean (G. max), representing a valuable genetic resource for soybean breeding programmes. In the present study, the complete plastid genome of G. soja was sequenced using Illumina paired-end sequencing and then compared it for the first time with previously reported plastid genome sequences from nine other Glycine species. The G. soja plastid genome was 152,224 bp in length and possessed a typical quadripartite structure, consisting of a pair of inverted repeats (IRa/IRb; 25,574 bp) separated by small (178,963 bp) and large (83,181 bp) single-copy regions, with a 51-kb inversion in the large single-copy region. The genome encoded 134 genes, including 87 protein-coding genes, eight ribosomal RNA genes, and 39 transfer RNA genes, and possessed 204 randomly distributed microsatellites, including 15 forward, 25 tandem, and 34 palindromic repeats. Whole-plastid genome comparisons revealed an overall high degree of sequence similarity between G. max and G. gracilis and some divergence in the intergenic spacers of other species. Greater numbers of indels and SNP substitutions were observed compared with G. cyrtoloba. The sequence of the accD gene from G. soja was highly divergent from those of the other species except for G. max and G. gracilis. Phylogenomic analyses of the complete plastid genomes and 76 shared genes yielded an identical topology and indicated that G. soja is closely related to G. max and G. gracilis. The complete G. soja genome sequenced in the present study is a valuable resource for investigating the population and evolutionary genetics of Glycine species and can be used to identify related species.
[Mh] Termos MeSH primário: Cloroplastos/genética
Genomas de Plastídeos
Feijão de Soja/genética
[Mh] Termos MeSH secundário: Evolução Molecular
Marcadores Genéticos
Variação Genética
Genética Populacional
Repetições de Microssatélites
Filogenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182281


  2 / 262 MEDLINE  
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[PMID]:28601077
[Au] Autor:Yurina NP; Sharapova LS; Odintsova MS
[Ad] Endereço:Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia. nyurina@inbi.ras.ru.
[Ti] Título:Structure of Plastid Genomes of Photosynthetic Eukaryotes.
[So] Source:Biochemistry (Mosc);82(6):678-691, 2017 Jun.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This review presents current views on the plastid genomes of higher plants and summarizes data on the size, structural organization, gene content, and other features of plastid DNAs. Special emphasis is placed on the properties of organization of land plant plastid genomes (nucleoids) that distinguish them from bacterial genomes. The prospects of genetic engineering of chloroplast genomes are discussed.
[Mh] Termos MeSH primário: DNA de Plantas/genética
Genomas de Plastídeos
Plastídeos/genética
[Mh] Termos MeSH secundário: Engenharia Genética/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170612
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917060049


  3 / 262 MEDLINE  
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[PMID]:28575007
[Au] Autor:Stoll A; Harpke D; Schütte C; Stefanczyk N; Brandt R; Blattner FR; Quandt D
[Ad] Endereço:Centro de Estudios Avanzados en Zonas Áridas (CEAZA)-Universidad La Serena, La Serena, Chile.
[Ti] Título:Development of microsatellite markers and assembly of the plastid genome in Cistanthe longiscapa (Montiaceae) based on low-coverage whole genome sequencing.
[So] Source:PLoS One;12(6):e0178402, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cistanthe longiscapa is an endemic annual herb and characteristic element of the Chilean Atacama Desert. Principal threats are the destruction of its seed deposits by human activities and reduced germination rates due to the decreasing occurrence of precipitation events. To enable population genetic and phylogeographic analyses in this species we performed paired-end shotgun sequencing (2x100 bp) of genomic DNA on the Illumina HiSeq platform and identified microsatellite (SSR) loci in the resulting sequences. From 29 million quality-filtered read pairs we obtained 549,174 contigs (average length 614 bp; N50 = 904). Searching for SSRs revealed 10,336 loci with microsatellite motifs. Initially, we designed primers for 96 loci, which were tested for PCR amplification on three C. longiscapa individuals. Successfully amplifying loci were further tested on eight individuals to screen for length variation in the resulting amplicons, and the alleles were exemplarily sequenced to infer the basis for the observed length variation. Finally we arrived at 26 validated SSR loci for population studies in C. longiscapa, which resulted in 146 bi-allelic SSR markers in our test sample of eight individuals. The genomic sequences were also used to assemble the plastid genome of C. longiscapa, which provides an additional set of maternally inherited genetic markers.
[Mh] Termos MeSH primário: Genomas de Plastídeos
Magnoliopsida/genética
Repetições de Microssatélites
[Mh] Termos MeSH secundário: Alelos
Chile
DNA de Plantas/genética
Genoma de Planta
Sequenciamento de Nucleotídeos em Larga Escala
Polimorfismo Genético
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178402


  4 / 262 MEDLINE  
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[PMID]:28444372
[Au] Autor:Hazkani-Covo E; Martin WF
[Ad] Endereço:Department of Natural and Life Sciences, The Open University of Israel, Ra'anana, Israel.
[Ti] Título:Quantifying the Number of Independent Organelle DNA Insertions in Genome Evolution and Human Health.
[So] Source:Genome Biol Evol;9(5):1190-1203, 2017 May 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fragments of organelle genomes are often found as insertions in nuclear DNA. These fragments of mitochondrial DNA (numts) and plastid DNA (nupts) are ubiquitous components of eukaryotic genomes. They are, however, often edited out during the genome assembly process, leading to systematic underestimation of their frequency. Numts and nupts, once inserted, can become further fragmented through subsequent insertion of mobile elements or other recombinational events that disrupt the continuity of the inserted sequence relative to the genuine organelle DNA copy. Because numts and nupts are typically identified through sequence comparison tools such as BLAST, disruption of insertions into smaller fragments can lead to systematic overestimation of numt and nupt frequencies. Accurate identification of numts and nupts is important, however, both for better understanding of their role during evolution, and for monitoring their increasingly evident role in human disease. Human populations are polymorphic for 141 numt loci, five numts are causal to genetic disease, and cancer genomic studies are revealing an abundance of numts associated with tumor progression. Here, we report investigation of salient parameters involved in obtaining accurate estimates of numt and nupt numbers in genome sequence data. Numts and nupts from 44 sequenced eukaryotic genomes reveal lineage-specific differences in the number, relative age and frequency of insertional events as well as lineage-specific dynamics of their postinsertional fragmentation. Our findings outline the main technical parameters influencing accurate identification and frequency estimation of numts in genomic studies pertinent to both evolution and human health.
[Mh] Termos MeSH primário: Elementos de DNA Transponíveis
DNA Mitocondrial/genética
Eucariotos/genética
Evolução Molecular
Plastídeos/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Núcleo Celular/genética
Biologia Computacional/métodos
Genoma Mitocondrial
Genomas de Plastídeos
Seres Humanos
Mitocôndrias/genética
Mutagênese Insercional
Filogenia
Plantas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Mitochondrial)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx078


  5 / 262 MEDLINE  
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[PMID]:28400415
[Au] Autor:Ruhlman TA; Zhang J; Blazier JC; Sabir JSM; Jansen RK
[Ad] Endereço:Department of Integrative Biology, University of Texas at Austin, Austin, Texas 78712 USA truhlman@austin.utexas.edu.
[Ti] Título:Recombination-dependent replication and gene conversion homogenize repeat sequences and diversify plastid genome structure.
[So] Source:Am J Bot;104(4):559-572, 2017 Apr.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: There is a misinterpretation in the literature regarding the variable orientation of the small single copy region of plastid genomes (plastomes). The common phenomenon of small and large single copy inversion, hypothesized to occur through intramolecular recombination between inverted repeats (IR) in a circular, single unit-genome, in fact, more likely occurs through recombination-dependent replication (RDR) of linear plastome templates. If RDR can be primed through both intra- and intermolecular recombination, then this mechanism could not only create inversion isomers of so-called single copy regions, but also an array of alternative sequence arrangements. METHODS: We used Illumina paired-end and PacBio single-molecule real-time (SMRT) sequences to characterize repeat structure in the plastome of (Geraniaceae). We used OrgConv and inspected nucleotide alignments to infer ancestral nucleotides and identify gene conversion among repeats and mapped long (>1 kb) SMRT reads against the unit-genome assembly to identify alternative sequence arrangements. RESULTS: Although lacks the canonical IR, we found that large repeats (>1 kilobase; kb) represent ∼22% of the plastome nucleotide content. Among the largest repeats (>2 kb), we identified GC-biased gene conversion and mapping filtered, long SMRT reads to the unit-genome assembly revealed alternative, substoichiometric sequence arrangements. CONCLUSION: We offer a model based on RDR and gene conversion between long repeated sequences in the plastome and provide support that both intra-and intermolecular recombination between large repeats, particularly in repeat-rich plastomes, varies unit-genome structure while homogenizing the nucleotide sequence of repeats.
[Mh] Termos MeSH primário: Conversão Gênica/genética
Genomas de Plastídeos/genética
Recombinação Genética/genética
[Mh] Termos MeSH secundário: Replicação do DNA/genética
Genoma de Planta/genética
Geraniaceae/genética
Sequências Repetitivas de Ácido Nucleico/genética
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600453


  6 / 262 MEDLINE  
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[PMID]:28399123
[Au] Autor:Choi IS; Choi BH
[Ad] Endereço:Department of Biological Sciences, Inha University, Incheon, Republic of Korea.
[Ti] Título:The distinct plastid genome structure of Maackia fauriei (Fabaceae: Papilionoideae) and its systematic implications for genistoids and tribe Sophoreae.
[So] Source:PLoS One;12(4):e0173766, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traditionally, the tribe Sophoreae sensu lato has been considered a basal but also heterogeneous taxonomic group of the papilionoid legumes. Phylogenetic studies have placed Sophoreae sensu stricto (s.s.) as a member of the core genistoids. The recently suggested new circumscription of this tribe involved the removal of traditional members and the inclusion of Euchresteae and Thermopsideae. Nonetheless, definitions and inter- and intra-taxonomic issues of Sophoreae remain unclear. Within the field of legume systematics, the molecular characteristics of a plastid genome (plastome) have an important role in helping to define taxonomic groups. Here, we examined the plastome of Maackia fauriei, belonging to Sophoreae s.s., to elucidate the molecular characteristics of Sophoreae. Its gene contents are similar to the plastomes of other typical legumes. Putative pseudogene rps16 of Maackia and Lupinus species imply independent functional gene loss from the genistoids. Our overall examination of that loss among legumes suggests that it is common among all major clades of Papilionoideae. The M. fauriei plastome has a novel 24-kb inversion in its large single copy region, as well as previously recognized 50-kb and 36-kb inversions. The 36-kb inversion is shared by the core genistoids. The 24-kb inversion is present in the eight genera belonging to three tribes: Euchresteae, Sophoreae s.s., and Thermopsideae. The phylogenetic distribution of this 24-kb inversion strongly supports the monophyly of members of Sophoreae s.s. with Euchresteae and Thermopsideae. Hence, it can be used as a putative synapomorphic characteristic for the newly circumscribed Sophoreae, including Euchresteae and Thermopsideae. However, plastome conformation suggests a slightly narrower taxonomic group because of heterogeneous results from Bolusanthus and Dicraeopetalum. The phylogenetic analysis, based on plastome sequences from 43 legumes, represents well our understanding of legume systematics while resolving the genistoid clade as a sister group to an Old World clade. It also demonstrates the value that plastomes are powerful marker for systematic studies of basal papilionoid legumes.
[Mh] Termos MeSH primário: Genomas de Plastídeos
Maackia/genética
[Mh] Termos MeSH secundário: Variação Genética
Filogenia
Plastídeos/genética
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173766


  7 / 262 MEDLINE  
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[PMID]:28397003
[Au] Autor:Schwarz EN; Ruhlman TA; Weng ML; Khiyami MA; Sabir JSM; Hajarah NH; Alharbi NS; Rabah SO; Jansen RK
[Ad] Endereço:Department of Biological Sciences, St. Edward's University, Austin, TX, 78704, USA. enschwarz@utexas.edu.
[Ti] Título:Plastome-Wide Nucleotide Substitution Rates Reveal Accelerated Rates in Papilionoideae and Correlations with Genome Features Across Legume Subfamilies.
[So] Source:J Mol Evol;84(4):187-203, 2017 Apr.
[Is] ISSN:1432-1432
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study represents the most comprehensive plastome-wide comparison of nucleotide substitution rates across the three subfamilies of Fabaceae: Caesalpinioideae, Mimosoideae, and Papilionoideae. Caesalpinioid and mimosoid legumes have large, unrearranged plastomes compared with papilionoids, which exhibit varying levels of rearrangement including the loss of the inverted repeat (IR) in the IR-lacking clade (IRLC). Using 71 genes common to 39 legume taxa representing all the three subfamilies, we show that papilionoids consistently have higher nucleotide substitution rates than caesalpinioids and mimosoids, and rates in the IRLC papilionoids are generally higher than those in the IR-containing papilionoids. Unsurprisingly, this pattern was significantly correlated with growth habit as most papilionoids are herbaceous, whereas caesalpinioids and mimosoids are largely woody. Both nonsynonymous (dN) and synonymous (dS) substitution rates were also correlated with several biological features including plastome size and plastomic rearrangements such as the number of inversions and indels. In agreement with previous reports, we found that genes in the IR exhibit between three and fourfold reductions in the substitution rates relative to genes within the large single-copy or small single-copy regions. Furthermore, former IR genes in IR-lacking taxa exhibit accelerated rates compared with genes contained in the IR.
[Mh] Termos MeSH primário: Fabaceae/genética
Genomas de Plastídeos/genética
[Mh] Termos MeSH secundário: Evolução Molecular
Genoma de Planta/genética
Sequências Repetidas Invertidas/genética
Mutação
Nucleotídeos/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleotides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1007/s00239-017-9792-x


  8 / 262 MEDLINE  
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[PMID]:28383641
[Au] Autor:Sullivan AR; Schiffthaler B; Thompson SL; Street NR; Wang XR
[Ad] Endereço:Department of Ecology and Environmental Science, Umeå Plant Science Center, Umeå University, Umeå, Sweden.
[Ti] Título:Interspecific Plastome Recombination Reflects Ancient Reticulate Evolution in Picea (Pinaceae).
[So] Source:Mol Biol Evol;34(7):1689-1701, 2017 Jul 01.
[Is] ISSN:1537-1719
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plastid sequences are a cornerstone in plant systematic studies and key aspects of their evolution, such as uniparental inheritance and absent recombination, are often treated as axioms. While exceptions to these assumptions can profoundly influence evolutionary inference, detecting them can require extensive sampling, abundant sequence data, and detailed testing. Using advancements in high-throughput sequencing, we analyzed the whole plastomes of 65 accessions of Picea, a genus of ∼35 coniferous forest tree species, to test for deviations from canonical plastome evolution. Using complementary hypothesis and data-driven tests, we found evidence for chimeric plastomes generated by interspecific hybridization and recombination in the clade comprising Norway spruce (P. abies) and 10 other species. Support for interspecific recombination remained after controlling for sequence saturation, positive selection, and potential alignment artifacts. These results reconcile previous conflicting plastid-based phylogenies and strengthen the mounting evidence of reticulate evolution in Picea. Given the relatively high frequency of hybridization and biparental plastid inheritance in plants, we suggest interspecific plastome recombination may be more widespread than currently appreciated and could underlie reported cases of discordant plastid phylogenies.
[Mh] Termos MeSH primário: Transferência Genética Horizontal
Picea/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Evolução Biológica
DNA de Plantas/genética
Evolução Molecular
Genes de Plantas
Genomas de Plastídeos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Hibridização Genética
Filogenia
Plastídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1093/molbev/msx111


  9 / 262 MEDLINE  
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[PMID]:28360398
[Au] Autor:Singh N; Meena B; Pal AK; Roy RK; Tewari SK; Tamta S; Rana TS
[Ad] Endereço:CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India. ranatikam@gmail.com.
[Ti] Título:Nucleotide diversity and phylogenetic relationships among Gladiolus cultivars and related taxa of family Iridaceae.
[So] Source:J Genet;96(1):135-145, 2017 Mar.
[Is] ISSN:0973-7731
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The plastid genome regions of two intergenic spacers, psbA-trnH and trnL-trnF, were sequenced to study the nucleotide diversity and phylogenetic relationships among Gladiolus cultivars. Nucleotide diversity of psbA-trnH region was higher than trnL-trnF region of chloroplast. We employed Bayesian, maximum parsimony (MP) and neighbour-joining (NJ) approaches for phylogenetic analysis of Gladiolus and related taxa using combined datasets from chloroplast genome. The psbA-trnH and trnL-trnF intergenic spacers of Gladiolus and related taxa-like Babiana, Chasmanthe, Crocus, Iris, Moraea, Sisyrinchium, Sparaxis and two out group species (Hymenocallis littoralis and Asphodeline lutea) were used in the present investigation. Results showed that subfamily Iridoideae have sister lineage with subfamily Ixioideae and Crocoideae. H. littoralis and A. lutea were separately attached at the base of tree as the diverging Iridaceae relative's lineage. Present study revealed that psbA-trnH region are useful in addressing questions of phylogenetic relationships among the Gladiolus cultivars, as these intergenic spacers are more variable and have more phylogenetically informative sites than the trnL-trnF spacer, and therefore, are suitable for phylogenetic comparison on a lower taxonomic level. Gladiolus cultivars are extensively used as an ornamental crop and showed high potential in floriculture trade. Gladiolus cultivation still needs to generate new cultivars with stable phenotypes. Moreover, one of the most popular methods for generating new cultivars is hybridization. Hence, information on phylogenetic relationships among cultivars could be useful for hybridization programmes for further improvement of the crop.
[Mh] Termos MeSH primário: DNA de Plantas
Variação Genética
Iridaceae/classificação
Iridaceae/genética
Nucleotídeos
Filogenia
[Mh] Termos MeSH secundário: Cloroplastos/genética
DNA Espaçador Ribossômico
Evolução Molecular
Genomas de Plastídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal Spacer); 0 (Nucleotides)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


  10 / 262 MEDLINE  
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[PMID]:28330552
[Au] Autor:Zang X; Geng X; Liu K; Wang F; Liu Z; Zhang L; Zhao Y; Tian X; Hu Z; Yao Y; Ni Z; Xin M; Sun Q; Peng H
[Ad] Endereço:State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.
[Ti] Título:Ectopic expression of TaOEP16-2-5B, a wheat plastid outer envelope protein gene, enhances heat and drought stress tolerance in transgenic Arabidopsis plants.
[So] Source:Plant Sci;258:1-11, 2017 May.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Abiotic stresses, such as heat and drought, are major environmental factors restricting crop productivity and quality worldwide. A plastid outer envelope protein gene, TaOEP16-2, was identified from our previous transcriptome analysis [1,2]. In this study, the isolation and functional characterization of the TaOEP16-2 gene was reported. Three homoeologous sequences of TaOEP16-2 were isolated from hexaploid wheat, which were localized on the chromosomes 5A, 5B and 5D, respectively. These three homoeologues exhibited different expression patterns under heat stress conditions, TaOEP16-2-5B was the dominant one, and TaOEP16-2-5B was selected for further analysis. Compared with wild type (WT) plants, transgenic Arabidopsis plants overexpressing the TaOEP16-2-5B gene exhibited enhanced tolerance to heat stress, which was supported by improved survival rate, strengthened cell membrane stability and increased sucrose content. It was also found that TaOEP16-2 was induced by drought stress and involved in drought stress tolerance. TaOEP16-2-5B has the same function in ABA-controlled seed germination as AtOEP16-2. Our results suggest that TaOEP16-2-5B plays an important role in heat and drought stress tolerance, and could be utilized in transgenic breeding of wheat and other crop plants.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Desidratação/genética
Expressão Ectópica do Gene
Plantas Geneticamente Modificadas/fisiologia
Termotolerância/genética
Triticum/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/metabolismo
Clonagem Molecular
Desidratação/fisiopatologia
Expressão Ectópica do Gene/genética
Expressão Ectópica do Gene/fisiologia
Regulação da Expressão Gênica de Plantas
Genomas de Plastídeos/genética
Genomas de Plastídeos/fisiologia
Filogenia
Proteínas de Plantas/genética
Proteínas de Plantas/fisiologia
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Plastídeos/genética
Termotolerância/fisiologia
Triticum/metabolismo
Triticum/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE



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