Base de dados : MEDLINE
Pesquisa : G05.360.600.500 [Categoria DeCS]
Referências encontradas : 77 [refinar]
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[PMID]:9804924
[Au] Autor:Gollapudi BB; Jackson KM; Stott WT
[Ad] Endereço:The Dow Chemical, Health and Environmental Research Laboratory, 1803 Building, Midland, MI 48674, USA. bgollapudi@dow.com
[Ti] Título:Hepatic lacI and cII mutation in transgenic (lambdaLIZ) rats treated with dimethylnitrosamine.
[So] Source:Mutat Res;419(1-3):131-5, 1998 Nov 09.
[Is] ISSN:0027-5107
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The recent introduction of a transgenic rat in vivo mutation assay is a much needed supplement to the transgenic mouse models and offers the tools necessary for collecting target tissue specific genotoxicity data in this species. The utility of the Big Blue(R) rat for the detection of in vivo mutations was investigated by studying spontaneous and dimethylnitrosamine (DMN)-induced hepatic mutations. High molecular weight DNA isolated from Big Blue(R) rat livers typically yielded good transgene rescue efficiency of up to 5x105 plaque forming units per packaging reaction. DMN, when administered by oral gavage at dose levels of 0.2, 0.6, 2.0, and 6.0 mg kg-1 day-1, induced up to a 4.5-fold increase in mutations at the highest dose level. There was no apparent difference between the lacI vs. cII target genes of the shuttle vector in either the background or DMN-induced mutant frequencies. These results suggest that the transgenic rat model is a useful tool for studying potential genotoxicity in target organs and, with further validation, the selectable cII target could be an attractive alternative to the conventional lacI color screening method for the detection of mutations in the lambdaLIZ shuttle vector.
[Mh] Termos MeSH primário: Animais Geneticamente Modificados
Dimetilnitrosamina/toxicidade
Fatores de Lactose/genética
Testes de Mutagenicidade
Mutação
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Animais
Bacteriófago lambda/genética
Fígado/ultraestrutura
Ratos
Ratos Endogâmicos F344
Proteínas Virais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transcription Factors); 0 (Viral Proteins); 0 (cII protein, bacteriophage lambda); M43H21IO8R (Dimethylnitrosamine)
[Em] Mês de entrada:9812
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:981107
[St] Status:MEDLINE


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[PMID]:9192654
[Au] Autor:Smith J; Modrich P
[Ad] Endereço:Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
[Ti] Título:Removal of polymerase-produced mutant sequences from PCR products.
[So] Source:Proc Natl Acad Sci U S A;94(13):6847-50, 1997 Jun 24.
[Is] ISSN:0027-8424
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heteroduplex DNA lacking d(GATC) methylation is subject to mismatch-provoked double-strand cleavage at d(GATC) sites in a reaction dependent on MutH, MutL, MutS, and ATP. We have exploited this reaction to develop a method for removal of polymerase-produced mutant sequences that arise during sequence amplification by PCR. After denaturation and reannealing, the PCR product pool is subjected to MutH, MutL, and MutS mismatch repair proteins under double-strand cleavage conditions, followed by isolation of uncleaved product by size selection. Use of an Escherichia coli lac forward mutation assay has shown that this procedure reduces the incidence of polymerase-induced mutant sequences by an order of magnitude. Twenty mutants that originated from three independent PCR amplification reactions and survived MutHLS treatment all were found to contain an infrequently occurring A.T --> T.A transversion mutation at a unique position within the product. By contrast, the majority of mutations in untreated PCR products were transitions occurring throughout the amplified region, although frameshifts and transversions also were observed. The MutHLS method thus can be used to effectively remove the majority of mutant sequences produced by polymerase errors during PCR amplification.
[Mh] Termos MeSH primário: Mutação
Reação em Cadeia da Polimerase/métodos
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Escherichia coli/genética
Fatores de Lactose/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Em] Mês de entrada:9707
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:970624
[St] Status:MEDLINE


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[PMID]:9153652
[Au] Autor:Barkats M; Nakao N; Grasbon-Frodl EM; Bilang-Bleuel A; Revah F; Mallet J; Brundin P
[Ad] Endereço:Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, UMR CNRS C9923, Hôpital de la Pitié Salpêtrière, Paris, France.
[Ti] Título:Intrastriatal grafts of embryonic mesencephalic rat neurons genetically modified using an adenovirus encoding human Cu/Zn superoxide dismutase.
[So] Source:Neuroscience;78(3):703-13, 1997 Jun.
[Is] ISSN:0306-4522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intrastriatal grafting of embryonic dopamine-containing neurons is a promising approach for treating clinical and experimental Parkinson's disease. However, neuropathological analyses of grafted patients and transplanted rats have demonstrated that the survival of grafted dopamine neurons is relatively poor. In the present study, we pursued a strategy of transferring a potentially neuroprotective gene into rat embryonic mesencephalic rat cells in vitro, before grafting them into the denervated striatum of 6-hydroxydopamine-lesioned rats. We performed intrastriatal grafts of embryonic day 14 mesencephalic cells infected with replication-defective adenoviruses bearing either the human copper-zinc superoxide dismutase gene or, as a control, the E. coli lac Z marker gene. The transgenes were expressed in the grafts four days after transplantation and the expression persisted for at least five weeks thereafter. After five weeks postgrafting, there was more extensive functional recovery in the superoxide dismutase group as compared to the control (uninfected cells) and beta-galactosidase groups. The functional recovery was significantly correlated with the number of tyrosine hydroxylase-positive cells in the grafts, although the clear trend to increased survival of the dopamine neurons in the superoxide dismutase grafts did not reach statistical significance. Only a moderate inflammatory reaction was revealed by OX-42 immunostaining in all groups, suggesting that ex vivo gene transfer using adenoviral vectors is a promising method for delivering functional proteins into brain grafts.
[Mh] Termos MeSH primário: Transplante de Tecido Encefálico/fisiologia
Transplante de Células/fisiologia
Transplante de Tecido Fetal/fisiologia
Neostriado/transplante
Neurônios/metabolismo
Neurônios/fisiologia
Superóxido Dismutase/metabolismo
[Mh] Termos MeSH secundário: Animais
Vírus do Sarcoma Aviário/genética
Dopamina/fisiologia
Feminino
Vetores Genéticos
Sobrevivência de Enxerto
Seres Humanos
Imuno-Histoquímica
Fatores de Lactose/genética
Ratos
Ratos Sprague-Dawley
Tirosina 3-Mono-Oxigenase/metabolismo
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 1.14.16.2 (Tyrosine 3-Monooxygenase); EC 1.15.1.1 (Superoxide Dismutase); EC 3.2.1.23 (beta-Galactosidase); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:9707
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:970601
[St] Status:MEDLINE


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[PMID]:8828926
[Au] Autor:Merchant K; Chen H; Gonzalez TC; Keefer LK; Shaw BR
[Ad] Endereço:P. M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708-0346, USA.
[Ti] Título:Deamination of single-stranded DNA cytosine residues in aerobic nitric oxide solution at micromolar total NO exposures.
[So] Source:Chem Res Toxicol;9(5):891-6, 1996 Jul-Aug.
[Is] ISSN:0893-228X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deamination of cytosine to uracil is a potential source of mutations in DNA. Here we examine the deaminating ability of aerobic nitric oxide (NO) toward single-stranded DNA at very low (micromolar and below) total exposures, using a sensitive genetic method that allows us to study a single deamination event at a specific site in a 7200-nucleotide DNA molecule within a pool of ca. 100,000 other identical DNA molecules. We incubated gapped C141 M13mp2 DNA with the NO-generating compound, Et2N[N(O)NO]Na (DEA/NO), in aerobic buffer for 16 h to ensure complete autoxidation at pH 7.4 and 37 degrees C. After ultrafiltration to remove small molecules, the DNA was transformed into isogenic Escherichia coli cultures that were either deficient (NR9404, ung-) or proficient (MC1061, ung+) in uracil-DNA glycosylase activity. The gapped DNA was constructed such that the target (CCC) codon was contained in a short single-stranded segment of otherwise double-stranded circular DNA, and the incubation was performed in a closed system to prevent loss of NO to the atmosphere before the reaction was complete. An increase in the reversion frequency in the ung- strain was noted between 0 and 1 microM DEA/NO, and the reversion frequency leveled out between 3 and 30 microM. However, 30 microM "spent" DEA/NO (i.e., that which was similarly incubated for 4 h to complete the autoxidation of NO before the DNA was added) did not increase reversion frequency relative to control. Nearly all (42/43) of the mutations identified after 1 microM DEA/NO treatment were C-->T transitions, and reversion frequency in the isogenic ung+ strain was lower than in the ung- strain. The data are consistent with the hypothesis that total NO exposures in the mumol/L range can lead to C-->T mutations via a mechanism most probably involving deamination of DNA cytosine residues.
[Mh] Termos MeSH primário: Citosina/química
DNA de Cadeia Simples/química
Óxido Nítrico/química
[Mh] Termos MeSH secundário: Aerobiose
DNA de Cadeia Simples/genética
Desaminação
Escherichia coli/genética
Genes Bacterianos/genética
Fatores de Lactose
Testes de Mutagenicidade
Plasmídeos
Soluções
Uracila/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (DNA, Single-Stranded); 0 (Solutions); 31C4KY9ESH (Nitric Oxide); 56HH86ZVCT (Uracil); 8J337D1HZY (Cytosine)
[Em] Mês de entrada:9612
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:960701
[St] Status:MEDLINE


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[PMID]:8731225
[Au] Autor:Treloar H; Walters E; Margolis F; Key B
[Ad] Endereço:Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Vic, Australia.
[Ti] Título:Olfactory glomeruli are innervated by more than one distinct subset of primary sensory olfactory neurons in mice.
[So] Source:J Comp Neurol;367(4):550-62, 1996 Apr 15.
[Is] ISSN:0021-9967
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rodent olfactory epithelium consists of a mosaic of primary sensory olfactory neurons (PONs) which express distinct putative olfactory receptor proteins. Recent evidence suggests that individual subsets of these sensory neurons project to separate glomeruli in the olfactory bulb (Vassar et al., [1994] Cell 79:981-991). In the present study we have identified two distinct subsets of primary sensory olfactory neurons (PONs) in the H-OMP-LacZ-6 transgenic mouse. In these transgenic mice, a LacZ reporter gene under the control of a 294 base pair element from the 5' promoter region of the olfactory marker protein (OMP) gene was expressed in a subset of PONs located in a discrete band of neuroepithelium in the nasal cavity. These LacZ positive neurons were not randomly located within this band but were more concentrated within a locus between endoturbinates IIb and III. The axons of these neurons densely innervated three adjacent and bilaterally symmetrical glomeruli present in the ventromedial olfactory bulb. Labeling of tissue sections with the plant lectin Dolichos biflorus (DBA) revealed an independent subset of PONs in the transgenic mice. These neurons were present in a wide region of the nasal cavity that included the neuroepithelial band containing the LacZ expressing neurons. The DBA labeled axons terminated in glomeruli in the rostromedial and dorsolateral olfactory bulb surfaces. Although the glomeruli innervated by the LacZ and DBA positive axons were predominantly non-overlapping there were glomeruli in the ventral olfactory bulb that were labeled by both DBA and LacZ markers. Eight different types of glomeruli were characterized. Most notably, glomeruli were identified which were innervated partially by both or by either subset alone. In these cases, axon subsets were observed to terminate within discrete subregions of a glomerulus. These results support the hypothesis that phenotypically distinct subsets of PONs converge on to the same glomeruli but also indicate that some glomeruli are innervated by more than one subset of sensory neuron. These findings have implications for understanding how the olfactory projection is formed and how olfactory information is processed.
[Mh] Termos MeSH primário: Neurônios Aferentes/fisiologia
Mucosa Olfatória/inervação
Lectinas de Plantas
Olfato/fisiologia
[Mh] Termos MeSH secundário: Animais
Axônios/metabolismo
Axônios/ultraestrutura
Contagem de Células
Epitélio/inervação
Epitélio/fisiologia
Histocitoquímica
Fatores de Lactose
Lectinas
Camundongos
Camundongos Transgênicos
Proteínas do Tecido Nervoso/biossíntese
Proteínas do Tecido Nervoso/genética
Proteínas do Tecido Nervoso/metabolismo
Neurônios Aferentes/metabolismo
Neurônios Aferentes/ultraestrutura
Bulbo Olfatório/metabolismo
Proteína de Marcador Olfatório
Mucosa Olfatória/metabolismo
Mucosa Olfatória/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lectins); 0 (Nerve Tissue Proteins); 0 (Olfactory Marker Protein); 0 (Omp protein, mouse); 0 (Plant Lectins); 0 (dolichos biflorus agglutinin)
[Em] Mês de entrada:9610
[Cu] Atualização por classe:060317
[Lr] Data última revisão:
060317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:960415
[St] Status:MEDLINE


  6 / 77 MEDLINE  
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[PMID]:7698675
[Au] Autor:Vaughan EE; de Vos WM
[Ad] Endereço:Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.
[Ti] Título:Identification and characterization of the insertion element IS1070 from Leuconostoc lactis NZ6009.
[So] Source:Gene;155(1):95-100, 1995 Mar 21.
[Is] ISSN:0378-1119
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel insertion sequence, designated IS1070, was identified on the lactose plasmid of Leuconostoc lactis NZ6009 by nucleotide sequence analysis. The 1027-bp sequence contains partially matched (24 of 28 bp) inverted repeats and has one long open reading frame. The deduced 305-amino-acid sequence demonstrated homology to transposases of IS30 from Escherichia coli, IS4351 from Bacteroides fragilis, IS1086 from Alcaligenes eutrophus, IS1161 from Streptococcus salivarius, ISAS2 from Aeromonas salmonicida and a putative protein encoded by ORF3 of virus SpV1-R8A2 B from Spiroplasma citri. At least fifteen IS1070-like sequences were detected in the genome of the parent Lc. lactis strain and five of these were situated on plasmids. Analysis of the direct repeats of two of these copies with that of IS1070 revealed differences in the target duplication lengths.
[Mh] Termos MeSH primário: Elementos de DNA Transponíveis
DNA Bacteriano/química
Fatores de Lactose/genética
Leuconostoc/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Dados de Sequência Molecular
Fases de Leitura Aberta
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial)
[Em] Mês de entrada:9505
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:950321
[St] Status:MEDLINE


  7 / 77 MEDLINE  
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[PMID]:8043650
[Au] Autor:Gincel E; Lancelot G; Maurizot JC; Thuong NT; Vovelle F
[Ad] Endereço:CBM-CNRS, Orléans, France.
[Ti] Título:Comparison of solution structure of free and complexed lac operator by molecular modelling with NMR constraints.
[So] Source:Biochimie;76(2):141-51, 1994.
[Is] ISSN:0300-9084
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator adopt both a right-handed B helical conformation, but a more detailed analysis of the conformational parameters using the Curves program shows striking differences in the groove geometries, the rises, the twists and the total bending.
[Mh] Termos MeSH primário: Óperon Lac
Fatores de Lactose/química
Proteínas Repressoras/química
[Mh] Termos MeSH secundário: Sequência de Bases
Escherichia coli/genética
Espectroscopia de Ressonância Magnética
Modelos Moleculares
Dados de Sequência Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Repressor Proteins)
[Em] Mês de entrada:9409
[Cu] Atualização por classe:060317
[Lr] Data última revisão:
060317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:940101
[St] Status:MEDLINE


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[PMID]:8338633
[Au] Autor:Schulz WA; Ebling B; Hasse A; Zenke F; Breunig K
[Ad] Endereço:Institut für Physiologische Chemie I, Heinrich-Heine-Universität, Düsseldorf.
[Ti] Título:Highly efficient transactivation by the yeast Kluyveromyces lactis transcription factor LAC9 and its inhibition by the negative regulator GAL80 in mammalian cells.
[So] Source:Biol Chem Hoppe Seyler;374(5):313-8, 1993 May.
[Is] ISSN:0177-3593
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Expression of the LAC9 gene from the yeast Kluyveromyces lactis in HepG2 human hepatoblastoma cells efficiently induced luciferase expression from reporter plasmids containing the four LAC9 binding sites from the K. lactis GAL1-GAL10 gene linked to a basal promoter. Induction was approximately 100fold and was dependent on the presence of the UAS sequence and an intact reading frame in the LAC9 gene. Additional cotransfection of constructs expressing the K. lactis GAL80 gene reduced luciferase activity by up to 98%. This inhibition was not affected by addition of 14mM galactose to the medium. No further yeast-specific factors appear necessary for efficient inhibition of LAC9 by GAL80, but additional gene products may be required for activation by galactose.
[Mh] Termos MeSH primário: Proteínas Fúngicas/farmacologia
Kluyveromyces/genética
Fatores de Lactose/metabolismo
Proteínas Repressoras
Proteínas de Saccharomyces cerevisiae
Fatores de Transcrição/metabolismo
Ativação Transcricional
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Células Cultivadas
Regulação Fúngica da Expressão Gênica
Seres Humanos
Kluyveromyces/enzimologia
Neoplasias Hepáticas Experimentais/metabolismo
Luciferases/genética
Luciferases/metabolismo
Dados de Sequência Molecular
Plasmídeos
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (GAL80 protein, S cerevisiae); 0 (Repressor Proteins); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factors); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:9309
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:930501
[St] Status:MEDLINE


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[PMID]:8097556
[Au] Autor:Breul A; Assmann H; Golz R; von Wilcken-Bergmann B; Müller-Hill B
[Ad] Endereço:Institut für Genetik, Universität zu Köln, FRG.
[Ti] Título:Mutants with substitutions for Glu171 in the catabolite activator protein (CAP) of Escherichia coli activate transcription from the lac promoter.
[So] Source:Mol Gen Genet;238(1-2):155-60, 1993 Apr.
[Is] ISSN:0026-8925
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Single amino acid substitutions for residue Glu171 in helix E of the catabolite gene activator protein (CAP) of Escherichia coli have been reported to abolish activation of transcription without impairing binding to the CAP site of the lac promoter. The negative charge of Glu171 was proposed to transmit the activating signal from CAP to RNA polymerase. However, this idea has been challenged by later work. We set up a system to re-examine this issue. We analysed the ability of mutant CAP-E171L and CAP-E171K proteins to bind a near-consensus CAP site in vivo and found it to be diminished fourfold relative to wild type in each case. Activation of lac transcription by these mutant proteins remains the same as with wild-type CAP. Thus our results confirm that Glu171 in helix E of CAP is not involved directly in the activation of transcription. Yet CAP-E171K does not activate transcription as well as wild-type CAP under all circumstances. Possible reasons for this absence of activation are discussed.
[Mh] Termos MeSH primário: Proteína Receptora de AMP Cíclico/genética
Proteína Receptora de AMP Cíclico/metabolismo
Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Glutamatos
Fatores de Lactose/genética
Regiões Promotoras Genéticas
Transcrição Genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Sítios de Ligação
Proteína Receptora de AMP Cíclico/química
RNA Polimerases Dirigidas por DNA/metabolismo
Ácido Glutâmico
Dados de Sequência Molecular
Mutagênese Sítio-Dirigida
Plasmídeos
Reação em Cadeia da Polimerase/métodos
Estrutura Secundária de Proteína
Proteínas Recombinantes de Fusão/metabolismo
beta-Galactosidase/genética
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Gs] Símbolo de gene:lac; lacZ
[Nm] Nome de substância:
0 (Cyclic AMP Receptor Protein); 0 (Glutamates); 0 (Recombinant Fusion Proteins); 3KX376GY7L (Glutamic Acid); EC 2.7.7.6 (DNA-Directed RNA Polymerases); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:9305
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:930401
[St] Status:MEDLINE


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[PMID]:1453959
[Au] Autor:Gasson MJ; Swindell S; Maeda S; Dodd HM
[Ad] Endereço:AFRC Institute of Food Research, Norwich, UK.
[Ti] Título:Molecular rearrangement of lactose plasmid DNA associated with high-frequency transfer and cell aggregation in Lactococcus lactis 712.
[So] Source:Mol Microbiol;6(21):3213-23, 1992 Nov.
[Is] ISSN:0950-382X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:High-frequency conjugation of the lactose plasmid pLP712 is associated with a constitutive cell aggregation phenotype and is facilitated by cointegration with a sex factor. Analysis of 23 independently derived enlarged lactose plasmids revealed that the sex factor DNA present in cointegrates varied in size. This suggested that more than simple cointegration with a sex factor plasmid was involved. Further analysis led to the discovery of a chromosomally located sex factor that could excise and be lost or exist as labile plasmid DNA. Cointegration with this sex factor was shown to be promoted by transposition of a copy of ISSI present on the lactose plasmid, and models are presented to account for the complex and variable structures of the resulting enlarged lactose plasmids.
[Mh] Termos MeSH primário: Conjugação Genética/genética
DNA Bacteriano/genética
Fator F/genética
Lactococcus lactis/genética
Fatores de Lactose/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Rearranjo Gênico/genética
Dados de Sequência Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:9301
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:921101
[St] Status:MEDLINE



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde