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[PMID]:28449669
[Au] Autor:Gleicher N; Metzger J; Croft G; Kushnir VA; Albertini DF; Barad DH
[Ad] Endereço:The Center for Human Reproduction, 21 East 69th Street, New York, NY, 10021, USA. ngleicher@thechr.com.
[Ti] Título:A single trophectoderm biopsy at blastocyst stage is mathematically unable to determine embryo ploidy accurately enough for clinical use.
[So] Source:Reprod Biol Endocrinol;15(1):33, 2017 Apr 27.
[Is] ISSN:1477-7827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: It has become increasingly apparent that the trophectoderm (TE) at blastocyst stage is much more mosaic than has been appreciated. Whether preimplantation genetic screening (PGS), utilizing a single TE biopsy (TEB), can reliably determine embryo ploidy has, therefore, increasingly been questioned in parallel. METHODS: We for that reason here established 2 mathematical models to assess probabilities of false-negative and false-positive results of an on average 6-cell biopsy from an approximately 300-cell TE. This study was a collaborative effort between investigators at The Center for Human Reproduction in New York City and the Center for Studies in Physics and Biology and the Brivanlou Laboratory of Stem Cell Biology and Molecular Embryology, the latter two both at Rockefeller University in New York City. RESULTS: Both models revealed that even under best case scenario, assuming even distribution of mosaicism in TE (since mosaicism is usually clonal, a highly unlikely scenario), a biopsy of at least 27 TE cells would be required to reach minimal diagnostic predictability from a single TEB. CONCLUSIONS: As currently performed, a single TEB is, therefore, mathematically incapable of reliably determining whether an embryo can be transferred or should be discarded. Since a single TEB, as currently performed, apparently is not representative of the complete TE, this study, thus, raises additional concern about the clinical utilization of PGS.
[Mh] Termos MeSH primário: Blastocisto
Fase de Clivagem do Zigoto
Ectoderma/patologia
Ploidias
Diagnóstico Pré-Implantação/métodos
Trofoblastos/patologia
[Mh] Termos MeSH secundário: Aneuploidia
Biópsia
Blastocisto/metabolismo
Blastocisto/patologia
Fase de Clivagem do Zigoto/metabolismo
Fase de Clivagem do Zigoto/patologia
Implantação do Embrião/genética
Feminino
Seres Humanos
Modelos Teóricos
Gravidez
Diagnóstico Pré-Implantação/normas
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s12958-017-0251-8


  2 / 8971 MEDLINE  
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[PMID]:28462821
[Au] Autor:de Witte SFH; Lambert EE; Merino A; Strini T; Douben HJCW; O'Flynn L; Elliman SJ; de Klein AJEMM; Newsome PN; Baan CC; Hoogduijn MJ
[Ad] Endereço:Nephrology and Transplantation, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands. Electronic address: s.dewitte@erasmusmc.nl.
[Ti] Título:Aging of bone marrow- and umbilical cord-derived mesenchymal stromal cells during expansion.
[So] Source:Cytotherapy;19(7):798-807, 2017 07.
[Is] ISSN:1477-2566
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) are used as experimental immunotherapy. Extensive culture expansion is necessary to obtain clinically relevant cell numbers, although the impact on MSCs stability and function is unclear. This study investigated the effects of long-term in vitro expansion on the stability and function of MSCs. METHODS: Human bone marrow-derived (bmMSCs) and umbilical cord-derived (ucMSCs) MSCs were in vitro expanded. During expansion, their proliferative capacity was examined. At passages 4, 8 and 12, analyses were performed to investigate the ploidy, metabolic stability, telomere length and immunophenotype. In addition, their potential to suppress lymphocyte proliferation and susceptibility to natural killer cell lysis was examined. RESULTS: BmMSCs and ucMSCs showed decreasing proliferative capacity over time, while their telomere lengths and mitochondrial activity remained stable. Percentage of aneuploidy in cultures was unchanged after expansion. Furthermore, expression of MSC markers and markers associated with stress or aging remained unchanged. Reduced capacity to suppress CD4 and CD8 T-cell proliferation was observed for passage 8 and 12 bmMSCs and ucMSCs. Finally, susceptibility of bmMSCs and ucMSCs to NK-cell lysis remained stable. CONCLUSIONS: We showed that after long-term expansion, phenotype of bmMSCs and ucMSCs remains stable and cells exhibit similar immunogenic properties compared with lower passage cells. However, immunosuppressive properties of MSCs are reduced. These findings reveal the consequences of application of higher passage MSCs in the clinic, which will help increase the yield of therapeutic MSCs but may interfere with their efficacy.
[Mh] Termos MeSH primário: Células da Medula Óssea/citologia
Células Mesenquimais Estromais/fisiologia
Cordão Umbilical/citologia
[Mh] Termos MeSH secundário: Linfócitos T CD4-Positivos/citologia
Linfócitos T CD4-Positivos/fisiologia
Linfócitos T CD8-Positivos/citologia
Linfócitos T CD8-Positivos/fisiologia
Diferenciação Celular/efeitos dos fármacos
Proliferação Celular
Células Cultivadas
Feminino
Seres Humanos
Imunofenotipagem
Células Matadoras Naturais/imunologia
Células Matadoras Naturais/fisiologia
Células Mesenquimais Estromais/citologia
Ploidias
Gravidez
Homeostase do Telômero
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28452089
[Au] Autor:Waples RK; Seeb JE; Seeb LW
[Ad] Endereço:School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, USA.
[Ti] Título:Congruent population structure across paralogous and nonparalogous loci in Salish Sea chum salmon (Oncorhynchus keta).
[So] Source:Mol Ecol;26(16):4131-4144, 2017 Aug.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Whole-genome duplications are major evolutionary events with a lasting impact on genome structure. Duplication events complicate genetic analyses as paralogous sequences are difficult to distinguish; consequently, paralogs are often excluded from studies. The effects of an ancient whole-genome duplication (approximately 88 MYA) are still evident in salmonids through the persistence of numerous paralogous gene sequences and partial tetrasomic inheritance. We use restriction site-associated DNA sequencing on 10 collections of chum salmon from the Salish Sea in the USA and Canada to investigate genetic diversity and population structure in both tetrasomic and rediploidized regions of the genome. We use a pedigree and high-density linkage map to identify paralogous loci and to investigate genetic variation across the genome. By applying multivariate statistical methods, we show that it is possible to characterize paralogous loci and that they display similar patterns of population structure as the diploidized portion of the genome. We find genetic associations with the adaptively important trait of run-timing in both sets of loci. By including paralogous loci in genome scans, we can observe evolutionary signals in genomic regions that have routinely been excluded from population genetic studies in other polyploid-derived species.
[Mh] Termos MeSH primário: Duplicação Gênica
Variação Genética
Genética Populacional
Oncorhynchus keta/genética
[Mh] Termos MeSH secundário: Animais
Canadá
Mapeamento Cromossômico
Ligação Genética
Genoma
Linhagem
Ploidias
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/mec.14163


  4 / 8971 MEDLINE  
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[PMID]:29101997
[Au] Autor:Zaninovic N; Irani M; Meseguer M
[Ad] Endereço:The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, New York, New York. Electronic address: nizanin@med.cornell.edu.
[Ti] Título:Assessment of embryo morphology and developmental dynamics by time-lapse microscopy: is there a relation to implantation and ploidy?
[So] Source:Fertil Steril;108(5):722-729, 2017 Nov.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Time-lapse microscopy (TLM) is an exciting novel technology with great potential for enhancing embryo selection in the embryology laboratory. This non-invasive objective assessment of embryos has provided a new tool for predicting embryo development and implantation potential. TLM detects several morphological phenomena that are often missed with static observations using conventional incubators, such as irregular divisions, blastocyst collapse and re-expansion, timing of blastocoel appearance, and timing of formation and internalization of fragments. Nevertheless, it should be recognized that conventional morphological assessment has been widely accepted as the gold standard by most embryologists. TLM can enhance conventional morphological assessments to improve embryo selection and subsequent reproductive outcomes. Furthermore, morphokinetic parameters can aid in differentiating between euploid and aneuploid embryos, although they are not sufficiently accurate to replace preimplantation genetic testing for aneuploidy. Morphokinetic assessment together with chromosomal screening may ultimately help identify euploid embryos with the highest developmental potential.
[Mh] Termos MeSH primário: Blastocisto/patologia
Implantação do Embrião
Fertilização In Vitro
Infertilidade/terapia
Microscopia
Ploidias
Transferência de Embrião Único/métodos
Imagem com Lapso de Tempo
[Mh] Termos MeSH secundário: Blastocisto/metabolismo
Sobrevivência Celular
Feminino
Fertilidade
Fertilização In Vitro/efeitos adversos
Marcadores Genéticos
Seres Humanos
Infertilidade/diagnóstico
Infertilidade/fisiopatologia
Valor Preditivo dos Testes
Gravidez
Resultado da Gravidez
Transferência de Embrião Único/efeitos adversos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171106
[St] Status:MEDLINE


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[PMID]:29049296
[Au] Autor:Zhao Z; Li Y; Liu H; Zhai X; Deng M; Dong Y; Fan G
[Ad] Endereço:Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan, China.
[Ti] Título:Genome-wide expression analysis of salt-stressed diploid and autotetraploid Paulownia tomentosa.
[So] Source:PLoS One;12(10):e0185455, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Paulownia tomentosa is a fast-growing tree species with multiple uses. It is grown worldwide, but is native to China, where it is widely cultivated in saline regions. We previously confirmed that autotetraploid P. tomentosa plants are more stress-tolerant than the diploid plants. However, the molecular mechanism underlying P. tomentosa salinity tolerance has not been fully characterized. Using the complete Paulownia fortunei genome as a reference, we applied next-generation RNA-sequencing technology to analyze the effects of salt stress on diploid and autotetraploid P. tomentosa plants. We generated 175 million clean reads and identified 15,873 differentially expressed genes (DEGs) from four P. tomentosa libraries (two diploid and two autotetraploid). Functional annotations of the differentially expressed genes using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that plant hormone signal transduction and photosynthetic activities are vital for plant responses to high-salt conditions. We also identified several transcription factors, including members of the AP2/EREBP, bHLH, MYB, and NAC families. Quantitative real-time PCR analysis validated the expression patterns of eight differentially expressed genes. Our findings and the generated transcriptome data may help to accelerate the genetic improvement of cultivated P. tomentosa and other plant species for enhanced growth in saline soils.
[Mh] Termos MeSH primário: Lamiales/genética
Ploidias
Tolerância a Sal
[Mh] Termos MeSH secundário: Processamento Alternativo
Perfilação da Expressão Gênica
Genes de Plantas
Lamiales/fisiologia
Fotossíntese/genética
Reguladores de Crescimento de Planta/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Transdução de Sinais
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Transcription Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185455


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[PMID]:29025593
[Au] Autor:Seol CA; Cho YU; Jang S; Park CJ; Lee JH; Lee JH; Lee KH; Seo EJ
[Ad] Endereço:Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
[Ti] Título:Prognostic significance of recurrent additional chromosomal abnormalities in adult patients with Philadelphia chromosome-positive acute lymphoblastic leukemia.
[So] Source:Cancer Genet;216-217:29-36, 2017 Oct.
[Is] ISSN:2210-7762
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Philadelphia (Ph) chromosome-positive acute lymphoblastic leukemia (ALL), additional chromosomal abnormalities (ACAs) are frequently observed. We investigated the cytogenetic characteristics and prognostic significance of ACAs in Ph-positive ALL. We reviewed the clinical data and bone marrow cytogenetic findings of 122 adult Ph-positive ALL patients. The ACAs were examined for partial or whole chromosomal gains or losses, and structural aberrations. The overall survival (OS) and disease-free survival (DFS) of patients who received hematopoietic cell transplantation were compared between the isolated Ph group and ACA group. ACAs were present in 73.0% of all patients. The recurrent ACAs were extra Ph (24.7%), 9/9p loss (20.2%), and 7/7p loss (19.1%). Complex karyotype was found in 28.1% of patients in the ACA group. Younger patients (19-30 years) in the ACA group showed the highest frequency of extra Ph (54%) compared to other age groups. The OS in the ACA group was significantly shorter than in the isolated Ph group. The presence of an extra Ph chromosome or 9/9p loss was significantly associated with shorter OS and DFS, whereas 7/7p loss and complex karyotype were not associated with poorer prognosis. We suggest that subclassification of ACAs could be applied to prognostic investigation of Ph-positive ALL.
[Mh] Termos MeSH primário: Aberrações Cromossômicas
Cromossomo Filadélfia
Leucemia-Linfoma Linfoblástico de Células Precursoras/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Análise Citogenética
Intervalo Livre de Doença
Feminino
Transplante de Células-Tronco Hematopoéticas
Seres Humanos
Masculino
Meia-Idade
Análise Multivariada
Ploidias
Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia
Prognóstico
Modelos de Riscos Proporcionais
Recidiva
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:29025588
[Au] Autor:Do CH; Lower KM; Macardle C; Kuss BJ
[Ad] Endereço:College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
[Ti] Título:Trisomy 12 assessment by conventional fluorescence in-situ hybridization (FISH), FISH in suspension (FISH-IS) and laser scanning cytometry (LSC) in chronic lymphocytic leukemia.
[So] Source:Cancer Genet;216-217:142-149, 2017 Oct.
[Is] ISSN:2210-7762
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic lymphocytic leukemia (CLL) has an extremely heterogeneous clinical course, and prognostication is based on common genetic abnormalities which are detected by standard cytogenetic methods. However, current methods are restricted by the low number of cells able to be analyzed, resulting in the potential to miss clinically relevant sub-clonal populations of cells. A novel high throughput methodology called fluorescence in situ hybridization in suspension (FISH-IS) incorporates a flow cytometry-based imaging approach with automated analysis of thousands of cells. Here we have demonstrated that the FISH-IS technique is applicable to aneuploidy detection in CLL samples for a range of chromosomes using appropriate centromere probes. This method is able to accurately differentiate between monosomy, disomy and trisomy with a sensitivity of 1% in CLL. An analysis comparing conventional FISH, FISH-IS and laser scanning cytometry (LSC) is presented.
[Mh] Termos MeSH primário: Hibridização in Situ Fluorescente/métodos
Citometria de Varredura a Laser/métodos
Leucemia Linfocítica Crônica de Células B/diagnóstico
Leucemia Linfocítica Crônica de Células B/genética
Trissomia/genética
[Mh] Termos MeSH secundário: Cromossomos Humanos/genética
Seres Humanos
Ploidias
Cromossomos Sexuais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:28987789
[Au] Autor:La Marca A; Minasi MG; Sighinolfi G; Greco P; Argento C; Grisendi V; Fiorentino F; Greco E
[Ad] Endereço:Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, Modena, Italy; Clinica Eugin, Modena, Italy. Electronic address: antonio.lamarca@unimore.it.
[Ti] Título:Female age, serum antimüllerian hormone level, and number of oocytes affect the rate and number of euploid blastocysts in in vitro fertilization/intracytoplasmic sperm injection cycles.
[So] Source:Fertil Steril;108(5):777-783.e2, 2017 Nov.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To study the relative role of female age and ovarian reserve, measured through serum antimüllerian hormone (AMH) in determining the rate and number of euploid blastocysts in in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles. DESIGN: Retrospective analysis of cycles performed in 2014-2015. SETTING: Tertiary referral IVF center. PATIENT(S): A total of 578 infertile couples undergoing IVF/ICSI and preimplantation genetic screening (PGS) analysis. INTERVENTIONS(S): All embryos were cultured and biopsied at the blastocyst stage. The method involved whole-genome amplification followed by array comparative genome hybridization. Serum AMH was measured by means of the modified Beckman Coulter AMH Gen II assay. MAIN OUTCOME MEASURES: The rate and number of euploid blastocysts and their correlation with ovarian reserve and response to stimulation. RESULT(S): The mean (±SD) age of patients was 37.6 ± 4.1 years, and the mean number of blastocysts per patient was 3.1 ± 2. The total number of blastocysts available to the analysis was 1,814, and 36% of them were euploid after PGS. Age and serum AMH were significantly and independently related to the rate of euploid blastocysts available for patients. As an effect of the cohort size, the number of mature oocytes positively affected the total number of euploid blastocysts per patient. CONCLUSION(S): A strong positive age-independent relationship between AMH level and the rate of euploid blastocysts was found. This confirms that the measurement of ovarian reserve by means of AMH has high relevance when counseling infertile patients.
[Mh] Termos MeSH primário: Hormônio Antimülleriano/sangue
Blastocisto/patologia
Fertilização In Vitro
Infertilidade/terapia
Oócitos
Reserva Ovariana
Ovário/fisiopatologia
Ploidias
Injeções de Esperma Intracitoplásmicas
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/sangue
Biópsia
Hibridização Genômica Comparativa
Técnicas de Cultura Embrionária
Feminino
Fertilidade
Fertilização In Vitro/efeitos adversos
Seres Humanos
Infertilidade/sangue
Infertilidade/diagnóstico
Infertilidade/fisiopatologia
Masculino
Idade Materna
Ovário/metabolismo
Diagnóstico Pré-Implantação/métodos
Estudos Retrospectivos
Fatores de Risco
Injeções de Esperma Intracitoplásmicas/efeitos adversos
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 80497-65-0 (Anti-Mullerian Hormone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  9 / 8971 MEDLINE  
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[PMID]:28957348
[Au] Autor:Clawson GA; Matters GL; Xin P; McGovern C; Wafula E; dePamphilis C; Meckley M; Wong J; Stewart L; D'Jamoos C; Altman N; Imamura Kawasawa Y; Du Z; Honaas L; Abraham T
[Ad] Endereço:Gittlen Cancer Research Laboratories and the Department of Pathology, Hershey Medical Center (HMC), Pennsylvania State University (PSU), Hershey, PA, United States of America.
[Ti] Título:"Stealth dissemination" of macrophage-tumor cell fusions cultured from blood of patients with pancreatic ductal adenocarcinoma.
[So] Source:PLoS One;12(9):e0184451, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we describe isolation and characterization of macrophage-tumor cell fusions (MTFs) from the blood of pancreatic ductal adenocarcinoma (PDAC) patients. The MTFs were generally aneuploidy, and immunophenotypic characterizations showed that the MTFs express markers characteristic of PDAC and stem cells, as well as M2-polarized macrophages. Single cell RNASeq analyses showed that the MTFs express many transcripts implicated in cancer progression, LINE1 retrotransposons, and very high levels of several long non-coding transcripts involved in metastasis (such as MALAT1). When cultured MTFs were transplanted orthotopically into mouse pancreas, they grew as obvious well-differentiated islands of cells, but they also disseminated widely throughout multiple tissues in "stealth" fashion. They were found distributed throughout multiple organs at 4, 8, or 12 weeks after transplantation (including liver, spleen, lung), occurring as single cells or small groups of cells, without formation of obvious tumors or any apparent progression over the 4 to 12 week period. We suggest that MTFs form continually during PDAC development, and that they disseminate early in cancer progression, forming "niches" at distant sites for subsequent colonization by metastasis-initiating cells.
[Mh] Termos MeSH primário: Carcinoma Ductal Pancreático/sangue
Carcinoma Ductal Pancreático/patologia
Macrófagos/patologia
Neoplasias Pancreáticas/sangue
Neoplasias Pancreáticas/patologia
[Mh] Termos MeSH secundário: Animais
Carcinoma Ductal Pancreático/genética
Carcinoma Ductal Pancreático/ultraestrutura
Fusão Celular
Núcleo Celular/patologia
Seres Humanos
Imagem Tridimensional
Imuno-Histoquímica
Imunofenotipagem
Masculino
Camundongos Nus
Microscopia Confocal
Neoplasias Pancreáticas/genética
Neoplasias Pancreáticas/ultraestrutura
Ploidias
Análise de Sequência de RNA
Análise de Célula Única
Células Tumorais Cultivadas
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184451


  10 / 8971 MEDLINE  
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[PMID]:28945747
[Au] Autor:Gentile LB; Nagamine MK; Biondi LR; Sanches DS; Toyota F; Giovani TM; de Jesus IP; da Fonseca IIM; Queiroz-Hazarbassanov N; Diaz BL; Salles Gomes COM; Dagli MLZ
[Ad] Endereço:Laboratory of Experimental and Comparative Oncology, Department of Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, São Paulo, Brazil.
[Ti] Título:Establishment of primary mixed cell cultures from spontaneous canine mammary tumors: Characterization of classic and new cancer-associated molecules.
[So] Source:PLoS One;12(9):e0184228, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There are many factors which make canine cancer like cancer in humans. The occurrence of spontaneous mammary tumors in pet dogs, tumor genetics, molecular targets and exposure to the same environmental risk factors are among these factors. Therefore, the study of canine cancer can provide useful information to the oncology field. This study aimed to establish and characterize a panel of primary mixed cell cultures obtained from spontaneous canine mammary tumors. Eight established cell cultures obtained from one normal mammary gland, one complex adenoma, one mixed adenoma, two complex carcinomas and two mixed carcinomas were analyzed. The gene expression levels of classic molecular cancer players such as fibroblast growth factor receptor (FGFR) 2, breast cancer (BRCA) 1, BRCA2 and estrogen receptor (ESR) 1 were evaluated. For the first time, three orphan nuclear receptors, estrogen-related receptors (ERRs) α, ß and γ were studied in canine mammary cancer. The highest expression level of ERRα was observed in complex carcinoma-derived cell culture, while the highest levels of ERRß and γ were observed in cells derived from a mixed carcinoma. Meanwhile, complex carcinomas presented the highest levels of expression of ESR1, BRCA1 and FGFR2 among all samples. BRCA2 was found exclusively in complex adenoma. The transcription factor GATA3 had its highest levels in mixed carcinoma samples and its lowest levels in complex adenoma. Proliferation assays were also performed to evaluate the mixed cell cultures response to ER ligands, genistein and DES, both in normoxia and hypoxic conditions. Our results demonstrate that morphological and functional studies of primary mixed cell cultures derived from spontaneous canine mammary tumors are possible and provide valuable tool for the study of various stages of mammary cancer development.
[Mh] Termos MeSH primário: Doenças do Cão/metabolismo
Neoplasias Mamárias Animais/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteína BRCA1/metabolismo
Proteína BRCA2/metabolismo
Ciclo Celular
Linhagem Celular Tumoral
Proliferação Celular
Células Cultivadas
Cães
Receptor alfa de Estrogênio/metabolismo
Feminino
Citometria de Fluxo
Ploidias
Cultura Primária de Células
Reação em Cadeia da Polimerase em Tempo Real
Receptor Tipo 2 de Fator de Crescimento de Fibroblastos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BRCA1 Protein); 0 (BRCA2 Protein); 0 (Estrogen Receptor alpha); EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184228



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