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[PMID]:29182216
[Au] Autor:Viitaniemi L; Abdulmajeed A; Sulaiman T; Söderling E; Närhi T
[Ad] Endereço:Department of Prosthetic Dentistry and Stomatognathic Physiology, University of Turku, FINLAND.
[Ti] Título:Adhesion and Early Colonization of S. Mutans on Lithium Disilicate Reinforced Glass-Ceramics, Monolithic Zirconia and Dual Cure Resin Cement.
[So] Source:Eur J Prosthodont Restor Dent;25(4):228-234, 2017 Dec 01.
[Is] ISSN:0965-7452
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Monolithic zirconia and glass ceramics are increasingly used in implant crowns. Limited data is available on bacterial adhesion and early biofilm formation on these materials. METHODS: Four different materials were investigated: (1) Lithium disilicate glass-ceramics (LDS), (2) Fully stabilized zirconia (FSZ), (3) Partially stabilized zirconia (PSZ), and (4) Dual curing cement (DCC). The materials' surfaces were characterized with spinning disc confocal microscopy and by water contact angle and surface free energy (SFE) measurements. For the adhesion tests the materials were rolled in suspensions of Streptococcus mutans. Early biofilm formation was studied on the materials and allowing the biofilms to form for 24 h. S. mutans cell counts were determined by plate culturing. ANOVA and post-hoc Tukey's tests (p⟨0.05) were used for statistical evaluation. RESULTS: The LDS surfaces were clearly hydrophilic with the highest SFE value (p⟨0.001). For S. mutans adhesion, the ranking of the materials from lowest to highest was: LDS = FSZ ⟨ DCC ⟨ PSZ (p⟨0.05). No significant differences among the materials were noticed in biofilm formation. CONCLUSIONS: LDS has lower S.mutans adhesion than other materials examined in this study, but the difference was not reflected in early biofilm formation.
[Mh] Termos MeSH primário: Aderência Bacteriana
Biofilmes
Cerâmica
Materiais Dentários
Porcelana Dentária
Cimentos de Resina
Streptococcus mutans/isolamento & purificação
Streptococcus mutans/fisiologia
Zircônio
[Mh] Termos MeSH secundário: Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DCR cement); 0 (Dental Materials); 0 (Resin Cements); 0 (lithia disilicate); 12001-21-7 (Dental Porcelain); 85422-94-2 (Glass ceramics); C6V6S92N3C (Zirconium); S38N85C5G0 (zirconium oxide)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1922/EJPRD_01711Viitaniemi07


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[PMID]:29202487
[Au] Autor:Sarabia-Sainz HM; Mata Haro V; Sarabia Sainz JA; Vázquez-Moreno L; Montfort GR
[Ad] Endereço:Laboratorio de Bioquímica de Proteínas y Glicanos Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora 83304, México.
[Ti] Título:Maillard neoglycans as inhibitors for in vitro adhesion of F4 enterotoxigenic Escherichia coli to piglet intestinal cells.
[So] Source:Acta Biochim Pol;64(4):679-686, 2017.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Adhesion of enterotoxigenic (ETEC) E. coli to host intestinal cells is mediated by lectin-like fimbriae that bind to specific glycan moieties on the surfaces of enterocytes. To prevent in vitro binding of E. coli F4 fimbriae (F4 ETEC ) to piglet enterocytes, neoglycans were synthesized by the Maillard reaction conjugating lactose (Lac), galacto-oligosaccharides (GOS) or chitin oligosaccharides (Ochit) to porcine serum albumin (PSA). Neoglycans were characterized by SDS-PAGE, intrinsic tryptophan fluorescence and recognition by plant lectins, as well as by F4 ETEC variants. Electrophoretic patterns suggested the binding to PSA of 63, 13 and 2 molecules of Lac, GOS and Ochit, respectively. All neoglycans displayed quenching of tryptophan fluorescence consistent with the degree of glycation estimated by SDS-PAGE. Plant lectins recognized the neoglycans according to their specificity, whereas antigenic variants of F4 ETEC (ab, ac and ad) recognized PSA-Ochit and PSA-Lac with higher affinity than that for GOS. Neoglycans partially hindered the in vitro binding of F4 ETEC to piglet enterocytes in a dose-dependent manner. The most effective blocking was observed with PSA-Lac that partially inhibited the adhesion of bacteria to enterocytes in a dose dependent manner, as quantified by flow cytometry. Increased production of the cytokines IL-6 and TNF-α was observed in response to F4 ETEC infection of enterocytes and production was reduced in the presence of PSA-Ochit and PSA-GOS. These results suggest that neoglycans synthesized by the Maillard reaction could be useful in the prophylaxis of diarrhea in piglets.
[Mh] Termos MeSH primário: Enterócitos/efeitos dos fármacos
Enterócitos/microbiologia
Escherichia coli Enterotoxigênica/efeitos dos fármacos
Polissacarídeos/química
Polissacarídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Aderência Bacteriana/efeitos dos fármacos
Eletroforese em Gel de Poliacrilamida
Escherichia coli Enterotoxigênica/patogenicidade
Infecções por Escherichia coli/tratamento farmacológico
Infecções por Escherichia coli/microbiologia
Infecções por Escherichia coli/veterinária
Produtos Finais de Glicação Avançada/química
Intestinos/citologia
Intestinos/microbiologia
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Glycation End Products, Advanced); 0 (Polysaccharides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2017_2199


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[PMID]:29315313
[Au] Autor:Obermeier A; Schneider J; Harrasser N; Tübel J; Mühlhofer H; Pförringer D; Deimling CV; Foehr P; Kiefel B; Krämer C; Stemberger A; Schieker M; Burgkart R; von Eisenhart-Rothe R
[Ad] Endereço:Klinik für Orthopädie und Sportorthopädie, Klinikum rechts der Isar der Technischen Universität München, München, Germany.
[Ti] Título:Viable adhered Staphylococcus aureus highly reduced on novel antimicrobial sutures using chlorhexidine and octenidine to avoid surgical site infection (SSI).
[So] Source:PLoS One;13(1):e0190912, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Surgical sutures can promote migration of bacteria and thus start infections. Antiseptic coating of sutures may inhibit proliferation of adhered bacteria and avoid such complications. OBJECTIVES: This study investigated the inhibition of viable adhering bacteria on novel antimicrobially coated surgical sutures using chlorhexidine or octenidine, a critical factor for proliferation at the onset of local infections. The medical need, a rapid eradication of bacteria in wounds, can be fulfilled by a high antimicrobial efficacy during the first days after wound closure. METHODS: As a pretesting on antibacterial efficacy against relevant bacterial pathogens a zone of inhibition assay was conducted with middle ranged concentrated suture coatings (22 µg/cm). For further investigation of adhering bacteria in detail the most clinically relevant Staphylococcus aureus (ATCC®49230™) was used. Absorbable braided sutures were coated with chlorhexidine-laurate, chlorhexidine-palmitate, octenidine-laurate, and octenidine-palmitate. Each coating type resulted in 11, 22, or 33 µg/cm drug content on sutures. Scanning electron microscopy (SEM) was performed once to inspect the coating quality and twice to investigate if bacteria have colonized on sutures. Adhesion experiments were assessed by exposing coated sutures to S. aureus suspensions for 3 h at 37°C. Subsequently, sutures were sonicated and the number of viable bacteria released from the suture surface was determined. Furthermore, the number of viable planktonic bacteria was measured in suspensions containing antimicrobial sutures. Commercially available sutures without drugs (Vicryl®, PGA Resorba®, and Gunze PGA), as well as triclosan-containing Vicryl® Plus were used as control groups. RESULTS: Zone of inhibition assay documented a multispecies efficacy of novel coated sutures against tested bacterial strains, comparable to most relevant S. aureus over 48 hours. SEM pictures demonstrated uniform layers on coated sutures with higher roughness for palmitate coatings and sustaining integrity of coated sutures. Adherent S. aureus were found via SEM on all types of investigated sutures. The novel antimicrobial sutures showed significantly less viable adhered S. aureus bacteria (up to 6.1 log) compared to Vicryl® Plus (0.5 log). Within 11 µg/cm drug-containing sutures, octenidine-palmitate (OL11) showed the highest number of viable adhered S. aureus (0.5 log), similar to Vicryl® Plus. Chlorhexidine-laurate (CL11) showed the lowest number of S. aureus on sutures (1.7 log), a 1.2 log greater reduction. In addition, planktonic S. aureus in suspensions were highly inhibited by CL11 (0.9 log) represents a 0.6 log greater reduction compared to Vicryl® Plus (0.3 log). CONCLUSIONS: Novel antimicrobial sutures can potentially limit surgical site infections caused by multiple pathogenic bacterial species. Therefore, a potential inhibition of multispecies biofilm formation is assumed. In detail tested with S. aureus, the chlorhexidine-laurate coating (CL11) best meets the medical requirements for a fast bacterial eradication. This suture coating shows the lowest survival rate of adhering as well as planktonic bacteria, a high drug release during the first-clinically most relevant- 48 hours, as well as biocompatibility. Thus, CL11 coatings should be recommended for prophylactic antimicrobial sutures as an optimal surgical supplement to reduce wound infections. However, animal and clinical investigations are important to prove safety and efficacy for future applications.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais/administração & dosagem
Aderência Bacteriana
Clorexidina/administração & dosagem
Piridinas/administração & dosagem
Staphylococcus aureus/fisiologia
Infecção da Ferida Cirúrgica/prevenção & controle
Suturas
[Mh] Termos MeSH secundário: Microscopia Eletrônica de Varredura
Infecção da Ferida Cirúrgica/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Pyridines); OZE0372S5A (octenidine); R4KO0DY52L (Chlorhexidine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190912


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[PMID]:28468513
[Au] Autor:Al Ashhab A; Sweity A; Bayramoglu B; Herzberg M; Gillor O
[Ad] Endereço:a Zuckerberg Institute for Water Research, Jacob Blaustein Institutes for Desert Research , Ben-Gurion University of the Negev , Midreshet Ben Gurion , Israel.
[Ti] Título:Biofouling of reverse osmosis membranes: effects of cleaning on biofilm microbial communities, membrane performance, and adherence of extracellular polymeric substances.
[So] Source:Biofouling;33(5):397-409, 2017 05.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Laboratory-scale reverse osmosis (RO) flat-sheet systems were used with two parallel flow cells, one treated with cleaning agents and a control (ie undisturbed). The cleaning efforts increased the affinity of extracellular polymeric substances (EPS) to the RO membrane and altered the biofilm surface structure. Analysis of the membrane biofilm community composition revealed the dominance of Proteobacteria. However, within the phylum Proteobacteria, γ-Proteobacteria dominated the cleaned membrane biofilm, while ß-Proteobacteria dominated the control biofilm. The composition of the fungal phyla was also altered by cleaning, with enhancement of Ascomycota and suppression of Basidiomycota. The results suggest that repeated cleaning cycles select for microbial groups that strongly attach to the RO membrane surface by producing rigid and adhesive EPS that hampers membrane performance.
[Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Incrustação Biológica/prevenção & controle
Detergentes/farmacologia
Membranas Artificiais
Proteobactérias/efeitos dos fármacos
[Mh] Termos MeSH secundário: Ascomicetos/efeitos dos fármacos
Ascomicetos/crescimento & desenvolvimento
Ascomicetos/fisiologia
Filtração
Osmose
Polímeros/química
Proteobactérias/crescimento & desenvolvimento
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Detergents); 0 (Membranes, Artificial); 0 (Polymers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2017.1318382


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[PMID]:29320565
[Au] Autor:Brauge T; Faille C; Sadovskaya I; Charbit A; Benezech T; Shen Y; Loessner MJ; Bautista JR; Midelet-Bourdin G
[Ad] Endereço:ANSES, Laboratory for food safety, Boulogne sur Mer, France.
[Ti] Título:The absence of N-acetylglucosamine in wall teichoic acids of Listeria monocytogenes modifies biofilm architecture and tolerance to rinsing and cleaning procedures.
[So] Source:PLoS One;13(1):e0190879, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The wall teichoic acid (WTA) is the major carbohydrate found within the extracellular matrix of the Listeria monocytogenes biofilm. We first addressed the frequency of spontaneous mutations in two genes (lmo2549 and lmo2550) responsible for the GlcNAcylation in 93 serotype 1/2a strains that were mainly isolated from seafood industries. We studied the impact of mutations in lmo2549 or lmo2550 genes on biofilm formation by using one mutant carrying a natural mutation inactivating the lmo2550 gene (DSS 1130 BFA2 strain) and two EGD-e mutants that lack respective genes by in-frame deletion of lmo2549 or lmo2550 using splicing-by-overlap-extension PCR, followed by allelic exchange mutagenesis. The lmo2550 gene mutation, occurring in around 50% isolates, caused a decrease in bacterial adhesion to stainless steel compared to wild-type EGD-e strain during the adhesion step. On the other hand, bacterial population weren't significantly different after 24h-biofilm formation. The biofilm architecture was different between the wild-type strain and the two mutants inactivated for lmo2549 or lmo2550 genes respectively with the presence of bacterial micro-colonies for mutants which were not observed in the wild-type EGD-e strain biofilm. These differences might account for the stronger hydrophilic surface exhibited by the mutant cells. Upon a water flow or to a cleaning procedure at a shear stress of 0.16 Pa, the mutant biofilms showed the higher detachment rate compared to wild-type strain. Meanwhile, an increase in the amount of residual viable but non-culturable population on stainless steel was recorded in two mutants. Our data suggests that the GlcNAc residue of WTA played a role in adhesion and biofilm formation of Listeria monocyctogenes.
[Mh] Termos MeSH primário: Acetilglucosamina/metabolismo
Aderência Bacteriana/fisiologia
Biofilmes
Parede Celular/metabolismo
Listeria monocytogenes/fisiologia
Ácidos Teicoicos/metabolismo
[Mh] Termos MeSH secundário: Aderência Bacteriana/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Parede Celular/genética
Parede Celular/ultraestrutura
Interações Hidrofóbicas e Hidrofílicas
Listeria monocytogenes/genética
Listeria monocytogenes/ultraestrutura
Microscopia Eletrônica de Transmissão
Mutação
Fenótipo
Aço Inoxidável
Estresse Mecânico
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Teichoic Acids); 059QF0KO0R (Water); 12597-68-1 (Stainless Steel); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190879


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[PMID]:29025667
[Au] Autor:Alvarado-Gomez E; Perez-Diaz M; Valdez-Perez D; Ruiz-Garcia J; Magaña-Aquino M; Martinez-Castañon G; Martinez-Gutierrez F
[Ad] Endereço:Microbiology Laboratory, Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, Av. Manuel Nava No. 6, CP 78210 San Luis Potosi, S.L.P., Mexico.
[Ti] Título:Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:336-344, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S. aureus, other by P. aeruginosa, and a third one by the mix of both strains. With these biofilms, we determined microbial growth by plate counting, and their physicochemical characterization by Atomic Force Microscopy, Raman Micro-Spectroscopy and Scanning Electron Microscopy. We found that the viability of S. aureus was less than P. aeruginosa in multi-species biofilm. However, the adhesion force of S. aureus is much higher than that of P. aeruginosa, but it decreased while that of P. aeruginosa increased in the multi-species biofilm. In addition, we found free pyrimidines functional groups in the P. aeruginosa biofilm and its mix with S. aureus. Surprisingly, each bacterium alone formed single layer biofilms, while the mix bacteria formed a multilayer biofilm at the same observation time. Our results show the necessity to evaluate biofilms from clinically isolated strains and have a better understanding of the adhesion forces of bacteria in biofilm multispecies, which could be of prime importance in developing more effective treatments against biofilm formation.
[Mh] Termos MeSH primário: Aderência Bacteriana/fisiologia
Biofilmes/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Negativas/fisiologia
Bactérias Gram-Positivas/crescimento & desenvolvimento
Bactérias Gram-Positivas/isolamento & purificação
Bactérias Gram-Positivas/fisiologia
Seres Humanos
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Dermatopatias/microbiologia
Dermatopatias/patologia
Análise Espectral Raman
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:28466544
[Au] Autor:Loza-Correa M; Kalab M; Yi QL; Eltringham-Smith LJ; Sheffield WP; Ramirez-Arcos S
[Ad] Endereço:Canadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
[Ti] Título:Comparison of bacterial attachment to platelet bags with and without preconditioning with plasma.
[So] Source:Vox Sang;112(5):401-407, 2017 Jul.
[Is] ISSN:1423-0410
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: Canadian Blood Services produces apheresis and buffy coat pooled platelet concentrates (PCs) stored in bags produced by two different manufacturers (A and B, respectively), both made of polyvinyl chloride-butyryl trihexyl citrate. This study was aimed at comparing Staphylococcus epidermidis adhesion to the inner surface of both bag types in the presence or absence of plasma factors. MATERIALS AND METHODS: Sets (N = 2-6) of bags type A and B were left non-coated (control) or preconditioned with platelet-rich, platelet-poor or defibrinated plasma (PRP, PPP and DefibPPP, respectively). Each bag was inoculated with a 200-ml S. epidermidis culture adjusted to 0·5 colony-forming units/ml. Bags were incubated under platelet storage conditions for 7 days. After culture removal, bacteria attached to the plastic surface were either dislodged by sonication for bacterial quantification or examined in situ by scanning electron microscopy (SEM). RESULTS: Higher bacterial adhesion was observed to preconditioned PC bags than control containers for both bag types (P < 0·0001). Bacterial attachment to preconditioned bags was confirmed by SEM. Bacteria adhered equally to both types of containers in the presence of PRP, PPP and DefibPPP residues (P > 0·05). By contrast, a significant increase in bacterial adherence was observed to type A bags compared with type B bags in the absence of plasma (P < 0·05) [Correction added on 16 June 2017, after first online publication: this sentence has been corrected]. CONCLUSION: The ability of S. epidermidis to adhere to preconditioned platelet collection bags depends on the presence of plasma factors. Future efforts should be focused on reducing plasma proteins' attachment to platelet storage containers to decrease subsequent bacterial adhesion.
[Mh] Termos MeSH primário: Incrustação Biológica/prevenção & controle
Plaquetas
Preservação de Sangue/instrumentação
Staphylococcus epidermidis/fisiologia
[Mh] Termos MeSH secundário: Aderência Bacteriana
Materiais Revestidos Biocompatíveis
Seres Humanos
Plasma/química
Cloreto de Polivinila/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 9002-86-2 (Polyvinyl Chloride)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1111/vox.12513


  8 / 18121 MEDLINE  
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[PMID]:28465066
[Au] Autor:Yoshihara K; Nagaoka N; Maruo Y; Sano H; Yoshida Y; Van Meerbeek B
[Ad] Endereço:Center for Innovative Clinical Medicine, Okayama University Hospital, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
[Ti] Título:Bacterial adhesion not inhibited by ion-releasing bioactive glass filler.
[So] Source:Dent Mater;33(6):723-734, 2017 06.
[Is] ISSN:1879-0097
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Bioactive glasses and surface pre-reacted glass-ionomer (sPRG) filler possess cariostatic properties owing to ion release. Many studies investigated potential cariostatic effects; few studies evaluated the surface stability and the structural changes their surfaces undergo in acidic conditions. METHODS: The surface resistance against acid attack and the surface receptiveness for bacterial adhesion and biofilm formation of a sPRG-filled (Beautifil ll, Shofu) and conventional glass-filled (Herculite XRV Ultra, Kerr) resin-based composite (RBC), and a conventional glass-ionomer cement (GIC; Fuji IX GP Extra, GC) were examined. Specimens (n=3) were immersed in distilled water or lactic acid (pH 4.0) for 3 days. Bacterial growth and biofilm formation were recorded using optical density and SEM. RESULTS: Upon 3-day immersion in lactic acid, the surface of the sPRG-filled RBC revealed multiple holes, while virtually no change in surface integrity was observed for the conventional RBC and GIC. Bacterial growth measurements revealed that none of the materials inhibited Streptococcus mutans (p<0.05). Remarkably, cross-sectional SEM revealed that S. mutans had penetrated the etch pits induced by lactic acid in/around the sPRG filler. Ion-release measurements revealed that sPRG-filled RBC released boron and fluoride, while GIC only released fluoride. However, the concentration of ions released by both materials appeared not sufficient to inhibit bacterial growth. Moreover, the structural surface change and resultant increased surface roughness appeared to have promoted biofilm formation. SIGNIFICANCE: While having bioactive potential through ion release, the stability of surface integrity of bioactive materials is a key-parameter to be assessed with regard to their cariostatic potential.
[Mh] Termos MeSH primário: Aderência Bacteriana
Cariostáticos
Resinas Compostas
Cimentos de Ionômeros de Vidro
[Mh] Termos MeSH secundário: Estudos Transversais
Fluoretos
Teste de Materiais
Streptococcus mutans
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cariostatic Agents); 0 (Composite Resins); 0 (Glass Ionomer Cements); Q80VPU408O (Fluorides)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  9 / 18121 MEDLINE  
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[PMID]:28464847
[Au] Autor:Rajas O; Quirós LM; Ortega M; Vazquez-Espinosa E; Merayo-Lloves J; Vazquez F; García B
[Ad] Endereço:Pneumology Service, Hospital La Princesa, Institute for Health Research (IP), Hospital Universitario de La Princesa, Madrid, Spain.
[Ti] Título:Glycosaminoglycans are involved in bacterial adherence to lung cells.
[So] Source:BMC Infect Dis;17(1):319, 2017 05 02.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lower respiratory infections are among the top ten causes of death worldwide. Since pathogen to cell adhesion is a crucial step in the infection progress, blocking the interaction between eukaryotic receptors and bacterial ligands may enable the pathogenesis process to be stopped. Cell surface glycosaminoglycans (GAGs) are known to be mediators in the adhesion of diverse bacteria to different cell types, making it of interest to examine their involvement in the attachment of various pathogenic bacteria to lung cells, including epithelial cells and fibroblasts. METHODS: The function of cell surface GAGs in bacterial adhesion was studied by reducing their levels through inhibiting their biosynthesis and enzymatic degradation, as well as in binding competition experiments with various species of GAGs. The participation of the different bacterial adhesins in attachment was evaluated through competition with two peptides, both containing consensus heparin binding sequences. Blocking inhibition assays using anti-syndecans and the enzymatic removal of glypicans were conducted to test their involvement in bacterial adhesion. The importance of the fine structure of GAGs in the interaction with pathogens was investigated in competition experiments with specifically desulfated heparins. RESULTS: The binding of all bacteria tested decreased when GAG levels in cell surface of both lung cells were diminished. Competition experiments with different types of GAGs showed that heparan sulfate chains are the main species involved. Blocking or removal of cell surface proteoglycans evidenced that syndecans play a more important role than glypicans. The binding was partially inhibited by peptides including heparin binding sequences. Desulfated heparins also reduced bacterial adhesion to different extents depending on the bacterium and the sulfated residue, especially in fibroblast cells. CONCLUSIONS: Taken together, these data demonstrate that the GAG chains of the cell surface are involved in the adhesion of bacterial adhesins to lung cells. Heparan sulfate seems to be the main species implicated, and binding is dependent on the sulfation pattern of the molecule. These data could facilitate the development of new anti-infective strategies, enabling the development of new procedures for blocking the interaction between pathogens and lung cells more effectively.
[Mh] Termos MeSH primário: Aderência Bacteriana/fisiologia
Glicosaminoglicanos/metabolismo
Bactérias Gram-Positivas/patogenicidade
[Mh] Termos MeSH secundário: Linhagem Celular
Células Epiteliais/microbiologia
Fibroblastos/microbiologia
Heparina/metabolismo
Heparitina Sulfato/metabolismo
Seres Humanos
Pulmão/citologia
Pulmão/microbiologia
Proteoglicanas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycosaminoglycans); 0 (Proteoglycans); 9005-49-6 (Heparin); 9050-30-0 (Heparitin Sulfate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180120
[Lr] Data última revisão:
180120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2418-5


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[PMID]:28745311
[Au] Autor:Chen F; Cui G; Wang S; Nair MKM; He L; Qi X; Han X; Zhang H; Zhang JR; Su J
[Ad] Endereço:Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
[Ti] Título:Outer membrane vesicle-associated lipase FtlA enhances cellular invasion and virulence in Francisella tularensis LVS.
[So] Source:Emerg Microbes Infect;6(7):e66, 2017 Jul 26.
[Is] ISSN:2222-1751
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Francisella tularensis is a highly infectious intracellular pathogen that infects a wide range of host species and causes fatal pneumonic tularemia in humans. ftlA was identified as a potential virulence determinant of the F. tularensis live vaccine strain (LVS) in our previous transposon screen, but its function remained undefined. Here, we show that an unmarked deletion mutant of ftlA was avirulent in a pneumonia mouse model with a severely impaired capacity to infect host cells. Consistent with its sequence homology with GDSL lipase/esterase family proteins, the FtlA protein displayed lipolytic activity in both E. coli and F. tularensis with a preference for relatively short carbon-chain substrates. FtlA thus represents the first F. tularensis lipase to promote bacterial infection of host cells and in vivo fitness. As a cytoplasmic protein, we found that FtlA was secreted into the extracellular environment as a component of outer membrane vesicles (OMVs). Further confocal microscopy analysis revealed that the FtlA-containing OMVs isolated from F. tularensis LVS attached to the host cell membrane. Finally, the OMV-associated FtlA protein complemented the genetic deficiency of the ΔftlA mutant in terms of host cell infection when OMVs purified from the parent strain were co-incubated with the mutant bacteria. These lines of evidence strongly suggest that the FtlA lipase promotes F. tularensis adhesion and internalization by modifying bacterial and/or host molecule(s) when it is secreted as a component of OMVs.
[Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/metabolismo
Francisella tularensis/enzimologia
Francisella tularensis/patogenicidade
Lipase/metabolismo
Macrófagos/microbiologia
[Mh] Termos MeSH secundário: Células A549
Animais
Aderência Bacteriana
Carga Bacteriana
Proteínas da Membrana Bacteriana Externa/química
Proteínas da Membrana Bacteriana Externa/isolamento & purificação
Linhagem Celular
Modelos Animais de Doenças
Células Epiteliais/microbiologia
Escherichia coli/metabolismo
Francisella tularensis/genética
Francisella tularensis/fisiologia
Deleção de Genes
Seres Humanos
Fígado/microbiologia
Pulmão/citologia
Camundongos
Mutação
Células RAW 264.7
Baço/microbiologia
Tularemia/microbiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1038/emi.2017.53



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