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Pesquisa : G06.099.225.750 [Categoria DeCS]
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[PMID]:28390239
[Au] Autor:Guo N; Wang Y; Yan L; Wang X; Wang M; Xu H; Wang S
[Ad] Endereço:Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
[Ti] Título:Effect of bio-electrochemical system on the fate and proliferation of chloramphenicol resistance genes during the treatment of chloramphenicol wastewater.
[So] Source:Water Res;117:95-101, 2017 Jun 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bioelectrochemical systems can effectively degrade antibiotics, but there is the need to better understand the fate of antibiotic resistance bacteria and antibiotic resistance genes during the bioelectrochemical degradation of antibiotics. In this study, a BES was developed as a platform to investigate the fate of chloramphenicol resistance bacteria (CRB) and the expression of chloramphenicol resistance genes (CRGs) under different operating conditions during chloramphenicol biodegradation. The results indicated that chloramphenicol was effectively removed and chloramphenicol removal efficiency could be improved under less chloramphenicol concentration and more negative cathode potential. Higher chloramphenicol concentration enhanced the enrichment of CRB and expression of CRGs. Furthermore, the abundances of CRB were enhanced under more negative cathode potential, the expression of CRGs under less negative cathode potential were induced. However, both the enrichment of CRB and expression of CRGs could be moderated under a medium cathode potential. This result could provide the scientific reference for research about the fate of antibiotic resistance genes in bioelectrochemical systems.
[Mh] Termos MeSH primário: Cloranfenicol
Águas Residuais
[Mh] Termos MeSH secundário: Resistência ao Cloranfenicol
Técnicas Eletroquímicas
Eletrodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); 66974FR9Q1 (Chloramphenicol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE


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[PMID]:26715590
[Au] Autor:Zienkiewicz M; Krupnik T; Drozak A; Golke A; Romanowska E
[Ad] Endereço:Warsaw University, Warsaw, Poland. maximus@biol.uw.edu.pl.
[Ti] Título:Chloramphenicol acetyltransferase-a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae.
[So] Source:Protoplasma;254(1):587-596, 2017 Jan.
[Is] ISSN:1615-6102
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondrial and plastid physiology as well as various evolutionary, structural, and functional features of the photosynthetic apparatus.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Cloranfenicol O-Acetiltransferase/metabolismo
Resistência ao Cloranfenicol/genética
Rodófitas/genética
Transformação Genética
[Mh] Termos MeSH secundário: Marcadores Genéticos
Mutação/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151231
[St] Status:MEDLINE
[do] DOI:10.1007/s00709-015-0936-9


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[PMID]:26932362
[Au] Autor:Hoffmann SA; Kruse SM; Arndt KM
[Ad] Endereço:Molecular Biotechnology, Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.
[Ti] Título:Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches.
[So] Source:Nucleic Acids Res;44(10):e95, 2016 Jun 02.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We have investigated transcriptional interference between convergent genes in E. coli and demonstrate substantial interference for inter-promoter distances of as far as 3 kb. Interference can be elicited by both strong σ(70) dependent and T7 promoters. In the presented design, a strong promoter driving gene expression of a 'forward' gene interferes with the expression of a 'reverse' gene by a weak promoter. This arrangement allows inversely correlated gene expression without requiring further regulatory components. Thus, modulation of the activity of the strong promoter alters expression of both the forward and the reverse gene. We used this design to develop a dual selection system for conditional operator site binding, allowing positive selection both for binding and for non-binding to DNA. This study demonstrates the utility of this novel system using the Lac repressor as a model protein for conditional DNA binding, and spectinomycin and chloramphenicol resistance genes as positive selection markers in liquid culture. Randomized LacI libraries were created and subjected to subsequent dual selection, but mispairing IPTG and selection cues in respect to the wild-type LacI response, allowing the isolation of a LacI variant with a reversed IPTG response within three rounds of library generation and dual selection.
[Mh] Termos MeSH primário: Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Engenharia Genética/métodos
Seleção Genética
[Mh] Termos MeSH secundário: Cloranfenicol/farmacologia
Resistência ao Cloranfenicol/genética
RNA Polimerases Dirigidas por DNA/genética
RNA Polimerases Dirigidas por DNA/metabolismo
Farmacorresistência Bacteriana/genética
Proteínas de Escherichia coli/genética
Óperon Lac
Repressores Lac/genética
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Regiões Operadoras Genéticas
Regiões Promotoras Genéticas
Espectinomicina/farmacologia
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Lac Repressors); 0 (LacI protein, E coli); 0 (Luminescent Proteins); 0 (Viral Proteins); 0 (blue fluorescent protein, Aequorea victoria); 66974FR9Q1 (Chloramphenicol); 93AKI1U6QF (Spectinomycin); EC 2.7.7.- (bacteriophage T7 RNA polymerase); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160303
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkw125


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[PMID]:26552396
[Au] Autor:Dubert J; Osorio CR; Prado S; Barja JL
[Ad] Endereço:Department of Microbiology and Parasitology, CIBUS-Faculty of Biology and Aquaculture Institute, University of Santiago de Compostela, Santiago de Compostela, 15782, Spain. javier.dubert@usc.es.
[Ti] Título:Persistence of Antibiotic Resistant Vibrio spp. in Shellfish Hatchery Environment.
[So] Source:Microb Ecol;72(4):851-860, 2016 11.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The characterization of antibiotic-resistant vibrios isolated from shellfish aquaculture is necessary to elucidate the potential transfer of resistance and to establish effective strategies against vibriosis. With this aim, we analyzed a collection of bacterial isolates obtained from 15 failed hatchery larval cultures that, for the most part, had been treated experimentally with chloramphenicol to prevent vibriosis. Isolates were obtained during a 2-year study from experimental cultures of five different clam species. Among a total of 121 Vibrio isolates studied, 28 were found to be chloramphenicol resistant, suggesting that the shellfish hatchery had been using a sublethal concentration of the antibiotic. Interestingly, chloramphenicol-resistant vibrios showed also resistance to tetracycline and amoxicillin (group A; n = 19) or to streptomycin (group B; n = 9). Chloramphenicol-resistant vibrios were subjected to a PCR amplification and DNA sequencing of the chloramphenicol acetyltransferase genes (cat), and the same approach was followed to study the tetracycline resistance markers (tet). 16S ribosomal RNA (rRNA) gene sequencing revealed that chloramphenicol-resistant vibrios pertained mostly to the Splendidus clade. Conjugation assays demonstrated that various R-plasmids which harbored the cat II/tet(D) genes and cat III gene in groups A and B respectively, were transferred to E. coli and bivalve pathogenic vibrios. Most interestingly, transconjugants exhibited the antibiotic resistance patterns of the donors, despite having been selected only on the basis of chloramphenicol resistance. This is the first report carried out in a bivalve hatchery elucidating the persistence of resistant vibrios, the mechanisms of antibiotic resistance, and the transfer of different R-plasmids.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bivalves/microbiologia
Resistência ao Cloranfenicol/genética
Farmacorresistência Bacteriana Múltipla/genética
Escherichia coli/genética
Pesqueiros
Frutos do Mar/microbiologia
Vibrio/genética
[Mh] Termos MeSH secundário: Amoxicilina/farmacologia
Animais
Sequência de Bases
Cloranfenicol/farmacologia
Cloranfenicol O-Acetiltransferase/genética
DNA Bacteriano/genética
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade Microbiana
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Estreptomicina/farmacologia
Tetraciclina/farmacologia
Vibrio/efeitos dos fármacos
Vibrio/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 66974FR9Q1 (Chloramphenicol); 804826J2HU (Amoxicillin); EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase); F8VB5M810T (Tetracycline); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151111
[St] Status:MEDLINE


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Zanella, Rosemeire Cobo
Brandileone, Maria Cristina de Cunto
Brandäo, Angela Pires
PubMed Central Texto completo
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[PMID]:26517654
[Au] Autor:Zanella RC; Brandileone MC; Andrade AL; Ogassavara CT; Fiório CE; Brandão AP; Almeida SC; Lemos AP; Gorla MC; Carvalhanas TR; Sato H; Liphaus B; Nerger ML; Conde M; Ribeiro AF
[Ad] Endereço:Centro de Bacteriologia, Instituto Adolfo Lutz, Secretaria de Estado da Saúde de São Paulo, São Paulo, SP, Brasil.
[Ti] Título:Evaluation of Haemophilus influenzae type b carrier status among children 10 years after the introduction of Hib vaccine in Brazil.
[So] Source:Mem Inst Oswaldo Cruz;110(6):755-9, 2015 Sep.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of the present study was to assess the prevalence of Haemophilus influenzae type b (Hib) nasopharyngeal (NP) colonisation among healthy children where Hib vaccination using a 3p+0 dosing schedule has been routinely administered for 10 years with sustained coverage (> 90%). NP swabs were collected from 2,558 children who had received the Hib vaccine, of whom 1,379 were 12-< 24 months (m) old and 1,179 were 48-< 60 m old. Hi strains were identified by molecular methods. Hi carriage prevalence was 45.1% (1,153/2,558) and the prevalence in the 12-< 24 m and 48-< 60 m age groups were 37.5% (517/1,379) and 53.9% (636/1,179), respectively. Hib was identified in 0.6% (16/2,558) of all children in the study, being 0.8% (11/1,379) and 0.4% (5/1,179) among the 12-< 24 m and 48-< 60 m age groups, respectively. The nonencapsulate Hi colonisation was 43% (n = 1,099) and was significantly more frequent at 48-< 60 m of age (51.6%, n = 608) compared with that at 12-< 24 m of age (35.6%, n = 491). The overall resistance rates to ampicillin and chloramphenicol were 16.5% and 3.7%, respectively; the co-resistance was detected in 2.6%. Our findings showed that the Hib carrier rate in healthy children under five years was very low after 10 years of the introduction of the Hib vaccine.
[Mh] Termos MeSH primário: Portador Sadio/imunologia
Infecções por Haemophilus/prevenção & controle
Vacinas Anti-Haemophilus/uso terapêutico
Haemophilus influenzae tipo b/imunologia
Nasofaringe/microbiologia
[Mh] Termos MeSH secundário: Resistência a Ampicilina/imunologia
Cápsulas Bacterianas/imunologia
Brasil/epidemiologia
Portador Sadio/microbiologia
Pré-Escolar
Resistência ao Cloranfenicol/imunologia
Estudos Transversais
Infecções por Haemophilus/epidemiologia
Haemophilus influenzae tipo b/classificação
Seres Humanos
Esquemas de Imunização
Lactente
Vacinação em Massa
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Prevalência
Inquéritos e Questionários
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Haemophilus Vaccines)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:151204
[Lr] Data última revisão:
151204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151031
[St] Status:MEDLINE


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[PMID]:26319877
[Au] Autor:Kobayashi J; Tanabiki M; Doi S; Kondo A; Ohshiro T; Suzuki H
[Ad] Endereço:Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
[Ti] Título:Unique plasmids generated via pUC replicon mutagenesis in an error-prone thermophile derived from Geobacillus kaustophilus HTA426.
[So] Source:Appl Environ Microbiol;81(21):7625-32, 2015 Nov.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The plasmid pGKE75-catA138T, which comprises pUC18 and the catA138T gene encoding thermostable chloramphenicol acetyltransferase with an A138T amino acid replacement (CATA138T), serves as an Escherichia coli-Geobacillus kaustophilus shuttle plasmid that confers moderate chloramphenicol resistance on G. kaustophilus HTA426. The present study examined the thermoadaptation-directed mutagenesis of pGKE75-catA138T in an error-prone thermophile, generating the mutant plasmid pGKE75(αß)-catA138T responsible for substantial chloramphenicol resistance at 65°C. pGKE75(αß)-catA138T contained no mutation in the catA138T gene but had two mutations in the pUC replicon, even though the replicon has no apparent role in G. kaustophilus. Biochemical characterization suggested that the efficient chloramphenicol resistance conferred by pGKE75(αß)-catA138T is attributable to increases in intracellular CATA138T and acetyl-coenzyme A following a decrease in incomplete forms of pGKE75(αß)-catA138T. The decrease in incomplete plasmids may be due to optimization of plasmid replication by RNA species transcribed from the mutant pUC replicon, which were actually produced in G. kaustophilus. It is noteworthy that G. kaustophilus was transformed with pGKE75(αß)-catA138T using chloramphenicol selection at 60°C. In addition, a pUC18 derivative with the two mutations propagated in E. coli at a high copy number independently of the culture temperature and high plasmid stability. Since these properties have not been observed in known plasmids, the outcomes extend the genetic toolboxes for G. kaustophilus and E. coli.
[Mh] Termos MeSH primário: Resistência ao Cloranfenicol
Escherichia coli/genética
Vetores Genéticos
Geobacillus/genética
Mutagênese
Mutação
Plasmídeos
[Mh] Termos MeSH secundário: Acetilcoenzima A/metabolismo
Cloranfenicol O-Acetiltransferase/biossíntese
Temperatura Alta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
72-89-9 (Acetyl Coenzyme A); EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150831
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01574-15


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[PMID]:26049592
[Au] Autor:Bossé JT; Li Y; Atherton TG; Walker S; Williamson SM; Rogers J; Chaudhuri RR; Weinert LA; Holden MT; Maskell DJ; Tucker AW; Wren BW; Rycroft AN; Langford PR; BRaDP1T consortium
[Ad] Endereço:Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, W2 1PG, UK. Electronic address: j.bosse@imperial.ac.uk.
[Ti] Título:Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae.
[So] Source:Vet Microbiol;178(3-4):279-82, 2015 Aug 05.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/microbiologia
Actinobacillus pleuropneumoniae/efeitos dos fármacos
Farmacorresistência Bacteriana/genética
Infecções por Pasteurellaceae/microbiologia
Pasteurellaceae/genética
Plasmídeos/genética
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Actinobacillus pleuropneumoniae/genética
Animais
Sequência de Bases
Resistência ao Cloranfenicol/genética
Dados de Sequência Molecular
Pasteurellaceae/isolamento & purificação
Análise de Sequência de DNA
Suínos
Tianfenicol/análogos & derivados
Tianfenicol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150608
[St] Status:MEDLINE


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[PMID]:26039334
[Au] Autor:Sun H; Chen H; Zang X; Hou P; Zhou B; Liu Y; Wu F; Cao X; Zhang X
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, China. sunhengyi1987@163.com.
[Ti] Título:Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum.
[So] Source:Molecules;20(6):10110-21, 2015 Jun 01.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene. First, the enhanced green fluorescent protein gene (egfp) and chloramphenicol resistance gene (Cmr), along with the two loxP loci, were integrated into the genome of A. limacinum OUC88 using 18S rDNA sequences as the homologous recombination sites. Then plasmid pSH65, containing a zeocin resistance gene (Bler) was transferred into A. limacinum OUC_CG. After induction with galactose, repeated passage in culture and PCR-based assessment, the pSH65 plasmid was lost and A. limacinum OUC_EG host was shown to no longer have resistance to 100 mg chloramphenicol/L or 5 mg zeocin/L. Through southern blotting and fluorescence detection, egfp was found to be integrated into the genome of A. limacinum OUC_EG, and EGFP was successfully expressed in the cells. The successful application of the Cre/loxP system demonstrates an experimental basis for genetic modification of A. limacinum so as to obtain transformed strains with no antibiotic resistance marker genes.
[Mh] Termos MeSH primário: Genes Bacterianos
Genoma
Integrases/genética
Recombinação Genética
Estramenópilas/genética
Transformação Genética
[Mh] Termos MeSH secundário: Bleomicina/farmacologia
Resistência ao Cloranfenicol/genética
Galactose/farmacologia
Deleção de Genes
Expressão Gênica
Engenharia Genética
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Organismos Geneticamente Modificados
Plasmídeos/química
Plasmídeos/metabolismo
RNA Ribossômico 18S/genética
RNA Ribossômico 18S/metabolismo
Estramenópilas/efeitos dos fármacos
Estramenópilas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 18S); 0 (enhanced green fluorescent protein); 11056-06-7 (Bleomycin); 147336-22-9 (Green Fluorescent Proteins); 181494-14-4 (Zeocin); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150604
[Lr] Data última revisão:
150604
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150604
[St] Status:MEDLINE
[do] DOI:10.3390/molecules200610110


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[PMID]:25552415
[Au] Autor:Jeong DE; Park SH; Pan JG; Kim EJ; Choi SK
[Ad] Endereço:Super-Bacteria Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
[Ti] Título:Genome engineering using a synthetic gene circuit in Bacillus subtilis.
[So] Source:Nucleic Acids Res;43(6):e42, 2015 Mar 31.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Genome engineering without leaving foreign DNA behind requires an efficient counter-selectable marker system. Here, we developed a genome engineering method in Bacillus subtilis using a synthetic gene circuit as a counter-selectable marker system. The system contained two repressible promoters (B. subtilis xylA (Pxyl) and spac (Pspac)) and two repressor genes (lacI and xylR). Pxyl-lacI was integrated into the B. subtilis genome with a target gene containing a desired mutation. The xylR and Pspac-chloramphenicol resistant genes (cat) were located on a helper plasmid. In the presence of xylose, repression of XylR by xylose induced LacI expression, the LacIs repressed the Pspac promoter and the cells become chloramphenicol sensitive. Thus, to survive in the presence of chloramphenicol, the cell must delete Pxyl-lacI by recombination between the wild-type and mutated target genes. The recombination leads to mutation of the target gene. The remaining helper plasmid was removed easily under the chloramphenicol absent condition. In this study, we showed base insertion, deletion and point mutation of the B. subtilis genome without leaving any foreign DNA behind. Additionally, we successfully deleted a 2-kb gene (amyE) and a 38-kb operon (ppsABCDE). This method will be useful to construct designer Bacillus strains for various industrial applications.
[Mh] Termos MeSH primário: Bacillus subtilis/genética
Redes Reguladoras de Genes
Genes Sintéticos
Engenharia Genética/métodos
[Mh] Termos MeSH secundário: Bacillus subtilis/efeitos dos fármacos
Sequência de Bases
Resistência ao Cloranfenicol/genética
DNA Bacteriano/genética
Marcadores Genéticos
Genoma Bacteriano
Dados de Sequência Molecular
Mutagênese
Óperon
Plasmídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Genetic Markers)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:151028
[Lr] Data última revisão:
151028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150102
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gku1380


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[PMID]:25085518
[Au] Autor:Casanovas-Massana A; Sala-Comorera L; Blanch AR
[Ad] Endereço:Department of Microbiology, University of Barcelona, Avinguda Diagonal, 643, 08028 Barcelona, Catalonia, Spain.
[Ti] Título:Quantification of tetracycline and chloramphenicol resistance in digestive tracts of bulls and piglets fed with Toyocerin®, a feed additive containing Bacillus toyonensis spores.
[So] Source:Vet Microbiol;173(1-2):59-65, 2014 Sep 17.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The complete genome sequencing of Bacillus toyonensis, the active ingredient of the feed additive Toyocerin(®), has revealed the presence of tetM and cat genes, a tetracycline and a chloramphenicol resistance gene, respectively. The aim of this study was to determine whether the use of Toyocerin(®) (viable spores of B. toyonensis) as a probiotic in feedstuff increased the abundance of tetracycline and chloramphenicol resistant bacteria in the intestinal tracts of piglets and Holstein bulls. To this end, qPCRs were designed to quantify the abundances of tetM and cat genes and B. toyonensis in the intestinal content of animals treated and non-treated with Toyocerin(®). Additionally, the culturable bacterial populations resistant to tetracycline or chloramphenicol were enumerated by plate counting. No statistical significances were detected between the concentrations of tetracycline or chloramphenicol resistant bacterial populations in treated and non-treated animals. The concentrations of tetM and cat in most of the treated animals were similar to those of B. toyonensis. Furthermore, tetM and cat genes were also detected in some non-treated animals, although in low concentrations. These results suggest that tetM and cat genes are already circulating among the commensal microbiota regardless of the use of Toyocerin(®). The use of Toyocerin(®) as a supplement in feedstuff does not increase the abundances of tetracycline and chloramphenicol resistant bacteria in the intestinal tracts of piglets and Holstein bulls beyond the contribution directly associated to the introduction of B. toyonensis spores through diet.
[Mh] Termos MeSH primário: Ração Animal/microbiologia
Bacillus/fisiologia
Farmacorresistência Bacteriana/genética
Trato Gastrointestinal/microbiologia
Genes Bacterianos
Probióticos/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Carga Bacteriana
Bovinos
Cloranfenicol/farmacologia
Resistência ao Cloranfenicol/genética
Masculino
Esporos Bacterianos/fisiologia
Suínos
Tetraciclina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 66974FR9Q1 (Chloramphenicol); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140826
[Lr] Data última revisão:
140826
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140803
[St] Status:MEDLINE



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