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[PMID]:29262709
[Au] Autor:Kaskatepe B; Yildiz SS; Kiymaci ME; Yazgan AN; Cesur S; Erdem SA
[Ad] Endereço:1 Department of Pharmaceutical Microbiology, Ankara University , Ankara , Turkey.
[Ti] Título:Chemical composition and antimicrobial activity of the commercial Origanum onites L. oil against nosocomial carbapenem resistant extended spectrum beta lactamase producer Escherichia coli isolates.
[So] Source:Acta Biol Hung;68(4):466-476, 2017 Dec.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:In recent years rapidly growing antibiotic resistance has increased interest toward natural products, especially essential oils because of their various effects. The aim of this study was to identify the chemical composition of the commercial Origanum onites essential oil (EO) and to investigate the antimicrobial activity by disc diffusion and dilution methods, against ten different ATCC strains, including eight bacteria, two yeasts and seventy-nine clinical nosocomial Escherichia coli isolates that produce extended spectrum beta lactamase (ESBL). The chemical composition of EO was analyzed by GC and GC-MS. The major compounds of the EO were determined as carvacrol (51.4%) followed by linalool (11.2%), p-cymene (8.9%) and γ-terpinene (6.7%). O. onites EO had antimicrobial activity against all standard strains and inhibited microbial growth of ESBL positive E. coli isolates. According to our results, O. onites EO may be an alternative to synthetic drug, used in combination with other antibiotics for treatment of infection caused by multidrug resistant bacteria after testing toxic effects and irritation at preferred doses on human.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Carbapenêmicos
Proteínas de Escherichia coli/metabolismo
Escherichia coli/crescimento & desenvolvimento
Óleos Voláteis/farmacologia
Origanum/química
Resistência beta-Lactâmica/efeitos dos fármacos
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Anti-Infecciosos/química
Óleos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Carbapenems); 0 (Escherichia coli Proteins); 0 (Oils, Volatile); 39736Z7Q8W (origanum oil); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.4.11


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[PMID]:28449953
[Au] Autor:Rezende TFT; Doi AM; Quiles MG; Pignatari ACC; Manfrendi S; Grothe C; Taminato M; Barbosa DA
[Ad] Endereço:Special Clinical Microbiology Laboratory (LEMC/ALERTA), Federal University of São Paulo/UNIFESP/Brazil, São Paulo, Brazil. Electronic address: thaisftr_@hotmail.com.
[Ti] Título:Detection of colonization by carbapenem-resistant organisms by real-time polymerase chain reaction from rectal swabs in patients with chronic renal disease.
[So] Source:J Hosp Infect;96(2):123-128, 2017 Jun.
[Is] ISSN:1532-2939
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Carbapenem-resistant organism (CRO) colonization is a serious problem that increases the risk of infection and contributes to dissemination of antimicrobial resistance in healthcare-associated environments. The risk of acquisition and dissemination of CRO is high in chronic renal failure patients and the surveillance culture is recommended as a component of infection control programmes. AIM: To assess colonization by CRO, comparing phenotypic and molecular-based methods of diagnostics, in rectal swabs in a large population of chronic renal failure patients. METHODS: A total of 1092 rectal swabs (ESwab™) were collected at two different times from 546 chronic kidney disease (CKD) patients from a specialized tertiary care university centre. They were divided into three groups: conservative treatment (N = 129), dialysis (N = 217), and transplanted patients (N = 200). A chromogenic (CHROMagar™) KPC agar and the multiplex real-time polymerase chain reaction (qPCR) targeting carbapenemase-encoding genes were tested as phenotypic and molecular screening for carbapenemase production. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and conventional PCR were also performed on the isolates grown on chromogenic agar. FINDINGS: Among the 1092 samples, 150 (13.7%) were identified as CRO producers according to chromogenic agar. Only 26 (2.4%) were confirmed as KPC by conventional PCR. According to qPCR direct from swab, 31 (2.8%) were positive for KPC, 39 (3.6%) for GES, and three (0.3%) for SPM with kappa index of 0.256. CONCLUSION: The qPCR technique provides faster results when compared to culture method and enables rapid implementation of control measures and interventions to reduce the spread of CRO in healthcare settings, especially among CKD patients.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Bactérias Gram-Negativas/isolamento & purificação
Infecções por Bactérias Gram-Negativas/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reto/microbiologia
Insuficiência Renal Crônica/complicações
Resistência beta-Lactâmica
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/análise
Técnicas Bacteriológicas/métodos
Portador Sadio/diagnóstico
Portador Sadio/microbiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Hospitais Universitários
Seres Humanos
Técnicas de Diagnóstico Molecular/métodos
Fatores de Tempo
beta-Lactamases/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:29195767
[Au] Autor:Lü Y; Liu W; Liang H; Zhao S; Zhang W; Liu J; Jin C; Hu H
[Ad] Endereço:State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
[Ti] Título:NDM-1 encoded by a pNDM-HN380-like plasmid pNDM-BJ03 in clinical Enterobacter cloacae.
[So] Source:Diagn Microbiol Infect Dis;90(2):153-155, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A carbapenemase-producing Enterobacter cloacae hhy03 with a bla and bla -coharboring plasmid was isolated from a sputum specimen of a patient. This is the third nucleotide sequence report of bla -harboring plasmid from Enterobacter cloacae that have caused lethal infections in China, indicating the spread of NDM-1 by IncX3 plasmid between Enterobacteriaceae.
[Mh] Termos MeSH primário: Enterobacter cloacae/efeitos dos fármacos
Enterobacter cloacae/genética
Infecções por Enterobacteriaceae/microbiologia
Plasmídeos/genética
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
China
Enterobacter cloacae/enzimologia
Seres Humanos
Lactente
Klebsiella pneumoniae/genética
Masculino
Testes de Sensibilidade Microbiana
Escarro/microbiologia
Resistência beta-Lactâmica/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase NDM-1); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


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[PMID]:29174734
[Au] Autor:Kenna DTD; Fuller A; Martin K; Perry C; Pike R; Burns PJ; Narayan O; Wilkinson S; Hill R; Woodford N; Logan JMJ; Turton JF
[Ad] Endereço:Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK. Electronic address: dervla.kenna@phe.gov.uk.
[Ti] Título:rpoB gene sequencing highlights the prevalence of an E. miricola cluster over other Elizabethkingia species among UK cystic fibrosis patients.
[So] Source:Diagn Microbiol Infect Dis;90(2):109-114, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Difficulties in distinguishing species of the Elizabethkingia genus by MALDI-TOF prompted use of rpoB sequencing to investigate species distribution among 44 isolates from cystic fibrosis (CF) patients. Forty-three isolates from 38 patients formed a cluster comprising E. miricola and proposed novel species E. bruuniana sp. nov., the exception clustering with proposed species E. ursingii sp. nov., also part of this wider cluster. All 44 isolates were PCR-positive for urease gene ureG, whereas only one of 23 E. anophelis isolates from non-CF patients was positive, suggesting that this gene is largely associated with the E. miricola cluster. Antibiotic susceptibilities of 12 CF isolates revealed all were resistant to beta-lactams with the exception of piperacillin-tazobactam, and were only susceptible to minocycline and co-trimoxazole. Pulsed-field gel electrophoresis analysis revealed 4 shared strains among 17 CF patients in one pediatric clinic, but epidemiological investigations did not support patient-to-patient transmission except between one sibling pair.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Proteínas de Transporte/genética
Fibrose Cística/microbiologia
RNA Polimerases Dirigidas por DNA/genética
Infecções por Flavobacteriaceae/microbiologia
Flavobacteriaceae/genética
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Feminino
Flavobacteriaceae/classificação
Flavobacteriaceae/efeitos dos fármacos
Infecções por Flavobacteriaceae/epidemiologia
Seres Humanos
Lactente
Masculino
Testes de Sensibilidade Microbiana
Epidemiologia Molecular
Tipagem Molecular
Prevalência
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Reino Unido/epidemiologia
Resistência beta-Lactâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (ureG protein, Bacteria); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29174735
[Au] Autor:Shalom O; Adler A
[Ad] Endereço:Clinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Israel.
[Ti] Título:Comparative study of 3 carbapenem-hydrolysis methods for the confirmation of carbapenemase production in Enterobacteriaceae.
[So] Source:Diagn Microbiol Infect Dis;90(2):73-76, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The goals of the study were to examine the analytic and performance parameters of 2 commercial rapid carbapenem-hydrolysis assays, the ß-CARBA test (Bio-Rad) and the Rapid CARB Blue Kit (ROSCO) in comparison with an in-house CARBA NP assay for the detection of Carbapenemase-Producing Enterobacteriaceae (CPE). Their performance was evaluated following growth on 2 chromogenic CPE screening media. The sensitivity was highest (91%) in the ß-CARBA test when used from the mSuperCARBA™ plates and was lowest when the same test was used from the chromID™ CARBA plates (75%). The specificity was highest in the NP CARBA test in both media (96%), followed by the ß CARBA/mSuperCARBA™ combination (92%). The specificity of the Rapid CARB Blue Kit was as low as 36% when used with the chromID™ CARBA plates. The ß-CARBA test was simple to use and had the shortest turn-around time and hand-on time.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Proteínas de Bactérias/metabolismo
Técnicas de Tipagem Bacteriana/métodos
Carbapenêmicos/metabolismo
Enterobacteriaceae/enzimologia
Resistência beta-Lactâmica
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/análise
Técnicas de Tipagem Bacteriana/normas
Enterobacteriaceae/metabolismo
Infecções por Enterobacteriaceae/microbiologia
Seres Humanos
Hidrólise
Sensibilidade e Especificidade
beta-Lactamases/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carbapenems); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:27773498
[Au] Autor:Lenhard JR; Gall JS; Bulitta JB; Thamlikitkul V; Landersdorfer CB; Forrest A; Nation RL; Li J; Tsuji BT
[Ad] Endereço:Laboratory for Antimicrobial Pharmacodynamics, NYS Center of Excellence in Bioinformatics & Life Sciences, Buffalo, NY, USA; School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, NY, USA; California Northstate University College of Pharmacy.
[Ti] Título:Comparative pharmacodynamics of four different carbapenems in combination with polymyxin B against carbapenem-resistant Acinetobacter baumannii.
[So] Source:Int J Antimicrob Agents;48(6):719-724, 2016 Dec.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to determine the comparative pharmacodynamics of four different carbapenems in combination with polymyxin B (PMB) against carbapenem-resistant Acinetobacter baumannii isolates using time-kill experiments at two different inocula. Two A. baumannii strains (03-149-1 and N16870) with carbapenem minimum inhibitory concentrations (MICs) ranging from 8 to 64 mg/L were investigated in 48-h time-kill experiments using starting inocula of 10 CFU/mL and 10 CFU/mL. Concentration arrays of ertapenem, doripenem, meropenem and imipenem at 0.25×, 0.5×, 1×, 1.5× and 2× published maximum serum concentration (C ) values (C concentrations of 12, 21, 48 and 60 mg/L, respectively) were investigated in the presence of 1.5 mg/L PMB. Use of carbapenems without PMB resulted in drastic re-growth. All carbapenem combinations were able to achieve a ≥3 log CFU/mL reduction by 4 h against both strains at 10 CFU/mL, whereas maximum reductions against strain 03-149-1 at 10 CFU/mL were 1.0, 3.2, 2.2 and 3.3 log CFU/mL for ertapenem, doripenem, meropenem and imipenem, respectively. None of the combinations were capable of reducing 10 CFU/mL of N16870 by ≥2 log CFU/mL. Ertapenem combinations consistently displayed the least activity, whereas doripenem, meropenem and imipenem combinations had similar activities that were poorly predicted by carbapenem MICs. As doripenem, meropenem, or imipenem displayed similar pharmacodyanmics in combination, the decision of which carbapenem to use in combination with PMB may be based on toxicodynamic profiles if drastic discordance in MICs is not present.
[Mh] Termos MeSH primário: Acinetobacter baumannii/efeitos dos fármacos
Antibacterianos/farmacologia
Carbapenêmicos/farmacologia
Interações Medicamentosas
Polimixina B/farmacologia
Resistência beta-Lactâmica
[Mh] Termos MeSH secundário: Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems); 1404-26-8 (Polymyxin B)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28457352
[Au] Autor:Iovleva A; Doi Y
[Ad] Endereço:Division of Infectious Diseases, University of Pittsburgh School of Medicine, Falk Medical Building, Suite 3A, 3601 Fifth Avenue, Pittsburgh, PA 15213, USA.
[Ti] Título:Carbapenem-Resistant Enterobacteriaceae.
[So] Source:Clin Lab Med;37(2):303-315, 2017 Jun.
[Is] ISSN:1557-9832
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a major threat. Commonly used antibiotics are generally inactive against CRE. Therefore, timely detection of CRE is of paramount importance. Among CRE, those producing carbapenem-hydrolyzing ß-lactamase enzymes (carbapenemase-producing Enterobacteriaceae) are particularly of concern because they tend to spread, and treatment is difficult. The carbapenemase groups most commonly encountered include KPC, NDM, and OXA-48. Treatment options are limited and include combinations of polymyxins, tigecycline, aminoglycosides, or carbapenems; newer agents with activity against CRE and better safety profiles are becoming available and will likely emerge as the preferred therapy.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Carbapenêmicos/uso terapêutico
Enterobacteriaceae/efeitos dos fármacos
Resistência beta-Lactâmica
[Mh] Termos MeSH secundário: Enterobacteriaceae/enzimologia
Testes de Sensibilidade Microbiana
beta-Lactamases/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28910381
[Au] Autor:Sugawara Y; Akeda Y; Sakamoto N; Takeuchi D; Motooka D; Nakamura S; Hagiya H; Yamamoto N; Nishi I; Yoshida H; Okada K; Zin KN; Aye MM; Tonomo K; Hamada S
[Ad] Endereço:Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
[Ti] Título:Genetic characterization of blaNDM-harboring plasmids in carbapenem-resistant Escherichia coli from Myanmar.
[So] Source:PLoS One;12(9):e0184720, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bacterial enzyme New Delhi metallo-ß-lactamase hydrolyzes almost all ß-lactam antibiotics, including carbapenems, which are drugs of last resort for severe bacterial infections. The spread of carbapenem-resistant Enterobacteriaceae that carry the New Delhi metallo-ß-lactamase gene, blaNDM, poses a serious threat to public health. In this study, we genetically characterized eight carbapenem-resistant Escherichia coli isolates from a tertiary care hospital in Yangon, Myanmar. The eight isolates belonged to five multilocus-sequence types and harbored multiple antimicrobial-resistance genes, resulting in resistance against nearly all of the antimicrobial agents tested, except colistin and fosfomycin. Nine plasmids harboring blaNDM genes were identified from these isolates. Multiple blaNDM genes were found in the distinct Inc-replicon types of the following plasmids: an IncA/C2 plasmid harboring blaNDM-1 (n = 1), IncX3 plasmids harboring blaNDM-4 (n = 2) or blaNDM-7 (n = 1), IncFII plasmids harboring blaNDM-4 (n = 1) or blaNDM-5 (n = 3), and a multireplicon F plasmid harboring blaNDM-5 (n = 1). Comparative analysis highlighted the diversity of the blaNDM-harboring plasmids and their distinct characteristics, which depended on plasmid replicon types. The results indicate circulation of phylogenetically distinct strains of carbapenem-resistant E. coli with various plasmids harboring blaNDM genes in the hospital.
[Mh] Termos MeSH primário: Infecções por Escherichia coli/microbiologia
Escherichia coli/classificação
Plasmídeos/genética
Resistência beta-Lactâmica
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Carbapenêmicos/farmacologia
DNA Bacteriano/genética
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Proteínas de Escherichia coli/genética
Evolução Molecular
Seres Humanos
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
Mianmar
Filogenia
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbapenems); 0 (DNA, Bacterial); 0 (Escherichia coli Proteins); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase NDM-1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184720


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[PMID]:28877757
[Au] Autor:Runcharoen C; Raven KE; Reuter S; Kallonen T; Paksanont S; Thammachote J; Anun S; Blane B; Parkhill J; Peacock SJ; Chantratita N
[Ad] Endereço:Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok, 10400, Thailand.
[Ti] Título:Whole genome sequencing of ESBL-producing Escherichia coli isolated from patients, farm waste and canals in Thailand.
[So] Source:Genome Med;9(1):81, 2017 Sep 06.
[Is] ISSN:1756-994X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tackling multidrug-resistant Escherichia coli requires evidence from One Health studies that capture numerous potential reservoirs in circumscribed geographic areas. METHODS: We conducted a survey of extended ß-lactamase (ESBL)-producing E. coli isolated from patients, canals and livestock wastewater in eastern Thailand between 2014 and 2015, and analyzed isolates using whole genome sequencing. RESULTS: The bacterial collection of 149 isolates consisted of 84 isolates from a single hospital and 65 from the hospital sewer, canals and farm wastewater within a 20 km radius. E. coli ST131 predominated the clinical collection (28.6%), but was uncommon in the environment. Genome-based comparison of E. coli from infected patients and their immediate environment indicated low genetic similarity overall between the two, although three clinical-environmental isolate pairs differed by ≤ 5 single nucleotide polymorphisms. Thai E. coli isolates were dispersed throughout a phylogenetic tree containing a global E. coli collection. All Thai ESBL-positive E. coli isolates were multidrug resistant, including high rates of resistance to tobramycin (77.2%), gentamicin (77.2%), ciprofloxacin (67.8%) and trimethoprim (68.5%). ESBL was encoded by six different CTX-M elements and SHV-12. Three isolates from clinical samples (n = 2) or a hospital sewer (n = 1) were resistant to the carbapenem drugs (encoded by NDM-1, NDM-5 or GES-5), and three isolates (clinical (n = 1) and canal water (n = 2)) were resistant to colistin (encoded by mcr-1); no isolates were resistant to both carbapenems and colistin. CONCLUSIONS: Tackling ESBL-producing E. coli in this setting will be challenging based on widespread distribution, but the low prevalence of resistance to carbapenems and colistin suggests that efforts are now required to prevent these from becoming ubiquitous.
[Mh] Termos MeSH primário: Resistência a Múltiplos Medicamentos
Infecções por Escherichia coli/microbiologia
Escherichia coli/isolamento & purificação
Águas Residuais/microbiologia
Resistência beta-Lactâmica
[Mh] Termos MeSH secundário: Escherichia coli/enzimologia
Escherichia coli/genética
Escherichia coli/fisiologia
Infecções por Escherichia coli/fisiopatologia
Fazendas
Hospitais
Seres Humanos
Análise de Sequência de DNA
Tailândia
beta-Lactamases
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1186/s13073-017-0471-8


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[PMID]:28784300
[Au] Autor:Zhang J; Wang S; Wei Q; Guo Q; Bai Y; Yang S; Song F; Zhang L; Lei X
[Ad] Endereço:Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for
[Ti] Título:Synthesis and biological evaluation of Aspergillomarasmine A derivatives as novel NDM-1 inhibitor to overcome antibiotics resistance.
[So] Source:Bioorg Med Chem;25(19):5133-5141, 2017 Oct 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ß-lactam antibiotic resistance of Gram-negative bacteria has shown to be a critical global health problem. One of the primary reasons for the drug resistance is the existence of ß-lactamases especially metallo-ß-lactamases such as New Delhi metallo-ß-lactamase (NDM-1) and Verona Integron-encoded metallo-ß-lactamase (VIM-2). The fungal natural product Aspergillomarasmine A (AMA) has proven to be a promising inhibitor of NDM-1 and VIM-2 both in vitro and in vivo. Seven new analogues of AMA were synthesized by utilizing different strategies. The biological evaluation of these analogues was performed to study the structure-activity relationship of AMA both in vitro and in vivo. Remarkably, the lead compound 4 showed synergistic effect in combination with Meropenem to overcome the antibiotic resistance of the Gram-negative bacteria such as K. pneumoniae (BAA-2146) expressing NDM-1.
[Mh] Termos MeSH primário: Ácido Aspártico/análogos & derivados
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/enzimologia
Inibidores de beta-Lactamases/química
Inibidores de beta-Lactamases/farmacologia
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Ácido Aspártico/síntese química
Ácido Aspártico/química
Ácido Aspártico/farmacologia
Seres Humanos
Infecções por Klebsiella/tratamento farmacológico
Infecções por Klebsiella/microbiologia
Resistência beta-Lactâmica/efeitos dos fármacos
Inibidores de beta-Lactamases/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (beta-Lactamase Inhibitors); 30KYC7MIAI (Aspartic Acid); 3484-65-9 (aspergillomarasmine A); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase NDM-1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE



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