Base de dados : MEDLINE
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[PMID]:28542462
[Au] Autor:Battin C; Hennig A; Mayrhofer P; Kunert R; Zlabinger GJ; Steinberger P; Paster W
[Ad] Endereço:Division of Immune Receptors and T Cell Activation, Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
[Ti] Título:A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples.
[So] Source:PLoS One;12(5):e0178220, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sensing of pathogens by innate immune cells is essential for the initiation of appropriate immune responses. Toll-like receptors (TLRs), which are highly sensitive for various structurally and evolutionary conserved molecules derived from microbes have a prominent role in this process. TLR engagement results in the activation of the transcription factor NF-κB, which induces the expression of cytokines and other inflammatory mediators. The exquisite sensitivity of TLR signalling can be exploited for the detection of bacteria and microbial contaminants in tissue cultures and in protein preparations. Here we describe a cellular reporter system for the detection of TLR ligands in biological samples. The well-characterized human monocytic THP-1 cell line was chosen as host for an NF-á´‹B-inducible enhanced green fluorescent protein reporter gene. We studied the sensitivity of the resultant reporter cells for a variety of microbial components and observed a strong reactivity towards TLR1/2 and TLR2/6 ligands. Mycoplasma lipoproteins are potent TLR2/6 agonists and we demonstrate that our reporter cells can be used as reliable and robust detection system for mycoplasma contaminations in cell cultures. In addition, a TLR4-sensitive subline of our reporters was engineered, and probed with recombinant proteins expressed in different host systems. Bacterially expressed but not mammalian expressed proteins induced strong reporter activity. We also tested proteins expressed in an E. coli strain engineered to lack TLR4 agonists. Such preparations also induced reporter activation in THP-1 cells highlighting the importance of testing recombinant protein preparations for microbial contaminations beyond endotoxins. Our results demonstrate the usefulness of monocytic reporter cells for high-throughput screening for microbial contaminations in diverse biological samples, including tissue culture supernatants and recombinant protein preparations. Fluorescent reporter assays can be measured on standard flow cytometers and in contrast to established detection methods, like luciferase-based systems or Limulus Amebocyte Lysate tests, they do not require costly reagents.
[Mh] Termos MeSH primário: Fenômenos Microbiológicos
NF-kappa B/metabolismo
Receptores Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular
Meios de Cultura
Proteínas de Escherichia coli/imunologia
Proteínas de Escherichia coli/metabolismo
Citometria de Fluxo
Corantes Fluorescentes/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Ensaios de Triagem em Larga Escala
Seres Humanos
Imunidade Inata
Ligantes
Monócitos/imunologia
Monócitos/metabolismo
Receptores de Reconhecimento de Padrão/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Receptor 4 Toll-Like/agonistas
Receptores Toll-Like/agonistas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Escherichia coli Proteins); 0 (Fluorescent Dyes); 0 (Ligands); 0 (NF-kappa B); 0 (Receptors, Pattern Recognition); 0 (Recombinant Proteins); 0 (TLR4 protein, human); 0 (Toll-Like Receptor 4); 0 (Toll-Like Receptors); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178220


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[PMID]:28418794
[Au] Autor:Mediano P; Fernández L; Jiménez E; Arroyo R; Espinosa-Martos I; Rodríguez JM; Marín M
[Ad] Endereço:1 Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Madrid, Spain.
[Ti] Título:Microbial Diversity in Milk of Women With Mastitis: Potential Role of Coagulase-Negative Staphylococci, Viridans Group Streptococci, and Corynebacteria.
[So] Source:J Hum Lact;33(2):309-318, 2017 May.
[Is] ISSN:1552-5732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lactational mastitis constitutes a significant cause of premature weaning. However, its etiology, linked to the presence of pathogenic microorganisms, has been scarcely reported. Research aim: The aim of this study was to describe the microbial diversity in milk samples from women suffering from lactational mastitis and to identify more accurately a collection of isolates belonging to coagulase-negative staphylococci, streptococci, and coryneform bacteria. METHODS: This is a cross-sectional descriptive one-group study. A total of 5,009 isolates from 1,849 mastitis milk samples was identified by culture, biochemical, and/or molecular methods at the species or genus level. A more precise identification of a collection of 211 isolates was carried out by 16S rRNA gene sequencing. RESULTS: Mean total bacterial count in milk samples was 4.11 log colony-forming units/ml, 95% confidence interval [4.08, 4.15]. Staphylococcus epidermidis was the most common species being isolated from 91.56% of the samples, whereas Staphylococcus aureus was detected in 29.74%. Streptococci and corynebacteria constituted the second (70.20%) and third (16.60%) most prevalent bacterial groups, respectively, found in this study. In contrast, Candida spp. was present in only 0.54% of the samples. Sequencing of the 16S rRNA gene revealed a high diversity of bacterial species among identified isolates. CONCLUSION: Many coagulase-negative staphylococci, viridans group streptococci, and corynebacteria, usually dismissed as contaminant bacteria, may play an important role as etiologic agents of mastitis. Proper diagnosis of mastitis should be established after performing microbiological testing of milk based on standardized procedures. A reliable analysis must identify the mastitis-causing pathogen(s) at the species level and its(their) concentration(s).
[Mh] Termos MeSH primário: Mastite/microbiologia
Fenômenos Microbiológicos
Leite Humano/microbiologia
[Mh] Termos MeSH secundário: Adulto
Aleitamento Materno/efeitos adversos
Corynebacterium/patogenicidade
Estudos Transversais
Feminino
Seres Humanos
Lactação/metabolismo
RNA Ribossômico 16S/análise
Espanha
Infecções Estafilocócicas/complicações
Infecções Estafilocócicas/genética
Staphylococcus aureus/patogenicidade
Staphylococcus epidermidis/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1177/0890334417692968


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[PMID]:27902434
[Au] Autor:Kirkpatrick CL; Lesouhaitier O; Malone JG; An SQ; Caly DL
[Ad] Endereço:1​Department of Microbiology & Molecular Medicine, Institute of Genetics & Genomics in Geneva (iGE3), Faculty of Medicine/University Medical Centre, University of Geneva, Geneva, Switzerland.
[Ti] Título:Interaction and signalling networks: a report from the fourth 'Young Microbiologists Symposium on Microbe Signalling, Organisation and Pathogenesis'.
[So] Source:Microbiology;163(1):4-8, 2017 Jan.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:At the end of June, over 120 microbiologists from 18 countries gathered in Dundee, Scotland for the fourth edition of the Young Microbiologists Symposium on 'Microbe Signalling, Organisation and Pathogenesis'. The aim of the symposium was to give early career microbiologists the opportunity to present their work in a convivial environment and to interact with senior world-renowned scientists in exciting fields of microbiology research. The meeting was supported by the Microbiology Society, the Society of Applied Microbiology and the American Society for Microbiology with further sponsorship from the European Molecular Biology Organisation and the Royal Society of Edinburgh. In this report, we highlight some themes that emerged from the many interesting talks and poster presentations, as well as some of the other activities that were on offer at this energetic meeting.
[Mh] Termos MeSH primário: Bactérias/patogenicidade
Interações Hospedeiro-Patógeno/fisiologia
Fenômenos Microbiológicos
[Mh] Termos MeSH secundário: Bactérias/enzimologia
Seres Humanos
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
[Pt] Tipo de publicação:CONGRESSES
[Nm] Nome de substância:
0 (Intracellular Signaling Peptides and Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.000401


  4 / 132 MEDLINE  
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[PMID]:27894932
[Au] Autor:Martani F; Berterame NM; Branduardi P
[Ad] Endereço:University of Milano-Bicocca, Department of Biotechnology and Biosciences, Milano, Italy. Electronic address: francesca.martani@unimib.it.
[Ti] Título:Microbial stress: From molecules to systems (Sitges, November 2015).
[So] Source:N Biotechnol;35:30-34, 2017 Mar 25.
[Is] ISSN:1876-4347
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The meeting "Microbial Stress: from Molecules to Systems" - the third in this series - was held in Sitges (Spain) in November 2015. The meeting offered the opportunity for international scientists to share their viewpoints and recent outcomes concerning microbial stress responses. Particular attention was given to the characterisation of mechanisms triggered by stress, from detailed molecular biology through whole organism systems biology up to the level of populations. A deeper understanding of microbial responses to stress is indeed attainable only considering the phenomenon as a whole. Exhaustive knowledge of the various stress response systems, and of their interconnections, is important for different applications, from the prevention and counteraction of bacterial infectious diseases to the engineering of robust cell factories. The presentations covered all of these aspects, enabling an active interaction among participants. It also stimulated discussions and cross-fertilisation among disciplines, which was one of the aims of the meeting. Moreover, since many stress response mechanisms are broadly conserved, data obtained at the microbial scale may facilitate the comprehension of complex phenomena, such as aging, evolution of neurological diseases and cancer.
[Mh] Termos MeSH primário: Estresse Fisiológico
[Mh] Termos MeSH secundário: Biotecnologia
Microbiologia Industrial
Fenômenos Microbiológicos
Biologia de Sistemas
[Pt] Tipo de publicação:CONGRESSES
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE


  5 / 132 MEDLINE  
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[PMID]:27888322
[Au] Autor:Goswami M; Bhattacharyya P; Tribedi P
[Ad] Endereço:Department of Microbiology, Assam Don Bosco University, Airport Road, Azara, Guwahati, Assam, 781017, India.
[Ti] Título:Allee effect: the story behind the stabilization or extinction of microbial ecosystem.
[So] Source:Arch Microbiol;199(2):185-190, 2017 Mar.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A population exhibiting Allee effect shows a positive correlation between population fitness and population size or density. Allee effect decides the extinction or conservation of a microbial population and thus appears to be an important criterion in population ecology. The underlying factor of Allee effect that decides the stabilization and extinction of a particular population density is the threshold or the critical density of their abundance. According to Allee, microbial populations exhibit a definite, critical or threshold density, beyond which the population fitness of a particular population increases with the rise in population density and below it, the population fitness goes down with the decrease in population density. In particular, microbial population displays advantageous traits such as biofilm formation, expression of virulence genes, spore formation and many more only at a high population density. It has also been observed that microorganisms exhibiting a lower population density undergo complete extinction from the residual microbial ecosystem. In reference to Allee effect, decrease in population density or size introduces deleterious mutations among the population density through genetic drift. Mutations are carried forward to successive generations resulting in its accumulation among the population density thus reducing its microbial fitness and thereby increasing the risk of extinction of a particular microbial population. However, when the microbial load is high, the chance of genetic drift is less, and through the process of biofilm formation, the cooperation existing among the microbial population increases that increases the microbial fitness. Thus, the high microbial population through the formation of microbial biofilm stabilizes the ecosystem by increasing fitness. Taken together, microbial fitness shows positive correlation with the ecosystem conservation and negative correlation with ecosystem extinction.
[Mh] Termos MeSH primário: Ecossistema
Fenômenos Microbiológicos
[Mh] Termos MeSH secundário: Biofilmes
Extinção Biológica
Deriva Genética
Aptidão Genética
Fenômenos Microbiológicos/genética
Modelos Biológicos
Percepção de Quorum
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161127
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-016-1323-4


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[PMID]:27785923
[Au] Autor:den Heijer CD; Geerlings SE; Prins JM; Beerepoot MA; Stobberingh EE; Penders J
[Ad] Endereço:Department of Medical Microbiology, Maastricht University Medical Centre/Care & Public Health Research Institute (CAPHRI), Maastricht, The Netherlands.
[Ti] Título:Can the composition of the intestinal microbiota predict the development of urinary tract infections?
[So] Source:Future Microbiol;11:1395-1404, 2016 Oct.
[Is] ISSN:1746-0921
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: To evaluate whether intestinal microbiota predicts the development of new-onset urinary tract infections (UTIs) in postmenopausal women with prior recurrent UTIs (rUTIs). PATIENTS & METHODS: Fecal samples (n = 40) originated from women with rUTI who received 12 months' prophylaxis of either trimethoprim-sulfamethoxazole (TMP-SMX) or lactobacilli. Microbial composition was assessed by 16S rRNA pyrosequencing. RESULTS: At baseline, fecal microbiota of women with zero and more than or equal to four UTIs during follow-up showed no significant differences. Only TMP-SMX prophylaxis resulted in reduced microbial diversity. Microbial structure of two samples from the same woman showed limited relatedness. CONCLUSION: In postmenopausal women with rUTI, the intestinal microbiota was not predictive for new-onset UTIs. Only TMP-SMX, and not lactobacilli, prophylaxis had effects on the microbial composition. Data in ENA:PRJEB13868.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal/efeitos dos fármacos
Fenômenos Microbiológicos/efeitos dos fármacos
Infecções Urinárias/tratamento farmacológico
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Antibioticoprofilaxia
Biodiversidade
DNA Bacteriano
Fezes/microbiologia
Feminino
Microbioma Gastrointestinal/genética
Seres Humanos
Lactobacillus
Fenômenos Microbiológicos/genética
Meia-Idade
Filogenia
Pós-Menopausa
Probióticos/uso terapêutico
RNA Ribossômico 16S/genética
Fatores de Tempo
Combinação Trimetoprima e Sulfametoxazol/uso terapêutico
Infecções Urinárias/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


  7 / 132 MEDLINE  
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[PMID]:27404249
[Au] Autor:Metcalf JL; Carter DO; Knight R
[Ad] Endereço:Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO 80309, USA; Department of Pediatrics, University of California, San Diego, San Diego, CA 92037, USA. Electronic address: jessica.metcalf@colorado.edu.
[Ti] Título:Microbiology of death.
[So] Source:Curr Biol;26(13):R561-3, 2016 Jul 11.
[Is] ISSN:1879-0445
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Metcalf et al., take a fresh look at the universal question "What happens when we die?"
[Mh] Termos MeSH primário: Osso e Ossos/microbiologia
Morte
Mamíferos/microbiologia
[Mh] Termos MeSH secundário: Animais
Cadáver
Microbiologia Ambiental
Seres Humanos
Fenômenos Microbiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE


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[PMID]:27211839
[Au] Autor:Chichurin AV; Shvychkina HN
[Ad] Endereço:Department of Numerical Analysis and Programming, The John Paul II Catholic University of Lublin, Lublin 20-708, Poland. Electronic address: achichurin@gmail.com.
[Ti] Título:Computer simulation of two chemostat models for one nutrient resource.
[So] Source:Math Biosci;278:30-6, 2016 Aug.
[Is] ISSN:1879-3134
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We consider Michaelis-Menten chemostat dynamic models, describing the process of continuous cultivation of bacteria with one organic substrate and two types of microorganisms in a case where the Michaelis-Menten constants for the two competing species of microorganisms are equal. For such a system we obtain solutions with the finite initial conditions assuming only positive values. As it is shown the problem is reduced to the solution of the nonlinear differential equation of the first order. For some parametric relations the solutions of the differential system are found in the analytical form. Using numerical procedures we construct software modules that allow modeling the chemostat cultivation for the changing parameters and visualizing the dynamics of the development process for each microorganism. A comparative analysis of some numerical methods that are used to integrate the resulting nonlinear differential equation is given.
[Mh] Termos MeSH primário: Simulação por Computador
Ecossistema
Fenômenos Microbiológicos
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160524
[St] Status:MEDLINE


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[PMID]:27190206
[Au] Autor:Fondi M; Karkman A; Tamminen MV; Bosi E; Virta M; Fani R; Alm E; McInerney JO
[Ad] Endereço:Laboratory of Microbial and Molecular Evolution, Department of Biology, University of Florence, Italy Computational Biology Group, University of Florence, Italy.
[Ti] Título:"Every Gene Is Everywhere but the Environment Selects": Global Geolocalization of Gene Sharing in Environmental Samples through Network Analysis.
[So] Source:Genome Biol Evol;8(5):1388-400, 2016 05 13.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The spatial distribution of microbes on our planet is famously formulated in the Baas Becking hypothesis as "everything is everywhere but the environment selects." While this hypothesis does not strictly rule out patterns caused by geographical effects on ecology and historical founder effects, it does propose that the remarkable dispersal potential of microbes leads to distributions generally shaped by environmental factors rather than geographical distance. By constructing sequence similarity networks from uncultured environmental samples, we show that microbial gene pool distributions are not influenced nearly as much by geography as ecology, thus extending the Bass Becking hypothesis from whole organisms to microbial genes. We find that gene pools are shaped by their broad ecological niche (such as sea water, fresh water, host, and airborne). We find that freshwater habitats act as a gene exchange bridge between otherwise disconnected habitats. Finally, certain antibiotic resistance genes deviate from the general trend of habitat specificity by exhibiting a high degree of cross-habitat mobility. The strong cross-habitat mobility of antibiotic resistance genes is a cause for concern and provides a paradigmatic example of the rate by which genes colonize new habitats when new selective forces emerge.
[Mh] Termos MeSH primário: Biodiversidade
Meio Ambiente
Redes Reguladoras de Genes
Determinismo Genético
Especiação Genética
Fenômenos Microbiológicos
[Mh] Termos MeSH secundário: Ecologia
Fluxo Gênico
Geografia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160519
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evw077


  10 / 132 MEDLINE  
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[PMID]:27104979
[Au] Autor:Cao Y; Ryser MD; Payne S; Li B; Rao CV; You L
[Ad] Endereço:Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
[Ti] Título:Collective Space-Sensing Coordinates Pattern Scaling in Engineered Bacteria.
[So] Source:Cell;165(3):620-30, 2016 Apr 21.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Scale invariance refers to the maintenance of a constant ratio of developing organ size to body size. Although common, its underlying mechanisms remain poorly understood. Here, we examined scaling in engineered Escherichia coli that can form self-organized core-ring patterns in colonies. We found that the ring width exhibits perfect scale invariance to the colony size. Our analysis revealed a collective space-sensing mechanism, which entails sequential actions of an integral feedback loop and an incoherent feedforward loop. The integral feedback is implemented by the accumulation of a diffusive chemical produced by a colony. This accumulation, combined with nutrient consumption, sets the timing for ring initiation. The incoherent feedforward is implemented by the opposing effects of the domain size on the rate and duration of ring maturation. This mechanism emphasizes a role of timing control in achieving robust pattern scaling and provides a new perspective in examining the phenomenon in natural systems.
[Mh] Termos MeSH primário: Escherichia coli/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Retroalimentação
Fenômenos Microbiológicos
Modelos Biológicos
Tamanho do Órgão
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE



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