Base de dados : MEDLINE
Pesquisa : G06.099.225.937 [Categoria DeCS]
Referências encontradas : 1933 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 194 ir para página                         

  1 / 1933 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28942279
[Au] Autor:Wen X; Wang Y; Zou Y; Ma B; Wu Y
[Ad] Endereço:College of Animal Science, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
[Ti] Título:No evidential correlation between veterinary antibiotic degradation ability and resistance genes in microorganisms during the biodegradation of doxycycline.
[So] Source:Ecotoxicol Environ Saf;147:759-766, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Biodegradation of antibiotic residues in the environment by microorganisms may lead to the generation of antibiotic resistance genes (ARGs), which are of great concern to human health. The aim of this study was to determine whether there is a relationship between the ability to degrade antibiotic doxycycline (DOX) and the development of resistance genes in microorganisms. We isolated and identified ten bacterial strains from a vegetable field that had received long-term manure application as fertilizer and were capable of surviving in a series of DOX concentrations (25, 50, 80, and 100mg/L). Our results showed no evidential correlation between DOX degradation ability and the development of resistance genes among the isolated microorganisms that had high DOX degradation capability (P > 0.05). This was based on the fact that Escherichia sp. and Candida sp. were the most efficient bacterial strains to degrade DOX (92.52% and 91.63%, respectively), but their tetracycline resistance genes showed a relatively low risk of antibiotic resistance in a 7-day experiment. Moreover, the tetM of the ribosomal protection protein genes carried by these two preponderant bacteria was five-fold higher than that carried by other isolates (P < 0.05). Pearson correlations between the C /C of DOX and tet resistance genes of three isolates, except for Escherichia sp. and Candida sp., showed remarkable negative correlations (P < 0.05), mainly because tetG markedly increased during the DOX degradation process. Our results concluded that the biodegradation of antibiotic residues may not necessarily lead to the development of ARGs in the environment. In addition, the two bacteria that we isolated, namely, Escherichia sp. and Candida sp., are potential candidates for the engineering of environmentally friendly bacteria.
[Mh] Termos MeSH primário: Doxiciclina/toxicidade
Resistência Microbiana a Medicamentos/efeitos dos fármacos
Microbiologia do Solo/normas
Poluentes do Solo/toxicidade
Drogas Veterinárias/toxicidade
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Candida/efeitos dos fármacos
Candida/genética
China
Relação Dose-Resposta a Droga
Resistência Microbiana a Medicamentos/genética
Escherichia/efeitos dos fármacos
Escherichia/genética
Fertilizantes
Genes Bacterianos
Esterco/microbiologia
Resistência a Tetraciclina/efeitos dos fármacos
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Manure); 0 (Soil Pollutants); 0 (Veterinary Drugs); N12000U13O (Doxycycline)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


  2 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29395080
[Au] Autor:Gopalan A; Bhagavat R; Chandra N; Subbarao SH; Raja A; Bethunaickan R
[Ad] Endereço:Department of Immunology, National Institute for Research in Tuberculosis, Chennai, India.
[Ti] Título:Biophysical and biochemical characterization of Rv3405c, a tetracycline repressor protein from Mycobacterium tuberculosis.
[So] Source:Biochem Biophys Res Commun;496(3):799-805, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterium tuberculosis, the causative agent of tuberculosis disease, is one among the deadliest pathogens in the world. Due to long treatment regimen, HIV co-infection, persistence of bacilli in latent form and development of XDR and TDR strains of Mtb, tuberculosis has posed serious concerns for managing the disease, and calls for discovery of new drugs and drug targets. Using a computational pipeline involving analysis of the structural models of the Mtb proteome and an analysis of the ATPome, followed by a series of filters to identify druggable proteins, solubility and length of the protein, several candidate proteins were shortlisted. From this, Rv3405c, a tetR family of DNA binding protein involved in antibiotic resistance, was identified as one of the good drug targets. Rv3405c binds to the upstream non-coding region of Rv3406 and causes repression of Rv3406 activity there by affecting the downstream processes involved in antibiotic resistance was further characterized. The Rv3405c gene was cloned; the gene product was over-expressed in E. coli and purified by Ni NTA chromatography. DNA binding studies by EMSA showed that the recombinant Rv3405c protein binds to the DNA sequence corresponding to the promoter region of Rv3406 and upon addition of tetracycline, the DNA binding activity was lost. ß-galactosidase reporter assay in E. coli using both wild type and a DNA binding defective mutant protein indeed proved that Rv3405c acts as a repressor.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Proteínas de Ligação a DNA/química
Proteínas de Ligação a DNA/metabolismo
Mycobacterium tuberculosis/metabolismo
Tetraciclina/química
Tetraciclina/metabolismo
[Mh] Termos MeSH secundário: Antituberculosos/química
Sítios de Ligação
Avaliação Pré-Clínica de Medicamentos
Resistência Microbiana a Medicamentos/fisiologia
Ligação Proteica
Proteínas Repressoras
Resistência a Tetraciclina/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Bacterial Proteins); 0 (DNA-Binding Proteins); 0 (Repressor Proteins); 0 (Rv3405c protein, Mycobacterium tuberculosis); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


  3 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28698674
[Au] Autor:Li L; Kromann S; Olsen JE; Svenningsen SW; Olsen RH
[Ad] Endereço:College of Light Industry and Food Sciences, South China University of Technology, Frederiksberg C, Denmark.
[Ti] Título:Insight into synergetic mechanisms of tetracycline and the selective serotonin reuptake inhibitor, sertraline, in a tetracycline-resistant strain of Escherichia coli.
[So] Source:J Antibiot (Tokyo);70(9):944-953, 2017 Aug.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Sertraline, an antidepressive drug, has been reported to inhibit general bacterial efflux pumps. In the present study, we report for the first time a synergistic effect of sertraline and tetracycline in a TetA-encoded tetracycline-resistant strain of Escherichia coli. Synergy between sertraline and tetracycline in an E. coli strain with TetA-mediated tetracycline resistance (E. coli APEC_O2) was assessed by the MIC and checkerboard assays. The global transcriptome of E. coli APEC_O2 exposed to ½ MIC concentrations of sertraline and/or tetracycline was analyzed to elucidate the interaction mechanism between sertraline and tetracycline. The fractional inhibitory concentration index for tetracycline and sertraline in E. coli APEC_O2 was 0.5. In addition, in the presence of ½ MIC of sertraline, the sensitivity of E. coli APEC_O2 to tetracycline could be restored according to clinical standards (from 64 to 4 mg l ). RNA data suggest changes in respiration that is likely to decrease intracellular pH and thereby the proton-motive force, which provides the energy for the tetracycline efflux pump. Furthermore, sertraline and tetracycline may induce a change from oxidation to fermentation in the E.coli, which further decreases pH, resulting in cell death. This study shows that sertraline interacts with tetracycline in a synergistic and AcrAB-TolC pump-independent manner. The combinational treatment was further shown to induce many changes in the global transcriptome, including altered tetA and tetR expression. The results indicate that sertraline may be used as a helper compound with the aim to reverse tetracycline resistance encoded by tetA.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antidepressivos/farmacologia
Escherichia coli/efeitos dos fármacos
Inibidores da Captação de Serotonina/farmacologia
Sertralina/farmacologia
Resistência a Tetraciclina
Tetraciclina/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Antidepressivos/química
Biologia Computacional
Sinergismo Farmacológico
Transporte de Elétrons/efeitos dos fármacos
Complexo de Proteínas da Cadeia de Transporte de Elétrons/genética
Complexo de Proteínas da Cadeia de Transporte de Elétrons/metabolismo
Escherichia coli/crescimento & desenvolvimento
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Fermentação/efeitos dos fármacos
Perfilação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Concentração de Íons de Hidrogênio
Concentração Inibidora 50
Líquido Intracelular/efeitos dos fármacos
Líquido Intracelular/metabolismo
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Oxirredução
Força Próton-Motriz/efeitos dos fármacos
Inibidores da Captação de Serotonina/agonistas
Sertralina/agonistas
Tetraciclina/agonistas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antidepressive Agents); 0 (Electron Transport Chain Complex Proteins); 0 (Escherichia coli Proteins); 0 (Serotonin Uptake Inhibitors); F8VB5M810T (Tetracycline); QUC7NX6WMB (Sertraline)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.78


  4 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28549325
[Au] Autor:Guo MT; Zhang GS
[Ad] Endereço:State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China. Electronic address: guomeiting@tongji.edu.cn.
[Ti] Título:Graphene oxide in the water environment could affect tetracycline-antibiotic resistance.
[So] Source:Chemosphere;183:197-203, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In recent years, the influence of new materials like nanoparticles in the water environment on biological substances has been widely studied. Antibiotic resistance genes (ARGs) represent a new type of pollutant in the environment. Graphene oxide (GO), as a nano material, because of its unique structure, may have an impact on antibiotic resistance bacteria (ARB) and ARGs; however the research in this area is rarely reported. Therefore, this study mainly investigated the effects of GO on bacterial antibiotic resistance. The results showed that GO had a limited effect on ARB inactivation. A high concentration of GO (>10 mg/L) can damage resistant plasmids to reduce bacterial resistance to antibiotics, but low concentrations of GO (<1 mg/L) led to almost no damage to the plasmid. However, all tested concentrations of GO promoted the conjugative transfer from 1to over 3 folds, with low concentrations and high concentration (1-10 and 100 mg/L) of GO samples the least promoted. The overall effect of GO on antibiotic resistance needs further investigation.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Genes Bacterianos/efeitos dos fármacos
Grafite/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Farmacorresistência Bacteriana/genética
Escherichia coli/genética
Grafite/química
Viabilidade Microbiana/genética
Óxidos/química
Óxidos/farmacologia
Plasmídeos
Tetraciclina/farmacologia
Resistência a Tetraciclina/efeitos dos fármacos
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Oxides); 7782-42-5 (Graphite); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE


  5 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28535485
[Au] Autor:Yeom JR; Yoon SU; Kim CG
[Ad] Endereço:Department of Environment Engineering, INHA University, Incheon, South Korea.
[Ti] Título:Quantification of residual antibiotics in cow manure being spread over agricultural land and assessment of their behavioral effects on antibiotic resistant bacteria.
[So] Source:Chemosphere;182:771-780, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antibiotic resistant bacteria (ARB) in livestock manure used as fertilizer and spread over agriculture land, may pose a threat to the health of humans. Considering this, the concentrations of tetracycline (TC), oxytetracycline (OTC), and sulfathiazole (STZ) in the surface soil were quantified using LC-MS. These antibiotics have been used in livestock and are found in fertilizer produced from livestock excretions. Species of ABR were identified using 16S rDNA. Soil samples were collected at depths of 0, 7, and 15 cm from farmland in Incheon (South Korea). In the surface soil, three compounds were detected: TC (17.74 µg/kg), OTC (0.78 µg/kg), and STZ (0.23 µg/kg). However, except for STZ, antibiotics were not detected in the deeper samples. Overall, TC can form a chelated complex with cations, which consequently enhances its adsorption to the organic matter and metals in soil. This property can significantly reduce the mobility of TC (to lower than that of STZ). The result of 16S rDNA gene analysis indicated that Pseudomonas spp., Arthrobacter spp., and Rhodococcus spp. showed persistent resistance to the three antibiotics tested. DNA quantification results revealed strong resistance of Pseudomonas spp. to STZ, whereas Arthrobacter spp. and Rhodococcus spp. had resistance to TC and OTC. Antibiotics biodegradation suggested ability of ARB to grow in soil samples in presence of residual antibiotics during 13 days incubation. The concentrations of STZ, TC, and OTC reduced as much as 23.53, 35.60 and 66.88%, respectively.
[Mh] Termos MeSH primário: Antibacterianos/química
Bactérias/efeitos dos fármacos
Bovinos
Resíduos de Drogas/química
Esterco/análise
Microbiologia do Solo
[Mh] Termos MeSH secundário: Agricultura
Animais
Farmacorresistência Bacteriana
Feminino
República da Coreia
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE


  6 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28481346
[Au] Autor:Park J; Gasparrini AJ; Reck MR; Symister CT; Elliott JL; Vogel JP; Wencewicz TA; Dantas G; Tolia NH
[Ad] Endereço:Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
[Ti] Título:Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes.
[So] Source:Nat Chem Biol;13(7):730-736, 2017 Jul.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although tetracyclines are an important class of antibiotics for use in agriculture and the clinic, their efficacy is threatened by increasing resistance. Resistance to tetracyclines can occur through efflux, ribosomal protection, or enzymatic inactivation. Surprisingly, tetracycline enzymatic inactivation has remained largely unexplored, despite providing the distinct advantage of antibiotic clearance. The tetracycline destructases are a recently discovered family of tetracycline-inactivating flavoenzymes from pathogens and soil metagenomes that have a high potential for broad dissemination. Here, we show that tetracycline destructases accommodate tetracycline-class antibiotics in diverse and novel orientations for catalysis, and antibiotic binding drives unprecedented structural dynamics facilitating tetracycline inactivation. We identify a key inhibitor binding mode that locks the flavin adenine dinucleotide cofactor in an inactive state, functionally rescuing tetracycline activity. Our results reveal the potential of a new tetracycline and tetracycline destructase inhibitor combination therapy strategy to overcome resistance by enzymatic inactivation and restore the use of an important class of antibiotics.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Inibidores Enzimáticos/farmacologia
Legionella longbeachae/efeitos dos fármacos
Legionella longbeachae/enzimologia
Resistência a Tetraciclina/efeitos dos fármacos
Tetraciclina/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/química
Flavina-Adenina Dinucleotídeo/metabolismo
Legionella longbeachae/metabolismo
Modelos Moleculares
Conformação Molecular
Relação Estrutura-Atividade
Tetraciclina/química
Tetraciclina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enzyme Inhibitors); 146-14-5 (Flavin-Adenine Dinucleotide); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2376


  7 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28338777
[Au] Autor:Seth-Smith HM; Wanninger S; Bachmann N; Marti H; Qi W; Donati M; di Francesco A; Polkinghorne A; Borel N
[Ad] Endereço:Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Switzerland.
[Ti] Título:The Chlamydia suis Genome Exhibits High Levels of Diversity, Plasticity, and Mobile Antibiotic Resistance: Comparative Genomics of a Recent Livestock Cohort Shows Influence of Treatment Regimes.
[So] Source:Genome Biol Evol;9(3):750-760, 2017 Mar 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chlamydia suis is an endemic pig pathogen, belonging to a fascinating genus of obligate intracellular pathogens. Of particular interest, this is the only chlamydial species to have naturally acquired genes encoding for tetracycline resistance. To date, the distribution and mobility of the Tet-island are not well understood. Our study focused on whole genome sequencing of 29 C. suis isolates from a recent porcine cohort within Switzerland, combined with data from USA tetracycline-resistant isolates. Our findings show that the genome of C. suis is very plastic, with unprecedented diversity, highly affected by recombination and plasmid exchange. A large diversity of isolates circulates within Europe, even within individual Swiss farms, suggesting that C. suis originated around Europe. New World isolates have more restricted diversity and appear to derive from European isolates, indicating that historical strain transfers to the United States have occurred. The architecture of the Tet-island is variable, but the tetA(C) gene is always intact, and recombination has been a major factor in its transmission within C. suis. Selective pressure from tetracycline use within pigs leads to a higher number of Tet-island carrying isolates, which appear to be lost in the absence of such pressure, whereas the loss or gain of the Tet-island from individual strains is not observed. The Tet-island appears to be a recent import into the genome of C. suis, with a possible American origin.
[Mh] Termos MeSH primário: Infecções por Chlamydia/genética
Chlamydia/genética
Genômica
Resistência a Tetraciclina/genética
[Mh] Termos MeSH secundário: Animais
Chlamydia/patogenicidade
Infecções por Chlamydia/microbiologia
Infecções por Chlamydia/veterinária
Gado/genética
Gado/microbiologia
Testes de Sensibilidade Microbiana
Plasmídeos/genética
Suínos/genética
Suínos/microbiologia
Tetraciclina/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx043


  8 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28314124
[Au] Autor:Yang Y; Liu W; Xu C; Wei B; Wang J
[Ad] Endereço:Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
[Ti] Título:Antibiotic resistance genes in lakes from middle and lower reaches of the Yangtze River, China: Effect of land use and sediment characteristics.
[So] Source:Chemosphere;178:19-25, 2017 Jul.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Freshwater lakes provided an ideal media for the accumulation and propagation of antibiotic resistance genes (ARGs), because they were susceptible to anthropogenic impacts. Land reclamation and urbanization exerted severe anthropogenic impacts on lakes from middle and lower reaches of the Yangtze River, China over the past decades. In this study, 15 lakes in the region were selected to understand the level and variability of ARGs. Proportion of different land use types was applied to display the land reclamation and urbanization around each lake. For sulfonamide resistance (sul) genes, sul1 had the highest relative abundance in sediments, with maximum 2.11 × 10 copies/16SrRNA copy in Gehu Lake. For tetracycline resistance (tet) genes, tetG had the highest average value of relative abundance (4.74 × 10 copies/16SrRNA copy), followed by tetB, tetA, tetQ and tetM. Class I integron (intI1) played an important role in acquisition and dissemination of sul1 and tetG. Sediment characteristics (moisture, density, total nitrogen, total carbon, ammonium, and nitrate) were found to have no significant effect on ARG distribution. Taihu Lake and Yangcheng Lake which exhibited high sul and tet genes had the high proportion of built-up land use.
[Mh] Termos MeSH primário: Resistência Microbiana a Medicamentos/genética
Monitoramento Ambiental/métodos
Sedimentos Geológicos/química
Lagos/microbiologia
Rios/microbiologia
Urbanização
[Mh] Termos MeSH secundário: China
Genes Bacterianos/genética
Integrons
Sulfonamidas
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfonamides)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170524
[Lr] Data última revisão:
170524
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE


  9 / 1933 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28290267
[Au] Autor:Martini CL; Lange CC; Brito MA; Ribeiro JB; Mendonça LC; Vaz EK
[Ad] Endereço:University of Santa Catarina State, Center of Agroveterinary Sciences,Lages, Santa Catarina,Brazil.
[Ti] Título:Characterisation of penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine milk samples in Minas Gerais, Brazil.
[So] Source:J Dairy Res;84(2):202-205, 2017 May.
[Is] ISSN:1469-7629
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This Regional Research Communication describes the characterisation of ampicillin, penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine subclinical mastitis in Minas Gerais State, Brazil. Ninety S. aureus isolates from bovine mastitis exhibiting phenotypic resistance to ampicillin, penicillin and/or tetracycline were selected for this study. The minimum inhibitory concentration (MIC) of each antibiotic was determined using the E-Test® and the production of beta-lactamase was determined by cefinase disks. The resistance genes blaZ, tet(K), tet(L), tet(M), and tet(O) were investigated by PCR in all of the isolates. The MIC results classified 77, 83 and 71% of the isolates as resistant to ampicillin, penicillin and tetracycline, respectively. The MIC50 and MIC90 were, respectively, 1 and 2 µg/ml for ampicillin, 0·5 and 1 µg/ml for penicillin and 32 and 64 µg/ml for tetracycline. Eighty-six per cent of beta-lactamase producing isolates were detected. Of the 90 isolates investigated, 97% amplified blaZ, 84% amplified tet(K), 9% amplified tet(L), 2% amplified tet(M) and 1% amplified tet(O). Seventy-nine isolates (88%) showed blaZ together with at least one tet gene. S. aureus isolates showed high MIC50 and MIC90 values for the three antimicrobials. The blaZ and tet(K) genes were widespread in the herds studied, and most of the isolates harboured blaZ and tet(K) concomitantly.
[Mh] Termos MeSH primário: Mastite Bovina/microbiologia
Leite/microbiologia
Resistência às Penicilinas
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/isolamento & purificação
Resistência a Tetraciclina
[Mh] Termos MeSH secundário: Ampicilina/administração & dosagem
Animais
Brasil
Bovinos
Feminino
Testes de Sensibilidade Microbiana
Resistência às Penicilinas/genética
Penicilinas/administração & dosagem
Reação em Cadeia da Polimerase/veterinária
Staphylococcus aureus/genética
Tetraciclina/administração & dosagem
Resistência a Tetraciclina/genética
beta-Lactamases/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Penicillins); 7C782967RD (Ampicillin); EC 3.5.2.6 (beta-Lactamases); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1017/S0022029917000061


  10 / 1933 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28285864
[Au] Autor:Ishag HZ; Xiong Q; Liu M; Feng Z; Shao G
[Ad] Endereço:Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing 210014, China; College of Veterinary Sc
[Ti] Título:E. coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis.
[So] Source:J Microbiol Methods;136:49-56, 2017 May.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mycoplasma hyorhinis is an opportunistic pathogen of pigs. Recently, it has been shown to transform cell cultures, increasing the attention of the researchers. Studies on the pathogenesis require specific genetic tool that is not yet available for the pathogen. To address this limitation, we constructed two suicide plasmids pGEMT-tetM/LR and pGEMT-recA-tetM/LR having a tetracycline resistance marker flanked by two hemolysin gene arms. The latter plasmid encodes an E. coli recA, a gene involved in DNA recombination, repair and maintenance of DNA. Using inactivation of the hemolysin gene, which results in a detectable and measurable phenotype, we found that each plasmid can disrupt the hemolysin gene of M. hyorhinis through a double cross-over homologous recombination. However, inclusion of the E. coli recA gene in the construct resulted in 9-fold increase in the frequency of hemolysin gene mutants among the screened tetracycline resistance colonies. The resultant hemolysin mutant strain lacks the ability to lyse mouse bed blood cells (RBC) when tested in vitro (p<0.001). The host-plasmid system described in this study, has applications for the genetic manipulation of this pathogen and potentially other mycoplasmas.
[Mh] Termos MeSH primário: Escherichia coli/genética
Marcação de Genes/métodos
Recombinação Homóloga
Mycoplasma hyorhinis/genética
Recombinases Rec A/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
DNA Bacteriano
Genes Bacterianos
Vetores Genéticos
Proteínas Hemolisinas/genética
Camundongos
Mutação
Mycoplasma/genética
Infecções por Mycoplasma/diagnóstico
Mycoplasma hyorhinis/patogenicidade
Fenótipo
Plasmídeos
Regiões Promotoras Genéticas
Proteínas Recombinantes/genética
Recombinação Genética
Reparo de DNA por Recombinação
Suínos
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Hemolysin Proteins); 0 (Recombinant Proteins); EC 2.7.7.- (Rec A Recombinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE



página 1 de 194 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde