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  1 / 3042 MEDLINE  
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[PMID]:29293639
[Au] Autor:Ieda R; Hosoya S; Tajima S; Atsumi K; Kamiya T; Nozawa A; Aoki Y; Tasumi S; Koyama T; Nakamura O; Suzuki Y; Kikuchi K
[Ad] Endereço:Fisheries Laboratory, University of Tokyo, Hamamatsu, Shizuoka, Japan.
[Ti] Título:Identification of the sex-determining locus in grass puffer (Takifugu niphobles) provides evidence for sex-chromosome turnover in a subset of Takifugu species.
[So] Source:PLoS One;13(1):e0190635, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is increasing evidence for frequent turnover in sex chromosomes in vertebrates. Yet experimental systems suitable for tracing the detailed process of turnover are rare. In theory, homologous turnover is possible if the new sex-determining locus is established on the existing sex-chromosome. However, there is no empirical evidence for such an event. The genus Takifugu includes fugu (Takifugu rubripes) and its two closely-related species whose sex is most likely determined by a SNP at the Amhr2 locus. In these species, males are heterozygous, with G and C alleles at the SNP site, while females are homozygous for the C allele. To determine if a shift in the sex-determining locus occurred in another member of this genus, we used genetic mapping to characterize the sex-chromosome systems of Takifugu niphobles. We found that the G allele of Amhr2 is absent in T. niphobles. Nevertheless, our initial mapping suggests a linkage between the phenotypic sex and the chromosome 19, which harbors the Amhr2 locus. Subsequent high-resolution analysis using a sex-reversed fish demonstrated that the sex-determining locus maps to the proximal end of chromosome 19, far from the Amhr2 locus. Thus, it is likely that homologous turnover involving these species has occurred. The data also showed that there is a male-specific reduction of recombination around the sex-determining locus. Nevertheless, no evidence for sex-chromosome differentiation was detected: the reduced recombination depended on phenotypic sex rather than genotypic sex; no X- or Y-specific maker was obtained; the YY individual was viable. Furthermore, fine-scale mapping narrowed down the new sex-determining locus to the interval corresponding to approximately 300-kb of sequence in the fugu genome. Thus, T. niphobles is determined to have a young and small sex-determining region that is suitable for studying an early phase of sex-chromosome evolution and the mechanisms underlying turnover of sex chromosome.
[Mh] Termos MeSH primário: Cromossomos Sexuais
Processos de Determinação Sexual
Takifugu/genética
[Mh] Termos MeSH secundário: Animais
Cruzamentos Genéticos
Feminino
Masculino
Filogenia
Polimorfismo de Nucleotídeo Único
Locos de Características Quantitativas
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190635


  2 / 3042 MEDLINE  
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[PMID]:29186711
[Au] Autor:Viana PF; Ezaz T; Marajó L; Ferreira M; Zuanon J; Cioffi MB; Bertollo LAC; Gross MC; Feldberg E
[Ad] Endereço:Instituto Nacional de Pesquisas da Amazônia, Coordenação de Biodiversidade, Universidade Federal do Amazonas, Manaus, Brazil.
[Ti] Título:Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes).
[So] Source:Cytogenet Genome Res;153(2):96-104, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family.
[Mh] Termos MeSH primário: Cromossomos Sexuais/genética
Processos de Determinação Sexual
Tetraodontiformes/genética
[Mh] Termos MeSH secundário: Animais
Antígenos Nucleares/genética
Brasil
Bandeamento Cromossômico
DNA Ribossômico/genética
Feminino
Amplificação de Genes
Hibridização in Situ Fluorescente
Masculino
RNA Ribossômico 18S/genética
Sequências Repetitivas de Ácido Nucleico
Telômero/genética
Telômero/ultraestrutura
Tropomiosina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Nuclear); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 18S); 0 (Tropomyosin); 0 (nucleolar organizer region associated proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1159/000484423


  3 / 3042 MEDLINE  
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[PMID]:29362783
[Ti] Título:Predetermination of Sex.
[So] Source:JAMA;319(4):413, 2018 01 23.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Drosophila melanogaster/genética
Processos de Determinação Sexual
[Mh] Termos MeSH secundário: Animais
Pesquisa Biomédica/história
Drosophila melanogaster/fisiologia
Feminino
Genótipo
História do Século XX
Seres Humanos
Masculino
Fenômenos Reprodutivos Fisiológicos
Cromossomos Sexuais
Razão de Masculinidade
[Pt] Tipo de publicação:CLASSICAL ARTICLE; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.12215


  4 / 3042 MEDLINE  
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[PMID]:28469017
[Au] Autor:Abbott JK; Nordén AK; Hansson B
[Ad] Endereço:Department of Biology, Lund University, Sölvegatan 37, 223 62 Lund, Sweden jessica.abbott@biol.lu.se.
[Ti] Título:Sex chromosome evolution: historical insights and future perspectives.
[So] Source:Proc Biol Sci;284(1854), 2017 May 17.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many separate-sexed organisms have sex chromosomes controlling sex determination. Sex chromosomes often have reduced recombination, specialized (frequently sex-specific) gene content, dosage compensation and heteromorphic size. Research on sex determination and sex chromosome evolution has increased over the past decade and is today a very active field. However, some areas within the field have not received as much attention as others. We therefore believe that a historic overview of key findings and empirical discoveries will put current thinking into context and help us better understand where to go next. Here, we present a timeline of important conceptual and analytical models, as well as empirical studies that have advanced the field and changed our understanding of the evolution of sex chromosomes. Finally, we highlight gaps in our knowledge so far and propose some specific areas within the field that we recommend a greater focus on in the future, including the role of ecology in sex chromosome evolution and new multilocus models of sex chromosome divergence.
[Mh] Termos MeSH primário: Evolução Molecular
Cromossomos Sexuais/genética
[Mh] Termos MeSH secundário: Animais
Compensação de Dosagem (Genética)
Feminino
Masculino
Processos de Determinação Sexual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


  5 / 3042 MEDLINE  
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[PMID]:28464822
[Au] Autor:Conte MA; Gammerdinger WJ; Bartie KL; Penman DJ; Kocher TD
[Ad] Endereço:Department of Biology, University of Maryland, 20742, College Park, MD, USA.
[Ti] Título:A high quality assembly of the Nile Tilapia (Oreochromis niloticus) genome reveals the structure of two sex determination regions.
[So] Source:BMC Genomics;18(1):341, 2017 05 02.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tilapias are the second most farmed fishes in the world and a sustainable source of food. Like many other fish, tilapias are sexually dimorphic and sex is a commercially important trait in these fish. In this study, we developed a significantly improved assembly of the tilapia genome using the latest genome sequencing methods and show how it improves the characterization of two sex determination regions in two tilapia species. RESULTS: A homozygous clonal XX female Nile tilapia (Oreochromis niloticus) was sequenced to 44X coverage using Pacific Biosciences (PacBio) SMRT sequencing. Dozens of candidate de novo assemblies were generated and an optimal assembly (contig NG50 of 3.3Mbp) was selected using principal component analysis of likelihood scores calculated from several paired-end sequencing libraries. Comparison of the new assembly to the previous O. niloticus genome assembly reveals that recently duplicated portions of the genome are now well represented. The overall number of genes in the new assembly increased by 27.3%, including a 67% increase in pseudogenes. The new tilapia genome assembly correctly represents two recent vasa gene duplication events that have been verified with BAC sequencing. At total of 146Mbp of additional transposable element sequence are now assembled, a large proportion of which are recent insertions. Large centromeric satellite repeats are assembled and annotated in cichlid fish for the first time. Finally, the new assembly identifies the long-range structure of both a ~9Mbp XY sex determination region on LG1 in O. niloticus, and a ~50Mbp WZ sex determination region on LG3 in the related species O. aureus. CONCLUSIONS: This study highlights the use of long read sequencing to correctly assemble recent duplications and to characterize repeat-filled regions of the genome. The study serves as an example of the need for high quality genome assemblies and provides a framework for identifying sex determining genes in tilapia and related fish species.
[Mh] Termos MeSH primário: Ciclídeos/genética
Genômica
Processos de Determinação Sexual/genética
[Mh] Termos MeSH secundário: Animais
Loci Gênicos/genética
Anotação de Sequência Molecular
Sequências Repetitivas de Ácido Nucleico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-017-3723-5


  6 / 3042 MEDLINE  
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[PMID]:29095827
[Au] Autor:Mohamad Ishak NS; Nong QD; Matsuura T; Kato Y; Watanabe H
[Ad] Endereço:Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
[Ti] Título:Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna.
[So] Source:PLoS Genet;13(11):e1006953, 2017 Nov.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Divergence of upstream regulatory pathways of the transcription factor Doublesex (Dsx) serves as a basis for evolution of sex-determining mechanisms in animals. However, little is known about the regulation of Dsx in environmental sex determination. In the crustacean Daphnia magna, environmental sex determination is implemented by male-specific expression of the Dsx ortholog, Dsx1. Transcriptional regulation of Dsx1 comprises at least three phases during embryogenesis: non-sex-specific initiation, male-specific up-regulation, and its maintenance. Herein, we demonstrate that the male-specific up-regulation is controlled by the bZIP transcription factor, Vrille (Vri), an ortholog of the circadian clock genes-Drosophila Vri and mammalian E4BP4/NFIL3. Sequence analysis of the Dsx1 promoter/enhancer revealed a conserved element among two Daphnia species (D. magna and D. pulex), which contains a potential enhancer harboring a consensus Vri binding site overlapped with a consensus Dsx binding site. Besides non-sex-specific expression of Vri in late embryos, we found male-specific expression in early gastrula before the Dsx1 up-regulation phase begins. Knockdown of Vri in male embryos showed reduction of Dsx1 expression. In addition, transient overexpression of Vri in early female embryos up-regulated the expression of Dsx1 and induced male-specific trait. Targeted mutagenesis using CRISPR/Cas9 disrupted the enhancer on genome in males, which led to the reduction of Dsx1 expression. These results indicate that Vri was co-opted as a transcriptional activator of Dsx1 in environmental sex determination of D. magna. The data suggests the remarkably plastic nature of gene regulatory network in sex determination.
[Mh] Termos MeSH primário: Fatores de Transcrição de Zíper de Leucina Básica/metabolismo
Proteínas de Ligação a DNA/metabolismo
Daphnia/genética
Processos de Determinação Sexual/genética
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição de Zíper de Leucina Básica/genética
Sistemas CRISPR-Cas
Clonagem Molecular
Proteínas de Ligação a DNA/genética
Daphnia/embriologia
Elementos Facilitadores Genéticos
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Redes Reguladoras de Genes
Loci Gênicos
Técnicas de Genotipagem
Masculino
Regiões Promotoras Genéticas
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic-Leucine Zipper Transcription Factors); 0 (DNA-Binding Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006953


  7 / 3042 MEDLINE  
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[PMID]:29016690
[Au] Autor:Wu GC; Li HW; Tey WG; Lin CJ; Chang CF
[Ad] Endereço:Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
[Ti] Título:Expression profile of amh/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides.
[So] Source:PLoS One;12(10):e0185864, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gonadal differentiation is tightly regulated by the initial sex determining gene and the downstream sex-related genes in vertebrates. However, sex change in fish can alter the sexual fate from one sex to the other. Chemical-induced maleness in the protogynous orange-spotted grouper is transient, and a reversible sex change occurs after the chemical treatment is withdrawn. We used these characteristics to study Amh signaling during bi-directional sex change in the grouper. We successfully induced the female-to-male sex change by chemical (aromatase inhibitor, AI, or methyltestosterone, MT) treatment. A dormant gonad (a low proliferation rate of early germ cells and no characteristics of both sexes) was found during the transient phase of reversible male-to-female sex change after the withdrawal of chemical administration. Our results showed that amh (anti-mullerian hormone) and its receptor amhr2 (anti-mullerian hormone receptor type 2) were significantly increased in the gonads during the process of female-to-male sex change. Amh is expressed in the Sertoli cells surrounding the type A spermatogonia in the female-to-male grouper. Male-related gene (dmrt1 and sox9) expression was immediately decreased in MT-terminated males during the reversible male-to-female sex change. However, Amh expression was found in the surrounding cells of type A spermatogonia-like cells during the transient phase of reversible male-to-female sex change. This phenomenon is correlated with the dormancy of type A spermatogonia-like cells. Thus, Amh signaling is suggested to play roles in regulating male differentiation during the female-to-male sex change and in inhibiting type-A spermatogonia-like cell proliferation/differentiation during the reversible male-to-female sex change. We suggest that Amh signaling might play dual roles during bi-directional sex change in grouper.
[Mh] Termos MeSH primário: Hormônio Antimülleriano/genética
Inibidores da Aromatase/farmacologia
Regulação da Expressão Gênica no Desenvolvimento
Metiltestosterona/farmacologia
Receptores de Peptídeos/genética
Receptores de Fatores de Crescimento Transformadores beta/genética
Diferenciação Sexual/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Hormônio Antimülleriano/metabolismo
Feminino
Masculino
Ovário/citologia
Ovário/efeitos dos fármacos
Ovário/metabolismo
Perciformes
Receptores de Peptídeos/metabolismo
Receptores de Fatores de Crescimento Transformadores beta/metabolismo
Fatores de Transcrição SOX9/genética
Fatores de Transcrição SOX9/metabolismo
Células de Sertoli/citologia
Células de Sertoli/efeitos dos fármacos
Células de Sertoli/metabolismo
Processos de Determinação Sexual
Diferenciação Sexual/genética
Transdução de Sinais
Espermatogônias/citologia
Espermatogônias/efeitos dos fármacos
Espermatogônias/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aromatase Inhibitors); 0 (DMRT1 protein); 0 (Receptors, Peptide); 0 (Receptors, Transforming Growth Factor beta); 0 (SOX9 Transcription Factor); 0 (Transcription Factors); 0 (anti-Mullerian hormone receptor); 80497-65-0 (Anti-Mullerian Hormone); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185864


  8 / 3042 MEDLINE  
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[PMID]:28976974
[Au] Autor:Sawala A; Gould AP
[Ad] Endereço:The Francis Crick Institute, London, United Kingdom.
[Ti] Título:The sex of specific neurons controls female body growth in Drosophila.
[So] Source:PLoS Biol;15(10):e2002252, 2017 Oct.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sexual dimorphisms in body size are widespread throughout the animal kingdom but their underlying mechanisms are not well characterized. Most models for how sex chromosome genes specify size dimorphism have emphasized the importance of gonadal hormones and cell-autonomous influences in mammals versus strictly cell-autonomous mechanisms in Drosophila melanogaster. Here, we use tissue-specific genetics to investigate how sexual size dimorphism (SSD) is established in Drosophila. We find that the larger body size characteristic of Drosophila females is established very early in larval development via an increase in the growth rate per unit of body mass. We demonstrate that the female sex determination gene, Sex-lethal (Sxl), functions in central nervous system (CNS) neurons as part of a relay that specifies the early sex-specific growth trajectories of larval but not imaginal tissues. Neuronal Sxl acts additively in 2 neuronal subpopulations, one of which corresponds to 7 median neurosecretory cells: the insulin-producing cells (IPCs). Surprisingly, however, male-female differences in the production of insulin-like peptides (Ilps) from the IPCs do not appear to be involved in establishing SSD in early larvae, although they may play a later role. These findings support a relay model in which Sxl in neurons and Sxl in local tissues act together to specify the female-specific growth of the larval body. They also reveal that, even though the sex determination pathways in Drosophila and mammals are different, they both modulate body growth via a combination of tissue-autonomous and nonautonomous inputs.
[Mh] Termos MeSH primário: Drosophila/crescimento & desenvolvimento
Neurônios/fisiologia
Processos de Determinação Sexual/genética
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal/genética
Drosophila/genética
Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Proteínas de Drosophila/fisiologia
Ingestão de Alimentos
Feminino
Neurônios GABAérgicos/fisiologia
Células Secretoras de Insulina/metabolismo
Larva
Masculino
Proteínas de Ligação a RNA/genética
Proteínas de Ligação a RNA/fisiologia
Caracteres Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (ILP2 protein, Drosophila); 0 (RNA-Binding Proteins); 0 (Sxl protein, Drosophila)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2002252


  9 / 3042 MEDLINE  
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[PMID]:28928204
[Au] Autor:Miyauchi H; Ohta H; Nagaoka S; Nakaki F; Sasaki K; Hayashi K; Yabuta Y; Nakamura T; Yamamoto T; Saitou M
[Ad] Endereço:Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
[Ti] Título:Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice.
[So] Source:EMBO J;36(21):3100-3119, 2017 Nov 02.
[Is] ISSN:1460-2075
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mechanism for sex determination in mammalian germ cells remains unclear. Here, we reconstitute the female sex determination in mouse germ cells under a defined condition without the use of gonadal somatic cells. We show that retinoic acid (RA) and its key effector, STRA8, are not sufficient to induce the female germ-cell fate. In contrast, bone morphogenetic protein (BMP) and RA synergistically induce primordial germ cells (PGCs)/PGC-like cells (PGCLCs) derived from embryonic stem cells (ESCs) into fetal primary oocytes. The induction is characterized by entry into the meiotic prophase, occurs synchronously and recapitulates cytological and transcriptome progression faithfully. Importantly, the female germ-cell induction necessitates a proper cellular competence-most typically, DNA demethylation of relevant genes-which is observed in appropriately propagated PGCs/PGCLCs, but not in PGCs/PGCLCs immediately after induction. This provides an explanation for the differential function of BMP signaling between PGC specification and female germ-cell induction. Our findings represent a framework for a comprehensive delineation of the sex-determination pathway in mammalian germ cells, including humans.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/genética
Proteínas Morfogenéticas Ósseas/farmacologia
Regulação da Expressão Gênica no Desenvolvimento
Células-Tronco Embrionárias Murinas/efeitos dos fármacos
Oócitos/efeitos dos fármacos
Tretinoína/farmacologia
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Proteínas Morfogenéticas Ósseas/genética
Proteínas Morfogenéticas Ósseas/metabolismo
Diferenciação Celular
Feminino
Feto
Perfilação da Expressão Gênica
Genes Reporter
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Camundongos
Camundongos Endogâmicos ICR
Células-Tronco Embrionárias Murinas/citologia
Células-Tronco Embrionárias Murinas/metabolismo
Oócitos/citologia
Oócitos/crescimento & desenvolvimento
Oócitos/metabolismo
Fator 1 de Ligação ao Domínio I Regulador Positivo
Prófase
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Processos de Determinação Sexual
Transdução de Sinais
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Bacterial Proteins); 0 (Bone Morphogenetic Proteins); 0 (Luminescent Proteins); 0 (Prdm1 protein, mouse); 0 (Protein Isoforms); 0 (Stra8 protein, mouse); 0 (Transcription Factors); 0 (yellow fluorescent protein, Bacteria); 5688UTC01R (Tretinoin); EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.15252/embj.201796875


  10 / 3042 MEDLINE  
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[PMID]:28911174
[Au] Autor:Hirst CE; Major AT; Ayers KL; Brown RJ; Mariette M; Sackton TB; Smith CA
[Ad] Endereço:Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia.
[Ti] Título:Sex Reversal and Comparative Data Undermine the W Chromosome and Support Z-linked DMRT1 as the Regulator of Gonadal Sex Differentiation in Birds.
[So] Source:Endocrinology;158(9):2970-2987, 2017 Sep 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The exact genetic mechanism regulating avian gonadal sex differentiation has not been completely resolved. The most likely scenario involves a dosage mechanism, whereby the Z-linked DMRT1 gene triggers testis development. However, the possibility still exists that the female-specific W chromosome may harbor an ovarian determining factor. In this study, we provide evidence that the universal gene regulating gonadal sex differentiation in birds is Z-linked DMRT1 and not a W-linked (ovarian) factor. Three candidate W-linked ovarian determinants are HINTW, female-expressed transcript 1 (FET1), and female-associated factor (FAF). To test the association of these genes with ovarian differentiation in the chicken, we examined their expression following experimentally induced female-to-male sex reversal using the aromatase inhibitor fadrozole (FAD). Administration of FAD on day 3 of embryogenesis induced a significant loss of aromatase enzyme activity in female gonads and masculinization. However, expression levels of HINTW, FAF, and FET1 were unaltered after experimental masculinization. Furthermore, comparative analysis showed that FAF and FET1 expression could not be detected in zebra finch gonads. Additionally, an antibody raised against the predicted HINTW protein failed to detect it endogenously. These data do not support a universal role for these genes or for the W sex chromosome in ovarian development in birds. We found that DMRT1 (but not the recently identified Z-linked HEMGN gene) is male upregulated in embryonic zebra finch and emu gonads, as in the chicken. As chicken, zebra finch, and emu exemplify the major evolutionary clades of birds, we propose that Z-linked DMRT1, and not the W sex chromosome, regulates gonadal sex differentiation in birds.
[Mh] Termos MeSH primário: Aves
Cromossomos Sexuais/genética
Processos de Determinação Sexual/genética
Diferenciação Sexual/genética
Fatores de Transcrição/fisiologia
[Mh] Termos MeSH secundário: Animais
Aves/embriologia
Aves/genética
Embrião de Galinha
Galinhas
Desenvolvimento Embrionário
Feminino
Feminização/embriologia
Feminização/genética
Tentilhões/embriologia
Tentilhões/genética
Regulação da Expressão Gênica no Desenvolvimento
Gônadas
Masculino
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DMRT1 protein); 0 (Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00316



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