Base de dados : MEDLINE
Pesquisa : G05.935.500 [Categoria DeCS]
Referências encontradas : 4760 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 476 ir para página                         

  1 / 4760 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28911112
[Au] Autor:Cury J; Touchon M; Rocha EPC
[Ad] Endereço:Microbial Evolutionary Genomics, Institut Pasteur, 28, rue du Dr Roux, Paris 75015, France.
[Ti] Título:Integrative and conjugative elements and their hosts: composition, distribution and organization.
[So] Source:Nucleic Acids Res;45(15):8943-8956, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Conjugation of single-stranded DNA drives horizontal gene transfer between bacteria and was widely studied in conjugative plasmids. The organization and function of integrative and conjugative elements (ICE), even if they are more abundant, was only studied in a few model systems. Comparative genomics of ICE has been precluded by the difficulty in finding and delimiting these elements. Here, we present the results of a method that circumvents these problems by requiring only the identification of the conjugation genes and the species' pan-genome. We delimited 200 ICEs and this allowed the first large-scale characterization of these elements. We quantified the presence in ICEs of a wide set of functions associated with the biology of mobile genetic elements, including some that are typically associated with plasmids, such as partition and replication. Protein sequence similarity networks and phylogenetic analyses revealed that ICEs are structured in functional modules. Integrases and conjugation systems have different evolutionary histories, even if the gene repertoires of ICEs can be grouped in function of conjugation types. Our characterization of the composition and organization of ICEs paves the way for future functional and evolutionary analyses of their cargo genes, composed of a majority of unknown function genes.
[Mh] Termos MeSH primário: Conjugação Genética
Elementos de DNA Transponíveis
DNA Bacteriano/genética
Transferência Genética Horizontal
Filogenia
Plasmídeos/química
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Actinobacteria/genética
Actinobacteria/metabolismo
Archaea/classificação
Archaea/genética
Archaea/metabolismo
Replicação do DNA
DNA Bacteriano/metabolismo
Evolução Molecular
Firmicutes/classificação
Firmicutes/genética
Firmicutes/metabolismo
Genes Bacterianos
Integrases/genética
Integrases/metabolismo
Lisogenia
Plasmídeos/metabolismo
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/metabolismo
Recombinases/genética
Recombinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial); 0 (Recombinases); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx607


  2 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28901529
[Au] Autor:Joshi H; Seniya SP; Suryanarayanan V; Patidar ND; Singh SK; Jain V
[Ad] Endereço:Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal, India.
[Ti] Título:Dissecting the structure-function relationship in lysozyme domain of mycobacteriophage D29-encoded peptidoglycan hydrolase.
[So] Source:FEBS Lett;591(20):3276-3287, 2017 Oct.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Most bacteriophages rapidly infect and kill bacteria and, therefore, qualify as the next generation therapeutics for rapidly emerging drug-resistant bacteria such as Mycobacterium tuberculosis. We have previously characterized the mycobacteriophage D29-generated endolysin, Lysin A, for its activity against mycobacteria. Here, we present a detailed characterization of the lysozyme domain (LD) of D29 Lysin A that hydrolyzes peptidoglycan of both gram-positive and gram-negative bacteria with high potency. By characterizing an exhaustive LD protein variant library, we have identified critical residues important for LD activity and stability. We further complement our in vitro experiments with detailed in silico investigations. We present LD as a potent candidate for developing phage-based broad-spectrum therapeutics.
[Mh] Termos MeSH primário: Endopeptidases/química
Lisogenia/genética
Muramidase/química
N-Acetil-Muramil-L-Alanina Amidase/química
Proteínas Virais/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Domínio Catalítico
Clonagem Molecular
Endopeptidases/genética
Endopeptidases/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Cinética
Ligantes
Simulação de Dinâmica Molecular
Muramidase/genética
Muramidase/metabolismo
Mutação
Micobacteriófagos/química
Micobacteriófagos/genética
Micobacteriófagos/patogenicidade
Mycobacterium tuberculosis/virologia
N-Acetil-Muramil-L-Alanina Amidase/genética
N-Acetil-Muramil-L-Alanina Amidase/metabolismo
Biblioteca de Peptídeos
Ligação Proteica
Conformação Proteica em alfa-Hélice
Domínios Proteicos
Domínios e Motivos de Interação entre Proteínas
Estabilidade Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Relação Estrutura-Atividade
Especificidade por Substrato
Termodinâmica
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Ligands); 0 (Peptide Library); 0 (Recombinant Proteins); 0 (Viral Proteins); EC 3.2.1.17 (Muramidase); EC 3.4.- (Endopeptidases); EC 3.4.99.- (endolysin); EC 3.5.1.28 (N-Acetylmuramoyl-L-alanine Amidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12848


  3 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873456
[Au] Autor:Stough JMA; Tang X; Krausfeldt LE; Steffen MM; Gao G; Boyer GL; Wilhelm SW
[Ad] Endereço:Department of Microbiology, University of Tennessee, Knoxville, TN, United States of America.
[Ti] Título:Molecular prediction of lytic vs lysogenic states for Microcystis phage: Metatranscriptomic evidence of lysogeny during large bloom events.
[So] Source:PLoS One;12(9):e0184146, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microcystis aeruginosa is a freshwater bloom-forming cyanobacterium capable of producing the potent hepatotoxin, microcystin. Despite increased interest in this organism, little is known about the viruses that infect it and drive nutrient mobilization and transfer of genetic material between organisms. The genomic complement of sequenced phage suggests these viruses are capable of integrating into the host genome, though this activity has not been observed in the laboratory. While analyzing RNA-sequence data obtained from Microcystis blooms in Lake Tai (Taihu, China), we observed that a series of lysogeny-associated genes were highly expressed when genes involved in lytic infection were down-regulated. This pattern was consistent, though not always statistically significant, across multiple spatial and temporally distinct samples. For example, samples from Lake Tai (2014) showed a predominance of lytic virus activity from late July through October, while genes associated with lysogeny were strongly expressed in the early months (June-July) and toward the end of bloom season (October). Analyses of whole phage genome expression shows that transcription patterns are shared across sampling locations and that genes consistently clustered by co-expression into lytic and lysogenic groups. Expression of lytic-cycle associated genes was positively correlated to total dissolved nitrogen, ammonium concentration, and salinity. Lysogeny-associated gene expression was positively correlated with pH and total dissolved phosphorous. Our results suggest that lysogeny may be prevalent in Microcystis blooms and support the hypothesis that environmental conditions drive switching between temperate and lytic life cycles during bloom proliferation.
[Mh] Termos MeSH primário: Bacteriófagos/genética
Eutrofização
Lisogenia/genética
Microcystis/virologia
Transcriptoma/genética
[Mh] Termos MeSH secundário: Meio Ambiente
Perfilação da Expressão Gênica
Regulação Viral da Expressão Gênica
Genoma Viral
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184146


  4 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28800363
[Au] Autor:Czajkowski R; Smolarska A; Ozymko Z
[Ad] Endereço:Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
[Ti] Título:The viability of lytic bacteriophage ΦD5 in potato-associated environments and its effect on Dickeya solani in potato (Solanum tuberosum L.) plants.
[So] Source:PLoS One;12(8):e0183200, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dickeya solani is one of the most important pectinolytic phytopathogens responsible for high losses in potato, especially in seed potato production in Europe. Lytic bacteriophages can affect the structure of the host population and may influence spread, survival and virulence of the pathogen and in consequence, infection of the plant. In this study, we aimed to acquire information on the viability of the broad host lytic bacteriophage ΦD5 on potato, as well as to apprehend the specific effect of this bacteriophage on its host D. solani type-strain in different settings, as a preliminary step to target co-adaptation of phages and host bacteria in plant environment. Viability of the ΦD5 phage in tuber extract, on tuber surface, in potting compost, in rainwater and on the leaf surface, as well as the effect of copper sulfate, were examined under laboratory conditions. Also, the interaction of ΦD5 with the target host D. solani in vitro and in compost-grown potato plants was evaluated. ΦD5 remained infectious in potato tuber extract and rain water for up to 72 h but was inactivated in solutions containing 50 mM of copper. The phage population was stable for up to 28 days on potato tuber surface and in potting compost. In both, tissue culture and compost-grown potato plants, ΦD5 reduced infection by D. solani by more than 50%. The implications of these findings are discussed.
[Mh] Termos MeSH primário: Bacteriófagos/efeitos dos fármacos
Sulfato de Cobre/farmacologia
Lisogenia/efeitos dos fármacos
Pectobacterium/virologia
[Mh] Termos MeSH secundário: Bacteriófagos/fisiologia
Lisogenia/fisiologia
Pectobacterium/crescimento & desenvolvimento
Pectobacterium/patogenicidade
Doenças das Plantas/microbiologia
Doenças das Plantas/prevenção & controle
Extratos Vegetais/farmacologia
Tubérculos/efeitos dos fármacos
Tubérculos/microbiologia
Tubérculos/virologia
Solo/química
Solanum tuberosum/efeitos dos fármacos
Solanum tuberosum/microbiologia
Solanum tuberosum/virologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Soil); LRX7AJ16DT (Copper Sulfate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183200


  5 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28409265
[Au] Autor:Lim JA; Heu S; Park J; Roh E
[Ad] Endereço:Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, 55365, Republic of Korea.
[Ti] Título:Genomic characterization of bacteriophage vB_PcaP_PP2 infecting Pectobacterium carotovorum subsp. carotovorum, a new member of a proposed genus in the subfamily Autographivirinae.
[So] Source:Arch Virol;162(8):2441-2444, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bacteriophage vB_PcaP_PP2 (PP2) is a novel virulent phage that infects the plant-pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. PP2 phage has a 41,841-bp double-stranded DNA encoding 47 proteins, and it was identified as a member of the family Podoviridae by transmission electron microscopy. Nineteen of its open reading frames (ORFs) show homology to functional proteins, and 28 ORFs have been characterized as hypothetical proteins. PP2 phage is homologous to Cronobacter phage vB_CskP_GAP227 and Dev-CD-23823. Based on phylogenetic analysis, PP2 and its homologous bacteriophages form a new group within the subfamily Autographivirinae in the family Podoviridae, suggesting the need to establish a new genus. No lysogenic-cycle-related genes or bacterial toxins were identified.
[Mh] Termos MeSH primário: Bacteriófagos/genética
Genoma Viral
Pectobacterium/virologia
Podoviridae/classificação
Podoviridae/genética
[Mh] Termos MeSH secundário: Toxinas Bacterianas/genética
Bacteriófagos/classificação
Bacteriófagos/isolamento & purificação
Bacteriófagos/patogenicidade
DNA Viral/genética
Lisogenia/genética
Microscopia Eletrônica de Transmissão
Fases de Leitura Aberta
Filogenia
Plantas/microbiologia
Podoviridae/isolamento & purificação
Podoviridae/ultraestrutura
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3349-6


  6 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28399796
[Au] Autor:Wendling CC; Piecyk A; Refardt D; Chibani C; Hertel R; Liesegang H; Bunk B; Overmann J; Roth O
[Ad] Endereço:GEOMAR, Helmholtz Centre for Ocean Research, Evolutionary Ecology of Marine Fishes, Düsternbrooker Weg 20, 24105, Kiel, Germany. cwendling@geomar.de.
[Ti] Título:Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria.
[So] Source:BMC Evol Biol;17(1):98, 2017 Apr 11.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Evolutionary shifts in bacterial virulence are often associated with a third biological player, for instance temperate phages, that can act as hyperparasites. By integrating as prophages into the bacterial genome they can contribute accessory genes, which can enhance the fitness of their prokaryotic carrier (lysogenic conversion). Hyperparasitic influence in tripartite biotic interactions has so far been largely neglected in empirical host-parasite studies due to their inherent complexity. Here we experimentally address whether bacterial resistance to phages and bacterial harm to eukaryotic hosts is linked using a natural tri-partite system with bacteria of the genus Vibrio, temperate vibriophages and the pipefish Syngnathus typhle. We induced prophages from all bacterial isolates and constructed a three-fold replicated, fully reciprocal 75 × 75 phage-bacteria infection matrix. RESULTS: According to their resistance to phages, bacteria could be grouped into three distinct categories: highly susceptible (HS-bacteria), intermediate susceptible (IS-bacteria), and resistant (R-bacteria). We experimentally challenged pipefish with three selected bacterial isolates from each of the three categories and determined the amount of viable Vibrio counts from infected pipefish and the expression of pipefish immune genes. While the amount of viable Vibrio counts did not differ between bacterial groups, we observed a significant difference in relative gene expression between pipefish infected with phage susceptible and phage resistant bacteria. CONCLUSION: These findings suggest that bacteria with a phage-susceptible phenotype are more harmful against a eukaryotic host, and support the importance of hyperparasitism and the need for an integrative view across more than two levels when studying host-parasite evolution.
[Mh] Termos MeSH primário: Bacteriófagos/fisiologia
Evolução Biológica
Doenças dos Peixes/virologia
Peixes
Vibrioses/veterinária
Vibrio/virologia
[Mh] Termos MeSH secundário: Animais
Bacteriófagos/genética
Peixes/classificação
Genoma Bacteriano
Interações Hospedeiro-Patógeno
Lisogenia
Filogenia
Prófagos
Vibrio/genética
Vibrio/imunologia
Vibrioses/virologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-0930-2


  7 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28302859
[Au] Autor:Diard M; Bakkeren E; Cornuault JK; Moor K; Hausmann A; Sellin ME; Loverdo C; Aertsen A; Ackermann M; De Paepe M; Slack E; Hardt WD
[Ad] Endereço:Institute of Microbiology, ETH Zurich, Switzerland. mederic.diard@micro.biol.ethz.ch wolf-dietrich.hardt@micro.biol.ethz.ch.
[Ti] Título:Inflammation boosts bacteriophage transfer between spp.
[So] Source:Science;355(6330):1211-1215, 2017 03 17.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. A murine Typhimurium ( Tm) diarrhea model was used to study the transfer of SopEΦ, a prophage from Tm SL1344, to Tm ATCC14028S. Gut inflammation and enteric disease triggered >55% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEΦ transfer was reduced by up to 10 -fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal Tm population from inducing inflammation and consequently abolished SopEΦ transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.
[Mh] Termos MeSH primário: Diarreia/microbiologia
Diarreia/patologia
Enterite/microbiologia
Lisogenia
Fagos de Salmonella/patogenicidade
Salmonella typhimurium/patogenicidade
Salmonella typhimurium/virologia
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Enterite/prevenção & controle
Inflamação/microbiologia
Inflamação/prevenção & controle
Intestinos/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Resposta SOS (Genética)
Fagos de Salmonella/genética
Vacinação
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1126/science.aaf8451


  8 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28202757
[Au] Autor:Selb R; Derntl C; Klein R; Alte B; Hofbauer C; Kaufmann M; Beraha J; Schöner L; Witte A
[Ad] Endereço:Department of Microbiology, Immunobiology and Genetics, MFPL Laboratories, University of Vienna, Vienna, Austria.
[Ti] Título:The Viral Gene ORF79 Encodes a Repressor Regulating Induction of the Lytic Life Cycle in the Haloalkaliphilic Virus Ï•Ch1.
[So] Source:J Virol;91(9), 2017 May 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we describe the construction of the first genetically modified mutant of a halovirus infecting haloalkaliphilic By random choice, we targeted ORF79, a currently uncharacterized viral gene of the haloalkaliphilic virus Ï•Ch1. We used a polyethylene glycol (PEG)-mediated transformation method to deliver a disruption cassette into a lysogenic strain of the haloalkaliphilic archaeon bearing Ï•Ch1 as a provirus. This approach yielded mutant virus particles carrying a disrupted version of ORF79. Disruption of ORF79 did not influence morphology of the mature virions. The mutant virus was able to infect cured strains of , resulting in a lysogenic, ORF79-disrupted strain. Analysis of this strain carrying the mutant virus revealed a repressor function of ORF79. In the absence of gp79, onset of lysis and expression of viral proteins occurred prematurely compared to their timing in the wild-type strain. Constitutive expression of ORF79 in a cured strain of reduced the plating efficiency of Ï•Ch1 by seven orders of magnitude. Overexpression of ORF79 in a lysogenic strain of resulted in an inhibition of lysis and total absence of viral proteins as well as viral progeny. In further experiments, gp79 directly regulated the expression of the tail fiber protein ORF34 but did not influence the methyltransferase gene ORF94. Further, we describe the establishment of an inducible promoter for studies in Genetic analyses of haloalkaliphilic or haloviruses are only rarely reported. Therefore, only little insight into the roles of proteins and their functions has been gained so far. We used a reverse genetics approach to identify the function of a yet undescribed gene of Ï•Ch1. We provide evidence that gp79, a currently unknown protein of Ï•Ch1, acts as a repressor protein of the viral life cycle, affecting the transition from the lysogenic to the lytic state of the virus. Thus, repressor genes in other haloviruses could be identified by sequence homologies to gp79 in the future. Moreover, we describe the use of an inducible promoter of Our work provides valuable tools for the identification of other unknown viral genes by our approach as well as for functional studies of proteins by inducible expression.
[Mh] Termos MeSH primário: Halobacteriaceae/virologia
Lisogenia/genética
Myoviridae/genética
Fases de Leitura Aberta/genética
Proteínas Repressoras/genética
[Mh] Termos MeSH secundário: DNA Viral/genética
Genes Virais/genética
Regiões Promotoras Genéticas/genética
Proteínas Virais/genética
Fenômenos Fisiológicos Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Repressor Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE


  9 / 4760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28118395
[Au] Autor:Mikalová L; Bosák J; Hríbková H; Dedicová D; Benada O; Smarda J; Smajs D
[Ad] Endereço:Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic.
[Ti] Título:Novel Temperate Phages of Salmonella enterica subsp. salamae and subsp. diarizonae and Their Activity against Pathogenic S. enterica subsp. enterica Isolates.
[So] Source:PLoS One;12(1):e0170734, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family.
[Mh] Termos MeSH primário: Fagos de Salmonella/isolamento & purificação
Salmonella enterica/virologia
[Mh] Termos MeSH secundário: Tchecoslováquia
DNA Viral/genética
DNA Viral/isolamento & purificação
Microbiologia Ambiental
Genoma Viral
Lisogenia
Microscopia Eletrônica
Filogenia
Infecções por Salmonella/microbiologia
Fagos de Salmonella/classificação
Fagos de Salmonella/fisiologia
Fagos de Salmonella/ultraestrutura
Salmonella enterica/isolamento & purificação
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
Especificidade da Espécie
Carga Viral
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170734


  10 / 4760 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28099413
[Au] Autor:Erez Z; Steinberger-Levy I; Shamir M; Doron S; Stokar-Avihail A; Peleg Y; Melamed S; Leavitt A; Savidor A; Albeck S; Amitai G; Sorek R
[Ad] Endereço:Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
[Ti] Título:Communication between viruses guides lysis-lysogeny decisions.
[So] Source:Nature;541(7638):488-493, 2017 01 26.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Temperate viruses can become dormant in their host cells, a process called lysogeny. In every infection, such viruses decide between the lytic and the lysogenic cycles, that is, whether to replicate and lyse their host or to lysogenize and keep the host viable. Here we show that viruses (phages) of the SPbeta group use a small-molecule communication system to coordinate lysis-lysogeny decisions. During infection of its Bacillus host cell, the phage produces a six amino-acids-long communication peptide that is released into the medium. In subsequent infections, progeny phages measure the concentration of this peptide and lysogenize if the concentration is sufficiently high. We found that different phages encode different versions of the communication peptide, demonstrating a phage-specific peptide communication code for lysogeny decisions. We term this communication system the 'arbitrium' system, and further show that it is encoded by three phage genes: aimP, which produces the peptide; aimR, the intracellular peptide receptor; and aimX, a negative regulator of lysogeny. The arbitrium system enables a descendant phage to 'communicate' with its predecessors, that is, to estimate the amount of recent previous infections and hence decide whether to employ the lytic or lysogenic cycle.
[Mh] Termos MeSH primário: Bacteriólise
Bacteriófagos/fisiologia
Lisogenia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacillus/citologia
Bacillus/virologia
Bacteriólise/efeitos dos fármacos
Bacteriófagos/efeitos dos fármacos
Meios de Cultivo Condicionados/química
Meios de Cultivo Condicionados/farmacologia
DNA Viral/metabolismo
Lisogenia/efeitos dos fármacos
Modelos Biológicos
Peptídeos/química
Peptídeos/farmacologia
Peptídeos/secreção
Multimerização Proteica
Transcrição Genética/efeitos dos fármacos
Proteínas Virais/química
Proteínas Virais/farmacologia
Proteínas Virais/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media, Conditioned); 0 (DNA, Viral); 0 (Peptides); 0 (Viral Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1038/nature21049



página 1 de 476 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde