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[PMID]:26397128
[Au] Autor:Yang Y; Zhang A; Lei C; Wang H; Guan Z; Xu C; Liu B; Zhang D; Li Q; Jiang W; Pan Y; Yang C
[Ad] Endereço:1 College of Life Science, Sichuan University , Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, China .
[Ti] Título:Characteristics of Plasmids Coharboring 16S rRNA Methylases, CTX-M, and Virulence Factors in Escherichia coli and Klebsiella pneumoniae Isolates from Chickens in China.
[So] Source:Foodborne Pathog Dis;12(11):873-80, 2015 Nov.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to characterize plasmids coharboring 16S rRNA methylases, blaCTX-M and virulence-associated genes in Escherichia coli and Klebsiella pneumoniae isolates from chickens in China. A total of 32 positive transconjugants exhibited coresistance to amikacin and cefotaxime in E. coli (24/281) and K. pneumoniae (8/93), and were identified by conjugation experiments and S1-pulsed-field gel electrophoresis. Polymerase chain reaction amplification assay detecting resistance genes showed that rmtB or armA gene accompanied with different blaCTX-M genes coexisted on 32 transferred plasmids. The blaCTX-M-98b gene was identified in chicken-derived E. coli and K. pneumoniae for the first time. The association between resistance genes and virulence genes was observed in the transferred plasmids; 68.8% (22/32) transferred resistance plasmids coharboring various virulence genes including traT, iutA, fyuA, msbB, and vagC genes with diverse proportions. Genetic stability tests revealed that 93.8% (30/32) transferred plasmids continued to exist in the host strain after continuous passage of 30 times in 15 days. Furthermore, 87.5% (28/32) conjugants showed no significant differences in growth rates compared with E. coli J53. Results of the growth competition assay showed that conjugants have low fitness cost, which indicated that there were no obvious negative effects on the host's growth. The combination of blaCTX-M-98b-rmtB-traT on 85-kb transferred IncF plasmids in E. coli, and blaCTX-M-14-rmtB-traT on 95-kb transferred IncF plasmids in K. pneumoniae were first identified in this study. These features of plasmids may contribute to the successful spread of resistance and virulence among pathogens of different sources and geographical origins.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Plasmídeos de Bacteriocinas/genética
Galinhas/microbiologia
Escherichia coli/genética
Klebsiella pneumoniae/genética
tRNA Metiltransferases/genética
[Mh] Termos MeSH secundário: Amicacina/farmacologia
Animais
Antibacterianos/farmacologia
Proteínas de Bactérias/efeitos dos fármacos
Plasmídeos de Bacteriocinas/efeitos dos fármacos
Cefotaxima/farmacologia
China
Conjugação Genética
Farmacorresistência Bacteriana/genética
Eletroforese em Gel de Campo Pulsado
Escherichia coli/enzimologia
Escherichia coli/isolamento & purificação
Escherichia coli/patogenicidade
Infecções por Escherichia coli/genética
Infecções por Escherichia coli/veterinária
Proteínas de Escherichia coli/genética
Infecções por Klebsiella/genética
Infecções por Klebsiella/veterinária
Klebsiella pneumoniae/enzimologia
Klebsiella pneumoniae/isolamento & purificação
Klebsiella pneumoniae/patogenicidade
Metiltransferases/genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/análise
RNA Ribossômico 16S/genética
Fatores de Virulência/genética
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Escherichia coli Proteins); 0 (RNA, Ribosomal, 16S); 0 (Virulence Factors); 84319SGC3C (Amikacin); EC 2.1.1.- (Methyltransferases); EC 2.1.1.- (tRNA Methyltransferases); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase CTX-M, E coli); N2GI8B1GK7 (Cefotaxime)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151107
[Lr] Data última revisão:
151107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150924
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2015.2025


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[PMID]:26055257
[Au] Autor:Miyamoto K; Seike S; Takagishi T; Okui K; Oda M; Takehara M; Nagahama M
[Ad] Endereço:Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 180, Tokushima, 770-8514, Japan. kazupitts@jtw.zaq.ne.jp.
[Ti] Título:Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603.
[So] Source:BMC Microbiol;15:118, 2015 Jun 09.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Most recent studies of Clostridium perfringens plasmids have focused on toxin-encoding or antibiotic resistance plasmids. To cause intestinal disease, a toxigenic strain must grow in the intestines to levels allowing for sufficient toxin production and this in vivo growth often involves overcoming the normal intestinal microbial population. For this purpose, bacteriocin production might be important. RESULTS: In this study, as the first step in the genetic analysis of a co-existing plasmid with an enterotoxin gene (cpe)-encoding plasmid, the bacteriocin gene-encoding plasmid, pBCNF5603, was completely sequenced. This plasmid has some homology with two previously sequenced C. perfringens plasmids, namely, pCP13 carrying a cpb2 gene and pIP404 carrying a bcn gene. Using recombinant plasmids, the rep gene homologous to the PCP63 gene on pCP13 appeared to be functional. Comparative genomics indicated that the identified rep gene homologs were found on two additional toxin plasmids, pCP-OS1 and pCP-TS1. While functional analysis using recombinant plasmids indicated that pBCNF5603 and pCP13 are likely to be incompatible, the plasmid replication and partitioning region of pBCNF5603 alone was insufficient for stable maintenance of this plasmid. CONCLUSIONS: These findings suggest that pBCNF5603 evolved from recombination events between C. perfringens plasmids and inter-species mobile genetic element(s). In addition, the bcn-encoding plasmid, pBCNF5603, is likely to be included in the Inc family, which includes pCP13 and two variant iota-encoding plasmids. Furthermore, the bcn gene on pBCNF5603 could contribute to gastrointestinal disease induced by enterotoxigenic C. perfringens.
[Mh] Termos MeSH primário: Plasmídeos de Bacteriocinas/genética
Clostridium perfringens/genética
Replicação do DNA
[Mh] Termos MeSH secundário: Enterotoxinas/genética
Enterotoxinas/metabolismo
Dados de Sequência Molecular
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enterotoxins)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150612
[Lr] Data última revisão:
150612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150610
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-015-0443-3


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[PMID]:25653018
[Au] Autor:Jackson CR; Davis JA; Frye JG; Barrett JB; Hiott LM
[Ad] Endereço:Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, Russell Research Center, Athens, GA, USA.
[Ti] Título:Diversity of Plasmids and Antimicrobial Resistance Genes in Multidrug-Resistant Escherichia coli Isolated from Healthy Companion Animals.
[So] Source:Zoonoses Public Health;62(6):479-88, 2015 Sep.
[Is] ISSN:1863-2378
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The presence and transfer of antimicrobial resistance genes from commensal bacteria in companion animals to more pathogenic bacteria may contribute to dissemination of antimicrobial resistance. The purpose of this study was to determine antimicrobial resistance gene content and the presence of genetic elements in antimicrobial resistant Escherichia coli from healthy companion animals. In our previous study, from May to August, 2007, healthy companion animals (155 dogs and 121 cats) from three veterinary clinics in the Athens, GA, USA area were sampled and multidrug-resistant E. coli (n = 36; MDR, resistance to ≥ 2 antimicrobial classes) were obtained. Of the 25 different plasmid replicon types tested by PCR, at least one plasmid replicon type was detected in 94% (34/36) of the MDR E. coli; four isolates contained as many as five different plasmid replicons. Nine replicon types (FIA, FIB, FII, I2, A/C, U, P, I1 and HI2) were identified with FIB, FII, I2 as the most common pattern. The presence of class I integrons (intI) was detected in 61% (22/36) of the isolates with eight isolates containing aminoglycoside- and/or trimethoprim-resistance genes in the variable cassette region of intI. Microarray analysis of a subset of the MDR E. coli (n = 9) identified the presence of genes conferring resistance to aminoglycosides (aac, aad, aph and strA/B), ß-lactams (ampC, cmy, tem and vim), chloramphenicol (cat), sulfonamides (sulI and sulII), tetracycline [tet(A), tet(B), tet(C), tet(D) and regulator, tetR] and trimethoprim (dfrA). Antimicrobial resistance to eight antimicrobials (ampicillin, cefoxitin, ceftiofur, amoxicillin/clavulanic acid, streptomycin, gentamicin, sulfisoxazole and trimethoprim-sulfamethoxazole) and five plasmid replicons (FIA, FIB, FII, I1 and I2) were transferred via conjugation. The presence of antimicrobial resistance genes, intI and transferable plasmid replicons indicate that E. coli from companion animals may play an important role in the dissemination of antimicrobial resistance, particularly to human hosts during contact.
[Mh] Termos MeSH primário: Plasmídeos de Bacteriocinas/farmacologia
Gatos/microbiologia
Cães/microbiologia
Farmacorresistência Bacteriana Múltipla/genética
Escherichia coli/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Animais Domésticos
Antibacterianos/farmacologia
Plasmídeos de Bacteriocinas/genética
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Genes Bacterianos/efeitos dos fármacos
Georgia
Seres Humanos
Integrons
Animais de Estimação
Plasmídeos
Reação em Cadeia da Polimerase
Replicon/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150807
[Lr] Data última revisão:
150807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150206
[St] Status:MEDLINE
[do] DOI:10.1111/zph.12178


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[PMID]:25450765
[Au] Autor:Morales M; Attai H; Troy K; Bermudes D
[Ad] Endereço:Biology Department, California State University Northridge, Northridge, CA 91330-8303, United States.
[Ti] Título:Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.
[So] Source:Plasmid;77:7-16, 2015 Jan.
[Is] ISSN:1095-9890
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We sequenced the complete 7118 bp circular plasmid pColE3-CA38 (pColE3) from Escherichia coli, located the previously identified colicin components together with two new ORFs that have homology to mobilization and transfer proteins, and found that pColE3 is highly similar to a plasmid present in enterohemorrhagic E. coli O111. We also found that unusual aspects of the plasmid include the inability to be completely digested with restriction endonucleases and asymmetric Phred DNA sequencing quality scores, with significantly lower scores in the forward direction relative to the colicin and immunity proteins consistent with plus (+) strand DNA. Comparing the A260 with picogreen double-stranded DNA (dsDNA) fluorescence and oligreen single-stranded DNA (ssDNA) fluorescence as well as metachromatic staining by acridine orange, we found that the undigested pColE3 DNA stains preferentially as ssDNA and that it coexists with dsDNA. We also identified ssDNA in pColE5 and pColE9 but not in pColE1. Colicin plasmids producing ssDNA may represent a new subclass of rolling-circle replication plasmids and add to the known similarities between colicins and filamentous phage.
[Mh] Termos MeSH primário: Plasmídeos de Bacteriocinas/genética
DNA Bacteriano/metabolismo
DNA de Cadeia Simples/metabolismo
Escherichia coli/genética
[Mh] Termos MeSH secundário: Laranja Acridina/metabolismo
Sequência de Bases
Corantes/metabolismo
Fluorescência
Dosagem de Genes
Dados de Sequência Molecular
Mapeamento por Restrição
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Coloring Agents); 0 (DNA, Bacterial); 0 (DNA, Single-Stranded); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:24896372
[Au] Autor:Borrero J; Chen Y; Dunny GM; Kaznessis YN
[Ad] Endereço:†Department of Chemical Engineering and Materials Science, ‡Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, United States.
[Ti] Título:Modified lactic acid bacteria detect and inhibit multiresistant enterococci.
[So] Source:ACS Synth Biol;4(3):299-306, 2015 Mar 20.
[Is] ISSN:2161-5063
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We designed Lactococcus lactis to detect Enterococcus faecalis. Upon detection, L. lactis produce and secrete antienterococcal peptides. The peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of L. lactis. The enterococcal sex pheromone cCF10 serves as the signal for detection. Expression vectors derived from pCF10, a cCF10-responsive E. faecalis sex-pheromone conjugative plasmid, were engineered in L. lactis for the detection system. Recombinant host strains were engineered to express genes for three bacteriocins, enterocin A, hiracin JM79 and enterocin P, each with potent antimicrobial activity against E. faecalis. Sensitive detection and specific inhibition occur both in agar and liquid media. The engineered L. lactis also inhibited growth of multidrug-resistant E. faecium strains, when induced by cCF10. The presented vectors and strains can be components of a toolbox for the development of alternative antibiotic technologies targeting enterococci at the site of infection.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Plasmídeos de Bacteriocinas/genética
Bacteriocinas/farmacologia
Técnicas Biossensoriais/métodos
Enterococcus faecalis/efeitos dos fármacos
Enterococcus faecalis/isolamento & purificação
Lactococcus lactis/genética
[Mh] Termos MeSH secundário: Antibacterianos/metabolismo
Bacteriocinas/genética
Bacteriocinas/metabolismo
Lactococcus lactis/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, AMERICAN RECOVERY AND REINVESTMENT ACT; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacteriocins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140605
[St] Status:MEDLINE
[do] DOI:10.1021/sb500090b


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[PMID]:24972810
[Au] Autor:Kang HL; Jo JS; Kwon SU; Song JY; Seo JH; Cho MJ; Baik SC; Youn HS; Rhee KH; Lee WK
[Ad] Endereço:Department of Microbiology, Gyeongsang National University School of Medicine, Jinju, 660-751, Republic of Korea.
[Ti] Título:An easy way for the rapid purification of recombinant proteins from Helicobacter pylori using a newly designed expression vector.
[So] Source:J Microbiol;52(7):604-8, 2014 Jul.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:We constructed a H. pylori expression vector which consisted of both a His-tag and a GST tag as purification tools for recombinant protein and a chloramphenicol resistant cat gene as a reporter. The backbone of the vector pBK contained an ColEI origin of replication and a kanamycin resistant gene. A set of oligos for the His-tag and the PCR product of gst (glutathione S-transferase) gene were inserted sequentially in frame in the multi-cloning site of pBK. The orf of cat was inserted downstream of the gst to generate pBKHGC. The 3' part of H. pylori clpB and flaA were cloned into the vector which was introduced into H. pylori. Recombinant proteins were purified by GSH affinity column, digested with thrombin and were analyzed by western blotting. The final recombinant proteins were successfully purified.
[Mh] Termos MeSH primário: Expressão Gênica
Vetores Genéticos
Helicobacter pylori/genética
Proteínas Recombinantes de Fusão/isolamento & purificação
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Plasmídeos de Bacteriocinas/genética
Cromatografia de Afinidade/métodos
Clonagem Molecular/métodos
Farmacorresistência Bacteriana
Helicobacter pylori/metabolismo
Canamicina/farmacologia
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Origem de Replicação
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Recombinant Fusion Proteins); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140629
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-014-3679-y


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[PMID]:23651844
[Au] Autor:McCoy DD; Zhou L; Nguyen AK; Watts AG; Donovan CM; McKemy DD
[Ad] Endereço:Neurobiology Section, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
[Ti] Título:Enhanced insulin clearance in mice lacking TRPM8 channels.
[So] Source:Am J Physiol Endocrinol Metab;305(1):E78-88, 2013 Jul 01.
[Is] ISSN:1522-1555
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blood glucose concentration is tightly regulated by the rate of insulin secretion and clearance, a process partially controlled by sensory neurons serving as metabolic sensors in relevant tissues. The activity of these neurons is regulated by the products of metabolism which regulate transmitter release, and recent evidence suggests that neuronally expressed ion channels of the transient receptor potential (TRP) family function in this critical process. Here, we report the novel finding that the cold and menthol-gated channel TRPM8 is necessary for proper insulin homeostasis. Mice lacking TRPM8 respond normally to a glucose challenge while exhibiting prolonged hypoglycemia in response to insulin. Additionally, Trpm8-/- mice have increased rates of insulin clearance compared with wild-type animals and increased expression of insulin-degrading enzyme in the liver. TRPM8 channels are not expressed in the liver, but TRPM8-expressing sensory afferents innervate the hepatic portal vein, suggesting a TRPM8-mediated neuronal control of liver insulin clearance. These results demonstrate that TRPM8 is a novel regulator of serum insulin and support the role of sensory innervation in metabolic homeostasis.
[Mh] Termos MeSH primário: Glicemia/metabolismo
Hipoglicemia/genética
Insulina/metabolismo
Células Receptoras Sensoriais/metabolismo
Canais de Cátion TRPM/genética
[Mh] Termos MeSH secundário: Animais
Plasmídeos de Bacteriocinas
Diabetes Mellitus Experimental/metabolismo
Homeostase/fisiologia
Hipoglicemia/metabolismo
Células Secretoras de Insulina/metabolismo
Fígado/irrigação sanguínea
Fígado/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Veia Porta/inervação
Ratos
Canais de Cátion TRPM/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Insulin); 0 (TRPM Cation Channels); 0 (TRPM8 protein, mouse)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130509
[St] Status:MEDLINE
[do] DOI:10.1152/ajpendo.00542.2012


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[PMID]:23127914
[Au] Autor:Han EJ; Lee NK; Choi SY; Paik HD
[Ad] Endereço:Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University, #1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
[Ti] Título:Short communication: Bacteriocin KC24 produced by Lactococcus lactis KC24 from kimchi and its antilisterial effect in UHT milk.
[So] Source:J Dairy Sci;96(1):101-4, 2013 Jan.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The severity of Listeria monocytogenes infections emphasizes the need for prevention or elimination of the pathogen from dairy products. Lactococcus lactis KC24, isolated from kimchi, exhibited an antimicrobial effect against food pathogens, including L. monocytogenes ATCC 15313. Lactococcus lactis KC24 was cultured in a 5-L jar fermenter at 35°C, and bacteriocin activity was maximal at 4 h of incubation and persisted for 20 h. Bacteriocin KC24 was inactivated by protease XIV, indicating that it has a proteinaceous nature. Bacteriocin activity was maintained at pH 3.0 to 9.0 and at temperatures of 50 to 121°C. The mode of inhibition against L. monocytogenes ATCC 15313 was shown to involve a bactericidal effect by treatment with 100 and 200 arbitrary units (AU)/mL of bacteriocin KC24. To test the activity of bacteriocin KC24 in a food product, bacteriocin KC24 and nisin (100 and 200 AU/mL) with 4 log cfu/mL of a mixed culture of L. monocytogenes (ATCC 15313, ScottA, H7962, and H7762) were applied to UHT milk. Compared with the control, treatment with bacteriocin KC24 completely inhibited the growth of L. monocytogenes and resulted in no detectable L. monocytogenes after 14 d at 4°C, whereas nisin moderately inhibited L. monocytogenes, resulting in a final concentration after 14 d at 4°C higher than the initial inoculum. Bacteriocin KC24 may prove useful in improving the safety of dairy products.
[Mh] Termos MeSH primário: Plasmídeos de Bacteriocinas/biossíntese
Lactococcus lactis/metabolismo
Listeria monocytogenes/efeitos dos fármacos
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
Fermentação
Microbiologia de Alimentos
Leite/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents)
[Em] Mês de entrada:1306
[Cu] Atualização por classe:121225
[Lr] Data última revisão:
121225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121107
[St] Status:MEDLINE


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[PMID]:22720670
[Au] Autor:Lemaître C; Bidet P; Bingen E; Bonacorsi S
[Ad] Endereço:Université Paris Diderot, Sorbonne Paris Cité, EA 3105, Paris, France.
[Ti] Título:Transcriptional analysis of the Escherichia coli ColV-Ia plasmid pS88 during growth in human serum and urine.
[So] Source:BMC Microbiol;12:115, 2012 Jun 21.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The sequenced O45:K1:H7 Escherichia coli meningitis strain S88 harbors a large virulence plasmid. To identify possible genetic determinants of pS88 virulence, we examined the transcriptomes of 88 plasmidic ORFs corresponding to known and putative virulence genes, and 35 ORFs of unknown function. RESULTS: Quantification of plasmidic transcripts was obtained by quantitative real-time reverse transcription of extracted RNA, normalized on three housekeeping genes. The transcriptome of E. coli strain S88 grown in human serum and urine ex vivo were compared to that obtained during growth in Luria Bertani broth, with and without iron depletion. We also analyzed the transcriptome of a pS88-like plasmid recovered from a neonate with urinary tract infection. The transcriptome obtained after ex vivo growth in serum and urine was very similar to those obtained in iron-depleted LB broth. Genes encoding iron acquisition systems were strongly upregulated. ShiF and ORF 123, two ORFs encoding protein with hypothetical function and physically linked to aerobactin and salmochelin loci, respectively, were also highly expressed in iron-depleted conditions and may correspond to ancillary iron acquisition genes. Four ORFs were induced ex vivo, independently of the iron concentration. Other putative virulence genes such as iss, etsC, ompTp and hlyF were not upregulated in any of the conditions studied. Transcriptome analysis of the pS88-like plasmid recovered in vivo showed a similar pattern of induction but at much higher levels. CONCLUSION: We identify new pS88 genes potentially involved in the growth of E. coli meningitis strain S88 in human serum and urine.
[Mh] Termos MeSH primário: Plasmídeos de Bacteriocinas
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Soro/microbiologia
Transcriptoma
Urina/microbiologia
[Mh] Termos MeSH secundário: Pré-Escolar
Proteínas de Escherichia coli/biossíntese
Perfilação da Expressão Gênica
Seres Humanos
Lactente
Transcrição Genética
Fatores de Virulência/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1211
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120623
[St] Status:MEDLINE
[do] DOI:10.1186/1471-2180-12-115


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[PMID]:22403614
[Au] Autor:L'Abée-Lund TM; Jørgensen HJ; O'Sullivan K; Bohlin J; Ligård G; Granum PE; Lindbäck T
[Ad] Endereço:Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Oslo, Norway.
[Ti] Título:The highly virulent 2006 Norwegian EHEC O103:H25 outbreak strain is related to the 2011 German O104:H4 outbreak strain.
[So] Source:PLoS One;7(3):e31413, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In 2006, a severe foodborne EHEC outbreak occured in Norway. Seventeen cases were recorded and the HUS frequency was 60%. The causative strain, Esherichia coli O103:H25, is considered to be particularly virulent. Sequencing of the outbreak strain revealed resemblance to the 2011 German outbreak strain E. coli O104:H4, both in genome and Shiga toxin 2-encoding (Stx2) phage sequence. The nucleotide identity between the Stx2 phages from the Norwegian and German outbreak strains was 90%. During the 2006 outbreak, stx(2)-positive O103:H25 E. coli was isolated from two patients. All the other outbreak associated isolates, including all food isolates, were stx-negative, and carried a different phage replacing the Stx2 phage. This phage was of similar size to the Stx2 phage, but had a distinctive early phage region and no stx gene. The sequence of the early region of this phage was not retrieved from the bacterial host genome, and the origin of the phage is unknown. The contaminated food most likely contained a mixture of E. coli O103:H25 cells with either one of the phages.
[Mh] Termos MeSH primário: Surtos de Doenças
Infecções por Escherichia coli/epidemiologia
Escherichia coli/classificação
Escherichia coli/patogenicidade
[Mh] Termos MeSH secundário: Plasmídeos de Bacteriocinas/genética
Bacteriófagos/genética
Clonagem Molecular
DNA Viral/genética
Escherichia coli/genética
Escherichia coli/virologia
Infecções por Escherichia coli/microbiologia
Genoma Bacteriano/genética
Alemanha/epidemiologia
Dados de Sequência Molecular
Noruega/epidemiologia
Filogenia
Toxina Shiga II/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Shiga Toxin 2)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120310
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0031413



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